# This file is a CSV export of the The Wolbachia Project database on Feb 07 2025 15:21:29 GMT image_urls,location_lat,location_lon,collection_date,observations,putative_identification,dna_extraction_kit,dna_extraction_location,gel_rig,running_buffer,dna_stain,gel_image_urls,protocol_notes,wolbachia_presence,wolbachia_16s_sequence,wolbachia_16s_fasta,wolbachia_16s_ab1,arthropod_COI_sequence,arthropod_COI_fasta,arthropod_COI_ab1, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Ahpids14Jan2025_2-1.jpg,",40.2927851000,-76.8834460000,01/14/2025,"
Moving around on plant<\/p>\n
Eating plant material<\/p>\n",Myzus persicae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,Other,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Ahpids14Jan2025_1-2.jpg,",40.2927851000,-76.8834460000,01/14/2025,"
Moving around on plant<\/p>\n
Eating plant material<\/p>\n",Myzus persicae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,Other,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Ahpids14Jan2025_1-1.jpg,",40.2926972480,-76.8833433400,01/14/2025,"
moving around on plant.<\/p>\n
eating plant material.<\/p>\n",Myzus persicae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,Other,SYBR Safe,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Ahpids14Jan2025_2.jpg,",40.2927851000,-76.8834460000,01/14/2025,"
Moving around on plant<\/p>\n
Eating plant material<\/p>\n",Myzus persicae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,Other,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/SingleAphid14Jan2025.jpg,",40.2926972480,-76.8833433400,01/14/2025,"
Moving around on plant<\/p>\n
Eating plant material<\/p>\n",Myzus persicae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,Other,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Ahpids14Jan2025_1.jpg,",40.2926972481,-76.8833433421,01/14/2025,"
Moving around on plant.<\/p>\n
Eating plant material.<\/p>\n",Myzus persicae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,Other,SYBR Safe,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/WhatsApp-Image-2025-02-04-at-10.23.23.jpeg,",32.00350659976398,34.77098596032163,12/25/2024,"
Caught on the wall<\/p>\n",Diptera,DNeasy (Qiagen),Whole arthropod,MiniOne,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/geli.jpg,","
1. Caught the bug with our hands
\n2. Place it in a Ethanol-filled test tube
\n3. DNA extraction process
\n4. DNA replication using PCR machine
\n5. electrophoresis<\/p>\n",Yes,,,,
"",32.0029540240,34.7698122131,12/25/2024,"
It was alive found on a wall<\/p>\n",Arthropoda,DNeasy (Qiagen),Whole arthropod,MiniOne,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/WhatsApp.jpeg,","
Caught the spider with our hands
\nPlace it in a Ethanol-filled test tube
\nDNA extraction process
\nDNA replication using PCR machine
\nelectrophoresis<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/נמלה-וולבכיה.jpeg,",32.1454080000,34.9077504000,12/25/2024,"
We found an ant, in school yard.<\/p>\n",Messor ebeninus,DNeasy (Qiagen),Whole arthropod,,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/gel-1-1.jpeg,","
Caught on a leaf of a big bush.<\/p><\/blockquote>\n",Oncopeltus fasciatus,DNeasy (Qiagen),Whole arthropod,MiniOne,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/1000098710.jpg,","
\n
- Caught the bug with our hands<\/li>\n
- Place it in a Ethanol-filled test tube<\/li>\n
- DNA extraction process<\/li>\n
- DNA replication using PCR machine<\/li>\n
- electrophoresis<\/li>\n<\/ol>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_2235-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_2246-scaled.jpg,",42.4836790000,-71.1423155000,09/26/2024,"
Green, small, and round. Tan legs.<\/p>\n",Harpalus rufipes,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_3059-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_3060-scaled.jpg,","
Wolbachia experiment lab 1 to 2 to 3 to 4 followed as stated.<\/p>\n
1% agarose gel was 0.4g of agarose in 40 mL of buffer.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/unnamed-1-scaled.jpg,",42.4836790000,-71.1423155000,09/26/2024,"
The Grub was found under a Rock and was\u00a0 a really white and some what see through<\/p>\n",Phyllophaga,DNeasy (Qiagen) blood and tissue kit,,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-31-at-12.46.47 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-31-at-12.46.53 PM.png,","
wolbachia lab 1 then followed to lab 2 then to lab 3 then to lab 4. the only thing changed was the amout of agrose gel which was change to 40 ML<\/p>\n",No,,,, "",44.8662492000,-68.7906169000,11/07/2024,"
The Japanese Lady Bug was small with a hard outer layer covering its abdomen. I extracted the abdomen of the lady bug for my PCR test.<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_5981.jpg,","
During my lab procedure everything went as planned, there was no contamination and we got the expected results. The only problem that occurred was that we ran out of positive DNA for our 5th well so we didn’t get a positive DNA result.<\/p>\n",No,,,, "",44.8342071582,-68.7727224650,11/07/2025,"
