# This file is a CSV export of the The Wolbachia Project database on Apr 15 2021 05:43:31 GMT
image_urls,location_lat,location_lon,collection_date,observations,putative_identification,dna_extraction_kit,dna_extraction_location,gel_rig,running_buffer,dna_stain,gel_image_urls,protocol_notes,wolbachia_presence,wolbachia_16s_sequence,wolbachia_16s_fasta,wolbachia_16s_ab1,arthropod_COI_sequence,arthropod_COI_fasta,arthropod_COI_ab1,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Screenshot-2021-04-09-at-7.51.52-AM.png,",41.6967777000,-88.1974148000,,"<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Found in dirt and mulch<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">\u00a0near plants<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">\u00a0tried to dig itself underground\u00a0<\/span><\/li>\n<\/ul>\n",Hymenoptera,Puregene (Qiagen),,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-Gel-2-Wspec-7.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-Gel-1-CO1-6.png,","",No,TTTTTAGTATATTATATATTCATCTGGCTTTTTGGATGCACCGATGAGAGATCCAGTTTTCACAGCGAACGCTATGGCTT,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/55-CO1-F.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/bug-pic-scaled.jpg,",,,10/01/2020,"<p>The insect was trailing up and down the stone in a single file line with the other insects of its species, carrying things.<\/p>\n",Tetramorium immigrans,,,,,,"","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/57-CO1-F.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/IMG_0695-scaled.jpg,",41.7080786000,-88.1924259000,10/05/2020,"<p>I found the boxelder bug on my brick wall house. I found it early in the morning like 6 or 7 . The bug was very tame went into the bag easily.<\/p>\n",Rhopalidae,Puregene (Qiagen),Whole arthropod,Standard electrophoresis system,LB,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/BOXELDER-bug-.png,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/41-CO1-F.seq_.fasta,,
"",41.6854670000,-88.1985810000,10/04/2020,"<p><span style=\"font-weight: 400;\">The grasshopper was just hopping around with the other grasshoppers there and vibing in the grass.<\/span><\/p>\n",Orthoptera,Puregene (Qiagen),,Standard electrophoresis system,LB,GelRed,"","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/65-CO1-F.fasta,,
"",,,,"",,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/IMG_1893-scaled.jpg,",41.6928217000,-88.1947075000,10/04/2020,"<p>ant was walking around on the cement with no other ants. there were some leaves and twigs around the location of the ant as well<\/p>\n",Formicidae,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-Gel-2-Wspec-9.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-Gel-1-CO1-8.png,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Screenshot-2021-04-09-at-7.57.15-AM.png,",41.6967777000,-88.1974148000,10/04/2020,"<p>I found my insect walking on the sidewalk. I covered it with the cup right away, and it immediately moved to the edge of the cup, as it was trying to get out. It was very active, and even when I put it in the bag, it was still moving around a lot.<\/p>\n",Nysius raphanus,Puregene (Qiagen),,Standard electrophoresis system,LB,GelRed,"","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/63-CO1-F.fasta,,
"",,,,"<p>Stuck on a web<\/p>\n",Chilopoda,DNeasy (Qiagen),Whole arthropod,,,,"","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Screenshot-2021-04-09-at-7.55.53-AM.png,",41.7508391000,-88.1535352000,10/04/2020,"<p><span style=\"font-weight: 400;\">The bug didn\u2019t move a lot especially when I put it in a cup and tried to transfer it to the Ziploc bag. It was damp outside today because during the night it rained and so the plant that the bug was resting at was pretty moist. The plant was also a few feet in the air and hangs so it isn\u2019t for an insect to get there.<\/span><\/p>\n",Hermetia illucens,Puregene (Qiagen),,Standard electrophoresis system,LB,GelRed,"","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/68-CO1-F.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Screenshot-2021-04-09-at-7.53.12-AM.png,",41.6809839000,-88.1437782000,10/04/2021,"<ul>\n<li aria-level=\"1\">It was collected in the morning &amp; the outside temperature was 60 degrees Fahrenheit<\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Spider was located in a cobweb in the top corner of the garage\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Spider is smaller than a grain of rice<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Light brown in color<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Legs are not long, look proportional to body\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Slightly bulbous abdomen\u00a0<\/span><\/li>\n<\/ul>\n",Theridiidae,Puregene (Qiagen),,Standard electrophoresis system,LB,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-Gel-1-CO1-4.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-Gel-2-Wspec-5.png,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/58-CO1-F.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Screenshot-2021-04-09-at-7.56.20-AM-2.png,",40.4000000000,88.1000000000,10/22/2026,"<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">It went from my patio chair to under my patio table<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">It flew in short distances<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">It is a brown\/black bug with transparent wings<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">It was moving around a lot and was more by man made structures<\/span><\/li>\n<\/ul>\n",Chironomidae,Puregene (Qiagen),,Standard electrophoresis system,LB,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-Gel-1-CO1-7.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-Gel-2-Wspec-8.png,","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/fruit-fly.png,",41.7306000000,-88.1976700000,10/04/2020,"<p><span style=\"font-weight: 400;\">The fly was flying at a rather fast pace and was very anxious it seemed. I had to trap it with a banana since it was so fast. As it is incredibly small, it is hard to distinguish many features without a magnifying glass.<\/span><\/p>\n",Drosophila melanogaster,Puregene (Qiagen),Whole arthropod,Standard electrophoresis system,LB,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/wolbachia-data.png,","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/dobsonfly.jpg,",41.0000000000,88.0000000000,10/05/2020,"<ul>\n<li>lived in shady grass<\/li>\n<li>possibly flying<\/li>\n<li>midday<\/li>\n<\/ul>\n",Corydalidae,Puregene (Qiagen),,Standard electrophoresis system,LB,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-Gel-1-CO1-1.png,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Microbe-Hunters0006-scaled.jpg,",49.0124440000,-95.2396220000,03/23/2021,"<p><b><i>from a distance it looked, small, shinny, and smooth, it was black and had a hard exoskeleton\u00a0<\/i><\/b><\/p>\n<p><i><b>When I took a look up close he was a lot cooler, the legs were kind of curled, and his back had\u00a0these grayish white spots that I did not spot before, there are these little hare like things on each of its legs he looks kind of like a spider but black, round, and has less body parts<\/b><\/i><\/p>\n",Coleoptera,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Screenshot-2021-04-02-11.53.28-AM.png,","<p>we followed recommended contentions for wolbachia primer&#8217;s we only used the standerd reagent from miniCPR<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Microbe-Hunters0019-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Microbe-Hunters0018-2-scaled.jpg,",40.9580848000,-112.2145571000,03/23/2021,"",Lithobiomorpha,DNeasy (Qiagen),Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Screenshot-2021-04-02-11.48.32-AM.png,","<p>we used recommended conditions for Wolbachia Primers only. we used standard reagents for MiniPCR.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Doppelschwanz.jpg,",47.4607021502,9.0177295821,03/11/2021,"<p>place: under a piece of wood in the forest near Wil SG and Sirnach (Switzerland)<\/p>\n<p>temperature: about 10\u00b0C<\/p>\n",Campodeidae,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Gel-6-scaled.jpg,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/pseudoskorpion.jpg,",47.46034282868582,9.016856388739996,03/11/2021,"<p>place: under leaves, in the earth<\/p>\n<p>temperature: about 10\u00b0C<\/p>\n<p>weather: cloudy<\/p>\n",Cheliferoidea,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Gel-5-scaled.jpg,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Formica-rufa.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Formica-rufa-2.jpg,",47.460158852529126,9.018796180303452,03/11/2021,"<p>I found the ant under some dry leaves in a small forest.<\/p>\n",Hymenoptera,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Gel-3.jpg,","<p>Analysis: The control and SZ2 were the only one which worked.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Ohrwurm.jpg,",47.4605813707,9.0167437444,03/11/2021,"<p>Place: It was found under a piece of wood in the forest near Wil SG and Sirnach (Switzerland).<\/p>\n<p>Temperature: about 10\u00b0C<\/p>\n<p>Weather: cloudy, no rain, no sunshine, no frosting<\/p>\n<p>&nbsp;<\/p>\n",Forficuloidea,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Gel-4-scaled.jpg,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Ameise-Seite-scaled.jpg,",47.4597364670,9.0202699915,03/11/2021,"<p><img class=\"alignnone size-medium wp-image-2086\" src=\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2021\/04\/Ameise-1-225x300.jpg\" alt=\"\" width=\"225\" height=\"300\" \/><\/p>\n",Lasius niger,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Gel.png,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Schnurfusser.jpg,",47.4596196173,9.0204147590,03/18/2021,"",Unciger foetidus,Edwards Buffer,Partial abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Gel-Elektrophorese.png,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Spinne-Seite-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Spinne-oben.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Spinne-unten.jpg,",47.4594443990,9.0204043686,03/11/2021,"<p><img class=\"alignnone size-medium wp-image-2076\" src=\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2021\/04\/Spinne-300x163.jpg\" alt=\"\" width=\"300\" height=\"163\" \/><\/p>\n",Pardosa lugubris,Edwards Buffer,Partial abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Gel.png,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Gemeine-Stubenfliege.jpg,",47.4612431409,9.0166212515,03/11/2021,"",Hexapoda,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Gel.jpg,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Marienkafer.jpg,",47.4596232928,9.0203556330,03/18/2021,"",Cryptocephalus,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Gel-Elektrophorese.png,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Ameise.jpg,",47.4610098434,9.0163117342,03/11/2021,"",Lasius niger,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Gel-2.jpg,","",No,,,,
"",41.1200000000,111.9900000000,03/23/2021,"<p><span class=\"JsGRdQ\">The Stone Dwelling centipede has a more circular head that has a number of simple eyes. These are darkly pigmented and when they are numerous, they form a dark patch on each side of their head. Their antennae are quite long, usually at least one-third of the length of the body. Predator to small insects and other smaller arthropods, prey to shrews, toads, badgers, and birds.<\/span><\/p>\n",Lithobiomorpha,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"","<p>We followed the recommended conditions for Wolbachia primers only. We used the standard reagents from MiniPCR.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Screenshot-2021-04-02-12.06.31-PM.png,",41.0524417000,-112.2393920000,03/23/2021,"<p>I had went to antelope island and the weather was a lil windy and cold I had found my bug on a rock<\/p>\n",Chilopoda,DNeasy (Qiagen),Larva,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Screenshot-2021-04-02-11.55.20-AM.png,","<p>we followed the recommended conditions for wolbachia primers only we used the standard reagent from miniPCR<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Microbe-Hunters0030-1.jpg,",40.95808479999999,-112.2145571,03/22/2021,"<p>I found it in the soil under a rock<\/p>\n",Agelenidae,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Screenshot-2021-04-02-11.54.42-AM.png,","<p>we followed the recommended conditions for wolbachia primers only. we used the standard reagents from MinniPCR<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Microbe-Hunters0016-scaled.jpg,",41.0349373000,-112.2497377000,03/23/2021,"<p>This specimen was quite small for it&#8217;s species, ( about half an inch long ) leading me to believe that it was quite young.<\/p>\n",Dysderidae,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Canyon-_-Abi-3_31_21.jpeg,","<p>Followed recommended procedure for wolbachia only primers<\/p>\n",No,,,,
