14 legs (7 pairs), oval shape, hard outer shell<\/p>\n",Isopoda,,,,,,"","",,,,, "",,,07/11/2022,"",Isopoda,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/07/IMG_20220711_105504.jpg,",39.4227,77.4188,07/11/2022,"
Brown Small<\/p>\n",Isopoda,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/07/16575510673241286739112.jpg,",,,07/11/2022,"",Armadillidium vulgare,,,,,,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/07/Taufliege_Drosophila_melanogaster-20200607-RM-210232.jpg,",36.14784828820093,-86.80251734193155,01/01/2021,"
This is the positive control of Vanderbilt University<\/p>\n",Drosophila melanogaster,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/07/bBK22-BK28.JPG.png,","",Yes,AACATCTTTAAGAATTTTAATTCGAGCTGAATTAGGACATCCTGGAGCATTAATTGGAGATGATCAAATTTATAATGTAATTGTAACTGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGTGGATTTGGAAATTGATTAGTGCCTTTAATATTAGGTGCTCCTGATATAGCATTCCCACGAATAAATAATATAAGATTTTGACTTCTACCTCCTGCTCTTTCTTTACTATTAGTAAGTAGAATAGTTGAAAATGGAGCTGGGACAGGATGAACTGTTTATCCACCTCTATCCGCTGGAATTGCTCATGGTGGAGCTTCAGTTGATTTAGCTATTTTTTTCTCT,https://wolbachiaprojectdb.org/wp-content/uploads/2022/07/BK26CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK28.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Formica rufa,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK22-BK28.JPG.png,","",No,TAATTGGTTCATCTATAAGAATAATTATTCGACTAGAATTAGGTTCTTCTAATTCATTAATTAATAATGATCAAATTTATAATTCTATAGTTACAAGACATGCATTTGTTATAATTTTCTTTATAGTTATGCCTTTCATAATTGGAGGATTTGGAAATTTTCTAGTACCTTTAATATTAGGATCACCTGATATAGCATACCCCCGTATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATTTCTCTCCTTCTTTTAAGAAATTTTATTAATGACGGAGTAGGAACAGGATGAACTGTTTATCCTCCATTAGCTTCTAATATTTTCCATAATGGCCCCTCAGTTGATTTAACTATTTTTTCTCTTCATATTGCTGGTATATCCTCTATTCTAGGAGCTATCAATTTTATTTCAACTATTATAAATATACATCATAAAAATTTTTCTATCGATAAAATTCCCTTACTTGTATGATCAATTTTAATCACTGCAATTTTATTACTTTTATCACTTCCAGTTCTTGCAGGAGCTATCACTATACTTCTAACTGACCGTAACCTTAATACTTCATTTTTTGACCCATCTGGTGGTGGTGATCCTATTTTATATCAACATCTCTTCTG,https://wolbachiaprojectdb.org/wp-content/uploads/2022/07/BK28CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK27.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Calosoma inquisitor,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK22-BK28.JPG.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK25A.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK25B.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Hylotrupes bajulus,DNeasy (Qiagen),Larva,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK22-BK28.JPG.png,","
We cut the organism lengthwise in 4 parts from head to end and take the third quarter<\/p>\n",No,CAGGAATAGTTGGRACATCCTTAAGATTGTTAATTCGAACAGAACTAGGAAATCCAGGATCTTTAATTGGAGATGATCAAATCTATAATGTTATTGTTACTGCCCATGCTTTTATTATAATTTTTTTCATAGTTATACCAATTATAATTGGAGGATTTGGAAACTGATTAGTTCCCCTAATATTGGGGGCTCCCGATATAGCTTTTCCTCGAATAAATAATATAAGATTTTGACTTTTACCTCCTTCTTTAACTTTATTAATCATAAGAAGAGTGGTAGAAAATGGGGCTGGTACCGGATGAACCGTCTATCCCCCTCTTTCTTCAAATATTGCTCATAGAGGATCTTCAGTTGATTTAGCAATTTTTAGGTTACATTTAGCAGGTATCTCTTCAATTCTTGGTGCAGTAAATTTTATCACTACAGTAATTAATATACGACCAATTGGAATAACTCCTGATCGTGTTCCTTTATTTGTTTGGGCAGTAGTAATTACTACAATTCTTCTTCTTCTTTCATTACCAGTTTTAGCTGGAGCAATTACTATACTTTTAACAGATCGAAATTTAAATACTTCATTTTTTGATCCTGCAGGAGGTGGTGATCCTATTCTTTATCAACATTTATTTTGATTTTTTGTCACAC,https://wolbachiaprojectdb.org/wp-content/uploads/2022/07/BK25CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK24.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Porcellio scaber,DNeasy (Qiagen),Rear half,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK22-BK28.JPG.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK23A.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK23B.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Phosphuga atrata,DNeasy (Qiagen),Gonads dissected,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK22-BK28.JPG.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK22.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Lithobius forficatus,DNeasy (Qiagen),Rear half,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK22-BK28.JPG.png,","",No,ATTAAGCCAACCAGGGAGACTTATTGGAGATGACCAAATTWATAATGTAATCGTAACAGCCCACGCATTCATTATAATCTTCTTTATAGTAATACCTATCATAATTGGAGGATTCGGTAATTGATTGGTGCCCTTAATACTAGGGGCACCAGACATAGCTTTCCCACGATTAAACAATATAAGATTTTGACTATTGCCGCCCTCACTAACGTTACTTCTATCCTCAGCTGCCGTAGAAAGAGGGGCAGGAACAGGATGAACAGTCTACCCCCCCTTGTCTTCTAATATCTCCCATAGTGGAGCATCAGTTGATATAACAATTTTCTCTCTTCATTTAGCAGGTGCATCCTCAATTCTAGGGGCAATCAATTTCATTTCAACAATCATTAACATACGATCAAGAGGAATAACATTTGAGCGAGTACCATTATTCGTATGATCTGTAAAAATCACAGTAATTCTTCTATTATTATCTTTGCCCGTATTAGCAGGAGCAATCACAATACTATTAACAGATCGAAACTTAAATACAAGCTTCTTTGACCCAACAGGAGGAGGAGACCCCATCCTATATCAACATCTATTCTGATTTTTGTMAGAAAAAAAGAGAGAAAAAAAAAAAAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2022/07/BK22CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK21.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Chelidurella acanthopygia,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK15-BK21.JPG.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK20.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Limnephilidae,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK15-BK21.JPG.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK19.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Figitidae,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK15-BK21.JPG.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK18.jpg,",47.459168434,9.0190836469,04/30/2021,"",Lycosidae,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK15-BK21.JPG.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK17.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Dolichopus,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK15-BK21.JPG.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK16.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Acanthosoma haemorrhoidale,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK15-BK21.JPG.png,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK15.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Himacerus mirmicoides,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK15-BK21.JPG.png,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK14.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Scaeva pyrastri,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK08-BK14.JPG.png,","",No,TTCATTAAGAGTTTTAATTCGTGCAGAACTTGGACATCCAGGAGCTTTAATTGGAGATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTGTTATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGAAATTGATTAGTTCCATTAATATTAGGAGCTCCTGATATAGCATTCCCTCGAATAAATAATATAAGTTTTTGATTATTACCTCCTTCTTTAACATTATTATTAGTAAGAAGAATAGTTGAAAATGGAGCAGGAACTGGTTGAACAGTTTACCCTCCTCTTTCTGCTAGAATTGCTCATGGAGGAGCCTCTGTTGATTTAGCTATTTTTTCTCTACATTTAGCTGG,https://wolbachiaprojectdb.org/wp-content/uploads/2022/07/BK14CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK13.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Tachina intricata,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK08-BK14.JPG.png,","",Yes,ACATCATTAAGTGTATTAATTCGTGCAGAACTTGGTCATCCTGGTGCTTTAATTGGAGATGATCAAATTTATAATGTAATTGTTACAGCCCATGCTTTTGTAATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGTAATTGATTAGTTCCATTAATATTAGGAGCTCCTGATATAGCATTTCCTCGTTTAAATAATATAAGTTTTTGACTATTACCTCCTTCTTTAACATTATTATTAGTAAGTAG,https://wolbachiaprojectdb.org/wp-content/uploads/2022/07/BK13CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK12.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Trichocera hiemalis,DNeasy (Qiagen),body without wings,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK08-BK14.JPG.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK11.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Leptothorax tuberum,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK08-BK14.JPG.png,","",No,AATTGGCTCATCTATAGAATAATCATTCGACTAGAATTAGGTTCTTCTAATTCATTAATTAATAATGATCAAATTTATAATTCTATAGTTACAAGACATGCATTCGTTATAATTTTCTTTATAGTTATGCCTTTCATAATTGGAGGATTTGGAAATTTTCTCGTACCTTTAATATTAGGATCACCTGATATAGCATACCCCCGTATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATTTCTCTCCTTCTTTTAAGAAATTTTATTAATGACGGAGTAGGAACAGGATGAACTGTTTATCCTCCATTAGCTTCTAATATTTTTCATAATGGTCCCTCAGTTGATTTAACTATTTTTTCTCTTCATATTGCTGGAATATCTTCTATTCTAGGAGCTATCAATTTTATTTCAACTATTATAAATATACATCATAAAAATTTTTCTATCGATAAAATTCCCTTACTTGTATGATCAATTTTAATCACTGCAATTTTATTACTTTTATCCCTCCCAGTTCTTGCAGGAGCTATCACTATACTTCTAACTGACCGTAACCTTAATACTTCATTTTTTGACCCATCTGGTGGTGGTGATCCTATTTTATACCAACATCTCTTCTGATTTTTGCACCCCAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2022/07/BK11CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK10.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Alopecosa accentuata,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK08-BK14.JPG.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK09.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Myrmica scabrinodis,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK08-BK14.JPG.png,","",Yes,GTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCNACAATGGTGGCTACNATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGT,https://wolbachiaprojectdb.org/wp-content/uploads/2022/07/BK09CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK08.jpg,",47.459168434,9.0190836469,04/30/2021,"",Mycetophilidae,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK08-BK14.JPG.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK07.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Psylliodes cucullata,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK01-BK07.JPG.png,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK06.jpg,",,,04/30/2021,"",Porcellio scaber,DNeasy (Qiagen),Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK01-BK07.JPG.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK05.jpg,",47.4591684340,9.0190836469,04/30/2021,"",Tenebrio,DNeasy (Qiagen),Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK01-BK07.JPG.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK04-1.jpg,",47.4591684349,9.0190836470,04/30/2021,"",Musca domestica,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK01-BK07.JPG.png,","",No,CCTCTCTAAGAATTTTAATTCGAGCAGAACTAGGACATCCTGGAGCATTAATTGGCGATGATCAAATTTATAATGTAATTGTAACAGCTCATGCCTTTGTTATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGAAATTGACTTGTCCCTTTAATACTAGGAGCACCTGATATAGCATTTCCTCGAATGAATAATATAAGTTTTTGACTTTTACCTCCTGCACTTACACTACTATTGGTGAGCAGTATAGTGGAAAACGGAGCTGGGACAGGATGAACTGTTTACCCTCCTCTATCTTCTAATATTGCTCATGGAGGAGCTTCTGTTGATTTAGCTATCTTTTCCCTTCATTTAGCAGGAATTTCTTCAATTTTAGGAGCAGTTAATTTTATTACAACTGTTATTAATATGCGATCTACAGGAATTACATTTGACCGAATACCTTTATTTGTTTGATCTGTAGTAATTACAGCATTATTACTCCTTTTGTCCTTACCTGTATAGCAGGAGCTATTACTATATTATTAACAGATCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGAGGAGGTGA,https://wolbachiaprojectdb.org/wp-content/uploads/2022/07/BK04CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK03.jpg,",47.4599479775,9.0185277149,04/30/2021,"",Agriotes,DNeasy (Qiagen),Other,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK01-BK07.JPG.png,","
We cut the organism lengthwise in 4 parts from head to end and take the third quarter<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK02-2.jpg,",47.4589363085,9.0186894628,04/30/2021,"",Tachypodoiulus niger,DNeasy (Qiagen),Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK01-BK07.JPG.png,","
We cut the organism lengthwise in 4 parts from head to end and take the third quarter<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/20220606_111151-scaled.jpg,",40.6201951000,-74.5712657000,06/06/2022,"
Has 6 legs<\/p>\n
Dark Color Body<\/p>\n
Red Eyes<\/p>\n
2 Wings<\/p>\n
Caught in the woods bye the lunch tables<\/p>\n",Sarcophaga sexpunctata,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/Nikita-Gel.png,","