dead, very long legs, body and head seem to be as one.<\/p>\n",Opiliones,DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL,Abdomen,Standard electrophoresis system,TBE,Other,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_4651-scaled.jpeg,",44.8016128000,-68.7712257000,11/07/2024,"",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_5254-scaled.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/20241107_083600-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/20241107_083538-scaled.jpg,",44.8662492000,-68.7906169000,11/07/2024,"",Lepidoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/1000012404-scaled.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_0256-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_0184-scaled.jpeg,",44.8662492000,-68.7906169000,11/07/2025,"
You can see a part of the Wasps gametes from the arthropods abdomen.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"","
I did not end up running the gel electrophoresis. This is because there was an accidental mix of the taskmaster mix with the overall total DNA gathered from the arthropod abdomen. This ended up ruining the results for the gel electrophoresis.<\/p>\n",,,,, "",44.8656860329,-68.7914984395,11/07/2024,"",Coccinellidae,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_8234.jpg,","
My results were in the wells 2-7.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/1000006065-scaled.jpg,",,,11/07/2025,"",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_0376.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6625-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6626-scaled.jpeg,",44.8646708854,-68.7375178401,11/07/2024,"",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-31-at-9.49.10 AM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6629-scaled.jpeg,",44.8019983578,-68.7695226057,11/07/2024,"",Araneae,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_4357-scaled.jpeg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_3522-1.jpg,",,,11/07/2024,"
Black Wasp.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_3736-scaled.jpeg,",44.8053122000,-68.7685691000,08/31/2024,"",Dermaptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_4278-scaled.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/spiders1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/spiders2-scaled.jpg,",44.8662492000,-68.7906169000,10/15/2024,"",Araneae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/0114250955-scaled.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-06-at-10.03.06 AM.png,",44.8662492000,-68.7906169000,11/07/2024,"
Black Fly<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_8234.jpg,","
The results for the Black Fly are in the second set of wells titled 1-6.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6730-1-1-scaled.jpg,",42.4835021188,-71.1422491564,09/26/2024,"",Dissosteira carolina,DNeasy (Qiagen) blood and tissue kit,Gonads dissected,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_7020-2.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_7022-2.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_0502-scaled.jpeg,",44.9333081013,-93.1369187270,09/10/2024,"
I found this ant in an Urban environment, in the city of St. Paul coming into resident’s houses. This occurred during September as summer was transitioning into fall. There have been many flying ants coming into my house, but I only collected one. It was around 70 degrees Fahrenheit.<\/p>\n",Camponotus,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-11-155854.png,","
Use SeeGreen DNA dye in 1.5% aragose gel in TBE buffer<\/p>\n
MiniPCR Electrophoresis system<\/p>\n
5\u00b5l DNA ladder<\/p>\n
2\u00b5 loading dye + 8\u00b5 DNA in each lane<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_5851-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_5852-scaled.jpg,",44.9510901582,-93.1046471203,08/23/2024,"
It was caught on a tree. it has brown legs and a white body.<\/p>\n",Araneae,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/unnamed-scaled.jpg,",44.9314816000,-93.1692544000,08/23/2024,"
Lots of legs, Brown, Small<\/p>\n",Arthropoda,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_5338.jpg,",44.94794749981588,-93.0912514144291,,"
it was late summer\/ early fall<\/p>\n
found in the grass next to a tree<\/p>\n",Hymenoptera,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_3341-scaled.jpeg,",,,03/04/2025,"
Very small, brown\/red, color 6 legs one leg is damaged<\/p>\n",Hymenoptera,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_1075-scaled.jpg,",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_4812-scaled.jpeg,",36.1444896000,-86.8011462000,,"",Hexapoda,,,,,,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-08-125656.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-08-125716.png,",44.9314816000,-93.1692544000,08/23/2025,"
light and dark brown<\/p>\n
has legs<\/p>\n
reflective pattern down its back<\/p>\n",Araneae,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/wolbach-scaled.jpg,",44.9259166,-93.17279579999999,08/08/2024,"
Large grey-orange thorax, orange legs, head, and abdomen.<\/p>\n",Araneae,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Rolly-Polly.png,",44.971274229,-93.2813637912,08/22/2024,"
found in the dirt outside of school. curled into a ball the second I\u00a0 picked it up.<\/p>\n",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_9504-scaled.jpg,",44.928516549024486,-93.17056562749528,08/22/2024,"
I found this Ant climbing on a tree in my schools yard. I collected it in the afternoon, around 2:00 PM, and it was a sunny day. There were other ants near the area which I collected this one.