"",,,10/11/2020,"<p>This insect was caught in a field with tall grass.<\/p>\n",Diptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"","<p>The DNA showed a clear test and the controls worked well.<\/p>\n",,,,,
"",,,10/11/2020,"<p>This specimen was collected in a field with tall grass.<\/p>\n",Diptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"","<p>The DNA showed a clear test and the controls worked well.<\/p>\n",,,,,
"",,,10/11/2020,"<p>It was caught in a large field with tall grass.<\/p>\n",Diptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"","<p>This was conducted by professionals in a lab.<\/p>\n",No,,,,
"",,,10/11/2020,"<p>Caught in a field with long tall grass. The abdomen of the creature had a long blue fuzz growing on it. Estimated length to be half a centimeter. The pale blue fuzz disappeared when specimen was placed in the collection tube with alcohol. The fuzz has not been identified.<\/p>\n",Diptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"","<p>This was conducted in a lab setting.<\/p>\n",No,,,,
"",,,,"",,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_192042966_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_205254821_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_205251530_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_192048797_iOS-1-scaled.jpg,",36.02028991587827,-86.7378387086951,10/04/2020,"<p>on a spider plant leaf in a NE facing flower bed in a suburban back yard, 73 degrees F<\/p>\n<p>some sort of caterpillar; ~1cm long, yellow head, green underbelly, black mottled back<\/p>\n",Lepidoptera,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/Unknown-caterpillar.jpg,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_191952876_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_192009888_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_192015770_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_192002357_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_204528191_iOS-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_204946216_iOS-scaled.jpg,",36.0203210068,-86.7378564911,10/04/2020,"<p>found on spider plant leaf in NE corner of garden in flowerbed<\/p>\n<p>looks similar to a mealy bug covered in white fuzz with dark brown scaly protrusion that is bilaterally symmetrical; rest is white fluff; ~1cm<\/p>\n",Insecta,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/mealy-bug-larvae.jpg,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_191307784_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_191307001_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_204326225_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_204312595_iOS-1-scaled.jpg,",36.02028531985114,-86.73779822979762,10/04/2020,"<p>found on aster in a flowerbed, NE location in backyard behind suburban home<\/p>\n<p>2mm long, all black, two wings, delicate legs<\/p>\n",Diptera,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/unknown-black-fly.jpg,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_191114754_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_204124185_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_204101939_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_191122115_iOS-scaled.jpg,",36.02028588012979,-86.73785166558416,10/04/2020,"<p>caught in suburban back yard on an impatient flower; caught at 2:15 p.m.; 73 degrees F<\/p>\n<p>&nbsp;<\/p>\n<p>~1cm long, looks like a fly with black legs and thorax, and a yellow abdomen, two wings, large brown eyes<\/p>\n",Diptera,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/unknown-yellow-fly.jpg,","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201003_222204476_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201003_222207025_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201003_222210174_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_203547613_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_203518060_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_203536432_iOS-scaled.jpg,",36.02014505854512,-86.73802686315759,10/03/2020,"<p>some sort of spider sitting in its web which hangs from birch branch, southfacing side of the tree<\/p>\n<p>&nbsp;<\/p>\n<p>small (~1\/2cm), pointed abdomen is brown, dark thorax, pale legs<\/p>\n",Araneae,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/unknown-spider.jpg,","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201003_221655923_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201003_221650422_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_203229586_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201003_221653821_iOS-scaled.jpg,",36.02033001661619,-86.73786439621657,10/03/2020,"<p>found on stem of vine in a patch of mulch under a birch tree; found in front yard on west side of house; 5:25pm; 70 degrees F; they are aphids<\/p>\n",Coleoptera,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/aphid.jpg,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201003_221734293_iOS-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201003_221727175_iOS.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201003_221730491_iOS.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_203026899_iOS-scaled.jpg,",36.0202454969,-86.7378319642,10/03/2020,"<p>1\/2cm, ant, reddish brown color<\/p>\n<p>found on the bark of a birch tree in a suburban front yard on the west side of house; 5:20pm<\/p>\n",Hymenoptera,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/unknown-ant.jpg,","<p>did not have ladder<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201003_221121209_iOS-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201003_221103247_iOS-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_202859465_iOS-3-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_202724878_iOS-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/20201004_202752971_iOS-3-scaled.jpg,",36.0202163975,-86.7380877842,10/03/2020,"<p>found in suburban front yard on a blade of grass beneath a Bradford pear tree; fluttered around low to the ground<\/p>\n<p>~1.5cm, looks like a small moth, two antennae, two mouth parts, cream-colored<\/p>\n",Lepidoptera,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/unknown-moth.jpg,","",Yes,,,,
"",,,10/11/2020,"<p>This insect was caught in a field with tall grass.<\/p>\n",Hemiptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"","<p>This was conducted in a lab. The controls worked as expected. The bands were clear.<\/p>\n",No,,,,
"",,,10/11/2020,"<p>This insect was collected in a field with tall grass.<\/p>\n",Orthoptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"","<p>This extraction was performed in a professional lab.<\/p>\n",Yes,,,,
"",42.00014444371294,-88.35933343827395,10/11/2020,"<p>This insect was collected on a tall grass field.<\/p>\n",Diptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"","<p>This was conducted in a lab.<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/IMG_5347-scaled.jpeg,",37.0870821000,-76.4730122000,10/13/2020,"",Coleoptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-20.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-20.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-Control-2-2.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-controls-2-2.png,","<p>Visible pellet<\/p>\n<p>&nbsp;<\/p>\n",No,,,,
"",37.0870821000,-76.4730122000,10/17/2020,"",Blattodea,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/Screen-Shot-2021-03-09-at-12.57.27-PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-15.png,","<p>Small visible pellet<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/IMG_5352-scaled.jpeg,",37.0870821000,-76.4730122000,10/12/2020,"",Lithobiomorpha,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-19.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-19.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-Control-2-3.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-controls-2-3.png,","<p>No Visible pellet<\/p>\n<p>&nbsp;<\/p>\n",No,,,,
"",37.0870821000,-76.4730122000,10/16/2020,"",Hemiptera,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/Screen-Shot-2021-03-09-at-12.52.17-PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/Screen-Shot-2021-03-09-at-12.51.56-PM-1.png,","<p>Visible pellet<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/IMG_5341-scaled.jpeg,",37.0870821000,-76.4730122000,10/13/2020,"",Isopoda,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-17.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-17.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-Control-2-2.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-controls-2-2.png,","<p>Visible pellet<\/p>\n<p>&nbsp;<\/p>\n",Yes,,,,
"",37.0870821000,-76.4730122000,10/16/2020,"",Hemiptera,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-13.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-13.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-controls-1-3.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-Controls-1-3.png,","<p>Very small pellet<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/IMG_5345-scaled.jpeg,",37.0870821000,-76.4730122000,10/11/2020,"",Dermaptera,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/B941C44F-1A0A-4942-8E0B-F823E6784FB7.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/3B722820-7584-4232-ABA1-73DE707D9315.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-Control-2.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-controls-2.png,","<p>No visible pellet<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/IMG_5350-scaled.jpeg,",37.0870821000,-76.4730122000,10/12/2020,"",Blattodea,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-16.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/820AB9D4-5164-4C39-B406-C04263680B05.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-Control-2-2.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-controls-2-2.png,","<p>Visible pellet<\/p>\n<p>&nbsp;<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/IMG_5173-scaled.jpeg,",37.0870821000,-76.4730122000,10/16/2020,"<p>Visible pellet<\/p>\n",Phasmatidae,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-12.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-12.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-controls-1-3.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-Controls-1-3.png,","<p>Arthropod gel: Controls didn&#8217;t work. Water should not have a band.<\/p>\n<p>Wolbachia gel: <span style=\"font-weight: 400;\">Wolbachia DNA and controls didn\u2019t work.<\/span><\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/IMG_5344-scaled.jpeg,",37.0870821000,-76.4730122000,10/11/2020,"",Lepidoptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/937051F9-0EB2-4592-9A7A-79568C6AFB09.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/BD66C42F-DAAF-4A17-875D-874EAA275AB3.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-Control-2-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-controls-2-1.png,","<p>visible pellet<\/p>\n<p>&nbsp;<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/IMG_5171-scaled.jpeg,",,,10/16/2020,"",Orthoptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/7740F845-3D02-4312-A3F7-03708EC2605F.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/2BEEA8D4-E776-4327-945A-2FBF95FAEEE8.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-controls-1-2.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-Controls-1-2.png,","<p>Visible pellet, broken up<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/IMG_5170-scaled.jpeg,",,,10/16/2020,"",Diptera,Edwards Buffer,Whole arthropod,MiniOne,TBE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/3B3D1301-90CD-45A7-998C-9AC0EEE5A7FB.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/7C127EC9-5D63-425A-BC60-CEBDC093078F.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-controls-1-2.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-Controls-1-2.png,","<p>DNA extraction very small<\/p>\n<p>&nbsp;<\/p>\n",No,,,,