Ran without controls<\/p>\n",Yes,GATGACCAAATTTATAATGTAATTGTAACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCAATTATAATTGGAGGATTTGGTAATTGATTAGTTCCTCTTATACTAGGAGCTCCTGATATAGCTTTCCCTCGAATAAACAATATAAGATTTTGACTTCTTCCTCCTGCACTAACTTTACTATTAATAAATACATTAATAGAAAATGGAGCTGGAACAGGATGAACTGTTTATCCTCCCTTATCATCTAATATTGCTCATAGAGGACCTTCTGTAGATTTAACTATTTTTTCTCTTCATTTACTGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACAACAACTATTAATATGCGTTCAACAGGTATTACTTTTGATCGAATACCTTTATTTGTTTGATCAGTATTATTACAGTATTCTTTTATTACTTTCTTTACCAGTTCTTGCGGACAATTACTATATTATTAACTGATCGAAATATAAATACTTCCTTTTTTGACCCACAGGAGGAGGA,https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/MD13-Premixed.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/close_up_bug.jpg,",40.6200974212,-74.5683027804,08/06/2022,"
Collected outside Pingry senior area.<\/p>\n",Hemiptera,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/Untitled-presentation-1.jpg,","
All bands appeared in expected locations. As this was our second time completing the protocol, we ran all experiments without controls.<\/p>\n",Yes,CAATAAGAAGAATAATTATTCGATTAGAATTAATACAACCAGGATCAATAATTAAAAATGACCAAATCTATAATACCATCGTAACATCTCATGCATTCATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAACTGATTAGTTCCTATAATAATCGGTGCACCAGACATGGCATTCCCACGAATAAATAATATAAGATTTTGATTACTACCACCATCAATTTCACTTTTAATTGCAAGATCAATCGTAGGGTCAGGATCAGGTACAGGATGAACTGTTTACCCCCCTCTCTCAGCCCAAATTGCACATTCAGGACCTTCAGTAGACCTAACTATTTTTTCACTTCACATCGCAGGTTTAAGATCAATCATAGGAGCAATCAATTTCATTTCAACAACAATAAATATACGGCCAAAAGGAATAACTATAGAAAAAATACCCCTTTTCTGTTGATCAGTCCTAATTACAGCAGTTTTACTACTAGTATCATTACCAGTACTAGCTGGAGCAATTACAATACTACTAACAGATCGAAACTTTAATACCTCATTTTTTGACCCCACGGGTGGAGGAGACCCAATTTTATATCAACACTTATTCTGATTTTTTGGT,https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/21-final.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/ant1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/ant2-scaled.jpg,",40.621685,-74.56,06/06/2022,"
<\/p>\n
Ant specimen was found in a terrestrial environment – decomposing leaves next to a decomposing log within The Pingry School’s forest. It was caught at the beginning of June 2022 (start of Summer\/end of Spring), around noon. Temperature was 23.9 degrees celsius. Attached is a picture of the place I found the Ant in (with the ant in the picture as well).<\/p>\n",Camponotus chromaiodes,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/Screen-Shot-2022-06-09-at-1.43.33-PM.png,","
The gel contains me and my lab partner’s DNA – my Insect and Wolbachia DNA are in lanes 4 and 5 respectively.<\/p>\n
Lane 1 – DNA Ladder<\/p>\n
Lane 2 – Partner’s ant<\/p>\n
Lane 3 – Partner’s wolbachia test<\/p>\n
Lane 4 – My Ant Test<\/p>\n
Lane 5 – My Wolbachia test<\/p>\n
Analysis:<\/p>\n
The expected fragment for both Wolbachia 16S and Arthropod CO1 both showed up, in lanes 5 and 4 respectively. Wolbachia 16S has a base pair length of 438 base pairs, and in my gel’s lane 5 there is a band under the 500 base pair DNA ladder band – indicating the expected fragment. Arthropod CO1 has a base pair length of 708 base pairs, and in lane 4, there is a band between the 1000 base pair and 500 base pair length bands – indicating the expected fragment. DNA Sequencing further supported the identification of Carpenter Ant and + Wolbachia DNA, as when I input my sequences into BLAST, we observed very strong matches for both Wolbachia and Carpenter ant DNA.<\/p>\n",Yes,TGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTGGGATCTCCTGATTCACTAATTCTTAATGATC AAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTT GGTAATTTTTTAATTCCCCTTATACTAGGATCTCCTGATATAGCTTACCCCCGTTTAAATAACATAAGATTTTGATTACT TCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGGACTGTCTACCCCCCTC TATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATT ATAGGAGCAATCAATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTAGT ATGGTCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGAGCTATTACAATACTACTAACAG ACCGAAATCTTAATACTTCATTTTTC,https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/MD17-Premixed-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/IMG_6773-2-scaled.jpg,",40.6199394030,-74.5419507900,09/27/2021,"
- \n
- Red and brown wings with black spots.<\/li>\n
- Colored brown, black, white, and red.<\/li>\n
- Found in fall 2021 and frozen at -80\u00baC<\/li>\n<\/ul>\n",Lycorma delicatula,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/Screen-Shot-2022-06-09-at-1.22.16-PM.png,","",Yes,TCCTCTATAAGAATAATCATTCGACTAGAGTTGGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCAATACCAT CGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTAATTC CCCTTATACTAGGATCTCCTGATATAGCTTACCCCCGTTTAAATAACATAAGATTTTGATTACTTCCTCCATCGATCTCC TTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGGACTGTCTACCCCCCTCTATCATCAAATACCTT CCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATCAATT TTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTAGTATGGTCTATTCTTATT ACAGCTATTCTCCTTCTTCTATCCCTACCTGTTCTAGCAGGAGCTATTACNATACTACTAACAGACCGAANTCTTAATAC TTCATTTTTCGACCCTTCGGGAGGGGGAGATCCTATTTTATACCA,https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/MD07-Premixed.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/Screen-Shot-2022-06-09-at-1.03.26-PM.png,",40.7066174000,-74.5493284000,09/27/2021,"
spotted wings, orange-red wings, found by trees in a grassy area in fall 2021 and frozen at -80 degrees Celsius<\/p>\n",Hemiptera,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/Screen-Shot-2022-06-09-at-1.09.14-PM.png,","
Lane 1 – Ladder<\/span><\/p>\n
Lane 2 – Lycorma delicatula<\/em> Arthropod 1x dilution (COI)<\/span><\/p>\n
Lane 3 – Lycorma delicatula<\/em> Arthropod 10x dilution (COI)<\/span><\/p>\n
Lane 4 – Lycorma delicatula<\/em> Wolbachia 1x dilution (16s)<\/span><\/p>\n
Lane 5 – Lycorma delicatula<\/em> Wolbachia 10x dilution (16s)<\/span><\/p>\n
Band in lane two at 708 bp shows that the Lycorma delicatula<\/em> is an arthropod and the band in lane four at 438 bp shows the Lycorma delicatula<\/em> has Wolbachia. Other lanes are other dilutions and a different bug and the protocol ran without controls.<\/p>\n",Yes,CGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCCAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGT,https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/MD09-Premixed-final-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/IMG_4876-1-scaled.jpg,",40.6201834252,-74.5664760435,09/27/2021,"
Lanternfly has black spots on its wings (looks like ink spatters); ends of wings have small, black pinpricks on its wings; folded black legs; black body; abdomen is shiny and black-and-white striped; underside of wings is darkish but bright red; small, black head<\/p>\n
The lanterfly was found by another student (not myself) outside of my school doors on the pavement. The bug was found in early fall and the temperature was 21.1\u00ba C.<\/p>\n",Lycorma delicatula,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/Screen-Shot-2022-06-09-at-12.40.21-PM.png,","
DNA extraction<\/strong>: I dissected the lanternfly’s abdomen, extracted its dna, and macerated it (excluding exoskeleton). Unfortunately, I did not macerate the abdomen extract for long enough in the cell lysis buffer, resulting in a lower concentration of DNA.<\/p>\n
Gel<\/strong> electrophoresis<\/strong> lanes are as follows:<\/p>\n
- \n
- DNA ladder 1<\/li>\n
- My lab partner’s lanternfly tested for C01 arthropod gene<\/li>\n
- My lanternfly tested for C01 arthropod gene<\/li>\n
- break<\/li>\n
- My lab partner’s lanternfly tested for wolbachia gene<\/li>\n
- My lanternfly tested for wolbachia gene<\/li>\n
- DNA ladder 2<\/li>\n<\/ol>\n
Analysis<\/strong>: No controls were used in this experiment due time restrictions. Faint bands are present at the arthropod and wolbachia bp location.<\/p>\n
Sequencing<\/strong>: Both arthropod and wolbachia samples from my bug were sequenced, but both had very low quality (overall Q value was 19). Nonetheless, when I “blastn” and “blastx” the sequences, they matched to the corresponding arthropod and a wolbachia dna.<\/p>\n",Yes,NNNNNNNNNTNNNTNNNGGGTATGATGNNNACTANTNGGNACGNNAGAAGAATAATNATTCGAATAGAATTANNACAANC AGGATCAATTATTAGGAATGACCAAATCTATAAAACCATCGGTACCTCTCCTGGATTCATTATTGATTTCTTTATAGNNA TACCTATTATAATTGGAGGATTTGGAAACTGATTAGTTCCTATAATTTTTGGGGCCCCCTACATGGCATTCCCACGAATA AATAATATAANATTTTGATTACTACCNCCATCAATTTCACTTTTAATTGCAAGATCAATCGGAGGGACAGGATCAGGTAC AGGATGAACTGTTTACCCCCCTCTCTCCCCCCAAATTGCCCTTTCAGGACCTTCANTAGACCTAACTATTTTTTCACTTC NCTTCGCAGGTTTAAGATCANTCNTAGGAGCAATCAATTTCATTTCNACAACAATAAATATACGGCCAAAAGGAATAACT ATAGAAAAAATACCCCTTTTCTGTTGATCANTCCTAATTACAGCAGATTTACTACTAGTATCATTACCAGTACTAGCTGG AGCAATTACAATACTACTAACAGATCNAAACTTNAANACCNCNTTTTTTGACCCCNCGGNNGGAGGAGANCNNNTTTTAT ATCAACACTTATTCTGATTTTTTGGTCNCCCNGAAGATNAANAA,https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/MD05-Premixed.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/Sphecomyia-vittata.png,",40.6202000000,-74.5677000000,06/06/2022,"
The arthropod specimen was collected from a grass habitat directly adjacent a forest and was observed nectaring on white clover blossoms.<\/p>\n",Sphecomyia vittata,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/Gel-Electrophoresis.png,","
The Gel Imaging System was programmed incorrectly, resulting in an image of inappropriately high contrast. This experiment was conducted without controls.<\/p>\n",Yes,CGGACTTGAGCTGGATAGTAGGAACATCCTTAAGAATTTTAATTCGTGCAGAATTAGGCCATCCTGGAGCATTAATTGGAGATGACCAAATTTATAATGTTATTGTTACTGCTCATGCTTTTGTTATAATTTTTTTTATAGTTATACCAATTATAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCCCCAGATATAGCCTTTCCTCGAATAAATAATATAAGTTTTTGATTACTACCCCCTTCTTTAACTTTATTATTAGTAAGAAGTATAGTAGAAAATGGAGCTGGAACGGGATGAACTGTTTATCCTCCTCTTTCTGCAAGAATTGCCCATGGAGGTGCTTCTGTTGATTTAGCAATTTTTTCTCTACATTTAGCAGGAATATCTTCTATTTTAGGAGCAGTAAATTTTATTACTACTGTTATTAATATACGGTCTACTGGAATTTCATATGATCGAATACCATTATTTGTATGATCAGTAGTAATTACAGCCCTATTACTTTTACTATCATTACCTGTATTAGCGGGAGCAATTACAATACTTTTAACTGATCGAAATTTAAATACCTCTTTTTTTGATCCAGCAGGTGGGGGAGATCCTATTCTTTACCAACATTTATTTTGA,https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/MD15.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/IMG_4830-1-scaled.jpg,",40.6202000000,-74.5677000000,05/26/2022,"
Black ant; about 5mm; 6 legs; redish colored legs and antennae; hairs on body; head, thorax, and abdomen are shiny black; fast runner. The common name for the ant is a black field ant.<\/p>\n
I found the ant in the “backyard” of a school at a clearing before the entrance to the woods. The ant was collected in late May, the temperature was 20.556\u00ba C, it was sunny, and the ant was crawling across a patch of dry dirt among some weeds and grass.<\/p>\n",Formica subsericea,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/Screen-Shot-2022-06-08-at-10.12.01-AM.png,","
DNA extraction<\/strong>: I simply mashed the entire bug to collect the abdomen DNA<\/p>\n
PCR<\/strong>: I made a mistake during PCR because I pipetted too much “PCR cocktail” into one of the PCR tubes (not sure which) and didn’t have enough cocktail for the Wolbachia control (it is omitted from the gel).<\/p>\n
Gel electrophoresis lanes<\/strong> for the image above:<\/p>\n
- \n
- DNA ladder 1<\/li>\n
- My ant tested for CO1 arthropod gene<\/li>\n
- My lab partner’s ant tested for CO1 arthropod gene<\/li>\n
- Positive arthropod control<\/li>\n
- Negative arthropod control<\/li>\n
- Blank (was supposed to be positive wolbachia dna control)<\/li>\n
- DNA water control<\/li>\n
- Break<\/li>\n
- My ant tested for wolbachia dna<\/li>\n
- My lab partner’s ant tested for wolbachia dna<\/li>\n
- Positive arthropod control<\/li>\n
- Negative arthropod control<\/li>\n
- Positive wolbachia control<\/li>\n
- Water control<\/li>\n
- DNA ladder 2<\/li>\n<\/ol>\n