<\/p>\n",Hymenoptera,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-07-142318-2.png,",44.9314816000,-93.1692544000,08/22/2024,"
Round, Grey, stubby, little segments on the back.<\/p>\n",Armadillidium vulgare,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_0053-scaled.jpg,",44.9265103390,-93.1725936057,,"
I found this Rolly Polly in the grass near a bush at my High-School St. Paul Acadamy. I found roughly 2 pm in the Afternoon on August 22. It was about 74 degrees outside. There were multiple other Rolly Polly and other bugs around the area I found my Rolly Polly.<\/p>\n",Armadillidae,,,,,,"","",,,,, "",42.5920977000,-72.6033351000,09/14/2024,"
Collected\u00a0 outside after it died<\/p>\n",Hymenoptera,DNeasy (Qiagen),,MiniOne,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/GD-Wolbachia.png,","
Followed Wolbachia Lab Protocol for students<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/20241106_090211-1-scaled.jpg,",42.5934203575,-72.5932735058,09/14/2024,"",Hymenoptera,DNeasy (Qiagen),Rear half,MiniOne,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/JJ-Wolbachia-Results.png,","
Followed Wolbachia Project Lab Protocol<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_0155-scaled.jpg,",44.9264298435,-93.1728042215,08/22/2025,"
Brown, small, oval, legs<\/p>\n",Arthropoda,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_0743-rotated.jpg,",44.9261630074,-93.1754939783,08/22/2024,"
scaley, round, lots of legs,\u00a0 and small.<\/p>\n",Arthropoda,,,,,,"","",,,,, "",44.9259606380,-93.1732330101,03/02/2025,"
<\/p>\n",Armadillidium,,,,,,"","",,,,, "",44.9537029000,-93.0899578000,08/23/2024,"
Small, dark<\/p>\n
six legs, two antenea<\/p>\n
two joints in each leg<\/p>\n
clear separation of head, abdomen, thorax<\/p>\n
head tapers into two pincers<\/p>\n",Hymenoptera,,,,,,"","",,,,, "",,,08/23/2024,"
Small and grey<\/p>\n",Arthropoda,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Bio-Bugs-scaled-e1736268791378.jpg,",44.9314816000,-93.1692544000,,"
Exoskeleton, six legs, head, thorax, abdomen<\/p>\n",Formicidae,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Image-8-scaled.jpeg,",47.4901117000,8.5036780000,08/24/2024,"
caught in kitchen<\/p>\n",Vespula germanica,Instagene Matrix,Rear half,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Image-1.png,","
The slots were placed at the wrong place, and there wasn’t enough blue tracing dye in the samples.<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/22_Arthropod-CO1F.fasta,, "",45.4762586687,-122.7580061590,11/19/2024,"
Crawling, clinging to reeds, rapid movement, folded in half when collected.<\/span> Large shield-backed, yellow-blackish colored.<\/span> Many were in same area, all behaved very similarly.\u00a0<\/span><\/p>\n",Hesperocorixa laevigata,,,,,,"","",,,,, "",45.4760262659,-122.7581292374,11/11/2024,"
Collected in wetlands, on edge of pond. Clinging to foliage, fast-moving. Contains head, segmented thorax, abdomen, 8 legs, antennae, but no wings.<\/p>\n",Odonata,,,,,,"","",,,,, "",45.4395124,-122.7711943,11/20/2024,"
Observations and Comments: crysalises were a shade between beige and white colored, and they had small holes in them all over the outer covering, they kind of looked like a seashell with ridged edges<\/b><\/p>\n",Arthropoda,,,,,,"","",Unknown,,,, "",45.474009,-122.75507,11/20/2024,"
Fly was very small, much smaller than the average house fly. It flew around very fast and was quite hard to catch. <\/b><\/p>\n",Arthropoda,,,,,,"","",Unknown,,,, "",45.4775021520,-122.7587600102,11/20/2024,"
\n
- Location Description: <\/b>The arthropod was found under tree bark in the wetlands at Oregon Episcopal School.<\/span><\/li>\n
- Season: <\/b>Autumn<\/span><\/li>\n
- Temperature: <\/b>51\u00ba<\/span><\/li>\n
- Habitat:<\/b> Terrestrial<\/span><\/li>\n
- Behavioral Observations: <\/b>Upon uncovering, the arthropod quickly moved toward surrounding tree bark.\u00a0<\/span><\/li>\n
- Physical Description:<\/b>\n
\n
- Number of legs: 28<\/span><\/li>\n
- Presence of wings: No<\/span><\/li>\n
- Presence of antennae: Yes<\/span><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n",Chilopoda,,,,,,"","",,,,, "",45.4963538000,-122.7216995000,11/17/2024,"