"",,,10/16/2020,"",Diptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-6.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-6.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-controls-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-Controls-1.png,","<p>Pellet visible broken up<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/IMG_5172-scaled.jpeg,",36.1444896000,-86.8011462000,10/17/2020,"<p>Visible pellet<\/p>\n",Orthoptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/874FDA5C-A8C6-4F01-861D-DD7EAAC75FCE.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/CE554412-D347-4AA9-AE8E-2EBF8356D455.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-controls-1-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-Controls-1-1.png,","<p>Arthropod gel &#8211; controls didn&#8217;t work<\/p>\n<p>Wolbachia gel- Controls and Wolbachia DNA didn&#8217;t work<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/IMG_5166-scaled.jpeg,",,,10/16/2020,"",Diptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-5.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-5.png,","<p>pellet visible, broken up<\/p>\n<p>&nbsp;<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/IMG_5169-scaled.jpeg,",,,10/16/2020,"",Diptera,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/D3E3317C-E379-487B-B6CD-14790D4C000A.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/C69C31F4-D902-4E9D-8D07-672B3635736C.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-controls-1-2.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-Controls-1-2.png,","<p>Visible pellet, broken up.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/IMG_5165-scaled.jpeg,",,,10/16/2020,"",Hymenoptera,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-4.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-4.png,","<p>visible pellet<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/IMG_5164-scaled.jpeg,",37.0870821000,-76.4730122000,10/16/2021,"",Hymenoptera,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/7C317477-E935-4E4F-BB3F-D05B407E0808.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/869A5269-F234-4299-854A-5BB2FB496A26.jpeg,","<p>DNA extraction &#8211; good, visible pellet<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/IMG_5167-scaled.jpeg,",,,10/16/2020,"",Diptera,Edwards Buffer,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/1395DB84-520A-42B7-A3FE-75FFB1CA242D.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/03/A8AD786C-17D9-4334-A7EE-FDFFE7BB42D5.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-controls-1-2.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-Controls-1-2.png,","<p>DNA extraction very small pellet.<\/p>\n<p>&nbsp;<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/IMG_5163-scaled.jpeg,",,,10/20/2020,"",Araneae,Edwards Buffer,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Arthropod-2.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/04/Wolbachia-2.png,","<p>Visible Pellet<\/p>\n<p>&nbsp;<\/p>\n",No,,,,
"",38.0002121000,-98.7445075000,08/17/2020,"<p>The box elder bugs were found on a tree and seemed to be apart of a colony. The bugs estimated infection rate is 12.5%.<\/p>\n",Hemiptera,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/02/Untitled-presentation-2.jpg,","<p>The boxelder bugs were stored in 90% ethanol for about six months prior to extraction.<\/p>\n<p>DNA Extraction: The DNA was extracted from the abdomen. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.<\/p>\n<p>PCR: Mini One Taq polymerase was used.<\/p>\n<p>Gel Electrophoresis: Mini PCR was used. All of the lanes worked well and were bright and clear.<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/compressor.png,",42.5065305495,-71.4126194567,10/01/2020,"<p>This was found in a pitfall trap baited with honey at the edge of the woods.<\/p>\n",Formicidae,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/02/Screen-Shot-2021-02-02-at-10.27.40-AM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/02/Screen-Shot-2021-02-02-at-10.27.57-AM.png,","",No,NNNNNNNNNNNNNNGNNNNNNNNNTNCTNNNNNTNNATCCTGNAANTCCNGAGNNNNGANTGGNNNNCTACCNTATNNNNNNNNNNNNNNNNNNNNNNTTANN,,,
"",38.0015316151,-98.7432222016,07/20/2020,"<p>These fireflies were collected after dark in the grass.<\/p>\n",Lampyridae,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/01/Untitled-presentation-1.jpg,","<p>The fireflies were stored in 90% ethanol for about six months prior to extraction.<\/p>\n<p>DNA Extraction: The DNA was extracted from the abdomen. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.<\/p>\n<p>PCR: Mini One Taq polymerase was used.<\/p>\n<p>Gel Electrophoresis: Mini PCR was used. All of the lanes worked well and were bright and clear.<\/p>\n",No,,,,
"",38.0012506544,-98.7440012919,08/13/2020,"<p>The bagworms were found in their cases on cedar trees.<\/p>\n",Lepidoptera,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/01/Untitled-presentation-1.jpg,","<p>The bagworms were removed from their cases and were stored in 90% ethanol for about five months prior to extraction.<\/p>\n<p>DNA Extraction: The DNA was extracted from the abdomen. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.<\/p>\n<p>PCR: Mini One Taq polymerase was used.<\/p>\n<p>Gel Electrophoresis:\u00a0 Mini PCR was used. All of the lanes worked well and were bright and clear.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2021/01/IMG_20201105_112927347-scaled.jpg,",38.9366492,-77.0402873,11/02/2020,"<p>Collected on misty morning from car door frame<\/p>\n",Tipula,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/01/Will-Damian-Gel.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2021/01/COI-and-Wolbachia-separate-gel.jpg,","<table>\n<tbody>\n<tr class=\"alt\">\n<td>We first ran duplex PCR reaction (with both COI and\u00a0<em>Wolbachia<\/em>\u00a0primers) and then ran separate PCR reactions with just COI and just\u00a0<em>Wolbachia<\/em>\u00a0primers<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\"><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2021/01/crane-fly.fa,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG_1067-scaled.jpeg,",36.1771060000,-86.7471210000,09/24/2020,"<p>The ant was easy to capture considering my hands are huge and it&#8217;s tiny. At first it tried to run away but I had sugar on my gloves and it seemed to be attracted to it. The ant was small, less then an inch small and had a thin black coat as the shell of the body.<\/p>\n",Insecta,,Abdomen,,Other,,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/12/Slide1-e1608529434513.jpg,","<p>-Wolbachia positive<\/p>\n<p>-Easy to see in gel image.<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/12/IMG_20201105_112836752.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/12/IMG_20201105_112800535-scaled.jpg,",38.9565958,-77.2094826,10/27/2020,"<p>I found this ladybug larvae on the front porch of my home underneath a light. It was the only one nearby.<\/p>\n",Harmonia axyridis,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/12/Will-Damian-Gel.jpg,","",No,CTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGATAAAACTCCTTTATTTGTATGATCTGTTCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTATTAACTGACCGAAACTTAAATTCTTCTTTTTT,https://wolbachiaprojectdb.org/wp-content/uploads/2020/12/Will_Bug_FASTA.fa,,
"",38.9382603990,-77.1027213316,11/01/2020,"<p>Found on the garage door near my house at around 3:30 PM. It had rained the night before.<\/p>\n",Aedes albopictus,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/12/COI-and-Wolbachia-separate-gel-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/12/Luke-Dr.-O-Gel.jpg,","<p>We first ran a duplex reaction. Then, we ran two separate PCR reactions: one with just Wolbachia primers, and one with just COI primers.<\/p>\n",Yes,CTAAGAGTTTTAATTCGTATTGAACTTAGACATCCTGGTATATTTATTGGAAATGATCAAATTTATAATGTAATTGTTACTGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCTATCATAATTGGAGGATTTGGAAACTGACTAGTACCCTTAATACTAGGAGCCCCTGATATAGCTTTTCCTCGAATAAATAATATAAGTTTTTGAATATTACCCCCCTCTTTAACACTGCTGCTTTCTAGTTCTATAGTAGAAAACGGAGCTGGAACAGGGTGAACGGTTTATCCTCCTCTTTCTTCTGGAACAGCTCATGCTGGGGCTTCAGTTGATTTAGCAATTTTTTCTTTACATTTAGCGGGAATCTCATCTATTTTAGGAGCAGTAAATTTTATTACAACTGTAATTAATATACGATCAACTGGTATTACTCTTGATCGACTACCTTTATTTGTGTGATCAGTAGTAATTACAGCTATTTTATTACTTCTTTCTCTACCCGTATTAGCCGGAGCTATTACTATATTATTAACAGACCGAAATTTAAATACATCTT,https://wolbachiaprojectdb.org/wp-content/uploads/2020/12/Luke_mosquito_COI-COI_F.fa,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/12/IMG_1915-rotated.jpg,",38.0041154000,-98.7600424000,11/19/2020,"<p>I purchased Triop eggs online and raised them. I was only able to get 3 to hatch out and those were the ones I extracted.<\/p>\n",Notostraca,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/12/Untitled-presentation.jpg,","<p>The Triops were raised for about 2 weeks until they reached full size. They were then stored in 90% ethanol for a day until extraction.<\/p>\n<p>DNA Extraction: The DNA was extracted from the abdomen.\u00a0 All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.<\/p>\n<p>PCR: Mini One Taq polymerase was used.<\/p>\n<p>Gel Electrophoresis: Mini PCR was used. All of the lanes worked well and were bright and clear.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/12/IMG_20201215_154333621-scaled.jpg,",38.9374808000,-77.0852258000,10/31/2020,"<p>I found this ladybug on my kitchen window at home.\u00a0 There were about 5-6 ladybugs trying to get into window from outside.<\/p>\n",Coleoptera,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/12/Luke-Dr-O-Gel.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/12/COI-and-Wolbachia-separate-gel.jpg,","<p>We first ran duplex PCR reaction (with both COI and <em>Wolbachia<\/em> primers) and then ran separate PCR reactions with just COI and just <em>Wolbachia<\/em> primers<\/p>\n",No,AGTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAGAGGAAGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTTTGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTTTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGATAAAACTCCTTTATTTGTATGATCTGTTCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTATTAACTGACCGAAACTTAAATTCTTCTTTTTTTGACCCAACCGGTGGGGGAGACCCAATTTTATACCAACATTTATTTTGATTTTTTG,https://wolbachiaprojectdb.org/wp-content/uploads/2020/12/Mod2.WspecF.fa,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/IMG_3927-scaled.jpg,",,,10/04/2020,"<p>When I caught the second bug it was so much easier. The bug as long legs like the first but this mosquitoes body was even smaller which makes me believe there is no Wolbachia in it.<\/p>\n",Culex,,,,,,"","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/IMG_3926-scaled.jpg,",,,10/04/2020,"<p>When I caught the mosquito it was more difficult than expected. Every time I went to go grab one it flew away. When I caught it I looked at it and the legs were very long and the body was very small.<\/p>\n",Culex,,,,,,"","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/IMG_1858-rotated.jpg,",38.0001781000,-98.7445481000,07/29/2020,"<p>The ticks were collected off of my dog during the late summer. They had not yet burrowed into the skin.