# This file is a CSV export of the The Wolbachia Project database on Feb 07 2025 15:21:29 GMT image_urls,location_lat,location_lon,collection_date,observations,putative_identification,dna_extraction_kit,dna_extraction_location,gel_rig,running_buffer,dna_stain,gel_image_urls,protocol_notes,wolbachia_presence,wolbachia_16s_sequence,wolbachia_16s_fasta,wolbachia_16s_ab1,arthropod_COI_sequence,arthropod_COI_fasta,arthropod_COI_ab1, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Ahpids14Jan2025_2-1.jpg,",40.2927851000,-76.8834460000,01/14/2025,"

Moving around on plant<\/p>\n

Eating plant material<\/p>\n",Myzus persicae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,Other,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Ahpids14Jan2025_1-2.jpg,",40.2927851000,-76.8834460000,01/14/2025,"

Moving around on plant<\/p>\n

Eating plant material<\/p>\n",Myzus persicae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,Other,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Ahpids14Jan2025_1-1.jpg,",40.2926972480,-76.8833433400,01/14/2025,"

moving around on plant.<\/p>\n

eating plant material.<\/p>\n",Myzus persicae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,Other,SYBR Safe,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Ahpids14Jan2025_2.jpg,",40.2927851000,-76.8834460000,01/14/2025,"

Moving around on plant<\/p>\n

Eating plant material<\/p>\n",Myzus persicae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,Other,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/SingleAphid14Jan2025.jpg,",40.2926972480,-76.8833433400,01/14/2025,"

Moving around on plant<\/p>\n

Eating plant material<\/p>\n",Myzus persicae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,Other,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Ahpids14Jan2025_1.jpg,",40.2926972481,-76.8833433421,01/14/2025,"

Moving around on plant.<\/p>\n

Eating plant material.<\/p>\n",Myzus persicae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,Other,SYBR Safe,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/WhatsApp-Image-2025-02-04-at-10.23.23.jpeg,",32.00350659976398,34.77098596032163,12/25/2024,"

Caught on the wall<\/p>\n",Diptera,DNeasy (Qiagen),Whole arthropod,MiniOne,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/geli.jpg,","

1. Caught the bug with our hands
\n2. Place it in a Ethanol-filled test tube
\n3. DNA extraction process
\n4. DNA replication using PCR machine
\n5. electrophoresis<\/p>\n",Yes,,,, "",32.0029540240,34.7698122131,12/25/2024,"

It was alive found on a wall<\/p>\n",Arthropoda,DNeasy (Qiagen),Whole arthropod,MiniOne,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/WhatsApp.jpeg,","

Caught the spider with our hands
\nPlace it in a Ethanol-filled test tube
\nDNA extraction process
\nDNA replication using PCR machine
\nelectrophoresis<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/נמלה-וולבכיה.jpeg,",32.1454080000,34.9077504000,12/25/2024,"

We found an ant, in school yard.<\/p>\n",Messor ebeninus,DNeasy (Qiagen),Whole arthropod,,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/gel-1-1.jpeg,","

    \n
  1. Caught the ant with our hands<\/li>\n
  2. Place it in a Ethanol-filled test tube<\/li>\n
  3. DNA extraction process<\/li>\n
  4. DNA replication using PCR machine<\/li>\n
  5. electrophoresis<\/li>\n<\/ol>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/harak-no-wolbachia-1.jpg,",32.003491779,34.7710243612,12/25/2024,"

    Caught on a leaf of a big bush.<\/p><\/blockquote>\n",Oncopeltus fasciatus,DNeasy (Qiagen),Whole arthropod,MiniOne,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/1000098710.jpg,","

      \n
    1. Caught the bug with our hands<\/li>\n
    2. Place it in a Ethanol-filled test tube<\/li>\n
    3. DNA extraction process<\/li>\n
    4. DNA replication using PCR machine<\/li>\n
    5. electrophoresis<\/li>\n<\/ol>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_2235-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_2246-scaled.jpg,",42.4836790000,-71.1423155000,09/26/2024,"

      Green, small, and round. Tan legs.<\/p>\n",Harpalus rufipes,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_3059-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_3060-scaled.jpg,","

      Wolbachia experiment lab 1 to 2 to 3 to 4 followed as stated.<\/p>\n

      1% agarose gel was 0.4g of agarose in 40 mL of buffer.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/unnamed-1-scaled.jpg,",42.4836790000,-71.1423155000,09/26/2024,"

