\n<\/td>\n | lane 14: mm ruler<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n lane 15: uni ruler<\/p><\/blockquote>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_6345.jpg,",33.8610309,-118.2547536,04/30/2025," dragonfly was collected at California Academy of Mathematics and Science. Weather between 55-65 Fahrenheit.<\/p>\n",Arthropoda,DNeasy (Qiagen),Abdomen,MiniOne,,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_5667.jpg,"," lane 2: mm ruler<\/p>\n lane 3: uni. ruler<\/p>\n lane 5: long leg spider<\/p>\n lane 6: roly poly<\/p>\n lane 7: dragon fly<\/p>\n lane 8: black aphids<\/p>\n lane 9: (+) arthro control<\/p>\n lane 10: (-) arthro control<\/p>\n lane 11: (+) DNA control<\/p>\n lane 12: water<\/p>\n lane 14: mm ruler<\/p>\n lane 15: uni. ruler<\/p>\n",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/DSC10228-1-scaled.jpg,",43.4871498859,-111.9872730833,04/07/2025," Sample code: LW16<\/p>\n",Adalia frigida,Edwards Buffer,Whole arthropod,Standard electrophoresis system,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1st_gel.jpg,","",Yes,TGAGCAGGAATAGTAGGAACTTCTCTAAGAATTATTATTCGATTAGAATTAGGTACAACTAACAGACTAATTGGAAATGACCAAATTTATAATGTTATTGTAACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCCATTATAATTGGAGGTTTTGGAAACTGACTAGTACCTTTAATAATTGGAGCGCCTGATATGGCATTCCCACGTCTTAATAATATAAGATTTTGATTATTACCTCCAGCTTTAACCCTTTTAATTTCTAGAAGAGTAATTGAAATAGGAGCAGGTACAGGATGAACAGTATATCCACCTCTTTCTTCAAATATAGCACATAATGGACCTTCTGTAGATTTAGTAATTTTTAGATTACACTTAGCTGGAATTTCATCAATTTTAGGAGCTGTAAATTTTATTTCTACTATTATAAATATACGACCTAATGGGATAAATCTAGATAAAACACCTTTATTTGTTTGATCAGTTTTAATTACAGCTATTCTACTACTTTTATCATTACCAGTTCTTGCAGGAGCAATTACTATATTATTAACAGATCGTAATATTAATACATCATTTTTTGACCCTACTGGAGGAGGGGATCCAGTTTTATACCAACACTTATTTTGATTTTT,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/DSC10201-scaled.jpg,",43.5541523,-111.8804601,01/24/2025," caught deceased<\/p>\n sample code: LW5<\/p>\n",,Edwards Buffer,Whole arthropod,Standard electrophoresis system,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1_29_25_Gel_Image_Cropped.jpg,","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/LW2_2-1-scaled.jpg,",43.48715667629995,-111.98726192224464,01/20/2025," caught deceased<\/p>\n Sample code: LW2<\/p>\n",Pollenia pediculata,Edwards Buffer,Whole arthropod,Standard electrophoresis system,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1_22_25_Gel_Image_Cropped-1.jpg,","",Yes,TTTCGGAGCNTGATCAGGAATAGTAGGTACCTCTTTAAGAATATTAGTTCGAGCAGAATTAGGACATCCTGGAGCATTAATCGGTGATGATCAAATTTATAATGTAATTGTAACAGCCCATGCCTTTGTTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGACTTGTTCCTTTAATATTAGGAGCACCTGATATAGCATTCCCTCGAATGAATAATATAAGTTTTTGACTCTTACCTCCTGCACTTACATTATTATTGGTGAGCAGTATAGTGGAAAACGGAGCTGGGACAGGATGAACTGTTTACCCACCTCTATCTTCTAATATTGCTCATGGAGGGGCTTCTGTTGATTTAGCTATTTTTTCTCTTCATTTAGCAGGAATTTCTTCAATTTTAGGAGCAGTTAATTTTATTACAACTGTAATTAATATACGATCTACAGGTATTACATTTGACCGAATACCTTTATTTGTTTGATCTGTAGTAATTACAGCATTATTACTTCTTTTATCCTTACCTGTATTAGCAGGAGCTATCACTATATTATTAACAGATCGAAATTTAAATACTTCCTTTTTTGATCCTGCAGGAGGAGGAGACCCAATTTTATATCAACATTTATTTTGATTTTT,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/LW1_2-1-scaled.jpg,",43.4871685845,-111.9872938453,," caught deceased.<\/p>\n",Armadillidium vulgare,Edwards Buffer,Whole arthropod,Standard electrophoresis system,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1_22_25_Gel_Image_Cropped.jpg,","",Yes,TTGGGGTTTGAGCAGGGGCTGTTGGGACTGCCCTTAGAATAATTATTCGCACTGAATTAGGACAGCCTGGAAGTTTAATTGGAGATGATCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATACCTATTATAATTGGTGGATTCGGTAACTGGTTAATTCCATTAATACTAGGAGCCCCAGATATAGCTTTTCCACGGATAAATAATATAAGATTTTGACTTTTACCCCCTTCTTTAACTTTATTACTTAGAAGGGGATTAGTTGAAAGAGGAGTAGGAACAGGGTGGACAGTATATCCTCCGCTGGCGTCGAGAATTGCTCACAGAGGTGCATCTGTAGATTTAGGTATTTTTTCTCTCCACCTTGCTGGAGCTTCTTCTATTTTAGGGGCTGTAAATTTTATTACTACTATAATTAATATACGAGCAGCTGGAATCAGAATAGACCGTGTTCCTTTATTTGTTTGATCAGTAATAGTAACGGCTGTGCTTTTGCTTTTATCATTACCTGTACTAGCAGGAGCTATTACTATATTATTAACTGATCGAAATTTAAATACCTCTTTTTTTGACCCTAGCGGAGGTGGGGATCCTATCCTTTATCAACATTTATTCTGATTTTT,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/8B746587-4CB3-4B71-BBE6-736D5CF38CB3.jpg,",33.8611082147,-118.2549120610,05/09/2025," Bug was found on the leaves of a bush.<\/p>\n",Murgantia histrionica,DNeasy (Qiagen),Body without legs,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-28-9.21.14-AM.png,"," Gel electrophoresis was run successfully and bands of harlequin bug DNA were present.<\/p>\n",No,NNNNNNNNNNNNNNTGNNNNNNNANANNNGGTATAGTTGGTACTTCTTTAAGAATCCTAATTCGCCTAGAACTAGGAACA ACTAATAGATTAATTGGAAACGACCAAATTTATAATGTAATTGTTACTGCTCATGCATTTATTATAATTTTTTTCATAGT AATACCTATTATAATTGGAGGATTTGGAAATTGACTAGTACCATTAATAATTGGCGCTCCAGATATAGCTTTCCCACGTT TAAATAATATAAGATTTTGACTTTTGCCTCCCGCATTAACATTATTAATAATAAGAATAATTGTAGAAATAGGGGCAGGC ACAGGTTGAACAGTCTACCCTCCACTATCTTCAAATTTGGCACATAATGGACCCTCTGTTGATTTAGTAATTTTTAGTTT ACATTTAGCTGGAATTTCGTCTATTTTAGGGGCTGTTAATTTTATTTCAACTATTATAAATATACGCCCTTCGGGAATAA GATTAGATAAAACTCCCTTATTTGTTTGATCAGTTATGATCACAGCTATTTTATTACTTTTATCTTTACCTGTATTAGCA GGAGCCATCACTATACTTTTAACAGATCGAAATATCAATACTTCATTTTTTGACCCTACGGGAGGGGGAGACCCTATTCT TTACCAACATTTATTCTGATTTTTTGGTCACCCTTGAAANTNNN,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/schoolbug.png,",33.9547370000,-118.2120161000,05/01/2025," At home, found in the backyard under mulch.<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen) blood and tissue kit.,Abdomen,MiniPCR,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/COlGel.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/wolbachia.png,","",No,NNNNNNNNNTTTGGGGTTTGAGCAGGGGCTGTTGGAACTGCCCTTAGAATAATTATTCGCACTGAATTAGGACNACCTGG AAGTTTAAATGGAGATGACCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATAC CTATTATAATTGGTGGATTTGGTAATTGGTTAATTCCATTAATACTAGGAGCCCCAGATATGGCTTTTCCACGAATAAAT AATATAAGATTTTGACTTTTACCCCCTTCTTTAACTTTATTGCTTAGAAGTGGGTTGGTTGAAAGAGGAGTAGGAACAGG ATGGACAGTATATCCTCCGCTGGCGTCTAGAATTGCTCATAGAGGAGCATCTGTAGATTTAGGTATTTTTTCTCTCCACC TTGCTGGAGCTTCTTCTATTTTAGGGGCTGTGAATTTTATTACTACTATAATTAATATACGAGCAGCTGGAATTAGAATA GACCGTGTTCCTTTATTTGTTTGATCAGTATTAGTAACGGCTGTACTTTTACTTTTATCCCTACCTGTATTAGCAGGAGC TATTACTATATTATTAACTGATCGAAATTTAAATACCTCTTTTTTTGACCCTAGGGGAGGAGGAGACCCTATCCTTTATC AACACTTATTTTGATTTTTTGGTCACCCTGAAGTTNAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/35_Pillbug-CO1F.fa,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/D138CD77-4743-4E0F-81B4-556E6F42D48A.jpg,",33.8611823162,-118.2547760815,05/09/2025," Insect was found under a discarded piece of wood.<\/p>\n",Neuroptera,DNeasy (Qiagen),Larva,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-28-9.21.14-AM-1.png,"," While a gel was run with Plodia Interpunctella samples\/DNA gel electrophoresis was not run properly\/ samples were not prepared correctly so no bands appeared on the gel.<\/p>\n",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/2E6305BC-43F2-40E0-88E8-A02D6D15728E.jpg,",33.8611925135,-118.2547222531,05/09/2025," Insect was found in leaf litter that came from a nearby tree.<\/p>\n",Forficula auricularia,DNeasy (Qiagen),Rear half,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-28-9.21.14-AM-1.png,"," While a gel was run with Forficula auricularia samples\/DNA gel electrophoresis was not run properly\/ samples were not prepared correctly so no bands appeared on the gel.<\/p>\n",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/D-nemesis-hen.png,",33.8612330463,-118.2547387536,05/09/2025," Insect was found crawling in grass when collected.<\/p>\n",Harmonia axyridis,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-28-9.21.14-AM.png,"," While a gel was run with Harmonia Axyridis samples\/DNA gel electrophoresis was not run properly\/ samples were not prepared correctly so no bands appeared on the gel.<\/p>\n",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_3006-2.jpeg,",118.2551400000,33.8608000000,05/12/2025," \n- The arthropod was caught in a garden.<\/li>\n
- It was green and had long legs.<\/li>\n<\/ul>\n",Condylostylus,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_0664-scaled.jpeg,","