<\/p>\n",Ixodida,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/Wolbachia-Project-3.jpg,","<p>The ticks were stored in 90% ethanol for about three months prior to extraction.<\/p>\n<p>DNA Extraction: The DNA was extracted from the abdomen.The abdomen was sliced open due to the tough exoskeleton. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.<\/p>\n<p>PCR: Mini One Taq polymerase was used.<\/p>\n<p>Gel Electrophoresis:\u00a0 Mini PCR was used. All of the lanes worked well and were bright and clear.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/IMG_1859-rotated.jpg,",38.0001701000,-98.7445612000,09/16/2020,"<p>The flea was captured off of my dog in mid September. He had been under some trees.<\/p>\n",Siphoneptera,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/Wolbachia-Project-3.jpg,","<p>The flea was stored in 90% ethanol for about two months prior to extraction.<\/p>\n<p>DNA Extraction: The DNA was extracted from the whole arthropod. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.<\/p>\n<p>PCR: Mini One Taq polymerase was used.<\/p>\n<p>Gel Electrophoresis:\u00a0 Mini PCR was used. All of the lanes worked well and were bright and clear.<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/fungusgnat33remote-scaled.jpeg,",42.5119345549,-71.3979608914,10/09/2020,"<p>The fungus gnat was collected\/found in a patch of grass in between mowed and unmowed lawn, so there were some well-maintained lawn and some dryer, yellow grass with weeds. There were also very few leaves in the area, and it was fairly open. There was also mulch a few feet away from the area. It was around 10:00 AM when the gnat was collected. It was about 65\u00ba F and cloudy.<\/p>\n",Mycetophilidae,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/02/Screen-Shot-2021-02-02-at-10.36.19-AM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2021/02/Screen-Shot-2021-02-02-at-10.36.06-AM.png,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/AMaqtedar4.jpeg,",,,,"<p>Relatively large Housefly<\/p>\n",Musca domestica,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/Screen-Shot-2020-11-09-at-10.08.46-AM.png,",42.4785882565,-71.4596435772,10/01/2020,"<p>Small Tick<\/p>\n",Ixodidae,,,,,,"","",,,,,
"",36.0775432,-86.7315785,09/24/2020,"<p>The beetle was found with many of the same species on a stump. It was extracted from the stump, which was damp and dark. It was 5:30 pm and around 70 degrees Fahrenheit.<\/p>\n",Megalodacne,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/2827557866911240542.jpg,",36.0775432000,-86.7315785000,,"<p>This arthropod was found underneath a rock in my front yard, along with several other insects of varying species. This was done in the afternoon, fairly warm out too but I do not have an exact measurement of the temperature. It was the only roly poly I managed to find. It was curled up, probably in an attempt to hide or protect itself from me, however, it had the opposite effect as I was able to grab it and submerge it in ethanol easier than if it were moving around.<\/p>\n",Armadillidae,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/Screenshot-2020-11-04-171526.png,","<p><a href=\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2020\/11\/Gel-Electrophoresis-Review-Cal-Neel.docx\">Gel Electrophoresis Review &#8211; Cal Neel<\/a><\/p>\n<p>My results are labeled under number 27 and 26. 27 was a common woodlouse and 26 was a carpenter ant. My pill bug tested positive for Wolbachia, however, my ant definitely did not.<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/IMG_1065-scaled.jpg,",36.1768873000,-86.7480349000,09/24/2020,"<p>This pavement ant was the second caught ant from my collection. It liked the sugar on my glove and was not frantic nor did it run away. It crawled around the glove until it was submerged in the ethanol. Had a thin black shell that covered the entirety of its body.<\/p>\n",Insecta,,,,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/Screenshot_8.png,","",No,,,,
"",,,11/04/2020,"<p>Very small fly<\/p>\n",Hexapoda,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/IMG_20201104_143819415_MP-scaled.jpg,",42.4836453,-71.4418101,10/02/2020,"<p>A black beetle thats length is 16mm<\/p>\n",,,,,,,"","",Unknown,,,,
"",,,01/01/2021,"<p>5 millimeters<\/p>\n",,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/IMG_7302.jpg,",,,,"<p>We found this spider in unmowed lawn near an open field. It was collected on October 2nd, 2020 in the afternoon. The area was surrounded by tall grasses and plants.<\/p>\n",Pholcidae,,,,,,"","",,,,,
"",42.47946491840872,-71.45769770902969,10/10/2020,"",Orthoptera,,,,,,"","",,,,,
"",37.3554104,-93.3993751,10/21/2020,"<p>found on concrete<\/p>\n<p>very fast compared to other insects in the vicinity<\/p>\n<p>72 degrees F<\/p>\n",Coleoptera,,Abdomen,,,,"","<p>abdomen was removed carefully with scissor tools<\/p>\n",Unknown,,,,
"",37.300994599999996,-93.4174773,10/21/2020,"<p>72 degrees F<\/p>\n<p>Field of tall grass<\/p>\n<p>was among many other grasshoppers, including many different species<\/p>\n<p>windy day<\/p>\n",Orthoptera,,Abdomen,,,,"","<p>Carefully removed abdomen with small scissor tool<\/p>\n",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/IMG-9453-scaled.jpg,",37.301,-93.417,10/22/2020,"",Orthoptera,,,,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/IMG-9444-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/IMG-9447-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/IMG-9451-scaled.jpg,","",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/damselfly-scaled.jpg,",37.301,-93.417,10/22/2020,"",Odonata,,,,,,"","",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/butterfly-scaled.jpg,",37.301,-93.417,12/23/2020,"",Lepidoptera,,,,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/IMG-9430-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/IMG-9439-scaled.jpg,","",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/BalaG1-scaled.jpg,",42.4801552477,-71.4567887381,10/01/2020,"<p>The leaf beetle was caught in the abandoned garden at the high school. The weather that week was sunny and ranged from 65-76 degrees F. It was one of the bigger insects caught in the garden, despite its small size.<\/p>\n",Chrysomelidae,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/Sophia1-scaled.jpg,",42.4777221504,-71.4584327922,10/01/2020,"<p>The spider was collected in a patch of woods in between the high school and a football field. It was sunny that week, with a low of 65 and a high of 76 degrees F. The spider was one of the largest insects collected in the cup, and seemed to be the only spider collected.<\/p>\n",Lycosidae,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/AAashi-5-scaled.jpg,",42.4788913244,-71.4546912505,10/01/2020,"<p>We set out cups of slightly soapy water with the openings flush to the ground. The ant was found in a grassy area close to a construction site.<\/p>\n",Lasius emarginatus,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG_2452.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG_2455.jpg,",37.3004,-93.4183,10/21/2020,"<p>Found in the grass, shaded by a tree. Temp outside was 75 degrees F<\/p>\n",Orthoptera,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/pgarro-2-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/220px-Oniscidea_woodlouse_morphology.png,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/download.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/images-1.jpeg,",42.4785594770,-71.4582102460,10/01/2020,"<p><img class=\"alignnone size-medium wp-image-1102\" src=\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2020\/10\/pgarro-2-146x300.jpg\" alt=\"\" width=\"146\" height=\"300\" \/><\/p>\n<p>Our group found only one sample of this species. The collection site was within a maintained\/ mowed area, which may have an impact on species collected as well.<\/p>\n",Armadillidium vulgare,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG_2447.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG_2444.jpg,",37.3004,-93.4183,10/21/2020,"<p>Found flying in tall grass, temp outside was 75 degrees F<\/p>\n",Diptera,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG_2433.jpg,",37.3004000000,-93.4183000000,10/21/2020,"<p>Found living under a heavy rock, the rock was cooler than the temperature outside. It was a sunny day, but the temperature outside was about 75 degrees F.<\/p>\n",Armadillidae,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG-4497-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG-4503-1-scaled.jpg,",37.3009789000,-93.4168276000,10/23/2020,"",Araneae,,,,,,"","",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/Mhu2insect-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/images.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/southern-yellowjacket-Vespula-squamosa.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/images-2.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/Bees-hornets-wasps_yellow-jacket_profile_350x350.jpg,",42.4785510637,-71.4590322504,10/01/2020,"<p><img class=\"alignnone size-medium wp-image-1096\" src=\"https:\/\/wolbachiaprojectdb.org\/wp-content\/uploads\/2020\/10\/Mhu2insect-225x300.jpg\" alt=\"\" width=\"225\" height=\"300\" \/><\/p>\n<p>Our group found this yellowjacket in an area that was not maintained (as the collection site was in the woods). We only collected one sample of many species, but were able to collect two samples of the Southern Yellowjacket.<\/p>\n",Vespula squamosa,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/rove-beetle.jpg,",37.301,-93.418,10/20/2020,"<p>We caught this in the field infront of the high school.<\/p>\n",Coleoptera,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/sac-spider-butt.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/sac-spider-mouth.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/sac-spider-whole-body-scaled.jpg,",39.2320000000,-93.3600000000,10/21/2020,"<p>This was also caught indoors, in Faith&#8217;s Livingroom.<\/p>\n",Araneae,,,,,,"","",,,,,
"",37.3013447,-93.4168724,10/22/2020,"<p>Outdoor temp: 82 degrees F<\/p>\n<p>Wasp was on Goldenrod flowers when caught<\/p>\n<p>Habitat: tall grass (field)<\/p>\n",Hymenoptera,,,,,,"","<p>We used a small scissor tool to carefully remove the abdomen from all other parts of the insect.<\/p>\n",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/red-paper-wasp-whole-body.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/red-paper-wasp-head.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/red-paper-wasp-stinger-.jpg,",37.301,-93.418,10/20/2020,"<p>This was found inside in WHS, in the hallways.<\/p>\n",Hymenoptera,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/Ezhong2-scaled.jpg,",42.4811823535,-71.4606381074,,"",Hemiptera,Puregene (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/02/1.jpg,","",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/Jzhang1-scaled.jpg,",42.4805656765,-71.4596952686,10/02/2020,"<p>The woods near leary<\/p>\n",Geotrupes splendidus,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2021/02/1-1.jpg,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/Resized_20201026_125118001.jpg,",37.3554104,-93.3993751,10/21/2020,"",Orthoptera,,Abdomen,,,,"","",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/large-cricket-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/Resized_20201026_123746001.jpg,",37.3554104000,-93.3993751000,10/21/2020,"",Orthoptera,,Abdomen,,,,"","",Unknown,,,,
"",37.30031042358681,-93.41824913863964,10/21/2020,"<p>Grass<\/p>\n<p>urbanized area<\/p>\n<p>mildly wet<\/p>\n",Diptera,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/grasshopper2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/grasshopper1.jpg,",37.3347932000,-93.4149334000,10/23/2020,"<p>It was caught in an open short grassy field.<\/p>\n",Orthoptera,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG_5253-scaled.jpg,",37.3010000000,-93.4160000000,10/21/2020,"<p>Web was sitting in tall grass, secluded field<\/p>\n",Araneae,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/Screenshot-2020-10-29-at-2.08.47-PM.png,",37.2990000000,-93.4200000000,10/21/2020,"",Lepidoptera,,,,,,"","",,,,,
