# This file is a CSV export of the The Wolbachia Project database on Oct 01 2023 13:05:09 GMT image_urls,location_lat,location_lon,collection_date,observations,putative_identification,dna_extraction_kit,dna_extraction_location,gel_rig,running_buffer,dna_stain,gel_image_urls,protocol_notes,wolbachia_presence,wolbachia_16s_sequence,wolbachia_16s_fasta,wolbachia_16s_ab1,arthropod_COI_sequence,arthropod_COI_fasta,arthropod_COI_ab1, "",36.0538915111,-86.5833909642,09/11/2023,"
Green, small deceased.<\/p>\n",Arachnida,,,,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/po-scaled.jpg,","
<\/p>\n",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/firefly-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/firefly-2-scaled.jpg,",36.0852187000,-86.7166632000,09/10/2023,"
The arthropod has a red head with a black spot, black wings, and 6 legs. The environment was a clear grassy yard.<\/p>\n",Photinus pyralis,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/thumbnail_IMG_0273.jpg,",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/IMG_1358-scaled.jpg,",36.0462412076,-86.5958324524,09/08/2023,"
I caught this arthropod at the Antioch High School baseball field. I collected it at about 3:00 pm, it was 82 degrees and sunny. This was the only spider I saw in the area.<\/p>\n",Parasteatoda,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/IMG_7672-scaled.jpeg,",36.0474517296,-86.5980068833,11/02/2023,"
I caught it around the school i was walking out and i was looking around for some arthropod and i saw him jumping so i caught him<\/p>\n",Orthoptera,,,,,,"","",Unknown,,,, "",36.047024974826634,-86.59832813870874,09/18/2023,"
The only thing that changed in the insect was that the orange color of its body was removed a little.<\/p>\n",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/IMG_6686-scaled.jpeg,",36.0531600000,-86.5931200000,09/09/2023,"
I found the ant in my back yard it was a hot day so it was under the dirt.<\/p>\n",Hymenoptera,,,,,,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/IMG-7596.jpg,",44.92585284433357,-93.17276185588206,08/28/2023,"
Shrimp-looking, grey\/brown, pinkish on the bottom, squishy looking<\/p>\n
also, the app kept trying to tell me that it was a beetle…???<\/p>\n",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/IMG-7594-rotated.jpg,",44.9299696172,93.1700000000,08/28/2023,"
Hard, shiny greyish shell, many legs, curled into ball<\/p>\n",Crinocheta,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/fly-1.jpg,",44.9259166000,-93.1727958000,08/28/2023,"
smooth back and spiky butt wings were translusent<\/p>\n",Lucilia sericata,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/IMG_4187-rotated.jpg,",44.93,93.17,,"
small, black, many legs<\/p>\n",Hymenoptera,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/IMG_4185-rotated.jpg,",44.9300000000,93.1700000000,08/28/2023,"
small, black, many legs<\/p>\n",Hymenoptera,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/IMG-5244-scaled.jpg,",44.9176696000,-93.1477239000,08/24/2023,"
Around<\/p>\n
black<\/p>\n
14 legs<\/p>\n
small<\/p>\n",Megacopta cribraria,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/WIN_20230828_12_55_59_Pro.jpg,",44.92641856785317,-93.17294656063044,08/28/2023,"
light grey colour ~.5 cm in length<\/p>\n",Isopoda,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/worm-1.jpg,",44.9459916000,-93.1549175000,08/28/2023,"
in the mulch near the bushes<\/p>\n",Tenebrio,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/F7DBD49C-0A51-4509-A365-067605244222-scaled.jpeg,",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/C0CEA927-6915-4192-8705-3D752BCFB983-scaled.jpeg,",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/IMG_2772-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/IMG_2775-scaled.jpeg,",37.8036204545,-122.4247808243,08/10/2023,"
It had a proboscus, slender legs, wings<\/p>\n",Culex pipiens,BABEC,Whole arthropod,MiniPCR,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/IMG_2850-scaled.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Screenshot-2023-08-29-at-2.34.05-PM.png,",,,08/28/2023,"
Shiny green insect with wings and red eyes<\/p>\n",Phasmatidae,none,Abdomen,MiniOne,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/Screenshot-2023-09-11-at-2.27.27-PM.png,","
Babec protocol<\/p>\n",Yes,,,, "",36.1180247000,-86.7769647000,10/30/2020,"
There was nothing out of the ordinary about these ants.<\/p>\n",Hexapoda,Edwards Buffer,Abdomen,Standard electrophoresis system,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/Screen-Shot-2020-11-04-at-6.43.11-PM.png,","",Yes,,,, "",36.1059132,-86.7768273,10/30/2020,"
There was nothing out of the ordinary about this ant. It was found in a sidewalk crack.<\/p>\n",Hexapoda,,,Standard electrophoresis system,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/Screen-Shot-2020-11-04-at-6.45.02-PM.png,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/14167E4E-043F-4D7D-AEB2-C169303F42F6.jpeg,",36.0802326000,-86.8320743000,09/24/2020,"
Was caught attached to a Red CarpenterAnt and was later detached. Was found at the base of a tree with other ants nearby.<\/p>\n",Formicidae,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/75880592-5E0B-45DB-85BB-2F7380860B8D.jpeg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/KX003-scaled.jpg,",42.4850931,-71.43284,09/26/2020,"
We used a trap to catch the organism; took place during the fall. Area was grassy but lacked other vegetation<\/p>\n",Insecta,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/Screenshot-2020-11-04-at-9.23.43-AM.png,",42.4850931000,-71.4328400000,,"",Araneae,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/02/156.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/Screenshot-2020-11-04-at-9.27.52-AM.png,",42.4850931000,-71.4328400000,,"",Isopoda,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/02/157.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/S-Rao-2-scaled.jpg,",42.4797274508,-71.4579901895,09/22/2020,"
This Strawberry Seed Beetle was caught in the woods near our school. The cup used to catch the beetle was embedded in the ground and surrounded by lots of leaves which fell from the trees. It’s unclear exactly when the beetle was caught, but the week or so the trap was set out was during autumn.<\/p>\n",Harpalus rufipes,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/02/Screen-Shot-2021-02-02-at-8.42.35-AM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/XuK-Photo-1-scaled.jpg,",42.4850931,-71.43284,09/26/2020,"
Collection took place in the fall. The area had some grass but not many other plants or trees (it was a school outdoor garden)<\/p>\n",Armadillidae,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"","",No,,,, "",42.1680796,-88.4281415,10/02/2021,"
This carpenter ant was found in the garage, and it was quick moving, yet easy to notice on the floor as it did not blend well with its surroundings when I located it.<\/p>\n",Hymenoptera,,Abdomen,MiniOne,TBE,GelGreen,"","
This is lane #146 in the given picture. There are multiple bands below the main band for the CO1 gene, so the gene may have been truncated, or there may have been nuclear genome repeats.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Microbe-Hunters0009-scaled.jpg,",40.9580848000,-112.2145571000,,"
cold, windy, rocky, found in the morning<\/p>\n",Orthoptera,DNeasy (Qiagen),Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Screenshot-2021-04-02-11.58.43-AM.png,","
we followed the recommended conditions for Wolbachia primers only. we used the standard reagents from MiniPCR.<\/p>\n",Yes,,,, "",41.7508391,-88.1535352,10/03/2021,"
I got the bug outside my front door by the light on the top.<\/p>\n",Armadillidae,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Screenshot-2021-04-08-at-12.50.43-PM.png,","
DNA extraction: I mase sure to crush the arthropod really well. I dissected the abdomen out and didn’t include the exoskeleton.<\/p>\n
Gel electrophoresis lanes for CO1arthropod PCR:<\/p>\n
1- 1 Kb ladder<\/p>\n
2- The second well is my negative DNA extraction control.<\/p>\n
3- The third well is the positive DNA extraction control<\/p>\n
4- the fourth well is positive PCR\/DNA control<\/p>\n
5-the fifth well is positive PCR control<\/p>\n
6- the sixth well is my sample<\/p>\n
Analysis: my controls worked but my sample did not have a band at all.There could have been a problem with my extraction<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/misquito-.jpg,",41.6967777000,-88.1974148000,,"",Diptera,Puregene (Qiagen),,Standard electrophoresis system,LB,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-Gel-1-CO1-9.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/85B70068-3177-4C59-842A-5BB7A79043FF-scaled.jpeg,",37.2707022000,-76.7074571000,03/28/2021,"