The arthropods were caught and then ground to proceed to the DNA extraction and purification process. Once our experimental and control samples were prepped, we moved on to the PCR protocol. Samples and our PCR cocktail were added to each of our PCR tubes, which were then put into our MiniOne PCR machine. Finally, loading dye was added to the separated portions of our PCR samples and was loaded into our gel. Our final gel was stained with 100X Fast Blast and put onto a light box for imaging.<\/span><\/p>\n",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screen-Shot-2025-05-19-at-9.42.26-AM.png,",33.8607775366,-118.2551858141,05/12/2025,"The pill bug was found in the soil of our school’s garden. It was also attempting to dig into the soil. It had a hard exoskeleton, was about the size of a grain of rice, and was dark gray.<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screen-Shot-2025-05-19-at-9.38.42-AM.png,"," The arthropods were caught and then ground to proceed to the DNA extraction and purification process. We did come across an issue with our second arthropod sample containing the pill bug, as the exoskeleton was not removed to lessen the risk of being unable to extract sufficient DNA. This led to our spin column being clogged for a significant amount of time, which we fixed by moving the sample around with a pipette tip. However, this might have decreased the efficiency of our DNA sample. Once our experimental and control samples were prepped, we moved on to the PCR protocol. Samples and our PCR cocktail were added to each of our PCR tubes, which were then put into our MiniOne PCR machine. Finally, loading dye was added to the separated portions of our PCR samples and was loaded into our gel. Our final gel was stained with 100X Fast Blast and put onto a light box for imaging.<\/p>\n",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/spider.png,",33.8612294210,-118.2553503156,04/28/2025," We found this grass spider in the grass near bushes. It was collected in the late morning, between 50-60 degrees F.<\/p>\n",Agelenidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/wolbachia-gel-edited.png,"," DNA Extraction:\u00a0<\/strong>The arthropod was crushed thoroughly to ensure proper extraction of DNA.<\/p>\nGel Electrophoresis Lanes for Arthropod Gel:<\/strong><\/p>\n1 – PCR molecular ruler (ladder)<\/p>\n 2 – Black-Bodies Fruit Fly<\/p>\n 3 – Roly Poly<\/p>\n 4 – Grass Spider<\/p>\n 5 – Long-Legged Fly<\/p>\n 6 – (+) Arthropod Control<\/p>\n 7 – (-) Arthropod Control<\/p>\n 8 – (+) DNA Control<\/p>\n Analysis<\/strong>:<\/strong> Our controls worked confirming our trial worked correctly and we had bands confirming that our insect samples are arthropods.<\/p>\nGel Electrophoresis Lanes for Wolbachia Gel:<\/strong><\/p>\n1 – PCR molecular ruler (ladder)<\/p>\n 2 – Black-Bodies Fruit Fly<\/p>\n 3 – Roly Poly<\/p>\n 4 – Grass Spider<\/p>\n 5 – Long-Legged Fly<\/p>\n 6 – (+) Arthropod Control<\/p>\n 7 – (-) Arthropod Control<\/p>\n 8 – (+) DNA Control<\/p>\n Analysis<\/strong>:<\/strong> Our did not work meaning our trial was not successful and there were no bands on our gel except for our ladder meaning that our run is inconclusive most likely due to Wolbachia primers not working correctly.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/roly-poly.png,",33.8619529226,-118.2547688797,04/28/2025,"We found this roly poly under some bark and leaves. It was collected in the late morning, between 50-60 degrees F. Many different insects and more roly polies were present but we only collected one.<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen) blood and tissue kit,Rear half,Standard electrophoresis system Bio-Rad,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/wolbachia-gel-edited.png,"," DNA Extraction:\u00a0<\/strong>The arthropod was crushed thoroughly to ensure proper extraction of DNA.<\/p>\nGel Electrophoresis Lanes for Arthropod Gel:<\/strong><\/p>\n1 – PCR molecular ruler (ladder)<\/p>\n 2 – Black-Bodies Fruit Fly<\/p>\n 3 – Roly Poly<\/p>\n 4 – Grass Spider<\/p>\n 5 – Long-Legged Fly<\/p>\n 6 – (+) Arthropod Control<\/p>\n 7 – (-) Arthropod Control<\/p>\n 8 – (+) DNA Control<\/p>\n Analysis<\/strong>:<\/strong> Our controls worked confirming our trial worked correctly and we had bands confirming that our insect samples are arthropods.<\/p>\nGel Electrophoresis Lanes for Wolbachia Gel:<\/strong><\/p>\n1 – PCR molecular ruler (ladder)<\/p>\n 2 – Black-Bodies Fruit Fly<\/p>\n 3 – Roly Poly<\/p>\n 4 – Grass Spider<\/p>\n 5 – Long-Legged Fly<\/p>\n 6 – (+) Arthropod Control<\/p>\n 7 – (-) Arthropod Control<\/p>\n 8 – (+) DNA Control<\/p>\n Analysis<\/strong>:<\/strong> Our did not work meaning our trial was not successful and there were no bands on our gel except for our ladder meaning that our run is inconclusive most likely due to Wolbachia primers not working correctly.<\/p>\n <\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/fly.png,",33.8610928319,-118.2553225552,04/28/2025," We found this black-bodied fruit fly by sweeping some bushes with a net. It was collected in the late morning, between 50-60 degrees F.<\/p>\n",Drosophila melanogaster,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/arthropod-gel-edited.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/wolbachia-gel-edited.png,"," DNA Extraction:\u00a0<\/strong>The arthropod was crushed thoroughly to ensure proper extraction of DNA.<\/p>\nGel Electrophoresis Lanes for Arthropod Gel:<\/strong><\/p>\n1 – PCR molecular ruler (ladder)<\/p>\n 2 – Black-Bodies Fruit Fly<\/p>\n 3 – Roly Poly<\/p>\n 4 – Grass Spider<\/p>\n 5 – Long-Legged Fly<\/p>\n 6 – (+) Arthropod Control<\/p>\n 7 – (-) Arthropod Control<\/p>\n 8 – (+) DNA Control<\/p>\n Analysis<\/strong>:<\/strong> Our controls worked confirming our trial worked correctly and we had bands confirming that our insect samples are arthropods.<\/p>\nGel Electrophoresis Lanes for Wolbachia Gel:<\/strong><\/p>\n1 – PCR molecular ruler (ladder)<\/p>\n 2 – Black-Bodies Fruit Fly<\/p>\n 3 – Roly Poly<\/p>\n 4 – Grass Spider<\/p>\n 5 – Long-Legged Fly<\/p>\n 6 – (+) Arthropod Control<\/p>\n 7 – (-) Arthropod Control<\/p>\n 8 – (+) DNA Control<\/p>\n Analysis<\/strong>:<\/strong> Our did not work meaning our trial was not successful and there were no bands on our gel except for our ladder meaning that our run is inconclusive most likely due to Wolbachia primers not working correctly.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/long-legged-fly.png,",33.8612311299,-118.2553338442,04/28/2025,"This long-legged fly was found after sweeping some bushes with a net. It was collected in the late morning in 50-60 degree F weather.<\/p>\n",Condylostylus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system Bio-Rad,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/arthropod-gel-edited.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/wolbachia-gel-edited.png,"," DNA Extraction:\u00a0<\/strong>The arthropod was crushed thoroughly to ensure proper extraction of DNA.<\/p>\nGel Electrophoresis Lanes for Arthropod Gel:<\/strong><\/p>\n1 – PCR molecular ruler (ladder)<\/p>\n 2 – Black-Bodies Fruit Fly<\/p>\n 3 – Roly Poly<\/p>\n 4 – Grass Spider<\/p>\n 5 – Long-Legged Fly<\/p>\n 6 – (+) Arthropod Control<\/p>\n 7 – (-) Arthropod Control<\/p>\n 8 – (+) DNA Control<\/p>\n Analysis<\/strong>:<\/strong> Our controls worked confirming our trial worked correctly and we had bands confirming that our insect samples are arthropods.<\/p>\nGel Electrophoresis Lanes for Wolbachia Gel:<\/strong><\/p>\n1 – PCR molecular ruler (ladder)<\/p>\n 2 – Black-Bodies Fruit Fly<\/p>\n 3 – Roly Poly<\/p>\n 4 – Grass Spider<\/p>\n 5 – Long-Legged Fly<\/p>\n 6 – (+) Arthropod Control<\/p>\n 7 – (-) Arthropod Control<\/p>\n 8 – (+) DNA Control<\/p>\n Analysis<\/strong>:<\/strong> Our did not work meaning our trial was not successful and there were no bands on our gel except for our ladder meaning that our run is inconclusive most likely due to Wolbachia primers not working correctly.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_5664.jpg,",33.8611020614,-118.2547534232,04/30/2025,"Spider was collected at California Academy of Mathematics and Science. Weather between 55-65 Fahrenheit.<\/p>\n",Arachnida,DNeasy (Qiagen),Abdomen,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_5667.jpg,"," lane 2: mm ruler<\/p>\n lane 3: uni. ruler<\/p>\n lane 5: long leg spider<\/p>\n lane 6: roly poly<\/p>\n lane 7: dragon fly<\/p>\n lane 8: black aphids<\/p>\n lane 9: (+) arthro control<\/p>\n lane 10: (-) arthro control<\/p>\n lane 11: (+) DNA control<\/p>\n lane 12: water<\/p>\n lane 14: mm ruler<\/p>\n lane 15: uni. ruler<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_9378-scaled.jpg,",33.8536990380,-118.3995014165,05/04/2025," Sand crab was collected along the Redondo Beach shoreline inside the sand when waves came up (early afternoon). Weather was overcast and around 65 degrees F. No environmental image was taken.