"",37.3013447,-93.4168724,10/21/2020,"<p>Grasshopper was on a tall grass blade. Looked to be alone.<\/p>\n",Orthoptera,,,,,,"","",,,,,
"",37.3013447,-93.4168724,10/21/2020,"<p>Ant was on top of the ant hill. Hundreds of ants chased me as I scooped one up. The ant had wings.<\/p>\n",Hymenoptera,,,,,,"","",,,,,
"",37.3013447,-93.4168724,10/21/2020,"<p>We found the pill but under a wet log. There were at least 100 of them.<\/p>\n",Isopoda,,,,,,"","",,,,,
"",37.301,-93.418,10/12/2020,"",,,,,,,"","",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG-9549.jpg,",37.3013447,-93.4168724,10/22/2020,"",Orthoptera,,,,,,"","",Unknown,,,,
"",37.3554104,-93.3993751,10/22/2020,"",Hemiptera,,,,,,"","",,,,,
"",37.3554104,-93.3993751,10/21/2020,"",Orthoptera,,,,,,"","",,,,,
"",37.3010000000,-93.4180000000,10/12/2020,"",,,,,,,"","",Unknown,,,,
"",37.301,-93.418,10/12/2020,"",,,,,,,"","",Unknown,,,,
"",37.3010000000,-93.3010000000,10/21/2020,"",Diptera,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG-9453-1-scaled.jpg,",37.3013447,-93.4168724,10/22/2020,"",Orthoptera,,,,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG-9451-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG-9444-1-scaled.jpg,","",Unknown,,,,
"",37.3009698000,-93.4167642000,10/26/2020,"",Crustacea,,,,,,"","",,,,,
"",37.3009698,-93.41676419999999,10/26/2020,"",Crustacea,,,,,,"","",,,,,
"",37.3009698000,-93.4167642000,10/26/2020,"",Crustacea,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG-3208-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG-3211-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG-3215.jpg,",37.3009525000,-93.4167638000,10/21/2020,"<p>We collected this cricket near the school at around 1 pm. It was about 70 degrees outside and we collected this cricket along with one other from a group of crickets.<\/p>\n<p>iNaturalist: luke_christensen 73<\/p>\n",Gryllus,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/7.Blue_.SH_.Woody_.Beetle-scaled.jpg,",42.4850931000,-71.4328400000,11/29/2020,"<p>Found in wooded environment<\/p>\n",Geotrupes splendidus,,,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/butterfly-scaled.jpg,",37.3013447,-93.4168724,10/22/2020,"",Lepidoptera,,Abdomen,,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG-9430-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG-9439-1-scaled.jpg,","",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG-3221-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG-3225-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG-3227-scaled.jpg,",37.3009525000,-93.4167638000,10/21/2020,"<p>We found the field cricket near the school. It was collected in the afternoon around 1 pm, and it was around 70 degrees outside. There was more than one cricket, but we collected this one and one other.<\/p>\n<p>&nbsp;<\/p>\n<p>iNaturalist: luke_christensen 74<\/p>\n",Gryllus,,,,,,"","",,,,,
"",38.0001790000,-98.7445536000,08/19/2020,"<p>The larvae were found in sandy soil in their pits. There were a lot of other pits around. The adult was found in my backyard.<\/p>\n",Neuroptera,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/Wolbachia-Project-2.jpg,","<p>I stored my arthropods in 90% ethanol for about two months prior to DNA extraction.<\/p>\n<p>DNA extraction: The DNA was extracted from the abdomen. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.<\/p>\n<p>PCR: Mini One Taq polymerase was used.<\/p>\n<p>Gel Electrophoresis: All of the lanes worked except for the DNA ladder and the positive and negative controls. This may be due to a pipetting error. Importantly though my Wolbachia DNA worked.<\/p>\n<p>&nbsp;<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/FED6693B-AD53-4266-B611-2879DC5F027C.jpeg,",36.0802326000,-86.8320743000,09/24/2020,"<p>Found at the foot of a tree. There were several smaller ants nearby.<\/p>\n",Formicidae,DNeasy (Qiagen),Whole arthropod,,,,"","",,,,,
"",47.1600000000,8.4300000000,09/28/2020,"<ul>\n<li>Collected from a pond<\/li>\n<li>Humid<\/li>\n<li>Autumn<\/li>\n<li>13\u00b0C<\/li>\n<li>Elevation \u2013 538<\/li>\n<\/ul>\n",Cicadella viridis,Wizard SV (Promega),,,,,"","",No,TCTGGATGGTTGGGATAATACTAAGAATAATTATTCGAATTGAATTAGCTCAACCAGGATCATTTATTCTCAACGATCAAGTTTATAATGTAATTGTTACATCACATGCATTTATCATAATTTTTTTTATAGTTATACCTATTATAATTGGTGGTTTTGGTAATTGATTATTACCTTTAATAATTGGTGCCCCGGATATAGCATTTCCTCGAATAAATAATATAAGATTTTGACTTTTACCACCTTCATTAATTTTACTTATAACTAGTTCAATAGTTGAAATAGGAGCAGGAACCGGTTGAACAGTTTATCCCCCTTTATCATCTAATATTGCACATTCAGGTTCAAGAGTTGATTTAGCAATTTTTTCTTTACATTTAGCTGGTATTTCATCTATTTTAGGTGCAGTTAATTTTATTACAACTGTAATTAATATGCGAATACTAGGTATAAATTTTGATCAAACACCATTGTTTGTTTGATCAGTTATAATTACTGCTATTCTTTTATTACTATCTTTACCAGTTTTAGCTGGTGCTATTACTATATTATTAACAGATCGTAATTTAAATACATCTTTTTTTGACCCCTCAGGTGGTGGTGATCCAATTTTATATCAACATTTATTTTGATTTTTTGGTCACC,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C024.fasta,,
"",47.1600000000,8.4300000000,09/28/2020,"<ul>\n<li>Collected from a field with cows<\/li>\n<li>Autumn<\/li>\n<li>13\u00b0C<\/li>\n<li>Elevation \u2013 538<\/li>\n<\/ul>\n",Scathophaga stercoraria,Wizard SV (Promega),,,,,"","",No,TGATCCGGATAATTGGAACTTCTTTAAGTATTCTAATTCGAGCTGAATTAGGACATCCTGGAGCATTAATTGGAGATGACCAAATCTATAATGTAATTGTAACAGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGATTTGGAAATTGATTAGTGCCTCTTATATTAGGTGCTCCTGATATAGCTTTCCCTCGAATAAATAATATAAGTTTTTGACTTTTACCACCAGCATTAACTTTATTGTTAGTAAGTAGTATAGTAGAAAACGGAGCTGGAACAGGATGAACTGTTTACCCTCCTTTATCTTCAAATATTGCTCATGGAGGAGCTTCTGTTGATTTAGCAATTTTTTCTCTTCATTTAGCAGGAATTTCATCTATTTTAGGAGCTGTAAATTTTATTACAACTGTAATTAATATACGATCAACAGGAATTACTTTTGATCGAATACCTTTATTTGTATGATCTGTAGTAATTACAGCTTTATTACTTTTATTATCTTTACCTGTTTTAGCTGGAGCTATTACTATACTATTAACAGATCGAAATCTTAATACATCATTTTTTGATCCAGCAGGAGGAGGAGACCCAATTCTATATCAACATTTATTTTGATTTTTTGGTCACCGGGAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C023.fasta,,
"",47.1600000000,8.4300000000,09/28/2020,"<ul>\n<li>Collected from the woods<\/li>\n<li>Autumn<\/li>\n<li>13\u00b0C<\/li>\n<li>Elevation \u2013 538<\/li>\n<\/ul>\n",Loricera pilicornis,Wizard SV (Promega),,,,,"","",Yes,CAGGATAGTGGGAACCTCCCTAAGAATGTTAATTCGAGCAGAATTAGGGAATCCAGGTTCTTTTATTGGAGATGATCAAATTTATAATGTTATTGTTACTGCTCATGCATTTGTAATGATTTTTTTTATAGTTATACCAATTATAATTGGAGGATTTGGAAACTGACTTGTTCCTTTAATATTAGGTGCCCCTGATATAGCCTTTCCTCGAATAAATAATATAAGATTTTGACWCCTTCCACCATCTCTTACTCTTCTCCTACTTAGAAGAATGGTAGAGAGAGGTGCTGGTACAGGATGGACCGTGTACCCTCCTCTTTCTTCTGTTATTGCTCATAGAGGGGCTTCAGTAGATTTAGCTATTTTTAGTCTTCACTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTAGTACAATTATTAATATACGATCTATTGGAATGACATTTGATCGAATACCACTATTTGTTTGATCAGTGGGAATTACTGCTCTACTTCTTTTACTTTCGTTACCAGTATTAGCTGGAGCAATTACTATATTATTAACTGATCGAAATTTAAATACATCCTTTTTTGATCCGGCGGGAGGAGGAGATCCTATTCTATATCAACATTTATTTTGATTTTTTGGTCACCTGGAAAGTTTAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C022.fasta,,
"",47.1600000000,8.4300000000,,"<ul>\n<li>Collected from the woods<\/li>\n<li>Autumn<\/li>\n<li>13\u00b0C<\/li>\n<li>Elevation \u2013 538<\/li>\n<\/ul>\n",Loricera pilicornis,Wizard SV (Promega),,,,,"","",Yes,CAGGATAGTGGGACCTCCCTAAGAATGTTAATTCGAGCAGAATTAGGGAATCCAGGTTCTTTTATTGGAGATGATCAAATTTATAATGTTATTGTTACTGCTCATGCATTTGTAATGATTTTTTTTATAGTTATACCAATTATAATTGGAGGATTTGGAAACTGACTTGTTCCTTTAATATTAGGTGCCCCTGATATAGCCTTTCCTCGAATAAATAATATAAGATTTTGACTCCTTCCACCATCTCTTACTCTTCTCCTACTTAGAAGAATGGTAGAGAGAGGTGCTGGTACAGGATGGACCGTGTACCCTCCTCTTTCTTCTGTTATTGCTCATAGAGGGGCTTCAGTAGATTTAGCTATTTTTAGTCTTCACTTARCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTAGTACAATTATTAATATACGATCTATTGGAATGACATTTGATCGAATACCACTATTTGTTTGATCAGTGGGAATTACTGCTCTACTTCTTTTACTTTCGTTACCAGTATTAGCTGGAGCAATTACTATATTATTAACTGATCGAAATTTAAATACATCCTTTTTTGATCCGGCGGGAGGAGGAGATCCTATTCTATATCAACATTTATTTTGATTTTTTGGTCACCCTGGAAGTTTAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C021.fasta,,
"",47.1600000000,8.4300000000,09/28/2020,"<ul>\n<li>Collected from underneath a stone<\/li>\n<li>Autumn<\/li>\n<li>13\u00b0C<\/li>\n<li>Elevation \u2013 538<\/li>\n<\/ul>\n",Trachelipus,Wizard SV (Promega),,,,,"","",No,TGGTGCTTGAGCAGGGGCTGTTGGAACAGCCCTTAGGATAATTATTCGGACTGAATTAGGAAACCCTGGAAGGTTAATTGGAGATGATCAGATTTATAATGTTATTGTGACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGTTTCGGTAATTGATTAGTGCCTTTAATATTAGGGGCCCCTGATATAGCTTTTCCACGTATAAATAATATAAGGTTTTGGCTTCTCCCCCCATCACTAATCTTGTTATTAAGAAGGGGTCTAGTTGAAAGAGGGGTTGGGACTGGCTGGACTGTCTACCCACCTTTGGCAAGTGGAATCGCCCATAGAGGGGCTTCAGTAGATTTAGGGATTTTTTCCCTTCATTTGGCTGGAGCTTCTTCAATCTTAGGAGCTGTAAATTTTATTACTACTGTAATTAATATACGAGCAGAGGGGATAAGGATAGATCGTATTCCTCTATTTGTCTGGTCTGTGTTAATTACAGCAATTCTATTATTACTTTCACTACCGGTTTTAGCAGGGGCTATTACTATATTATTAACAGACCGTAATCTGAACACCTCTTTTTTTGACCCTAGGGGGGGAGGGGATCCTATCTTATACCAGCACTTATTTTGATTTTTTGGTCACCCTGAAAGTTTAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C020.fasta,,
"",47.1600000000,8.4300000000,09/28/2020,"<ul>\n<li>Collected from underneath a stone<\/li>\n<li>Autumn<\/li>\n<li>13\u00b0C<\/li>\n<li>Elevation \u2013 538<\/li>\n<\/ul>\n",Trachelipus,Wizard SV (Promega),,,,,"","",No,GCTGTTGGAACAGCCCTTAGGATAATTATTCGGACTGAATTAGGAAACCCTGGAAGGTTAATTGGAGATGATCAGATTTATAATGTTATTGTGACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGTTTCGGTAATTGATTAGTGCCTTTAATATTAGGGGCCCCTGATATAGCTTTTCCACGTATAAATAATATAAGGTTTTGGCTTCTCCCCCCATCACTAATCTTGTTATTAAGAAGGGGTCTAGTTGAAAGAGGGGTTGGGACTGGCTGGACTGTCTACCCACCTTTGGCAAGTGGAATCGCCCATAGAGGGGCTTCAGTAGATTTAGGGATTTTTTCCCTTCATTTGGCTGGAGCTTCTTCAATCTTAGGAGCTGTAAATTTTATTACTACTGTAATTAATATACGAGCAGAGGGGATAAGGATAGATCGTATTCCTCTATTTGTCTGGTCTGTGTTAATTACAGCAATTCTATTATTACTTTCACTACCGGTTTTAGCAGGGGCTATTACTATATTATTAACAGACCGTAATCTGAACACCTCTTTTTTTGACCCTAGGGGGGGAGGGGATCCTATCTTATACCAGCACTTATTTTGATTTTTTGGTCACCTGGAAAGTTTAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C019.fasta,,
"",,,09/28/2020,"",Autographa gamma,Wizard SV (Promega),,,,,"","",Yes,CTTTAAGATTACTAATTCGAGCAGAATTAGGAACCCCTGGATCATTAATTGGTGATGATCAAATTTATAATRCTATTGTTACAGCTCATGCATTTATTATAATTTTTTTTATAGTTATGCCTATTATAATTGGAGGATTTGGTAATTGACTCGTTCCTCTAATATTAGGAGCTCCTGATATAGCTTTCCCTCGTATAAATAACATAAGTTTTTGACTTTTACCCCCATCTTTAACTCTTTTAATTTCTAGAAGAATTGTAGAAAATGGAGCTGGTACTGGATGAACAGTTTATCCCCCACTTTCATCTAATATCGCCCATGGTGGAAGATCTGTTGATTTATCTATTTTTTCTTTACATTTAGCTGGAATTTCATCAATTTTAGGAGCAATTAATTTTATTACAACAATTATTAATATACGATTAAATAGTTTATCTTTTGATCAAATACCTTTATTTATCTGAGCTGTTGGAATTACAGATTTCCTTTTATTACTTTCTTTACCAGTTTTAGCAGGGGCAATTACTATATACTTTTAACAGATCGAAATTTAAATACTTCTTTTTTTGATCCTGCTGGAGGAGGAGACCCAATCTTATACCAACATTTATTTTGCTTTTTTGGTCACC,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C018_TR-1.fasta,,
"",,,09/28/2020,"",Autographa gamma,Wizard SV (Promega),,,,,"","",Yes,GGTATTTGAGCTGGAATAGTTGGTACATCTTTAAGATTACTAATTCGAGCAGAATTAGGAACCCCTGGATCTTTAATTGGTGATGATCAAATTTATAATACTATTGTTACAGCTCATGCATTTATTATAATTTTTTTTATAGTTATGCCTATTATAATTGGAGGATTTGGTAATTGACTCGTTCCTCTAATATTAGGAGCTCCTGATATAGCTTTCCCTCGTATAAATAACATAAGTTTTTGACTTTTACCCCCATCTTTAACTCTTTTAATTTCTAGAAGAATTGTAGAAAATGGAGCTGGTACTGGATGAACAGTTTATCCCCCACTTTCATCTAATATCGCCCATGGTGGAAGATCTGTTGATTTAGCTATTTTTTCTTTACATTTAGCTGGAATTTCATCAATTTTAGGAGCAATTAATTTTATTACAACAATTATTAATATACGATTAAATAGTTTATCTTTTGATCAAATACCTTTATTTATCTGAGCTGTTGGAATTACAGCTTTCCTTTTATTACTTTCTTTACCTGTTTTAGCAGGGGCAATTACTATACTTTTAACAGATCGTAATTTAAATACTTCTTTTTTTGATCCTGCTGGAGGAGGAGACCCAATCTTATACCAACATTTATTTTGATTTTTTGGTCACCTGGAGAA,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C017.fasta,,
"",,,09/28/2020,"",Pentatoma rufipes,Wizard SV (Promega),,,,,"","",No,ATATGAGCAGGTATAGTAGGATCAGCTATAAGTATTATTATTCGAATTGAATTAGGTCAACCCGGAAGATTTATTGGAGATGATCAAATTTATAATGTTGTAGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATGCCAATTATAATTGGTGGGTTTGGAAATTGACTAGTACCTTTAATAATTGGAGCACCTGATATAGCCTTTCCACGAATAAATAATATAAGATTTTGATTATTACCCCCTTCATTAACATTATTAATAATAAGAAGATTAACAGAAGCTGGAGCTGGTACTGGCTGAACAGTTTACCCCCCTTTATCTAGAAATTTATCACACAGAGGAACTTCAGTAGATTTAGCTATTTTTTCTCTTCATTTAGCAGGGGTTTCTTCAATTTTAGGTGCAGTAAATTTCATTTCAACAATTATAAATATACGCCCAACTGGAATAACTCCCGAGCGAATTCCTCTATTCGTTTGATCTGTTGGAATTACAGCTTTACTTTTATTGTTATCACTACCAGTATTAGCTGGAGCTATTACAATATTATTAACAGATCGAAATTTTAATACATCATTTTTTGACCCCTCAGGGGGTGGGGATCCCATTCTTTATCAACATTTATTTTGATTTTTTGGTCACCTG,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C014.fasta,,