I found this centipede in my backyard. It was in the grass, I took a few steps and found another one.<\/p>\n",,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_male_abdomen-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_side-scaled.jpg,",39.1031182,-84.5120196,05/20/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel6a.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_side-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_male_abdomen-scaled.jpg,",39.1031182,-84.5120196,05/20/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel6a.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_female_abdomen_1-scaled.jpg,",39.1031182,-84.5120196,05/20/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel5a.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_female_abdomen_1-scaled.jpg,",39.1031182,-84.5120196,05/20/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel1a.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_male_abdomen-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_side-scaled.jpg,",39.1031182,-84.5120196,05/20/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel6a-1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_male_abdomen-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_side-scaled.jpg,",39.1031182,-84.5120196,05/20/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel6a-1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_male_abdomen-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_side-scaled.jpg,",39.1031182,-84.5120196,05/20/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel4a.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_female_abdomen_1-scaled.jpg,",39.1031182,-84.5120196,05/20/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel6a-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel8a-1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_male_abdomen-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_side-scaled.jpg,",39.2785064000,-84.4025248000,05/22/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel4a.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_male_abdomen-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_side-scaled.jpg,",39.2785064000,-84.4025248000,05/22/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel4a.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_female_abdomen_1-scaled.jpg,",39.1031182,-84.5120196,05/22/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel7a.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_side-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_male_abdomen-scaled.jpg,",39.2785064000,-84.4025248000,05/22/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel7a.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/cicada_decim_female_abdomen_1-scaled.jpg,",39.1031182,-84.5120196,05/22/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel7a.jpg,","",No,,,, "",39.1031182,-84.5120196,05/21/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel-623-2.jpg,","",No,,,, "",39.1031182,-84.5120196,05/21/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel-623-3.jpg,","",No,,,, "",39.1031182,-84.5120196,05/21/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel-623-4.jpg,","",No,,,, "",39.1031182,-84.5120196,05/21/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel-623-5.jpg,","",No,,,, "",39.1031182,-84.5120196,05/21/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel-623-6.jpg,","",No,,,, "",39.1031182,-84.5120196,05/21/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel-623-7.jpg,","",No,,,, "",39.1031182,-84.5120196,05/21/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel-623-8.jpg,","",No,,,, "",39.1031182,-84.5120196,05/21/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel-623-9.jpg,","",No,,,, "",39.1031182,-84.5120196,05/21/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel-623-10.jpg,","",No,,,, "",39.1031182,-84.5120196,05/21/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel-623-11.jpg,","",No,,,, "",39.1031182,-84.5120196,05/21/2021,"",Magicicada septendecim,Thermoscientific GeneJet Genomic Purification,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/06/Gel-623-12.jpg,","",No,,,, "",36.1444896,-86.8011462,08/28/2023,"
N\/A<\/p>\n",Culex pipiens,none,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/IMG_2655-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/PXL_20230907_174812907-1-scaled.jpg,","
JH-MQ = Mosquito<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/IMG_1548-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/IMG_1549-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/IMG_1553-scaled.jpeg,",37.806977504218516,-122.42673595455213,08/30/2023,"
Dead Moth found in the garden in a spider\u2019s web<\/p>\n",Hexapoda,none,Partial abdomen,MiniOne,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/IMG_1624-1-scaled.jpeg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/PXL_20230829_181936397-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/PXL_20230829_181936397-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/PXL_20230829_181934145-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/PXL_20230829_181916606-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/PXL_20230829_181856931-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/PXL_20230829_181836771-scaled.jpg,",36.1444896,-86.8011462,08/28/2023,"
~ Thorax: N\/A<\/p>\n
~ Abdomen: Black\/Light Brown – Stone like pattern<\/p>\n
~ 7 (Visible) Brown hairy Legs (Almost thorn-like)<\/p>\n
~ Under Dissecting Microscope ~ Legs were covering up the head<\/p>\n",Araneae,none,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"","
JH-1401 = Noble Widow Spider<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/WIN_20230828_12_56_40_Pro.jpg,",44.92657783545501,-93.17277640979049,08/28/2023,"
Around 1.5 cm length, red-brown, many legs, segmented body<\/p>\n",Oxidus gracilis,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2023/09/IMG-2538-scaled.jpg,",44.9516643000,-93.1629063000,08/28/2023,"
Black, small with white\/ greyish legs. Round lines around the outer shell.<\/p>\n",Armadillidae,,,,,,"","",,,,, "",44.9516643,-93.16290629999999,11/09/2023,"
Mulch outside, late summer 78 degrees Fahrenheit, urban environment<\/p>\n",Hymenoptera,,,,,,"","",,,,, "",44.9263520000,-93.1724445000,08/28/2023,"
Found the ant in the mulch outside the school. It was late summer, urban habitat, and was 78 degrees Fahrenheit<\/p>\n",Hymenoptera,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/09/72CA4BE1-A75D-465C-92A4-F5EBF02407A9-scaled.jpeg,",37.1224466,-76.4447397,11/03/2020,"
Has two wings and is green underneath. A little bit stripped on top. 4 legs.<\/p>\n",Diptera,Edwards Buffer,Partial abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/09/0151F045-8E9E-4580-ABBD-898DAA386823.jpeg,","
-for DNA extractions, after I put the partial abdomen into the micro centrifuge I accidentally added 150 microlitters instead of 100<\/p>\n
-for PCR steps, when making the master mix we thought we made enough for 10 (we had 9), but we had to made a little bit more because it ran out for some reason.<\/p>\n
-our PCR machine did not work and we had to push experiment back a few days. Everything was stored safely and at correct temp and we made new gel on day of experiment.<\/p>\n
-we did not need to add much green dye because our DNA was already died a couple days before. However in order to made it easier to pick up with a micro centrifuge we added 5 micro liters of green dye.<\/p>\n",Yes,,,, "",37.08634463483119,-76.47168785400389,10/15/2020,"
– very small<\/p>\n
– black body<\/p>\n
– missing two legs<\/p>\n",Hymenoptera,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/09/677F3C87-F09E-4B5A-80FC-3EC6F3371717.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/09/34BA3B28-2927-41A9-9D7A-2C6C3F34A656.jpeg,","
– used same controls throughout the gel tests<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/09/CD3D0FF4-7FD2-4185-B1C3-F152C73C627F-scaled.jpeg,",37.1195900000,-76.4586100000,09/01/2021,"
Was very docile and was a little startled after collection.<\/p>\n",Coleoptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/09/4FAA5557-CE46-491D-9A0D-DBEF0442B95A.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/09/BF7637EA-42B8-4DED-995E-27B7D5D80226.jpeg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2021/09/4E0BEB79-8258-4805-B1BC-CB53F74955E0.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/09/92DD7B15-F213-4D52-95E0-BCA7FB2193D4.jpeg,",370645.0000000000,762921.0000000000,08/08/2021,"
4 pairs of legs, 8 in total, with 2 body segments. Brown body with translucent legs and brown knobs for joints. The body is fuzzy.<\/p>\n",Araneidae,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/09/E3FB7580-64DF-4F01-80ED-6103748876EA.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/09/BF7216D6-AABD-453D-B048-86D404A9B7B4-scaled.jpeg,","
We had to redo the second arthropod gel due to a mistake made with the gel at first, and may have gone too roughly with pulling it out (which can be seen with the less visible wells in the second gel). Complications with cross-contamination may have occurred, with (more or less confident) false negatives in 01-A, 06-A and the Wolbachia-Arthropod DNA which should<\/em> all have had positive bands, however 01-A and 06-A could have been done correctly.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/09/GrasshopperP1_ssmv_wolbac-scaled.jpg,",36.1377126819,-87.0265197329,08/18/2021," post-rain, evening, 73 degrees Fahrenheit, humid<\/p>\n",Orthoptera,DNeasy (Qiagen),Partial abdomen,MiniOne,TBE,GelGreen,""," Typical standard operating procedures<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/11/tendai1-scaled.jpg,",38.9071923000,-77.0368707000,09/22/2021," brown<\/p>\n",Hemiptera,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/11/IMG_5300.jpg,"," DNA extraction: I made sure to crush the arthropod really well. I dissected the abdomen, and did not include the ext<\/p>\n <\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/09/19CA28DD-8FF6-40EC-B57E-034677B9387E.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/09/32C89E79-4C76-48D2-94BC-C8E1DF2F8688.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/09/79E0314C-1B68-4299-A8EC-E30024363E55.jpeg,",42.1185667258,-76.2554270832,09/11/2021," Small town, grassy and shaded area during the day. Came out at night, flew close to the ground and would land in the grass<\/strong><\/p>\n",Lepidoptera,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"","",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/G31-scaled.jpg,",42.4836453,-71.4418101,09/10/2021,"",Harpalus rufipes,DNeasy (Qiagen),Abdomen,MiniPCR,TAE,GelGreen,"","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/image0-3-scaled.jpeg,",40.4397476000,-74.2692229000,05/02/2021," A female anopheles mosquito<\/p>\n",Hexapoda,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TBE,Other,"","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/02/Screenshot-2022-02-10-7.24.21-AM.png,",40.4397476000,-74.2692229000,05/02/2021," Female culex mosquito<\/p>\n",Hexapoda,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TBE,Other,"","",Yes,,,,