<\/p>\n
\n
- Location Description:<\/b> The arthropod was found on the surface of a domesticated dog.<\/span><\/li>\n
- Season: <\/b>Autumn<\/span><\/li>\n
- Temperature: <\/b>71\u00ba<\/span><\/li>\n
- Habitat: <\/b>Other<\/span><\/li>\n
- Behavioral Observations: <\/b>Moved quickly and attempted to flee.<\/span><\/li>\n
- Body segmentation (head, thorax, abdomen)<\/span><\/li>\n
- Number of legs: 6<\/span><\/li>\n
- Presence of wings: no<\/span><\/li>\n
- Presence of antennae: yes<\/span><\/li>\n<\/ul>\n",Ctenocephalides felis,,,,,,"","",,,,, "",45.4740108660,-122.7545311973,11/20/2024,"
Very small, crawled very quickly around the tube and collection area, hard to place in collection tube<\/p>\n",Arachnida,,,,,,"","",,,,, "",45.4737847565,-122.7548192176,11/20/2024,"
We found this bug on the curtain of the school nurse’s office between 8:30 and 9:30 a.m. It was very cold outside but the bug was inside at around room temperature, so maybe around 75 degrees Fahrenheit.<\/p>\n",Leptoglossus occidentalis,,,,,,"","",,,,, "",45.4742068007,-122.7568815983,11/20/2024,"
I found it below the dead log around 11:30 am. The temperature was about 10.5 C. The log was placed on many wet leaves.<\/p>\n",Armadillidium vulgare,,,,,,"","",,,,, "",45.47411,-122.75458,11/20/2024,"
\n
- Body segmentation: Head, thorax, abdomen<\/span><\/li>\n
- Number of legs: 6<\/span><\/li>\n
- Presence of wings: Yes<\/span><\/li>\n
- Presence of antennae: Yes<\/span><\/li>\n<\/ul>\n",Diptera,,,,,,"","",,,,, "",45.4772800000,-122.7582200000,11/20/2024,"
On the shore by the OES Wetlands on some leaves.<\/p>\n
51\u00ba F<\/p>\n
\n
- Body segmentation (e.g., head, thorax, abdomen): head<\/span><\/li>\n
- Number of legs: 6<\/span><\/li>\n
- Presence of wings: Yes<\/span><\/li>\n
- Presence of antennae: Yes<\/span><\/li>\n<\/ul>\n
<\/p>\n",Diptera,,,,,,"","",,,,, "",45.4739332879,-122.7542453896,11/20/2024,"
This Bark Centipede was collected next to a greenhouse in the soil beneath some concrete blocks. The soil was moist and surrounded by grass and overgrown plants. The specimen was collected at around noon with a temperature of 10.6 \u2103 at the tail end of fall. Arthropods were scarce at this time of day\/year and only this centipede was beneath the chosen stone.<\/span><\/p>\n",Cryptopidae,DNeasy (Qiagen),Abdomen,,,,"","",,,,, "",45.4739332870,-122.7542453000,11/20/2024,"
This Bark Centipede was collected next to a greenhouse in the soil beneath some concrete blocks. The soil was moist and surrounded by grass and overgrown plants. The specimen was collected at around noon with a temperature of 10.6 \u2103 at the tail end of fall. Arthropods were scarce at this time of day\/year and only this centipede was beneath the chosen stone.<\/span><\/p>\n",Cryptopidae,DNeasy (Qiagen),Abdomen,,,,"","",,,,, "",45.4740577,-122.7553359,11/20/2024,"
Behavioral Observations:<\/b><\/p>\n
Very fast with rapid movements. It almost avoided the light when the tree bark was pulled back.<\/p>\n
Location:\u00a0<\/strong><\/p>\n
Found in Oregon Episcopal School wetlands under the bark of a tree branch.<\/p>\n
Physical Description:<\/b><\/p>\n
\n
- \n
\n
- Body segmentation (e.g., head, thorax, abdomen)<\/span><\/li>\n
- Number of legs: 34<\/span><\/li>\n
- Presence of wings: No<\/span><\/li>\n
- Presence of antennae: Yes<\/span><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n",Myriapoda,,,,,,"","",,,,, "",45.4740483000,-122.7553890000,11/20/2024,"
Observations:<\/b><\/p>\n
Behavioral Observations:<\/strong><\/p>\n
\n
- Scurried across the branch when the bark of the tree was pulled back<\/li>\n<\/ul>\n
Location:<\/strong><\/p>\n
\n
- Found in the Oregon Episcopal School wetlands under the bark of a tree branch (51\u00ba F)<\/li>\n<\/ul>\n
Physical Description:<\/b><\/p>\n
\n
- Body segmentation (e.g., head, thorax, abdomen) 12 different body segments from head to tail<\/span><\/li>\n
- Number of legs: 14<\/span><\/li>\n
- Presence of wings: No<\/span><\/li>\n
- Presence of antennae: Yes<\/span><\/li>\n
