The arthropod was collected on the Benedictine University campus near the Quad area during the fall season. It was found resting on a milkweed leaf and was collected using a sweeping net. After capture, the insect was placed in a killing jar to secure and preserve the sample. The weather was sunny, and the collection occurred in the afternoon. The insect showed very little reaction when approached, remaining mostly still on the leaf in the open area.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

<\/div>\n

<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/article>\n

<\/div>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit.,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Gel-results.png,","",No,ACAGGTAAAGATAATAAAAGTAAAATTGCTCGTAATTAAAACAGATCATGTAAATAAAGGTAAAAAATTTAATGAATGGG TTTTAATATGTATATTTAAAATTGTTACAATAAAATTAATAGCTCCTATAATGGATGAAATTCCAGCAATATGAAGAGAA AAAATTGATAAATCAACAGAAGGTGAATTATGTCCAATATTTGAAGATAAAGGTGGGTATAAAGTTCATCCTGTTCCTAC TCCTATTTTTATTATGTTTCTTATAATAAGCAAAAATAATGAGGGTGGTAAAAGTCAAAATCTTATATTATTTATTCGTG GGAAAGCTATATCAGGGGCTCCAAGTATTATAGGAATTAATCAATTTCCAAAACCTCCAATTATAAATGGTATAACTATA AAAAAAATTATTACTAAAGCATGGGCTGTAATAATTGAATTATAAAATTGATCATTATTAATAATTGAAGATGGTGTTCC TAATTCTAATCGAATTATTAATCTTAAAGATGTACCAATTATTCCAGCTCAAAGTCCAAAAATAAAATATA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_0946-scaled.jpeg,",41.7787292009,-88.0959592204,09/01/2025,"

The insect is shaped like a leaf, which made it stand out from the background, it was found resting on the trunk of the tree.<\/p>\n",Fulgoridae,container,body without wings,agarose gel electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_1415-scaled.jpeg,","

In lane 3 is my Plant hopper(Fulgoroidea)\u00a0<\/em>sample. The gel showed a band for its DNA, indicating it was successful. It also showed a band for Wolbachia DNA , meaning my insect tested positive for Wolbachia.<\/p>\n",Yes,GATGTGCTGTAAATTTCGGTCTATAATTAATATTGTGATTGCTCCGGCTAGTACTGGTAAAGAAACTAGTAAAAGGATTG CTGTAATTAGTACTGATCAACAAAATAAGGGGGTTTTTTCTATTGTTATTCCTTTGATTCGTATATTTATAATGGTTGAA ATAAAATTAATTGCTCCTATAATTGATCTAATACCTGCAATGTGTAATGAAAAAATTGTTAAGTCTACTGATGGTCCTGA ATGTGCTTCTTGAGCCGAAAGAGGTGGGTATACAGTTCATCCTGTTCCTGTTCCTGAAGCTGCTGTTGATCTTGCAATTA ATAATGTAATTGATATTGGTAAAAGTCAAAAACTTATATTGTTTATTCGTGGGAAAGCTATATCTGGTGCTCCAATTATA AGAGGAACAAGCCAGTTTCCAAAACCACCAATTATAATTGGTATAACTATAAAAAAGATTATTACAAATGCATGTGAAGT TACAATTGTGTTATAAATTTGGTCATTTTTGATTATTGATCCTGGTTGAGATAGTTCTATTCGAATAATTATTCTTATTA TTATTCCTACTAGTCCTGATCAAATTCCAAATATAAAATATAGTGTTCCAATATCTTa,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/database-1-scaled.jpeg,",41.7762155911,-88.0965399236,09/02/2025,"

Collected by Fall 2025 students. Male mosquito with plumose (feathery) antennae. collected behind the Goodwin Hall building at the Benedictine University campus.attachments<\/a> $\"\"$ $\"\"$ $\"\"$ <\/p>\n",Anopheles gambiae,DNeasy (Qiagen) blood and tissue kit.,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/gel-image-scaled.jpg,","",No,ACAGTTGTAATTACTACTGATCAAACAAATAATGAAACTCCTGCCAGGTGTAAAGAAAAAATAGCCAAATCTACAGAAGC TCCTCTATGAGCAATTGCTGCTGATAAAGGAGGGTAAACAGTTCAACCTGTTCCTGCACCATTTTCAACAAATGAACTAG AAAGAAGAAGAGTAAGAGAAGGGGGTAAAAGTCAAAAACTTATATTATTTATTCGAGGAAATGCCATATCTGGGGCTCCT AATATTAAAGGAATTAATCAATTACCAAATCCTCCAATTAAAATAGGCATAACTATAAAAAAAATTATAATAAAAGCATG AGCTGTGACAACTACATTATAAATTTGATCATCTCCAATAAATGTACCAGGTCGTCCTAGTTCAGTTCGAATTAATATGC TTAGAGATGTACCAACTATCCCTGATCAAGCTTTGGGGGGGCGCACAAACGCCTAAATTCGGCCACTGC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/60374DAA-B192-4C47-866F-815248B913CE_1_105_c.jpeg,",41.7756400000,-88.0970000000,09/02/2025,"

I caught my Arthropod nead some tall grasses by a tree. This also happens to be the type of habitat in which\u00a0 my arthropod naturally resides.<\/p>\n",Opilio parietinus,DNeasy (Qiagen) blood and tissue kit.,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/28F80478-8139-4E2E-9D26-ED5323FC589C_1_201_a.jpeg,","

An error occurred during the step where the master mix was added to the DNA, which affected the samples before they were loaded onto the electrophoresis gel. Adding too much mastermix skewed the results and prevented any Arthropod DNA from showing in the results.I obtained a negative result for my arthropod sample, which was likely due to an error during preparation of the master mix<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_9399-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_9400-scaled.jpeg,",41.7756400000,-88.0970,12/08/2025,"

collected by Fall 2024 students.<\/p>\n",Anopheles,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-16.png,","

My numbers were within the minimum numbers.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_9565.jpg,",41.7784418000,-88.0952683000,09/02/2025,"

This arthropod specimen is a black fungus gnat that belongs to the genus Pseudolycoriella Sp<\/em>. It was collected outdoors at Benedictine University and is a small, dark colored fly commonly found in moist environments. The fungus gnat has thin long legs, one pair of wings, and a slender body, which are all characteristic features of dipteras.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-08-at-11.34.12 PM.png,","",No,>13-DA_G1_CoR.ab1 (524 bp) CAGTAATAAATACGGATCACACAAATAAAGGTATTTTATCAAATGTTATTCCTGGGGCTCGTATATTAATAATTGTTGAA ATAAAATTTACAGCCCCTAAAATTGATGAAATACCTGCAAGATGTAAGGAAAAGATTGAAAGATCTACAGAGGCCCCCGT ATGTGCAATAGTTGAAGACAAAGGAGGATAAACTGTTCAACCTGTTCCAGTTCCTCTATCTACCAAACTACTTGTTAATA AAAGAGTTAATGATGGAGGTAATAACCAAAATCTTATATTATTTAATCGGGGAAATGCTATGTCAGGGGCGGATAATATT AATGGAACTAATCAATTTCCAAATCCTCCAATTATAATTGGTATAACTATAAAAAAAATTATAATAAAAGCATGAGCAGT TACAATTACATTATAAATTTGATCATCTCCAATTAAAGCAAATGGATAACCTAATTCTGCTCGAATTAATATACTTAATG ATGTTCCTACTATTCTAGACCACGCCCCAAAAATAAAATATAAT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/My-Arthropod.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_6100-scaled.jpeg,",41.7756400000,-88.0970000000,09/04/2025,"

Collected by Fall 2025 students<\/p>\n

Captured using a beat net<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_6262-scaled-e1765255386456.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/tempImageh0QxBB-scaled.jpg,",41.7756400000,-88.0970000000,09/02/2025,"

The insect was collected within the Benedictine university’s area. It specifically was located near a lake that is close to the brick building. It was collected with a tweezer and container. Apparently, it was found on one of the large tree’s leaves near the brick lake.<\/p>\n",Harmonia axyridis,DNeasy (Qiagen) blood and tissue kit,body without wings,Standard electrophoresis system,TAE,SYBR Safe,"","",Yes,>39-FA_B2_CoR.ab1 (494 bp) GCCCCCCGGTGAAAAGATTTAAGTTTCGGTTAATATATAGTAATTGCTCCTGCAAGAACTGGAGATTAAAAGAATTGTGT AATAAGAACAGATCATACAAATAAAGGAGTTTTATCAAGATTATACCAAATGGACGTATATTTATAATATTGAAATGAAA TTTACTGCACCTAAAAGATTCGTGTTACGCCAGAATAACAGTCATCCTGTTCCTGCCCCTATTTCTACATTGTTCTTAAA ATTAAAAGAGTTAAAGCAGGGGGAAAGTCAAATCTTATGTTATTTAATCGGGAAATGCTATATCAGACTCCATTATTAAA GGAACTACATTTCCAAACCCCCAATTATAATAGTATTACTATAAAGAAAATTATAAGAATGACTGAACAATTATATTATA ATTTGGCGTTCCAATTACCTCTATCCTAATTCTAACAATTAATACTAACGATGTTCCACTATTCCTGCCCATATTCCAAA TAAAAATATAATGT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Maria-insect_Common-names-.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_2047-scaled.jpeg,",41.77564,-88.0970,09/02/2025,"

Collected off of tree near a lake<\/p>\n",Harmonia axyridis,DNeasy (Qiagen) blood and tissue kit.,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/40-FA_B3_CoR-CLEAN-MINE-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_7906-scaled.jpg,",41.7756400000,-88.0970000000,09/03/2025,"

This Damselfly was found in the tall grass around Lake Saint Benedict. We used the sweep net and swept it through the tall grass.<\/p>\n",Odonata,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Picture1.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/A9A26BD7-A5BC-4F72-B9EC-F7344D79C9E5.jpg,",41.77564,-88.0970,09/04/2025,"

Collected by Fall 2025 students.<\/p>\n

The field method used was a killing jar.<\/p>\n

The habitat in which the insect was caught was on a flowering plant.<\/p>\n",Bombus impatiens,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_7660-scaled.jpg,","",No,AATAAAGGAACACGGTCTATTCTAATTCCAGCTGCTCGTATATTAATTATAGTAGTAATAAAATTCACAGCCCCTAAAAT AGAAGAAGCTCCAGCAAGGTGGAGAGAAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/66-AA_V3_CoR-MY-DNA-SAMPLE.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_0945-scaled.jpg,",41.7756400000,-88.0970000000,09/02/2025,"

The insect has a distinctly bicolored body: the head and middle section (thorax) are reddish-brown, while the rear section (abdomen) is shiny and dark brown or black.<\/p>\n

<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_1241.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/5-DA_P1_CoR-6-USE-THIS-ONE.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/C348213A-1276-4FF7-A3C8-51B67C1084ED.jpeg,",41.7760375351,-88.0960307271,09/02/2025,"

The Roly-Poly was under a rock near the bushes. It was picked up with tweezers and it curled up when trying to pick it up to put in a container.<\/p>\n",Isopoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_0074.jpeg,","",No,GACCATTAAAGGATAAGGGGTCCCCCCCTCTCCGGGGGGATTATATAAATTAATTTTTATGTGATTAAAAATTGGCTTGG CGTGGAACAGGGAGAGAGATAAAATAAAAGATTGTTGTTACTACTGACCTACACTAAGGGTATTCGATTAAGTGAATTTC GTGGGGTGCGTATATTAATGACGGAGAAATAAAATTAACTACTGCTAAAATTGAAGAAGTGCCTGCTGGATGATGAGAAA AAATAGTTACATCTTCTGATGCCCCCCCATGAGCAATTACTGAAGAAAAAGGAGGATGATCAGCTCCTCCTGTTCCTCCT CCTTTTATACTATACTTGTTGCTAATATAAAAGATGGAGGAGGAGGTAAAACTCAAAATCTAATATTATTTATTTAAGCA AATGTTATATCTATGAGCACATATTAATAAAGGAACTAATTAATCCCCAAACCTACCAATTATTTTTGATATAACTTTAT AAAAATTTATAAGAAAAGAATGAGTGGGCTCAATTACTTTATTTATTTGATTATCCCCAACCAATGCTCCTGGATGTACT ACTTCAGTTCGAATTAATTAATATATAGATGTTTATACTATTTCAGCCCACGCTTATTACAATAAAATATAAAGTTTCTT ATTTTGTTTACC,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/38-FA_B1_CoR-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_5270-scaled.jpeg,",41.7756400000,-88.0970000000,,"

Collected by fall 2025 students<\/p>\n",Arthropoda,DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_5565-scaled.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_7505-scaled.jpeg,",41.7784418000,-88.0952683000,09/04/2025,"

Insect stayed still during time of collection<\/p>\n",Orthoptera,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",No,,,, "",41.7784418,-88.0952683,09/03/2025,"

Sweep net, found in shrubs<\/p>\n",Acanalonia conica,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/50-BW_G1_WoR.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_7534.jpeg,",41.7756400000,-88.0970000000,09/03/2025,"

Collected by Fall 2025 Students<\/p>\n

Common name:<\/b> Hairy caterpillar<\/span><\/p>\n

Order:<\/b> Lepidoptera<\/span><\/i> (moths and butterflies)<\/span><\/p>\n

Stage:<\/b> Larval form – actively feeding and growing<\/span><\/p>\n

Appearance:<\/b> Segmented body covered with protective hairs<\/span><\/p>\n

Behavior:<\/b> Slow-moving, uses hairs to deter predators<\/span><\/p>\n

Collection site:<\/b> Found on vegetation near campus, likely feeding on leaves<\/span><\/p>\n

Ecological role:<\/b> Herbivore, plant consumer, and important food source for birds and small animals<\/span><\/p>\n

\n
Segmented, cylindrical body covered with hairs<\/span><\/li>\n
Head:<\/b> chewing mouthparts<\/span><\/li>\n
Thorax:<\/b> 3 pairs of true legs<\/span><\/li>\n
Abdomen:<\/b> multiple pairs of prolegs with hooks<\/span><\/li>\n
Hairs (setae) used as a defense mechanism<\/span><\/li>\n
These features confirm <\/span>Order: Lepidoptera<\/b><\/li>\n<\/ul>\n