"",1.425425,103.775333,02/23/2022,"",Formicidae,Instagene Matrix,Whole arthropod,Edvotek Gel Electrophoresis,PBS,Other,"","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/IMG_2255-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/IMG_2256-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/IMG_2258-scaled.jpg,",42.4792618000,-71.1522765000,09/14/2021,"",Steatoda triangulosa,Edwards Buffer,Partial abdomen,Edvotek Gel Electrophoresis,TBE,GelGreen,"","",Unknown,Primers given by Wolbachia Project,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/Leafhopper-Image.png,",42.4884618,-71.13296849999999,09/14/2021," Small, green, winged insect<\/p>\n",Cicadellidae,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/03/Screen-Shot-2022-03-30-at-1.46.12-PM.png,","",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/04/image0-6-rotated.jpeg,",43.5108577229,-72.1188484209,03/23/2022," Dead upon collection<\/p>\n",Insecta,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,GelRed,"","",Unknown,,,,
"",37.0909442466,-796.5309992964,04/12/2022," – hard outside \/ brown shade<\/p>\n – curled up when touched<\/p>\n – multiple legs \/ cream shade<\/p>\n",Armadillidae,Edwards Buffer,Abdomen,MiniPCR,,GelGreen,"","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Arion.jpg,",47.4618426,8.9968358,04/05/2022,"",,Instagene Matrix,Abdomen,MiniOne,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Gel4.jpg,","",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/PXL_20220506_131806914-scaled.jpg,",38.0619511099,-84.7324612017,05/02/2022," Malaise Trap<\/p>\n Malaise trap<\/p>\n",Theridiidae,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG-5149-scaled.jpg,",38.05257599999999,-84.7299464,05/02/2022," Caught in malaise trap. blue-green beetle, mandibles, six white spots,<\/p>\n",Coleoptera,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG-1575-scaled.jpg,",38.05257599999999,-84.7299464,05/16/2022," pincer butt, six legs, antenna, brown<\/p>\n",Insecta,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG-0399-scaled.jpg,",38.05257599999999,-84.7299464,05/05/2022," Netted Trap, killed with alcohol, hot\/humid day, next to a water source<\/p>\n",Insecta,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"","",No,,,,
"",38.061929,-84.7324955,05/02/2022," Malaise trap<\/p>\n",Diptera,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/Screenshot-2022-05-09-2.48.40-PM.png,",38.05257599999999,-84.7299464,05/05/2022," Netted trap, killed with alcohol, hot\/humid day, next to a water source<\/p>\n",Insecta,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG-2658-1-scaled.jpg,",38.0525760000,-84.7299464000,05/02/2022," Malaise Trap<\/p>\n",Drosophila melanogaster,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG-1577-1-scaled.jpg,",38.0630000000,-84.7310000000,05/02/2022," Green-blue color, iridescent, six-legged, 2 antennae, stomach a deeper blue than the rest of the body.<\/p>\n",Coleoptera,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG_9575-scaled.jpg,"," Processed bug DNA was combined with the dye so that Wolbachia could be seen during gel electrophoresis, and the samples were loaded into the gel. The samples were run for ~20 minutes, then photographed and analyzed.<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG-1581-scaled.jpg,",38.0630000000,-84.7310000000,05/02/2022," Wings, large eyes, somewhat iridescent, six-legs, long and thin tube-like mouth.<\/p>\n",Diptera,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG_9575-1-scaled.jpg,"," Processed bug DNA was combined with the dye so that Wolbachia could be seen during gel electrophoresis, and the samples were loaded into the gel. The samples were run for ~20 minutes, then photographed and analyzed.<\/p>\n",Yes,,,,
"",38.0630000000,-84.7310000000,05/02/2022," smll, long legs, dark, small body.<\/p>\n",Diptera,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"","",No,,,,
"",38.05257599999999,-84.7299464,05/02/2022," Black beetle shell with a dark black body. The beetle has very skinny legs with a yellow circle hanging off its body.<\/p>\n",Coleoptera,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG_0389-scaled.jpg,",38.05257599999999,-84.7299464,05/02/2022," Malaise trap<\/p>\n",Diptera,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/09/IMG_4372.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/09/IMG_4373.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/09/IMG_4371.jpg,",40.5892172000,-74.4124027000,09/20/2022," This mosquito is a female because it has similar features like other female mosquitoes.<\/p>\n",Culex,DNeasy (Qiagen),Abdomen,MiniOne,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/10/ashlynn.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/10/Rudra-1.jpg,"," Qiagen blood+tissue kit – with modification P2 for ATL + N3 for AL<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/10/IMG_7778-scaled.jpg,",40.58006547331567,-74.41139903173828,," Male\/Female specify Large, yellow, 6 long skinny yellow legs, 2 wings.<\/p>\n",Nephrotoma,DNeasy (Qiagen),,MiniOne,Other,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/11/BCE41640-8408-4BE9-9F3A-00FE94E9726E-scaled.jpeg,"," Multiple individuals inhabiting the same area<\/p>\n",Pyrrharctia isabella,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/12/P.-isabella-Arthropod-Gel.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/12/P.-isabella-Wolbachia-Gel.jpg,"," Cut caterpillar into six sections from head to posterior, performed PCR and ran gel on each duplicate.<\/p>\n",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2022/12/caterpillar_2-CO1F-1.fa,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/12/IMG_2684-rotated.jpg,",39.1151064000,-78.8258543000,10/30/2022," Multiple species feeding in the same location<\/p>\n",Estigmene acrea,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/12/E.-acrea-1-Arthropod-Gel.jpg,"," Caterpillar was cut into six slices. All six duplicates were tested for Wolbachia.<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2022/12/caterpillar_1-CO1F.fa,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG-3023.jpg,",31.9708128000,-81.1586515000,01/23/2023," The insect was collected from underneath a log. On the day of collection it was cold, windy and overcast.<\/p>\n",Coleoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Bianca-Elaina-2.png,"," DNA extraction kit of in-house reagents was used<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Earwig-drawing-color-scaled.jpg,",31.9708128,-81.15865149999999,01/23/2023," Collected at 1:10pm in front of student recreation center by the faculty parking lot.<\/p>\n Sunny 57 degrees Fahrenheit found under log with pine straw ground was wet.<\/p>\n",Dermaptera,,Partial abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Gel-Eletrophoresis.jpg,"," DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_1092-scaled.jpeg,",31.9708128,-81.15865149999999,01/23/2023," DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_8456-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_8712-scaled.jpeg,",31.9781563000,-81.1632034000,01/24/2023," Inside of tree bark. Dark and damp environment<\/p>\n",Isoptera,,Whole arthropod,,,,"","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Screen-Shot-2023-04-11-at-6.18.39-PM.png,",31.9781563000,-81.1632034000,01/24/2023,"",Neuroptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Screen-Shot-2023-04-11-at-6.24.05-PM.png,"," DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/image0-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/image1.jpeg,",31.9708128000,-81.1586515000,01/23/2023," Time – 1:00 pm – 1:30 pm<\/p>\n Location – The Armstrong Post office, Art center, Science center parking lot<\/p>\n Environment – In tree branch<\/p>\n Collected by grabbing handful of pine straw, dirt, grass, and sprinkling it all over collection tray to pick out moving creatures. Breaking branches off from trees, moving bushes.<\/p>\n",Hymenoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/image0-2.jpeg,"," DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Picture1.png,",31.9793811851,-81.1609571464,01/26/2023," Collected with bug net and beat and stick method in the morning when it was cold. Insect appears green with two pairs of wings.<\/p>\n",Neuroptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Picture2.png,"," DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,,