<\/p>\n",Decapoda,DNeasy (Qiagen),Abdomen,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_0173-scaled.jpg,"," DNA Ladder in well 1 \nEmerita analoga sample 1 (A) in well 3, sample 2 (B) in well 4, and sample 3 (D) in well 6<\/p>\n Controls run from wells 10-13 (Arthropod +, Arthropod -, DNA +, and DNA – respectively)<\/p>\n 6X UView stain used for all samples, controls, and ladder. 1X TAE Buffer used in the gel casting and electrophoresis chamber.<\/p>\n",No,NNNNNNNNNNNNNTNNNANNNNNNNCTGGGATAGTGGGCACTTCCTTAAGACTTATTATTCGAGCAGAGTTAGGCCAACC AGGAAGACTAATTGGGGACGACCAGATTTATAATGTCATTGTCACTGCCCACGCATTTGTTATAATTTTCTTTATAGTTA TACCGATCATAATCGGGGGATTTGGTAATTGGCTAGTACCTCTTATATTAGGTGCCCCAGACATAGCTTTTCCGCGTATA AACAATATAAGATTCTGACTTTTGCCCCCTTCCCTAACACTTCTTCTCATGAGTGGAATAGTGGAAAGAGGAGTTGGTAC GGGATGGACAGTTTATCCTCCCCTATCAGCAGCAATCGCCCACGCGGGGGCTTCAGTAGACCTTGGTATTTTCAGACTTC ACCTGGCCGGGGTCTCATCCATTCTAGGAGCAGTGAATTTTATAACTACAGTTATTAATATACGACCCAATGGGATAACA ATGGACCGTATACCTCTATTTGTGTGATCAGTGTTCATTACAGCAATTTTACTTTTACTATCGCTACCAGTTTTAGCGGG AGCTATTACCATGCTTTTGACCGACCGTAANTTAAACACCTCTTTTTTTTGATCCGGCCGGAGGGGGAGACCCAGTTCTA TATCAACACCNGTTCNGATTTTTTGGTCACCCNTGNAAGTNTNNANNGTCNNN,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/31_PacificSandCrab-CO1F.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_9379-scaled.jpg,",33.8549196854,-118.3998232816,05/04/2025," Sand crab was collected along the Redondo Beach shoreline inside the sand when waves came up (early afternoon). Weather was overcast and around 65 degrees F. No environmental image was taken.<\/p>\n",Decapoda,DNeasy (Qiagen),Abdomen,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_0173-scaled.jpg,"," DNA Ladder in well 1 \nEmerita analoga sample 1 (A) in well 3, sample 2 (B) in well 4, and sample 3 (D) in well 6<\/p>\n Controls run from wells 10-13 (Arthropod +, Arthropod -, DNA +, and DNA – respectively)<\/p>\n 6X UView stain used for all samples, controls, and ladder. 1X TAE Buffer used in the gel casting and electrophoresis chamber.<\/p>\n",No,NNNNNNNNNNNNNNNCNNAGNNNNNNCTGGGATAGTGGGCACTTCCTTAAGACTTATTATTCGAGCAGAGTTAGGCAAAC CAGGAAGACTAATTGGGGACGACAAGATTTATAATGTCATTGTCACTGCCCACGCGTTTGTTATAATTTTCTTTATAGTT ATACCGATCATAATCGGGGGATTTGGTAATTGGCTAGTACATCTTATATTAGGTGCCCCAGACATAGCTTTTCCACGTAT AAACAATATAAGATTCTGACTTTTGCCCCCTTCCCTAACACTTCTTCTCATGAGTGGAATANTGGAAAGAGGAGTTGGTA CGGGATGGACAGTTTATCCTCCCANATCATCANCTCTCGCCTATTGGANGGCTTGCCNAGCCCTTGGTATTTATTGATTT CNNTCCCTAANNNNAGCATCATTATNGGANNNGGGANTTTTATTTNACTNACATTTTTATTTATTANATGTATTNCC,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/32_PacificSandCrab-CO1F.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_9380-scaled.jpg,",33.8537435875,-118.3997374509,05/04/2025," Sand crab was collected along the Redondo Beach shoreline inside the sand when waves came up (early afternoon). Weather was overcast and around 65 degrees F. No environmental image was taken.<\/p>\n",Decapoda,DNeasy (Qiagen),Abdomen,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_0173-scaled.jpg,"," DNA Ladder in well 1 \nEmerita analoga sample 1 (A) in well 3, sample 2 (B) in well 4, and sample 3 (D) in well 6<\/p>\n Controls run from wells 10-13 (Arthropod +, Arthropod -, DNA +, and DNA – respectively)<\/p>\n 6X UView stain used for all samples, controls, and ladder. 1X TAE Buffer used in the gel casting and electrophoresis chamber.<\/p>\n",No,NNNNNNNNNCNCNNNNNNNNNNNNCTGGGATAGTGGGCACTTCNNNNAGACTTATTATTCGAGCAGAGTTAGGCCAACCA GGAAGACTAATTGGGGACGACCAGATTTATAATGTCATTGTCACTGCCCACGCATTTGTTATAATTTTCTTTATAGTTAT ACCGATCATAATCGGGGGATTTGGTAATTGGCTAGTACCTCTTATATTAGGTGCCCCAGACATAGCTTTTCCACGTATAA ACAATATAAGATTCTGACTTTTGCCCCCTTCCCTAACACTTCTTCTCATGAGTGGAATAGTGGAAAGAGGAGTTGGTACG GGATGGACAGTTTATCCTCCCCTATCAGCAGCAATCGCCCACGCGGGGGCTTCAGTAGACCTTGGTATTTTCAGACTTCA CNNGGCCGGGGTCTCATCCATTCTAGGAGCAGTGAATTTTATAACTACAGTTATTAATATACGACCCAATGGGATAACAA TGGACCGTATACCTCTATTTGTGTGATCAGTGTTTCATTACAGCAATTTTACTTTTACTATCGCTACCANTTTTAGCGGG AGCTATTACCATGCNTTTNGACCGATCGTAATTTAAACACCTCTTTTTTTTGATCCGGCCNGGAGGGGGGAGACCCCAGT TCNANANCAACACCTGTTTCTGANTTTTTTGGGTCACCNNGAANNTTAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/33_PacificSandCrab-CO1F.fasta,,
"",39.8827229986,-75.7195956164,05/09/2025,"",Attulus fasciger,tweezers,Whole arthropod,MiniOne,TBE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-05-at-12.31.41 PM-3.png,","",Yes,,,,
"",39.8467767000,-75.7116032000,05/09/2025,"",Coccinellidae,tweezers,Abdomen,MiniOne,TBE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-05-at-12.31.41 PM-2.png,","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/homebug.jpg,",33.8316745000,-118.2816930000,05/01/2025," On the surface of school garden, found underneath leaves.<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen) blood and tissue kit.,Abdomen,MiniPCR,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/COlGel.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/wolbachia.png,"," N\/A<\/p>\n",No,NNNNNNNNGNNTNNGNGGTTTGAGCAGGGGCTGTTGGAACTGCCCTTAGAATAATTATTCGCACTGAATTAGGACAACCT GGAAGTTTAATTGGAGATGACCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTAAT ACCTATTATAATTGGTGGATTTGGTAATTGGTTAATTCCATTAATACTAGGAGCCCCAGATATGGCTTTTCCACGAATAA ATAATATAAGATTTTGACTTTTACCCCCTTCTTTAACTTTATTGCTTAGAAGTGGGTTGGTTGAAAGAGGAGTAGGAACA GGATGGACAGTATATCCTCCGCTGGCGTCTAGAATTGCTCATAGAGGAGCATCTGTAGATTTAGGTATTTTTTCTCTCCA CCTTGCTGGAGCTTCTTCTATTTTAGGGGCTGTGAATTTTATTACTACTATAATTAATATACGAGCAGCTGGAATTAGAA TAGACCGTGTTCCTTTATTTGTTTGATCAGTATTAGTAACGGCTGTACTTTTACTTTTATCCCTACCTGTATTAGCAGGA GCTATTACTATATTATTAACTGATCGAAATTTAAATACCTCTTTTTTTGACCCTAGGGGAGGAGGAGACCCTATCCTTTA TCAACACTTATTTTGATTTTTTGGTCACCNNNNNNNTTNA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/34_Pillbug-CO1F.fa,,
"",39.8467767,-75.7116032,,"",Drosophila melanogaster,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniOne,TBE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-21-2.21.30-PM.png,"," Our controls and DNA ladder passed through the gel correctly and had the expected results.\u00a0 We have also spent many months perfecting our technique and perfecting and mistakes.\u00a0 This fruit fly was a cultivated control that was infected with Wolbachia, and tested positively for Wolbachia.<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Armadillidium-vulgare-1.png,",33.8607762370,-118.2552319000,05/02/2025,"",Armadillidium vulgare,DNeasy (Qiagen),Whole arthropod,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-19-175851.png,"," 5. PCR molecular weight ruler (1000, 700, 500, 200, 100 bp)<\/p>\n 6. Water<\/p>\n 7. <\/em>Positive DNA Control<\/p>\n8. DNA sample of Armadillidium vulgare<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/fruitfly-1.jpg,",39.8467767,-75.7116032,05/01/2025,"",Drosophila melanogaster,tweezers,Whole arthropod,MiniOne,TBE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-05-at-12.31.41 PM-1.png,","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_6780-scaled.jpeg,",33.8609101000,-118.2553106000,05/05/2025," Black, has many legs, looks like pillbug<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_1245-scaled.jpg,"," Armadillidium vulgare of interest is in the fourth lane from the left. PCR with Wolbachia 16S primers was performed, so the band indicates that the organism was positive for Wolbachia.<\/span><\/p>\n",Yes,NNNNNNNNNNNNNNNNNNNNNNNGCAGGGGCTGTTGGAACTGCCCTTAGA ATAATTATTCGCACTGAATTAGGACAACCTGGAAGTTTAATTGGAGATGA CCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTT TTATAGTAATACCTATTATAATTGGTGGATTTGGTAATTGGTTAATTCCA TTAATACTAGGAGCCCCAGATATGGCTTTTCCACGAATAAATAATATAAG ATTTTGACTTTTACCCCCTTCTTTAACTTTATTGCTTAGAAGTGGGTTGG TTGAAAGAGGAGTAGGAACAGGATGGACAGTATATCCTCCGCTGGCGTCT AGAATTGCTCATAGAGGAGCATCTGTAGATTTAGGTATTTTTTCTCTCCA CCTTGCTGGAGCTTCTTCTATTTTAGGGGCTGTGAATTTTATTACTACTA TAATTAATATACGAGCAGCTGGAATTAGAATAGACCGTGTTCCTTTATTT GTTTGATCAGTATTAGTAACGGCTGTACTTTTACTTTTATCCCTACCTGT ATTAGCAGGAGCTATTACTATATTATTAACTGATCGAAATTTAAATACCT CTTTTTTTGACCCTAGGGGAGGAGGAGACCCTATCCTTTATCAACACTTA TTTTGATTTTTTGGTCNNNTNGGAAGTTNNA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Armadillidium-vulgare-1.