<\/p>\n",Lepidoptera,DNeasy (Qiagen) blood and tissue kit,Larva,Standard electrophoresis system,TAE,SYBR Safe,"","",Unknown,AAGCGTTGGTTAACCGGATCCCTCCTCCCGCAGGATCAAAAAAAGTAGTATTTAAATTACGATCAGTTAATAACATATGTAATAGCCCCCGCTAAGACAGGTAAGGATAATAATAATAAAACAGCAGTAATATTAACTGATCATCTAAATAAACATACTATTTCTTTTTTTACTCCTAATAAACAACAATTAATAATTGTTGAAATAAAATTTACAGCCCCCAAAATAGATGAAGCCCCCGCTAAATGTAAAGAAAAAATAGCCATATCAACTGAAATTCTCCTATGACCTAAAATTAAAGATAATGGAGGATAAACAGTCCACCCAGTACCAACCCCTCTTCCTAAAAACCCTCTCATTAACAATAAAAATACGGAAGGTACTAACAATCAAAATCTCATATTATTAAGACGAGGAAATGCTATATCAGCTGCCCCTAATATAAGAGGAACTAATCAATTACCAAATCCCCCAATTATAACAGGTATAACCATAAAAAAAATCATAACAAAAGCATGAGCCGTAACTAACCTATTATAAATTTGATCATTACCAATTACCACTCCAGGAGCCCCCAACTCCATACGAATTAATAATCTTATAGATAGCCCCAATATCCCAGCCCACAATCCAAACAAAAAATATAATATACCAATATCTTTATGATTTGTTGACCACA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/15-C_AY3_Co1_R-1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_5556-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_5559-1-scaled.jpeg,",41.7784418000,-88.0952683000,09/04/2025,"
\n
Collected Fall 2025<\/li>\n
Sweep net<\/li>\n
Found by the pond closest to our lab building (BK)<\/li>\n
I apprehended the insect while it was hopping mid-air through the grass and dirt<\/li>\n<\/ul>\n",Orthoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","
Limiting as much debrief and contamination as we can during DNA extraction and purification.<\/p>\n",Unknown,,,, "",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_0556-scaled.jpg,",41.7784418,-88.0952683,09/03/2025,"
Collected by Fall 2025 students.<\/p>\n",Scarites subterraneus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-123458-1.png,",41.778288068101375,-88.09553853195067,09/04/2025,"
The insect has a long, flattened brown body with noticeable pincer-like cerci at the end of the abdomen. It has short forewings, long antennae, and was found hiding under tree bark, which is a common habitat for earwigs.<\/p>\n",Forficula auricularia,container,Abdomen,agarose gel electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-06-110912.png,","
In Lane 2, which contained my European earwig (Forficula auricularia<\/em>) sample, the gel showed a clear insect DNA band, indicating that the DNA extraction and PCR amplification were successful. No Wolbachia band was present in this lane, showing that my specimen did not test positive for Wolbachia infection. These results demonstrate that my sample contained amplifiable insect DNA but lacked detectable Wolbachia DNA.<\/p>\n",No,ATTTAAGTTCCGGTCCGTCAAAAGCATAGTGATAGCCCCTGCCAATACTGGCAAAGAAAGCAACAATAAAAGAGCAGTAA TGGCTACGGATCATACAAACAACGGTATTCGTTCCAGCTTAAGGCCCGATGGGCGCATATTAATCACCGTTGTGATAAAA TTAATTGCCCCCAAAATTGATGAAATCCCTGCCAAATGCAATGAAAAAATACTCAAATCTACCGAAGCCCCTGCGTGGGC GATGGCCCCCGACAAAGGGGGGTAAACTGTCCAACCTGTACCAGCTCCTCTATCTACCATACTCCCAGAAAGCAAAAGCA TAAGCGAAGGGGGTAGTAACCAAAAACTCATGTTGTTTATTCGGGGAAAGGCCATATCTGGGGCTCTCAACATCAAAGGT ACCAGTCAATTCCCAAAACCTCCAATCATAATAGGCATTACCATAAAAAAAATCATTACAAAAGCATGGGCCGTAACAAT TACATTATAAATTTGATCATCTCCGATTAAAGCTCCAGGTTGGCCCAATTCTGCCCGAATCAACAAGCTCAATGAAGTCC CCACTATTCCTGATCAAGCCCCAAATACAAAATATAAAGTTCCAATATCTT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/myinsect-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_5018.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-11.28.31 AM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_4235.png,",41.7784418,-88.0952683,09/03/2025,"
Collected by Fall 2024 students.<\/p>\n",Melanoplus differentialis,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-03-205325.png,",41.7756400000,-88.0970000000,09/04/2025,"
collected by Fall 2025 students.<\/p>\n",Bombus terrestris,DNeasy (Qiagen) blood and tissue kit.,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-161549.png,","
Lab Manual<\/p>\n",No,>81-BA_G4_CoR (2).ab1 (381 bp) AAACTGTAAAGGAAAGACTAATGATCAAACAAATAAAGATTCCCTGTCGATATTAATTATAAAATTCCCTAAATAAAAAC TCCACAGGGGAAAAAATCTAATCTACAATGCCTCAATCAAGGCTGTCCTACTCCTCTTTCTTCTAAGAATAAAGTTAAAT ATTTATCTGGTCCTGAGATTATCCCCAATTATAATAGGTATTACTATAAAAAAAATTATTACAAAAGCATGAGCAGTTAC AATTACATTGTAAATTTGATCATCTCCAATTAAGCTTCCAGGTTGTCCTAATTCAGTGCGAATAATTATCCTAAGAGCAG TCCCAACAGCCCCTGCTCAAACCTCCAAATACAAAATATAAGGTCCCAATATCTTTGATTA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/81-BA_G4_CoR-2.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_2822.jpeg,",41.7767427158,-88.0958975803,09/02/2025,"
Collected by Fall 2025 students<\/p>\n
Sample collected by killing jar<\/p>\n
By the trees of the parking garage.<\/p>\n
<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_4650.jpg,","
I inserted my DNA in the third column….. and I have a strong band of visible DNA.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_4742-scaled.jpeg,",41.7773189699,-88.0968297699,09/04/2025,"
Collected by Fall 2025 students. Caught using a sweep net and a kill jar.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_3067-1-scaled.jpeg,","",No,>60-AA-Y1_CoR.ab1 (956 bp) AGCCCGGTGCGCGGAGAACGGTTGTTTCCAAAGGTAACCTCCCAATTTCGGGGGTTACAAACTCTCGGGG GGTGACTGGGGGGGGGTACAGGGCCCGGGAGATATTCACCGCGAATTGGTGATGCTCGATTATTAGAGAC TCCTACTACTTGAGGGAGAGGGGTAGCCGACAACGCACAATGATAAAAATTTTTTGAAAAAATTTCCTTC CCCCCGAGTCCCCGCTCCTTGTGGCACCCTGTGTGGCAGGTGTGGAGCCCGCCACGTAAGGAGCGGGATG ATGTGATGGTTCCTCGTTTCTTCCGGGGGGTTACCGGGGGGTTCTGTAAAGCCGCCCCTATATGATGGTT ACTAACAATAAAGGGGGGGGTCCTTCGGGGTTTACTTAACCAACTCCCTACCAAAACTAGCGACCAACAT CCTCCCCCTTGTTTCAATTTCCCGAAAGGGCTAACTTTTTTTTGAAATAGTCTCAGGATGTCAAGACCTG GTAAGGTTCTTCCCTTTACTTGAATTGAACCACATGCTCTACGCCGTGTGGGGGCCCCCCCCTTCTCTTT TTTTTTGATTTGGGCCCGGGGTCCCCCCCCCCAGAATTATATATTATATTTTTTGCTGCACGCTCAAAGG AACCAACCCCCCACCAACTACTGTTCATTTTGGGGGGCAGGAGCACCAAATCTTGTTGTTTCCTCTGCTC TCCCTCTCAGCCCAGCTCAGATACAGAGAAAAAAGACACATTCGCCATCGGCGGACTCCTCGTATCTACG CCTTTACAACAGGACACATGAGAATTCTCCTCTCCTCTCCCGTCATGCAAGTTAGAAGGGTTCTACAAGA AAGACCGGGCAATGTCAAGTTTAATAAAAATAAACGTATAAAGACGCTCGGGCCCCGTTACTCCCAAAAA AATCAACCCCCATATTAGGGGAGGGTTGGGGAAGGGTGCGGGGATG,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/60-AA-Y1_CoR.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_4423.jpg,",41.7756400000,-88.0970000000,09/02/2025,"
Collected by Fall 2025 students<\/p>\n
Caught by Maple Tree nearby the lake<\/p>\n",Forficula auricularia,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Image-12-5-25-at-11.24-AM.jpeg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/nc7-DA_Y1_CoR.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_0272.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_0271.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_0270-scaled.jpeg,",36.1265680362,-86.8288354088,09/02/2025,"
Collected by Fall 2025 students<\/p>\n",Megachilidae,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_0271-1.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_4621-scaled.jpeg,",41.7784418000,-88.0952683000,09/03/2025,"
Collected by fall 2025 section B Lab<\/p>\n",Melanoplus bivittatus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_0163.jpeg,","
$\"\"$ <\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-10.55.06 PM.png,",41.7784418,-88.0952683,09/04/2025,"
My specimen showed the typical characteristics of Bombus impatiens<\/em>: a black and yellow thorax, dense body hairs, large compound eyes, two pairs of membranous wings, and pollen baskets on the hind legs.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-11.08.59 PM.png,","
Two PCR attempts were performed. The first gel showed no bands possibly due to technical errors, and the second showed faint bands with no Wolbachia band present. Low 260\/230 ratio from the DNA extraction may have reduced PCR signal.<\/p>\n",No,TGATTAAAATTTCGATCAAAAAGATAAATTGCTCCGCTAAAACTGGTAATGATAAAATTAATAATAAAACTGTAATACAT ACAGATCAAAAAATAAAGTAATTTGATCATAATTTAATGAAAAATTTTTTATTAATATAATTGTAACAATAAAATTTAAT GAACCAATAATAGAAGAAATTCCTGTTATATGTAAAGAAAAAATTGCAATATCTACTGAGGGTGATGAATGAAATAAATA AGATGATAATGGAGGATATACAGTTCAACCTGTTCCTACATTTGGTGTAAATAGAGTTCTTAATAATAGTATAAAAATAG ATGGAGGTAATAATCAAAATCTAATATTATTTATTCGTGGGAAAGCTATATCAGGTGATCCTAATATTAAAGGAATTAAA TAATTTCCAAACCCTCCAATTATAAATGGTATAACTATAAAAAAAATTATTAAAAATGCATGTCTAGTAACTAATGAGTT ATAAATTTGATCATTATTAATTCATATTCCTGGATGTCTAAGTTCTATTCGAATTAATAATCTTATTGATGATCCAATTA TTCCTGATCATATGGCAAAAATAAAATATATTATTCATATCTTTTGA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Eastern_Bumblebee_COI.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_8716-scaled.jpeg,",41.7756400000,-88.0970000000,09/03/2025,"
Collected by fall 2025 students<\/p>\n",Melanoplus femurrubrum,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_0031.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_1101-scaled.jpeg,",41.77564,-88.0970,09/03/2025,"",Arthropoda,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Section-B-1.jpeg,","",No,,,, "",41.7760548757,-88.0962430959,09/02/2025,"
Collected by Fall 2025 students using swap net. Found in flowering bush plant low to the ground.<\/p>\n",Bombus impatiens,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/unnamed-2.jpg,",35.7289655000,-81.6860989000,09/25/2025,"
Caught on a bright and clear day in a pollen garden right outside the building where we have class. Caught by picking up with gloved hands and then putting into a bug net. Was caught around 1:00 PM, and was one of eight caught.<\/p>\n",Schistocerca americana,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/image_2025-12-04_214805089.png,","
DNA Extraction: I removed a majority of the insect that wasn’t its abdomen so that I could primarily focus on the innards found exclusively in the abdomen. I then took great care to crush it up thoroughly in a solution until I saw minimal\/no free-floating big clumps of debris.<\/p>\n
Lanes:<\/p>\n
\n
100bp Ladder<\/li>\n
Empty<\/li>\n
American Bird Grasshopper Sample 2 Arthropod Primers<\/li>\n
American Bird Grasshopper Sample 2 Wolbachia Primers<\/li>\n
Goldenrod Soldier Beetle Sample 3 Arthropod Primers<\/li>\n
Goldenrod Soldier Beetle Sample 3 Wolbachia Primers<\/li>\n
American Bird Grasshopper Sample 3 Arthropod Primers<\/li>\n
American Bird Grasshopper Sample 3 Wolbachia Primers<\/li>\n
Empty<\/li>\n
Arthropod Positive Control<\/li>\n
Arthropod Negative Control<\/li>\n
Wolbachia Positive Control<\/li>\n
Wolbachia Negative Control<\/li>\n<\/ol>\n
Analysis: My controls all worked, and while it’s faint, you can see bands for both the Arthropod DNA and the Wolbachia DNA, showing that Wolbachia can be present in Schistocerca americana.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_C5C2B51C-A6DD-4F97-9F05-89DAC5B08B35-scaled.jpeg,",41.7756400000,-88.0970000000,12/04/2025,"
collected by Fall 2025 students<\/p>\n",Nephrotoma,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/tempImageOIsk8V-scaled.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/images.jpeg,",41.7784418000,-88.0952683000,09/02/2025,"
It has<\/p>\n
Head<\/b>:\u00a0<\/span><\/p>\n
\n
Antennas\u00a0<\/span><\/li>\n
Mouthparts\u00a0<\/span><\/li>\n<\/ul>\n
Thorax<\/b>:\u00a0<\/span><\/p>\n
\n
Seven segments<\/span><\/li>\n<\/ul>\n
Abdomen<\/b>:<\/span><\/p>\n
\n
Six segments<\/span><\/li>\n
Breathing gills<\/span><\/li>\n<\/ul>\n
scaly back, mouth parts, 14 legs<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Section-B.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/tempImage69IHKP-scaled.jpg,",41.77564,-88.0970,09/04/2025,"
collected by Fall 2025 students<\/p>\n",Conocephalus brevipennis,DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-03-at-8.42.25-PM.png,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Arthropod-Sequence-.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_5156-scaled.jpg,",41.7766911613,-88.0954994141,09/03/2025,"
Collected by Fall 2024 students using sweep net, found in tall grass<\/p>\n",Blattella germanica,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/5AD05DAF-7AD8-4049-94DB-68B0080B7CC7.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/AA1129F9-1FD5-4B69-9EE0-FF0006144A70-2.jpg,",41.7756400000,-88.0970000000,09/03/2025,"
Collected by fall 2025 students, found under a rock near the lake.<\/p>\n",Armadillidium nasatum,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_8338.jpg,","",No,GTTTAAATTACGGCAGTCAAATAGCTCCCAACAGGAAAGATAAGAGAAAAAACCGCGGTTACTAAAACAGACCAAACAAA TAAAGGACCGATCCATACTAATTCCGGACGCCCGATATTAATTATAGGAATAAAGTTTACTGCCCCATTGATGAAGCCCC AAAGATGAAAGAAAAAACCTAAATCTACAGAAGCTCCACTATGGGCAATTCTTGATGCCAAAGGAGGGAAACAGTTCATC CCGTTCCACTCCTCTTTCAATTAATCCTCTGCTAAGGAGTAAAGTTAAAGAAGGGGGAAGCAGCCAAAATCTTATATTGT TTATAGAAAGCTATATCAGGAGCCCCTAATATCAAGGAATTAATCAATTTCCAAACCTCCATTATAATAGGATTACTATG AAAAAATCATTACAAAAGCATGACGGACATTACATTATAAGATCATCCAATGAGACTCCCAGTGCCCTAATTCCCGAATA ATTATTCTTAAAGGGACCACACTGCCAAACTCCGATATAAAATATAATGTACCAATATTTTATGAGTGCC,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Arthopod-84-BA_B3_CoR-1.fa,, "",35.7220916044,-81.6877467395,09/26/2025,"
Using mason jars, the kudzu bugs were caught from the kudzu patch near Western Piedmont Community College. A leaf was plucked from the kudzu, which contained a group of kudzu bugs on its backside. All the insects used in the experiment were caught on the same day, in the afternoon.<\/div>\n",Megacopta cribraria,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,MiniPCR,TBE,Cyber Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-7.36.48-PM.png,","
DNA Extraction: <\/b>The kudzu bug was first thoroughly washed with molecular biology-grade water, and then its abdomen was separated from the rest of the body using a sterile pair of tweezers, scalpel, and Petri dish. The reproductive organs were then transferred into a centrifuge tube containing ATL buffer and ground thoroughly with a pestle afterward.
\n
\nGel Electrophoresis Lanes:<\/b>
\nRed Box: <\/b>Sample Analyzed
\nLane 8: <\/b>Sample Amplified with Arthropod Primers
\nLane 9: <\/b>Sample Amplified with Wolbachia Primers
\nLane 10:\u00a0<\/b>Positive Wolbachia Control
\nLane 11:\u00a0<\/b>Negative Wolbachia Control
\nLane 12:\u00a0<\/b>Positive Arthropod Control
\nLane 13:\u00a0<\/b>Negative Arthropod Control<\/div>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/1000001518-e1764771446854.jpeg,",35.7296546215,-81.6856098322,09/25/2025,"
This grasshopper was found in the pollinator garden at NCSSM-Morganton. It was collected at around 1:00 PM using a bug net and identified with the i-Naturalist app.<\/p>\n",Schistocerca,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/ScreenShot-Tool-20251204104451.png,","
DNA Extraction:<\/b> I removed the majority of the arthropod, other than the abdomen, then took great care while crushing to make sure everything was broken up.\u00a0<\/span><\/p>\n
Gel Lanes:<\/b><\/p>\n
\n
100bp Ladder<\/span><\/li>\n
Empty<\/span><\/li>\n
American Bird Grasshopper Sample 2 Arthropod Primers<\/span><\/li>\n
American Bird Grasshopper Sample 2 Wolbachia Primers<\/span><\/li>\n
Goldenrod Soldier Beetle Sample 3 Arthropod Primers<\/span><\/li>\n
Goldenrod Soldier Beetle Sample 3 Wolbachia Primers<\/span><\/li>\n
American Bird Grasshopper Sample 3 Arthropod Primers<\/span><\/li>\n
American Bird Grasshopper Sample 3 Wolbachia Primers<\/span><\/li>\n
Empty<\/span><\/li>\n
Arthropod Positive Control<\/span><\/li>\n
Arthropod Negative Control<\/span><\/li>\n
Wolbachia Positive Control<\/span><\/li>\n
Wolbachia Negative Control<\/span><\/li>\n<\/ol>\n
Analysis:<\/b> The controls all worked, and although it was faint, there was a band present for both the arthropod and Wolbachia DNA from the sample.<\/span><\/p>\n",Yes,ATATATTTTTCCCGAAAATAAAAAATAAAGGTTTGAGATTACTACCGACCTCTCTTACCCTTCTTCTTACATCTTATAGAGTGACCCTG,,, "",35.7310842151,-81.6814910499,09/26/2025,"
Using mason jars, the kudzu bugs were caught from the kudzu patch near Western Piedmont Community College. A leaf was plucked from the kudzu, which contained a group of kudzu bugs on its backside. All the insects used in the experiment were caught on the same day, in the afternoon.<\/p>\n",Megacopta cribraria,Qiagen DNeasy Blood and Tissue Kit,Partial abdomen,MiniPCR,TBE,Cyber Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Kudzu.png,","
DNA Extraction:\u00a0<\/b>The kudzu bug was first thoroughly washed with molecular biology-grade water, and then its abdomen was separated from the rest of the body using a sterile pair of tweezers, scalpel, and Petri dish. The reproductive organs were then transferred into a centrifuge tube containing ATL buffer and ground thoroughly with a pestle afterward.
\n
\nGel Electrophoresis Lanes:<\/b>
\nRed Box: <\/b>Sample Analyzed
\nLane 4: <\/b>Sample Amplified with Arthropod Primers
\nLane 5: <\/b>Sample Amplified with Wolbachia Primers
\nLane 10:\u00a0<\/b>Positive Wolbachia Control
\nLane 11:\u00a0<\/b>Negative Wolbachia Control
\nLane 12:\u00a0<\/b>Positive Arthropod Control
\nLane 13:\u00a0<\/b>Negative Arthropod Control<\/p>\n",Yes,,,, "",35.7310967904,-81.6816420436,09/26/2025,"
Using mason jars, the kudzu bugs were caught from the kudzu patch near Western Piedmont Community College. A leaf was plucked from the kudzu, which contained a group of kudzu bugs on its backside. All the insects used in the experiment were caught on the same day, in the afternoon.<\/p>\n",Megacopta cribraria,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,MiniPCR,TBE,Cyber Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-7.18.45-PM.png,","
DNA Extraction: <\/b>The kudzu bug was first thoroughly washed with molecular biology-grade water, and then its abdomen was separated from the rest of the body using a sterile pair of tweezers, scalpel, and Petri dish. The reproductive organs were then transferred into a centrifuge tube containing ATL buffer and ground thoroughly with a pestle afterward.
\n
\nGel Electrophoresis Lanes:<\/b>
\nRed Box: <\/b>Sample Analyzed
\nLane 4: <\/b>Sample Amplified with Arthropod Primers
\nLane 5: <\/b>Sample Amplified with Wolbachia Primers
\nLane 6: <\/b>Positive Wolbachia Control
\nLane 7: <\/b>Negative Wolbachia Control
\nLane 8: <\/b>Positive Arthropod Control
\nLane 9: <\/b>Negative Arthropod Control<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Image_20251204_184912_877.jpeg,",35.7289601279,-81.6857797343,09/25/2025,"
$\"\"$ <\/p>\n
Using mason jars and a small mesh net, the Brown Marmorated Stink Bugs were caught from the windows of Goodwin Hall at the North Carolina School of Science and Mathematics – Morganton. All the insects used in the experiment were caught on the same day, around noon.<\/p>\n",Halyomorpha halys,Qiagen DNeasy Blood and Tissue Kit,Partial abdomen,MiniPCR,TBE,Cyber Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Alvin-.png,","
DNA Extraction:\u00a0<\/b>The brown marmorated stink bug was first thoroughly washed with molecular biology-grade water, and then its abdomen was separated from the rest of the body using a sterile pair of tweezers, scalpel, and Petri dish. The reproductive organs were then transferred into a centrifuge tube containing ATL buffer and ground thoroughly with a pestle afterward.
\n
\nGel Electrophoresis Lanes:<\/b>
\nRed Box: <\/b>Sample Analyzed
\nLane 2: <\/b>Sample Amplified with Arthropod Primers
\nLane 3: <\/b>Sample Amplified with Wolbachia Primers
\nLane 6: <\/b>Positive Wolbachia Control
\nLane 7: <\/b>Negative Wolbachia Control
\nLane 8: <\/b>Positive Arthropod Control
\nLane 9: <\/b>Negative Arthropod Control<\/p>\n",No,TAAACTTCAGGGTGACCAAAAAATCAAAATAAATGTTGATAAAGAATAGGGTCTCCTCCTCCTGATGGGTCAAAAAAAGAGGTATTAAAATTTCGGTCAGTTAATAGTATAGTAATAGCACCTGCTAACACAGGTAAGGATAATAACAGAAGAAGTGCAGTAATTCCTACTGATCACACAAACAATGGGATTCGTTCAGGGGTTATACCTGTTGGTCGTATATTTATAATAGTTGAAATAAAATTTACTGCCCCTAAAATTGATGAAACCCCAGCCAAATGTAAGGAAAAAATAGCTAAATCAACTGATGCTCCTCTATGTGAGATATTACTTGATAAGGGGGGATAGACTGTTCATCCTGTCCCAGCTCCTGATTCTGCTATTCTTCTTATTAATAATAAAGTTAATGAAGGGGGTAATAATCAGAATCTTATATTATTTAATCGTGGGAAGGCTATATCAGGTGCTCCAATTATTAAAGGGACTAATCAATTACCGAATCCTCCAATTATGATTGGTATTACTATAAAGAAAATTATTACAAATGCATGTGCTGTTACAATTACATTATAAATTTGATCATTACCAATAAATCTTCC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Image_20251204_184912_747.jpeg,",35.7305715908,-81.6862315981,09/25/2025,"
$\"\"$ <\/p>\n
Using mason jars and a small mesh net, the Brown Marmorated Stink Bugs were caught from the windows of Goodwin Hall at the North Carolina School of Science and Mathematics – Morganton. All the insects used in the experiment were caught on the same day, around noon.<\/p>\n",Halyomorpha halys,Qiagen DNeasy Blood and Tissue Kit,Partial abdomen,MiniPCR,TBE,Cyber Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Gel-3.png,","
<\/p>\n
DNA Extraction:\u00a0<\/b>The brown marmorated stink bug was first thoroughly washed with molecular biology-grade water, and then its abdomen was separated from the rest of the body using a sterile pair of tweezers, scalpel, and Petri dish. The reproductive organs were then transferred into a centrifuge tube containing ATL buffer and ground thoroughly with a pestle afterward.
\n
\nGel Electrophoresis Lanes:<\/b>
\nRed Box: <\/b>Sample Analyzed
\nLane 6: <\/b>Sample Amplified with Arthropod Primers
\nLane 7: <\/b>Sample Amplified with Wolbachia primers