"",37.6806986973,-122.1396292605,04/05/2023," -On the wall<\/p>\n -It was warm outside with a cool breeze<\/p>\n -It wasn’t moving<\/p>\n -It was going crazy inside our container<\/p>\n",Tipulidae,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/20230810_135454-scaled.jpg,",45.515232,-122.6783853,08/10/2023," This insect was found on a milkweed plant in an urban garden.<\/p>\n",Chlorochroa,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Chlorochroa-gel-results.png,"," We use GoTaq Green Master Mix, 1% agarose gel, 1% TAE buffer, and 95 V for one hour.<\/p>\n",No,,,,
"",41.7886079000,-87.5987133000,08/07/2023,"",Hymenoptera,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TBE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Nguyen_EastCoastBaddies-1.jpg,"," We followed the protocol on the website.<\/p>\n Top gel is Arthropod gel.<\/p>\n Bottom gel is Wolbachia gel.<\/p>\n The sample is in lane 3 for the top gel<\/p>\n The sample is in lane five for the bottom gel<\/p>\n We might have swapped the samples.<\/p>\n <\/p>\n",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/98A66EFE-E828-414D-89A3-396EBF1FCC29.jpeg,",41.7886079,-87.5987133,08/08/2023,"",Diptera,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TBE,GelRed,""," We followed the project protocol. In our gel image, the top lanes have arthropod primers, and the bottom lanes have wolbachia primers. And my sample is in lane 2.<\/p>\n",Yes,,,,
"",41.7886079000,-87.5987133000,08/08/2023,"",Formicidae,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TBE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Nguyen_EastIes-1.png,"," We followed the protocol on the website.<\/p>\n the top gel is wolbachia<\/p>\n bottom gel is arthropod<\/p>\n The sample is in third lane of both gels<\/p>\n",Unknown,,,,
"",41.7886079000,-87.5987133000,08/07/2023,"",Isopoda,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TBE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Nguyen_EastCoastBaddies.jpg,"," We followed the protocol on the website.<\/p>\n Top gel is Arthropod gel.<\/p>\n Bottom gel is Wolbachia gel.<\/p>\n Sample is in Lane two of gel one and lane four of gel two<\/p>\n We might have swapped the samples.<\/p>\n <\/p>\n",Unknown,,,,
"",41.7886079000,-87.5987133000,08/08/2023,"",Armadillidae,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TBE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Nguyen_EastIes.png,"," We followed the protocol on the website.<\/p>\n the top gel is wolbachia<\/p>\n bottom gel is arthropod<\/p>\n The sample is in second lane of both gels<\/p>\n",No,,,,
"",41.7886079000,-87.5987133000,08/08/2023,"",Armadillidium vulgare,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TBE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Screen-Shot-2023-08-09-at-2.37.07-PM.png,"," Followed the protocol. The top lanes are arthropod primers and the bottom lanes are Wolbachia primers. The sample is in lane 2.<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Screenshot-2023-08-09-144108.png,",41.8377404000,-87.6057645000,08/07/2023," Regular Pillbug<\/p>\n",Isopoda,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TBE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Nguyen_LA-Gel.png,"," We followed the protocol on the website.<\/p>\n Top gel: arthropod DNA primer<\/p>\n Bottom gel: wolbachia DNA primer<\/p>\n sample in the first lane for both gels<\/p>\n",No,,,,
"",41.7886079,-87.5987133,08/08/2023,"",Armadillidium,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TBE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Barber_DTA-1.jpg,"," We followed the protocol on the website. The top lanes use the arthropod primer and the bottom lanes use the wolbachia primer.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Screenshot-2023-08-09-144245.png,",41.8377404000,-87.6057645000,08/07/2023," small, thin, red caterpillar found in the dirt of a garden<\/p>\n",Lepidoptera,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TBE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Nguyen_LA-Gel-1.png,"," We followed the protocol on the website.<\/p>\n Top gel: arthropod DNA primer<\/p>\n Bottom gel: wolbachia DNA primer<\/p>\n sample in the second lane for both gels<\/p>\n",Yes,,,,
"",41.7886079000,-87.5987133000,08/07/2023,"",Sphecius speciosus,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TBE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Barber_Butterfly.tif.jpg,"," We followed website protocol.<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Pillbug-Image.jpg,",41.7886079,-87.5987133,08/08/2023," Dark gray pillbug.<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TBE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Nguyen_TATT-1.png,"," We followed the protocol on the website. The top gel is the Arthopod gel and the bottom is the Wolbachia gel.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Butterfy-Image.jpg,",41.7886079,-87.5987133,08/07/2023," Black body with white wings.<\/p>\n",Pieris rapae,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TBE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Nguyen_TATT.png,"," We followed the protocol from the website. The top gel is the Arthopod gel and the bottom is the Wolbachia gel.<\/p>\n",No,,,,
"",41.7886079000,-87.5987133000,08/07/2023,"",Formicidae,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TBE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Barber_Butterfly.jpg,"," We followed the protocol on the website.<\/p>\n",Yes,,,,
"",41.7886079000,-87.5987133000,08/08/2023," The pill bug was small with a hard outer shell. The insect’s color was a light grayish-brown. It was difficult to sacrifice.<\/p>\n",Armadillidae,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Screen-Shot-2023-08-09-at-2.39.06-PM.png,"," We followed the protocol on the website<\/p>\n",Yes,,,,
"",41.7886079,-87.5987133,08/08/2023," Very small spider<\/p>\n",Insecta,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TBE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/08/Barber_DTA.jpg,"," We followed protocol on website.<\/p>\n Followed the Wolbachia website protocol.<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/06/Screen-Shot-2023-06-08-at-8.51.40-AM.png,",40.6200478000,-74.5682307000,06/07/2023," We found the bug sitting on the edge of a log. The bug was very well camouflaged and looked exactly like a living leaf. While dissecting the bug, we saw a blue-like substance inside the abdomen which we couldn’t identify.<\/p>\n",Hemiptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/06/Screen-Shot-2023-06-12-at-3.40.14-PM.png,"," The sample extracted from the Pale Green Assassin Bug is in lane 4. The top half of the gel shows results for the CO1 gene, and the bottom half shows results for the 16S gene. We prepped the samples and then ran them through a gel for approximately 20 minutes at 100v. We then ran the gel for approximately 15 minutes at 10v while waiting to view results. The sample in Lane 4 (Pale Green Assassin Bug) had a band that lined up with the band from the + arthropod control, meaning our putative insect had a positive band at the CO1 arthropod gene. The sample in lane 4 (Pale Green Assassin Bug) also had a band that lined with the expected + Wolbachia control band, meaning the insect had the 16S gene and Wolbachia infection.<\/p>\n",Yes,TGGATCACCTCCCCCAGATGGGTCAAAGAATGAAGTATTAAAGTTTCGATCAGTTAATAGCATAGTAATAGCCCCTGCTAAAACAGGAAGAGATAATAATAAAAGTAATGCAGTAATTCCTACTGATCAGACAAATAATGGGATTCGTTCAGGAGTTATTCCAGCAGGTCGTATATTAATAATTGTTGAAATAAAATTAACTGCTCCTAAGATTGAAGATACTCCGGCTAAGTGTAATGAGAAAATTGCTAAATCTACAGCTGCACCTCTATGAGCTATATTACTAGATAAGGGGGGATAAACAGTCCATCCTGTTCCTGCCCCTCCTTCAGCAATTCTTCTAATTAATAACAAGGTTAGGGAAGGGGGAAGAAGTCAAAATCTTATATTATTTATTCGGGGGAAGGCTATATCAGGAGCTCCAATTATAAGGGGTACTAATCAATTCCCAAATCCTCCAATTATAATAGGCATAACTATAAAGAAAATCATAATAAATGCGTGTGCAGTAACGAATACATTATAAATTTGATCATTTCCAATAAAAGTACCTGGAGTACCTAATTCAATTCGAATTATTCATCTTAAGGAGGTTCCAATAACCCCTGCTCAAGCACCTAGCAGAAAATATAGAGTTCCAATATCTAT,https://wolbachiaprojectdb.org/wp-content/uploads/2023/06/MD01-Premixed-2.fa,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/Screen-Shot-2023-05-30-at-12.56.19-PM.png,",40.6214813733,-74.5676666797,05/30/2023," Specimen collected from underneath a log. The specimen is an even dark brown and has two antennae, 7 pairs of legs, and two uropods; it possesses essential characteristics of the common woodlouse, commonly referred to as a sowbug.<\/p>\n",Crustacea,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/06/Screen-Shot-2023-06-05-at-9.31.21-AM-1.png,"," 10-fold dilutions (20\u00b5l\u2192<\/b> 18\u00b5l of distilled water, 2\u00b5l of original sample) were performed on template PCR due to the original DNA concentrations of the sample being too high, causing no reading on the gel. The gel submitted is the gel taken of the diluted samples, the second gel overall.