- <\/li>\n<\/ul>\n",Armadillidae,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/3446AE1B-8149-4433-B5C8-4EC7927DFDE7.jpeg,",41.7756400000,-88.0970000000,09/10/2024,"
\n
- collected by Fall 2024 students<\/li>\n<\/ul>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","
\n\n<\/blockquote>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bug-11.png,",37.9703726261,-84.5043897515,09/16/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol.<\/p>\n
The specimen was identified as a Picture-winged Fly Delphinia using the iNaturalist app.<\/p>\n",Tephritidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bug-11-gel.png,","
GoTaqGreen<\/span> polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 <\/span>uL<\/span>. 5 <\/span>uL<\/span> of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes for the specimen are boxed in the gel picture.\u202f\u202f<\/span><\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bug-10.png,",37.9744322832,-84.5043897515,09/10/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol.<\/p>\n
The specimen was identified as a Mosquito using the iNaturalist app.<\/p>\n",Culicidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Mosquito-10-gel.png,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 uL. 5 uL of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes for the specimen are boxed in the gel picture.<\/p>\n",Yes,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Mosquito-1.png,",37.9725378045,-84.5030164604,09/08/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol.<\/p>\n
The specimen was identified as an Asian Tiger Mosquito using the iNaturalist app.<\/p>\n",Aedes,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Gel-mosquito-1.png,","
GoTaqGreen<\/span> polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 <\/span>uL<\/span>. 5 <\/span>uL<\/span> of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes for the specimen are boxed in the gel picture.\u202f\u202f<\/span><\/span>\u00a0<\/span><\/p>\n",Yes,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bug-18.png,",38.2097967000,-84.5588311000,,"
Caught in backyard of residence. The specimen was identified as a stink bug using the iNaturalist app.<\/p>\n",Pentatomidae,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Wolbachia-Project-PCR-results-bls-bug-18.png,","
GoTaqGreen<\/span> polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 <\/span>uL<\/span>. 5 <\/span>uL<\/span> of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes for the specimen are boxed in the gel picture.\u202f\u202f<\/span><\/span>\u00a0<\/span><\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bug-19.png,",38.2097967000,-84.5588311000,,"
Caught in backyard of residence. The specimen was identified as a stink bug using the iNaturalist app.<\/p>\n",Pentatomidae,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Wolbachia-Project-PCR-results-bls-bug-19-1.png,","
GoTaqGreen<\/span> polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 <\/span>uL<\/span>. 5 <\/span>uL<\/span> of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes for the specimen are boxed in the gel picture.\u202f\u202f<\/span><\/span>\u00a0<\/span><\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Mosquito-14.png,",37.9734321727,-84.5037561380,09/08/2024,"
The specimen was collected using a flying insect trap <\/span>containing<\/span> banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch<\/span> adjacent to<\/span> a small creek in a suburban backyard<\/span> for two days<\/span>.<\/span> The trap was cut open and <\/span>the (<\/span>predominantly <\/span>flying<\/span>)<\/span> insects were removed and preserved in 70% alcohol. <\/span><\/p>\n
The specimen was identified as a Mosquito using the iNaturalist app.<\/p>\n",Culicidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Gel-for-14.png,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 uL. 5 uL of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes are boxed in the gel picture.<\/p>\n",Yes,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Moth-16.png,",37.9722671607,-84.5019864922,09/09/2024,"