\nLane 10:\u00a0<\/b>Positive Wolbachia Control
\nLane 11:\u00a0<\/b>Negative Wolbachia Control
\nLane 12:\u00a0<\/b>Positive Arthropod Control
\nLane 13:\u00a0<\/b>Negative Arthropod Control<\/p>\n",No,TAAACTTCCAGGGTGACCAAAAAATCAAAATAAATGTTGATAAAGAATAGGGTCTCCTCCTCCTGATGGGTCAAAAAAAGAGGTATTAAAATTTCGGTCAGTTAATAGTATAGTAATAGCACCTGCTAACACAGGTAAGGATAATAACAGAAGAAGTGCAGTAATTCCTACTGATCACACAAACAATGGGATTCGTTCAGGGGTTATACCTGTTGGTCGTATATTTATAATAGTTGAAATAAAATTTACTGCCCCTAAAATTGATGAAACCCCAGCCAAATGTAAGGAAAAAATAGCTAAATCAACTGATGCTCCTCTATGTGAGATATTACTTGATAAGGGGGGATAGACTGTTCATCCTGTCCCAGCTCCTGATTCTGCTATTCTTCTTATTAATAATAAAGTTAATGAAGGGGGTAATAATCAGAATCTTATATTATTTAATCGTGGGAAGGCTATATCAGGTGCTCCAATTATTAAAGGGACTAATCAATTACCGAATCCTCCAATTATGATTGGTATTACTATAAAGAAAATTATTACAAATGCATGTGCTGTTACAATTACATTATAAATTTGATCATTACCAATAAATCTTCCAGGCTGTCCTAATTCGATACGGATAATTAATCTTATAGCTG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/unnamed-2-1.jpg,",35.7305003289,-81.6862234567,09/25/2025,"
\n
Using mason jars and a small mesh net, the brown marmorated stink bugs were caught from the windows of Goodwin Hall at the North Carolina School of Science and Mathematics – Morganton. All the insects used in the experiment were caught on the same day, around noon.<\/span><\/p>\n
$\"\"$ <\/p>\n<\/div>\n",Halyomorpha halys,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,MiniPCR,TBE,Cyber Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-7.00.55-PM.png,","
DNA Extraction:\u00a0<\/b>The brown marmorated stink bug was first thoroughly washed with molecular biology-grade water, and then its abdomen was separated from the rest of the body using a sterile pair of tweezers, scalpel, and Petri dish. The reproductive organs were then transferred into a centrifuge tube containing ATL buffer and ground thoroughly with a pestle afterward.
\n
\nGel Electrophoresis Lanes:<\/b>
\nRed Box: <\/b>Sample Analyzed
\nLane 2: <\/b>Sample amplified with Arthropod Primers
\nLane 3: <\/b>Sample amplified with Wolbachia Primers
\nLane 10:\u00a0<\/b>Positive Wolbachia Control
\nLane 11:\u00a0<\/b>Negative Wolbachia Control
\nLane 12:\u00a0<\/b>Positive Arthropod Control
\nLane 13:\u00a0<\/b>Negative Arthropod Control<\/p>\n",No,CAGGGTGACCAAAAAATCAAAATAAATGTTGATAAAGAATAGGGTCTCCTCCTCCTGATGGGTCAAAAAAAGAGGTATTAAAATTTCGGTCAGTTAATAGTATAGTAATAGCACCTGCTAATACAGGTAAGGATAATAACAGAAGAAGTGCAGTAATTCCTACTGATCACACAAACAATGGGATTCGTTCAGGGGTTATACCTGTTGGTCGTATATTTATAATAGTTGAAATAAAATTTACTGCCCCTAAAATTGATGAAACCCCAGCCAAATGTAAGGAAAAAATAGCTAAATCAACTGATGCTCCTCTATGTGAGATATTACTCGATAAGGGAGGGTAGACTGTTCATCCTGTCCCAGCTCCTGATTCTGCTATTCTTCTTATTAATAATAAAGTTAATGAAGGGGGTAATAATCAGAATCTTATATTATTTAATCGTGGGAAGGCTATATCAGGTGCTCCAATTATTAAAGGGACTAATCAATTACCGAATCCTCCAATTATGATTGGTATTACTATAAAGAAAATTATTACAAATGCATGTGCTGTTACAATTACATTATAAATTTGATCATTACCAATAAATCTTCCAGGCTGTCCTAATTCGATACGGATAATTAATCTTATAGCTGATCCTACTA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/short-horned-grasshopper.jpeg,",41.7756400000,-88.0970000000,09/02/2025,"
Orthoptera found in grassy area by the campus quad<\/p>\n",Melanoplus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/gel-.jpeg,","",No,>4-CA_B4_CoR.ab1 (616 bp) GAnnTAnTTAAATTTCnATCAGTTAATAATATTGTAATAnCTCCTGCTAATACAGGAAGTGATAATAATAAAAGGAGTGC TGTAATAGCTACTGATCAAACAAATAATGGTGTTTGGTCTAGTGTTATTCTTTCTGATCGTATATTAATTGCTGTTGTAA TAAAATTGACTGCCCCTAGAATTGATGAAACACCAGCTAAGTGAAGAGAGAAAATGGCTAAATCAACTGACGCTCCACCA TGTGCAATTGCTCCAGCGAGTGGGGGGTAAACTGTTCATCCTGTACCAGCTCCATTATCAACCATAGATGAGGTAAGTAA AAGGGTTAGTGATGGTGGTAAAAGTCAAAAACTTATATTATTTATTCGTGGAAAAGCTATATCTGGTGCTCCAATTATTA ATGGTACAAGTCAATTACCAAATCCTCCAATTATAATAGGTATTACTATAAAGAAAATTATTACAAATGCGTGGGCTGTA ATAATTACATTATAGATTTGGTCATCTCCAATTAnAGATCCTGGTTGTCCTAGTTCTGCTCGAATAATTATTCTTATAGA AGTTCCTACTATTCCAGCTCATGCTCCAAATATAAAGTATAAGGTACCAATATCTT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Insect_CommonNames_with_Ayesha.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-4.25.17 PM.png,",35.72924242969324,-81.68711184803108,09/25/2025,"
Our sample was captured outside the Residence Hall of NCSSM-Morganton. It was found in the shrubs between the Residence Hall and Jeter.<\/p>\n",Harmonia axyridis,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,Cyber Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-4.34.26 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-4.35.16 PM-1.png,","
Standard protocol for processing the whole arthropod was followed\u2014no additional notes to report.<\/p>\n",No,GGGCAGGAATAGTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAGAGGAAGATTAAT TGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATA CCTATTATAATTGGGGGTTTTGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTC CACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTTTAATTTTAAGAACAATCGT AGAAATAGGGGCAGGAACAGGATGAACTGTTTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCT TCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCAGTAAATTTCA TTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGATAAAACTCCTTTATTTGTATGATCTGT TCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTATTAACT GACCGAAACTTAAATTCTTCTTTTTTTGACCCAACCGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-4.25.02 PM-1.png,",35.729200403110525,-81.68709989711441,09/25/2025,"
Our sample was captured outside the Residence Hall of NCSSM-Morganton. It was found in the shrubs between the Residence Hall and Jeter.<\/p>\n",Harmonia axyridis,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,Cyber Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-4.34.26 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-4.35.16 PM-1.png,","
Standard protocol for processing the whole arthropod was followed—no additional notes to report.<\/p>\n",No,GGAAGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCT TTATAGTAATACCTATTATAATTGGGGGTTTTGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGA TATAGCATTTCCACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTTTAATTTTA AGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTTTACCCTCCTCTTTCTTCTAATTTAACAC ATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGC AGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGATAAAACTCCTTTATTT GTATGATCTGTTCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTA TACTATTAACTGACCGAAACTTAAATTCTTCTTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Image_20251204_111504_228.jpeg,",35.7299747512,-81.6872624311,09/25/2025,"
The hill we needed to break had many clusters worth of eggs in it.<\/p>\n
<\/p>\n",Solenopsis invicta,DNeasy (Qiagen),eggs,MiniPCR,TBE,Cyber Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/gel-2.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/unnamed.png,","
Since these were eggs, we used two or three instead of just one to be positive we got some workable DNA.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Image_20251204_111522.png,",35.7299837320,-81.6872144340,09/25/2025,"
We collected these specimens from an “ant hill”, they swarmed the top of the newly broken hill, this was when we were able to collect them.<\/p>\n",Solenopsis invicta,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,Cyber Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/gel-2.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/unnamed.png,","
We did have contamination with our Arthropod negative control.<\/p>\n
For this sample I believe I used two ants instead of just one, since they were so small.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_5629-scaled.jpg,",35.73051750616508,-81.6859903046653,11/13/2025,"\n\n\n\n
After my teacher mentioned that her husband had been seeing unfamiliar insects in the window of his office, I checked the area and quickly noticed several boxelder bugs clustered near the frame. The temperature was around 55\u00b0F, which likely encouraged them to seek warmth indoors. I collected some of the bugs for closer observation.<\/td>\n<\/tr>\n
<\/th>\n<\/tr>\n<\/tbody>\n<\/table>\n",Boisea rubrolineata,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Untitled-presentation-5.jpg,","
DNA Extraction:<\/em> Because our boxelder bugs were smaller than a grain of rice, we did not dissect them. Instead, we used a standard whole-body tissue extraction method by placing a small portion of the bug into the extraction buffer and mechanically breaking down the tissue to release genomic DNA.<\/p>\n
Gel Electrophoresis:<\/em>\u00a0We amplified the extracted DNA using both arthropod and Wolbachia primers. The arthropod primer served as a positive control to confirm that the PCR reaction worked and that our DNA extraction was successful. The Wolbachia-specific primer allowed us to test for the presence of Wolbachia DNA within the boxelder bug samples. After PCR, we ran the products through gel electrophoresis to visualize amplification results and determine whether Wolbachia was present.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_5628-scaled.jpg,",35.73050861803268,-81.68603177320877,11/13/2025,"\n\n\n\n
After my teacher mentioned that her husband had been seeing unfamiliar insects in the window of his office, I checked the area and quickly noticed several boxelder bugs clustered near the frame. The temperature was around 55\u00b0F, which likely encouraged them to seek warmth indoors. I collected some of the bugs for closer observation.<\/td>\n<\/tr>\n
<\/th>\n<\/tr>\n<\/tbody>\n<\/table>\n",Boisea rubrolineata,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Untitled-presentation-4.jpg,","
DNA Extraction:<\/em> Because our boxelder bugs were smaller than a grain of rice, we did not dissect them. Instead, we used a standard whole-body tissue extraction method by placing a small portion of the bug into the extraction buffer and mechanically breaking down the tissue to release genomic DNA.<\/p>\n
Gel Electrophoresis:<\/em>\u00a0We amplified the extracted DNA using both arthropod and Wolbachia primers. The arthropod primer served as a positive control to confirm that the PCR reaction worked and that our DNA extraction was successful. The Wolbachia-specific primer allowed us to test for the presence of Wolbachia DNA within the boxelder bug samples. After PCR, we ran the products through gel electrophoresis to visualize amplification results and determine whether Wolbachia was present.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_5627-scaled.jpg,",35.730520774636624,-81.68599045593899,11/13/2025,"\n\n\n\n
After my teacher mentioned that her husband had been seeing unfamiliar insects in the window of his office, I checked the area and quickly noticed several boxelder bugs clustered near the frame. The temperature was around 55\u00b0F, which likely encouraged them to seek warmth indoors. I collected some of the bugs for closer observation.<\/td>\n<\/tr>\n
<\/th>\n<\/tr>\n<\/tbody>\n<\/table>\n",Boisea rubrolineata,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Untitled-presentation-3.jpg,","
DNA Extraction:<\/em> Because our boxelder bugs were smaller than a grain of rice, we did not dissect them. Instead, we used a standard whole-body tissue extraction method by placing a small portion of the bug into the extraction buffer and mechanically breaking down the tissue to release genomic DNA.<\/p>\n
Gel Electrophoresis:<\/em>\u00a0We amplified the extracted DNA using both arthropod and Wolbachia primers. The arthropod primer served as a positive control to confirm that the PCR reaction worked and that our DNA extraction was successful. The Wolbachia-specific primer allowed us to test for the presence of Wolbachia DNA within the boxelder bug samples. After PCR, we ran the products through gel electrophoresis to visualize amplification results and determine whether Wolbachia was present.<\/p>\n",No,GATCTGGTATAGTAGGTTCATCTTTAAGATGAATTATTCGTGTAGAATTAGGACAACCTGGTAGATTTAT TGGAGATGATCAAACATATAATGTAATTGTAACAGCACATGCTTTTATTATAATTTTCTTTATAGTTATG CCAATTATGATTGGTGGATTTGGGAATTGATTAGTGCCTTTAATAATTGGGGCCCCAGATATAGCATTTC CTCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCCCTTACTTTATTACTTGCAAGTAGTATAGT TGAAAGAGGGGCGGGAACTGGATGAACAGTTTACCCTCCTCTATCAAGAAATTTATCCCATAGAGGTGCC TCTGTAGATTTAGCAATTTTTTCATTACACTTAGCAGGTGTATCATCAATTTTAGGGGCTATTAATTTTA TTTCAACTATTATTAATATACGGCCAGCAGGAATAAGACCTGAACGAATACCATTATTTGTATGATCAGT AGGTATTACAGCCCTTCTGTTATTGTTGTCTTTACCTGTTTTAGCGGGGGCCATTACAATACTTTTAACT GACCGAAACTTTAATACATCTTTCTTTGATCCTACCGGAGGAG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_5626-scaled.jpg,",35.7285174643,-81.6904328565,11/13/2025,"
After my teacher mentioned that her husband had been seeing unfamiliar insects in the window of his office, I checked the area and quickly noticed several boxelder bugs clustered near the frame. The temperature was around 55\u00b0F, which likely encouraged them to seek warmth indoors. I collected some of the bugs for closer observation.<\/p>\n",Boisea rubrolineata,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Untitled-presentation-2.jpg,","
DNA Extraction:<\/em> Because our boxelder bugs were smaller than a grain of rice, we did not dissect them. Instead, we used a standard whole-body tissue extraction method by placing a small portion of the bug into the extraction buffer and mechanically breaking down the tissue to release genomic DNA.<\/p>\n
Gel Electrophoresis:<\/em>\u00a0We amplified the extracted DNA using both arthropod and Wolbachia primers. The arthropod primer served as a positive control to confirm that the PCR reaction worked and that our DNA extraction was successful. The Wolbachia-specific primer allowed us to test for the presence of Wolbachia DNA within the boxelder bug samples. After PCR, we ran the products through gel electrophoresis to visualize amplification results and determine whether Wolbachia was present.<\/p>\n",Yes,GATCTGGCATAGTAGGCTCATCTTTAAGATGAATTATTCGTGTAGAATTAGGGCAACCTGGTAGATTTAT TGGGGATGATCAAACATATAATGTAATTGTAACAGCACATGCTTTTATTATAATTTTCTTTATAGTTATG CCAATTATGATTGGTGGATTTGGGAATTGATTAGTGCCTTTAATAATTGGGGCCCCAGATATAGCATTTC CTCGAATAAATAATATAAGATTTTGACTTTTACCTCCTTCCCTTACTTTATTACTTGCAAGTAGTATAGT TGAAAGAGGGGCGGGAACTGGATGAACAGTTTATCCCCCTCTATCAAGAAATTTATCCCATAGAGGTGCC TCTGTAGATTTAGCAATTTTTTCATTACATTTAGCAGGTGTATCATCAATTTTAGGGGCTATTAATTTTA TTTCAACTATTATTAATATACGGCCAGCAGGAATAAGACCTGAACGAATACCATTATTTGTATGATCAGT AGGTATTACAGCTCTTCTATTATTGTTGTCTTTACCTGTTTTAGCGGGGGCCATTACAATACTTTTAACT GACCGAAACTTTAATACATCTTTCTTTGATCCTACCGGGGGAGGAG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_8071.png,",41.7756400000,-88.0970000000,09/03/2025,"
collected by Fall 2025 students<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit.,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_5625-scaled.jpg,",35.7305002111,-81.6860639008,11/13/2025,"
After my teacher mentioned that her husband had been seeing unfamiliar insects in the window of his office, I checked the area and quickly noticed several boxelder bugs clustered near the frame. The temperature was around 55\u00b0F, which likely encouraged them to seek warmth indoors. I collected some of the bugs for closer observation.<\/p>\n",Boisea rubrolineata,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Untitled-presentation-1.jpg,","
DNA Extraction:<\/em> Because our boxelder bugs were smaller than a grain of rice, we did not dissect them. Instead, we used a standard whole-body tissue extraction method by placing a small portion of the bug into the extraction buffer and mechanically breaking down the tissue to release genomic DNA.<\/p>\n
Gel Electrophoresis:<\/em> We amplified the extracted DNA using both arthropod and Wolbachia primers. The arthropod primer served as a positive control to confirm that the PCR reaction worked and that our DNA extraction was successful. The Wolbachia-specific primer allowed us to test for the presence of Wolbachia DNA within the boxelder bug samples. After PCR, we ran the products through gel electrophoresis to visualize amplification results and determine whether Wolbachia was present.<\/p>\n",Yes,GTAGGATCAAAGAAAGATGTATTAAAGTTTCGGTCAGTTAAAAGTATTGTAATGGCCCCCGCTAAAACAG GTAAAGACAACAATAATAGAAGAGCTGTAATACCTACTGATCATACAAATAATGGTATTCGTTCAGGTCT TATTCCTGCTGGTCGTATATTAATAATAGTTGAAATAAAATTAATAGCCCCTAAAATTGATGATACACCT GCTAAATGTAATGAAAAAATTGCTAAATCTACAGAGGCACCTCTATGGGATAAATTTCTTGATAGAGGGG GATAAACTGTTCATCCAGTTCCCGCTCCTCTTTCAACTATACTACTTGCAAGTAATAAAGTAAGGGAAGG AGGTAAAAGTCAAAATCTTATATTATTTATTCGAGGAAATGCTATATCTGGGGCCCCAATTATTAAAGGC ACTAATCAATTCCCAAATCCACCAATCATAATTGGCATAACTATAAAGAAAATTATAATAAAAGCATGTG CTGTTACAATTACATTATATGTTTGATCATCCCCAATAAATCTACCAGGTTGCCCTAATTCTACACGAAT AATTCATCTTAAAGATGAACCTACTATACCAGATC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Image-1.png,",35.72973957315658,-81.6869978963919,11/13/2025,"
My partner and I located this Woolly Worm near our school. It was on the road leading up to our school’s grass hill. It was crawling slowly across the road. I collected it in the afternoon about 11:30 AM, and it was cloudy and slightly windy.<\/p>\n",Pyrrharctia,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/tempImagePhikWn-scaled.jpg,","
DNA extraction: I crushed the arthropod thoroughly after dissecting it to just obtain its abdomen. I made sure to crush any hard parts.<\/p>\n
Gel electrophoresis lanes:<\/p>\n
\n
DNA Ladder<\/li>\n
second well is my arthropod DNA<\/li>\n
third well is my wolbachia DNA<\/li>\n
fourth well is my partners arthropod DNA<\/li>\n
fifth well is my partners wolbachia DNA<\/li>\n<\/ol>\n
Analysis: My bands were faint, yet present. They are enough to deduce a reasonable conclusion about the nature of our species, but the confidence level is low.<\/p>\n",Yes,GGGCAGGAATAGTAGGAACTTCATTAAGATTATTAATTCGAGCAGAATTAGGAATGCCCGGATCCTTAAT TGGAGATGATCAAATTTATAATACTATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATA CCTATTATAATTGGGGGATTTGGTAATTGATTAGTTCCTTTAATATTAGGAGCCCCTGATATAGCTTTCC CCCGAATAAATAACATAAGTTTTTGACTTTTACCCCCATCTTTAACTTTATTAATCTCAAGAAGAATTGT AGAAAATGGGGCAGGTACCGGATGAACCGTTTATCCCCCCCTTTCATCCAATATTGCCCACGGTGGTAGT TCTGTTGATTTAGCCATTTTTTCTTTACATCTAGCAGGAATTTCTTCAATTTTAGGAGCTATTAATTTTA TTACCACAATTATTAATATACGATTAAATAATTTATCATTTGACCAAATACCTTTATTTGTTTGAGCGGT AGGAATTACTGCTTTTTTACTTCTTCTTTCTTTACCTGTTTTAGCAGGTGCTATTACTATACTTTTAACA GATCGAAATTTAAATACATCATTTTTTGATCCTGCAGGGGGGGGAGATCCT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-11.26.26 AM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-11.26.29 AM.png,",35.7293514592,-81.6856477846,09/25/2025,"
Found crawling on the pavement.<\/p>\n",Apantesis phalerata,DNeasy (Qiagen),Partial abdomen,MiniPCR,TBE,SYBR Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-11.18.36 AM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-11.19.21 AM.png,","
I dissected the caterpillar, removing its head and upper half, and then its abdomen. I crushed the abdomen with a pestle, and that is what I ended up using for the amplification of the DNA.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-11.13.01 AM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-11.13.10 AM.png,",35.7295171862,-81.6863098104,09/25/2025,"",Harmonia axyridis,DNeasy (Qiagen),Partial abdomen,MiniPCR,TBE,SYBR Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-11.18.36 AM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-11.19.21 AM.png,","
I dissected the lady beetle, removing its wings and exoskeleton, and then its abdomen. I crushed the abdomen with a pestle, and that is what I ended up using for the amplification of the DNA.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_G1-scaled.jpg,",35.7294704020,-81.6855070200,09/26/2025,"
$\"\"$ <\/p>\n
This Goldenrod Soldier Beetle was found in a garden behind NCSSM in Morganton. It was collected around 1:00pm, it was one of 13 collected and identified with iNaturalist.<\/p>\n",Chauliognathus pensylvanicus,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/GSB-3-Gel.png,","
DNA Extraction:<\/strong> I removed the majority of the arthropod, other than the abdomen, then took great care while crushing to make sure everything was broken up.<\/p>\n
Gel Lanes:<\/strong><\/p>\n
\n
100bp Ladder<\/li>\n
Empty<\/li>\n
American Bird Grasshopper Sample 2 Arthropod Primers<\/li>\n
American Bird Grasshopper Sample 2 Wolbachia Primers<\/li>\n
Goldenrod Soldier Beetle Sample 3 Arthropod Primers<\/li>\n
Goldenrod Soldier Beetle Sample 3 Wolbachia Primers<\/li>\n
American Bird Grasshopper Sample 3 Arthropod Primers<\/li>\n
American Bird Grasshopper Sample 3 Wolbachia Primers<\/li>\n
Empty<\/li>\n
Arthropod Positive Control<\/li>\n
Arthropod Negative Control<\/li>\n
Wolbachia Positive Control<\/li>\n
Wolbachia Negative Control<\/li>\n<\/ol>\n
Analysis:<\/strong> My controls all worked, and while faint, I had a band for both the arthropod and Wolbachia DNA from the sample.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Image_20251204_102844_245-scaled-e1764862516413.jpeg,",35.7294704020,-81.6855070277,09/26/2025,"
This Goldenrod Soldier Beetle was found in a garden behind NCSSM in Morganton. It was collected around 1:00pm, it was one of 13 collected and identified with iNaturalist.<\/p>\n",Chauliognathus pensylvanicus,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/G1.png,","