<\/p>\n",Unknown,GCTGTTGGAACAGCCCTTAGGATAATTATTCGAACTGAGTTAGGAAACCCTGGGAGGTTAATTGGAGACGATCAGATTTATAATGTGATTGTTACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGCTTTGGTAATTGATTAGTACCTTTAATATTAGGGGCCCCTGATATAGCTTTTCCACGTATAAATAATATAAGATTTTGGCTTCTTCCCCCATCACTAATTTTGTTATTAAGAAGAGGTTTAGTTGAAAGAGGTGTTGGGACTGGCTGGACTGTCTACCCACCTTTGGCAAGTGGAATTGCCCATAGAGGGGCTTCAGTAGATTTAGGGATTTTTTCCCTTCATTTGGCTGGAGCTTCTTCAATTTTAGGAGCTGTAAATTTTATTACTACTGTAATTAATATACGAGCAGAGGGGATAAGGATAGATCGTGTCCCTCTATTTGTTTGGTCAGTGTTAATTACAGCAATTCTCTTGTTACTTTCACTACCAGTTTTATCAGGGGCCATTACTATGTTATTAACAGACCGTAATCTGAACACCTCTTTTTTCGACCCTAGGGGGGGAGGAGATCCTATCTTGTACCAACACTTATTTTGATTTTTTGGTCACACAAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2023/06/MD18-Premixed-1.fa,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/06/IMG_2469-1.jpg,",40.6202235000,-74.5677355000,06/05/2023," Black, about the size of 2-3 rice grains<\/p>\n Three distinct parts- abdomen, thorax, head<\/p>\n Glossy abdomen shell with linear stripes across it<\/p>\n <\/p>\n",Pterostichus permundus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/06/Screenshot-2023-06-08-at-2.23.52-PM.png,"," Our gel consisted of two rows. Row 1 contained DNA samples with Wolbachia primers and Row 2 contained samples with Arthropod Primers. We ran our gel for about 35 minutes. There are disparities within the size of the gel wells because when making the gels we used two different combs. See the gel image for classification of wells. When analyzing the gel, bands slightly beneath 1000bp signify the presence of\u00a0 Arthropodal DNA(708 bp is the location at which the Arthropod DNA is found). Bands slightly beneath 500bp signify the presence of Wolbachia DNA(438bp is the location at which the Wolbachia DNA is found).<\/p>\n In Row 1 our controls proved successful. Bands beneath 500bp were shown for the positive control but not the negative control(columns 4 and 5). However, no bands were exhibited beneath 500bp in columns 2 and 3(the columns that contained the DNA of our collected Arthropods), meaning that Wolbachia was not amplified in our actual PCR samples.<\/p>\n In row 2 our controls proved successful again. Bands beneath 1000bp were shown for both controls(columns 4 and 5). Both controls were Arthropods so this was expected. In column 3, a band was exhibited beneath 1000bp, confirming that Anthropodal DNA was amplified in my PCR. However, it was hard to confirm the presence of a band in column 2 because DNA ladder bled into column 2 due to a rupture in the wall barrier between the first and second wells.<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2023/06/MD15-Premixed-1.fa,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/IMG_6866.jpg,",40.6202235000,-74.5677355000,05/30/2023," The Leucauge venusta <\/em>spider is distinctly colored with leaf-green legs. The abdomen is shiny capsule-shaped and tubular. Yellow markings appear on the abdomen and black blotches are on the end of it. There is a straight black line with smaller lines extending from the midline down the center of the abdomen. The legs are green with black bands at the joints. On the rear of the abdomen, there is a small red spot.<\/p>\n",Leucauge venusta,DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/06/Screen-Shot-2023-06-08-at-9.09.15-AM.jpeg,","",Yes,TTTTNNGTGCTTGAGCTTCATAATAGGGACAGCTATAAGTGTGCTAATTCCGATTGAGTTACGTCAA TCGGGAAGATTTTTAGGCAATGATCAATTGTATAATGTGATTGTAACAGCTCATGCTTTTATTATAATTTTTTTTATAAT TATACCTATTTTAATTGGGGGATTTGGAAATTGATTGGTTCCTTTAATATTAGGGGCTCCTGATATGGCTTTTCCTCGCA TAAATAATTTAAGTTTCTGACTATTACCTCCTTCACTCTTTTTATTATTTATTTCTTCAATGGTAGATATANGAGTGGGG GCTGGGTGAACTGTATATCCTCCTTTAGCTTCTTTAAAAGGGCATTCTGGTAATTCTATGGATTTTGCTATTTTTTCTCT TCATTTAGCTGGGGCTTCTTCTATTATAGGGGCTATTAATTTTATTTCTACTATTTTTAATATACGGGTAAATGGAATAA AAATATAAAAATTTCCTTTATTTGTTTGATCTGTGTTAATTACNNNTGTTTTATTATTATTATCCCTCCCGGTTTNGGCN NGGGCNANTACTATATTGNTAACANATCGCANTTTTAATACTTCTTTTTNTGACCCTTCTGGANGANGGGATCCAGTTCT TTTTCCACATTNATTTTNATTTTTNGGNNNNNNNANNTTAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2023/06/MD16-Premixed-2.fa,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/F3B9A94B-F998-42D3-9F5B-BE25C4504ED0_1_105_c.jpeg,",40.6224804817,-74.5774208498,05/30/2023," Found spider sharing a web, which had been woven between the branch and main body of a fallen tree, with another spider of a different species (Neriene radiata<\/em>). This spider is green with a silver, black, and yellow abdomen. Its cephalothorax had appeared slightly translucent.<\/p>\n",Leucauge venusta,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/06/Screen-Shot-2023-06-08-at-9.14.21-AM.png,"," We accidentally used ladder instead of loading dye when we were preparing our samples, and as a result, our gel was a little hard to read. However, the ladder solution had loading dye in it, so the DNA still ran through the gel. We re-ran the whole process, but our new gel ended up having no results, not even in our controls, so we kept this gel.<\/p>\n The sample extracted from the Leucauge venusta<\/em> is in lane 2 (shown in the table). We prepped the samples and then ran them through a gel for about 15 minutes at 100 watts. The top half of the gel shows results for the C01 arthropod gene, and the bottom half shows results for the 16s Wolbachia gene. The sample in lane 2 had a band aligned with the band from the positive arthropod control, showing a positive result for the CO1 arthropod gene for the spider. The sample in lane 2 also had a band that lined up with the positive Wolbachia control, meaning the spider had the 16S Wolbachia gene in addition to the arthropod CO1 gene.<\/p>\n Reddish orange wings with black spots. White M shape on head with small black spots. Rounded oval like dome, two antennas and 6 small legs. Black inner body covered by wings.<\/p>\n",Coleoptera,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/06/IMG_0254.jpg,"," 1% Agarose gel set at 140 for approximately 30 minutes.<\/p>\n",Unknown,GGAATAGTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAGAGGAAGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTTTGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTTTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGATAAAACTCCTTTATTTGTATGATCTGTTCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTATTAACTGACCGAAACTTAAATTCTTCTTTTTTTGACCCAACCGGTGGGGGAGACCCAATTTTATACCAACAGTTATT,https://wolbachiaprojectdb.org/wp-content/uploads/2023/06/MD05-Premixed-1.fa,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/06/IMG_6D18741E4543-1.jpeg,",40.6202235000,-74.5677355000,06/02/2023,"",Hymenoptera,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/06/image_6487327.jpg,"," Wolbachia Positive control is lane 2<\/p>\n The ladder is lane 1<\/p>\n The Wolbachia sample is lane 7<\/p>\n",Yes,GGCTCCTCTATAAGAATAATCATTCGACTAGAGTTAGGATCTCCTGATTCACTAATTCTTAATGATCAAA CTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGG GGGATTTGGTAATTTTTTAATTCCACTTATACTAGGATCTCCTGATATAGCTTACCCTCGTTTAAATAAC ATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTG GAACTGGTTGAACTATCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGAC TATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACAATTATA AATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTAGTATGATCTATTCTTATTACAGCTA TTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGCGCTATTACAATACTACTAACAGACCGAAATCTTAA TACTTCATTTTTCGATCCCTCGGGAGGAGGAGATCCTATTTTATACCAACATTTATTTTGATTTTTTGG,https://wolbachiaprojectdb.org/wp-content/uploads/2023/06/CSMD14-Premixed.fa,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/06/image_67111937-scaled.jpg,",40.6202235,-74.5677355,05/30/2023," The beetle has a smooth matte black outer shell and its legs have hooks on the end. The beetle is approximately 2.5cm long and 1cm wide. We found the beetle in healthy conditions, but upon closer inspection under a dissection microscope, it looks as if the shell on the thorax may have been split into two halves after we caught the bug.<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/06/Screen-Shot-2023-06-08-at-9.13.35-AM.png,"," The sample extracted from the False Mealworm Beetle is in lane 4. The top half of the gel shows results for the CO1 gene, and the bottom half shows results for the 16S gene. We prepped the samples and then ran them through a gel for approximately 15 minutes at 100v. We then used a UV light to view results. The sample in Lane 4 (False Mealworm Beetle) had a band that lined up with the band from the + arthropod control, meaning our putative insect had a positive band at the CO1 arthropod gene. The sample in lane 4 (False Mealworm Beetle) did not have a band at expected + Wolbachia control band, so we feel the beetle was negative for Wolbachia infection.<\/p>\n",No,CCGAGCAGAGCTCGGCAATCCTGGCTCCCTTATTGGAGACGATCAAATCTACAACGTAATCGTAACCGCCCATGCATTTATCAGAATTTTCTTCATAGTTATACCAATCATAATTGGAGGTTTGGGAAATTGATTAATACCCCGAATGCTAGGAGCTCCTGACATAGCATTCCCACGAAAAAATAGCATAATATTTTGACTACTTCCCCCATCACTAACATTGTTACTAATAAGAAAAATTGGGGAAAGAGGGGCCAGAACAGGATGAACAGGTTATCCCCCCCTATCATCAAACTTTGCCCATGGTGGATCATCTGTAGATTTAGCAATTTTTACCCAACCCTTAGCAAGAATCTCTTCTATTCGAGGTGCTGTGAATTTTATTACTACAGTAATTAATATACGGCCCCAAGGAATAAGATTTGATCGAATACCNCTATTTGTATGATCAGTAATAATTACGGCCGGACTATTACTACTATCTTTACCAATACTANCANGA,https://wolbachiaprojectdb.org/wp-content/uploads/2023/06/MD06-Premixed.fa,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/97F954A1-EE07-4641-979F-AE1EE1C84C6B.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/DABDAA95-4B91-47AE-B0F5-30525C774782.jpeg,",34.0937481000,-118.0211796000,04/13/2023,"",Chelicerata,DNeasy (Qiagen),body without wings,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/0D12DD97-3A73-43E9-8665-5C5BCDCBF569.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/5DB659EC-7E26-4A56-B97B-A30EA15C6BFB.jpeg,","",No,The Chelicerata was found to be negative for Wolbachia.,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/15341983-E54C-4DA4-88A0-5018FF95017A.jpeg,",34.0937481000,-118.0211796000,06/25/2023,"",Insecta,DNeasy (Qiagen),body without wings,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/00964996-27FF-48FE-A43A-3E7D2041BEC2.