Analysis<\/strong>:<\/p>\n
The ants had very strong bands, but the controls did not work as expected. Additionally, the wolbachia side of the gel has faint bands at the arthropod bp location.<\/p>\n
Sequencing<\/strong>: Unfortunately, my wolbachia sequencing sample dried up and was unable to be sequenced. Only the arthropod sample was sequenced, resulting in an average Q-value of 39. When I “blastn” and “blastx” the sequence, I got my type of arthropod.<\/p>\n",Yes,AATTAGGCTCATCTAATTCATTAATCAATAATGATCAAATTTATAATTCTTTAGTAACTAATCACGCTTTTATTATAATT TTTTTCATAGTAATACCATTTATAATTGGTGGATTTGGAAATTTTCTAATTCCTTTAATACTAGGATCACCAGACATAGC ATACCCTCGTATAAATAATATAAGATTTTGACTCTTACCTCCTTCAATTACTCTTTTACTTTTAAGAAATTTTATTAATG ACGGAACAGGAACAGGATGAACTATTTATCCTCCCTTATCCTCTAATATTTTTCATAATGGTCCTTCTGTAGACTTAACA ATTTTTTCTCTTCATATTGCAGGTATATCCTCAATTTTAGGAGCAATTAATTTTATTTCAACAATTCTTAATATACATCA TAAAAATTTTTCTATTGATAAAATTCCTTTGCTCGTATGATCAATTTTAATTACAGCTATCTTACTTCTATTATCCTTAC CTGTATTAGCCGGAGCTATTACTATACTATTAACTGATCGAAATTTAAATACTT,https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/MD11-Premixed.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/IMG_6694-2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/IMG_6672-2-scaled.jpg,",40.6199394032,-74.5419507935,05/26/2022,"
- \n
- Black ant<\/li>\n
- 6 legs<\/li>\n
- Fast runner<\/li>\n
- Slightly hairy<\/li>\n<\/ul>\n",Camponotus pennsylvanicus,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/Screen-Shot-2022-06-08-at-10.09.52-AM.png,","",Yes,TGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTGGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCAATA CCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTA ATTCCACTTATACTAGGATCTCCTGATATAGCTTACCCTCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATCGAT CTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGAACTATCTACCCCCCTCTATCATCAAATA CCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATC AATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCATTATTAGTATGATCTATTCT TATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGCGCTATTACAATACTACTAACAGACCGAAATCTTA ATACTTCATTTTTCGATCCCTCGGGAGGAGGAGATCCTATTTTATACCAACATTTATTTTGATTTTT,https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/MD09-Premixed.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/Screen-Shot-2022-06-08-at-9.22.43-AM.png,",40.7066174000,-74.5493284000,05/26/2022,"
near a dead tree, medium-sized, fast, found in the woods<\/p>\n",Hexapoda,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/Screen-Shot-2022-06-08-at-10.09.12-AM.png,","
lane 2 – Camponotus chromaiodes<\/em> arthropod (COI)<\/p>\n
lane 9 – Camponotus chromaiodes<\/em> Wolbachia (16s)<\/p>\n
The expected fragment for an arthropod is seen at 438 bp and the expected fragment for Wolbachia is seen at 708 bp.<\/p>\n",Yes,TCCTCTATAAGAATAATCATTCGACTAGAGTTGGGATCTCCTGATTCACTAATTCTTAATGATCGAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTAATTCCCCTTATACTAGGATCTCCTGATATAGCTTACCCCCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGGACTGTCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTAGTATGGTCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGAGCTATTACAATACTACTAACAGACCGAAATCTTAATACTTCATTTTTCGACCCTTCGGGAGGGGGAGATCC,https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/MD03-Premixed-6-8-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/BK01-2.jpg,",47.45990589673516,9.018233739459234,04/30/2021,"",Pyrrhocoris apterus,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/bBK01-BK07.JPG.png,","",No,,,, "",38.063,-84.731,05/03/2022,"
Malaisetrap<\/span><\/p>\n",Diptera,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG-1577-1-scaled.jpg,",38.0630000000,-84.3710000000,05/02/2022,"
Collected using an alcohol vat in a malaise trap. Near a dried creek bed that occasionally floods, mature and juvenile trees are present. Some plastic litter. Medium vegetation, strong presence of honeysuckle. Surrounded by urban\/suburban sprawl.<\/p>\n",Cicindela sexguttata,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG_9575-3-scaled.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG-1575-2-scaled.jpg,",38.0625000000,-84.7323000000,05/05/2022,"
Collected near a dry creek bed with medium vegetation, mature and young trees present. Suburban environment. Collected with a malaise trap.<\/p>\n",Insecta,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG-1581-scaled.jpg,",38.0625944000,-84.7323375000,05/02/2022,"
Collected from the area near a dry creek bed with some scattered light and heavy vegetation. Some matured trees surrounded the area. Collected using a malaise trap.<\/p>\n",Diptera,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG_9575-1-scaled.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Brummer-annina-1.jpeg,",47.458750313118074,9.019379809570326,03/29/2022,"
I found this animal near to an old tree trunk, it was slightly above the ground.<\/p>\n",Bombyliidae,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Wollschweber-Bild.png,","
Everything worked well. The extraktion was a bit difficult because I needed to figure out, where the sex organs are located. However the elektrophoresis worked well.<\/p>\n",Yes,NNTNACAGTCATCTNNTCTTGNCGTGAGNTTTNGGGNGTGCCGGGNCNNGNGAGACNGNNNCNTNNNTGCGNTCAANNANGNNNNNNGANNNNGANGCTGCNTNNAAGNNTTAANTTATATNAGNNAGNNAGNTNGGGANNNNTGNTAAGNGNTNTNTTNTNNGGGNGNTNTNGAGNNGANGNNGNAGNNGAGGGNTNNTACNANGCAAGAAAAANNAAAAAANGAANAGAAAGAANGGGGGCGCNANGGAAAGATNAGANANAGAANGAANTNNAAAAGNNGNNGGGAGGGNNNNNNNTNTNCTCTGGNNNNGGACNCNCTGCCCGGCTCGCCATGGGAATTGCTTCNNATTCANNNNANNACNCAGGAANTAACGGTGACNNAGGGNCANNANAAGANNCNNNAACCGTNAAANGACNNGAGGCTGGCGTTTATCCNTAGGCTCNGCCCCCCAGACANNCNTCACGANAANCNAAACTCAACTCANAAGNGNNGAANCCCNNCANNACTATAANNANANAATNCGNTACCNCCTGNNAACTCCCTCNNACGCNCTCCGGNNCAAACCCTGCCNCNTACCCGTATACCTGTCCNCTTACTCCCNTCATGANANTGGCNATTCCCCTNCCTCCGCAGCAGATTGCCACTNNCGNATANGNNTTCCNCNCNANNANNGCTGNGNNANCNNNNNCCCCCNCNCANNCNNNNNNCNNNGCCNNATNNNGCNNCNNNCCNTCCCNNNNN,https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/AS1-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/wipfel-stachelwanze.jpg,",47.45884196757104,9.018304282484374,04/05/2022,"
found under leaves in a forest<\/p>\n",Acanthosoma haemorrhoidale,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/ef2.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/landassel.jpg,",47.4583777113,9.0196775736,04/05/2022,"
found in dead wood<\/p>\n",Oniscus asellus,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/ef1.jpg,","",Yes,TNANACANNACTCTNNNCGANANANTTGNGGGAGTCNCGCGCNNNCTCGTCCCTCTTNNNNNGATACCATCTNGAGATGTTGTGGTCTTTNTNTNACTGCCAGTGATAAACTGNATTANGGGGGAGGATGATNTNNTNCATGATTGTCCTTATTAANTGNGCTANCGNGTGCTACGATGAAAAGCTNCAATGGGCTGCAGAAGCGCGANGCTAAGAAAATCCCNTANTAGCCANCTCANGTCGGNNNAGACTCTGCGGGTCAAAAACANNANNTTTGANTNNCNANNNNNCNNGAANCANCATACCANGNTNAG,https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/EF1-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/gemeiner-steinlaufer.jpg,",47.4583777110,9.0196775735,04/05/2022,"
found in dead wood<\/p>\n",Lithobius forficatus,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/sc2.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/keller-finsterspinne.jpg,",47.4583777110,9.0196775735,04/05/2022,"
found in dead wood<\/p>\n",Amaurobius ferox,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/sc1.jpg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/DB1-1.png,",47.4598890000,9.0182480000,04/05/2022,"
This centipede was found unter the bark of a tree stump.<\/p>\n",Lithobius dentatus,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/DB2.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/DB2-1.png,",47.4598890000,9.0182480000,04/05/2022,"
This centipede was found unter the bark of a tree stump.<\/p>\n",Tachypodoiulus niger,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/DB1.png,","
This centipede was found unter the bark of a tree stump.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/JP2-1.png,",47.4598890000,9.0182480000,04/05/2022,"
This insect was found under the bark of a tree stump. The environment was lightly damp.<\/p>\n",Chelidurella acanthopygia,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/JP2.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/JP1.png,",47.4598890000,9.0182480000,04/05/2022,"
This insect was found under the bark of a broadleaf tree stump in a lightly damp environment.<\/p>\n",Typhaea stercorea,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Bild2.jpg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/20220405_151016.jpg,",47.4566997000,9.0199946000,04/05/2022,"
Bark of a rotten tree<\/span><\/pre>\n",Oedemera virescens,Instagene Matrix,Abdomen,MiniPCR,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG-20220517-WA0013.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/20220405_151532-scaled.jpg,",47.4566997000,9.0199946000,04/05/2022,"Bark of a rotten tree<\/span><\/pre>\n",Cylindroiulus caeruleocinctus,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG-20220517-WA0013-1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Procellio-scaber.jpeg,",47.4569344619,9.0197602975,04/05/2022,"Found it in the bark of a tree<\/p>\n",Porcellio scaber,Instagene Matrix,Partial abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Wolbachia_Gel-1.jpg,","
no band<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Pardosa-lugubris.jpeg,",47.4569028265,9.0198229208,04/05/2022,"
Found it on leaves<\/p>\n",Pardosa lugubris,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Wolbachia_Gel.jpg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/oniscus-sp.-BS1.jpeg,",47.4606880000,9.0176640000,04/05/2022,"
in rotten tree trunk<\/p>\n",Oniscus,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/coelotes-terrestris.png,",47.4606880000,9.0176640000,04/05/2022,"
The spider was found under a tree bark.<\/p>\n",Coelotes terrestris,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Gel-1.jpg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Amaurobius-fenestralis-1.png,",47.4606880000,9.0176640000,04/05/2022,"
The spider was found under a tree bark.<\/p>\n",Amaurobius fenestralis,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Gel.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/rhagium-inquisitor.jpg,",47.4606880000,9.0176640000,04/05/2022,"
The insect was found under a tree bark.<\/p>\n",Rhagium inquisitor,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Gel-4.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Schrotbock-BS2-1.jpeg,",47.4606880000,9.0176640000,04/05/2022,"
under bark of felled needle tree<\/p>\n",Rhagium inquisitor,Instagene Matrix,Partial abdomen,Standard electrophoresis system,TAE,Fast Blast,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Kellerassel.jpeg,",47.4606880000,9.0176640000,04/05/2022,"
on forest floor beneath trunk<\/p>\n",Porcellio scaber,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG_20220405_091950-scaled.jpg,",47.4616173549,9.0162161487,04/19/2022,"",Strongylosoma stigmatosum,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Gel.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Paludosa-1.jpg,",47.4591684340,9.0190836469,04/05/2022,"",Cantharis,Instagene Matrix,Other,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Gel4.jpg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Curculi.jpeg,",47.4606880000,9.0176640000,04/05/2022,"