DNA Extraction:<\/strong> I removed the majority of the arthropod, other than the abdomen, then took great care while crushing to make sure everything was broken up.<\/p>\n
Gel Lanes:<\/strong><\/p>\n
\n
100bp Ladder<\/li>\n
Empty<\/li>\n
Goldenrod Soldier Beetle Sample 1 Arthropod Primers<\/li>\n
Goldenrod Soldier Beetle Sample 1 Wolbachia Primers<\/li>\n
American Bird Grasshopper Sample 1 Arthropod Primers<\/li>\n
American Bird Grasshopper Sample 1 Wolbachia Primers<\/li>\n
Goldenrod Soldier Beetle Sample 1 Arthropod Primers<\/li>\n
Goldenrod Soldier Beetle Sample 1 Wolbachia Primers<\/li>\n
Empty<\/li>\n
Arthropod Positive Control<\/li>\n
Arthropod Negative Control<\/li>\n
Wolbachia Positive Control<\/li>\n
Wolbachia Negative Control<\/li>\n<\/ol>\n
Analysis:<\/strong> My controls all worked, and while faint, I had a band for both the arthropod and Wolbachia DNA from the sample.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_4762-scaled.jpeg,",35.7358452742,-81.8857217700,11/13/2025,"
My group partner and I found this Wolly worm on a sidewalk, near a forest-like place. We saw this Wolly worm in the morning, around 11 AM on a windy day. When we tried to collect the worm in a jar, we noticed that it kept compressing its body and folding up.<\/p>\n",Lepidoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Wolly-Worm-Gel.png,","
$\"\"$ DNA Extraction: Considering our Wolly Worm(s) were bigger than a grain of rice, we had to take the worm apart and pull out some DNA located right within the abdomen.<\/p>\n
Gel electrophoresis: We used both an arthropod and Wolbachia primer for amplification, as an arthropod primer helped us understand if the PCR is working properly and our DNA was extracted well. While, the Wolbachia primers helped us understand if there was a presence of such gene.<\/p>\n
1- Ladder<\/p>\n
3- Wolly Worm #1, Arthropod Primer<\/p>\n
5- Wolly Worm #1, Wolbachia Primer<\/p>\n
7- Wolly Worm #2, Arthropod Primer (My sample)<\/p>\n
9- Wolly Worm #2, Wolbachia Primer (My sample)<\/p>\n
(Our controls were conducted by two other group members, who were conducting the same protocols but for a Boxelder Bug (As our final study is the comparative study of Wolbachia presence between Lepidoptera’s (Wolly Worm in this case) and Hemipterans (Boxelder Bugs)<\/p>\n
Result Analysis: The Bands are not too bad, considering we can properly see the bp that was amplified by the Arthropod primers, and faintly see the bp amplified by Wolbachia primers.<\/p>\n",Yes,CAAAAAATGATGTATTTAAATTTCGATCTGTTAAAAGTATAGTAATAGCACCTGCTAAAACAGGTAAGGA AAGAAGAAGTAAAAAAGCAGTAATTCCCACTGCTCAAACAAATAAAGGTATTTGATCAAATGATAAATTA TTTAATCGTATATTAATAATTGTGGTAATAAAATTAATAGCTCCTAAAATTGAAGAAATTCCCGCTAGAT GTAAAGAAAAAATGGCTAAATCAACAGAACTACCACCGTGGGCAATATTGGATGAAAGGGGGGGATAAAC AGTTCATCCAGTACCTGCTCCATTTTCTACAATTCTTCTTGAGATTAATAAAGTTAAAGATGGGGGTAAA AGTCAAAAACTTATGTTATTTATTCGGGGGAAAGCTATATCAGGGGCTCCTAATATTAAAGGAACTAATC AATTACCAAATCCTCCAATTATAATAGGTATAACTATAAAAAAAATTATAATAAAAGCATGAGCTGTAAC AATAGTATTATAAATTTGATCATCTCCGATTAAGGATCCGGGTATCCCTAATTCTGCTCGAATTAATAAT CTTAATGAAGTTCCTACTATTCCTGCCCAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-10.34.13 AM.png,",35.7297728791,-81.6869860799,09/26/2025,"
We collected this arthropod by using a small net and jar to capture the bugs that were attracted to outside lights.<\/p>\n",Hypenodes caducus,pcr,Whole arthropod,MiniPCR,TBE,SYBR Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-10.57.36 AM.png,","",No,TAGGAACATCCCTCAGACTATTAATTCGAGCAGAATTAGGTACCCCTGGATCTTTAATTGGAGATGATCA AATTTATAATACTATTGTTACAGCACATGCTTTTATTATAATTTTTTTTATAGTAATACCAATTATAATT GGAGGATTTGGTAACTGATTAGTACCTCTTATATTAGGGGCCCCTGATATAGCATTTCCCCGAATAAATA ATATAAGTTTTTGATTATTACCCCCTTCTTTAACTCTTCTAATTTCAAGAAGTATCGTAGAAAATGGAGC AGGAACTGGGTGAACAGTTTATCCACCTTTATCATCTAATATTGCTCATGGGGGGAGATCAGTAGATTTA GCTATTTTCTCTCTTCATTTAGCTGGAATTTCATCAATTTTAGGAGCAATTAATTTTATTACTACAATTA TTAATATACGTCTTAATGGATTATCCTTTGATCAAATACCTTTATTTATTTGAGCTGTAGGAATTACAGC TTTTTTACTTCTTTTATCCCTACCTGTATTAGCTGGAGCAATTACAATATTATTAACTGATCGAAATCTT AATACTTCTTTTTTCGATCCTGCTGGAGGAGGAGAC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-10.47.12 AM.png,",35.7305306000,-81.6862142000,09/26/2025,"",Draeculacephala robinsoni,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-10.55.08 AM.png,","",No,TAATAGCACCAGCTAAAACTGGTAGTGATAATAATAATAAAATTGCAGTAATTATAACTGATCATGCAAA TAGTGGTGTTCGATCTAAATTTATTCCAATTCTTCGTATATTAATTAGTGTTGTAATAAAATTTACTGCT CCTAAAATTGATGAAATTCCAGCTAAATGTAATGAGAAAATAGCTAGATCAACTCTAGGTCCTGAGTGTG CTGTATTAGATGATAGCGGTGGATATACCGTTCAACCGGTTCCTGCTCCTATTTCTACTATAGATCTTAC TAATAGTAGTGTAAGTGATGGGGGTAAAAGTCAAAATCTTATATTATTTATTCGCGGAAATGCTATATCT GGTGCTCCAATTATTAATGGTAATAATCAATTACCGAATCCTCCAATTATAATAGGTATTACTATAAAAA AAATTATAATAAATGCATGTGATGTTACAATTACATTATATAACTGATCATTTGCTAAGAATGGTC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-10.26.38 AM.png,",35.7305306000,-81.6862142000,09/26/2025,"
Caught with UV light behind sheet.<\/p>\n",Staphylinidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-10.58.25 AM.png,","",No,TTAATATTCTTGGTGATAATTACCAATTATATAATGTAATAGTTACAAGTCATGCTTTGGTTATGGTATT TTTTATGATTATGCCAGCTCTAATGGGTGGCTTTGGTAATTGGTTTGTTCCTCTTATGATAGGTGCACCG GATATGGCTTTTCCGCGTATGAATAATTTGAGCTTTTGGCTACTGATATCATCCTTTGTCTTGCTCATTA TGTCTCTGTTTATTGGCGAAGGAGCAGGTACTGGTTGGACGTTATACCCACCACTATCACAAGTTAATTC CCATCCTAGTGCAGCTGTTGATCTGACAATATTTGCACTTCATGTTGCTGGGGTCTCCTCAATTGTTGGT GCTATTAATTTTATCGTTACTATATTTAATATGCGCACTCCTGGGATGACCTTGCATAAGATGCCTTTAT TTGTTTGGTCTATTTTACTTACCGCTTTTATGCTCATAGTTGCTTTGCCAGTATTAGCTGGAGCTATCAC TA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/11EE6004-914D-4751-B0F4-21DA8F498951-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/8364D9BF-75B3-4202-96FB-1A5E81A555B9.jpeg,",35.7297466754,-81.6869440122,09/26/2025,"
We set up a sheet with an led light behind it, left it for an hour then folded the sheet up and used a jar to capture the bugs.<\/p>\n",Diabrotica undecimpunctata,pcr,Whole arthropod,MiniPCR,TBE,Cyber Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-10.52.05-AM.png,","",No,GGTCAACAAAATCATAAAGATATTGGAACATTATATTTCATTTTTGGAATTTGAGCAGGTATAATTGGAACATCCCTAAGAATTCTAATTCGAGCAGAATTAGGTAGACCCGGTTCCTTAATTGGTAATGATCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCTATTATAATTGGCGGATTTGGTAACTGACTAGTCCCATTAATAATTGGAGCCCCTGATATAGCATTCCCTCGAATAAATAATATAAGATTTTGACTTTTACCACCCTCTTTATTATTATTATTATTAAGAAGAATAGTAGAAAATGGAGTTGGTACCGGATGAACAGTTTATCCCCCTTTATCATCCAATATTGCTCACGGAGGTTCATCAGTTGATTTAGCTATTTTTAGTTTACACTTGGCTGGAATTTCTTCAATTTTAGGAGCAATTAATTTTATTACTACAGTAATTAATATACGTCCAATAGGAATAACTTTTGACCGAATACCATTATTTGTATGAGCAGTTGTTATTACAGCAGTTTTACTACTATTATCATTACCTGTTCTTGCCGGGGCAATTACAATATTATTAACAGATCGTAATCTAAATACCTCTTTTTTTGATCCTGCTGGAGGTGGTGATCC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/ob.png,",35.7306418789,-81.6862140688,09/26/2025,"
Passive Phototactic Sampling using j<\/span>ar collection under existing light. <\/span>Used existing lights on the outside of our Barn to attract insects. <\/span>Collected samples using a jar and then s<\/span>oaked arthropods in ethanol.<\/span><\/p>\n",Olethreutes baccatana,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/GelElectro.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/gel-1.png,","",No,AGTTGGAACTTCCTTAAGATTATTAATTCGAGCTGAATTAGGAAATCCAGGATCATTAATTGGAGATGATCAAATTTATAACACTATTGTTACTGCTCATGCCTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGTAATTGATTAGTACCTTTAATATTAGGAGCTCCTGATATAGCTTTCCCACGAATAAATAATATAAGATTTTGATTATTACCCCCCTCTCTTATATTATTAATTTCAAGAAGAATTGTAGAAAATGGAGCAGGAACAGGATGAACAGTTTACCCCCCACTTTCATCAAATATTGCTCACAGAGGAAGATCAGTAGATCTTGCTATTTTTTCTCTACATTTAGCTGGAATTTCATCAATTTTAGGAGCTGTTAATTTTATTACAACTATTATTAATATACGACCTAATAATATATCTTTAGATCAAATACCTTTATTTGTTTGATCAGTAGGAATTACAGCTTTATTATTACTTCTTTCTTTACCAGTATTAGCAGGAGCCATTACAATATTATTAACTGATCGAAATCTTAATACATCTTTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3965-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3964-scaled.jpeg,",38.9420448555,-77.0895239679,09/15/2025,"
Caught the arthropod by the base of a tree in a park. Caught it nearing the first day of fall in the end of the summer. The temperature when I caught it was 74* F in a terrestrial habitat. The arthropod was some kind of ant with antenna, and it was mainly black with some brown.<\/p>\n",Hymenoptera,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0030.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0031.jpeg,","
DNA Extraction:<\/p>\n
Arthropod was not hard to extract DNA from. Crushed easily. No mistakes recorded.<\/p>\n
PCR:<\/p>\n
Had to redo the PCR because the first one didn\u2019t work. Worked the second time around.<\/p>\n
Gel electrophoresis, Arthropod CO1:<\/p>\n
In the arthropod gel we found a band signaling that B-4 was indeed an arthropod. In the B1-4 lanes of the gel electrophoresis, we had the corresponding DNA for each arthropod. In the W+ lane was a Wolbachia positive control and in the W- lane was a Wolbachia negative control, and in the H20 lane was a water control. We also found bands for arthropods B1-3 and our W+ and W- controls. Our controls worked meaning that we are confident in the results. We had no mistakes in this process such as contamination.<\/p>\n
Gel electrophoresis, Wolbachia:<\/p>\n
There was a band for Wolbachia in the B-4 lane of our gel. In the B1-4 lanes of the gel electrophoresis, we had the corresponding DNA for each arthropod. In the W+ lane was a Wolbachia positive control and in the W- lane was a Wolbachia negative control, and in the H20 lane was a water control. We also found bands for B3 and our W+ control. There was no band for arthropods B1-2 and our W- control. Our controls worked as intended meaning that we can confidently say that B-4 was infected with Wolbachia.<\/p>\n",Yes,AATTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTAGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCA ATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTT TTAATTCCACTTATACTAGGATCTCCTGATATAGCTTACCCTCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATC GATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGAACTATCTACCCCCCTCTATCATCAA ATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCA ATCAATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTAGTATGATCTAT TCTTATTACAGCTATTCTCCTTCTTCTATCCCTACCTGTTCTAGCAGGCGCTATTACAATACTACTAACAGACCGAAATC TTAATACTTCATTTTTCGATCCCTCGGGAGGAGGAGATCCTATTTTATACCAACATTTATTTTGA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/B4CO1-CO1F_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0046-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0045-scaled.jpeg,",38.9422290475,-77.0890261129,09/15/2025,"
Found underneath a rock outside. Looks to have 50+ legs. I believe it is a millipede or a centipede. Appears to have 2 antennas. Brown, black, dark tan, and shiny. Eyes not visible. Found in terrestrial habitat and caught when the temperature was 74* F.<\/p>\n",Diplopoda,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"","
DNA Extraction:<\/p>\n
Arthropod was easy to extract DNA from. Crushed easily. No mistakes recorded.<\/p>\n
PCR:<\/p>\n
We ran the PCR once and then we ran it again because we couldn\u2019t see any bands for our arthropod gel electrophoresis. The second time the PCR went as planned. We did not identify any signs of contamination.<\/p>\n
Gel electrophoresis, Arthropod CO1:<\/p>\n
In the arthropod gel we did not find a band under the B-5 lane. In the B-5 and B-6 lanes of the gel electrophoresis, we had the corresponding DNA for each arthropod. In the W+ lane was a Wolbachia positive control and in the W- lane was a Wolbachia negative control, and in the H20 lane was a water control. We found bands for arthropod B-6 and our W+ and W- controls. Our controls worked meaning that something went wrong for this arthropod specifically. We cannot be certain about any results from this arthropod because there was not enough DNA to confirm that it was an arthropod.<\/p>\n
Gel electrophoresis, Wolbachia:<\/p>\n
There was not a band for Wolbachia in the B- 5 lane of our gel. In the B-5 and B-6 lanes of the gel electrophoresis, we had the corresponding DNA for each arthropod. In the W+ lane was a Wolbachia positive control and in the W- lane was a Wolbachia negative control, and in the H20 lane was a water control. We found a band for our W+ control. There was no band for arthropods B-6 and our W- control. Our controls worked as intended but there was not sufficient DNA for the arthropod gel so we cannot conclude if there was Wolbachia in our B-5 arthropod or not. We did not send the DNA to the lab for testing because there was no band present for the arthropod gel meaning it did not have enough DNA to get results from the lab.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0044-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0047-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0043-scaled.jpeg,",38.9422006117,-77.0890563808,09/15/2025,"
Found this arthropod underneath a rock with near a couple other arthropods. Caught it nearing the first day of fall in the end of the summer. The temperature when I caught it was 74* F in a terrestrial habitat. The arthropod had more than 8 legs and appeared to be a pill bug. It was gray and brownish gray.<\/p>\n",Armadillidium,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"","
DNA Extraction:<\/p>\n
Arthropod was easy to extract DNA from. Crushed easily. No mistakes recorded.<\/p>\n
PCR:<\/p>\n
We ran the PCR once and then we ran it again because we couldn\u2019t see any bands for our arthropod gel electrophoresis. The second time the PCR went as planned. We did not identify any signs of contamination.<\/p>\n
Gel electrophoresis, Arthropod CO1:<\/p>\n
In the arthropod gel we found a band under the B-6 lane. In the B-5 and B-6 lanes of the gel electrophoresis, we had the corresponding DNA for each arthropod. In the W+ lane was a Wolbachia positive control and in the W- lane was a Wolbachia negative control, and in the H20 lane was a water control. We found bands for our W+ and W- controls. There was not band under the B-5 arthropod. Our controls worked meaning that something went wrong for B-5 arthropod specifically. We cannot be certain about any results from the B-5 arthropod, but we can trust the results from the B-6 arthropod.<\/p>\n
Gel electrophoresis, Wolbachia:<\/p>\n
There was not a band for Wolbachia in the B-6 lane of our gel. We found a band for our W+ control. There was no band for arthropods B-5 and our W- control. Our controls worked as intended so we can conclude that the B-6 was Wolbachia negative.<\/p>\n",No,AATAATTATTCGGACTGAATTnnnNNnnnnnnnGAGTCTCATTGGGGATGATCAAATCTATAATGTAnnnnnnnCGGCTC ATGCTTTTGTAATGATTTTCTTCATAGTAATACCTATTATAATTGGAGGTTTTGGAAATTGATTAATTCCTTTGATATTA GGGGCTCCTGATATAGCTTTCCCTCGTATAAACAATATAAGATTTTGGCTGCTTCCCCCTTCTTTAACTTTACTCCTTAG CAGAGGATTAATTGAAAGAGGAGTAGGAACGGGATGAACTGTTTACCCTCCTTTGGCATCAAGAATTGCCCATAGTGGAG CTTCTGTAGATTTAGGTATCTTTTCTCTTCATCTTGCTGGGGCTTCATCAATTTTAGGGGCAGTAAACTTTATTACGACT ATAATTAATATACGGGCGTCCGGAATTAGTATGGATCGTGTGCCTTTATTTGTTTGGTCTGTTTTAGTAACCGCGGTTCT TCTTCTCTTATCTTTACCTGTTCTTGCTGGAGCTATTACTATACTATTGACTGACCGTAATTTAAATACATCTTTTTTTG ATCCGAGGGGAGGGGGGGATCCTATTCTTTATCAGCATTTATTCTGATTTTTTG,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/B6CO1-CO1F_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_9415-scaled.jpeg,",41.7784418000,-88.0952683000,09/04/2025,"
I used a aspirator to capture my insect which was found in the walk path near grass outside of Goodwin.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_0192-scaled-e1764813358959.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-03-160415.png,",45.55498997557451,-122.64578370790406,10/08/2025,"",Nebria brevicollis,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,1X TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Thursday-Gel-2025-1.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/EG_GreenbottleFly-scaled.jpeg,",45.4869283,-122.804032,10/21/2025,"",Lucilia sericata,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,1X TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Thursday-Gel-2025-1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/JF_TribeEmpoascini_1.jpg,",45.45302727197288,-122.82596767326355,10/16/2025,"",Cicadellidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,1X TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Thursday-Gel-2025-1.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/AF_WesternYellowjacket_3-scaled.jpg,",45.5124935067,-122.6868900681,,"",Vespula pensylvanica,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,1X TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Thursday-Gel-2025-1.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/JFL_centipede_1.jpg,",45.50933905465824,-122.67268768570557,10/15/2025,"",Chilopoda,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,1X TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Thursday-Gel-2025-1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-03-141725.png,",45.474021353784465,-122.70273050527955,10/14/2025,"",Leptoglossus occidentalis,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,1X TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Thursday-Gel-2025.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/MN_Weevil_4-scaled.jpg,",45.47285753473271,-122.50568054813232,10/09/2025,"",Curculionidae,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,1X TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Tuesday-Gel-2025-1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/LT_BrownCentipede_2-scaled.jpeg,",45.53397127962525,-122.68434829311524,10/12/2025,"",Lithobiomorpha,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,1X TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Tuesday-Gel-2025-1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/NIU-210-scaled.jpg,",41.9295355,-88.74991279999999,07/21/2025,"
spider<\/p>\n",Araneae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/NIU-209-1-scaled.jpg,",41.9295355,-88.74991279999999,07/21/2025,"
beetle<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/CD_BrownMarmoratedStinkBug_1.jpg,",45.5145334,-122.6840848,10/10/2025,"",Halyomorpha halys,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,1X TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Tuesday-Gel-2025-1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/AT_PlodiaInterpunctella5-scaled.jpg,",45.515232,-122.6783853,10/07/2025,"",Pyralidae,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,1X TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Tuesday-Gel-2025-1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/LG_Pelecomalium-testaceum_2-scaled.jpg,",45.515232,-122.6783853,10/12/2025,"",Staphylinidae,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,1X TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Tuesday-Gel-2025.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/NIU-209-scaled.jpg,",41.9295355,-88.74991279999999,07/21/2025,"
beetle<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/NIU208-scaled.jpg,",41.9295355,-88.74991279999999,07/21/2025,"
thrips<\/p>\n",Thysanoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "",41.9295355,-88.74991279999999,07/21/2025,"
bee<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/NIU-206-scaled.jpg,",41.9295355,-88.74991279999999,07/21/2025,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/NIU-205-scaled.jpg,",41.9295355,-88.74991279999999,07/21/2025,"
moth<\/p>\n",Lepidoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/NIU-204-scaled.jpg,",41.9295355,-88.74991279999999,07/21/2025,"
beetle<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/NIU-203-scaled.jpg,",41.9295355,-88.74991279999999,07/21/2025,"
caddis fly<\/p>\n",Trichoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/NIU-202-scaled.jpg,",41.9295355,-88.74991279999999,07/21/2025,"
crane fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/NIU-201.jpg,",41.9295355,-88.74991279999999,07/21/2025,"
mosquito<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/NIU-200-scaled.jpg,",41.9295355000,-88.7499128000,07/21/2025,"
leafhopper<\/p>\n",Hemiptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_0195-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_0157-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/0EAF5240-7DDD-4B91-8CE3-07F4C7C2E058.jpg,",41.7756400000,-88.0970000000,09/04/2025,"
I used a sweep net to capture my insect which was found in a tall grass habitat in the quad.<\/p>\n",Melanoplus femurrubrum,DNeasy (Qiagen) blood and tissue kit.,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_8B5CB773-9C6D-4355-961C-C0BFE279334A-scaled.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Insect-1-scaled.jpeg,",41.7756400000,-88.0970000000,09/02/2025,"
$\"\"$ collected by Fall 2025 students<\/p>\n",Formica pallidefulva,DNeasy (Qiagen) blood and tissue kit.,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/gel-image-scaled.jpeg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Arthropd-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_9531.png,",35.7305306000,-81.6862142000,09/25/2025,"