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_1185-scaled.jpeg,",33.860908047834975,-118.25527120617723,05/05/2025,"Appears to be a pillbug<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_1245-scaled.jpg,"," Armadillidium vulgare of interest is in the fifth lane from the left. PCR with Wolbachia 16S primers was performed, so the band indicates that the organism was positive for Wolbachia.<\/p>\n",Yes,NNNTNNNNNNNNNNNNNNNNNNNANAGCAGGGGCTGTTGGAACTGCCCTT AGAATAATTATTCGCACTGAATTATGGACAACCTGGAAGTTTAATTGGAG ATGACCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATT TTTTTTATAGTAATACCTATTATAATTGGTGGATTTGGTAATTGGTTAAT TCCATTAATACTAGGAGCCCCAGATATGGCTTTTCCACGAATAAATAATA TAAGATTTTGACTTTTACCCCCTTCTTTAACTTTATTGCTTAGAAGTGGG TTGGTTGAAAGAGGAGTAGGAACAGGATGGACAGTATATCCTCCGCTGGC GTCTAGAATTGCTCATAGAGGAGCATCTGTAGATTTAGGTATTTTTTCTC TCCACCTTGCTGGAGCTTCTTCTATTTTAGGGGCTGTGAATTTTATTACT ACTATAATTAATATACGAGCAGCTGGAATTAGAATAGACCGTGTTCCTTT ATTTGTTTGATCAGTATTAGTAACGGCTGTACTTTTACTTTTATCCCTAC CTGTATTAGCAGGAGCTATTACTATATTATTAACTGATCGAAATTTAAAT ACCTCTTTTTTTGACCCTAGGGGAGGAGGAGACCCTATCCTTTATCAACA CTTATTTTGATTTTTTGGTCACCCNTGAAGTTNA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Armadillidium-vulgare-2.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Armadillidium-vulgare-2.png,",33.8607762370,-118.2552319000,05/02/2025,"",Armadillidium vulgare,DNeasy (Qiagen),Whole arthropod,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-19-175943-1.png,"," 1. PCR molecular weight ruler (1000, 700, 500, 200, 100 bp)<\/p>\n 2. DNA sample of Pholcus phalangioides<\/p>\n 3. DNA sample of Pholcus phalangioides<\/em><\/p>\n4. DNA sample of Armadillidium vulgare<\/p>\n 5. Duplex positive control<\/p>\n 6. Duplex negative control<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_2277-scaled.jpeg,",33.8614214000,-118.2544495000,04/30/2025,"",Isopoda,DNeasy (Qiagen),Abdomen,Standard electrophoresis system Bio-Rad,1X TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screen-Shot-2025-05-19-at-1.17.56-PM.png,","",No,NNNNNNNNNNNNNNNNGGTANGANNNNNNGTNNNTGAGGTGCTTGCNCANT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/42_SpotlessLadybugPillBug-CO1F.fa,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-14-at-11.38.31 AM.png,",33.8610309,-118.2547536,04/30/2025," Location: CAMS bushes<\/p>\n Season: Spring<\/p>\n Temperature: 62\u00b0F<\/p>\n Habitat: Terrestrial<\/p>\n \n- pointy tail<\/li>\n
- attatched on leaf<\/li>\n
- very still<\/li>\n<\/ul>\n",Coleoptera,tweezers,Abdomen,Standard electrophoresis system Bio-Rad,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-19-at-1.05.03 PM.png,","",No,NNNNNNNNNNGNNTTTGGGGTTTGAGCAGGGGCTGTTGGAACTGCCCTTAGAATAATTATTCGCACTGATTTTTGGGTCN CCCTGAATTTNANTGGAGATGACCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTA ATACCTATTATAATTGGTGGATTTGGTAATTGGTTAATTCCATTAATACTAGGAGCCCCAGATATGGCTTTTCCACGAAT AAATAATATAAGATTTTGACTTTTACCCCCTTCTTTAACTTTATTGCTTAGAAGTGGGTTGGTTGAAAGAGGAGTAGGAA CAGGATGGACAGTATATCCTCCGCTGGCGTCTAGAATTGCTCATAGAGGAGCATCTGTAGATTTAGGTATTTTTTCTCTC CACCTTGCTGGAGCTTCTTCTATTTTAGGGGCTGTGAATTTTATTACTACTATAATTAATATACGAGCAGCTGGAATTAG AATAGACCGTGTTCCTTTATTTGTTTGATCAGTATTAGTAACGGCTGTACTTTNACTTTTATCCCTACCTGTATTANCAG GAGCTATTACTATATTATTAACTGATCGAAATTTAAATACCTCTTTTTTTGACCCTAGGGGAGGAGGAGACCCTATCCTT TATCAACACTTATTTTGATTTTTTGGTCACCCTTGANNTTTAAA,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-21-at-10.58.01 AM.png,",33.8614214,-118.2544495,04/30/2025,"
\n- found on bush<\/li>\n
- located in leaf at the top of the bush<\/li>\n<\/ul>\n",Diptera,DNeasy (Qiagen),Abdomen,Standard electrophoresis system Bio-Rad,1X TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screen-Shot-2025-05-19-at-1.17.56-PM.png,","",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_1184-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_1183-scaled.jpeg,",33.8609007857,-118.2552553841,05/05/2025,"
Striped abdomen, red eyes, fly<\/p>\n",Minettia flaveola,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_1245-scaled.jpg,"," Minettia flaveola of interest is in the third lane from the left. PCR with Wolbachia 16S primers was performed, so the lack of band indicates that the organism was negative for Wolbachia.<\/p>\n",No,NNNNNNNNNNNNNNNNGNNNNNNNNNNCCGGATAGTAGGAACATCTTTAA GAATTTTAATTCGTGCTGAATTAGGACACCCAGGTGCCTTAATTGGAGAC GACCAAATTTATAATGTTATTGTTACAGCCCATGCATTTGTTATAATTTT TTTTATAGTTATACCAATTATAATTGGAGGATTTGGAAATTGACTAGTCC CTTTAATATTAGGAGCTCCTGATATGGCATTTCCACGAATAAATAATATA AGATTTTGATTATTACCTCCTTCCCTTACATTACTTTTAGTAAGAAGTAT AGTTGAAAACGGAGCTGGAACAGGATGAACTGTATACCCTCCGTTATCAT CAGGAATTGCTCACGGAGGAGCATCTGTTGATTTAGCTATTTTTAGTCTT CATTTAGCTGGGGCTTCATCAATTATAGGAGCAGTAAATTTTATTACTAC AGTCATTAACATACGATCAACTGGAATCACTTTTGATCGAATACCTTTAT TTGTATGATCTGTTGTAATTACTGCTATTCTTTTACTTCTTTCTCTACCT GTTTTAGCAGGAGCAATCACTATGTTATTAACAGATCGAAATTTAAATAC TTCATTTTTTGACCCAGCGGGTGGAGGAGATCCAATTTTATACCAACATT TATTTTGATTTTTTGGTCACCNNNGNANTTNNN,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Minettia-flaveola.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_6783-scaled.jpeg,",33.860910387859064,-118.25530367879078,05/05/2025," shiny green fly<\/p>\n",Condylostylus,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_1245-scaled.jpg,"," Condylostylus of interest is in the second lane from the left. PCR with Wolbachia 16S primers was performed, so the band indicates that the organism was positive for Wolbachia.<\/span><\/p>\n",Yes,NNNNNNNNNNNNCNNNNNNNNNNNNNNNNGGATAATTGGTACATCCCTTA GAATTATTGTACGAGCTGAATTAGGACATCCAGGAGCTCTAATTGGAGAT GACCAAATTTATAATGTTATTGTAACAGCTCACGCATTTATCATAATTTT CTTTATAGTAATACCAATCATAATTGGAGGGTTTGGAAATTGATTAGTTC CCCTTATATTAGGAGCTCCTGATATAGCATTCCCACGAATAAATAATATA AGATTCTGAATACTCCCCCCATCATTAACCCTACTACTAGCTAGTAGTAT AGTAGAAAATGGAGCAGGAACAGGATGAACAGTATATCCCCCTTTATCAG CAGGAATTGCTCACGGAGGTGCATCTGTTGACTTAGCAATTTTTTCTCTT CATTTAGCTGGTATTTCATCTATTTTAGGAGCAGTAAATTTTATTACAAC AGTAATTAATATACGATCAACAGGAATCACTCTAGATCGAATACCATTAT TTGTATGATCAGTAGTAATTACAGCTGTTCTTCTTCTCCTTTCATTACCA GTTCTAGCCGGAGCTATTACCATATTACTAACTGACCGAAATTTAAATAC TTCATTTTTTGACCCAGCAGGAGGAGGTGACCCAATTCTATATCAACATT TATTTTGATTTTTTGGTCACNCNNNANNNTNNN,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Condylostylus.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Pholcus-phalangioides-2.png,",33.8607762370,-118.2552319000,05/02/2025,"1. PCR molecular weight ruler (1000, 700, 500, 200, 100 bp)<\/p>\n 2. DNA sample of pholcus phalangioides<\/p>\n 3. DNA sample of pholcus phalangioides<\/em><\/p>\n4. DNA sample of armadillidium vulgare<\/p>\n 5. Duplex positive control<\/p>\n 6. Duplex negative control<\/p>\n",Pholcus phalangioides,DNeasy (Qiagen),Body without legs,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-19-175943.png,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/cricket-pic.png,",33.9127807000,-118.3520389000,05/01/2025," Found in a group under a basketball hoop in a backyard at night. It was roughly 65 degrees Fahrenheit.<\/p>\n",Gryllus,DNeasy (Qiagen),Partial abdomen,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/wolbachia-gel-pic-1.png,"," Lane 1: Long Beach cricket 1, Lane 3: Long beach cricket 2, Lane 5: Hawthorne cricket 1, Lane 7: Hawthorne cricket 2<\/p>\n",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_0438.jpeg,",33.8863314000,-118.2935891000,05/14/2025," I found this cellar spider below a mirror on the wall in a household bathroom. I collected it at about 8 am.<\/p>\n <\/p>\n