on forest floor under trunk<\/p>\n",Curculionidae,Instagene Matrix,Partial abdomen,Standard electrophoresis system,TAE,Fast Blast,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Clubiona-Saxatilis.jpg,",47.4606880000,9.0176640000,04/05/2022,"",Clubiona saxatilis,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Gel4.jpg,","",Yes,ANANANAGGGAATGGTNNNNNNTNTNNNANGNGNNNTGGGGNNNNTAACGGNNGNGNGNGGANNTNCTTNNTNNGCGANANNNANNNNTGGNNGATNTCNNCANNCCGGCTGACCNNNNNTTNNNTGNNNNNGGATGNGGCGNNNNNNGNTAGGNGNNTNTTATGNNAGTTTTGANNGGGNGGGNNGATNNTANGATNTTNNNAANNAANAAAAAAAATGGNGTAAAGAAAGNGGGGNGGGGTNTNGNCTAAGAGAANGNNTTNNNTNGAAAGGGGGGGGGGGNANNNTACCNNNNCCGNCNNCNNTNNCCCNCTCCCCCCGCCANGGNCCNNCCCCNTCCNTCAAACCAAACNCTTGCGGNNCCNNNAAACCCGGGGCCCNCTTNNTTNNCATCNTCNNNTCCTANTTAACNTACCTCANNACTNNTCCTNCCTCNTNCCCNCTCTCAAACNACCCACCTTCCCCCNCNNCCCCTCCNCCCCGCCCCCCNCCCCTTNCCNANNNACCNCTTTNTNACCAGAATNCCCCACACANNNCCCCCCCCNCNCCCCCCCACTCCTCACNNCTCCCCCCTNCCNNTCCTCNCNCANCNCNNNNNNCNNCTCNCANNNNCCNNCNGGCCGTG,https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/AZ2-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Dioprta.jpg,",47.4607289910,9.0169592605,04/19/2022,"",Drosophila,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Geophilus-Carpophagus-2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Geophilus-Carpophagus.jpg,",47.4591684340,9.0190836469,04/05/2022,"",Geophilus carpophagus,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Gel4.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Bibio-Marci.jpg,",47.4610855981,9.0133360766,04/19/2022,"",Bibio marci,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Bibio-Marci.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG_20220405_092131-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG_20220405_101617-scaled.jpg,",47.459168434,9.0190836469,05/17/2022,"",Tachypodoiulus niger,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Gel-1.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Bild4.jpg,",47.45835732250798,9.019313354492207,05/17/2022,"",Lithobius forficatus,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Bild3.jpg,",47.4599250000,9.0200000000,05/17/2022,"",Nebria brevicollis,Instagene Matrix,,Standard electrophoresis system,TAE,Fast Blast,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Walckanneria-alcuminata.jpeg,",47.4606880000,9.0176640000,04/19/2022,"
It was found under a fallen tree branch<\/p>\n",Walckenaeria acuminata,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Bild1.jpg,",47.45928583191082,9.018626708984394,05/17/2022,"",Tachypodoiulus niger,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Aculeata.jpeg,",47.4591684340,9.0190836469,04/05/2022,"
Found near by an ant hill, underneath leaves.<\/p>\n",Myrmica rubra,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Lithobimorpha.jpeg,",47.4606880000,9.0176640000,04/05/2022,"
Found underneath a fallen tree.<\/p>\n",Lithobiomorpha,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Porcellio-scaber.jpeg,",47.4606880000,9.0176640000,04/19/2022,"
It was found under a fallen tree branch<\/p>\n",Porcellio scaber,Instagene Matrix,Partial abdomen,Standard electrophoresis system,TAE,Fast Blast,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG_7681-scaled.jpg,",38.063,-84.731,05/02/2022,"
small bugs found near a small body of water and green area by a local high school.<\/p>\n",Insecta,,,,,,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG-20220510-WA0001.jpg,",47.4599000235,9.0183248519,03/29/2022,"
I found it on a dandelion flower am the edge of the forest. I collected it in the afternoon, about 2 pm.<\/p>\n",Brassicogethes aeneus,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Bild_2022-05-10_152517730.png,","",Yes,CNCCCCCNNCGAGNGNNNNCCAACTNNCNCNCNCTANGNGCTTTANGGGNNACCCCCGGCCGNAGAGCGANCCNTNTNNANTTAAANGTTCACGNAATGCAANGCACNTNNACNNTCTCCNCCNNTCGCTCTTNANNATNNANGNGNGNACNAGCTTGCGTTGNNGNNNANANCGGNNTNTCAGNNGNTTGNTCCGCNNNAGNCCNNGNCANNAACNNTTCAACCNACAACAAAAAAAAACCNCNCACCNNAAACAAGAAGCCGAGGCGCAAAGGANGCATNCANNCAAAGTCATCAAATCGAAAGCGGGGGGGGGCNGCCNTCCNCCCC,https://wolbachiaprojectdb.org/wp-content/uploads/2022/06/PY1-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/MicrosoftTeams-image-1.jpg,",47.4620000000,9.0132000000,03/29/2022,"
i found the arthropod under the bark<\/p>\n",Porcellio scaber,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/MicrosoftTeams-image.png,","
Probably it didn’t work because of the animal. It is difficult to locate the sex organs. Probably something went wrong during the pipette, because there is no band visible at all.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/WhatsApp-Image-2022-05-10-at-13.45.21.jpeg,",47.4599250000,9.0182600000,03/29/2022,"
i found the arthropod under the bark of a tree<\/p>\n",Coleoptera,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/gel-kafer.jpg,","
I made sure to crush the arthropod really well. There was only one band, there could have been a problem with my DNA extraction.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Ameise-Ilaria.jpeg,",47.4600000000,9.0100000000,03/29/2022,"
I found the ant under the bark of a tree. I lifted it with a pincette.<\/p>\n",Lasius,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Gel-Ameise-1.png,","
i made sure to crush the arthropod really well, but include the exoskeleton. the part from the arthropod that i mortared was from head to abdomen.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/kafer.jpg,",47.4599766489595,9.018289965119521,03/29/2022,"",Ptinidae,Instagene Matrix,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/ameise-1.jpg,",47.4624842840,9.0301082966,03/29/2022,"",Lasius,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/kafer-2.9.jpg,",47.459168434,9.0190836469,03/29/2022,"",Ptilinus pectinicornis,Instagene Matrix,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Kurzflugler.jpg,",47.459168434,9.0190836469,03/29/2022,"",Coleoptera,Instagene Matrix,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/5D543B8B-0740-4EA0-95FB-E7A198B8F44E-scaled.jpeg,",37.4128281,-76.5025742,04/10/2022,"
- \n
- Very small<\/li>\n
- Shelled<\/li>\n
- Antennae<\/li>\n
- Crustacean<\/li>\n<\/ul>\n",Amphipoda,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/EBDC9CCB-6283-4AA3-995E-3E7745397D2A.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/6BFEE91A-8D62-462D-B26B-5A06E907F050.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/4CA8BF76-40E9-4081-BAB7-CF1486DC204F.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/9355C1E0-7BCA-4CD1-92EE-551B3C58BF8A.jpeg,","
It was difficult to put the mixture into the gel. I poked it a bit which worried me that the DNA would come out. Flipping the holder upside down for a long time gave me a similar worry. The pellet was light brown and all gathered at the bottom.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/FC5C6692-0A08-41E9-B84D-3A608030041F-scaled.jpeg,",37.1120848,-76.5372625,04/12/2022,"
Terrestrial bug, has long legs, rough body, is in the ground<\/p>\n",Hemiptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/57105326-9636-4E70-A63B-E7DD3DC703E9.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/5474CE8B-819F-4293-9C42-3FCC2905543D.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/EBD079E3-5BE7-4517-8D95-6AE22424858B.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/52459E46-9206-4F72-8F21-21181A9D41BE.jpeg,","",No,,,, "",37.1055,76.4809,04/05/2022,"
I noticed many observations of my arthropod during this lab. Such as its two wings that it used to fly around. How it had dark hair all over it body that matched is dark color. It big greenish eye that looked bigger then its body, those eyes could see 360 degrees. I also noticed how it was pretty harmless like many other bugs it had no claws or pincher or venom.<\/p>\n",Diptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/09F0525A-6FE2-4206-A552-D20E668CC538.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/0C684F23-E805-4C32-AC16-B90CE7371950.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/34964BD5-936F-4B61-8D0F-9AB5FDBA7FE6.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/AD422756-8FF8-462D-965D-04C57D66891E.jpeg,","
Our first step was to collect the arthropod put it in a tube then killed it in rubbing alcohol. Then we picked up the dead bug with tweezer put in a Petri dish. Now that it was in the Petri dish I put under a microscope to examine it. Then transported back then we crushed up the bug then put it in a machine made it liquid, we did this a few more time then tested for wolbachia.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/2C3768EB-D9BF-4B81-8177-0B825A3E835C-scaled.jpeg,",37.105057678,-76.4814890317,04/05/2022,"
6 Legs, metallic shell.<\/p>\n",Isopoda,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/69AD7E98-2431-40AF-AB49-CD41F1B74553.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/E1750C6A-1108-4C9E-B614-9EDA1BF1ADDF-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/E6581A8E-6833-4481-8959-16A3E050E5F0-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/076F855E-3392-45DD-AEAA-74AF04B58114.jpeg,","
The DNA pellet was tiny.<\/p>\n",No,,,, "",36.1444896000,-86.8011462000,06/10/2022,"
Ladybug was red<\/p>\n",Coleoptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/57D5AEA2-2DD5-45AD-B687-A0BEC662B477.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/4E29C446-BE78-46B4-B8FA-F27E09B280D5.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/7DBFFE50-557C-495A-8E0F-7123C3BD0F93.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/BC6F102E-0629-4415-9220-C1AD759FB812.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/00CA692E-DDA5-4DC7-AC98-CB0ADD79A8C0-scaled.jpeg,",37.2334551229,-76.6704741749,04/13/2022,"
On pavement, small black ant<\/p>\n",Tetramorium immigrans,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/03937814-D46C-46B7-8D4A-DE16A1A7911D.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/6CE7897B-C779-48B6-9B4D-5CAC1B404C61.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/8FEB12B1-8F64-44C7-B63E-FCBF6DACF692.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/9A06E2C5-3612-4BA5-8E59-064127FF3BC1.jpeg,","
DNA pellet was a light purple color, no difficulties in the lab.<\/p>\n",No,,,, "",37.15,-76.482,04/05/2022,"
- \n
- It was brown<\/li>\n
- It was not super big but it was bigger than like an ant<\/li>\n<\/ul>\n",Dermaptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/24F1FA79-2E1A-48B1-84D6-DFC60C9E3447.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/EC81659C-555E-4336-9A68-733AEEE4F044.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/CE03D8FA-9E52-40C3-91C9-34EB4571937E.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/CEB91A19-CD36-4B2D-ADE1-FE7E549A5536.jpeg,","
- \n
- Something may have went wrong during DNA extraction<\/li>\n<\/ul>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/2E05DC0E-595B-49BD-9D3E-217220B5AAEF-scaled.jpeg,",37.10546196574304,-76.48133139791112,04/05/2022,"
- \n
- 6-9mm<\/li>\n
- orange, black, red<\/li>\n
- winged<\/li>\n
- pollinator<\/li>\n<\/ul>\n",Coleoptera,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/31C499F7-17A9-45C9-8EB0-75FDA50D0773.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/F7E87E16-70FC-48B5-A18E-B10BE2A72C46.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/4D812E8F-8B63-4663-8504-2895C8C85054.jpeg,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/96175137-4CDF-4F64-8E9B-6843E756FD54-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/35A5F930-1ACC-4B95-AF04-CA16A8B8B4F3-scaled.jpeg,",37.0870821000,-76.4730122000,04/13/2022,"
Oval shaped, with seven sets of legs and a hard outer shell, these creatures are best known for their ability to roll themselves into a perfectly shaped ball when threatened.<\/p>\n",Armadillidae,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/40D2319B-37D6-46E7-95F7-5F315FD0392A.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/9BF41D9A-4C7A-4C9A-8122-EDB21F793497.jpeg,","
The control was not clear so we are unsure about the results. Color of pill was brown.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/CB93F3D7-57C7-4ACF-950E-2F608D420490-scaled.jpeg,",37.1120848000,-76.5372625000,04/05/2022,"