      The Grub was found under a Rock and was\u00a0 a really white and some what see through<\/p>\n",Phyllophaga,DNeasy (Qiagen) blood and tissue kit,,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-31-at-12.46.47 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-31-at-12.46.53 PM.png,","

      wolbachia lab 1 then followed to lab 2 then to lab 3 then to lab 4. the only thing changed was the amout of agrose gel which was change to 40 ML<\/p>\n",No,,,, "",44.8662492000,-68.7906169000,11/07/2024,"

      The Japanese Lady Bug was small with a hard outer layer covering its abdomen. I extracted the abdomen of the lady bug for my PCR test.<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_5981.jpg,","

      During my lab procedure everything went as planned, there was no contamination and we got the expected results. The only problem that occurred was that we ran out of positive DNA for our 5th well so we didn’t get a positive DNA result.<\/p>\n",No,,,, "",44.8342071582,-68.7727224650,11/07/2025,"

      dead, very long legs, body and head seem to be as one.<\/p>\n",Opiliones,DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL,Abdomen,Standard electrophoresis system,TBE,Other,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_4651-scaled.jpeg,",44.8016128000,-68.7712257000,11/07/2024,"",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_5254-scaled.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/20241107_083600-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/20241107_083538-scaled.jpg,",44.8662492000,-68.7906169000,11/07/2024,"",Lepidoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/1000012404-scaled.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_0256-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_0184-scaled.jpeg,",44.8662492000,-68.7906169000,11/07/2025,"

      You can see a part of the Wasps gametes from the arthropods abdomen.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"","

      I did not end up running the gel electrophoresis. This is because there was an accidental mix of the taskmaster mix with the overall total DNA gathered from the arthropod abdomen. This ended up ruining the results for the gel electrophoresis.<\/p>\n",,,,, "",44.8656860329,-68.7914984395,11/07/2024,"",Coccinellidae,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_8234.jpg,","

      My results were in the wells 2-7.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/1000006065-scaled.jpg,",,,11/07/2025,"",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_0376.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6625-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6626-scaled.jpeg,",44.8646708854,-68.7375178401,11/07/2024,"",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-31-at-9.49.10 AM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6629-scaled.jpeg,",44.8019983578,-68.7695226057,11/07/2024,"",Araneae,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_4357-scaled.jpeg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_3522-1.jpg,",,,11/07/2024,"

      Black Wasp.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_3736-scaled.jpeg,",44.8053122000,-68.7685691000,08/31/2024,"",Dermaptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_4278-scaled.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/spiders1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/spiders2-scaled.jpg,",44.8662492000,-68.7906169000,10/15/2024,"",Araneae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/0114250955-scaled.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-06-at-10.03.06 AM.png,",44.8662492000,-68.7906169000,11/07/2024,"

      Black Fly<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_8234.jpg,","

      The results for the Black Fly are in the second set of wells titled 1-6.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6730-1-1-scaled.jpg,",42.4835021188,-71.1422491564,09/26/2024,"",Dissosteira carolina,DNeasy (Qiagen) blood and tissue kit,Gonads dissected,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_7020-2.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_7022-2.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_0502-scaled.jpeg,",44.9333081013,-93.1369187270,09/10/2024,"

      I found this ant in an Urban environment, in the city of St. Paul coming into resident’s houses. This occurred during September as summer was transitioning into fall. There have been many flying ants coming into my house, but I only collected one. It was around 70 degrees Fahrenheit.<\/p>\n",Camponotus,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-11-155854.png,","

      Use SeeGreen DNA dye in 1.5% aragose gel in TBE buffer<\/p>\n

      MiniPCR Electrophoresis system<\/p>\n

      5\u00b5l DNA ladder<\/p>\n

      2\u00b5 loading dye + 8\u00b5 DNA in each lane<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_5851-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_5852-scaled.jpg,",44.9510901582,-93.1046471203,08/23/2024,"

      It was caught on a tree. it has brown legs and a white body.<\/p>\n",Araneae,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/unnamed-scaled.jpg,",44.9314816000,-93.1692544000,08/23/2024,"

      Lots of legs, Brown, Small<\/p>\n",Arthropoda,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_5338.jpg,",44.94794749981588,-93.0912514144291,,"

      it was late summer\/ early fall<\/p>\n

      found in the grass next to a tree<\/p>\n",Hymenoptera,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_3341-scaled.jpeg,",,,03/04/2025,"

      Very small, brown\/red, color 6 legs one leg is damaged<\/p>\n",Hymenoptera,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_1075-scaled.jpg,",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_4812-scaled.jpeg,",36.1444896000,-86.8011462000,,"",Hexapoda,,,,,,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-08-125656.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-08-125716.png,",44.9314816000,-93.1692544000,08/23/2025,"

      light and dark brown<\/p>\n

      has legs<\/p>\n

      reflective pattern down its back<\/p>\n",Araneae,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/wolbach-scaled.jpg,",44.9259166,-93.17279579999999,08/08/2024,"