I found this milkweed bug behind the residential building. It was found on a sunflower in the morning at about 11:00 am. There were most likely more milkweed bugs in the area but I only collected two. $\"\"$ <\/p>\n",Lygaeidae,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-03-at-10.33.55 AM.png,","
DNA Extraction:\u00a0<\/strong>I made sure to take off the exoskeleton and dissect the abdomen.<\/p>\n
Gel Lanes:<\/strong> Lane 1: 100bp Ladder
\nLane 2: (1A) 708 bp ~ Arthropod DNA
\nLane 3: (1W) No Band
\nLane 4: (2A) 708 bp ~ Arthropod DNA
\nLane 5: (2W) No Band
\nLane 10: (A+) 708 bp ~ Arthropod DNA
\nLane 11: (A-) No Band
\nLane 12: (W+) 438 bp ~ Wolbachia DNA
\nLane 13: (W-) No Band<\/p>\n
Analysis:\u00a0<\/strong>My controls worked and I had a band on my gel for my samples.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Grasshopper.webp,",41.7766153000,-88.0953734000,09/02/2025,"
Fall 2025 Students
\nCollected by: Beat Net<\/p>\n
Caught in: Tall grass\/Grass<\/p>\n
$\"\"$ <\/p>\n",Melanoplus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/gel.webp,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/tempImage7UzxDa-scaled.jpg,",41.7760574665,-88.0954555740,09/02/2025,"",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/tempImageCFufkC-scaled.jpg,","",No,TTTTATCAATAGAAAAATTTTTATGATGTATATTAATAATTGTTGAAATAAAATTAATAGCCCCTAAAATAGATGATATA CCGGCAATATGAAGAGAAAAAATTGTTAAGTCTACAGAAGGTCCATTATGAAAAATATTTGAAGCTAAAGGAGGGTAGAT AGTTCATCCAGTTCCAGTACCATCATTAATAAAGTTTCTTAAAAGTAATAGAGTGATGGAAGGAGGTAAAAGTCAAAATC TTATATTATTTATACGAGGATAAGCTATATCTGGTGACCCTAATATTAAAGGAATTAGAAAATTTCCAAATCCACCAATT ATAAATGGTATTACTATAAAAAAGATTATAATAAAGGCATGATTAGTTACTAAAGAATTATAAATTTGATCATTATTAAT TAATGAATTTGAAGAACCTAATTCTAATCGAATAATTATACTTATAGAAGATCCAATTATTCCAGCTCA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/6-DA_P3_CoR.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/997CBD20-2D7B-45CB-92A6-AA764B892B56-scaled.jpg,",41.7784418,-88.0952683,09/04/2025,"
Caught by Fall 2025 student at Benedictine University. Captured in the Quad by Kindlon Hall.<\/p>\n",Hemiptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"","
During the protocols there was lots of DNA. I had extra DNA after the PCR and needed to dilute.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_7320-scaled.jpeg,",41.7756400000,-88.0970000000,09/02/2025,"
collected by Fall 2025 students<\/p>\n",Vespula germanica,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",No,ATTATATTTTATTTTCGCTTTATGAGCAGGAACTTTAGGAGCTTCAATAAGAATAATTATTCGTTTAGAATTAAGATCCCCTGGAGCTTTAATTAATAATGATCAAATTTATAATACTATTATTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTTATACCTTTTTTAGTTGGAGGATTTGGAAATTGATTAATCCCTTTAATATTAGGTGTTCCTGATATAGCATTTCCTCGAATAAATAATATAAGATTTTGATTATTACCTCCATCCTTATTTTTATTAATCTTAAGAAATTTTATTGGAACCGGAGTAGGGACAGGATGAACTCTGTACCCTCCTTTATCATCAATTGTAGGACATGACTCTCCCTCTGTAGACTTAGGAATTTTTTCTATCCATATTGCTGGAATTTCATCAATTATAGGTTCAATTAATTTTATTGTTACTATTTTAAATATACACACAAAAACACATTCACTAAATTTTCTTCCTTTATTTTCATGATCAATTTTAATTACAGCAATTCTTCTCTTGTTATCTCTACCAGTTCTTGCAGGAGCAATTACTATACTTCTTACAGACCGTAATTTAAATA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_2869.jpeg,",41.7784418000,-88.0952683000,09/04/2025,"
Caught by Fall 2025 student at Benedictine University. Captured in the Quad by Krasa Hall.<\/p>\n",Orthoptera,DNeasy (Qiagen) blood and tissue kit.,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_3067-scaled.jpeg,","
During the protocols I did have some trouble since the beginning by not being able to transfer a lot of DNA and it was chunky. I had extra DNA after the PCR and needed to dilute it.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/1000000469-scaled.jpg,",41.7774583660,-88.0965217944,09/04/2025,"
Caught by Fall 2025 student of Benedictine University. Retrieved in grassy area near Krasa Hall.<\/p>\n",Orthoptera,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/1000000689-scaled.jpg,","
Everything went normal during protocols. I had extra DNA after PCR and needed to dilute it. My results went wrong after receiving my gel electrophoresis results.<\/p>\n",Unknown,TAAATCAACGAAGGTTCCTCCGGGACCATTATTTGAAGATAAGGGGGGATATACGGTTCTTCCGTATCCTGCCCCTCTTTCTACT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/65EB79F7-1B3B-473A-9C33-088D70DBF417.jpg,",41.7762331864,-88.0966740568,09/02/2025,"
Collected by Fall 2025 student<\/p>\n
By Kindlon Hall using a beat net and killing jar tall grassy area<\/p>\n",Oncopeltus fasciatus,DNeasy (Qiagen) blood and tissue kit.,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-02-at-1.37.42 PM.png,","
My wall is the fourth with little to no band, which means I did not have DNA<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_3900-scaled.jpeg,",41.7771440555,-88.0959950870,09/02/2025,"
collected by Fall 2025 students<\/p>\n",Sciaridae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_4059-scaled.jpeg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Andrew-B3.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/SamplePic2.jpg,",41.7772262301,-88.0957478956,09/04/2025,"
Collected by Fall 2025 students<\/p>\n",Tapinoma sessile,DNeasy (Qiagen) blood and tissue kit.,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"","
During the DNA extraction phase, the DNA sample for my original arthropod, Tapinoma sessile<\/em> (Odorous House Ant), was lost and could not be recovered. Because of this, there is no Wolbachia 16S sequence, no Wolbachia 16S fasta file, no Wolbachia 16S ab1 file, and no arthropod COI fasta file or COI ab1 file for this specimen. There is also no gel electrophoresis image for this individual. I was provided with a replacement arthropod specimen, Hymenoptera sp.<\/em>, and all downstream analyses including PCR, gel electrophoresis, Sanger sequencing, and BLAST identification were performed using the replacement specimen.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/anthropod-photo.jpg,",41.7756400000,-88.0970000000,09/04/2025,"
Collected by Fall 2025 students, found on concrete<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/gel-scaled.jpg,","",Yes,TCTTTGGTCTATGAGCTGGTATAATTGGAAGATCATTAAGATTAATTATTCGTTTAGAATTAGGAACACCTTTATCAATTATTAATAATGATCAAATCTATAATTCAATTATTACAGCTCACGCATTAATTATAATTTTTTTCATAGTTATACCATTTATAATTGGAGGATTTGGAAATTGATTAATTCCTTTAATATTAGGTGCCCCAGATATAGCATTTCCACGAATAAATAATATAAGATTTTGATTATTACCACCATCATTAATATTATTAATTATTAGAAATATTAACGATATAGGAGTAGGAACTGGATGAACTTTATATCCACCTTTATCATCTAATATAGGTCACAATTCATCATCAGTTGATTTTGCTATTTTCTCTCTTCACATTGCAGGAATTTCATCAATTATAGGAGCTATTAATTTCATTGTAACAATCTTAAATATACATATTAAAACTCATTCATTAAATTTTTTACCTATATTTACATGATCAGTTTTAATTACTGCAATTTTACTTTTATTATCTTTACCTGTATTAGCTGGAGCAATTACAATACTATTAACCGATCGAAATATTAATACATCTTTTTTTGATCCATTAGGAGGTGGAGACCCTATTTTATTCCAACATTTATTTTGATTTTTTGGTCANNNNNNNAANNTTTNAAA,,, "",19.7018367,-155.0791279,11/13/2025,"
A clover mite (Bryobia praetiosa<\/em>) collected from the compost area at Hawai\u2018i Community College tested positive for Wolbachia. A faint 16S rRNA band was visible on the agarose gel, confirming the presence of the bacterium.<\/p>\n",Arachnida,none,Whole arthropod,Standard electrophoresis system,lysis,Cyber Stain,"","
DNA was extracted from a clover mite (Bryobia praetiosa) using the standard Wolbachia Project lysis, precipitation, and elution procedure. The specimen was rinsed with water, transferred into a labeled microcentrifuge tube, and ground in Cell Lysis Buffer with a sterile pestle. The tube was heated, vortexed, and centrifuged to pellet debris. The supernatant was moved into a new tube containing cold isopropanol to precipitate DNA, followed by centrifugation and a 70 percent ethanol wash. The DNA pellet was air-dried and resuspended in DNA Elution Buffer. A single CO1 PCR and a single Wolbachia 16S rRNA PCR were prepared for the clover mite sample. PCR products were run on a 2 percent agarose gel cast with SYBR Safe dye in TBE buffer. A DNA ladder was included for size comparison. After electrophoresis, the gel was viewed under UV light. The clover mite produced a faint but visible band in the Wolbachia 16S lane, confirming infection.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0440-2.jpg,",41.7784418000,-88.0952683000,09/02/2025,"
Found infront of quad on the concrete right next to tall grass.<\/p>\n",Melanoplus femurrubrum,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",No,>91-CA_V4_CoR.ab1 (650 bp) GGATCACCTCCTCCCGCAGGGTCAAAGAATGATGTATTTAAATTTCGATCAGTTAATAATATTGTAATAGCTCCTGCTAA TACAGGAAGTGATAATAATAAAAGGAGTGCTGTAATAGCTACTGATCAAACAAATAATGGTGTTTGGTCTAGTGTTATTC TTTCTGATCGTATATTAATTGCTGTTGTAATAAAATTGACTGCCCCTAGAATTGATGAAACACCAGCTAAGTGAAGAGAG AAAATGGCTAAATCAACTGACGCTCCACCATGTGTAATTGCTCCAGCGAGTGGGGGGTAAACTGTTCATCCTGTACCAGC TCCATTATCAACCATAGATGAGGTAAGTAAAAGGGTTAGTGATGGTGGTAAAAGTCAAAAACTTATATTATTTATTCGTG GAAAAGCTATATCTGGTGCTCCAATTATTAATGGTACAAGTCAATTACCAAATCCTCCAATTATAATAGGTATTACTATA AAGAAAATTATTACAAATGCGTGGGCTGTAATAATTACATTATAGATTTGGTCATCTCCAATTAGAGATCCTGGTTGTCC TAGTTCTGCTCGAATAATTATTCTTATAGAAGTTCCTACTATTCCAGCTCATGCTCCAAATATAAAGTATAAGGTACCAA TATCTTTATG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_9822-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_9824-scaled.jpg,",42.4605952344,-73.3159554965,09/30/2025,"
The arthropod was caught while Being dragged behind by another ant on a trail, the trail was dry and void of any moisture.<\/p>\n",Camponotus,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_3686-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/image_2025-11-17_233928488.png,","
We used a DNA extraction protocol based on the insect adaptation of New England Biolabs’ Monarch Spin gDNA Extraction kit (Product # T3010).<\/span><\/p>\n
The specimen was incubated for 30 minutes in a hot water bath at 56 degrees C, today’s PCR was a <\/span>duplex<\/span> reaction because we used both the Arthropod CO1 and Wolbachia 16S primers. I <\/span>used an annealing temperature of<\/span> 49<\/span> degrees Celsius.\u00a0<\/span>used Taq polymerase: New England Biolabs OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer (#M0488S)<\/p>\n
$\"\"$ <\/p>\n
Gel electrophoresis system: Edvotek Gel Electrophoresis
\nBuffer: 1X TAE
\nDNA Stain: SYBR Safe<\/p>\n
My first gel Image was taken on 10-21-25 and was run at 125 Volts for 30 minutes Using New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S). This round told me that my arthropod tested false for Wolbachia<\/p>\n
Our second PCR reaction was set up on 10-28-25, used the same Taq polymerase as the first PCR reaction, and:<\/p>\n
was a Single.
\nWe used both the Arthropod CO1 and Wolbachia 16S primer(s).<\/p>\n
while using an annealing temperature of 55 degrees Celsius.<\/p>\n
Second Round:<\/p>\n
Gel electrophoresis system: Edvotek Gel Electrophoresis<\/span><\/p>\n
Buffer: 1X TAE\u00a0<\/span><\/p>\n
DNA Stain: SYBR Safe\u00a0<\/span><\/p>\n
Protocol notes: ADD the following statements:<\/span><\/p>\n
\n
Our <\/span>second<\/b> Gel image was taken on 11-4-25 and:\u00a0<\/span>\n
\n
was run at 125 volts for 30 minutes<\/span><\/li>\n
used this DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S)<\/span><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n
Results: I am Highly positive that my arthropod is not Wolbachia infested, the reason for this is because after running the single Twice to check for any errors It came out with the same results witch leads me to Say in confidence that my arthropod test negatively.<\/p>\n
This week we did DNA sequencing. For our arthropods only had the ZK-1 Come out due to the CD-1 arthropod being too\u00a0distorted and messy. $\"\"$ CO1: Sequence $\"\"$ S16: Sequence $\"\"$ <\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3296-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3295-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3294-scaled.jpeg,",42.7112633000,-73.1994775000,09/29/2025,"
I caught this infamous species in a close garden; it was consuming rotten tomatoes near by a composter.<\/p>\n",Halyomorpha halys,Monarch DNA extraction (NEB),Partial abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/original-2EC85BE0-19F0-4641-BAA7-3F6BF38AE87B.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_3527.jpeg,","
\n
We used a DNA extraction method based on the insect adaptation of New England Biolabs Monarch Spin gDNA Kit (# T3010)<\/li>\n
The specimen was incubated for 30 minutes in hot water at 56 degrees C.<\/li>\n
During the last step of the DNA extraction we added too much elution buffer.<\/li>\n
The PCR reaction held on 10\/16\/2025 and it was a duplex reaction in which we used Wolbachia 16S and Arthropod CO1 primers simultaneously.<\/li>\n
\u00a0TAQ Polymerase; New England Biolabs OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer (#M0488S) was utilized.<\/li>\n
Annealing temperature of 49 degrees C.<\/li>\n
Our first gel image was taken on 10\/23\/25, and it was run at 125volts for 25 minutes.<\/li>\n
New England Biolabs provided DNA ladder for Safe Stains (#N0559S).<\/li>\n<\/ul>\n
\n
PCR 2 – Gel Electro 2<\/p>\n
\n
The PCR reaction held on 11\/4\/2025 and it was a single reaction<\/li>\n
Annealing temperature of 55 degrees C.<\/li>\n
Our second gel image was taken on 11\/4 and was run at 125 volts for 25 minutes.<\/li>\n
The DNA ladder we use is, New England 1 kb plus DNA ladder for safe stains (#N0559S)<\/li>\n<\/ul>\n
Results –<\/p>\n
I am high-moderate confident that Arthropod 2 (Pillbug) is infected with Wolbachia. On the other hand, on the first PCR testing I determined that Arthropod 1 (Stink Bug) did not had Wolbachia. Today, I am moderate confident that Arthropod 1 may be infected.<\/p>\n
<\/p>\n",No,ATATGATCTGGAATAGTAGGATCAGCTATAAGATTAATTATCCGTATCGAATTAGGACAGCCTGGAAGATTTATTGGTAATGATCAAATTTATAATGTAATTGTAACAGCACATGCATTTGTAATAATTTTCTTTATAGTAATACCAATCATAATTGGAGGATTCGGTAATTGATTAGTCCCTTTAATAATTGGAGCACCTGATATAGCCTTCCCACGATTAAATAATATAAGATTCTGATTATTACCCCCTTCATTAACTTTATTATTAATAAGAAGAATAGCAGAATCAGGAGCTGGGACAGGATGAACAGTCTATCCCCCCTTATCAAGTAATATCTCACATAGAGGAGCATCAGTTGATTTAGCTATTTTTTCCTTACATTTGGCTGGGGTTTCATCAATTTTAGGGGCAGTAAATTTTATTTCAACTATTATAAATATACGACCAACAGGTATAACCCCTGAACGAATCCCATTGTTTGTGTGATCAGTAGGAATTACTGCACTTCTTCTGTTATTATCCTTACCTGTGTTAGCAGGTGCTATTACTATACTATTAACTGACCGAAATTTTAATACCTCTTTTTTTGACCCATCAGGAGGAGGAGACCCTATTCTTTATCAACATTTATTTTGA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_3221.JPG-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_2986.JPG-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_2878.JPG-scaled.jpeg,",41.7756400000,-88.0970000000,09/02/2025,"
Collected by fall 2025 students with a killing jar nearby trees and tall grass.<\/p>\n",Melanoplus,DNeasy (Qiagen) blood and tissue kit.,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","
DNA was isolated out of the insect abdomen with the use of the DNeasy (Qiagen) Blood and Tissue Kit in accordance with the standard protocol. Tissue was lysed in Buffer ATL containing Proteinase K and incubated until digested, then attached to a silica column, rinsed with Buffers AW1 and AW2 and washed off into AE buffer.<\/p>\n
A single reaction of PCR amplification was done and the 16S and COI amplifications were run separately under thermal cycling.\u00a0PCR products were separated on an agarose gel by the use of a standard electrophoresis system utilizing the TAE buffer. The DNA stain was SYBR Safe. Samples were loaded and a molecular weight was used to confirm anticipated band sizes. Electrophoresis was performed, followed by visualization of gels in the blue light transilluminator, and imaging was done.<\/p>\n
The presence or absence of the band was taken to assess the presence of Wolbachia in the insect and the samples with clear and distinct bands were sequenced. Where feasible, sequence quality and results of BLAST were done to establish the level of confidence in the identification of Wolbachia.<\/p>\n",No,>11-DA_V2_CoR.scf (571 bp) GTATTTAAATTTCGATCAGTTAATAATATTGTAATAGCTCCTGCTAATACAGGAAGTGATAATAATAAAAGGAGTGCTGT AATAGCTACTGATCAAACAAATAATGGTGTTTGGTCTAGTGTTATTCTTTCTGATCGTATATTAATTGCTGTTGTAATAA AATTGACTGCCCCTAGAATTGATGAAACACCAGCTAAGTGAAGAGAGAAAATGGCTAAATCAACTGACGCTCCACCATGT GCAATTGCTCCAGCGAGTGGGGGGTAAACTGTTCATCCTGTACCAGCTCCATTATCAACCATAGATGAGGTAAGTAAAAG GGTTAGTGATGGTGGTAAAAGTCAAAAACTTATATTATTTATTCGTGGAAAAGCTATATCTGGTGCTCCAATTATTAATG GTACAAGTCAATTACCAAATCCTCCAATTATAATAGGTATTACTATAAAGAAAATTATTACAAATGCGTGGGCTGTAATA ATTACATTATAGATTTGGTCATCTCCAATTAGAGATCCTGGTTGTCCTAGTTCTGCTCGAATAATTATTCTTATAGAAGT TCCTACTATTC,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/11-DA_V2_CoR.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0218.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0238.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0237.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0243-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0247-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0250-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0252-scaled.jpg,",42.1839000000,-73.4371400000,09/30/2025,"
Area around the specimen was damp, but the specimen was on a pretty dry flat rock.<\/p>\n
It was collected at 9:30pm, just off the road, in a big agricultural field.<\/p>\n
<\/p>\n",Armadillidium vulgare,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Gel1EditedWithNames.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Gel-Image-2-scaled-e1762913962722.jpg,","
We used a DNA extraction protocol based on the insect adaptation of New England Biolabs’ Monarch Spin gDNA Extraction kit (Product # T3010).<\/p>\n
The specimen was incubated for 30 minutes in a hot water bath at 56 degrees C.<\/p>\n
Details that differed from the written protocol (incubation time differences, accidental mistakes, etc.) were:<\/p>\n
\n
The proper quantity of the sample used for extraction was specified in the protocol as being smaller than a grain of rice. I accidentally used roughly 1.5x that amount.<\/li>\n
The protocol called for an incubation time of anywhere from 30-60 minutes, with times such as 2-3 hours being ideal. I only incubated mine for ~30 minutes. While 30 minutes should be long enough, as stated in the protocol, it would have been beneficial to incubate for longer.<\/li>\n<\/ol>\n
Our first PCR reaction was set up on 10\/15\/2025 and was a duplex reaction because we used both the Arthropod CO1 and Wolbachia 16S primers together.<\/p>\n
We used an annealing temperature of 49 degrees Celsius.<\/p>\n
We used this Taq polymerase: New England Biolabs OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer (#M0488S).<\/p>\n
Differed from the written protocol?<\/p>\n
\n
Our total amount of Taq Master Mix for the 7 reactions was 88ul, not 87.5ul. This was due to our micropipette not being able to measure out decimals, so we had to round up to 88ul.<\/li>\n<\/ul>\n
Our first Gel image was taken on 10\/22\/2025 and:<\/p>\n
\n
Was run at 125 volts for 20 minutes.<\/li>\n
Used this DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S).<\/li>\n<\/ul>\n
Our second PCR reaction was set up on 10\/29\/2025, used the same Taq polymerase as the first PCR reaction, and was a duplex reaction that used the Arthropod CO1_F&R and Wolbachia 16S_F&R primers.<\/p>\n
\n
We used an annealing temperature of 49\u00baC.<\/li>\n
Differed from the standard protocol in that I used 2.5uL of template DNA from my sample instead of using 2.0uL like the protocol calls for. To ensure that all of the PCR tubes contained the proper total volume of 25uL (DNA + PCR cocktail), I added the water to each PCR tube separate from the PCR cocktail. The PCR cocktail contained no water. Every control received 2.5uL of water, along with the proper 2.0uL of template DNA. My sample received 2.0uL of water, along with the increased 2.5uL of template DNA. The total volume of PCR cocktail added to each tube was 20.5uL.<\/li>\n<\/ul>\n
Our second Gel image was taken on 11\/5\/2025 and was run at 125 volts for 21 minutes.<\/p>\n
\n
\u00a0Used this DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S).<\/li>\n<\/ul>\n",No,GAGCAGGGGCTGTTGGGACTGCTCTTAGAATAATTATTCGCACTGAATTAGGACAACCTGGAAGTTTAATTGGAGATGATCAGATTTATAATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATACCTATTATAATTGGTGGATTCGGTAATTGGTTAATTCCATTAATACTAGGAGCCCCAGATATAGCTTTTCCACGAATAAATAATATAAGATTTTGACTCTTACCCCCTTCTTTAACTTTATTACTGAGAAGGGGATTAGTTGAAAGAGGAGTAGGAACAGGGTGAACAGTGTATCCTCCGCTGGCGTCAAGAATTGCTCATAGAGGAGCATCTGTAGATTTAGGTATTTTTTCTCTCCACCTTGCTGGAGCCTCTTCTATTTTAGGGGCTGTAAATTTTATTACTACTATAATTAATATACGAGCAGCTGGAATTAGAATAGACCGTGTTCCTTTATTTGTTTGATCAGTAATAGTAACGGCTGTACTTTTACTTTTATCATTACCTGTATTAGCAGGAGCTATTACTATATTATTGACTGATCGAAATTTAAATACATCTTTTTTTGACCCTAGAGGAGGGGGGGATCCTGTCCTTTATCAACATTTATTTTGA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3111-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3124-scaled.jpeg,",38.9423241937,-77.0618864692,09/04/2025,"
Orange-red lady beetle with black spots. To short, straight antennae. 6 legs. 2 wings. Circular abdomen. Small head.<\/p>\n