- \n
- Brown and grey<\/li>\n
- Slow<\/li>\n
- Beneath a rock<\/li>\n
- About a cm long<\/li>\n<\/ul>\n",Isopoda,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"","
Control lane 5 was positive for arthropod when it should not have.<\/p>\n",No,,,, "",,,,"",,,,,,,"","",,,,, "",-84.8220000000,39.8220000000,03/22/2022,"",Geophilomorpha,,Abdomen,Standard electrophoresis system,,,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/57E4F2A1-B7A1-41EB-B538-E98270D20CE2.jpeg,",37.2424206000,-76.6643728000,04/05/2022,"
It\u2019s a mosquito. It\u2019s not very large, and one of its wings in wrinkled. It has long, orange legs, large abdomen, rounded\/small head, and it has antennas coming out of it\u2019s head.<\/p>\n",Diptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/EA907391-C9A8-43FE-98DE-623A1F61B7E2.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/BDBB910D-2326-420E-A846-3E212F6CCCAF.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/134248A9-2F59-433F-908A-7D3DE5D42022.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/8B4BD6DC-5AFC-492D-A8C7-90DE09DD7360.jpeg,","
Our controls didn\u2019t turn out the way they should, so we\u2019re not completely confident in our results. However, my result should be at least medium confident because both of mine were positive.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/3159E882-EF16-43B8-B19E-37C626DD605F-scaled.jpeg,",37.0672839,-76.514138,04/07/2022,"
The Arthropod was found under a rock, and it was moving slowly. It tried to run when the rock was removed. The bug was six legged with a long body. It had large beaded eyesIt also had long antennae like structures and pincer like structures on the back side. It had a ribbed back, no wings, and three body segments. The coloration was black, brown, and generally dark.<\/p>\n",Dermaptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/08356EF2-C305-489B-BB62-8C8DCD1F20A4.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/DF30AA47-8845-4CA0-89AB-841A61B599EA.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/3BFF9460-58AF-439B-A16C-22ABF6C6EF64.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/F0B535BF-2C6F-4F3A-AF64-A339CA685D55.jpeg,","
There were not any mistakes made in the lab that were known of. The only difficulties came from managing the materials properly, but a system was worked out that was effective.<\/p>\n",Yes,,,, "",37.5407246000,-77.4360481000,03/31/2022,"",,Thermoscientific GeneJet Genomic Purification,Partial abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/20220415_160132-scaled.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/F5F6FE91-00D7-4EFF-BBAC-22B639CE4647.jpeg,",39.8197200000,-84.8994266200,03/30/2022,"
Was curled up when caught<\/p>\n",Diplopoda,Thermoscientific GeneJet Genomic Purification,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/08764AE9-0A0F-4AEA-8D31-A8B2F6FEBF90-scaled.jpeg,","
We did everything correctly<\/p>\n",Unknown,,,, "",37.1051358618,-76.4809681018,04/14/2022,"
-black<\/p>\n
-visually matches a beetle<\/p>\n
-hard exoskeleton<\/p>\n
-wings<\/p>\n",Coleoptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/29A639C9-BAF1-4104-9412-B9679F92FC7A.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/EEF32948-8E81-463F-8FF0-48D1AE4A0E65.jpeg,","
-Dissecting abdomen was a bit confusing<\/p>\n
-Injecting dna into gel was a little difficult<\/p>\n
-Dna was brown and stuck to the bottom of tlhe vial<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/2E8FB3F1-6819-4815-9192-760F8EAAE991.jpeg,",37.2230420000,-76.6512370000,04/11/2022,"
The arthropod was found in a terrestrial environment near some small rocks. It was a wooded area near shrubs and flowers. Flying ants have elbowed antennas and long fore wings. It has a thin waist constricted at the thorax and small hind wings. The darkly-colored ant is slightly larger than a grain of rice.<\/p>\n",Hymenoptera,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/77ED61B7-8E56-4EA4-A37D-912941F80678.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/8F61F4ED-9F51-42C2-A868-72C8430176F4.jpeg,","
Possible result mistakes:
\ninsufficient amount of DNA, DNA extraction failure<\/p>\nPellet Description: small, visible black segments of body churned up<\/p>\n
<\/p>\n
<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/Wallbakia-scaled.jpg,",37.1175667793,-76.4075283792,04/11/2022,"
It is a small creature that is brown, has small legs, wings are large (located on its back), and has 2 black eyes. Also, it has a pair of wings, 3 pairs of legs, large head, feet look like they are spiky-ish, body is small, and wings are bigger than the head.<\/p>\n",Hemiptera,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/Control-Arthropod.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/Control-Walbachia.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/Me-Wallbachia.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/Me-Arthropod.jpeg,","
We had either contamination in our gels or incorrectly placed controls.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/8066010C-D2B9-42A0-8638-CF4602E4D663-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/D56CA34F-A98B-40AF-8CAF-87594CEA258B-scaled.jpeg,",89.99999743879611,9.916176443465972,04/12/2022,"
2 wings<\/p>\n
4 legs<\/p>\n
Shiny black<\/p>\n
brown beedy eyes<\/p>\n
hair on the body<\/p>\n
sliver glare on the skin<\/p>\n
proboscis<\/p>\n
was dead when found.<\/p>\n
<\/p>\n",Diptera,Edwards Buffer,,,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/8F4BD1A5-DE1B-40FE-A54B-7ECC3896CE59.jpeg,","
Both gels did not show. I think because it was all ready dead when founded.<\/p><\/blockquote>\n",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/1BC7F2BE-AC03-42F4-AF9A-8DA0D7B920AD-scaled.jpeg,",37.2301182581,-76.6549002502,04/11/2022,"
– moves quicker than ants around them, found in damp soil.<\/p>\n",Hymenoptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/AACF0C14-1345-41E0-83C1-4C5D6CA39392.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/71DCA077-9813-4273-A389-1F8DFBFAA247.jpeg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/EF8E325A-F9CE-4BB5-9FCB-4CA24707DDE2.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/B011CE94-4ED6-4520-8281-3AE5A86F6B2B.jpeg,",37.1438246000,-76.4093851000,04/07/2022,"
Red-ish-brown, six legs, an inch long, antennae an inch and a half, two sets of wings, external shell, hair plates<\/p>\n",Blattodea,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/76B3C1BE-0D02-4293-971B-E1C0C4A26151.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/180B4BCC-B802-43FA-9E80-FE964F9CBB27.jpeg,","
Either there was contamination in the gel, or the DNA was placed incorrectly into the gel.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/CEBEC44F-AF33-4DFB-8D04-D594FB0C24A3-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/472D1898-D0C7-4FAA-80CC-F413AA3D1CE8-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/552894A2-E677-465D-882B-C4E6BE11C4CF-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/5DD5C488-83E9-477E-A635-ACB2AC2589DB-scaled.jpeg,",37.2824820883,-76.6542145293,04/10/2022,"
big (size of thumb nail) , brown, and has 6 legs, had two sets of wings, has a hard shell<\/p>\n",Hexapoda,DNeasy (Qiagen),Abdomen,MiniOne,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/C84E79EF-D8D8-47B1-9F86-FC812326A184-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/2839F3FE-BAF5-4666-8D61-D8CF6B4EF9F1-scaled.jpeg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/E4283C1E-6556-43AA-AB67-9735B6ED001B-scaled.jpeg,",37.2826523720,-76.6541411814,04/10/2022,"
- \n
- Black body<\/li>\n
- Red legs (6)<\/li>\n
- Antennae<\/li>\n
- 1 set of wings<\/li>\n
- Size: pinky nail<\/li>\n<\/ul>\n",Hexapoda,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/8705D872-5D02-4164-8F6E-5AC344BBFA31.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/F591A6DE-6A0A-4174-B422-0EC061D36B58.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/97D9EB76-EE60-462E-83BA-7CA66ABFBFFC.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/A9A27B02-EFEA-4B02-ACE4-BF131517E29B.jpeg,","
The DNA pellet at the bottom looked like a tan gunk glob.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/D7B9AE25-189C-4E42-93A9-A2CB3B7C3CF1-scaled.jpeg,",37.1078128946,-76.4377999627,04/10/2022,"
Was not moving, 4 yellow legs, large wings, visible stinger, about an inch in length, black<\/p>\n
<\/p>\n",Hymenoptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/C72222DE-59D0-450E-9BF4-9EB1A2C37A16.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/11CC51C6-58EF-4B96-9940-C8508AB28C5D.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/6FF3C769-28CD-4998-8FD4-42215307790F.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/F3135ADD-1354-4234-A322-1724CB1A7E15.jpeg,","
During DNA Extraction, there may have been bubbles when adding the Cell Lysis Buffer. During Gel Electrophoresis, the gel may have been poked through when adding the PCR product. The DNA pellet had a dissolved solution, and it blended to become the same color.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/95176695-5159-4B24-A16C-5E002C09B085-scaled.jpeg,",37.0820000000,-76.2420000000,04/09/2022,"
Long, skinny, 118 legs (59 on each side), two antennae, golden-brown\/orange\/yellow color, moves pretty fast, segmented body (2 legs per segment), back legs are elongated, a little shorter in length than pinky finger.<\/p>\n",Geophilomorpha,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/583E8D32-F326-401F-8894-D4A2458C935C.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/99178002-5D8B-4265-B530-3B87DAACCBEE.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/A703AEBB-1CFF-44F1-9533-F95E94F8A91C.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/0D96FE02-2568-49D6-9CBB-86DEA1E11185.jpeg,","
DNA may not have been extracted correctly, or arthropod may not have been crushed completely.<\/p>\n
The DNA pellet was a tan-ish color at the bottom of the tube.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/4FCB3A59-F3BC-48D3-87EC-391088E7949A.jpeg,",37.1120848000,-76.5372625000,04/12/2022,"
It looks health, it is black.<\/p>\n",Chilopoda,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/D-Block-Wolbachia-2-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/D-Block-Arthropod-2-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/D-Block-Wolbachia-1-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/D-Block-Arthropod-1-1.jpeg,","
Maybe I accidentally mix with bubble in the process.<\/p>\n",No,,,, "",37.1120848,-76.5372625,04/10/2021,"
6 legs, 2 head antennas, exoskeleton, 3 butt antennas
\nSpots and strips with a deep brown and beige color<\/p>\n",Orthoptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/242D7CE5-CE27-4084-B4C0-32BFAA797C59.jpeg,","",Yes,,,, "",37,42146,76,52820,04/10/2022,"The arthropod was hiding under a rock from the sun, searching for a cooler environment. It rolled up into a ball for protection when it was disturbed for the observation. It had seven pairs of legs. It had simple eyes, uropods, a pair of prominent antennae. It had an exoskeleton with palates.<\/p>\n",Isopoda,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/A9F790F2-2D4A-4779-B3BD-E79726BC8EA1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/0E13DCF7-7788-4019-925E-94824DC47DA2.jpeg,","
The PCR was working correctly. The concentration of the gel might have been too high. This might have lead to errors in resolving the different bands.<\/p>\n",No,,,, "",37.1714240459,-76.4344909816,04/12/2022,"