The specimen was collected using a flying insect trap <\/span>containing<\/span> banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch<\/span> adjacent to<\/span> a small creek in a suburban backyard<\/span> for two days<\/span>.<\/span> The trap was cut open and <\/span>the (<\/span>predominantly <\/span>flying<\/span>)<\/span> insects were removed and preserved in 70% alcohol.<\/span><\/p>\n
T<\/span>he specimen was <\/span>identified as a Moth – Lepidoptera <\/span><\/span>using the <\/span>iNaturalist<\/span> app.\u202f<\/span><\/span>\u00a0<\/span><\/p>\n",Lepidoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Gel-for-16.png,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 uL. 5 uL of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes for the specimen are boxed in the gel picture.<\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bee-15.png,",37.9725378045,-84.5023298149,09/10/2024,"
The specimen was collected using a flying insect trap <\/span>containing<\/span> banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch<\/span> adjacent to<\/span> a small creek in a suburban backyard<\/span> for two days<\/span>.<\/span> The trap was cut open and <\/span>the (<\/span>predominantly <\/span>flying<\/span>)<\/span> insects were removed and preserved in 70% alcohol.<\/span><\/p>\n
The specimen was identified as a Bee – Apoidea using the iNaturalist app.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Gel-for-15.png,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 uL. 5 uL of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes are boxed.<\/p>\n",Yes,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Drosophila-Fruit-Fly-13.png,",37.9709139267,-84.5030164604,09/08/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol. The trap was then placed on the ground for an additional three days capturing additional insects. These insects included both crawling and flying insects. These insects were removed and preserved in 70% alcohol.<\/p>\n
The specimen was identified as a Drosophila Fruit Fly using the iNaturalist app.<\/p>\n",Drosophila,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Gel-for-13.png,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 uL. 5 uL of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes for the specimen #13 are boxed in the gel picture.<\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/bug-17-.png,",38.1813561096,-84.6152681355,10/27/2024,"
Was collected at a window in my house using toilet paper to capture them without crushing them, then putting them in a Ziplock bag full isopropyl alcohol to preserve until DNA extraction proceeded.<\/p>\n
The insect was identified as Genus Halyomorpha using the iNaturalist app.<\/p>\n",Halyomorpha,Qiagen Blood and Tissue kit,Whole arthropod,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Wolbachia-Project-PCR-results-bls-bug-17-1.png,","
GoTaqGreen<\/span> polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). <\/span>The PCR reaction volume was 25 <\/span>uL<\/span>. 5 <\/span>uL<\/span> of each reaction was run on a 2% agarose gel.\u202f<\/span>The relevant lanes, lanes 2<\/span><\/span> and 3<\/span><\/span>, for the specimen (#17<\/span><\/span>) are boxed.\u202f\u202f<\/span><\/span><\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Bug-24-ed2.jpg,",37.9706432769,-84.5006132012,09/09/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard.<\/p>\n
The specimen was identified as a Stripe-horned Green Lacewing using the iNaturalist app.<\/p>\n",Chrysopidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Gel-Number-24-1.jpg,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 uL. 5 uL of each reaction was run on a 2% agarose gel. The relevant lanes, lanes 8 and 9, for the specimen (#24) are boxed.<\/p>\n",No,Sequencing not performed,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Bug-26-ed.jpg,",37.9716353487,-84.5010806309,09/10/2024,"
The specimen was discovered feeding on my leg. It was gently swatted and preserved in 70% alcohol until analysis.<\/p>\n
The specimen was identified as an Aedes mosquito<\/span><\/span> using the iNaturalist app.<\/p>\n",Aedes,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Gel-Number-26.jpg,","