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/5180D503-6ADD-45B3-A264-C874A4E884F1.jpeg,","",No,The insects was found to be negative for Wolbachia.,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/spider-biotech--scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/spider--scaled.jpeg,",34.0937481,-118.0211796,04/05/2023," It was on a wall in a shady area, when you looked from far away the spider looked like it only had four legs. It was dark brown in color and moved very quickly as we appraoched it.<\/p>\n",Parasteatoda tepidariorum,none,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/wolbachia-data-1-1-scaled.jpeg,"," We ran a regular PCR protcol twice but both times the data was faint and even our control was off. So our data was unreliable.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/Subject-4.png,",37.6729393000,-122.1348629000,04/06/2023," -Looks like a normal spider<\/p>\n -Around 5 mm in length<\/p>\n",Parasteatoda tepidariorum,DNeasy (Qiagen),,Standard electrophoresis system,LB,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/0CFCB74A-A8E9-4945-9AA6-2C9D25A4EB61-scaled.jpg,","",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/IMG_2658.jpg,",37.6816992261,-122.1393307710,04/07/2023," Prenolepis imparis 4mm length<\/p>\n Metasoma ~1.8-1.9 mm<\/p>\n Head ~.8-.9mm<\/p>\n 4 visible legs (some legs possibly lost during transportation)<\/p>\n small mesosoma ~1 mm<\/p>\n 2 long antennas<\/p>\n Dark brown color<\/p>\n 3 white stripes on the metasoma<\/p>\n <\/p>\n <\/p>\n <\/p>\n",Insecta,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/IMG_5729-scaled.jpeg,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/pincher-bug--scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/picher-bug-2nd--scaled.jpeg,",34.0937481,-118.0211796,04/05/2023," We found the pincher roaming around and used tweezers and a cup to capture it. In order to preserve the the spider we place it into alcohol. It was wandering in a shady area, it was dark brown in color and was very still as we approached it.<\/p>\n",Forficula auricularia,none,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/wolbachia-data--scaled.jpeg,"," We ran a pcr, using the body of the insect to collect as much DNA as possible. We had a negative control and a postive control. Lane 3 is where we tested the pincher for wolbachia but unfortunately something happened and none of the wells showed up. Even if any fragments did show up, out tube’s labels were erased so out data is inconclusive.<\/p>\n",Unknown,,,,
"",37.6811238664,-122.1404065192,04/06/2023," Dark red orange elytra with symmetrical black spots, black striped abdomen, six legs intact, wings intact<\/p>\n",Coccinellidae,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/IMG-1193-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/IMG-1200-scaled.jpg,"," We made a 1% agarose gel for this protocol, with a positive arthropod control, negative arthropod control, and a positive DNA control. We also tested our samples for Arthropod CO1 to verify that we actually got arthropod DNA.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_1091-scaled.jpg,",34.0937481000,-118.0211796000,04/06/2023," Tan color, long legs, large wings, hairy legs, long thin abdomen<\/p>\n",Tipulidae,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/IMG-1193-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/IMG-1200-scaled.jpg,"," We made a 1% agarose gel for this protocol, with a positive arthropod control, negative arthropod control, and a positive DNA control. We also tested our samples for Arthropod CO1 to verify that we actually got arthropod DNA.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_0819-scaled.jpeg,",37.6811953000,-122.1406461000,04/06/2023," found in grassy well lit area. flying around in a group with other crane flies<\/p>\n",Tipulidae,qiagen blood and tissue,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/IMG_0955-scaled.jpeg,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/IMG_6998-scaled.jpeg,",37.6810294250,-122.1405696000,04/07/2023," -Under a piece of wood<\/p>\n -Found near spiders and rollypolly<\/p>\n -Damp dirt<\/p>\n",Forficula auricularia,DNeasy (Qiagen),Rear half,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/IMG_7050-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/IMG_7055-scaled.jpeg,"," Obtained by lifting a piece of wood in a damp, undisturbed area, picking up the arthropod, and placing it in a container.<\/p>\n",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/IMG_3202-scaled.jpg,",37.6805137821,-122.1419131068,04/06/2023," -Damp location<\/p>\n -Ground was Dirt<\/p>\n -Found next to a slug<\/p>\n -Found under a rock<\/p>\n",Chilopoda,DNeasy (Qiagen),Rear half,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/IMG_7055-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/IMG_7050-1-scaled.jpeg,"," We obtained the centipede by lifting a rock in an unbothered area that happened to hide the insect and simply retrieved it into a container.<\/p>\n",Unknown,,,,
"",37.68048125545585,-122.13873263141137,07/01/2023," Ladybug<\/p>\n",Coleoptera,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/IMG_5736-scaled.jpg,",37.6803099945,-122.1426626038,04/06/2023," Had dark brown spots on translucent yellow tan legs, dark brown abdomen<\/p>\n",Arachnida,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/arthropod-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/wolbachia.jpg,"," The spider is hairy and had a big stomach. We found the spider dead in the bricks. It also had dirt all over the legs.<\/p>\n",Araneae,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/IMG_7636-scaled.jpg,","",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/mosquito-scaled.jpg,",37.6804681000,-122.1386400000,04/06/2023," The Cucline Mosquito had a slim body, long legs and shorter wings. The wings had a yellowish color and the body was a dark brown with black accents. The mosquito had a 2.2cm body. The arms and legs were hairy.<\/p>\n",Insecta,DNeasy (Qiagen),Abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/mosquitogel-1-e1683908683943.jpg,","",No,,,,
"",37.6804745093,-122.1385901172,04/05/2023," 2 cm in length, clear yellow legs, orange antennas with dark black eyes, dark brown abdomen, sharp brown pinchers.<\/p>\n",Crustacea,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,Other,""," <\/p><\/blockquote>\n",Unknown,,,,
"",37.6809327201,-122.1407447017,04/06/2023,"",Chilopoda,DNeasy (Qiagen),Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/6EED59CC-D426-4E27-AE84-1A5AEE5AF29C-scaled.jpeg,","",No,,,,
"",37.6804615000,-122.1386374000,04/06/2023," Small in size (1in. tall), abdomen and limbs hairy, spinneret not visible, abdomen black and cephalothorax is caramel brown. Produced a visibly “tangled” cobweb at the end where the spinneret would be (a clump of cobweb we assumed was just dust.)<\/p>\n",Steatoda,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/4C7AEF58-C914-416E-8221-39FF07FA8B59.jpeg,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/304959221_1553334971787984_4357659140411610621_n.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_1046-scaled.jpeg,",43.3274286300,-111.0990943000,09/05/2022," Stonefly Nymph- Under rock in the river<\/p>\n Description: Brown with a red hue<\/p>\n Body length: 1.27 cm. Found by Danny Ahlstrom<\/p>\n",Drunella coloradensis,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,""," Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod.<\/p>\n",No,GGACTTCGTTGAGGTTACGCATCCAGGCAGAACTAGGGCAACCTGGGTCAGTNNTGGGANATGACCGNNCNANAATGGCATTGTAACGNCCATGCCTTCATTATAATTTTCTTTATGGTAATACCTATTATAATTGGGGGATTCGGGAATTGATTAGTTCCGTTAATGCTTGGTGCCCCCGATATAGCATTTCCCCGAATGAACAATATAAGCTTTTGGTTGTTACCTCCTGCCCTAACGTTATTATTGGCTAGAAGTATAGTGGAAAGAGGGGCAGGAACCGGTTGGACAGTTTACCCCCCCTTGGCTTCCGGCATTGCCCATGCTGGGGGATCTGTAGATCTTGCTATCTTTTCGCTTCACTTAGCGGGAGTCTCCTCTATTCTCGGAGCAGTAAATTTTATTACAACCACCATTAATATACGAGCCAGAGGAATAACTATGGACCGAATTCCCCTATTTGTATGGTCCGTTTTAATTACCGCAATCCTCCTTCTTCTCTCCTTACCGGTCCTTGCTGGGGCTATTACTATGCTTCTTACAGACCGTAACTTAAATACCTCGTTCTTTGACCCGGCGGGTGGGGGAGACCCTATTTTATACCAACATCT,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/304936242_837393520593637_2134841359397667949_n.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_1001-scaled.jpeg,",43.3281135510,-111.0984294000,09/05/2022," Beetle Grub- Under a log in brush along dirt path<\/p>\n Description: tan and yellow color<\/p>\n Body length: 2.03 cm. Found by Charyn Brooks<\/span><\/p>\n",Prosternon bombycinum,Edwards Buffer,Other,Standard electrophoresis system,TAE,,""," Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod.<\/p>\n",No,GATATTAGGAACTTCATTAAGATTATTAATTCGGGCAGAACTAGGAAATCCAGGATCATTAATCGGAAATGACCAAATTTACAACGTTATTGTCACAGCACATGCTTTCATTATAATTTTCTTCATAGTTATACCAATCATAATTGGGGGATTCGGAAATTGATTAGTACCCCTAATACTAGGAGCACCTGATATGGCATTCCCACGAATAAATAACATAAGATTCTGATTTCTACCCCCATCACTATCTCTTCTATTAATAAGAAGAATTGTAGAAAATGGAGCAGGAACAGGCTGAACAGTTTATCCCCCCCTCTCAGCAAACATTGCCCATAGTGGATCTTCAGTTGATTTAGCCATTTTCAGTCTACATTTAGCAGGAATCTCATCAATCTTAGGAGCAGTAAATTTCATTTCAACTGTTATTAATATACGAACAACAGGAATCACATTCGACCGAATACCTTTATTTGTATGAGCCGTAGCAATTACAGCTCTTCTTCTATTATTATCTTTACCTGTATTAGCAGGAGCCATCACAATATTACTTACTGACCGAAATCTAAATACATCATTTTTTGATCCTGCAGGAGGAGGAGATCCAATCCTATACCAACACCTATTCT,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/305049973_2273054729524747_5681879847166357273_n.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_0813-scaled.jpeg,",43.3273906550,-111.1013268000,09/05/2022," Woodland Butterfly- Long blades of green and yellow grass in open area<\/p>\n Description: Tan with orange markings<\/p>\n Body length: 1.78 cm. Found by Charyn Brooks<\/p>\n",Ochlodes sylvanoides,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,""," Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod.<\/p>\n",No,ATTTGAGCAGGAATATTAGGAACTTCTTTAAGTTTATTAATTCGTACAGAATTAGGTAATCCAGGATCTTTAATTGGCGATGACCAAATTTATAATACTATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGATTAGTTCCATTAATATTAGGAGCTCCTGATATAGCATTTCCTCGAATAAATAATATAAGATTTTGAATATTACCTCCTTCATTAACATTATTAATTTCAAGAAGAATTGTAGAAAATGGAGCAGGAACTGGTTGAACAGTATATCCTCCTTTATCTTCTAATATTGCTCACCAAGGATCTTCTGTTGATTTAGCAATTTTTTCTCTTCATTTAGCTGGTATTTCATCTATTCTAGGAGCTATTAATTTTATTACAACAATTATCAATATACGAATTAAAAACTTATCATTTGATCAAATACCCTTATTTGTATGATCAGTAGGTATTACAGCATTATTATTATTATTATCTTTACCTGTATTAGCAGGTGCTATTACAATATTACTTACTGATCGAAATTTAAATACTTCTTTTTTTGATCCAGCAGGAGGAGGAGATCCAATTTTATATCAACATTT,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/304972785_2119169568248994_8527417502731279268_n.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_0806-scaled.jpeg,",43.3269011850,-111.1049029000,09/05/2022," Stink Beetle- underneath large rock in brush moist ground<\/p>\n Description: Big shiny black large thorax.<\/p>\n Body Length: 2.28 cm. Found by Chase Hartzell<\/p>\n",Carabus taedatus,Edwards Buffer,Partial abdomen,Standard electrophoresis system,TAE,GelGreen,"","",No,TACTTCTCTAAGAATACTAATTCNAGCTGAATTGGGAAATCCCGGATCCTTAATTGGAGATGATCAAATTTATAATGTTATTGTAACAGCCCATGCTTTCGTAATAATTTTTTTCATAGTTATACCTATTATAATTGGGGGATTTGGAAATTGATTAGTGCCACTAATATTAGGAGCACCAGATATAGCCTTCCCTCGAATAAATAACATGAGATTTTGACTTTTACCCCCTTCCTTGACCCTTCTCCTAATAAGTAGAATAGTTGAAAAAGGGGCAGGGACAGGTTGAACAGTTTACCCTCCGCTGTCATCAGGAATCGCTCACAGAGGGGCATCAGTGGACCTAGCTATTTTTAGACTTCATTTAGCAGGAATTTCATCAATTTTAGGAGCAGTAAATTTTATTACAACAATTATTAATATACGATCAGTAGGGATAACATTTGATCGAATACCCTTATTTGTATGATCAGTAGGAATTACAGCATTATTATTATTACTATCTTTACCAGTTTTAGCTGGAGCTATTACTATACTTCTAACAGATCGAAAATTTAAATACTTCTTTTTTTGATCCTGCCGGAGGGGGAGATCCTATTCTTTATCAA,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/11/305176892_771275094140270_7703659294187995228_n.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2022/11/IMG_0802-scaled.jpeg,",43.3268920000,-111.1054330000,09/05/2022," Caught on human leg flying by the dry dirt pathway in the hot sun.<\/p>\n Description: Dark coloring, transparent wings, lighter abdomen than thorax.<\/p>\n Body length: 1.2 cm.<\/p>\n Found by Lola Ahlstrom<\/p>\n",Peleteria iterans,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/11/Screenshot-2022-11-10-at-1.42.45-PM.png,","",No,AGGAACATCTTTAAGAATTTTAGTGCGAGCTGAATTAGGACATCCAGGTGCATTAATTGGAGATGATCAAATTTATAATGTAATTGTTACAGCTCATGCATTTGTAATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGATTTGGAAATTGATTAGTACCATTAATATTAGGAGCCCCTGATATAGCTTTCCCTCGAATAAATAATATAAGTTTTTGACTTTTACCACCTGCATTAACTTTATTACTTGTAAGAAGAATAGTGGAAAATGGAGCTGGAACAGGATGAACTGTTTACCCTCCATTATCATCTAATATTGCACATGGAGGAGCTTCTGTAGACTTAGCAATTTTTTCTTTACATTTAGCAGGAATTTCTTCTATTCTAGGAGCTGTAAATTTTATTACAACTGTTATTAATATACGATCTGTAGGAATTACTTTTGATCGAATACCTTTATTTGTTTGATCAGTAGTTATTACAGCATTATTATTACTTTTATCATTACCAGTATTAGCTGGAGCTATTACTATATTATTAACAGATCGAAATTTAAATACTTCATTTTTTGATCCTGCAGGAGGAGGAGATCCTATTTTATATCAACATTTATTTTG,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/305019081_449299743912221_4377165125393298065_n.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/305085175_375536181456475_3948325426607778230_n.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_0775-scaled.jpeg,",43.3269162890,-111.1080235000,09/05/2022," Carpenter Ant\/found under nesting soil. Found under a rock surrounded by dry dirt and leaves. Many ants were found at the time, and we selected one out of the group.<\/p>\n Description: Black head and abdomen with a light-colored yellow orange thorax.<\/p>\n Body length: 1.2 cm. Found by Zander Brooks.<\/p>\n",Camponotus vicinus,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"","",No,TATAAGAATAATTATTCCCTTAGAACTAGGATCCACTAACTCACTAATCATAAATGATCAAACTTTTAATACTATTGTTACAAGACATGCTTTTATCATAATCTTTTTTATAGTTATGCCCTTCATAATTGGGGGATTTGGTAACTTCTTACTCCCCCTAATACTCGGATCTCCTGATATAGCCTATCCCCGTATAAACAACATAAGATTTTGATTACTCCCCCCATCAATCTCCCTCCTAATTCTAAGAAATTTTATTAATGAGGGATCTGGAACAGGTTGAACTATTTATCCCCCCTTAGCGTCTAATACCTTCCATAGGGGTTCATCTATTGATCTAACCATCTTCTCCCTTCNTATTGCTGGTATGTCATCAATTCTTGGAGCANTTAATTTTATTTCAACAATTATAAATATACATAATACTAATATCTCATTAGATAAAATTCCCCTCTTANTTTGATCTATTCTTATCACAGCAATCCTTCTCCTCCTATCCCTCCCCGTCCTTGCCGGANCTATTACNATATTATTAACAGATCGAAATCNTAATACTTCCTTCTTCGATCCATCTGGTGG,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Screenshot-2023-04-27-10.35.35-AM.png,",37.6804710000,-122.1386433000,04/07/2023," Can jump and is very agile.<\/p>\n",Salticidae,DNeasy (Qiagen),Abdomen,,Other,,"","",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/78EC8107-AD65-4FB9-A419-4536A6D6A435-scaled.jpeg,",37.6810704205,-122.1402534247,04/06/2023," At a wooden picnic table on grassy land, underneath a tree. Found between the wooden planks of the table.<\/p>\n 8 legs, orange\/brown hue, pinchers observed, 0.1cm in length, light line vertically across abdomen, smooth body<\/p>\n",Pseudoscorpiones,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/05/AA73F130-607B-4483-A3A7-95D275F6E441.jpeg,","",No,,,,
"",41.1920872,-81.5110772,04/04/2023," Samll, fast, lived amongst leafy areas<\/p>\n",Hexapoda,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/This-one-2-e1682616145708.jpg,"," All for Flying Ant<\/p>\n",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/20230404_133848-1-scaled.jpg,",41.1920872000,-81.5110772000,04/04/2023," Fast, small, lived amongst leafy areas.<\/p>\n",Hexapoda,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/This-one-2-e1682616145708.jpg,","",No,,,,
"",41.23601578762862,-81.53545568702773,04/04/2023," Pavement ant with wings. A few mm in length.<\/p>\n",Hexapoda,DNeasy (Qiagen) blood and tissue kit,,Standard electrophoresis system,TAE,GelGreen,"","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_20230411_133215977_2.jpg,",41.234805426968805,-81.5419196790451,04/04/2023," Black beetle with long antennae. Around 1.5 cm in length.<\/p>\n",Hexapoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,GelGreen,"","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_20230411_132854711_2.jpg,",41.1740315000,-81.5212410000,03/31/2023," Black midge. About 1 cm in length.<\/p>\n",Hexapoda,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_0384-scaled.jpg,",31.9708128000,-81.1586515000,01/25/2023," A DNA extraction kit of in-house reagents was used.<\/p>\n",No,TGAGCAGGTATAATTGGAACATCCCTAAGAATTCTAATTCGAGCAGAATtacgttgacCCGGTTCCTTAATTGGTAATGATCAAATTTATAatcttatTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCTATTATAATTGGCGGATTTGGTAACTGACTAGTCCCATTAATAATTGGAGCCCCTGATATAGCATTCCCTCGAATAAATAATATAAGATTTTGACTTTTACCACCCTCTTTATTATTATTATTATTAAGAAGAATAGTAGAAAATGGAGTTGGTACCGGATGAACAGTTTATCCCCCTTTATCATCCAATATTGCTCACGGAGGTTCATCAGTTGATTTAGCTATTTTTAGTTTACACTTGGCTGGAATTTCTTCAATTTTAGGAGCAATTAATTTTATTACTACAGTAATTAATATACGTCCAATAGGAATAACTTTTGACCGAATACCATTATTTGTATGAGCAGTTGTTATTACAGCAGTTTTACTACTATTATCATTACCTGTTCTTGCCGGAGCAATTACAATATTATTAACAGATCGTAATCTAAATACCTCTTTTTTTGATCCTGCTGGAGGTGGTGATCCTATTTTATATCAACATTTATTTTGATTTTTTG,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_0965-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/insect-2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/insect.jpg,",31.9708128,-81.15865149999999,01/25/2023," Date of collection<\/b>3<\/b>: <\/b>1\/25\/2023<\/p>\n Time of collection<\/b>3<\/b>: <\/b>1:12 PM<\/p>\n Weather conditions<\/b>3<\/b>: <\/b>Cool, gloomy day- right before rain<\/p>\n Location of collection<\/b>3<\/b>: <\/b>Outside of Science Center and Greenhouse on Georgia Southern Armstrong Campus<\/p>\n Environment of collection (habitats<\/b>)5:<\/b> Bushes, Trees, dirt, under tree branches on the ground, open area of grass<\/p>\n Method of collection<\/b>5<\/b>: <\/b>3 methods of collection<\/p>\n DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_6871.jpg,",,,01/25/2023," Caught in bushes and trees with an arial net then by hand.<\/p>\n",Hymenoptera,,body without wings,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_6872.jpg,"," DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_0848-scaled.jpg,",31.9794037194,-81.1610258362,01/25/2023," Time of collection: 1:21pm<\/p>\n Location: in front of Jenkins Hall in the shrubs<\/p>\n Environment of Collection: Gloomy about 72 degrees<\/p>\n Method of collection: Sweeping Net<\/p>\n",Hymenoptera,,Abdomen,Standard electrophoresis system,TAE,Other,""," DNA extraction kit of in-house reagents was used<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Picture1-3.png,",,,01/25/2023," A DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,TATTTGGAGCATGAGCAGgaataGTAGGAACATCTCTAAGAATTTTAATTCGAGCTGAATtacgt ttcCCAGGAGCTTTAATTGGAGATGATCAAATTTATAAccttattGTTACTGCACATGCTTTTGT AATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGATTTGGAAATTGACTAGTCCCATTAA TATTAGGAGCACCTGATATAGCATTTCCTCGAATAAATAATATAAGTTTTTGATTACTTCCACCA TCATTAACACTTTTAATGGCTAGAAGTATAGTGGAAAATGGAGCCGGAACAGGATGAACTGTATA TCCACCTTTATCTTCAATTATTGCACATGGGGGAGCTTCAGTAGATTTAGCTATTTTTTCTCTTC ATTTAGCTGGGGTATCATCAATTTTAGGAGCAGTAAATTTTATTACTACAGTAATTAATATACGA TCTACAGGAATTACATTCGATCGAATACCATTATTTGTATGATCAGTAGTAATTACTGCTTTATT ATTACTATTATCACTACCAGTATTAGCAGGAGCCATTACTATATTATTAACTGATCGAAATTTAA ATACTTCATTCTTTGATCCTGCAGGAGGAGGGGATCCAATTTTATATCAACATTTATTTTGATTT TTTGGTCA,,,