Ant head it had. Tweezer looking tail. Antennas. 6 legs<\/p>\n",Dermaptera,Edwards Buffer,Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/613F00CB-5A97-4F09-9C23-AA51B3EF83CB.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/9064A8D9-F4BE-4819-987D-262C4DFDBDB0.jpeg,","
Quite astonishing that there was no wolbachia and arthropod considering that my bug was under the class dermaptera.<\/p>\n",No,,,, "",37.1065094,-76.4801591,04/04/2022,"
When it was found the ant was walking around in circles \u00a0on the ground with a quick pace. It was brown in color, had three body sections (a large lower section and a thin mid section), six legs (3 per side), 2 antennas, and two black eyes<\/p>\n",Hymenoptera,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/99E6851C-2AD4-405C-83A8-6F53DAC29E35.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/1EA3D16A-E425-49DE-9BB2-9A9934028DD0.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/CE11F49A-C519-43A7-A554-F9F2F1756DB8.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/A6F2731C-1054-41E8-B5F1-381C60C055EA.jpeg,","
Enough DNA was extracted to have tested positive wolbachia band, but something must have gone wrong with the Arthropod PCR. This could have resulted due to the liquids being incorrect. The positive also didn\u2019t result in an arthropod band.<\/p>\n",Yes,,,, "",37.1708943,-76.4306161,04/11/2022,"
three main parts of body<\/p>\n
with other ants<\/p>\n
in warm house<\/p>\n
six legs<\/p>\n",Hymenoptera,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/D-Block-Wolbachia-2.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/D-Block-Arthropod-2.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/D-Block-Arthropod-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/D-Block-Wolbachia-1.jpeg,","
Not extracting DNA right<\/p>\n
Contamination<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/90CF8C14-CE66-43E9-BD45-8B70177E45B2.jpeg,",37.1050241235,-76.4811223606,04/12/2022,"
Around other aurthropods, moist environment, under rock,\u00a0Long, round, many legs, brown, green part at end of tail,<\/p>\n",Polydesmida,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/6D8DBEC4-676F-498E-9E3A-38334004D868.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/E349EEAC-44DC-4ECA-8776-B280AFE36C6E.jpeg,","
A lot of people didn\u2019t get results, so there may have been an error extracting the DNA.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/2A4D20A2-893F-4C04-B54A-F6528853439C-scaled.jpeg,",37.1120848000,-76.5372625000,09/15/2021,"
\u2022 2 winged
\n\u2022 2 antennae
\n\u2022 2 black-eyes (located in front right below antennae)
\n\u2022 Tan\/beige color
\n\u2022 Rolled up sucker mouth (Like a butterfly)
\n\u2022 3 sets of legs
\n\u2022 Legs have hair\/is jagged
\n\u2022 3 segments<\/p>\n",Lepidoptera,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/74069DDC-016F-4665-8EF1-96050B2ACFCA.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/7194FC39-F549-482B-A5FB-A9081634F50E.jpeg,","Potential Mistakes:<\/p>\n
- \n
- DNA extraction wasn\u2019t successful (didn\u2019t extract enough)<\/li>\n
- Didn\u2019t place DNA solution correctly inside the cell of the gel<\/li>\n
- Something wrong with PCR Master Mix<\/li>\n<\/ul>\n
Pellet Description:<\/p>\n
- \n
- Small, dark brown, rested at bottom of microcentrifuge tube<\/li>\n<\/ul>\n",No,,,,
"",37.0229000000,76.3452000000,04/12/2022,"
- \n
- my arthropod was near leaves and trees kinda close to sap I found on a tree. Because it is an ant it was found with a cluster of others. To allow for easier catching I put an apple on my deck to allow for a few to come. To my surprise \u00a0many an f I was able to collect. The ant was crawling \u00a0around taking some of the apple pieces back to the tree. Some descriptions of my ant would be it\u2019s size and color. This carpenter ant was a burgundy color and quite small in size. Compared to the other ants crawling around it the size was quite small but the burgundy stood out. It had hints of black ( the antenna and other small features).<\/li>\n<\/ul>\n",Hymenoptera,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/176A9CB0-FB6C-4499-A663-995FDF4E563C.jpeg,","
Honestly, they weren’t any mistakes or difficulties. The only one I can point out is the fact that our pipettes were breaking, and also we couldn’t complete the last part of our experiment. However, it was completed by another class. Although there were only a few hiccups throughout the way a few mistakes were made. Whether that be not understanding what to do or not paying, much attention.<\/p>\n",No,,,, "",39.1292003000,-84.4595981000,04/14/2022,"
These are cultured fruit flies purchased by Carolina Biological and arrived on March 23, 2022. The strain was miniature apterous.<\/span><\/span>\u00a0<\/span><\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/thumbnail_Slide1-9.jpg,","",No,,,, "",39.1377808000,-84.4693665000,04/14/2022,"
From Carolina Biological, Stock 3\/23<\/p>\n
Drosophila were Miniature<\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/11-20D-8.jpg,","",No,,,, "",39.1295728000,-84.4597957000,04/14/2022,"
From Carolina Biological, Stock 3\/23<\/p>\n
Drosophila were Miniature<\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/11-20D-7.jpg,","",No,,,, "",39.1292003000,-84.4595981000,04/14/2022,"
These are cultured fruit flies purchased by Carolina Biological and arrived on March 23, 2022. The strain was miniature apterous.<\/span><\/span>\u00a0<\/span><\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/thumbnail_Slide1-8.jpg,","",No,,,, "",39.1377808000,-84.4693665000,04/14/2022,"
From Carolina Biological, Stock 3\/23<\/p>\n
Drosophila were Miniature<\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/11-20D-6.jpg,","",No,,,, "",39.1292003000,-84.4595981000,04/14/2022,"
These are cultured fruit flies purchased by Carolina Biological and arrived on March 23, 2022. The strain was miniature apterous.<\/span><\/span>\u00a0<\/span><\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/thumbnail_Slide1-7.jpg,","",No,,,, "",39.1377906000,-84.4695715000,04/19/2022,"
Wild caught drosophila at restaurant.<\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/thumbnail_Slide1-4.jpg,","
Standard DNA extraction, duplex PCR and gel electrophoresis per Discover the Microbe Within protocols.<\/p>\n",No,,,, "",39.1377808000,-84.4693665000,04/14/2022,"
From Carolina Biological, Stock 3\/23<\/p>\n
Drosophila were Miniature<\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/11-20D-5.jpg,","",No,,,, "",39.1292003000,-84.4595981000,04/14/2022,"
These are cultured fruit flies purchased by Carolina Biological and arrived on March 23, 2022. The strain was miniature apterous.<\/span><\/span>\u00a0<\/span><\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/thumbnail_Slide1-6.jpg,","",No,,,, "",39.1292003000,-84.4595981000,04/14/2022,"
These are cultured fruit flies purchased by Carolina Biological and arrived on March 23, 2022. The strain was miniature apterous.<\/span><\/span>\u00a0<\/span><\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/thumbnail_Slide1-5.jpg,","",No,,,, "",39.1377808000,-84.4693665000,04/14/2022,"
From Carolina Biological, Stock 3\/23<\/p>\n
Drosophila were Miniature<\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/11-20D-4.jpg,","",No,,,, "",39.1377808000,-84.4693665000,04/14/2022,"
From Carolina Biological, Stock 3\/23<\/p>\n
Drosophila were Miniature<\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/11-20D-3.jpg,","",No,,,, "",39.1292003000,-84.4595981000,04/14/2022,"
These are cultured fruit flies purchased by Carolina Biological and arrived on March 23, 2022. The strain was miniature apterous.<\/span><\/span>\u00a0<\/span><\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/thumbnail_Slide1-4.jpg,","",No,,,, "",39.1377906000,-84.4695715000,04/19/2022,"
Wild-caught fruit fly at restaurant.<\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/Slide1-1.jpg,","
Standard protocol for DNA extraction and gel electrophoresis to screen for Wolbachia<\/p>\n",No,,,, "",39.1377808000,-84.4693665000,04/14/2022,"
From Carolina Biological, Stock 3\/23<\/p>\n
- \n
- Drosophila were Miniature<\/li>\n<\/ul>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/11-20D-2.jpg,","",No,,,,
"",39.1292003,-84.4595981,04/11/2022,"
These are cultured fruit flies purchased by Carolina Biological and arrived on March 23, 2022. The strain was miniature apterous.<\/span><\/span>\u00a0<\/span><\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/thumbnail_Slide1-3.jpg,","",No,,,, "",39.1377808000,-84.4693665000,04/14/2022,"
From Carolina Biological, Stock 3\/23<\/p>\n
-Drosophila were miniature<\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/11-20D-1.jpg,","",No,,,, "",39.1292003000,-84.4595981000,04/11/2022,"
These are cultured fruit flies purchased by Carolina Biological and arrived on March 23, 2022. The strain was miniature apterous.<\/span><\/span>\u00a0<\/span><\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/thumbnail_Slide1-2.jpg,","
<\/p>\n
<\/p>\n",No,,,, "",39.1295990000,-84.4597601000,04/14/2022,"
From Carolina Biological, Stock 3\/23<\/p>\n
Drosophila was miniature.<\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/11-20D.jpg,","",No,,,, "",39.1292003000,-84.4595981000,04/11/2022,"
This is a strain from Carolina Biological that arrived on March 23. The type was miniature apterous.<\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/thumbnail_Slide1-1.jpg,","",No,,,, "",39.1296765525,-84.4594607250,04/14/2022,"
From Carolina Biological, Stock 3\/23<\/p>\n
-Drosophila were Miniature<\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/thumbnail_Slide3.jpg,","",No,,,, "",39.1292003,-84.4595981,04/11/2022,"
These are cultured fruit flies purchased by Carolina Biological and arrived on March 23, 2022. The strain was miniature apterous.<\/p>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/thumbnail_Slide1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/0A3CBD93-975E-4B2F-9DBD-5DA4D6B712C8-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/89227DF8-A5D9-49A9-97BB-B383D8BC3C7F-scaled.jpeg,",39.8196542606,-84.8994266281,03/30/2022,"
tiny, green, it can fly<\/p>\n",Hymenoptera,Thermoscientific GeneJet Genomic Purification,Abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/08764AE9-0A0F-4AEA-8D31-A8B2F6FEBF90-scaled.jpeg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/2022-04-18T15_22_26.843Z-1-scaled.jpg,",39.8195879000,-84.8995345000,03/30/2022,"
Under a damp log(crawling around on the dirt), tried running away when collecting<\/p>\n",Isopoda,Thermoscientific GeneJet Genomic Purification,Abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/2022-04-18T15_22_04.077Z-1.jpg,","
Originally our gel did not set up properly so we had to redo it. And when loading the gels we put it backwards so when we put it in the wash buffer it washed out.<\/span><\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/where-found-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/winston-scaled.jpg,",845359.0000000000,394908.0000000000,03/30/2022,"
Walking under a log in wet soil. Curled into a small circle when poked.<\/p>\n",Armadillidae,Thermoscientific GeneJet Genomic Purification,Abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/gel-up-close-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/gel-distance-scaled.jpg,","
There was a lot of tubes passing around the table, which made it hard to determine whether or not I had used one, or even if I had only used it once or the recommended amount.<\/p>\n",No,,,, "",39.8325059000,-84.9019057000,03/31/2022,"",Scolopendromorpha,Thermoscientific GeneJet Genomic Purification,Abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"","