Found inside the school.<\/p>\n
Late summer<\/p>\n
Temperature: 76\u00ba F<\/p>\n
Habitat: urban<\/p>\n",Coleoptera,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0009.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_3002.jpeg,","
DNA extraction: Used the whole arthropod. Everything went as planned.<\/p>\n
PCR: We added two times the listed amount of ATL buffer and 10 extra microliters of Proteinase K. We had to split the sample into two tubes during DNA extraction and respin them several times. We recombined the tubes at the beginning of the PCR lab.<\/p>\n
Gel electrophoresis, Arthropod CO1: in lane 1 was the DNA ladder. C-1 was in lane 2, C-2 was in lane 3, C-3 was in lane 4, C-4 was in lane 5, W+ was in lane 6, W- was in lane 7, H2O control was in lane 8. No mistakes were made. Controls worked.<\/p>\n
Gel electrophoresis\u00a0Wolbachia<\/em>: DNA ladder in lane 1, C-1 in lane 2, C-2 in lane 3, C-3 in lane 4, C-4 in lane 5, W+ was in lane 6, W- was in lane 7, H2O control was in lane 8. No mistakes were made. Controls worked.<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/C4CO1-CO1F_R-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Screen-Shot-2025-10-28-at-10.13.57-PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Screen-Shot-2025-10-28-at-10.14.29-PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Screen-Shot-2025-10-28-at-10.14.18-PM.png,",38.7526000000,-76.9589000000,09/13/2025,"
Caught on a concrete path, with grass on each side of it close to a building. The concrete is a light color. Located in a woody, suburban area, with many types of different vegetation.\u00a0<\/span><\/p>\n
Caught late summer\/early starts of fall<\/span><\/p>\n
Temperature: High of 82\u2109; Low of 62\u2109<\/span><\/p>\n
Terrestrial Habitat<\/span><\/p>\n
Color: The arthropod is a rusty reddish brown color, with some areas being lighter than the others. Additionally, compared to it when it was first caught, it appears that it lost some of its color after being in the alcohol.\u00a0<\/span><\/p>\n
Wings: No wings<\/span><\/p>\n
Legs: This arthropod has 6 legs, with 3 on each side. The forelegs are connected to the thorax and look to be a little shorter than the others, however, it was hard to tell since the arthropod curled up after being in the freezer and alcohol. The legs look to be segmenting, with certain areas having joints where the legs bend. The hind legs are very similar to the fore legs, with the only difference being that they look to be proportional to the body length and they are the longest legs out of the 3 pairs.\u00a0<\/span><\/p>\n
Antennae: Has two antennae, which are elbow shaped. In other words, they are divided into what looks to be 2 segments, with one segment seeming longer than the other.\u00a0<\/span><\/p>\n
Body: There are 3 apparent body regions: the head, thorax, and abdomen. Each of the segments seems to be rounded. The head has a pair of mandibles, antennae, eyes, and a really small mouth that is hard to see. The thorax and abdomen seem to be connected by a small waist type situation containing two different sections. The body is covered by an exoskeleton. Additionally, the abdomen looks to have something maybe a little pointy or sharp on it, however, it can be part of the exoskeleton.<\/span><\/p>\n",Formica incerta,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Screen-Shot-2025-10-28-at-10.02.30-PM-3.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screen-Shot-2025-11-04-at-1.36.46-PM-1.png,","
DNA Extraction:\u00a0<\/span><\/p>\n
For this process, all of the arthropods were used. During this, this sample did have some type of bubbles before we incubated the sample, though it wasn\u2019t as much as other samples. Additionally, this sample solution was a tanish color.\u00a0<\/span><\/p>\n
<\/p>\n
PCR:<\/span><\/p>\n
For the PCR process, we did a CO1 one and a Wolbachia one. In the overall PCR process, there was a mistake where the DNA of 2 of the 6 samples was not added. However,\u00a0 this particular sample wasn\u2019t one of those, leading to no known mistakes for A-6.\u00a0<\/span><\/p>\n
<\/p>\n
Gel electrophoresis, Arthropod CO1:<\/span><\/p>\n
We used two different gel electrophoresis machines.\u00a0<\/span><\/p>\n
For machine 1:<\/span><\/p>\n
Lane 1 – DNA Ladder\u00a0<\/span><\/p>\n
Lane 2 – Arthropod A-1\u00a0<\/span><\/p>\n
Lane 3 – Arthropod A-2\u00a0<\/span><\/p>\n
Lane 4 – Arthropod A-3<\/span><\/p>\n
Lane 5 – Arthropod A-4<\/span><\/p>\n
Lane 6 – Wolbachia + control<\/span><\/p>\n
Lane 7 – Wolbachia – control<\/span><\/p>\n
Lane 8 – Water\u00a0<\/span><\/p>\n
Lane 9 – Blank\u00a0<\/span><\/p>\n
<\/p>\n
For machine 2:\u00a0<\/span><\/p>\n
Lane 1 – DNA Ladder\u00a0<\/span><\/p>\n
Lane 2 – Arthropod A-5\u00a0<\/span><\/p>\n
Lane 3 – Arthropod A-6\u00a0<\/span><\/p>\n
Lane 4 – Wolbachia + control<\/span><\/p>\n
Lane 5 – Wolbachia – control<\/span><\/p>\n
Lane 6 – Water\u00a0<\/span><\/p>\n
Lane 7 – Blank\u00a0<\/span><\/p>\n
Lane 8 – Blank<\/span><\/p>\n
Lane 9 – Blank<\/span><\/p>\n
<\/p>\n
All of the controls worked, with the Wolbachia (+) control and Wolbachia (-) control having the CO1 band and the water control having no band, meaning no contamination was made. Meaning that any mistakes made, in other words no bands showing up for arthropod sample, was because of an error in either the PCR process or loading the gel electrophoresis machine. This sample did have a band show up that was readable and in the correct location, being at 708bp. However, we continue with the results from the gel since the band was at the correct location.<\/span><\/p>\n
<\/p>\n
Gel electrophoresis, Wolbachia:<\/span><\/p>\n
We used two different gel electrophoresis machines.\u00a0<\/span><\/p>\n
For machine 1:<\/span><\/p>\n
Lane 1 – DNA Ladder\u00a0<\/span><\/p>\n
Lane 2 – Arthropod A-1\u00a0<\/span><\/p>\n
Lane 3 – Arthropod A-2\u00a0<\/span><\/p>\n
Lane 4 – Arthropod A-3<\/span><\/p>\n
Lane 5 – Arthropod A-4<\/span><\/p>\n
Lane 6 – Wolbachia + control<\/span><\/p>\n
Lane 7 – Wolbachia – control<\/span><\/p>\n
Lane 8 – Water\u00a0<\/span><\/p>\n
Lane 9 – Blank\u00a0<\/span><\/p>\n
<\/p>\n
For machine 2:\u00a0<\/span><\/p>\n
Lane 1 – DNA Ladder\u00a0<\/span><\/p>\n
Lane 2 – Arthropod A-5\u00a0<\/span><\/p>\n
Lane 3 – Arthropod A-6\u00a0<\/span><\/p>\n
Lane 4 – Wolbachia + control<\/span><\/p>\n
Lane 5 – Wolbachia – control<\/span><\/p>\n
Lane 6 – Water\u00a0<\/span><\/p>\n
Lane 7 – Blank\u00a0<\/span><\/p>\n
Lane 8 – Rerun Arthropod A-1<\/span><\/p>\n
Lane 9 – Rerun Arthropod A-2<\/span><\/p>\n
<\/p>\n
The reruns at the end of the machine 2 gel electrophoresis were because no CO1 band showed up on that gel for those samples due to the DNA being forgotten in the PCR process. All of the controls worked, with the Wolbachia (+) control having a Wolbachia band at 438bp\u00a0 and the Wolbachia (-) control and the water control having no band, meaning no contamination was made. Meaning that any mistakes made, in other words no bands showing up for arthropod sample, was because of an error in either the PCR process or loading the gel electrophoresis machine. This sample did have a Wolbachia band that showed up, meaning that this arthropod was positive for Wolbachia.<\/span><\/p>\n",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/A6CO1-CO1F_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Screen-Shot-2025-10-16-at-9.44.49-AM.png,",38.7526000000,-76.9589000000,09/06/2025,"
Caught in an open garage in a grassy, woody, suburban area. Outside the garage has many types of different vegetation, along with many concrete or asphalt paths.<\/p>\n
Caught late summer\/early starts of fall<\/p>\n
Temperature: High of 91F; Low of 68<\/p>\n
Terrestrial Habitat<\/p>\n
Color: Before putting it in the alcohol, its color was a tanish brown, with some areas being a darker brown or black. After being put into the alcohol, the color in a number of areas changed to a blue and purple color scheme.<\/p>\n
Wings: No wings<\/p>\n
Legs: Many legs, more than 8, that go around most of the arthropod’s body. The forelegs are just behind the head. The hind legs<\/p>\n
Antennae:\u00a0 2 long and slender antennae, looking similar to a piece of thread.<\/p>\n
Body: The body seems to have a more oval shape. However, it does seem to be segmented somewhat into two sections: the head and the body. The head is a rounded shape, containing compounded eyes, long antennas, and forcipules. The mouth is also small, with the forcipules begin behind the mouth. It doesn\u2019t have a distinct thorax or abdomen.\u00a0 <\/span><\/p>\n
<\/p>\n",Scutigera coleoptrata,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Screen-Shot-2025-10-28-at-10.02.30-PM-2.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screen-Shot-2025-11-04-at-1.36.46-PM.png,","
DNA Extraction:\u00a0<\/span><\/p>\n
For this process, only the abdomen was used. During this, this sample did have some type of bubbles before we incubated the sample. Additionally, this sample solution was a purplish blue color, which differed from the tanish color of the others. No mistakes were made for this sample that we are aware of.\u00a0<\/span><\/p>\n
<\/p>\n
PCR:<\/span><\/p>\n
For the PCR process, we did a CO1 one and a Wolbachia one. In the overall PCR process, there was a mistake where the DNA of 2 of the 6 samples was not added for the PCR one. However, this particular sample wasn\u2019t one of those, leading to no known mistakes for A-5.\u00a0<\/span><\/p>\n
<\/p>\n
Gel electrophoresis, Arthropod CO1:<\/span><\/p>\n
We used two different machines for the CO1 gel electrophoresis machines.\u00a0<\/span><\/p>\n
For machine 1:<\/span><\/p>\n
Lane 1 – DNA Ladder\u00a0<\/span><\/p>\n
Lane 2 – Arthropod A-1\u00a0<\/span><\/p>\n
Lane 3 – Arthropod A-2\u00a0<\/span><\/p>\n
Lane 4 – Arthropod A-3<\/span><\/p>\n
Lane 5 – Arthropod A-4<\/span><\/p>\n
Lane 6 – Wolbachia (+) control<\/span><\/p>\n
Lane 7 – Wolbachia (-) control<\/span><\/p>\n
Lane 8 – Water\u00a0<\/span><\/p>\n
Lane 9 – Blank\u00a0<\/span><\/p>\n
<\/p>\n
For machine 2:\u00a0<\/span><\/p>\n
Lane 1 – DNA Ladder\u00a0<\/span><\/p>\n
Lane 2 – Arthropod A-5\u00a0<\/span><\/p>\n
Lane 3 – Arthropod A-6\u00a0<\/span><\/p>\n
Lane 4 – Wolbachia (+) control<\/span><\/p>\n
Lane 5 – Wolbachia (-) control<\/span><\/p>\n
Lane 6 – Water\u00a0<\/span><\/p>\n
Lane 7 – Blank\u00a0<\/span><\/p>\n
Lane 8 – Blank<\/span><\/p>\n
Lane 9 – Blank<\/span><\/p>\n
<\/p>\n
All of the controls worked, with the Wolbachia (+) control and Wolbachia (-) control having the CO1 band and the water control having no band, meaning no contamination was made. Meaning that any mistakes made, in other words no bands showing up for arthropod sample, was because of an error in either the PCR process or loading the gel electrophoresis machine. This sample did have a band show up that was readable, however, it did appear smudged. However, we continue with the results from the gel since the band was at the correct location, which is 708bp.<\/span><\/p>\n
<\/p>\n
Gel electrophoresis, Wolbachia:<\/span><\/p>\n
We used two different machines for the Wolbachia gel electrophoresis machines.\u00a0<\/span><\/p>\n
For machine 1:<\/span><\/p>\n
Lane 1 – DNA Ladder\u00a0<\/span><\/p>\n
Lane 2 – Arthropod A-1\u00a0<\/span><\/p>\n
Lane 3 – Arthropod A-2\u00a0<\/span><\/p>\n
Lane 4 – Arthropod A-3<\/span><\/p>\n
Lane 5 – Arthropod A-4<\/span><\/p>\n
Lane 6 – Wolbachia + control<\/span><\/p>\n
Lane 7 – Wolbachia – control<\/span><\/p>\n
Lane 8 – Water\u00a0<\/span><\/p>\n
Lane 9 – Blank\u00a0<\/span><\/p>\n
<\/p>\n
For machine 2:\u00a0<\/span><\/p>\n
Lane 1 – DNA Ladder\u00a0<\/span><\/p>\n
Lane 2 – Arthropod A-5\u00a0<\/span><\/p>\n
Lane 3 – Arthropod A-6\u00a0<\/span><\/p>\n
Lane 4 – Wolbachia + control<\/span><\/p>\n
Lane 5 – Wolbachia – control<\/span><\/p>\n
Lane 6 – Water\u00a0<\/span><\/p>\n
Lane 7 – Blank\u00a0<\/span><\/p>\n
Lane 8 – Rerun Arthropod A-1<\/span><\/p>\n
Lane 9 – Rerun Arthropod A-2<\/span><\/p>\n
<\/p>\n
The reruns at the end of the machine 2 gel electrophoresis were because no CO1 band showed up on that gel for those samples due to the DNA being forgotten in the PCR process. All of the controls worked, with the Wolbachia (+) control having a Wolbachia band at 438bp\u00a0 and the Wolbachia (-) control and the water control having no band, meaning no contamination was made. Meaning that any mistakes made, in other words no bands showing up for arthropod sample, was because of an error in either the PCR process or loading the gel electrophoresis machine. This sample did not have a Wolbachia band that showed up, however like in the CO1 gel electrophoresis, it did appear smudged. However, even though the gel was smudge, we concluded that the sample was negative after looking at it more and a professional option.\u00a0<\/span><\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/image_2025-11-11_090154642.png,",39.0844971000,-77.1460727000,09/11/2025,"
I caught this spotted lantern fly at the Rockville metro station. There was several of them crowded along the walkway and I used my glasses case to capture it. The temperature was 67 degrees F. This is a terrestrial organism and hangout out all over city areas. Color Black, Grey, Red, White, Brown, 6 legs, 2 pairs of wings, No antennae, triangular head shape, oval like body.<\/p>\n",Lycorma,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/image_2025-11-04_134522619.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/image_2025-11-04_134133616.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/image_2025-10-16_100644768.png,","
PCR<\/p>\n
DNA was not properly included during this process. This resulted in a blank gel electrophoresis.<\/p>\n
Gel electrophoresis, Arthropod CO1<\/p>\n
Lane one contained DNA Ladder, lane two contained A-1 (the hornet), lane 3 contained A-2, these were the only two that did not show up well. I believe the gel electrophoresis worked properly because it reflected a previous mistake during the PCR process. DNA wasn’t properly included resulting in no results during gel electrophoresis. Everything else worked well.<\/p>\n
Gel electrophoresis, Wolbachia<\/p>\n
Lane one contained DNA Ladder, lane two contained A-1 (the hornet), lane 3 contained A-2, lane 3 contained A-3, lane 4 contained A-4, lane 5 contained Wolbachia positive DNA, lane 6 contained Wolbachia negative DNA, lane 7 contained water. Each control worked and outside of A-1 and A-2, no mistakes appeared to have been made.<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/A2CO1-CO1_SLF_43F_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/image_2025-10-16_095227894.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/image_2025-10-16_095210565.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/image_2025-10-16_095146969.png,",39.1009600000,-77.1624000000,09/09/2025,"
I caught this hornet in my second floor bathroom. The season was early fall\/late summer. The temperature was about 65 degrees F. This is typically a terrestrial organism but was in my home. Color: Black and Yellow,\u00a0 6 legs, 1 pair of wings,
\nantennae length of head,\u00a0 eggshaped abdomen
\nsharp pinsers.<\/p>\n",Hymenoptera,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/image_2025-10-16_100644768.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/image_2025-11-04_134522619.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/image_2025-11-04_134133616.png,","
PCR<\/p>\n
DNA was not properly included during this process. This resulted in a blank gel electrophoresis.<\/p>\n
Gel electrophoresis, Arthropod CO1<\/p>\n
Lane one contained DNA Ladder, lane two contained A-1 (the hornet), lane 3 contained A-2, these were the only two that did not show up well. I believe the gel electrophoresis reflected an issue regarding the PCR process, the DNA did not show up well due to a lack of DNA in the PCR. Everything else worked well.<\/p>\n
Gel electrophoresis, Wolbachia<\/p>\n
Lane one contained DNA Ladder, lane two contained A-1 (the hornet), lane 3 contained A-2, lane 3 contained A-3, lane 4 contained A-4, lane 5 contained Wolbachia positive DNA, lane 6 contained Wolbachia negative DNA, lane 7 contained water. Each control worked and outside of A-1 and A-2, no mistakes appeared to have been made.<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/WaspA1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_1221.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_1220.jpeg,",38.9441200000,-77.0644070000,09/11/2025,"
Location Description: Specimen collected while it sat on the railing of a terrace.<\/p>\n
Season: Autumn<\/p>\n
Temperature: 79 Fahrenheit<\/p>\n
Habitat: Urban<\/p>\n
Notes: Appears to be a spotted lanternfly, has two large brownish wings, a grey body\/head, and six legs.<\/p>\n
<\/p>\n",Hemiptera,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_1233-2.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_1232-2.jpeg,","
DNA Extraction: No errors made. Changed pipette tips between each sample.<\/p>\n
PCR: Pipette tip still held a bit of DNA solution after depositing 2 uL into the B-3 0.2 mL PCR Tube.<\/p>\n
Gel electrophoresis, Arthropod CO1: In Lane 1 is a DNA Ladder, in Lane 2 is Sample #1, in Lane 3 is Sample #2, in Lane 4 is Sample #3, in Lane 5 is Sample #4, in Lane 6 is a Positive Wolbachia Control, in Lane 7 is a Negative Wolbachia Control, and in Lane 8 is Water. No errors were made during this procedure and controls worked as intended.<\/p>\n
Gel electrophoresis, Wolbachia<\/em>: In Lane 1 is a DNA Ladder, in Lane 2 is Sample #1, in Lane 3 is Sample #2, in Lane 4 is Sample #3, in Lane 5 is Sample #4, in Lane 6 is a Positive Wolbachia Control, in Lane 7 is a Negative Wolbachia Control, and in Lane 8 is Water. No errors were made during this procedure and controls worked as intended.<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/B1CO1-CO1_SLF_43F_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3129-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3132-scaled.jpeg,",38.9420339786,-77.0616027382,09/09/2025,"
Spotted lantern fly. Gray wings, red underwings with white spots and brown tips. Black body. Long leg-like appendage coming out of the butt, 6 black legs. $\"\"$ <\/p>\n
Caught in the alley near the school.<\/p>\n
Early fall<\/p>\n
Temperature: 69\u00ba and sunny<\/p>\n
Habitat: urban<\/p>\n",Hemiptera,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0012.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_3003.jpeg,","
In DNA extraction the filter was clogged. We had to centrifuge it at a higher speed (10000 cpm), and multiple times. Some liquid had to be removed from the tube. We split it into two tubes then recombined the extracted DNA.<\/p>\n
PCR: all went well<\/p>\n
Gel electrophoresis, Arthropod CO1: DNA ladder in lane 1, C-5 in lane 2, C-6 in lane 3, C-6 in lane 4 (accidentally split C-6 into two lanes, but both showed the same results and there was no contamination.) W+ in lane 5, W- in lane 6, H2O control in lane 7. Controls worked.<\/p>\n
Gel electrophoresis, Wolbachia <\/em>16S: DNA ladder in lane 1, C-5 in lane 2, C-6 in lane 3, W+ in lane 4, W- in lane 5, H2O control in lane 6. No mistakes were made. Controls worked.<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/C5CO1-CO1_SLF_43F_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_1222.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_1224.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_1225.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_1226.jpeg,",38.9996800000,-77.1179200000,09/13/2025,"
Location Description: Specimen collected as it sat on a windowsill within a building.<\/p>\n
Season: Autumn<\/p>\n
Temperature: 85 Fahrenheit<\/p>\n
Habitat: Urban<\/p>\n
Notes: Appears to be a bottle fly, has a greenish hue, two wings, and six legs.<\/p>\n",Diptera,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_1233-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_1232-1.jpeg,","
DNA Extraction: No errors made. Changed pipette tips between each sample.<\/p>\n
PCR: Pipette tip still held a bit of DNA solution after depositing 2 uL into the B-3 0.2 mL PCR Tube.<\/p>\n
Gel electrophoresis, Arthropod CO1: In Lane 1 is a DNA Ladder, in Lane 2 is Sample #1, in Lane 3 is Sample #2, in Lane 4 is Sample #3, in Lane 5 is Sample #4, in Lane 6 is a Positive Wolbachia Control, in Lane 7 is a Negative Wolbachia Control, and in Lane 8 is Water. No errors were made during this procedure and controls worked as intended.<\/p>\n
Gel electrophoresis, Wolbachia<\/em>: In Lane 1 is a DNA Ladder, in Lane 2 is Sample #1, in Lane 3 is Sample #2, in Lane 4 is Sample #3, in Lane 5 is Sample #4, in Lane 6 is a Positive Wolbachia Control, in Lane 7 is a Negative Wolbachia Control, and in Lane 8 is Water. No errors were made during this procedure and controls worked as intended.<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/B2CO1-CO1F_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_1229.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_1228.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_1227.jpeg,",38.9996800000,-77.1179200000,09/09/2025,"