<\/p>\n",Pholcus phalangioides,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-20-9.34.48-AM-1.png,"," Gel electrophoresis lanes<\/strong> \n1. PCR molecular weight ruler (1000, 700, 500, 200, 100 bp) \n2. DNA sample of pholcus phalangioides \n3. DNA sample of sphaerophoria scripta<\/em> \n4. Wolbachia-positive (16S) arthropod control \n5. Wolbachia-negative (16S) arthropod control \n7. Arthropod-positive (CO1) DNA control \n8. Arthropod-negative (CO1) DNA control<\/p>\nAnalysis<\/strong><\/p>\nThe pholcus phalangioides sample had a band matching the Wolbachia-positive control’s band (16S), but not a band matching the arthropod-positive control’s upper band (CO1; like how the arthropod-negative control had no bands), so pholcus phalangioides seems to be a non-arthropod and Wolbachia-positive.<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_0440.jpeg,",33.8863314000,-118.2935891000,05/14/2025," I found this long hoverfly in an open compost bag with fruit and vegetable scraps buried in soil. I collected it at about 5 pm when it was about 68 degrees Fahrenheit.<\/p>\n",Syrphidae,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-20-9.34.48-AM-1.png,"," Gel electrophoresis lanes<\/strong> \n1. PCR molecular weight ruler (1000, 700, 500, 200, 100 bp) \n2. DNA sample of pholcus phalangioides \n3. DNA sample of sphaerophoria scripta<\/em> \n4. Wolbachia-positive (16S) arthropod control \n5. Wolbachia-negative (16S) arthropod control \n7. Arthropod-positive (CO1) DNA control \n8. Arthropod-negative (CO1) DNA control<\/p>\nAnalysis<\/strong><\/p>\nThe sphaerophoria scripta sample had a band matching the arthropod-positive control’s upper band (CO1), but not a band matching the Wolbachia-positive control’s band (16S; like how the Wolbachia-negative control had no bands), so sphaerophoria scripta seems to be an arthropod and Wolbachia-negative.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/download.jpg,",39.8467767000,-75.7116032000,,"",Chironomus tentans,tweezers,Whole arthropod,MiniOne,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-05-at-12.31.28 PM.png,","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_3707.png,",33.8612302770,-118.2553553772,04/30/2025,"",Coleoptera,DNeasy (Qiagen),Abdomen,MiniOne,1X TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screen-Shot-2025-05-19-at-1.17.56-PM.png,","",Yes,NNNNNNNNNNNNNNNNNNNNTNNNNNNNNNGNNTGNCGNNTCNCNNNGAANNNGNNNNNNNNNGGNNANNNNGNNNCCNN NNNNNNTTNNNNNCATCCTNNTCTTACCNAATTGCTCACGGNGGNAGAATGNCCCCGTCACTGCCCCTGTAAGAGTGTNG GNGGNNGGNGNTCAAATATNATCGGGNGTTGGGGGTGGGTGATNGACNNCATNCATTNGAGGCTATNANATANTCTTTTN NTTNACTAACTAAAGTAANAANNTATCTTANTACCCCNNCTTTATTTTTTATTGNTTACNANNAANTTGGTGTGAANNNN ATTAATANCNAGANGNNTNNTTNTTTTTTTTGCTGGTNTTTTNTTCNTTTTTTTTTTTTTTANCNNNNNNCNNNNGNNNN NNNNNTNNNNNTTANNNTANNNATNNANNNTTTNNTNNNN,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/28_RolyPolySpotlessLadybug-CO1F-1.fa,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Pholcus-phalangioides-1-1.png,",33.8607762375,-118.2552319790,05/02/2025,"",Pholcus phalangioides,DNeasy (Qiagen),Body without legs,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/59C5A188-23D1-4AAF-A63E-A88CB7E79450.jpg,"," 1. PCR molecular weight ruler (1000, 700, 500, 200, 100 bp)<\/p>\n 2. DNA sample of Pholcus phalangioides<\/p>\n 3. DNA sample of Pholcus phalangioides<\/em><\/p>\n4. DNA sample of Armadillidium vulgare<\/p>\n 5. Duplex positive control<\/p>\n 6. Duplex negative control<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/B8_tanytarsus_1-scaled.jpg,",46.5481898880,9.9003661667,10/02/2024,"",Tanytarsus pallidicornis,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Gel_B8_tanytarsus_bearbeitet-scaled.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/B8_Co1-F.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/B4_camponotus.jpg,",46.5487359087,9.9006665742,10/02/2024,"",Camponotus herculeanus,Instagene Matrix,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Gel_B4_camponotus_bearbeitet-scaled.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/B4_Co1-F.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/centi2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/centi.jpg,",33.8614214000,-118.2544495000,04/30/2025," Found under shrubbery, in a damp and dark environment.<\/p>\n",Lamyctes emarginatus,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/WolbachiaGel.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Ladder-Gel.jpg,"," Gel 1 \nLane \n1 – PCR MW Ladder (failed) \n2 – centipede (lamyctes emarginatus) \n3 – pillbug (armadillidium vulgare) \n4 – woodlouse spider (dysdera crocata) \n5 – ground beetle (anisodactylus binotatus) \n6 – Arthropod Positive Control \n7 – Arthropod Negative Control \n8 – Drosophila Positive Control \n9 – Drosophila Negative Control<\/p>\n Gel 2 \nLane \n3 – PCR MW Ruler \n6 – MiniOne Ladder<\/p>\n Our first gel’s ladder did not run successfully, so we ran another gel with the same ladder and and another MiniOne ladder and compared the relative mobilities of the two gels to determine the base pairs of the first gel.<\/p>\n",No,NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNTTGNNACNNCNCNNNNNNNNNNTNNNNATCCACCNNCCCCNNNGN NGGNATTCTTNTGGGACACACGTAATTGTCTGGACACTANTCGCCCCCACNCTTTAANTANNATTTTGTNTGGATTACGT CCATCATAAATGGNGTTTGGAAGNNGGCACTTNCNTNTACATCGGTGGNGCNGACGGNNNCCCCNTCNNCCAAATAAAAA ACTAATNTATTAGTANCNCCCCCCCACTCANTN,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/21_Centipede-CO1F_D05.fa,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Bild_2025-04-30_150532360.png,",47.4596592677,9.0194997356,03/19/2025," unknown<\/p>\n",Ochlerotatus sticticus,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Image-11.jpg,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Bild_2025-04-30_150054179.png,",47.4592977760,9.0228878876,03/19/2025," unknown<\/p>\n",Myrmica rubra,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Image-10.jpg,","",Yes,AGAATAATTATTCGTKTAGAATTAGGATCTTGYAACCCATTAATTAATAATGATCAAATTTATAATTCTCTTGTAACTAGCCATGCCTTTATTATAATTTTTTTTATAGTTATACCATTTATAATTGGGGGCTTTGGTAATTTTTTAATTCCTTTAATATTAGGATCACCTGATATAGCATACCCACGAATAAATAACATAAGATTTTGATTACTTCCCCCTTCAATTATACTATTAATATTAAGAAATTTTTTAAGGAATGGTGTAGGTACAGGATGAACCATTTACCCCCCTCTAGCTTCTAATATTTTCCATAGAGGACCTTCAATTGATATATCAATCTTTTCTCTTCATATCGCTGGAATATCATCTATTTTAGGTGCTATTAATTTTATCTCAACAATTATTAATATACATCATAAATCAGTATCAATAGACAAAATCCCCTTAATAGTCTGATCAATTTTAATCACAGCTATTCTTCTCTTACTTTCCTTACCAGTCTTAGCAGGAGCAATTACTATATTATTAACAGATCGTAATTTAAATACTTCTTTTTTCGACCCATCTGGGGGGGGAGATCCAATTTTATACCAACATCTATTTTGATTTTTTGGTCACCTGAAAAGTTWAA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/DS1_CO1.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Bild-20250319-150222-1e237a68.jpeg,",47.4597798902,9.0191428750,03/19/2025," I found the Lasius niger nearby woodstacks in the forest directly by a path. The weather was sunny and it was around 15 degrees outside. The place was very dry and full of wood chips. It was in the afternoon, about 3pm. There were a lot of leaves around the place, where I found the Lasius niger. And it was autumn.<\/p>\n",Lasius niger,Instagene Matrix,Body without legs,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Biologie_Wolbachiapraktikum_Elektrophorese.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/NB2_CO1.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Bild-20250319-145528-5c952cc7.jpeg,",47.4597841871,9.0195362068,03/19/2025," I found this the Pterostichus niger under a trunk in the forest near a path. I collected it in the afternoon, about 3pm. It was a sunny day and it was about 15 degrees outside. The area around the trunk was very dirty and wet. The Pterostichus niger was hiding in the dirt 5cm underneath the ground.<\/p>\n <\/p>\n",Pterostichus niger,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Biologie_Wolbachiapraktikum_Elektrophorese.jpg,","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/NB1_CO1.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Chelidurella-acanthopygia-FS-1-1.jpg,",47.4602413801,9.0190972767,03/19/2025," Forest ground<\/p>\n",Chelidurella acanthopygia,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Gel-Image-FS-LR-1.jpg,","",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Lasius-Niger-Internet.jpg,",47.4603800401,-350.9809385283,03/19/2025," Forest ground<\/p>\n",Lasius niger,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Gel-Image-FS-LR-1.jpg,","",No,ACTAGAATTAAGTTCTTCTAATTCATTAATTAATAATGATCAAATTTATAATTCTATAGTTACAAGACATGCATTCGTTATAATTTTCTTTATAGTTATGCCTTTCATAATTGGAGGATTTGGAAATTTTCTCGTACCTTTAATATTAGGATCACCTGATATAGCATACCCCCGTATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATTTCTCTCCTTCTTTTAAGAAATTTTATTAATGACGGAGAGGAACAGGATGAACTGTTTATCCTCCATTAGCTTCTAATATTTTTCATAATGGCCCTCAGTTGATTTAACTATTTTTTCTCTTCATATTGCTGGA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/FS2_CO1.