We switched wells 2 and 3 when filling them in<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/2022-04-18T16_00_22.560Z-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/IMG_9650-scaled.jpeg,",39.8218679112,-84.8990507706,06/07/2022,"
It was a rolly Polley and it was under a rock<\/p>\n",Armadillidium vulgare,Thermoscientific GeneJet Genomic Purification,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","
One person would make the gel and the other would do the pcr.<\/p>\n",Unknown,,,, "",39.81719,84.90076,03/30/2022,"
Small body, black and brown body, hairy legs<\/p>\n",Araneae,Thermoscientific GeneJet Genomic Purification,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",No,,,, "",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/7EF3B69E-9527-4253-9657-3FDA1139E4FA-scaled.jpeg,",39.8169300000,-84.9010800000,03/30/2022,"
The roly-polys were together under a log, where it was cold and wet. The roly-polys liked to stay away from sunlight, mostly likely in dry and dark places in the dirt. I observed that it had 7 sets of legs, a hard plated dark grey exoskeleton, an oval body, and curled into a ball when touched, so I was able to identify that it was a pillbug.<\/p>\n",Armadillidium vulgare,Thermoscientific GeneJet Genomic Purification,Abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/560FBFAF-15F6-47F8-948F-4EA56B7D803E-scaled.jpeg,","
I think that the DNA was not thoroughly cleaned and that enough was pulled out of the cells in the reproductive tissues of my arthropod.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/5A7197DA-1638-4C00-9B5A-24BE8E67B862.png,",82.89839,39.82095,03/30/2022,"
Long body and wings, dark brown\/black, 2 long antennae, six legs<\/p>\n",Lepidoptera,Thermoscientific GeneJet Genomic Purification,Abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/2B3D14CC-3D69-45C9-90FA-DF8194C19944-scaled.jpeg,","",Unknown,,,, "",39.817481,-84.900545,06/12/2022,"
In the gorge, under a log. In a wet area<\/p>\n",Coccinellidae,Thermoscientific GeneJet Genomic Purification,Abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/20220415_113252-scaled.jpg,","",Unknown,,,, "",39.82042,84.89910,03/30/2022,"
![\"\"](\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2022\/04\/D6DE3908-54D3-42C7-A3AD-843C2706DB1F-225x300.jpeg\")
<\/p>\n",Armadillidae,Edwards Buffer,Abdomen,Standard electrophoresis system,PBS,GelGreen,"","![\"\"](\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2022\/04\/AB6FB229-8C0F-4B75-8531-25D2317DAA9A-225x300.jpeg\")
<\/p>\n",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/ADB23D8B-608B-47E4-ACD7-371D3178D8C3-scaled.jpeg,",39.8289369000,-84.8902382000,03/30/2022,"Black, in a tree, very small, Roly-Poly<\/p>\n",Armadillidae,Thermoscientific GeneJet Genomic Purification,Abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/E8E14C35-90AF-4B67-AB6D-97CD28423F78-scaled.jpeg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/2022-04-20T14_36_03.872Z-scaled.jpg,",39.8200000000,-84.8990000000,03/30/2022,"
Yellow centipede, small, found under a rock with wet dirt, and a lot of legs, “scaly”, little antennas<\/p>\n",Chilopoda,Instagene Matrix,Partial abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/2022-04-20T14_35_45.639Z.jpg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/2022-04-20T14_37_43.319Z-scaled.jpg,",39.8200000000,-84.8990000000,06/12/2022,"
Black, under a rock, and near dirt.<\/p>\n",Diplopoda,Thermoscientific GeneJet Genomic Purification,Partial abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/2022-04-20T14_34_45.446Z.jpg,","
N\/A<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/black-garden-ant_1200x675.jpg,",39.8210000000,-84.8990000000,03/30/2022,"
6 legs, all black, centimetre long, shorter legs<\/p>\n",Hymenoptera,Thermoscientific GeneJet Genomic Purification,Abdomen,,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/20220415_160026-1-scaled.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/IMG_0162-1.jpg,",39.8227600000,-84.9018100000,03/31/2022,"
green, near waters little chilly, around trees and under a wooden table.<\/p>\n",Culicidae,Thermoscientific GeneJet Genomic Purification,Whole arthropod,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/IMG_0161.jpg,","
During the DNA extraction step when adding AW 1 buffer to the solution, the solution turned pink<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/unnamed-1-scaled.jpg,",39.8200000000,-84.8990000000,03/30/2022,"
It was very fast and tried to run away. It was under a log in the moist dirt.<\/p>\n",Coleoptera,Thermoscientific GeneJet Genomic Purification,Whole arthropod,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/IMG_5250-1-scaled.jpg,","
After adding AW2 buffer and centrifuging, my solution turned pink.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/IMG-5203-scaled.jpg,",39.817580,-84.901303,03/30/2022,"
Under a log in a wet environment with a\u00a0 plentiful of leaves around it.<\/p>\n",Armadillidium vulgare,Thermoscientific GeneJet Genomic Purification,Whole arthropod,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/IMG_5250-scaled.jpg,","
During DNA extraction when the AW 1 and AW 2 buffers were added the solution turned pink<\/p>\n",No,,,, "",39.8214875311,-84.8983473895,,"",,,Whole arthropod,MiniOne,Other,SYBR Safe,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/F86A6052-4198-48F4-B548-5FF1CD013055-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/01F9A998-310B-4F2B-8473-F4F126E68560-scaled.jpeg,",39.8227610000,-84.9017500000,03/02/2022,"
- \n
- Beetle<\/li>\n
- 1\/4 inch<\/li>\n
- Shiny black<\/li>\n
- 4 yellow-brown spots<\/li>\n
- 6 legs<\/li>\n
- Found beneath wooden crate near rocks and several trees<\/li>\n<\/ul>\n",,Thermoscientific GeneJet Genomic Purification,Whole arthropod,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/E8288F5E-2F93-478D-B2CA-8B9DFA6CAC3C.jpeg,","
The addition of Buffer AW1 during DNA Purification cases the solution to turn pink.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/20220323_092124-1-scaled.jpg,",43.3951833384,-72.3341750159,03/23/2022,"",Coccinellidae,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,GelRed,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/20220323_092124-scaled.jpg,",432100.1,721314.8,02/23/2022,"
Faint color on the outer shell<\/p>\n",Hexapoda,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,GelRed,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/20220323_092112-scaled.jpg,",432100.1,721314.8,03/26/2022,"",Entognatha,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,GelRed,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/image1-2-rotated.jpeg,",43.521298356439765,-72.1469063105831,03/23/2022,"
dead upon collection<\/p>\n",Gryllidae,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,GelRed,"","",Unknown,,,, "",43.5328000000,-72.1266000000,03/07/2022,"
Was found on a clay pot outside of building, multiple mosquitos were in the area.<\/p>\n",Diptera,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,GelRed,"","
Followed standard protocol.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/20220323_092112-1-scaled.jpg,",43.4021261328,-72.3336793602,03/23/2022,"",Calliphoridae,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,GelRed,"","",No,,,, "",43.5328000000,-72.1266000000,03/07/2022,"
Arthropod was inside of enclosed environment, was not outside in the elements.<\/p>\n",Musca,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,GelRed,"","",Unknown,,,, "",39.1708672000,-84.3677696000,05/21/2021,"
Female decim<\/p>\n",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,,MiniPCR,TBE,GelGreen,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_female_abdomen_1-scaled.jpg,",39.1653593000,-84.3713935000,05/22/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel7a.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_male_abdomen-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_side-scaled.jpg,",39.1653593000,-84.3713935000,05/20/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel6a-1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_side-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_male_abdomen-scaled.jpg,",39.1653593000,-84.3713935000,05/20/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel1a.jpg,","",No,,,, "",39.1297540000,-84.4614760000,05/21/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel-623-13.jpg,","",No,,,, "",39.1297540000,-84.4614760000,05/21/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel-623-14.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_male_abdomen-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_side-scaled.jpg,",39.1653593000,-84.3713935000,05/20/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel8a.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel6a-1.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_male_abdomen-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_side-scaled.jpg,",39.1653593000,-84.3713935000,05/20/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel5a.jpg,","",No,,,, "",39.8236539000,-84.9001014000,04/01/2022,"
A tiny mite that looks like a crab<\/p>\n",Arachnida,DNeasy (Qiagen),Other,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/IMG_1484-scaled.jpeg,",39.8261523000,-84.8928312000,03/30/2022,"
Black beetle, small with lines on the back.<\/p>\n",Pterostichus,Thermoscientific GeneJet Genomic Purification,Abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/hawley.jpg,","
My DNA for my bug was the only one that showed on the gel. My bug was negative for Wolbachia.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/1649148006.936077-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/1649148017.414411-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/1649148025.759644-scaled.jpg,",39.8207312184,-84.8991612613,03/30/2022,"
Small, round, segmented brown isopoda bug. Found under a wet log, where it was in the dirt.<\/p>\n",Armadillidae,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/D95BAFD4-9EEF-4681-933B-FFA435D71369-scaled.jpeg,",39.8282551000,-84.8984775000,03/30/2022,"
Emerald green, iridescent, brown legs, vertical imprinted pattern on shell, elongated thorax<\/p>\n",Chlaenius,Thermoscientific GeneJet Genomic Purification,Partial abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/20220415_113252-1-1.jpg,",39.8227524000,-84.9017282000,,"
The black fly had 8 legs, big eyes, and a noticeable head, thorax, abdomen, with wings.<\/p>\n",Hermetia illucens,Thermoscientific GeneJet Genomic Purification,Abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/20220415_113252-1-1-1.jpg,","
It was very straight forward and very simple to do but Wolbachia is not present<\/p>\n",No,,,, "",39.8174090000,-84.0000000000,03/31/2022,"
Moves quickly<\/p>\n
Rolls easily<\/p>\n
1cm long<\/p>\n
0.3cm wide<\/p>\n
Rolly Polly!<\/p>\n",Isopoda,Thermoscientific GeneJet Genomic Purification,Partial abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/2022-04-18T15_24_04.335Z-scaled.jpg,","
We made an error in pipetting into well 4.<\/p>\n",Unknown,,,, "",39.8229140000,-84.8995520000,03/30/2022,"
- \n
- grey color<\/li>\n
- roll into ball<\/li>\n
- hard shell<\/li>\n
- multiple legs<\/li>\n
- under wet log<\/li>\n<\/ul>\n",Isopoda,Thermoscientific GeneJet Genomic Purification,Abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/2022-04-18T15_25_56.671Z-scaled.jpg,","",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/10/2BD08C07-B14F-48B2-A998-0057D2602625-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/10/E23BED97-024B-49E4-AF5D-1441979E2E76-scaled.jpeg,",42.1164391255,-76.2724974980,09/10/2021,"",Hymenoptera,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/11/FF9689C1-D4E5-4B30-B8BC-C192B68232AF-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/11/17AE933B-CF84-4298-A182-530643D10B14-scaled.jpeg,","