"",,,09/28/2020,"",Phoridae,Wizard SV (Promega),,,,,"","",No,GAGTCNTGATGGTGGGCACATCTTAAGAGTATGTAATTCGTGCTGAATTAGGGCGCCCCGGGCTTTATTTGGAGATGATCAAATTGATAATGTAATTTG,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C013_TR.fasta,,
"",47.2400000000,8.3100000000,,"<ul>\n<li>Collected from the grass in an open, sunny field<\/li>\n<li>Autumn<\/li>\n<li>15\u00b0C<\/li>\n<li>Elevation \u2013 571<\/li>\n<\/ul>\n",Vespula germanica,Wizard SV (Promega),,,,,"","",No,GAGCAGGACTTTAGGAGCTTCAATAAGAATAATTATTCGTTTAGAATTAAGATCCCCTGGAGCTTTAATTAATAATGATCAAATTTATAATACTATTATTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTTATACCTTTTTTAGTTGGAGGATTTGGAAATTGATTAATCCCTTTAATATTAGGTGTTCCTGATATAGCATTTCCTCGAATAAATAATATAAGATTTTGATTATTACCTCCATCCTTATTTTTATTAATCTTAAGAAATTTTATTGGAACCGGAGTAGGGACAGGATGAACTCTATACCCTCCTTTATCATCAATTGTAGGACATGACTCTCCCTCTGTAGACTTAGGAATTTTTTCTATCCATATTGCTGGAATTTCATCAATTATAGGTTCAATTAATTTTATTGTTACTATTTTAAATATACACACAAAAACACATTCACTAAATTTTCTTCCTTTATTTTCATGATCAATTTTAATTACAGCAATTCTTCTCTTGTTATCTCTACCAGTTCTTGCAGGAGCAATTACTATACTTCTTACAGACCGTAATTTAAATACATCTTTCTTTGATCCTGCAGGCGGAGGTGACCCAATTTTATATCAACATTTATTTTGATTTTTTGGTCACTG,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C012.fasta,,
"",47.2300000000,8.3200000000,09/28/2020,"<ul>\n<li>Collected from a flower<\/li>\n<li>Autumn<\/li>\n<li>15\u00b0C<\/li>\n<li>Elevation \u2013 460<\/li>\n<\/ul>\n",Nezara viridula,Wizard SV (Promega),,,,,"","",No,GGTATATGAGCAGGAATAGTAGGATCAGCAATAAGATTAATTATTCGAATTGAACTAGGACAACCCGGAAGATTTATTGGAGATGATCAAATTTATAATGTAGTAGTAACAGCTCACGCATTTGTAATAATTTTCTTTATAGTAATACCAATCATAATTGGAGGATTTGGAAATTGATTAGTACCTTTAATAATTGGTGCTCCAGATATAGCATTCCCTCGAATAAACAATATAAGATTCTGGTTATTACCCCCATCATTAACCCTTTTAATAGTAAGAAGATTAGCAGAATCTGGAGCAGGAACAGGATGAACTGTTTATCCTCCTTTATCTAGTAATTTATCTCATAGAGGAGCCTCAGTGGATTTAGCTATTTTTTCATTACATCTAGCAGGAGTATCATCAATCTTAGGCGCAGTAAATTTCATTTCAACTATTATTAATATACGACCAACAGGTATAACTCCAGAACGAGTTCCACTATTTGTTTGATCAGTTGGGATCACAGCATTATTATTACTACTTTCATTACCTGTACTAGCAGGTGCAATTACAATATTATTAACAGATCGGAACTTTAATACATCATTCTTTGACCCTTCAGGAGGGGGAGATCCCATTCTTTATCAACACTTATTTTGATTTTTTGGTCACCTGAG,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C011.fasta,,
"",47.2400000000,8.3100000000,09/28/2020,"<ul>\n<li>Collected from dry, short grass in a meadow<\/li>\n<li>Autumn<\/li>\n<li>15\u00b0C<\/li>\n<li>Elevation \u2013 571<\/li>\n<\/ul>\n",Orthoptera,Wizard SV (Promega),,,,,"","",Yes,ATGAGCTGGATAGTAGGTACATCAATAAGAATAATTATTCGTGCAGAACTTGGACAACCAGGATCCATAATTGGAGATGATCAAATCTATAATGTTATTATTACAGCACATGCATTTGTAATAATTTTCTTCATAGTAATACCTATTATAATTGGGGGATTCGGTAATTGACTGGTTCCACTAATAATCGGAGCACCAGATATAGCTTTTCCACGAATAAATAACATAAGTTTTTGACTTTTACCACCATCATTAACTCTTTTAATTGCATCATCAATAGTAGATAATGGTGCAGGAACAGGATGAACAGTTTATCCCCCTCTCGCAGGAGCAATCGCACATGGAGGAGGATCAGTAGACCTGGCTATTTTTTCATTACATTTAGCAGGTGTTTCATCAATTTTAGGAGCAGTTAATTTCATTACAACTGCAATCAATATACGGTCGGAAAGAATAACACTAGATCAAACACCACTATTTGTCTGATCAGTAGCAATTACAGCACTCCTATTACTATTATCATTACCGGTACTAGCAGGAGCAATTACTATATTATTAACTGATCGAAATTTAAATACTTCGTTCTTTGACCCCGCAGGTGGTGGAGATCCAATCCTTTATCAACATTTATTTTGATTTTTTGGTCACCTGGAG,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C010.fasta,,
"",47.2300000000,8.3200000000,09/28/2020,"<ul>\n<li>Collected from the road<\/li>\n<li>Autumn<\/li>\n<li>15\u00b0C<\/li>\n<li>Elevation \u2013 460<\/li>\n<\/ul>\n",Pentatoma rufipes,Wizard SV (Promega),,,,,"","",No,TATATGAGCAGGTATAGTAGGATCAGCTATAAGTATTATTATTCGAATTGAATTAGGTCAACCCGGAAGATTTATTGGAGATGATCAAATTTATAATGTTGTAGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATGCCAATTATAATTGGTGGGTTTGGAAATTGACTAGTACCTTTAATAATTGGAGCACCTGATATAGCCTTTCCACGAATAAATAATATAAGATTTTGATTATTACCCCCTTCATTAACATTATTAATAATAAGAAGATTAACAGAAGCTGGAGCTGGTACTGGCTGAACAGTTTACCCCCCTTTATCTAGAAATTTATCACACAGAGGAACTTCAGTAGAYTTAGCTATTTTTTCTCTTCATTTAGCAGGGGTTTCTTCAATTTTAGGTGCAGTAAATTTCATTTCAACAATTATAAATATACGCCCAACTGGAATAACTCCCGAGCGAATTCCTCTATTCGTTTGATCTGTTGGAATTACAGCTTTACTTTTATTGTTATCACTACCAGTATTAGCTGGAGCTATTACAATATTATTAACAGATCGAAATTTTAATACATCATTTTTTGACCCCTCAGGGGGTGGGGATCCCATTCTTTATCAACATTTATTTTGATTTTTTGGTCACCCTGGAAAGTTTA,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C009.fasta,,
"",47.24,8.31,09/28/2020,"<ul>\n<li>Collected from grass, field (terrestrial)<\/li>\n<li>Autumn<\/li>\n<li>15\u00b0C<\/li>\n<li>Elevation \u2013 571<\/li>\n<\/ul>\n",Orthoptera,Wizard SV (Promega),,,,,"","",Yes,ATGAGCTGGATAGTAGGTACATCAATAAGAATAATTATTCGTGCAGAACTTGGACAACCAGGATCCATAATTGGAGATGATCAAATCTATAATGTTATTATTACAGCACATGCATTTGTAATAATTTTCTTCATAGTAATACCTATTATAATTGGAGGATTCGGTAATTGACTGGTTCCACTAATAATCGGAGCACCAGATATAGCTTTTCCACGAATAAATAACATAAGTTTTTGACTTTTACCACCATCATTAACTCTTTTAATTGCATCATCAATAGTAGATAATGGTGCAGGAACAGGATGAACAGTTTATCCCCCTCTCGCAGGAGCAATCGCACATGGAGGAGGATCAGTAGACCTGGCTATTTTTTCATTACATTTAGCAGGTGTTTCATCAATTTTAGGAGCAGTTAATTTCATTACAACTGCAATCAATATACGGTCGGAAAGAATAACACTAGATCAAACACCACTATTTGTCTGATCAGTAGCAATTACAGCACTCCTATTACTATTATCATTACCGGTACTAGCAGGAGCAATTACTATATTATTAACTGATCGAAATTTAAATACTTCGTTCTTTGACCCTGCGGGTGGTGGAGATCCAATCCTTTATCAACATTTATTTTGATTTTTTGGTCACCCTGGAAGTTTAATC,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C008.fasta,,
"",47.2400000000,8.3100000000,09/28/2020,"<ul>\n<li>Collected from forest (terrestrial)<\/li>\n<li>Autumn<\/li>\n<li>15\u00b0C<\/li>\n<li>Elevation \u2013 571<\/li>\n<\/ul>\n",Harmonia axyridis,Wizard SV (Promega),,,,,"","",No,GGAATAGTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAGAGGAAGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTTTGGAAATTGATTA,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C006.fasta,,
"",47.2400000000,8.3100000000,09/28/2020,"<ul>\n<li>Terrestrial<\/li>\n<li>Autumn<\/li>\n<li>15\u00b0C<\/li>\n<li>Elevation \u2013 571<\/li>\n<\/ul>\n",Nezara viridula,Wizard SV (Promega),,,,,"","",No,GAGCAGGATAGTAGGATCAGCAATAAGATTAATTATTCGAATTGAACTAGGACAACCCGGAAGATTTATTGGAGATGATCAAATTTATAATGTAGTAGTAACAGCTCACGCATTTGTAATAATTTTCTTTATAGTAATACCAATCATAATTGGAGGATTTGGAAATTGATTAGTACCTTTAATAATTGGTGCTCCAGATATAGCATTCCCTCGAATAAACAATATAAGATTCTGGYTATTACCCCCATCATTAACCCTTTTAATAGTAAGAAGATTAGCAGAATCTGGAGCAGGAACAGGATGAACTGTTTATCCTCCTTTATCTAGTAATTTATCTCATAGAGGAGCCTCAGTGGATTTAGCTATTTTTTCATTACATCTAGCAGGAGTATCATCAATCTTAGGCGCAGTAAATTTCATTTCAACTATTATTAATATACGACCAACAGGTATAACTCCAGAACGAGTTCCACTATTTGTTTGATCAGTTGGGATCACAGCATTATTATTACTACTTTCATTACCTGTACTAGCAGGTGCAATTACAATATTATTAACAGATCGGAACTTTAATACATCATTCTTTGACCCTTCAGGAGGGGGAGATCCCATTCTTTATCAACACTTATTTTGATTTTTTGGTCACCCTGAAAGTTTAAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C005.fasta,,
"",47.2300000000,8.3200000000,,"<ul>\n<li>Collected from dark, dry area, under a building<\/li>\n<li>Autumn<\/li>\n<li>15\u00b0C<\/li>\n<li>Elevation \u2013 460<\/li>\n<\/ul>\n",Pyrrhocoris apterus,Wizard SV (Promega),,,,,"","",No,GGTATATGATCAGGAATAATTGGATCCTCAATAAGTTGAATTATTCGAGTAGAACTAGGACAACCTGGAACCTTTATTGGAGATGATCAAATTTATAATGTAATCGTCACAGCACATGCTTTCATTATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGCAATTGATTAGTTCCTTTAATAATTGGAGCACCAGATATAGCTTTTCCTCGCATAAATAATATAAGATTTTGACTTTTACCACCCTCTCTAACTCTATTAATCTCCAGTAGCATAGTAGAAATAGGAGCAGGAACTGGTTGAACAGTTTACCCGCCTTTATCTAGTAATATCTCACATAGAGGACCTTCAGTAGATTTAGCAATCTTTTCATTACATTTAGCAGGAGTTTCTTCAATTCTAGGAGCAGTTAATTTTATTTCAACAATTATTAACATACGACCTATAGGGATAACTCTTGAACGAACACCATTATTTGTTTGATCAGTAGGAATTACTGCTTTATTATTACTTTTATCATTACCAGTTCTAGCAGGAGCCATTACAATACTATTAACTGATCGAAATTTTAATACCTCTTTCTTTGACCCTACAGGAGGAGGAGACCCAATTCTTTATCAACATCTATTTTGATTTTTTGGTCACCGGGGAA,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C004.fasta,,
"",47.2400000000,8.3200000000,09/28/2020,"<ul>\n<li>Collected from forest, air<\/li>\n<li>Autumn<\/li>\n<li>15\u00b0C<\/li>\n<li>Elevation \u2013 460<\/li>\n<\/ul>\n",Athalia cordata,Wizard SV (Promega),,,,,"","",No,CAGGATACTTGGACTATCACTAAGCATGATTATCCGAAGAGAATTAAGATCAGCAAATTCACTTATTAATAATGATCAAGTTTATAACGTAATTGTTACATCTCACGCTTTCTTAATAATTTTCTTCATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGATTAATTCCATTAATATTAGGAGCACCCGATATAGCATTCCCACGAATAAATAATATAAGATTCTGATTTTTACCTCCATCCCTAATTCTACTTCTTTCAAGAAGAATAGTAAACACTGGATCTGGAACAGGGTGAACAGTTTATCCACCATTATCCTCAAATACCTCCCATGCTGGAGCATCTGTTGATTTAACAATTTTTTCATTACATTTAGCAGGTATTTCTTCCATTTTAGGAGCTATTAACTTTATTTCTACTATTTTAAATATACGAATAAATGGTATAACAAACGAACGAATAAGTTTATTCACATGATCAGTATTATTAACAGCTATTTTATTATTATTATCATTACCAGTGCTAGCAGGAGCAATTACAATATTATTAACAGATCGAAATTTAAATACATCATTTTTTGACCCTTCTGGAGGTGGAGACCCCATTCTATATCAACATCTATTTTGATTTTTTGGTCAC,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C003.fasta,,
"",47.2400000000,8.3100000000,09/28/2020,"<ul>\n<li>Collected from grass, field (terrestrial)<\/li>\n<li>Autumn<\/li>\n<li>15\u00b0C<\/li>\n<li>Elevation \u2013 571<\/li>\n<\/ul>\n",Voria ruralis,Wizard SV (Promega),,,,,"","",No,AGGATAGTAGGAACATCCCTTAGAATATTAATTCGATTTGAATTAGGTCATCCAGGAGCCTTAATTGGTAATGATCAAACATACAATGTTATCGTTACTGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGATTTGGAAATTGATTAGTTCCTCTTATACTAGGAGCTCCTGATATAGCTTTTCCTCGAATAAATAATATAAGTTTTTGATTACTTCCTCCTTCACTAACATTATTATTGATAAGAAGAATGGTTGAAAACGGAGCTGGAACAGGATGAACTGTTTATCCACCTTTATCATCTAATATTGCTCATAGAGGATCTTCTGTTGATTTAGCTATTTTTTCATTACATTTAGCTGGAATTTCATCAATTTTGGGAGCTGTAAATTTTATTACTACAGTAATTAATATACGATCTGTAGGAATTACATTTGATCGAATACCATTATTTGTTTGATCAGTAGTAATTACTGCATTATTACTTTTATTATCATTACCCGTATTAGCTGGAGCTATTACTATACTATTAACAGATCGAAATTTTAATACTTCTTTTTTTGATCCTGCTGGAGGAGGAGATCCTATTCTTTATCAACATTTATTTTGATTTTTTGGTCAC,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C002.fasta,,
"",47.2400000000,8.3100000000,09/28/2020,"<ul>\n<li>Collected from grass, field (terrestrial)<\/li>\n<li>Autumn<\/li>\n<li>15\u00b0C<\/li>\n<li>Elevation &#8211; 571<\/li>\n<\/ul>\n",Nezara viridula,Wizard SV (Promega),,,,,"","",No,TATGAGCAGGAATAGTAGGATCAGCAATAAGATTAATTATTCGAATTGAACTAGGACAACCCGGAAGATTTATTGGAGATGATCAAATTTATAATGTAGTAGTAACAGCTCACGCATTTGTAATAATTTTCTTTATAGTAATACCAATCATAATTGGAGGATTTGGAAATTGATTAGTACCTTTAATAATTGGTGCTCCAGATATAGCATTCCCTCGAATAAACAATATAAGATTCTGGTTATTACCCCCATCATTAACCCTTTTAATAGTAAGAAGATTAGCAGAATCTGGAGCAGGAACAGGATGAACTGTTTATCCTCCTTTATCTAGTAATTTATCTCATAGAGGAGCCTCAGTGGATTTAGCTATTTTTTCATTACATCTAGCAGGAGTATCATCAATCTTAGGCGCAGTAAATTTCATTTCAACTATTATTAATATACGACCAACAGGTATAACTCCAGAACGAGTTCCACTATTTGTTTGATCAGTTGGGATCACAGCATTATTATTACTACTTTCATTACCTGTACTAGCAGGTGCAATTACAATATTATTAACAGATCGGAACTTTAATACATCATTCTTTGACCCTTCAGGAGGGGGAGATCCCATTCTTTATCAACACTTATTTTGATTTTTTGGTCACCTGGGAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/EDU150_C001.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG_1515.jpg,",36.0802326,-86.8320743,09/24/2020,"<p>Black\/very dark brown. Wings. 6 long legs\/limbs. 2 limbs are striped.<\/p>\n",Culicidae,,Partial abdomen,,,,"","",,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/2528A369-A969-4487-8412-1B0F5539FBE8-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/7B0D2CB7-15CC-4E6B-8F63-BACBE0159D77-scaled.jpeg,",36.2754942000,-86.8320743000,09/25/2020,"<p>I found this ant in my back yard behind some rocks. The temperature kind of felt humid that day, if I had to guess the temperature I would say it was around 70-75 degrees F. When collecting the ant it seemed to be slower than most but it was able to dodge my trap by weaving through the rocks.<\/p>\n",Insecta,Edwards Buffer,Abdomen,,,,"","<p>For the DNA extraction part of this I sent the insect into a lab and so far I haven\u2019t gotten back any results.<\/p>\n",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/ant-on-wood.jpg,",36.1020038000,-87.1150054000,09/24/2020,"<p>I collected this ant from under old damp wood. There were many more ants in this environment along with many other insect species. This ant was collected at around 5:30 pm and it was in the lower 70s outside.<\/p>\n",Formicidae,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/Screenshot-2020-11-12-145209.png,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/ant-on-glass.jpg,",36.10200380000001,-87.1150054,09/24/2020,"<p>I collected this ant from under old wet pieces of wood found in the woods. I collected this ant at around 6 pm and it was in the lower 70s outside. There was an abundance of these ants along with others in this environment.<\/p>\n",Formicidae,DNeasy (Qiagen),Whole arthropod,,,,"","",Unknown,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/pill-bug.jpg,",36.1020038000,-87.1150054000,09/24/2020,"<p>I found this pill bug\/rolly polly under a wet flower pot. It was collected in the mid-afternoon around 4:20 pm. It was in the higher 60s lower 70s outside. There were lots of rolly pollies in this area, as well as under wet leaves and old wood.<\/p>\n",Armadillidae,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/Screenshot-2020-11-12-145209-1.png,","",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/IMG-0416-scaled.jpeg,",36.0734056825,-86.7488168042,09/24/2020,"<p>The Red-banded Fungus Beetle was living in a tree stump with many of its same variety. I collected 5 of these. It was completely submerged in the wood, which was damp. It&#8217;s overall environment was dark. It was collected at 5:30 pm and the temperature outside was about 70 degrees.<\/p>\n",Megalodacne,Puregene (Qiagen),Partial abdomen,Standard electrophoresis system,TAE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/Screenshot-45.png,","<p>The beetle had a lot of protein, which was noted during the processing.<\/p>\n<p>I assume that this fact may have something to due with the way that the bands appeared. They were very faded and some bands did not appear at all. Along with that, Ms. Lemon mentioned how the hard exoskeleton of the beetle made the gel electrophoreses process a tad bit difficult, so therefore that is more than likely another reason that the bands had trouble appearing.<\/p>\n",No,,,,