"",31.9781563,-81.1632034,01/25/2023,"",Orthoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_0403-scaled.jpg,"," DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG-7188-scaled.jpg,",,,01/25/2023," Time ~ The insects were collected within ten minutes between Location ~ Armstrong campus by the parking lot between the Natural Environment ~<\/p>\n They were found below a big tree and bushes in the Warm and gloomy day and clear environment<\/p>\n 71 degrees<\/p>\n Method of Collection ~ The insects were first collected by the A DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG-4577-scaled.jpg,",31.9708128,-81.15865149999999,01/25/2023," Location: Tea Olive Tree outside science center<\/p>\n Time and Date: 1409 1\/25\/2023<\/p>\n Weather: 71<\/p>\n Method: Beating<\/p>\n",Hexapoda,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_0407-scaled.jpg,"," DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_9755-scaled.jpg,",31.9708128,-81.15865149999999,01/25/2023," DNA extraction kit of in-house reagents was used<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_1222.jpeg,",31.9708128000,-81.1586515000,01/24/2023," Caught on branches of trees located on the Georgia Southern University – Armstrong Campus. Collected around 530PM using the beat tray and stick. Weather was roughly between 50-55 degrees Fahrenheit at time of collection.<\/p>\n",Psocodea,none,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/0-1.jpeg,"," DNA extraction kit of in-house reagents was used<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/69708745349__BB228CCE-A983-450D-9E3E-114BB92F7016.jpg,",,,01/24/2023," Savannah, Ga, ponds at GSU Armstrong Campus. Used a D-net, scooping method. Sunny, dry, and cold. Took many tries to find anything.<\/p>\n",Odonata,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Samuel_Gabby-and-Jenna.png,"," DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_3940-scaled.jpg,",31.9708128,-81.15865149999999,01/25/2023," Location: The Pond (North)<\/p>\n Date: 1\/25\/2023<\/p>\n Time: Afternoon 1:10 pm<\/p>\n Weather: Warm\/Cloudy\/50 degree<\/p>\n Habitat: Sand Area Near The Pond<\/p>\n",Hymenoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/DSCN3176_.jpg,"," DNA extraction kit of in-house reagents was used<\/p>\n",No,CGTGCTGATCCACGATTACTAGCGATTcctatttcATGCACTCGAGTTGC AGAGTACAATCCGAACTGAGATGGCTTTTAAGGGATTAGCTTAGCCTCGC GACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTC CATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAgttTATC ACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCTGATGGTAACTAAGGAT GAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCT GACGACAGCCATGCAACACCTGTGTGAAATCCGGCCGAACCGACCCTATC CCTT,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Hymenoptera-Pic.jpg,",,,01/26/2023," Location: The Pond(North) DNA extraction kit of in-house reagents was used<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/4C2F9026-705D-4E7E-B022-CCC18EC0AAB5-scaled.jpeg,",31.9806798699,-81.1581400735,01/26/2023," Found near a large point under a fallen, rotting tree branch that was slightly damp.<\/p>\n",Isoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/98F3BC3D-6D49-42FB-BCEA-9C4B7678493E.jpeg,"," DNA extraction kit of in-house reagents was used<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_8502-scaled.jpg,",31.9708128000,-81.1586515000,," 26 Jan 23<\/p>\n 10:00am<\/p>\n bush with pink flowers<\/p>\n Georgia Southern Campus<\/p>\n Stick and sheet<\/p>\n",Hemiptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Picture1-1.png,"," DNA extraction Kit of in house reagent was used<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Blattodea.png,",31.9708128,-81.15865149999999,01/23/2023," Date of Collection- Thursday 1\/26\/23<\/span><\/p>\n Time of Collection- 10:12 AM<\/span><\/p>\n Location of Collection- Northeast side of pond of the Georgia Southern Armstrong campus<\/span><\/p>\n Address: 11935 Abercorn St, Savannah, GA 31419<\/span><\/p>\n Environment (habitat) of Collection- Windy, Cold temp. (49<\/span>0<\/span>F), Edge of muddy bank with algae floating in the shaded water<\/span><\/p>\n Methods of Collection-\u00a0<\/span><\/p>\n <\/p>\n",Blattodea,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/gel.jpg,"," DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_B9A33A5D28F6-1.jpeg,",31.9708128,-81.15865149999999,01/26/2023," Collection was taken on the east side of science center. The collection method was with tweezers and by hand. We found specimen under logs. The weather was overcast, windy and cold.<\/p>\n",Hymenoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Diago-Briana-Peyton-1-scaled.jpg,"," DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Screen-Shot-2023-04-13-at-9.35.37-AM.png,",31.9708128,-81.15865149999999,01/26/2023," DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_8618-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_8662-scaled.jpeg,",31.9708128000,-81.1586515000,,"",Diptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Diago-Briana-Peyton-2-scaled.jpg,"," DNA extraction kit of in-house reagents was used<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG-7520-scaled.jpg,",31.9810004916,-81.1593149939,01/26/2023," DNA extraction kit of in-house reagents was used<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Screenshot_20230405_122505_Gallery.jpg,",31.9708128000,-81.1586515000,01/10/2023," Collected 10 January, 2023 at 10 A.M.<\/p>\n Collected with Beat Stick and Canvas<\/p>\n Environment: Flower bed:Azalea Bush<\/p>\n <\/p>\n <\/p>\n",Hemiptera,,Rear half,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Josilynn-Patrice-1-scaled.jpg,"," DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/american-cockroach-dorsal.jpg,",32.0808989,-81.091203,01/26/2023," DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Coleoptera-Drawing.jpg,",31.979663117389805,-81.1611066140568,01/25/2023," DNA extraction kit of in-house reagents was used<\/p>\n",No,GTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAAttacgatctAgagGAAGATTAATTGGAAA CGACCAAATTTATAAtactattGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATAC CTATTATAATTGGGGGTTTTGGAAATTGAtTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCA TTTCCACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTTTAATTTTAAG AACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTTTACCCTCCTCTTTCTTCTAATTTAA CACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATT TTAGGTGCAGTAAATTTCATttcaaCTATTATAAATATACGTCCATTTGGT,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_0844-1-scaled.jpg,",40.16618067815734,-76.60713221940887,10/12/2022," female mosquito<\/p>\n <\/p>\n female mosquito<\/p>\n <\/p>\n <\/p>\n <\/p>\n female mosquito<\/p>\n <\/p>\n <\/p>\n <\/p>\n female mosquito<\/p>\n",Aedes albopictus,Wizard SV (Promega),Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG_0849-scaled.jpg,"," <\/p>\n <\/p>\n female mosquito<\/p>\n <\/p>\n <\/p>\n <\/p>\n female mosquito<\/p>\n <\/p>\n <\/p>\n <\/p>\n female mosquito<\/p>\n <\/p>\n <\/p>\n<\/p>\n",Chauliognathus lugubris,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG-0592.jpg,",38.0600000000,-84.7310000000,05/02/2022,"
<\/p>\n",Formicidae,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG_20220506_091016648-scaled.jpg,",38.05257599999999,-84.7299464,05/02/2022,"
Small beetles with orange head and abdomen. Black wings and two black stripes on head behind eyes.<\/p>\n",Coleoptera,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG_1490-scaled.jpeg,",37.987723,-84.7315563,05/02/2022,"
Malaise trap<\/p>\n",Insecta,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG_1491-scaled.jpeg,",38.05257599999999,-84.7299464,05/02/2022,"
Malaise trap<\/p>\n",Insecta,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/2DB583CC-C136-40C3-9852-DC3E1738C5A7-1-1.jpeg,",38.05257599999999,-84.7299464,05/02/2022,"
malaise trap<\/p>\n",Dermaptera,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"","",No,,,,
"",38.05257599999999,-84.7299464,05/06/2022,"",Ceratopogonidae,Instagene Matrix,Whole arthropod,Standard electrophoresis system,PBS,Fast Blast,"","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/05/IMG-1580-scaled.jpg,",38.05257599999999,-84.7299464,05/16/2022,"
\nCollected by the<\/p>\n",Culex,Monarch DNA extraction (NEB),Abdomen,MiniOne,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2022/10/emma-2.jpg,","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/11/99A77F43-C2C1-42A6-9C25-32A27F3EE721-scaled.jpeg,",38.9071568176,-77.0374500571,09/18/2022,"<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2022/12/IMG_2685-rotated.jpg,",39.1151064000,-78.8258543000,10/09/2022,"
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\n1:20 PM.<\/p>\n
\nlibrary and Student Union. Jenkins Hall<\/p>\n
\nground within soil and leaves.<\/p>\n
\nsweeping method with the aerial net, but then it became more
\neffective to use my hands to dig in the soil.<\/p>\n",Dermaptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/IMG-7621.jpg,","
\nDate:01\/26\/23
\nTime: Morning 10:00 am
\nWeather: Sunny\/Windy\/Cold 48 degrees
\nHabitat: Wet Area Near Edge Of Pond<\/p>\n",Hymenoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Gel-Electrophoresis-scaled.jpg,","\n
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\n 1\/25\/23<\/td>\n<\/tr>\n \n Ladybugs<\/td>\n<\/tr>\n \n 2:09pm<\/td>\n<\/tr>\n \n Cloudy and wet<\/td>\n<\/tr>\n \n In front of Science Center<\/td>\n<\/tr>\n \n On the tea olive tree<\/td>\n<\/tr>\n \n Beating net<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n",Coleoptera,,Whole arthropod,,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2023/04/Screenshot-2023-04-12-at-2.46.03-PM.png,","