Location Description: Specimen collected as it was landing on a flower in an open grassy field.<\/p>\n
Season: Autumn<\/p>\n
Temperature: 75 Fahrenheit<\/p>\n
Habitat: Terrestrial<\/p>\n
Notes: Yellowish and black hue, two wings, six legs, and two short antennae.<\/p>\n",Hymenoptera,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_1232.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_1233.jpeg,","
DNA Extraction: No errors made. Changed pipette tips between each sample.<\/p>\n
PCR: Pipette tip still held a bit of DNA solution after depositing 2 uL into the B-3 0.2 mL PCR Tube.<\/p>\n
Gel electrophoresis, Arthropod CO1: In Lane 1 is a DNA Ladder, in Lane 2 is Sample #1, in Lane 3 is Sample #2, in Lane 4 is Sample #3, in Lane 5 is Sample #4, in Lane 6 is a Positive Wolbachia Control, in Lane 7 is a Negative Wolbachia Control, and in Lane 8 is Water. No errors were made during this procedure and controls worked as intended.<\/p>\n
Gel electrophoresis, Wolbachia<\/em>: In Lane 1 is a DNA Ladder, in Lane 2 is Sample #1, in Lane 3 is Sample #2, in Lane 4 is Sample #3, in Lane 5 is Sample #4, in Lane 6 is a Positive Wolbachia Control, in Lane 7 is a Negative Wolbachia Control, and in Lane 8 is Water. No errors were made during this procedure and controls worked as intended.<\/p>\n",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/B3CO1-CO1F_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_5819-scaled.jpeg,",36.0169705363,-84.2620567472,10/15/2025,"
Big<\/p>\n
small wings<\/p>\n",Habropoda laboriosa,pcr,Abdomen,MiniPCR,lysis,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_5870-scaled.jpeg,","",No,,,, "",40.5584419400,-75.4049716700,10/16/2025,"
white-jawed jumping spider (Hentzia mitrara)<\/p>\n
Praying Mantis<\/p>\n
European Harvestman Spider<\/p>\n
Banded Tussock Moth Caterpillar<\/p>\n",Arachnida,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,SYBR Safe,"","
Line 1 Hentz Spider: has Wolbachia<\/p>\n
Line 2 Praying Mantis: has Wolbachia<\/p>\n
Line 3<\/p>\n
Line 4<\/p>\n
Line 5<\/p>\n
Line 6<\/p>\n
Line 7<\/p>\n
Line 8 Empty<\/p>\n
Line 9 Harvestmen Spider: no Wolbachia<\/p>\n
Line 10 Caterpillar: no Wolbachia<\/p>\n
Line 11<\/p>\n
Line 12<\/p>\n
Line 13<\/p>\n
Line 14<\/p>\n
Line 15<\/p>\n
Line 16 Empty<\/p>\n",Yes,GGNNGCCTGATCTGCTATAGTGGGTATAGCAATAAGAGTATTGATTCGAATAGAATTAGGTCAAACTGGA TCATTTTTAGGTAATGATCATATATATAATGTTATTGTTACAGCTCATGCTTTTGTTATAATTTTTTTTATAGTAATGCC TATTTTAATTGGAGGTTTTGGAAATTGGTTGGTTCCTTTGATATTAGGGGCTCCTGATATAGCTTTTCCTCGGATAAATA ATTTAAGTTTTTGATTATTACCCCCTTCATTATTATTGTTATTTATTTCTTCAATAGCAGAAATAGGAGTTGGGGCTGGA TGAACTGTTTATCCCCCTTTAGCTTCTATTGTTGGTCATAATGGAAGATCTGTAGATTTTGCTATTTTTTCTTTACATCT AGCAGGTGCTTCTTCAATTATAGGAGCTATTAATTTTATTTCAACTATTATTAATATACGTTCTATTGGAATATCTTTAG ATAAGATTCCTCTTTTTGTTTGATCAGTTATAATTACGGCTGCTCTATTACTTTTATCATTACCAGTGTTAGCTGGAGCT ATTACTATATTATTAACTGATCGAAATTTTAATACTTCCTTTTTTGATCCAGCAGGAGGAGGAGATCCTATTTTATTTCA ACATTTATTTTGATTTTTTGGNCA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-18-at-6.47.19 PM-1.png,",41.775990182124026,-88.09635863996918,09/02/2025,"
I found the Carolina Grasshopper in front of my university’s parking garage, on the wall by the entrance. I used a sweep net to catch it. It was in the small flower garden that’s in the middle of the pathway by the entrance, and I chased it all the way to the parking garage.<\/p>\n",Dissosteira carolina,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-18-at-5.54.00 PM.png,","",Yes,AGCATTAATGAATGATATAGGATTGTGATCTCCACCTCCAGCTGGATCAAAGAATGATGTATTAATATTACGGTCTGTTAATAGTATTGTAATTGCTCCAGCTAAAACTGGTAATGATAATAGTAATAATAATGCTGTAATTGCTACTGATCAAACAAATAAGGGTGTTTGATCTATAGTTATTCTATCTGATCGTATATTAATTGCTGTTGTAATGAAGTTTACTGCTCCTAAAATTGATGAAATACCTGCTAAGTGTAGTGAAAAAATAGCTAAATCTACTGATGCCCCTCCATGTGCAATTGCTCCTGCTAATGGTGGGTAGACTGTTCATCCTGTACCTACCCCTCTATCAATTATTCTAGATGAGAGTAGTAAAATTAATGATGGTGGTAATAATCAAAATCTTATATTATTTATTCGTGGAAATGCTATATCTGGTGCCCCAATTATTAATGGTACTAGCCAATTTCCAAAACCCCCAATCATGATTGGTATAACTATGAAGAAGATTATAACAAATGCATGTGCTGTGATAATAACATTATAAATTTGGTCATCTCCAATCATAGATCCTGGTTGCCCTAATTCAGCCCGAATAATTATTCTTATAGATGTTCCAACTATACCTGCTCATGCTCCAAATATAAAGTATAAAGTTCCAATATCTTTATGATTTGTTGACC,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Updated2-CA_B2_CoR.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/GOOBER-DRAWING-scaled.jpg,",,,08/26/2025,"
\n
Greenhouse fenced in, contained tall grasses, abundance of greenery and trees<\/span><\/li>\n
Environment: Hot and humid<\/span><\/li>\n
Method of Collection: Sweep-Net<\/span><\/li>\n<\/ul>\n",Homalodisca vitripennis,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Gel-Electrophoresis.jpg,","
A DNA extraction kit of in-house reagents was used.<\/p>\n",No,TTGATCTGGTATAGTTGGTATAATATTAAGAATAATTATTCGAATTGAAT TAGCTCaacctgtaggcgtttTTAATAAATGATCAATTATATAATGTAAT TGTTACTtcaCATGCTTTTATCATAATTTTTTTTATAGTTATACCAATTA TAATTGGTGGATTTGGTAATTGATTATTACCTTTAATAATTGGTGCTCCT GATATAGCATTTCCACGAATAAATAATATAAGATTTTGATTGTTACCCCC TTCATTATCTTTGTTATTAGTAAGATCAATAGTTGAAATAGGTGTTGGAA CAGGATGAACTGTTTACCCACCACTATCTTCTAATGTGGCTCATTCAGGG CCAAGAGTTGATTTAGCTATTTTTTCATTACATTTAGCAGGTATTTCATC AATTTTAGGAGCTGTAAATTTTATTACAACAGTAATTAATATACGAGCTA TTGGAATAAATTTAGATCGTACTCCTTTATTTGTTTGATCAGTATTAATT ACTGCTATTTTATTATTACTTTCATTACCTGTCTTAGCAGGTGCTATTAC TATATTATTAACTGATCGAAATATTAATACATCTTTTTTTGATCCTTCTG GTGGAGGAGATCCTATTTTATACCAACACTTATTTTGATTTTTTGGTCAC CctgaAGTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/E6BF32B7-65EB-45E0-9E84-C0D4D86BCF0B-scaled.jpg,",41.7771206849,-88.0960936305,09/02/2025,"
Founded in grass area and sidewalk<\/p>\n",Acheta domesticus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",No,>89-CA_V1_CoR.ab1 (641 bp) CCTCCTCCCGCAGGGTCAAAGAATGATGTATTTAAATTTCGATCAGTTAATAATATTGTAATAGCTCCTGCTAATACAGG AAGTGATAATAATAAAAGGAGTGCTGTAATAGCTACTGATCAAACAAATAATGGTGTTTGGTCTAGTGTTATTCTTTCTG ATCGTATATTAATTGCTGTTGTAATAAAATTGACTGCCCCTAGAATTGATGAAACACCAGCTAAGTGAAGAGAGAAAATG GCTAAATCAACTGACGCTCCACCATGTGCAATTGCTCCAGCGAGTGGGGGGTAAACTGTTCATCCTGTACCAGCTCCATT ATCAACCATAGATGAGGTAAGTAAAAGGGTTAGTGATGGTGGTAAAAGTCAAAAACTTATATTATTTATTCGTGGAAAAG CTATATCTGGTGCTCCAATTATTAATGGTACAAGTCAATTACCAAATCCTCCAATTATAATAGGTATTACTATAAAGAAA ATTATTACAAATGCGTGGGCTGTAATAATTACATTATAGATTTGGTCATCTCCAATTAGAGATCCTGGTTGTCCTAGTTC TGCTCGAATAATTATTCTTATAGAAGTTCCTACTATTCCAGCTCATGCTCCAAATATAAAGTATAAGGTACCAATATCTT T,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/tempImage5cRch2-scaled.jpg,",41.7760392565,-88.0955267579,09/02/2025,"
Found in a tall grass area, near the trees.<\/p>\n",Orthoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-18-at-10.11.39 AM.png,","",No,>87-CA_Y1_CoR (584 bp) AGTATTCCATTTCGACAGTTAATAATATCGTAATCGCTTCGCTAATACAGGAAGCGAAATAATAAAAGGCGCGCCGTAAA GCTAACCGATCGACAGTAATGGCGTTGGTCAGTGTTACTCCCTTCGATCGTACACAATCGCTGTTGTAACAAAACCGACC GCCCCTAGAGTTGATGAAACACCAGCTAAGTGAAGCGAGAAAATGGCTAAATCAACTGACGCTCCCCATGTGCAATCGCT CCAGCGAGTGGGGGGTAAACTGTTCATCCTGTACCAGCTCCATTATCAACCATAGATGAGGTAAGTAAAAGGGTTAGTGA TGGTGGTAAAAGTCAAAAACTTATATTATTTATTCGTGGAAAAGCTATATCTGGTGCTCCAATTATTAATGGTACAAGTC AATTACCAAATCCTCCAATTATAATAGGTATTACTATAAAGAAAATTATTACAAATGCGTGGGCTGTAATAATTACATTA TAGATTTGGTCATCTCCAATTAGAGATCCTGGTTGTCCTAGTTCTGCTCGAATAATTATTCTTATAGAAGTTCCTACTAT TCCAGCTCATGCTCCAAATATAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/87-CA_Y1_CoR-chrisrrrr.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/D3744969-C4E5-4CC3-988D-F81CC14AFF7B-scaled.jpeg,",41.7762261203,-88.0955535773,09/02/2025,"
found in tall grass area, in the shade<\/p>\n",Melanoplus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_6641.png,","",No,>1-CA_B1_CoR.ab1 (595 bp) TTAATAGCTCCTGCTAATACAGGAAGTGATAATAATAAAAGGAGTGCTGTAATAGCTACTGATCAAACAAATAATGGTGT TTGGTCTAGTGTTATTCTTTCTGATCGTATATTAATTGCTGTTGTAATAAAATTGACTGCCCCTAGAATTGATGAAACAC CAGCTAAGTGAAGAGAGAAAATGGCTAAATCAATTGACGCTCCACCATGTGCAATTGCTCCAGCGAGTGGGGGGTAAACT GTTCATCCTGTACCAGCTCCATTATCAACCATAGATGAGGTAAGTAAAAGGGTTAGTGATGGTGGTAAAAGTCAAAAACT TATATTATTTATTCGTGGAAAAGCTATATCTGGTGCTCCAATTATTAATGGTACAAGTCAATTACCAAATCCTCCAATTA TAATAGGTATTACTATAAAGAAAATTATTACAAATGCGTGGGCTGTAATAATTACATTATAGATTTGGTCATCTCCAATT AGAGATCCTGGTTGTCCTAGTTCTGCTCGAATAATTATTCTTATAGAAGTTCCTACTATTCCAGCTCATGCTCCAAATAT AAAGTATAAGAGTTTGGATTCGGGCAACCCCCGCC,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/1-CA_B1_CoR-new.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/image_2025-11-18_095700765.png,",42.4616208781,-73.3155319305,09/30/2025,"
my arthropod was a Dark-winged Fungus Gnats its order is diptara its family is Cecidomyiidae the scientific name is Genus Afrotricha, it does have Wolbachia\u00a0<\/span><\/p>\n",Sciaridae,Monarch DNA extraction (NEB),Whole arthropod,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/image_2025-12-17_232024556.png,","
Gel electrophoresis system: Edvotek Gel Electrophoresis
\nBuffer: 1X TAE
\nDNA Stain: SYBR Safe<\/p>\n
My first gel Image was taken on 10-21-25 and was run at 125 Volts for 30 minutes Using New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S). This round told me that my arthropod tested false for Wolbachia<\/p>\n
Our second PCR reaction was set up on 10-28-25, used the same Taq polymerase as the first PCR reaction, and:<\/p>\n
was a Single.
\nWe used both the Arthropod CO1 and Wolbachia 16S primer(s).<\/p>\n
while using an annealing temperature of 55 degrees Celsius.<\/p>\n
Second Round:<\/p>\n
Gel electrophoresis system: Edvotek Gel Electrophoresis<\/p>\n
Buffer: 1X TAE<\/p>\n
DNA Stain: SYBR Safe<\/p>\n
Protocol notes: ADD the following statements:<\/p>\n
Our second Gel image was taken on 11-4-25 and:
\nwas run at 125 volts for 30 minutes
\nused this DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S)
\nResults: I am Highly positive that my arthropod is\u00a0 Wolbachia infested, the reason for this is because after running the single Twice to check for any errors It came out with the same results witch leads me to Say in confidence that my arthropod test positively<\/p>\n
This week we did DNA sequencing. For our arthropods only had the ZK-1 Come out due to the CD-1 arthropod being too\u00a0distorted and messy. $\"\"$ CO1: Sequence $\"\"$ S16: Sequence\u00a0 $\"\"$ <\/p>\n",Unknown,AGCTTGAGCTGGAATAGTTGGAACATCTTTAAGAATTTTAATTCGAGCTGAATTAGGACATCCTGGAGCATTAATTGGAGATGATCAAATTTATAATGTAATTGTAACTGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGTGGATTTGGAAATTGATTAGTGCCTTTAATATTAGGTGCTCCTGATATAGCATTCCCACGAATAAATAATATAAGATTTTGACTTCTACCTCCTGCTCTTTCTTTACTATTAGTAAGTAGAATAGTTGAAAATGGAGCTGGGACAGGATGAACTGTTTATCCACCTCTATCCGCTGGAATTGCTCATGGTGGAGCTTCAGTTGATTTAGCTATTTTTTCTCTACATTTAGCAGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACAACTGTAATTAATATACGATCAACAGGAATTTCATTAGATCGTATACCTTTATTTGTTTGATCAGTAGTTATTACTGCTTTATTATTATTATTATCACTTCCAGTACTAGCAGGAGCTATTACTATATTATTAACAGATCGAAATTTAAATACATCATTTTTTGACCCAGCGGGAGGAGGAGATCCTATTTTATACCAACATTTATTTTGA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/stinkbug-image.jpg,",33.9872000000,-84.4397000000,11/07/2025,"
Brown body, White underside, small wings, 2 antannae, 6 legs. Quite passive in the jar.<\/p>\n",Halyomorpha halys,DNeasy (Qiagen) blood and tissue kit.,Abdomen,,,,"","
Isolated lower abdomen of organism by cutting with scalpel<\/p>\n
Extracted DNA and preserved for PCR usage.<\/p>\n",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Ladybug-IMage.jpg,",33.9288000000,-84.4348000000,,"
Black spots on red\/orange body, has tiny wings. 6 legs. Very passive in jar, tiny wings. White spots near eyes.<\/p>\n",Coccinella septempunctata,DNeasy (Qiagen) blood and tissue kit.,Abdomen,,,,"","
Isolated lower abdomen of organism by cutting with scalpel<\/p>\n
Extracted DNA and preserved for PCR usage.<\/p>\n",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Pill-Bug.jpeg,",33.9872670000,-84.4397070000,11/07/2025,"
Pill Bug. Slow movements under the pinestraw. Hard exoskeleton. Gray-Black on top and white on bottom.<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen) blood and tissue kit,Abdomen,,,,"","
\n
Got DNA from the abdomen of the pillbug.<\/li>\n
Finished extracting DNA, now need to conduct PCR.<\/li>\n<\/ul>\n",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Sylvian-Jumping-Spider.jpeg,",33.9330550000,-84.4350930000,11/13/2025,"
Very small. Has a colored dot on its back. Looks like a baby spider.<\/p>\n",Colonus sylvanus,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","
\n
Arthropod was small, so had to use entire body for DNA extraction.<\/li>\n
Extracted the DNA, now need to conduct PCR.<\/li>\n<\/ul>\n",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3080-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3075-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3070-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3068-scaled.jpeg,",38.9443344000,-77.0416243000,08/15/2025,"
Caught outside on a driveway in a fairly wooded, suburban area. Caught in late summer. Weather was cloudy, 89 degrees, with 63% humidity. It seemed old and was less squirmy than the other cicada. In comparison, its colors were more muted and less shiny.<\/p>\n
Top wing on each side is tall and somewhat narrow, standing straight up from the side of the thorax. Bottom wing is shorter and longer, sitting against the side of the abdomen. All wings are very thin. Wings are translucent with strong green and yellow veins. Some edges of the wings are veined, with an especially thick vein on the bottom edge of the bottom wing. It has 4 wings and 6 legs. The foreleg has a thick ovular upper part and a much thinner lower part of the leg. The lower part of the foreleg is bent towards the abdomen. The hind legs has a thicker cylindrical upper part of the leg and thinner lower part that has small hairs. Hind legs appear longer than forelegs. The head is flat with a rounded, triangular face. The mouth is in the center of the face and takes up most of it. It is ridged and roughly rectangular. It has two eyes that are large and spherical. They are facing forward on either side of the face and protrude significantly. It has no antennae. The body has three regions and is roughly cylindrical with a smaller head. The abdomen is thickest closest to the thorax and the tapers off significantly. The top is mostly black with patches of green on the thorax and head. The bottom is brown and black. The legs and wing veins are green and brown.<\/p>\n",Hemiptera,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0730.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0729.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0740.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0739.jpeg,","
DNA Extraction: went as planned<\/p>\n
PCR: went as planned<\/p>\n
Gel electrophoresis, Arthropod CO1: gel #1 lanes – ladder, C-1, C-2, C-3, C-4, positive Wolbachia control, negative Wolbachia control, water control; gel #2 lanes – ladder, C-5, C-6, C-6 again, Wolbachia positive control, Wolbachia negative control, water control<\/p>\n
We accidentally added C-6 DNA to two wells. All of the samples and the arthropod controls had DNA, but the water control did not.<\/p>\n
Gel electrophoresis, Wolbachia 16S: gel #1 lanes – ladder, C-1, C-2, C-3, C-4, positive Wolbachia control, negative Wolbachia control, water control; gel #2 lanes – ladder, C-5, C-6 again, Wolbachia positive control, Wolbachia negative control, water control<\/p>\n
We only added 3 microliters of W- on gel 2. Both W+ had Wolbachia DNA as did C-3.<\/p>\n
DNA sequencing: Sequencing did not work. We are assuming it its genus is Neotibicin based on morphology.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3098-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3093-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3089-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3087-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3088-scaled.jpeg,",38.9443492000,-77.0496646000,07/10/2025,"
Caught near creek in wooded area. Caught in summer. Temperature was 79 degrees. It was found next to its carapice and is greyish-black.<\/p>\n
Wings are long and narrow, widening towards the back of the arthropod. They are also very thin. They extend past the back of the arthropod and sit against the abdomen. Wings are translucent with strong green and yellow veins. The edges of the wings are veined, with especially thick veins on the bottom edges. It has 2 wings and 6 legs. The foreleg has a thick ovular upper part and a much thinner lower part of the leg. The hind legs has a thicker cylindrical upper part of the leg and thinner lower part that has small hairs. All the legs are bent inward. The head is flat with a rounded, triangular face. The mouth is in the center of the face and takes up most of it. It is ridged and roughly rectangular, but narrows away from the eyes. It has two eyes that are large and spherical. They are facing forward on either side of the face and protrude significantly. It has no antennae. The body has three regions and is roughly cylindrical with a smaller head. The abdomen is thickest closest to the thorax and the tapers off significantly. The top is mostly black with patches of brown. The bottom is brown and black. The legs are green and brown and the wing veins are green.<\/p>\n",Neotibicen lyricen,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0739.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0740.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0729.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0730.jpeg,","
DNA extraction: we had to remove liquid from the tube in order to add buffers<\/p>\n
PCR: went as planned<\/p>\n
Gel electrophoresis, Arthropod CO1: gel #1 lanes – ladder, C-1, C-2, C-3, C-4, positive Wolbachia control, negative Wolbachia control, water control; gel #2 lanes – ladder, C-5, C-6, C-6 again, Wolbachia positive control, Wolbachia negative control, water control<\/p>\n
We accidentally added C-6 DNA to two wells. All of the samples and the arthropod controls had DNA, but the water control did not.<\/p>\n
Gel electrophoresis, Wolbachia 16S: gel #1 lanes – ladder, C-1, C-2, C-3, C-4, positive Wolbachia control, negative Wolbachia control, water control; gel #2 lanes – ladder, C-5, C-6 again, Wolbachia positive control, Wolbachia negative control, water control<\/p>\n
We only added 3 microliters of W- on gel 2. Both W+ had Wolbachia DNA as did C-3<\/p>\n
DNA sequencing: Sequence was pretty good. We saved all bases with Q scores > 30.<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/C2CO1-CO1F_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3107-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3107-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3106-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3101-1-scaled.jpeg,",38.9443344000,-77.0416243000,08/15/2025,"
Caught indoors in a wooded area. It was sunny and 78 degrees. 6 legs, no visible wings, oval abdomen, thin antennae.<\/p>\n
2 wings seen under microscope. They are semi-circular and sit against the abdomen. The wings are pretty small and do not extend past the end of the abdomen. It has 6 legs. The forelegs are cylindrical, with a slightly thicker upper part. The upper parts appear to have small hairs. The forelegs have several joints and are splayed outwards. The hind legs have much thicker upper parts and thinner lower parts. The lower parts of the hind legs have small spikes and the upper part has small hairs. It has a circular head with a curved face. The mouth is in the center of the face and is roughly rectangular. It has 2 medium-sized circular eyes, one on each side of the head. The eyes taper slightly towards the thorax and protrude from the head. It has 2 very long, thin antennae. Antennae are straight and extend to either side of the arthropod. It has 3 body regions. The body is roughly cylindrical with a larger abdomen that tapers slightly and a rounded face. It appears to have some sort of tail-like appendage. The top is mostly black with brown patches. The bottom is pale yellow. Legs are pale yellow with brown patches.<\/p>\n",Velarifictorus micado,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0739.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0740.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0729.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0730.jpeg,","
DNA Extraction: We added twice the listed amount of ATL and 10 extra microliters of Proteinase K. We had to remove liquid from C-3 and use an additional filter and several rounds of additional centrifuging to extract the DNA.<\/p>\n
PCR: We recombined the separate tubes from DNA extraction before starting PCR.<\/p>\n
Gel electrophoresis, Arthropod CO1: gel #1 lanes – ladder, C-1, C-2, C-3, C-4, positive Wolbachia control, negative Wolbachia control, water control; gel #2 lanes – ladder, C-5, C-6, C-6 again, Wolbachia positive control, Wolbachia negative control, water control<\/p>\n