GoTaqGreen polymerase was used for the PCR. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 uL. 5 uL of each reaction was run on a 2% agarose gel. The relevant lanes, lanes 4 and 5, for the specimen (#26) are boxed.<\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/mosquito-27-2.png,",37.9717258701,-84.5023298149,09/08/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard.<\/p>\n
The specimen was identified as an Aedes using the iNaturalist app.<\/p>\n",Aedes,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bug-27-gel.png,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for duplex reaction PCRs. The PCR reaction volume was 25 uL. 10 uL of each reaction was run on a 2% agarose gel. The relevant gel lane (lane 2) for the specimen #27 is boxed.<\/p>\n",Yes,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bee-28.png,",37.9709139267,-84.5030164604,09/16/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol. The trap was then placed on the ground for an additional three days capturing additional insects. These insects included both crawling and flying insects. These insects were removed and preserved in 70% alcohol.<\/span>\u00a0<\/span><\/p>\n
The specimen was identified as a <\/span>bee (Anthophila)<\/span>\u00a0using the iNaturalist app.\u202f<\/span>\u00a0<\/span><\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bug-28-gel.png,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for duplex reaction PCRs. The PCR reaction volume was 25 uL. 10 uL of each reaction was run on a 2% agarose gel. The relevant lane (lane 3), for the specimen #28 is boxed.<\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Earwig-29.png,",37.9725378045,-84.5040464287,09/16/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol. The trap was then placed on the ground for an additional three days capturing additional insects. These insects included both crawling and flying insects. These insects were removed and preserved in 70% alcohol.<\/p>\n
The specimen was identified as an earwig (Forficulinae) using the iNaturalist app.<\/p>\n",Forficulidae,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bug-29-gel.png,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for duplex reaction PCRs. The PCR reaction volume was 25 uL. 10 uL of each reaction was run on a 2% agarose gel. The relevant lane \u00a0(lane 4) for the specimen #29 is boxed.<\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Fruit-fly-30.png,",37.9719965159,-84.5037031060,09/08/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol. The trap was then placed on the ground for an additional three days capturing additional insects. These insects included both crawling and flying insects. These insects were removed and preserved in 70% alcohol.<\/p>\n
The specimen was identified as a fruit fly (Drosophila) using the iNaturalist app.<\/p>\n",Drosophila,Blood and Tissue kit,Whole arthropod,Standard electrophoresis system,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bug-30-gel.png,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for duplex reaction PCRs. The PCR reaction volume was 25 uL. 10 uL of each reaction was run on a 2% agarose gel. The relevant lane (lane 5) for the specimen #30 is boxed.<\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Slide3.jpg,",37.9690193572,-84.4872236138,09/09/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol. The trap was then placed on the ground for an additional three days capturing additional insects. These insects included both crawling and flying insects. These insects were removed and preserved in 70% alcohol.<\/span>\u00a0<\/span><\/p>\n
The specimen was identified as a Culicine Mosquito<\/span>\u00a0using the iNaturalist app.<\/span><\/p>\n",Aedes albopictus,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Sample-7-Culicine-Asian-Tiger-Mosquito.png,","
GoTaqGreen<\/span> polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). <\/span>The PCR reaction volume was 25 <\/span>uL<\/span>. 5 <\/span>uL<\/span> of each reaction was run on a 2% agarose gel.\u202f<\/span>The relevant lanes, lanes 7\u00a0<\/span><\/span> and 8<\/span><\/span>, for the specimen (#8<\/span><\/span>) are boxed.\u202f\u202f<\/span><\/span>