"",38.0001790000,-98.7445459000,08/14/2020,"<p>I found these Spotted Cucumber beetles on sunflowers. Before I preserved them in 70% ethanol they were a bright yellow and they blended in with the flowers really well. There were also Soldier beetles on the same sunflowers and blended in as well.<\/p>\n",Coleoptera,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/11/Wolbachia-Project-1.jpg,","<p>The beetle was stored in 90% ethanol for about a month before extraction.<\/p>\n<p>DNA Extraction: The DNA was extracted from the abdomen. All of the Dneasy (Qiagen) protocols were followed. The extraction took several days to complete.<\/p>\n<p>PCR: Mini One Taq polymerase was used.<\/p>\n<p>Gel Electropheresis: All of the lanes worked except for the DNA ladder. When pipetting, the well was missed and had to be done again which might have been the reason.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/8e.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/8d.jpg,",36.0497919174,-86.8985396502,08/30/2020,"<p>This milkweed bug is likely a nymph as it doesn&#8217;t look fully developed and was unable to fly. It was collected directly from a milkweed plant in an open field. A collection vial was quickly placed over top and immediately capped with a lid. The bug was then carefully tapped into a tube of 70% ethanol. There were also oleander aphids and small ants on the same plant.<\/p>\n",Oncopeltus fasciatus,DNeasy (Qiagen),Partial abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Screen-Shot-2020-09-20-at-8.08.20-PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Screen-Shot-2020-09-20-at-8.08.13-PM.png,","<p>The milkweed bug was stored in 70% ethanol for about 3 weeks prior to DNA extraction.<\/p>\n<p>DNA Extraction: DNA was extracted from very tip of abdomen (about 1\/3). The specimen was incubated in lysis buffer at 56C for 2 hours. Because there was cell debris, I did a 30 sec spin and transferred the supernatant to a fresh tube of 200ul ethanol. This was placed in the freezer overnight and purified the following day. Eluted DNA was immediately incubated at 65C for one hour prior to PCR.<\/p>\n<p>PCR: MiniOne Taq polymerase was used.<\/p>\n<p>Gel electrophoresis: The arthropod gel looks whispy; however, that went away as the gel ran longer. This could have been caused by not adding loading dye to samples. The results were very clear, though. I re-colored the gel images in PowerPoint. The MiniOne ladder contains 5 bands: 100, 300, 500, 1000, and 2000 bp.<\/p>\n",No,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/6h.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/6c.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/6d.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/6a.jpg,",36.0490913328,-86.8996113131,08/30/2020,"<p>This ladybird beetle was located on a milkweed plant. It was a bright sunny day and 80F.\u00a0 The beetle is presumably female as a male was trying to mate with her. She was a bright red color, but the pigmentation was diluted during ethanol preservation. She was collected directly from the milkweed plant by quickly placing a collection vial over top and immediately capping with a lid.<\/p>\n",Harmonia axyridis,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Screen-Shot-2020-09-20-at-8.05.50-PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Screen-Shot-2020-09-20-at-8.05.43-PM.png,","<p>The beetle was stored in 70% ethanol at room temperature for about 3 weeks prior to DNA extraction.<\/p>\n<p>DNA Extraction: DNA was extracted from the entire abdomen. It was likely too large because it created a thick slurry during lysis. The specimen was incubated in lysis buffer at 56C for 2 hours. Because there was cell debris, I did a 30 sec spin and transferred the supernatant to a fresh tube of 200ul ethanol. This was placed in the freezer overnight and DNA was purified the following day. Eluted DNA was immediately incubated at 65C for one hour prior to PCR.<\/p>\n<p>PCR: MiniOne Taq polymerase was used.<\/p>\n<p>Gel electrophoresis: The arthropod gel looks whispy; however, that went away as the gel ran longer. This could have been caused by not adding loading dye to samples. The results were very clear, though. I re-colored the gel images in PowerPoint. The MiniOne ladder contains 5 bands: 100, 300, 500, 1000, and 2000 bp.<\/p>\n",Yes,AGTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAGAGGAAGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTTTGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTTTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGATAAAACTCCTTTATTTGTATGATCTGTTCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTATTAACTGACCGAAACTTAAATTCTTCTTTTTTTGACCCAACCGGTGGGGGAGACCCAATTTTATACCAACATTT,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Ladybird-Arthropod_F.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/5c.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/5a.jpg,",36.0482371606,-86.9010671121,08/30/2020,"<p>I was walking through a wildflower park near Edwin Warner Park in Nashville, TN and this sweat bee landed on my arm. It was collected by quickly placing a collection vial over the bee and immediately capping with a lid. It was about 80F and very sunny.<\/p>\n",Lasioglossum,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Screen-Shot-2020-09-20-at-8.05.02-PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Screen-Shot-2020-09-20-at-8.04.55-PM.png,","<p>This sweat bee was stored in 70% ethanol for about 3 weeks prior to DNA extraction.<\/p>\n<p>DNA Extraction: The specimen was incubated in lysis buffer at 56C for 2 hours. Because there was cell debris, I did a 30 sec spin and transferred the supernatant to a fresh tube of 200ul ethanol. This was placed in the freezer overnight and DNA was purified the following day. Eluted DNA was immediately incubated at 65C for one hour prior to PCR.<\/p>\n<p>PCR: MiniOne Taq polymerase was used.<\/p>\n<p>Gel electrophoresis: The arthropod gel looks whispy; however, that went away as the gel ran longer. This could have been caused by not adding loading dye to samples. The results were very clear, though. I re-colored the gel images in PowerPoint. The MiniOne ladder contains 5 bands: 100, 300, 500, 1000, and 2000 bp.<\/p>\n",Yes,,,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/4c-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/4d-2.jpg,",36.1626638000,-86.7816016000,08/30/2020,"<p>This blowfly was buzzing around my patio, landed in my glass of water, and drowned. It was collected by using forceps to pull out of the water.<\/p>\n",Lucilia cuprina,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Screen-Shot-2020-09-20-at-8.02.10-PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Screen-Shot-2020-09-20-at-8.02.02-PM.png,","<p>The fly was stored in 70% ethanol at room temperature for about 3 weeks prior to DNA extraction.<\/p>\n<p>DNA Extraction: The fly was incubated in lysis buffer at 56C for 2 hours. Because there was cell debris, I did a 30 sec spin and transferred the supernatant to a fresh tube of 200ul ethanol. This was placed in the freezer overnight and DNA was purified the following day. Eluted DNA was immediately incubated at 65C for one hour prior to PCR.<\/p>\n<p>PCR: MiniOne Taq polymerase was used.<\/p>\n<p>Gel electrophoresis: The arthropod gel looks whispy; however, that went away as the gel ran longer. This could have been caused by not adding loading dye to samples. The results were very clear, though. I re-colored the gel images in PowerPoint. The MiniOne ladder contains 5 bands: 100, 300, 500, 1000, and 2000 bp. This sample was labeled as &#8220;blowfly&#8221;<\/p>\n",No,AATTGGAACTTCTTTAAGAATTCTAATTCGAGCTGAATTAGGACACCCTGGAGCTTTAATTGGAGATGATCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTAATGCCAATTATAATTGGAGGATTTGGAAATTGATTAGTTCCATTAATACTAGGAGCTCCAGATATAGCATTCCCTCGAATAAATAATATAAGTTTTTGACTTTTACCTCCTGCATTAACTTTATTATTAGTTAGTAGTATAGTAGAAAACGGAGCTGGAACAGGATGAACAGTTTACCCTCCCCTATCTTCTAATATTGCTCATGGAGGAGCTTCTGTTGATTTAGCTATTTTCTCTCTTCATTTAGCAGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACTACAGTTATTAATATACGATCAACAGGAATTACTTTTGATCGAATACCTTTATTTGTTTGATCAGTAGTAATTACAGCTTTATTGCTTTTATTATCATTACCAGTATTAGCAGGAGCTATTACAATACTTTTAACAGACCGAAATCTTAATACATCATTCTTTGATCCTGCAGGAGGAGGAGATCCAATTTTATACCAACATTTATTTT,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Blowfly-Arthropod_F.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/3d-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/3e.jpg,",36.0561223999,-86.9083882044,08/30/2020,"<p>A colony of black ants appeared to be living within the tree bark. One was collected at 11:50 am by placing a collection vial over top and allowing it to climb up.. The temperature was 78F, slightly cloudy, and 85% humidity. It is likely a carpenter ant.<\/p>\n",Camponotus pennsylvanicus,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Screen-Shot-2020-09-20-at-7.59.07-PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Screen-Shot-2020-09-20-at-7.59.00-PM.png,","<p>This sample was labeled as carpenter ant in the gel.<\/p>\n<p>The ant was stored in 70% ethanol for about 3 weeks prior to DNA extraction.<\/p>\n<p>DNA Extraction: The ant was incubated in lysis buffer at 56C for 2 hours. Because there was cell debris, I did a 30 sec spin and transferred the supernatant to a fresh tube of 200ul ethanol. This was placed in the freezer overnight and DNA was purified the following day. Eluted DNA was immediately incubated at 65C for one hour prior to PCR.<\/p>\n<p>PCR: MiniOne Taq polymerase was used.<\/p>\n<p>Gel electrophoresis: The arthropod gel looks whispy; however, that went away as the gel ran longer. This could have been caused by not adding loading dye to samples. The results were very clear, though. I re-colored the gel images in PowerPoint. The MiniOne ladder contains 5 bands: 100, 300, 500, 1000, and 2000 bp.<\/p>\n",Yes,AATTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTAGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTAATTCCACTTATACTAGGATCTCCTGATATAGCTTACCCTCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGAACTATCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTAGTATGATCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGCGCTATTACAATACTACTAACAGACCGAAATCTTAATACTTCATTTTTCGATCCCTCGGG,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/CarpenterAnt-Arthropod_F.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/2a-scaled.jpg,",36.0555258703,-86.9088502504,08/30/2020,"<p>This medium sized ant was crawling across a paved walking trail. It was moving so fast that I was unable to capture a live action picture. It was collected by placing a collection vial over top and allowing it to climb up. The ant has a black head, amber-colored thorax and legs, and a black abdomen. It was collected at 11:45am. 78F, 85% humidity, slightly cloudy.