      Large grey-orange thorax, orange legs, head, and abdomen.<\/p>\n",Araneae,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Rolly-Polly.png,",44.971274229,-93.2813637912,08/22/2024,"

      found in the dirt outside of school. curled into a ball the second I\u00a0 picked it up.<\/p>\n",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_9504-scaled.jpg,",44.928516549024486,-93.17056562749528,08/22/2024,"

      I found this Ant climbing on a tree in my schools yard. I collected it in the afternoon, around 2:00 PM, and it was a sunny day. There were other ants near the area which I collected this one. \"\"<\/p>\n",Hymenoptera,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-07-142318-2.png,",44.9314816000,-93.1692544000,08/22/2024,"

      Round, Grey, stubby, little segments on the back.<\/p>\n",Armadillidium vulgare,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_0053-scaled.jpg,",44.9265103390,-93.1725936057,,"

      I found this Rolly Polly in the grass near a bush at my High-School St. Paul Acadamy. I found roughly 2 pm in the Afternoon on August 22. It was about 74 degrees outside. There were multiple other Rolly Polly and other bugs around the area I found my Rolly Polly.<\/p>\n",Armadillidae,,,,,,"","",,,,, "",42.5920977000,-72.6033351000,09/14/2024,"

      Collected\u00a0 outside after it died<\/p>\n",Hymenoptera,DNeasy (Qiagen),,MiniOne,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/GD-Wolbachia.png,","

      Followed Wolbachia Lab Protocol for students<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/20241106_090211-1-scaled.jpg,",42.5934203575,-72.5932735058,09/14/2024,"",Hymenoptera,DNeasy (Qiagen),Rear half,MiniOne,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/JJ-Wolbachia-Results.png,","

      Followed Wolbachia Project Lab Protocol<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_0155-scaled.jpg,",44.9264298435,-93.1728042215,08/22/2025,"

      Brown, small, oval, legs<\/p>\n",Arthropoda,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_0743-rotated.jpg,",44.9261630074,-93.1754939783,08/22/2024,"

      scaley, round, lots of legs,\u00a0 and small.<\/p>\n",Arthropoda,,,,,,"","",,,,, "",44.9259606380,-93.1732330101,03/02/2025,"

      \"\"<\/p>\n",Armadillidium,,,,,,"","",,,,, "",44.9537029000,-93.0899578000,08/23/2024,"

      Small, dark<\/p>\n

      six legs, two antenea<\/p>\n

      two joints in each leg<\/p>\n

      clear separation of head, abdomen, thorax<\/p>\n

      head tapers into two pincers<\/p>\n",Hymenoptera,,,,,,"","",,,,, "",,,08/23/2024,"

      Small and grey<\/p>\n",Arthropoda,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Bio-Bugs-scaled-e1736268791378.jpg,",44.9314816000,-93.1692544000,,"

      Exoskeleton, six legs, head, thorax, abdomen<\/p>\n",Formicidae,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Image-8-scaled.jpeg,",47.4901117000,8.5036780000,08/24/2024,"

      caught in kitchen<\/p>\n",Vespula germanica,Instagene Matrix,Rear half,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Image-1.png,","

      The slots were placed at the wrong place, and there wasn’t enough blue tracing dye in the samples.<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/22_Arthropod-CO1F.fasta,, "",45.4762586687,-122.7580061590,11/19/2024,"

      Crawling, clinging to reeds, rapid movement, folded in half when collected.<\/span> Large shield-backed, yellow-blackish colored.<\/span> Many were in same area, all behaved very similarly.\u00a0<\/span><\/p>\n",Hesperocorixa laevigata,,,,,,"","",,,,, "",45.4760262659,-122.7581292374,11/11/2024,"

      Collected in wetlands, on edge of pond. Clinging to foliage, fast-moving. Contains head, segmented thorax, abdomen, 8 legs, antennae, but no wings.<\/p>\n",Odonata,,,,,,"","",,,,, "",45.4395124,-122.7711943,11/20/2024,"

      Observations and Comments: crysalises were a shade between beige and white colored, and they had small holes in them all over the outer covering, they kind of looked like a seashell with ridged edges<\/b><\/p>\n",Arthropoda,,,,,,"","",Unknown,,,, "",45.474009,-122.75507,11/20/2024,"

      Fly was very small, much smaller than the average house fly. It flew around very fast and was quite hard to catch. <\/b><\/p>\n",Arthropoda,,,,,,"","",Unknown,,,, "",45.4775021520,-122.7587600102,11/20/2024,"