Wolbachia gel electrophoresis image (left)-<\/p>\n
Well 1: DNA ladder<\/p>\n
Well 3: Arthropod DNA<\/p>\n
Well 4: Positive Wolbachia control<\/p>\n
Well 5: Negative Wolbachia control<\/p>\n
<\/p>\n
Arthropod gel electrophoresis image (right):<\/p>\n
Well 1: DNA ladder<\/p>\n
Well 3: Arthropod DNA<\/p>\n
Well 4: Positive arthropod control (note: a portion of the positive arthropod control was released outside of the well)<\/p>\n
Well 6: Negative arthropod control<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/unnamed-scaled.jpg,",42.4884618000,-71.1329685000,09/14/2021,"
The insect is white with one black spot on each wing. About an inch to an inch and a half total wingspan.<\/p>\n",Pieris rapae,Edwards Buffer,Partial abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/Untitled.jpg,","
Duplex PCR reaction was used.<\/p>\n",Unknown,Provided by Wolbachia Project,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/Capture2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/Capture3.jpg,",42.4952872000,-71.1490872000,09/14/2022,"
The pill bug was caught by someone else, but I had the chance to use the pill bug for the Wolbachia experiment. The pill bug caught is an isopod that is grey and very tiny.<\/p>\n",Armadillidae,Edwards Buffer,Partial abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/111.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/Capture100.jpg,","
DNA Extraction protocol<\/a><\/p>\n",Yes,Primers provided by Wolbachia Project,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/Wolbachia-Insect-Collection-Sep-14-2021-at-3_24-PM.png,",42.4792618000,-71.1522765000,09/14/2021,"
Very tiny<\/p>\n",Diptera,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/Screenshot-2022-03-30-2.32.31-PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/Screenshot-2022-03-30-2.34.08-PM.png,","
Duplex PCR was performed.<\/p>\n
Edvotek was used for PCR<\/p>\n",Unknown,Primers used were from Wolbachia Project,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/Wolbachia-Insect-Collection-Sep-15-2021-at-12_57-PM-1.png,",42.4833831019,-71.1421565666,09/14/2021,"",Hymenoptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/Screenshot-2022-03-30-1.54.08-PM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/03CACF9B-8495-47A7-B73C-0487107CAE6A-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/9D011B6D-8516-43FB-AD7E-C42621290DFF-scaled.jpeg,",42.4952872,-71.1490872,09/14/2021,"
Leaf hopper found in Woburn Memorial highschool grass. It is green and small.<\/p>\n",Hemiptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/9D011B6D-8516-43FB-AD7E-C42621290DFF-1-scaled.jpeg,","
Duplex PCR reaction was preformed.<\/p>\n",Unknown,Primers provided by Wolbachia Project,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/image_6487327.jpg,",42.4788017078,-71.1494479922,09/14/2021,"
this is a honey bee I caught outside.<\/p>\n",Apis,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/image_6487327-1.jpg,","
DNA Extraction protocol<\/a><\/p>\n",No,provided by wolbachia project,,, "",42.4830633000,-71.1435832000,09/27/2019,"
Wolbachia positive fruit fly from The Wolbachia Project<\/p>\n",Diptera,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/phonto-6-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/Screenshot-2022-03-30-1.28.01-PM.png,","
Duplex PCR was performed<\/p>\n",Yes,Primers provided by Wolbachia Project,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/Biotech-Wolbachia-Insect-.png,",42.4830633000,-71.1435832000,09/14/2021,"
This is a picture of an ant.<\/p>\n",Insecta,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/Gel-Wolbachia.jpg,","
- Drosophila were Miniature<\/li>\n<\/ul>\n",Drosophila melanogaster,Thermoscientific GeneJet Genomic Purification,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/11-20D-2.jpg,","",No,,,,
"",39.1292003,-84.4595981,04/11/2022,"
- my arthropod was near leaves and trees kinda close to sap I found on a tree. Because it is an ant it was found with a cluster of others. To allow for easier catching I put an apple on my deck to allow for a few to come. To my surprise \u00a0many an f I was able to collect. The ant was crawling \u00a0around taking some of the apple pieces back to the tree. Some descriptions of my ant would be it\u2019s size and color. This carpenter ant was a burgundy color and quite small in size. Compared to the other ants crawling around it the size was quite small but the burgundy stood out. It had hints of black ( the antenna and other small features).<\/li>\n<\/ul>\n",Hymenoptera,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/176A9CB0-FB6C-4499-A663-995FDF4E563C.jpeg,","
- Small, dark brown, rested at bottom of microcentrifuge tube<\/li>\n<\/ul>\n",No,,,,
"",37.0229000000,76.3452000000,04/12/2022,"
- Something may have went wrong during DNA extraction<\/li>\n<\/ul>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/2E05DC0E-595B-49BD-9D3E-217220B5AAEF-scaled.jpeg,",37.10546196574304,-76.48133139791112,04/05/2022,"
![\"\"](\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2022\/02\/PHOTO-2022-02-23-12-54-31-225x300.jpg\")
<\/p>\n",Culicidae,Instagene Matrix,Whole arthropod,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/IMG_4560-scaled.jpg,",1.4254250000,103.7753330000,02/23/2022,"",Drosophila melanogaster,Instagene Matrix,Whole arthropod,,PBS,,"","",Yes,708 bp,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/IMG-7479-1-scaled.jpg,",,,,"",,,,,,,"","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/IMG-7478-scaled.jpg,",,,02/23/2022,"",,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/Screenshot-2022-02-25-at-8.54.49-AM.png,",,,02/23/2022,"",,Instagene Matrix,Abdomen,MiniPCR,PBS,,"","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/20220223_125544-scaled.jpg,",,,,"",,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/IMG-1677-scaled.jpg,",,,,"",,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/IMG-7388-scaled.jpg,",,,,"",,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/Screenshot-2022-02-25-at-8.52.06-AM.png,",103.7755,1.4258,02/23/2022,"",,,,,,,"","",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/Screenshot-2022-02-24-at-11.50.17-PM.png,",1.4257977,103.7755,02/23/2022,"",Diptera,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/Screenshot-2022-02-24-at-11.51.48-PM.png,",1.4257977,103.7755,02/23/2022,"",Diptera,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/Screenshot-2022-02-23-at-2.47.31-PM.png,",1.4259693082,103.7754141693,02/23/2022,"",Diptera,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/Screenshot-2022-02-24-at-11.43.25-PM.png,",1.4257977,103.7755,02/23/2022,"",Hymenoptera,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/IMG-5172-scaled.jpg,",1.4257977000,103.7755000000,02/24/2022,"",Endopterygota,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/IMG-5176-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/IMG-5174-scaled.jpg,",1.4257977000,103.7755000000,02/24/2022,"",Hexapoda,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/IMG_4717-1-scaled.jpg,",1.4302185000,103.7640034000,02/24/2022,"![\"\"](\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2022\/02\/IMG_9788.heic\")
<\/a><\/p>\n",Hexapoda,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/IMG_9348-scaled.jpg,",,,,"",,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/IMG_9356-scaled.jpg,",,,,"",,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/IMG_9362-scaled.jpg,",,,,"",,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/fly-1.jpg,",1.4257654,103.7754619,02/23/2022,"",Diptera,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/Snapchat-1483566675-1-scaled.jpg,",1.4258,103.7355,02/23/2022,"",,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/ant-2.jpg,",1.4257654,103.7754619,02/23/2022,"",Hymenoptera,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/Snapchat-755128865-scaled.jpg,",1.4258,103.7355,02/23/2022,"",,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/ant-1.jpg,",1.4257654000,103.7754619000,02/23/2022,"",Hymenoptera,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/Snapchat-498551139-scaled.jpg,",1.4258,103.7355,02/23/2022,"",,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/IMG_9354-scaled.jpeg,",1.4257977000,103.7755000000,02/23/2022,"",,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/Snapchat-776549737-scaled.jpg,",1.4258000000,103.7355000000,02/23/2022,"",Rhaphidophoridae,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/32F7BC32-798E-4EF9-BC3F-929C90ED325B.png,",1.4257977,103.7755,02/23/2022,"",Hexapoda,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/IMG_7350-scaled.jpg,",1.4258180000,103.7739299000,02/23/2022,"",Insecta,Instagene Matrix,Abdomen,MiniPCR,PBS,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/Screenshot-2022-03-11-at-9.11.06-AM.png,","",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/IMG_7100.jpg,",1.4257977000,103.7755000000,02/23/2022,"",Hymenoptera,Instagene Matrix,Whole arthropod,Standard electrophoresis system,PBS,,"","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/IMG_0134-scaled.jpg,",1.4257977000,103.7755000000,02/23/2022,"![\"\"](\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2022\/01\/Screenshot-2022-01-28-12.41.22-PM-223x300.png\")
<\/p>\n![\"\"](\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2021\/08\/IMG-1714-300x225.jpg\")
<\/p>\n![\"\"](\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2021\/07\/EEBD2588-3DA4-4B3F-9A9C-C9601220E49E-225x300.jpeg\")
<\/p>\n",Insecta,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/07/image1-39-rotated.jpeg,",40.5682900000,-74.4173877000,06/11/2020,"![\"\"](\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2021\/07\/5A54A7F8-485F-4FE7-B011-D8D953994B77-225x300.jpeg\")
![\"\"](\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2021\/07\/74B38A9D-7BD8-4CEE-93B4-1E40791B21F0-225x300.jpeg\")
<\/p>\n",Hexapoda,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/07/image-5-scaled.jpg,",41.53696371487127,-72.08303840664567,07/06/2021,"![\"\"](\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2021\/04\/AD60BAA1-AE7A-4CB7-8B19-0D1677B17558-300x91.jpeg\")
<\/p>\n",Formicidae,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/79A9DD61-D1CA-43AF-9056-8F65856E9B7D.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/3D3D87C9-53B8-4997-A3EC-B7456995BF51.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/27659100-E049-432D-9C7D-E1ADC3469A59.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/2E9EF6D9-690C-4E83-9EEF-ADB740936281.jpeg,","![\"\"](\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2021\/04\/Screenshot-2021-04-08-at-8.17.51-AM-300x143.png\")
<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/IMG_5054-scaled.jpg,",41.7081094000,-88.1945129000,10/04/2020,"![\"\"](\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2021\/04\/Ameise-1-225x300.jpg\")
<\/p>\n",Lasius niger,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Gel.png,","![\"\"](\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2021\/04\/Spinne-300x163.jpg\")
<\/p>\n",Pardosa lugubris,Edwards Buffer,Partial abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Gel.png,","![\"\"](\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2020\/10\/pgarro-2-146x300.jpg\")
<\/p>\n![\"\"](\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2020\/10\/Mhu2insect-225x300.jpg\")
<\/p>\n