<\/p>\n",Camponotus chromaiodes,DNeasy (Qiagen),Abdomen,MiniOne,TBE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Screen-Shot-2020-09-20-at-7.55.17-PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Screen-Shot-2020-09-20-at-7.55.09-PM.png,","<p>The ant was stored in 70% ethanol at room temperature for about 3 weeks prior to DNA extraction.<\/p>\n<p>DNA Extraction: The ant was incubated in lysis buffer at 56C for 2 hours. Because there was cell debris, I did a 30 sec spin and transferred the supernatant to a fresh tube of 200ul ethanol. This was placed in the freezer overnight and DNA was purified the following day. Eluted DNA was immediately incubated at 65C for one hour prior to PCR.<\/p>\n<p>PCR: MiniOne Taq polymerase was used.<\/p>\n<p>Gel electrophoresis: The arthropod gel looks whispy; however, that went away as the gel ran longer. This could have been caused by not adding loading dye to samples. The results were very clear, though. I re-colored the gel images in PowerPoint. The MiniOne ladder contains 5 bands: 100, 300, 500, 1000, and 2000 bp.<\/p>\n",Yes,AGTTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTGGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTAATTCCCCTTATACTAGGATCTCCTGATATAGCTTACCCCCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGGACTGTCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTAGTATGGTCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGAGCTATTACAATACTACTAACAGACCGAAATCTTAATACTTCATTTTTCGACCCTTCGGGAGGGGGAGA,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Ant-Arthropod_F.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/1a-scaled.jpg,",36.0727326000,-86.9624086000,08/26/2020,"<p>Dozens of small ants were scurrying across the cement at a neighborhood swimming pool. One ant was collected at 3:15pm by placing a collection vial over top and allowing it to climb up. It was a cloudy day, 83F, 66% humidity.<\/p>\n",Lasius alienus,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Screen-Shot-2020-09-20-at-7.49.27-PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Screen-Shot-2020-09-20-at-7.49.18-PM.png,","<p>This sample is labeled as &#8220;small ant&#8221; on the above gels.<\/p>\n<p>The ant was stored in 70% ethanol at room temp for about 3 weeks prior to DNA extraction.<\/p>\n<p>DNA Extraction: The ant was incubated in lysis buffer at 56C for 2 hours. Because there was cell debris, I did a 30 sec spin and transferred the supernatant to a fresh tube of 200ul ethanol. This was placed in the freezer overnight and DNA was purified the following day. Eluted DNA was immediately incubated at 65C for one hour prior to PCR.<\/p>\n<p>PCR: MiniOne Taq polymerase was used.<\/p>\n<p>Gel electrophoresis: The arthropod gel looks whispy; however, that went away as the gel ran longer. This could have been caused by not adding loading dye to samples. The results were very clear, though. I re-colored the gel images in PowerPoint. The MiniOne ladder contains 5 bands: 100, 300, 500, 1000, and 2000 bp.<\/p>\n",No,TTGAGCTGGTATAATTGGCTCATCTATAAGAATAATTATCCGACTAGAATTAGGTTCATCTAATTCATTAATTAATAATGATCAAATTTATAACTCTATAGTTACAAGGCACGCATTTGTTATAATTTTCTTCATAGTTATACCTTTCATAATTGGTGGATTTGGTAATTTTCTTGTACCTTTAATATTAGGTTCACCTGATATGGCTTACCCCCGTATAAATAATATAAGATTTTGACTTTTACCTCCCTCTATTTCTCTACTCCTTTTAAGAAATTTCATTAATGATGGAGTCGGAACAGGATGAACCGTTTATCCTCCTTTAGCCTCAAATATCTTCCATAATGGCCCTTCAGTTGATTTAACTATTTTCTCTCTTCACATCGCTGGAATATCTTCTATTTTAGGAGCTATTAATTTTATTTCAACTATTATAAACATACACCATAAAAATTTTTCTATTGATAAAATTCCACTACTTGTATGATCAATCTTAATTACTGCAATTTTACTACTTCTATCCCTTCCTGTTCTTGCAGGAGCTATTACTATACTTTTAACTGACCGTAACCTTAATACTTCATTTTTTGACCCATCAGGAGGTGG,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/SmallAnt-Arthropod_F.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/7f.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/7h.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/7b.jpg,",36.0491315201,-86.8980705390,08/30/2020,"<p>These aphids were collected directly from a milkweed plant by scraping into a tube filled with 70% ethanol. It was a bright and sunny day, 80F. There were small ants and a milkweed bug on the same plant.<\/p>\n",Aphis nerii,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Screen-Shot-2020-09-20-at-7.37.32-PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Screen-Shot-2020-09-20-at-7.39.49-PM.png,","<p>The aphids were stored in 70% ethanol for about 3 weeks prior to DNA extraction. They were highly desiccated.<\/p>\n<p>DNA Extraction: DNA was extracted from four adults. They were incubated in lysis buffer at 56C for 2 hours. Following incubation, I did a 30 sec spin and transferred the supernatant to a fresh tube of 200ul ethanol. This was placed in the freezer overnight and purified the following day. Eluted DNA was immediately incubated at 65C for one hour prior to PCR.<\/p>\n<p>PCR: MiniOne Taq polymerase was used.<\/p>\n<p>Gel electrophoresis: The arthropod gel looks whispy; however, that went away as the gel ran longer. This could have been caused by not adding loading dye to samples. The results were very clear, though. I re-colored the gel images in PowerPoint. The MiniOne ladder contains 5 bands: 100, 300, 500, 1000, and 2000 bp.<\/p>\n",No,GGTATAATCGGATCTTCTCTTAGAATTTTAATTCGACTAGAATTAAGTCAAATTAATTCAATTATTAATAATAATCAATTATATAATGTAATTGTTACAATTCATGCTTTCATTATAATTTTTTTTATAACAATACCAATTGTAATTGGAGGATTTGGAAACTGGTTAATTCCTATAATAATAGGATGTCCAGATATATCTTTTCCACGATTAAATAATATTAGATTTTGATTATTACCTCCATCTTTAATAATAATAATTTGTAGTTTTATAATTAATAATGGAACAGGAACAGGATGAACAATTTATCCACCTTTATCAAATAACATTGCTCATAATAATATCTCAGTTGATTTAACTATTTTTTCTCTTCACTTAGCAGGAATTTCTTCTATTTTAGGAGCAATTAATTTTATTTGTACAATCCTAAACATAATACCTAATAATATAAAATTAAATCAAATTCCTCTATTTCCATGATCAATTTTAATTACAGCTATACTATTAATTTTATCTTTACCAGTATTAGCTGGTGCAATTACTATACTATTAACTGATCGAAATCTAAATACATCATTTTTTGACCCAGCAGGAGGGGGTGATCCTATCCTTTATCAACATTTATTTTGA,https://wolbachiaprojectdb.org/wp-content/uploads/2020/09/Aphid_706-Arthropod_F.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/06/tconfusum.jpeg,",33.9446470000,-84.3226488000,06/02/2020,"<p>Beetle samples from a culture from Josh&#8217;s Frogs herp site. trying to establish a working culture for further research.<\/p>\n<p>Gel Pic Below: Lanes 2-5 are T. confusum beetles. Lane 1 is positive W control and lane 9 negative W control.<\/p>\n",Insecta,,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/06/IMG_8705-1-scaled.jpeg,","<p>Wspec primers.<\/p>\n",Yes,,,,
"",47.3031293000,9.0880638000,05/30/2020,"<p>Sample 92<\/p>\n",Phaonia angelicae,,,,,,"","",Yes,TTTTTGGTGCTTGATCAGGAATAGTAGGAACTTCACTAAGTATTCTAATTCGAGCCGAATTAGGACACCCTGGAGCTTTAATTGGAGATGATCAAATTTATAATGTAATCGTAACAGCTCATGCTTTTGTTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGACTAGTGCCCCTAATATTAGGAGCCCCAGACATAGCCTTTCCTCGAATAAATAATATGAGATTTTGACTTTTACCTCCTGCTTTAACATTGCTGCTAACTAGCAGCATAGTAGAAACTGGAGCTGGAACAGGATGAACTGTTTACCCCCCACTATCATCTAATATTGCTCATGGAGGAGCCTCTGTTGATTTAGCTATTTTTTCCCTTCATTTAGCAGGAATCTCCTCTATTCTAGGAGCCGTAAATTTTATTACAACTGTAATTAATATACGATCAATAGGAATTACATTCGATCGCATACCTTTATTTGTATGATCAGTAGTAATTACAGCTTTATTATTACTTTTATCATTACCTGTTTTA,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/RA92-M13F.fasta,,
"",47.3031293000,9.0880638000,05/30/2020,"<p>Sample 90<\/p>\n",Phaonia angelicae,,,,,,"","",Yes,TTTTTGGTGCTTGATCAGGAATAGTAGGAACTTCACTAAGTATTCTAATTCGAGCCGAATTAGGACACCCTGGAGCTTTAATTGGAGATGATCAAATTTATAATGTAATCGTAACAGCTCATGCTTTTGTTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGACTAGTGCCCCTAATATTAGGAGCCCCAGACATAGCCTTTCCTCGAATAAATAATATGAGATTTTGACTTTTACCTCCTGCTTTAACATTGCTGCTAACTAGCAGCATAGTAGAAACTGGAGCTGGAACAGGATGAACTGTTTACCCCCCACTATCATCTAATATTGCTCATGGAGGAGCCTCTGTTGATTTAGCTATTTTTTCCCTTCATTTAGCAGGAATCTCCTCTATTCTAGGAGCCGTAAATTTTATTACAACTGTAATTAATATACGATCAATAGGAATTACATTCGATCGCATACCTTTATTTGTATGATCAGTAGTAATTACAGCTTTATTATTACTTTTATCATTACCTGTTTTAGCAGGAGCTATTACAATATTATTGACAGATCGAAATTTAAATACATCTTTTTTTGACCCTGCAGGAGGAGGAGATCCAGTATTATACCAGCATTTATTTTGATTTTTTG,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/RA90-M13F.fasta,,
"",47.3031293000,9.0880638000,01/09/2019,"<p>Sample 88<\/p>\n",Phaonia angelicae,,,,,,"","",Yes,TTTTTGGTGCTTGATCAGGAATAGTAGGAACTTCACTAAGTATTCTAATTCGAGCCGAATTAGGACACCCTGGAGCTTTAATTGGAGATGATCAAATTTATAATGTAATCGTAACAGCTCATGCTTTTGTTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGACTAGTGCCCCTAATATTAGGAGCCCCAGACATAGCCTTTCCTCGAATAAATAATATGAGATTTTGACTTTTACCTCCTGCTTTAACATTGCTGCTAACTAGCAGCATAGTAGAAACTGGAGCTGGAACAGGATGAACTGTTTACCCCCCACTATCATCTAATATTGCTCATGGAGGAGCCTCTGTTGATTTAGCTATTTTTTCCCTTCATTTAGCAGGAATCTCCTCTATTCTAGGAGCCGTAAATTTTATTACAACTGTAATTAATATACGATCAATAGGAATTACATTCGATCGCATACCTTTATTTGTATGATCAGTAGTAATTACAGCTTTATTATTACTTTTATCATTACCTGTTTTAGCAGGAGCTATTACAATATTATTGACAGATCGAAATTTAAATACATCTTT,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/RA88-M13F.fasta,,
"",,,,"<p>Sample 75<\/p>\n",Phaonia angelicae,,,,,,"","",Yes,TTTTTGGTGCTTGATCAGGAATAGTAGGAACTTCACTAAGTATTCTAATTCGAGCCGAATTAGGACACCCTGGAGCTTTAATTGGAGATGATCAAATTTATAATGTAATCGTAACAGCTCATGCTTTTGTTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGACTAGTGCCCCTAATATTAGGAGCCCCAGACATAGCCTTTCCTCGAATAAATAATATGAGATTTTGACTTTTACCTCCTGCTTTAACATTGCTGCTAACTAGCAGCATAGTAGAAACTGGAGCTGGAACAGGATGAACTGTTTACCCCCCACTATCATCTAATATTGCTCATGGAGGAGCCTCTGTTGATTTAGCTATTTTTTCCCTTCATTTAGCAGGAATCTCCTCTATTCTAGGAGCCGTAAATTTTATTACAACTGTAATTAATATACGATCAATAGGAATTACATTCGATCGCATACCTTTATTTGTATGATCAGTAGTAATTACAGCTTTATTATTACTTTTATCATTACCTGTTTTAGCAGGAGCTATTACAATATTATTGACAGATCGAAATTTAAATACATCTTTTTTTGACCCTGC,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/RA75-M13F.fasta,,
"",,,,"<p>Sample 85<\/p>\n",Phaonia angelicae,,,,,,"","",Yes,TTTTTGGTGCTTGATCAGGAATAGTAGGAACTTCACTAAGTATTCTAATTCGAGCCGAATTAGGACACCCTGGAGCTTTAATTGGAGATGATCAAATTTATAATGTAATCGTAACAGCTCATGCTTTTGTTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGACTAGTGCCCCTAATATTAGGAGCCCCAGACATAGCCTTTCCTCGAATAAATAATATGAGATTTTGACTTTTACCTCCTGCTTTAACATTGCTGCTAACTAGCAGCATAGTAGAAACTGGAGCTGGAACAGGATGAACTGTTTACCCCCCACTATCATCTAATATTGCTCATGGAGGAGCCTCTGTTGATTTAGCTATTTTTTCCCTTCATTTAGCAGGAATCTCCTCTATTCTAGGAGCCGTAAATTTTATTACAACTGTAATTAATATACGATCAATAGGAATTACATTCGATCGCATACCTTTATTTGTATGATCAGTAGTAATTACAGCTTTATTATTACTTTTATCATTACCTGTTTTAGCAGGAGCTATTACAATATTATTGACAGATCGAAATTTAAATACATCTTTTTTTGA,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/RA85-M13F.fasta,,
"",,,,"<p>Sample 84<\/p>\n",Culex pipiens,,,,,,"","",Yes,GTGTATTTATTGGAAATGATCAAATTTATAATGTTATTGTAACTGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCAATCATAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATGTTAGGAGCTCCAGATATGGCCTTTCCTCGAATAAATAATATAAGTTTTTGAATACTACCTCCTTCATTGACACTACTACTTTCAAGTAGTTTAGTAGAAAATGGGGCTGGGACTGGATGAACAGTGTATCCCCCTCTTTCATCTGGAACAGCTCATGCTGGAGCTTCAGTAGACTTAGCTATTTTTTCTTTACATTTAGCAGGAATTTCATCAATTTTAGGTGCAGTAAATTTTATTACAACAGTAATTAATATACGATCTTCAGGAATTACTCTTGATCGAATACCTTTATTTGTTTGATCAGTAGTAATTACTGCAGTTTTATTACTTCTTTCTTTACCTGTTTTA,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/RA84-M13F.fasta,,
"",,,,"<p>Sample 82<\/p>\n",Phaonia angelicae,,,,,,"","",Yes,TTTTTGGTGCTTGATCAGGAATAGTAGGAACTTCACTAAGTATTCTAATTCGAGCCGAATTAGGACACCCTGGAGCTTTAATTGGAGATGATCAAATTTATAATGTAATCGTAACAGCTCATGCTTTTGTTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGACTAGTGCCCCTAATATTAGGAGCCCCAGACATAGCCTTTCCTCGAATAAATAATATGAGATTTTGACTTTTACCTCCTGCTTTAACATTGCTGCTAACTAGCAGCATAGTAGAAACTGGAGCTGGAACAGGATGAACTGTTTACCCCCCACTATCATCTAATATTGCTCATGGAGGAGCCTCTGTTGATTTAGCTATTTTTTCCCTTCATTTAGCAGGAATCTCCTCTATTCTAGGAGCCGTAAATTTTATTACAACTGTAATTAATATACGATCAATAGGAATTACATTCGATCGCATACCTTTATTTGTATGATCAGTAGTAATTACAGCTTTATTATTACTTTTATCATTACCTGTTTTAGCAGGAGCTATTACAATATTATTGACAGATCGAAATTTAAATACATCTTTTTTTGACCCTGCAGGA,https://wolbachiaprojectdb.org/wp-content/uploads/2020/10/RA82-M13F.fasta,,
"https://wolbachiaprojectdb.org/wp-content/uploads/2020/05/image1-rotated.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2020/05/image0-rotated.jpeg,",36.1466598719,-86.8600582388,04/17/2020,"<p>I found this bumblebee outside the Nashville Humane Society.<\/p>\n",Hymenoptera,DNeasy (Qiagen),,MiniOne,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2020/05/IMG_7434-1-rotated.jpg,","<p>We used the kit sent to us by DMW, and the gel kit from MiniOne.<\/p>\n",,,,,