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BildFM2.1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BildFM2.2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BildFM2.3.jpg,",47.4592753789,9.0234843045,03/19/2025," unknown<\/p>\n",Anoecia fulviabdominalis,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/ElektroFM.jpeg,"," Band is not interpretable<\/p>\n",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BildFM1.1-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BildFM1.2-2.jpg,",47.4593078345,9.0229746466,03/19/2025," unknown<\/p>\n",Ochlerotatus sticticus,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/ElektroFM.jpeg,"," <\/p>\n Band is not interpretable<\/p>\n",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/AMs2025d-scaled.jpg,",41.7756400000,-88.0970000000,09/03/2024," collected by Fall 2024 students<\/p>\n",Bombus impatiens,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-07-at-11.00.39 PM.png,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/crayfish.jpg,",43.466503654165706,-112.07092282778625,04/01/2025," Subject was caught alive in a freshwater aquatic (pond) environment.<\/p>\n",Pollenia pediculata,Edwards Buffer,Partial abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/PCR2.jpeg,"," The sample gel electrophoresis tests are located in the bottom gel, rightmost column, fourth and eighth rows (WspecF and CO1, respectively) (see gel EP image). The shakiness of the bars in the Gel EP suggests imperfections in the gel structure, most likely during formation.<\/p>\n The DNA extraction was performed on the reproductive organs of the sample (female).<\/p>\n",No,CCCTGGAGCATTAATCGGTGATGATCAAATTTATAATGTAATTGTAACAGCCCATGCCTTTGTTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGACTTGGAAATTGACTTGTTCCTTTAATATTAGGAGCACCTGATATAGCATTCCCTCAAATGAATAATATAATTTTTTGACTCTTACCTCCTGCNCTNACTTTATTATTGGTGAGCAGTATAGTGGAAAACGGAGCTGGGACAGGATGAACTGTTTACCCACCTCTATCTTCTAATATTGCTCATGGAGGGGCTTCTGTTGATTTAGCTATTTTTTCTCTTCATTTAGCAAGAATNTCTTCTCTTTTAGGAGCAGTTAATTTTATTACNACTGTATTTAATATACNATCTACAGGTATTACATTTGACCGCNTACCTTTATTTGTTTTATCTGTANTAATTACNGCCTTATTAATTTTT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/ZL6_CO1.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/spider.jpg,",43.4233213000,-112.0143728000,04/01/2025," Caught alive in a suburban environment, isolated from its web.<\/p>\n",Tegenaria,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/PCR2.jpeg,"," The\u00a0Tegenaria<\/em> tests are located in the bottom gel, rightmost column, third and seventh rows (WspecF and CO1, respectively). The shakiness of the bars in the Gel EP suggests imperfections in the gel structure, most likely during formation.<\/p>\n",No,AAAGTGATGATTCTAATTGAATTGGGTCAGTTGGGGAGATTTATTGGAGATGATCATTTATATAATGTAATTGTTACGGCTCACGCTTTTGTTATGATTTTTTTTATAGTGATACCTATTATAATTGGTGGGTTTGGAAATTGATTGGTTCCTTTAATATTAAGGGCTCCGGATATGGCTTTTCCTCGAATGAATAATTTGAGATTTTGATTGTTGCCTCCTTCGTTATTTATGCTTTTTATTTCTTCTATGGTGGATATAGGGGTTGGTGCTGGTTGAACAATTTATCCTCCTTTGGCTTCTTCTATGGGACATATAGGGAGAGCTATAGATTTTGCTATTTTTTCTTTGCATTTAGCAGGGGCGTCTTCTATTATGGGAGCGGTGAATTTTATTACTACGATTTTAAATATACGTTCTGTAGGGATAAGAATAGATAGGGTTTCTTTGTTTGTTTGGTCGGTATTGGTTACCGCTATTTTATTGTTATTGTCTTTGCCTGTATTAGCGGGTGCTATTACGATATTGTTGACGGATCGAAATTTTAATACATCATTTTTTGATCCTGCAGGTGGAGGGGATCCGATTTTATTTCAACACTTGTTTTGATTTTTTGGTCACCCTGAA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/ZL5_CO1.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/whitefly_egg.jpg,",43.4233213000,-112.0143728000,04/01/2025,"Was found inhabiting a lemon tree in a residential suburban environment. Found and captured in egg stage.<\/p>\n",Trialeurodes vaporariorum,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/PCR2.jpeg,"," The\u00a0Trialeurodes vaporariorum<\/em> tests are located in the bottom gel, rightmost column, second and sixth rows (WspecF and CO1, respectively). The shakiness of the bars in the Gel EP suggests imperfections in the gel structure, most likely during formation.<\/p>\n",No,ATGTTCTTGTAACTTCCCATGCATTTATTATAATTTTTTTTATGACTATGCCTCTTGTTATTGGTGGGTTCGGGAACTGGCTGGTTCCTCTTATGGTTGGGGCTCCTGATATGGCGTTTCCTCCAATAAATAATCTTAGATTTTGGCTGTTGNTTCCTTCTTTGTTTTTTATGCTTGTTAGACTTTTGATTGATAGGGGAAGCGGCACGGGTTGAACTGTTTATCCCCCCCTATCTATAAGGCTGTCACATAGGGGGAATTCTGTCGATTTTTCTATTATNTCTTTGCACGTTGCTGGAATTTCTTCTATTTTGGGATCACTTAATTTTATCCTGACTATTGTAAACATGCAGGCGTTGGGCATAAAAATGGAGTTTTAATCTTTGTTTGTCTGATCTGTGTTTATTACTGTTTTCTTGCTTTTAATTTCTCTTCCTGTGTGGGCAGGGGCAATTACTATACTTTTACTGGATNGTAATTTTAA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/ZL4_CO1.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Ameise-1-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Ameise-2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Ameise-3.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Ameise-4.jpg,",474620.3200000000,90132.8100000000,03/05/2025,"I found it in a little forest, about 3 pm, in a field path with gravel, at a sunny spot. It was about 10-15 degrees. I didn’t find alot of them, just one. The one I collected<\/p>\n",Lasius niger,Instagene Matrix,Body without legs,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Gel-1.jpg,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Assel-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Assel-2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Assel-3.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Assel-4.jpg,",474620.3200000000,90132.8100000000,03/05/2025," I found it at a little forest. It was about 3 pm, with 10-15 degrees. Under a dry conifer in the shadow. There were alot of them there.<\/p>\n",Porcellio scaber,Instagene Matrix,Rear half,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Gel-2.jpg,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Image-13.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Image-14.jpg,",474620.3200000000,90132.8100000000,03/05/2025," I found it in a little forest. It was at about 3pm. It was under a conifer bark. It was 10 to 15 degrees. There were 4 of this bugs but I took one.<\/p>\n",Rhagium inquisitor,Instagene Matrix,Rear half,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Gel-bearbeitet.jpg,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1000005745-scaled.jpg,",43.4807784,-112.0117657,,"",Isopoda,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/isopod2-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/isopod.jpg,","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1000005741-scaled.jpg,",43.4233213000,-112.0143728000,,"",Chironomus riparius,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/harlequin.jpg,","",No,TGGGAACTTCATTAAGAATGCTTATTCGAGCAGAATTAGGACGACCCGGAACTTTCATTGGAGATGACCAAATTTATAATGTTGTAGTTACTGCACATGCTTTTATTATAATTTTTTTCATAGTTATACCAATTTTAATTGGAGGATTCGGAAACTGACTTGTCCCCCTAATACTTGGAGCACCTGACATAGCTTTTCCTCGAATAAATAATATAAGTTTCTGACTTTTACCCCCCTCTCTTACTCTTCTTCTTTCTAGTTCTTTCGTAGAAAATGGAGCAGGAACTGGGTGAACTGTTTATCCACCTCTATCATCAGCAATTGCTCATAGAGGAGCTTCTGTTGACTTAGCTATTTTTTCTTTACATTTAGCTGGAATTTCATCAATTCTTGGATCAGTAAATTTTATTACTACAGTTATTAATATGCGAGCTAATGGTATTACACTTGATCGAATACCTTTATTTGTCTGATCAGTAGTAATTACAACTGTTCTTTTACTTCTTTCTCTTCCAGTCTTAGCTGGAGCCATTACTATACTATTAACAGACCGAAATTTAAATACTTCATTTTTTGATCCTGCTGGTGGGGGTGATCCTATTTTATACCAACACTTATTTTGATT,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1000005743-scaled.jpg,",43.4233213,-112.0143728,,"",Pterostichus,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/beetle.jpg,","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1000005742-scaled.jpg,",43.4233213000,-112.0143728000,04/13/2025,"",Forficula auricularia,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Earwig.jpg,","",No,GGCTTGATCAGGGATAGTAGGGACTTCATTGAGTTTGTTGATTCGAGCAGAGTTGGGACAACCTGGAGCTTTAATTGGAGACGATCAAATTTATAATGTAATTGTTACGGCCCACGCTTTCGTAATGATTTTTTTTATGGTAATGCCTATTATGATTGGGGGTTTTGGGAATTGATTGGTACCCCTGATGCTGAGAGCCCCTGACATGGCCTTTCCTCGAATAAATAATATAAGTTTTTGGTTACTACCTCCTTCGCTTATACTTTTGCTCTCTGGGAGAATGGTAGACAGAGGGGCAGGAACGGGTTGGACGGTCTACCCTCCCCTGTCGGGGGCTATCGCTCACGCAGGGGCTTCGGTGGATTTGAGAATTTTTTCATTGCACTTGGCGGGGGTTTCATCCATTTTAGGGGCAATTAATTTTATTACAACGGTGATTAACATGCGCCCCTCAGGTCTTAAATTGGAACGAATACCATTATTTGTGTGATCTGTAGCCATTACTGCTCTTTTATTGTTACTTTCTTTACCAGTGTTGGCAGGGGCTATCACTATGCTTTTGACAGACCGGAATTTAAATACATCTTTTTTTGACCCTGCGGGAGGGGGGGACCCTATTCTCTATCAACATTTATTTTGATTTTTTG,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1000005661-scaled.jpg,",43.4233213000,-112.0143728000,,"",Chironomus riparius,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/chiron.jpg,","",No,GNATAGTGGGAACTTCATTAAGAATGCTTATTCGAGCAGAATTAGGACGACCCGGAACTTTCATTGGAGATGACCAAATTTATAATGTTGTAGTTACTGCACATGCTTTTATTATAATTTTTTTCATAGTTATACCAATTTTAATTGGAGGATTCGGAAACTGACTTGTCCCCCTAATACTTGGAGCACCTGACATAGCTTTTCCTCGAATAAATAATATAAGTTTCTGACTTTTACCCCCCTCTCTTACTCTTCTTCTTTCTAGTTCTTTCGTAGAAAATGGAGCAGGAACTGGGTGAACTGTTTATCCACCTCTATCATCAGCAATTGCTCATAGAGGAGCTTCTGTTGACTTAGCTATTTTTTCTTTACATTTAGCTGGAATTTCATCAATTCTTGGATCAGTAAATTTTATTACTACAGTTATTAATATGCGAGCTAATGGTATTACACTTGATCGAATACCTTTATTTGTCTGATCAGTAGTAATTACAACTGTTCTTTTACTTCTTTCTCTTCCAGTCTTAGCTGGAGCCATTACTATACTATTAACAGACCGAAATTTAAATACTTCATTTTTTGATCCTGCTGGTGGGGGTGATCCTATTTTATACCAACACTTATTTTGA,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1000005662-scaled.jpg,",43.4807879,-112.0117695,04/01/2025,"",Steatoda triangulosa,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/steatoda-1.jpg,","",No,ATAATTGGAACAGGTATGAGTGTTTTAATTCGGGTAGAATTAGGACAATCTGGAAGATTGTTAGGTGATGATCAATTATATAATGTAATTGTTACTGGACACGCTTTTGTGATGATTTTTTTTATAGTAATACCAGTATTAATTGGTGGATTTGGAAATTGGTTGGTTCCATTAATATTAGGGGCTCCTGATATAGCTTTTCCTCGAATAAATAATTTAAGATTTTGATTATTACCTCCTTCTTTATTATTATTATTAATTTCTTCTATAGAAGAAATAGGTGTTGGAGCTGGATGAACTATTTACCCCCCTTTATCTTCTCTGGAAGGTCATAGAGGAAGATCTGTAGATTTTGCTATTTTTTCTTTACATTTGGCTGGTGCTTCTTCCATTATAGGTGCTATTAATTTTATTTCTACTATTTTAAATATACGTTTATTTGGGATGAGAATAGAAAAAGTTAGACTTTTTGTTTGGTCAGTATTAATCACTGCAGTATTATTATTATTATCTTTACCTGTATTGGCAGGTGCCATTACTATATTATTAACAGATCGAAATTTTAATACTTCTTTTTTTGACCCGGCTGGGGGAGGTGATCCAATTTTATTTCAACATTTGTTTTGATTTTT,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Isopod-Elisabet-Cabrera-Caballero-27-Feburary-2-scaled.jpg,",38.8196500000,-77.2619567000,03/09/2025," Brown pillbug with pale yellow-beige accents caught in an urban forest during spring.<\/p>\n",Trachelipus rathkii,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Latin-III.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-06-at-8.48.41 PM.png,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/DSC10233-scaled.jpg,",43.4233213000,-112.0143728000,04/01/2025," Arthropod was collected alive in an interior suburban environment.<\/p>\n",Chrysoperla,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/RPCR1-1.jpg,","",Yes,ATTTGATCTGGATTAGTAGGTACAAGATTAAGTTTATTGATTCGAGCTGAATTAGGTCAACCAGGTTCTTTAATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTAATACCTATTGTAATTGGAGGTTTTGGTAATTGATTAGTTCCTTTAATACTAGCTGCTCCTGATATAGCTTTTCCACGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTTTATTACTTGCTTCTTCTATAGTAGAAAGAGGAGCTGGGACTGGTTGAACAGTTTACCCTCCTTTATCATCAAGAATTGCACATGCTGGAGCTTCTGTGGATTTAGCTATTTTTAGTTTACACCTTGCCGGAATCTCATCAATTTTAGGTGCAGTAAATTTCATTACAACAGTAATTAATATACGATTAAGTTATATAACATTAGACCGTATACCATTATTTGTATGATCAGTAGTAATTACAGCTTTATTATTATTACTTTCATTACCTGTATTAGCGGGTGCTATTACTATGTTATTAACTGATCGTAATTTAAATACTTCATTTTTTGATCCTGCAGGAGGAGGAGATCCAATTTTATATCAACATTTATTTTGATTTTTTGGTCACCC,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/ZL2_CO1-1.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/DSC10232-scaled.jpg,",43.4233213000,-112.0143728000,04/01/2025," Found inside of my house, recently deceased, surrounded by several others.<\/p>\n",Harmonia axyridis,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/RPCR1.jpg,","",No,TTGGATATGGGCAGGAATAGTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAGAGGAAGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTTTGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTTTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGATAAAACACCTTTATTTGTATGATCTGTTCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCATTTACTATACTATTAACTGAC,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/ZL1_CO1.fasta,,
"",38.8176997269,-77.1685620830,,"",,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Mahnoor-K_IMG_0001-scaled.jpg,",41.7756400000,-88.0970000000,09/03/2024," Collected by Fall 2024 students.<\/p>\n",Formicidae,DNeasy (Qiagen) blood and tissue kit.,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Team-4C.jpg,","",Yes,TATCGTTGGTTGATTCAGAATCGGGTCTCCCCTCCTGAAGGGATCAAAAAATGAAGTATTTAAGTTTCGATCAGTTAATAATATTCGTAATAGCTCCAGCTAAAACGGGAAGGGATAATAAAAGTAAAATTCGCAGTGATTAAGATAGATCATACAAGTAGGGGAATTTTATCAATAGAAAAATTTTTATGATGTATATTAAGGATTGTTGAAATAAAATTAATAGCTCCTAAAATAGATGATATACCGGCAATATGAAGAGAAAAAATTGTTAAGTCTACAGAAGGCCCATTATGAAAAATATTTGAAGCTAAAGGAGGGTAGATAGTTCACCCAGTTCCAGTACCATCATTAATAAAGTTTCTTAAAAGTAATAGAGTGATGGAAGGAGGTAAAAGTCAAAATCTTATATTATTTATACGAGGATAAGCTATATCTGGCGATCCTAATATTAAAGGAATTAGAAAATTTCCAAATCCACCAATTATAAATGGTATTACTATAAAAAAGATTATAATAAAGGCATGATTAGTTACTAAAGAATTATAAATTTGATCATTATTAATTAATGAATTTGAAGAACCTAATTCTAATCGAATAATTATACTTATAGAAGATCCAATTATTCCAGCTCAGATAGCAAATAAAAAATAAAGAATTCCAATATCTTTATGTT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Insect_MKAnthropod.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hemiptera-Varsha-Ayyala-12-March-1.jpg,",38.86270297268756,-77.11699497314451,," Freshwater stream in a forested area in the backyard of a house and scooped\u00a0up from the stream with a net.<\/span><\/p>\n",Decapoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImageVvAeg6-scaled.jpg,",37.2451640000,-78.4529740000,10/31/2024,"",Chauliognathus pensylvanicus,promega wizard SV,,Standard electrophoresis system,0.25 TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Slide1.jpeg,","A 1% agarose at 110V for 30 minutes, all well contained<\/span>10 \u03bcL<\/span>.<\/span> 1) ESO arthropod (CO1) PCR reaction, 2)\u00a0 ESO <\/span>Wolbachia<\/span><\/i> (W) PCR reaction, 3) Kendra CO1 PCR reaction, 4) Kendra W PCR reaction, 5) Saad CO1 PCR reaction, 6) Saad W PCR reaction, 7) Kassidy CO1 PCR\u00a0 reaction 8) Kassidy W PCR reaction 9) Positive control from both groups 10) 100bp NEB Ladder.\u00a0<\/span><\/p>\n
<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Pure-green-sweat-bee-scaled.jpg,",37.2516400000,-78.4529740000,10/31/2024,"
It was caught at a wildflower field near a lake in the Fall.<\/p>\n",Augochlora pura,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-28-at-9.20.47 AM.png,","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/processed-7DBDD935-BB8E-4300-8152-F986DF1D15C3.jpeg,",37.245164,-78.452974,10/31/2024," Wildflowers near Lake Mayes at Hampden-Sydney Campus<\/p>\n Season: Fall<\/p>\n Temperature: 78\u00b0F<\/p>\n Habitat: Terrestrial<\/p>\n",Atalopedes campestris,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screen-Shot-2025-04-14-at-9.32.34-AM.png,"," DNA extraction<\/strong>: The abdominal contents were removed and crushed thoroughly.<\/p>\nGel electrophoresis lanes for CO1 arthropod and Wolbachia<\/em> 16S rRNA PCR<\/strong>:<\/p>\n1: 1000bp ladder<\/p>\n 2-7: other people’s DNA<\/p>\n 8: arthropod primers<\/p>\n 9:\u00a0Wolbachia <\/em>primers<\/p>\n10: control DNA<\/p>\n All of the controls worked.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Carpender-Bee-scaled.jpg,",37.2451640000,-78.4529540000,10/31/2024," Large black and yellow bee with a large abdomen and thorax. Closely resemble a bumblebee with yellow and black markings and hind legs.<\/p>\n",Hymenoptera,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Wolbachia-Ladder.jpg,"," Wolbachia was not found in the arthropod sample. There was a PCR inhibitor that resulted in failure for DNA to amplify in PCR which is seen through no bands appearing for the arthropod sample.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/A83BE4A8-FFC3-4C77-A9D3-84568B8F8547-scaled.jpeg,",37.245164,-78.452954,10/31/2024,"",Orthoptera,DNeasy (Qiagen),Abdomen,MiniPCR,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-14-at-9.34.14-AM.png,"," DNA extraction: I made sure to crush the arthropod well, I dissected out the abdomen and excluded the exoskeleton.<\/p>\n Minor issues: During the DNA extraction I had to spin down my sample multiple times due to the consistency of the extracted DNA, the washes were not working and we were eventually able to spin it down and wash it after multiple attempts.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3200-scaled.jpeg,",37.245164,-78.452874,10/31/2024," The collection of samples took place in the fall when the weather was cooler. The terrestrial habitat (a lake) allowed for a good specimen collection.\u00a0<\/span><\/p>\n",Apis mellifera,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Slide1.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/ArthropodPhylogeny.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImagehGbw0e-scaled.jpg,",37.24212321878305,-78.45578638001656,10/31/2024,"Found in the fall in the wildflowers near lake Mayes, a terrestrial habitat at a temperature of 78\u00baF<\/p>\n",Atalopedes campestris,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Slide1-1.jpg,"," DNA extraction: I made sure to crush the arthropod really well dissecting the abdomen and not the exoskeleton.<\/p>\n Gel Electrophoresis Lanes for CO1 Arthropod:<\/p>\n 1- 1000bp ladder<\/p>\n 2- Arthropod positive PCR Product<\/p>\n 3- Wolbachia negative PCR Product<\/p>\n 10- Control DNA<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0927.jpg,",37.2451640000,-78.4529740000,12/13/2030,"",,DNeasy (Qiagen),Abdomen,MiniPCR,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot_28-4-2025_93059_docs.google.com_.jpeg,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0322-scaled.jpeg,",37.2451640000,-78.4529740000,10/31/2024,"",Enallagma civile,promega wizard SV,body without wings,Standard electrophoresis system,TAE,Ethidium Bromide,""," The whole body (minus wings) was used and this caused the filter to get clogged, and did not allow the DNA to amplify.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/huronsachem.jpg,",37.2451640000,-78.4629740000,10/31/2024," Fall<\/p>\n 78 Degrees Fahrenheit<\/p>\n Found in wildflowers near Lake Mayes<\/p>\n",Atalopedes huron,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/wolbachiagel.png,","",No,ATTAGGAACTTCTTTAAGATTATTAATTCGAACAGAACTAGGTAATCCTGGATCTTTAATTGGAGATGATCAAATTTATAATACTATCGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGATTTGGAAATTGATTAGTACCATTAATATTAGGAGCCCCTGATATAGCTTTCCCACGAATAAATAACATAAGATTTTGAATATTACCTCCTTCATTAACATTATTAATTTCAAGAAGAATTGTAGAAAATGGAGCAGGAACAGGATGAACTGTTTATCCTCCTTTATCCTCAAACATTGCTCATCAAGGATCTTCTGTTGATTTAGCTATTTTTTCTCTTCATTTAGCTGGAATTTCATCTATTTTAGGAGCTATTAATTTTATTACAACAATCATTAATATACGAATTAAAAATTTATCATTTGATCAAATACCTTTATTTGTATGATCTGTAGGAATTACAGCATTACTATTATTGTTATCTTTACCTGTATTAGCAGGAGCTATTACTATATTACTTACAGATCGAAATT,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_8361-scaled.jpeg,",37.34385169999999,-78.4069732,10/31/2024,"",Palpada vinetorum,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Gel2-Figure.jpg,","",No,NNNNNNGNNTTTGGTACNTGAGCAGGTATAGTAGGAACTTCATTAAGAATTTTAATTCGAGCTGAACTAGGTCATCCAGGTGCATTAATTGGTGATGATCAAATTTATAATGTTATTGTAACAGCTCATGCTTTTGTAATAATTTTCTTTATAGTAATACCTATTATAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCTCCTGATATAGCATTCCCTCGAATAAATAATATAAGTTTTTGATTATTACCTCCTTCTTTAACTTTATTATTAGTAAGAAGTATAGTAGAAAATGGAGCTGGAACAGGATGAACAGTTTATCCTCCTTTATCAAGTAATATTGCTCATGGAGGAGCTTCAGTTGATTTAGCAATTTTCTCACTTCATTTATCTGGTATATCATCAATTTTAGGGGCTGTAAATTTTATTACAACAGTTATTAATATACGATCTACAGGTATTACATATGATCGAATACCTTTATTTGTTTGATCTGTTGCTATTACTGCATTATTATTATTACTTTCATTACCAGTATTAGCTGGAGCTATTACTATATTATTAACAGATCGAAATTTAAATACAACATTTTTTGATCCTGCAGGTGGTGGAGATCCAATTTTATACCAACATTTATTTTGATTTTTTGNNCNNCNNNNAANTTTAAN,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/asian-lady-beetle-pic.png,",37.2426032,-78.4605072,10/31/2024,"",Harmonia axyridis,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Wolbachia-project-gel.png,"," The KOCO1 well is positive for CO1 gene<\/p>\n The KOW well is negative for Wolbachia<\/p>\n The control DNA well is the positive control for CO1 gene and 16s rRNA gene<\/p>\n The last well is the 1000 bp DNA ladder<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6519-scaled.jpeg,",37.2451640000,-78.4529740000,10/31/2025," The arthropod was collected on fall day with a temperature of 78\u00ba F. Found near a lake with flowers.<\/p>\n",Ischnura posita,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-14-at-9.39.28 AM.png,"," DNA extraction: I made sure to crush the abdomen well. I dissected the abdomen out and made sure to not include the exoskeleton.<\/p>\n Gel Electrophoresis lanes for CO1 arthropod PCR.<\/p>\n 1.100bp Ladder<\/p>\n 8. The tenth well was my arthropod sample.<\/p>\n 9. The ninth well was my wolbachia sample.<\/p>\n 10. The tenth well contained the positive and negative PCR controls.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5081-scaled.jpeg,",37.245164,-78.452974,10/31/2024," The arthropod was caught near wildflowers Lake Mayes. It has large eyes and abdomen with large wings that extend past the abdomen. It was mostly black with a little yellow on the thorax.<\/p>\n",Xylocopa,DNeasy (Qiagen),Abdomen,,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BIOL-324-Arthropod-Gel.jpg,"," DNA extraction: The arthropod was cut at the abdomen and the contents were grounded with a pestle thoroughly.<\/p>\n Loading Gel Lanes:<\/p>\n lane 1- 100bp DNA ladder<\/p>\n lane 2-7- other group members’ samples<\/p>\n lane 8-9- my arthropod and Wolbachia<\/em> samples<\/p>\nlane 10- DNA positive control<\/p>\n <\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/eastern-tailed-blue.jpg,",37.245164,-78.452974,10/31/2024," Location: Wildflower field near Lake Mayes<\/p>\n Season: Fall<\/p>\n Temperature: 78 degrees F<\/p>\n Habitat: Terrestrial<\/p>\n",Lycaenidae,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Gel-5-with-labeling.png,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Short-horned-grasshoppper-image-scaled.jpg,",37.245164,-78.452974,10/31/2024,"",Melanoplus,promega wizard SV,Abdomen,Standard electrophoresis system,0.25 TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-14-at-9.35.15-AM.png,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_1097.jpg,",37.3011371773,-78.3985835871,10/31/2024," Fall, 78 deg F<\/p>\n",Scolia dubia,Wizard SV (Promega),Partial abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Slide2.jpg,"," In a 1.5 mL microfuge tube, the specimen and 275 \u00b5L of prepared Digestion Solution Master Mix, containing proteinase K, RNase A Solution, and 0.5 M EDTA. A pestle was used to physically grind the specimen and placed in a 55 \u2103 water bath for 15 minutes for cell lysis. The sample was centrifuged for 60 seconds at 2000 x <\/span>g <\/span><\/i>and transferred to a new 1.5 mL microfuge tube with 250 \u00b5L of Wizard SV Lysis Buffer. The lysate sample was centrifuged for 3 minutes at 13,000 x <\/span>g. <\/span><\/i>The column was washed with 650 \u00b5L of Wizard SV Wash and centrifuged for 60 seconds at 13,000 x <\/span>g. <\/span><\/i>This wash process was repeated four times and 50 \u00b5L of 65 \u2103 nuclease-free water was added. The eluted samples were stored at -20 \u2103.\u00a0<\/span><\/p>\nPolymerase Chain Reaction<\/b><\/p>\n The Arthropod PCR Cocktail was prepared in a 1.5 mL microcentrifuge tube with adequate measurements (Table 2), cortexed and centrifuged for proper mixing. Two 0.2 mL PCR tubes were tabled with control and extracted specimen DNA. In each 0.2 mL tube, 23 \u00b5L of the Arthropod PCR Cocktail was added and centrifuged. In the corresponding labeled PCR tubes, 2 \u00b5L of control and extracted specimen DNA was added. The samples were transferred to a BioRad Thermal Cycler and set for 2 minutes at 94<\/span>\u00b0C for initial denaturation, 30 cycles at 30:45:60 second intervals at 94:49:72\u00b0C respectively, final extension was for 10 minutes at 72\u00b0C and held at 4\u00b0C. The 0.2 mL PCR tubes were stored in <\/span>-20 \u2103.\u00a0<\/span><\/p>\n <\/p>\n \n\n\nTable 2.<\/b>\u00a0<\/span><\/p>\n Arthropod PCR Cocktail<\/span><\/td>\n | Chemical\u00a0<\/span><\/td>\n | Volume<\/span><\/td>\n<\/tr>\n\n<\/td>\n | Arthropod Primer F\/ R<\/span><\/td>\n | 5 \u00b5L<\/span><\/td>\n<\/tr>\n\n<\/td>\n | Water<\/span><\/td>\n | 16.25 \u00b5L<\/span><\/td>\n<\/tr>\n\n<\/td>\n | Taq Master Mix, 2X<\/span><\/td>\n | 31.25 \u00b5L<\/span><\/td>\n<\/tr>\n\nWolbachia PCR\u00a0<\/span><\/td>\n | <\/td>\n | <\/td>\n<\/tr>\n | \n<\/td>\n | Wolbachia Primer F\/ R<\/span><\/td>\n | 5 \u00b5L<\/span><\/td>\n<\/tr>\n\n<\/td>\n | Water<\/span><\/td>\n | 16.25 \u00b5L<\/span><\/td>\n<\/tr>\n\n<\/td>\n | Taq Master Mix, 2X<\/span><\/td>\n | 31.25 \u00b5L<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n <\/p>\n The 16s rRNA <\/span>Wolbachia <\/span><\/i>primer was prepared in a 1.5 mL PCR tube with the adequate measurements (Table 2). Two 0.2 mL PCR tubes were tabled with control and extracted specimen DNA. In each 0.2 mL tube, 23 \u00b5L of the <\/span>Wolbachia <\/span><\/i>primer mix was added and centrifuged. In the corresponding labeled PCR tubes, 2 \u00b5L of control and extracted specimen DNA was added. The samples were transferred to a BioRad Thermal Cycler and set for 2 minutes at 94<\/span>\u00b0C for initial denaturation, 30 cycles at 30:45:60 second intervals at 94:55:72\u00b0C respectively, final extension was for 10 minutes at 72\u00b0C and held at 4\u00b0C. The 0.2 mL PCR tubes were stored in <\/span>-20 \u2103.\u00a0<\/span><\/p>\n | | | | | | | |