We accidentally added C-6 DNA to two wells. All of the samples and the arthropod controls had DNA, but the water control did not.<\/p>\n
Gel electrophoresis, Wolbachia 16S: gel #1 lanes – ladder, C-1, C-2, C-3, C-4, positive Wolbachia control, negative Wolbachia control, water control; gel #2 lanes – ladder, C-5, C-6 again, Wolbachia positive control, Wolbachia negative control, water control<\/span><\/p>\n
We only added 3 microliters of W- on gel 2. Both W+ had Wolbachia DNA as did C-3.<\/span><\/p>\n
DNA sequencing: Both sequences were really good. We saved all bases with Q scores > 30.<\/p>\n",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/C3CO1-CO1F_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_4421.jpeg,",33.9784800000,-84.4787900000,11/09/2025,"
\n
\n
The insect is very active.<\/p>\n<\/li>\n
\n
It was actively trying to climb out of the container.<\/p>\n<\/li>\n
\n
Its wing covers are bright red.<\/p>\n<\/li>\n
\n
It has small black spots on its back.<\/p>\n<\/li>\n
\n
It appears to be a medium-sized ladybug.<\/p>\n<\/li>\n<\/ul>\n",Coccinella septempunctata,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-9.49.25 AM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-at-9.59.46 AM.png,",33.9893000000,-84.4405000000,11/13/2025,"
Since this arthropod is a cultured mealworm, it was found in a box that had oats and other nutritious substances\/material. The mealworm was also found among many other mealworms and super worms. The arthropod moves very slowly with the front end of the arthropod moving followed by the behind. The arthropod moves on its 6 very small appendages at the front of the body. When in the collection container and agitated the arthropod thrashes around slowly. The arthropod has a very uniform shape with a tannish, beige coloration. There are 6 small appendages on the posterior first 1\/4 length of the body. The front of the arthropod has a brownish color that is flat in shape. The arthropod also has multiple scale like partitionings on the anterior side of the body.<\/p>\n",Tenebrio molitor,DNeasy (Qiagen) blood and tissue kit,Abdomen,,,,"","
Remove the abdomen from the mealworm which is the last 3\/4 of the mealworm. Place the abdomen in the microcentrifuge tube, add in the buffer and grind vigorously with the pestle until the body is in the solution. Add in the other buffers step by step to perform the purification. When DNA is purified after being poured into column, elute the column.<\/p>\n",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_9803-e1764862501674.jpeg,",33.9756700000,-84.4066500000,11/09/2025,"
\n
Found on the ceiling walking near a light stimuli (lamp)<\/li>\n
Energetic<\/li>\n
Flys around<\/li>\n
Dark red with small circular black spots<\/li>\n
Oval shaped<\/li>\n
Six legs<\/li>\n<\/ul>\n",Harmonia axyridis,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-13-at-10.57.41 PM.png,",33.9882100000,-84.4386000000,11/10/2025,"
The arthropod was found under a pot in the garden where it was attempting to burrow in damp, moist soil. The arthropod was found deep in the soil, not on the surface. The arthropod was moving very quickly on its appendage when trying to burrow in the soil. When the arthropod was in the collection container, it tried to hide in the dirt in the container. When agitating the container the arthropod moved around very quickly and even tried climbing up the walls. The arthropod has a rod like shape from the thorax to abdomen that is primarily black\/dark brown with red coloration around the end of the abdomen. The head of the arthropod has antennae, while the end of it has a red, prong like structure. The thorax of the arthropod has 3 thin appendages one each side of the body.<\/p>\n",Dermaptera,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-04-102750.png,",33.9756700000,-84.4066700000,11/09/2025,"
\n
Active<\/li>\n
Jumped into container<\/li>\n
Walks a lot<\/li>\n
6 legs- 3 on each side<\/li>\n
Brown with a beige pattern<\/li>\n<\/ul>\n",Halyomorpha halys,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_4422.jpeg,",33.9879100000,-84.4382000000,11/07/2025,"
\n
\n
It was found beneath the soil.<\/p>\n<\/li>\n
\n
It demonstrated low-resistance behavior when handled.<\/p>\n<\/li>\n
\n
The body is brown.<\/p>\n<\/li>\n
\n
It has ringed patterning.<\/p>\n<\/li>\n
\n
The body is thin and elongated.<\/p>\n<\/li>\n<\/ul>\n",Ophyiulus pilosus,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_4320-scaled.jpeg,",36.1444896000,-86.8011462000,10/15/2025,"
small beetle, red stripe<\/p>\n",Chilopoda,pcr,Abdomen,MiniPCR,lysis,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_4733-scaled.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_4321-scaled.jpeg,",36.1444896000,-86.8011462000,10/15/2025,"
Fire ant<\/p>\n
<\/p>\n",Arthropoda,pcr,Abdomen,MiniPCR,lysis,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_4733-scaled.jpeg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_1882.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_1881.jpg,",,,,"
\n
Date of collection: 8\/28\/2025<\/span><\/li>\n
Time of collection: 10:50 am<\/span><\/li>\n
Location of collection: Near Lane Library and Gamble Hall<\/span><\/li>\n
Description of the environment (habitat) of collection site: Caught in bushed, near trees, on the grassy area where there’s sticks, leaves, and other insects<\/span><\/li>\n
Method of collection: Aerial Net, gloves, glass jar, collection tray, and phone to take pictures<\/span><\/li>\n<\/ul>\n",Diptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_1657-scaled.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,TGGAACATCTTTAAGAATTTTAATTCGAGCTGAATtattacATCCTGGAGCATTAATTGGAGATGATCAAATTTATAATGTAATTGTAACTGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGTGGATTTGGAAATTGATTAGTGCCTTTAATATTAGGTGCTCCTGATATAGCATTCCCACGAATAAATAATATAAGATTTTGACTTCTACCTCCTGCTCTTTCTTTACTATTAGTAAGTAGAATAGTTGAAAATGGAGCTGGGACAGGATGAACTGTTTATCCACCTCTATCCGCTGGAATTGCTCATGGTGGAGCTTCAGTTGATTTAGCTATTTTTTCTCTACATTTAGCAGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACAACTGTAATTAATATACGATCAACAGGAATTTCATTAGATCGTATACCTTTATTTGTTTGATCAGTAGTTATTACTGCTTTATTATTATTATTATCACTTCCAGTACTAGCAGGAGCTATTACTATATTATTAACAGATCGAAATTTAAATACATCATTTTTTGACCCAGCGGGAGGAGGAGATCCTATTTTATACCAACATTTATTTTGATTTTTTGGTCA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_2755-1-scaled.jpg,",31.9708128,-81.15865149999999,08/28/2025,"
Collected by a sweep net in a grassy field at Georgia southern university’s Armstrong campus.<\/p>\n",Blattodea,,body without wings,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-13-110834.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_9376-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_9375-1.jpeg,",33.9892669,-84.440469,11/07/2025,"
The arthropod was buried, moving under a pile of leaves near a creek. The arthropod resembled a spider and had as many legs and was brown in color.<\/p>\n",Chelifer cancroides,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","
\n
Involved grinding the arthropods to ensure a high DNA yield<\/li>\n<\/ul>\n",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_9373-3-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/IMG_9374-2.jpeg,",33.9892669,-84.440469,11/07/2025,"
The arthropod was small in size, approximately 2-3 millimeters in length. The arthropod was moving with 6-8 legs and was brown in body color.<\/p>\n",Dermacentor variabilis,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","
Involved grinding the arthropods to ensure a high DNA yield<\/p>\n",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_7370-scaled-e1763008923104.jpeg,",31.9708128,-81.15865149999999,08/25/2025,"
Caught in grassy field, few shrubs\/trees, some dead grass.
\nCaught using sweep net.<\/p>\n",Odonata,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-12-at-11.52.59 PM.png,","
A DNA extraction kit of in-house reagents was used.<\/p>\n",No,TAGTAGGTACAGCGCTGAGAGTTTTAATTCGGATTGAATTAGGTCAGCCA GGGTCTCTAATTGGTGATGATCAAATTTATAATGTAATTGTAACTGCCCA CGCTTTTGTAATAATTTTTTTTATAGTTATACCTATCATAATTGGAGGTT TTGGTAACTGACTAGTACCTTTAATATTAGGAGCTCCTGATATGGCCTTC CCACGATTGAATAATATAAGTTTTTGATTATTACCTCCTTCCTTTACACT ACTTTTAGCTAGAAGTATGGTAGAAAGTGGTGCAGGAACAGGATGAACAG TTTATCCCCCATTGGCAGGAGCTATTGCCCATGCTGGTGCATCTGTTGAC TTAACCATTTTTTCATTGCATTTAGCTGGTGTGTCATCAATTTTAGGGGC CATTAATTTTATTACAACAGTAATCAATATAAAATCCCCTGGAATAAGTA TTGATCAACTACCATTATTTGTATGGGCAGTAGTAATCACTGCAGTATTA CTTCTTTTATCATTACCAGTACTAGCTGGTGCTATTACCATGCTGTTAAC TGATCGTAATATTAATACATCTTTCTTTGACCCAGCAGGAGGTGGAGACC CAatcTTATACCAACACTTATTTTGATTTTTTGGTCA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/image0-3-rotated.jpeg,",31.9793494784,-81.1608502402,08/28/2025,"
Was caught in grassy\/dirt areas.<\/p>\n",Supella longipalpa,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Picture1.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_3939-scaled.jpg,",31.9708128000,-81.1586515000,08/27/2025,"
Sucking mouth part, Brown, antennas<\/p>\n",Hemiptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/2261785008451181699.jpg,","
DNA extraction kit of in-house reagents was used<\/p>\n",Yes,ATCTTCAATAAGATGAATTATCCGAATTGAATTAGGACAACccgtaacgt ttATTGGAAATGATCAAATTTATAATGTTATTGTTACtgtacATGCATTT ATTATAATTTTTTTCATAGTTATACCAATCATAATTGGAGGATTTGGAAA TTGATTAATCCCTTTAATAATTGGAGCCCCTGATATAGCATTCCCCCGAA TAAATAATATAAGATTTTGATTATTACCCCCCTCATTGACTTTATTATTA ATAAGAAGAATAGTAGAAATAGGAGCAGGAACAGGATGAACTGTATACCC TCCTTTATCTAATTCTATATTTCACAGAGGAGCCCCAGTAGATATAGCAA TTTTTTCTTTACATTTAGCAGGTATTTCATCAATCTTAGGAGCTATTAAT TTCATTTCAACAATTATTAATATACGACCATCAGGTATAACTCCTGAAAA GACACCACTATTTGTATGATCAGTTGGTATTACTGCTCTATTACTATTAT TATCATTACCAGTATTAGCTGGAGCCATCACTATACTATTAACCGACCGA AATTTCAATACTTCATTTTTTGATCCAACAGGAGGAGGAGACCCTATTCT ATATCAACATCTATTTTGATTTTTTGGTC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/unnamed-4-scaled.jpg,",31.9708128,-81.15865149999999,08/27/2025,"",Blattidae,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_5150-scaled.jpeg,","
dna extraction kit of in-house reagents was used<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0307-scaled.jpeg,",36.1444896000,-86.8011462000,,"
$\"\"$ <\/p>\n",Arthropoda,,Partial abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0250-scaled.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250910_224502912-1-scaled.jpg,",31.9858113000,-81.0865035000,08/30/2025,"
Beat net and gloved hands methodology<\/p>\n",Diptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Gel-1.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",Yes,CATGAGCAGGAATAGTTGGAACTTCACTAAGTATATTAATTCGAACAGAA TTAAGCCATcccgtttttgttaTTGgngATGATCAAATTTATAATGTAAT TGTtactgtccaTGCTTTTATTATAATTTTTTTTATAGTTATACCAATTA TAATTGGTGGATTTGGAAACTGATTAGTTCCATTAATATTAGGAGCCCCT GATATAGCTTTTCCTCGAATAAATAATATAAGATTTTGACTTTTACCACC TTCTTTATCCCTTCTTCTAATAAGAAGCATAGTTGAAAACGGGGCTGGTA CTGGATGAACAGTATATCCCCCTCTTTCTAGAAATATTGCCCATGCAGGA GCTTCTGTTGATTTAGCAATTTTTTCTCTTCATTTAGCTGGTATTTCTTC TATTTTAGGGGCTGTTAATTTTATTACTACAGTTATTAATATGCGATCTA CTGGAATTACATTTGATCGTATACCACTTTTTGTATGATCTGTAGTTATT ACAGCCGTTTTACTTCTCCTATCCCTCCCAGTTTTAGCCGGGGCTATTAC TATACTATTAACAGATCGAAATTTAAATACAAGTTTCTTTGACCCAGCAG GAGGGGGAGACCCTATTTTATACCAACATTTATTTTGATTTTTTGGTCAC CCTGAAGTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_3044-scaled.jpeg,",,,08/27/2025,"",Blattodea,,Abdomen,Standard electrophoresis system,1X TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0056.jpeg,","",Unknown,TGGATAGTAGGGACATCTTTAAGAATATTAATCCGAGCAGAACTAAATCA ACCtgttTCATTAATTGGAGATGATCAAATCTATAATGTTATTGTAACGG CTCACGCTTTTGTTATAATTTTCTTTATAGTTATGCCAATTTTAATTGGA GGATTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGC ATTTCCTCGTATAAATAATATAAGTTTTTGACTTTTACCCCCATCTCTAT CTCTTCTATTAGCCAGTAGCCTTGTCGAAAGAGGAGCTGGGACTGGTTGA ACCGTATACCCTCCCTTAGCTAGAGGAATTGCTCATGCTGGAGCTTCAGT AGATTTAGCTATTTTCTCATTACATCTTGCAGGTGTATCATCAATTTTAG GTGCCGTAAATTTTATTTCAACAATTATTAACATGAAACCAATTAATATA AGCCCTGAACGAATTCCTTTATTTGTTTGGTCAGTAGGTATTACTGCATT ATTATTATTATTATCCTTACCAGTTCTTGCAGGTGCAATTACAATATTAT TAACAGACCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGGGGAGGT GATCCTATTTTATATCAACATTTATTTTGATTTTTTGGTCA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_8687-scaled.jpeg,",,,08/27/2025,"
Used a sweep net outside on trees on campus.<\/p>\n",Hymenoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-07-at-11.50.56-PM.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_8693-scaled.jpeg,",,,08/27/2025,"
Collected outside of campus buildings under shaded trees using a sweep net.<\/p>\n",Orthoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-07-at-11.50.56-PM.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_1043-scaled.jpeg,",31.9708128,-81.15865149999999,08/27/2025,"",Dermaptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_3323-scaled.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_8654-1-scaled.jpeg,",31.9708128000,-81.1586515000,08/27/2025,"
Collected in long grass by the aquaponics center<\/p>\n
Used a swoop net and a jar<\/p>\n",Arthropoda,,head and lower body,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_8784-scaled.jpg,","
A DNA extraction kit of in-house reagents was used<\/p>\n",Yes,GAGCTTGATCTGCTATAGTAGGAACTTCTTTAAGAATTTTAATTCGAACA GAATTAGGTCAACccgtatcattaATTGGAGATGATCAAATTTATAACGT AATTGTTACTGCTCACGCTTTTGTAATAATTTTTTTTATAGTAATACCAA TTATAATTGGTGGATTTGGAAATTGATTAGTTCCTTTAATATTAAGAGCT CCTGATATAGCTTTCCCTCGAATAAATAATATAAGATTTTGATTATTACC ACCTTCTTTAACTCTCCTATTATCCAGAAGATTAGTAGAAAACGGTGCAG GAACCGGATGAACAGTGTACCCTCCACTATCATCTCTAATTGCCCACAGA GGTGCATCCGTTGATCTTGCAATTTTTTCTCTTCATTTAGCTGGAATTTC ATCAATTTTAGGAGCAATTAATTTTATTTCTACTATAATTAATATACGAG CTCCAGGTATAACTTTAGATCAAACTCCTTTATTTGTATGAGCTGTAGGT ATTACAGCCCTTCTTCTTCTTTTATCTCTTCCAGTTCTAGCAGGAGCTAT TACTATACTTTTAACAGATCGTAATTTAAATACTTCTTTTTTTGATCCTG CTGGAGGAGGAgatCCTATTTTATATCAACACTTATTTTGATTTTTTG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_1524-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_1523-scaled.jpeg,",31.9708128,-81.15865149999999,08/17/2025,"
abandon garden, very grass area, fenced off<\/p>\n",Coleoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_1806.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Untitled133_20250913180443.jpeg,",31.9708128,-81.15865149999999,08/27/2025,"
\n
Collected in short grass behind the greenhouse near the pond on campus<\/li>\n
Used gloved hands and a specimen jar to collect<\/li>\n<\/ul>\n",Hemiptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0051.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,GAGCAGGTATAGTAGGATCCGCTATAAGACTAATTATCCGAATCGAACTA GGACAACCaggtagATTCATTGGGGATGATCAAATTTATAACGTAATCGT AACAGCTCACGCCTTCGTAATAATTTTCTTCATAGTAATACCTATTATAA TTGGAGGATTTGGAAACTGACTAGTTCCCCTAATAATCGGAGCTCCCGAC ATAGCATTCCCTCGTATAAATAACATAAGATTTTGATTATTACCTCCTTC ATTAACATTATTAATAATAAGTAGACTAGCAGAATCAGGAGCTGGAACCG GATGAACAGTTTACCCTCCTTTATCAAGTAATTTATCTCACAGAGGAGCA TCCGTAGACTTAGCAATTTTTTCACTCCACTTAGCCGGGGTATCTTCAAT CTTGGGAGCAGTAAATTTTATTTCCACAATCATTAATATACGACCCGCAG GAATAATCCCTGAACGAATTCCTTTATTTGTATGATCGGTAGGGATTACT GCCTTACTTCTACTACTATCATTACCTGTGCTAGCAGGTGCTATTACAAT ATTATTAACTGATCGAAATTTTAACACATCATTTTTTGATCCCTCAGGTG GAGGAGACCCTATTTTATACCAACATTTATTTTGATTTTTTGGTCA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Ant-diagram-scaled.jpg,",31.9708128,-81.15865149999999,08/28/2025,"
Caught between concrete, with dirt in between the concrete.<\/p>\n",Hymenoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Gel-3-scaled.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Ant-drawing-scaled.jpg,",,,08/27/2025,"
Collection Method- Gloves<\/p>\n
Detailed description of the collection site habitat- Planted tree, bushes, pine straw<\/p>\n
<\/p>\n",Hymenoptera,,head and lower body,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/labeled-gel-correct.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,ATCATCTATAAGAATAATCATCCGATTAGAACTAGGATCTTGTAATTCTC TAATTAATAATGATCAAATCTACAACTCCTTAGTTACTAGACACGCTTTT ATTATAATTTTTTTTATAGTTATGCCCTTCATAATTGGAGGATTTGGAAA TTTCTTAGTGCCCTTAATACTTGGGTCCCCCGATATGGCCTATCCTCGAA TAAATAATATAAGATTTTGACTTCTGCCACCTTCTCTAACTCTTTTACTT ATCAGAAGATTTATTAACAGAGGAGTAGGAACAGGATGAACTATCTACCC ACCATTAGCCTCCAATATTTTTCACAGAGGGGCCTCTATTGATCTATCTA TTTTTTCCTTACATATCGCCGGAATATCATCAATTTTAGGAGCTATTAAT TTTATTTCTACAATCATTAACATACACCATAAAAATTTTACTATAGATAA AATCCCCCTACTAGTTTGATCTATCCTTATTACAGCCATTCTTCTTTTAC TTTCACTCCCAGTTCTTGCAGGAGCTATTACAATATTACTAACTGACCGT AATTTAAACACCTCCTTCTTCGATCCCTCAGGAGGAGGAGATCCCATTCT ATACCAACATTTATTCTGATTTTTTGGTCACC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0888-scaled.jpg,",,,08/27/2025,"",Hemiptera,pcr,,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_1294.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/fly-drawing-1-scaled.jpg,",31.9708128,-81.15865149999999,08/27/2025,"
2 wings, 6 legs, 3 body parts, 2 antennae<\/p>\n",Hymenoptera,,head and lower body,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Gel-Electrophoresis-Results-2.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,TTTTTGCATATGATCAggaatAaTTGGATCTTCCTTAAGAATAATTATTC GAATAGAATTAAGAATTCccgtatCATGAATTAGTAATGACCAAATTTAT AATTCCTTTATTACattgcACGCATTTTTAATAATTTTCTTTTTAGTTAT ACCGTTTTTAATTGGTGGATTTGGAAATTGACTCACACCTTTAATATTAG GAGCCCCTGATATAGCTTTCCCCCGTATAAATAATATTAGATTTTGATTA TTACCCCCCTCATTATTAATATTATTATCAAGAAATTTAATTAACCCAAG ACCTGGAACAGGATGAACAGTTTATCCACCACTATCTTCTTATACTTATC ATCCTTCTCCTTCTGTAGATCTAGCAATTTTTTCTTTACACTTATCAGGT ATTTCTTCTATTATTGGATCAATAAATTTTATTGTAACAATTTTATTAAT AAAAAATTATTCAATAAATTATAATCAAATACCTTTATTTCCCTGATCTG TTTTAATTACAACAATTTTATTACTATTATCACTACCTGTATTAGCAGGA GCAATTACAATATTATTATTTGATCGAAATTTAAATTCATCATTTTTCGA CCCTATAGGAGGAGGAGATCCAATTTTATATCAACATTTATTTTGATTTT TTGGTCACCctgAAGTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Picture1.jpg,",,,08/20/2025,"
-08\/20\/2025 :Wednesday \u20132:46 pm1<\/p>\n
Location<\/b>1<\/b><\/p>\n
–\u00a0Georgia Southern Armstrong Pond 1<\/p>\n
Habitat <\/b>1<\/b><\/p>\n
-Low tide pond1<\/p>\n
Method of Collection: <\/b>1<\/b><\/p>\n
-Dip Net 1<\/p>\n
Method of Viewing :<\/b>1<\/b><\/p>\n
-Dissecting Magnification Glass1<\/p>\n
–<\/p>\n",Insecta,,Abdomen,Standard electrophoresis system,1X TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Picture2.png,","
DNA extraction kit of in house raegents used<\/p>\n",No,,,, "",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/tempImageI2TZb9-scaled.jpg,",,,08/27/2025,"
time: 1:40pm<\/p>\n
Georgia Southern University Armstrong campus in the field near the greenhouse<\/p>\n
caught with aerial net<\/p>\n",Orthoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/tempImagesxUWMt-scaled.jpg,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,GTAGGCACATCATTAAGTATTTTAATTCGAACAGAACTAGGACAACCAGGCTATTTAATTGGAGATGATCAAACTTATAATGTTATCGTAACCGCACATGCATTTATCATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGATTAGTACCATTAATATTGGGGGCTCCAGATATAGCCTTCCCACGAATAAACAATATAAGATTTTGACTTCTACCCCCATCATTAACCCTTTTATTAACCAGAAGAATAGTTGAAAATGGTGCAGGAACAGGATGAACAGTATACCCACCTCTATCAACAGGAATCGCTCACGCGGGAGCATCCGTCGATTTAGCTATCTTCTCACTACATTTAGCAGGAATTTCCTCAATTCTAGGGGCTGTAAATTTTATCACTACCATAATTAATATACGAGCTCCAGGAATATCACTAGACCAAACACCATTATTTGTTTGAGCAGTCGGAATTACAGCTCTTCTATTATTATTATCCTTACCAGTTTTAGCTGGTGCTATTACAATATTGCTTACAGATCGAAATTTAAATACATCATTTTTTGATCCAGCGGGAGGAGGGGATCCAATCTTATATCAACATCTATTTTGATTTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/tempImageJmVijt-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/tempImageCFkJzz-scaled.jpg,",,,08/27/2025,"
Unkept Grassy feild with tall grass.<\/p>\n
sunny<\/p>\n",Hemiptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/tempImageY1B8FJ-scaled.jpg,","
DNA extraction kit of in-house reagents was used<\/p>\n",Yes,GATCCGCAATAAGTGTTATTATTCGAGTAGAATTAGGACaccctgtagag agttATTGGAGATGACCAAATTTATAATGTTATAGTAactgtacATGCAT TTATTATAATTTTCTTTATAGTGATGCCAATTATAATTGGAGGATTTGGA AATTGATTAGTACCTCTAATAATTGGAGCCCCTGATATAGCATTCCCCCG ACTAAATAATATAAGATTTTGATTATTACCCCCCGCTTTAACCTTACTAT TAATGAGAAGATTAACAGAAAGTGGGGCCGGAACTGGATGAACAGTTTAT CCACCTTTATCAAGTAATTTATCCCACAGAGGAGCATCAGTAGATATAGC TATTTTTAGTTTACATTTAGCTGGAGTATCTTCTATTCTAGGAGCAATAA ACTTTATTTCAACTATCATAAATATGCGCCCAGTAGGAATAACACCAGAA CGTATCCCATTATTTGTATGATCAGTTGGAATTACAGCATTACTTTTACT TTTATCCCTACCCGTCTTAGCAGGAGCCATTACAATACTTTtAactGaTc gaAACTTTAATACATCATTCTTCGATCCcttcaggaGGaggggatCCTGT ACTatacccagctttTATttTgatTtTTtggtcACcctgaaaattaacgc ttcttcatactttagtaacataaacaaactcacacttacatttaaattct acacaacagtaatatacctgtttacaccggtaccttatttttccctatcc aacctcctccataccctcacctttctctaaatcacacccaaaagcacgct aacacgaacactccaaaccccctcacttAtccttcttcactacctcaatc tACtCtcgtatctcacaactcttctcagtagacgatatctattttcaaaa aaAAAAAAAAAAAAAAAAAAAAAAAAAAA,,, "",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_4344.jpeg,",,,08/27/2025,"
long<\/p>\n",Dermaptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-10-31-233742.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,TCGGGgatgGTAGGAACTAATTTGAGAATTTTAATTCGAACAGAGTTAGG TCAGCCTGGATCTTTAATTGGAGATGATCAGATTTATAATGTTATTGTTA CTGCTCACGCTTTTATTATAATTTTTTTTATGGTAATGCCAATTATAATT GGAGGGTTTGGTAATTGATTAGTTCCTTTAATATTGAGAGCTCCAGATAT AGCTTTCCCACGAATGAATAATATAAGATTTTGGTTATTACCTCCATCTC TGATTTTATTATTATCAGGGGGCCTAGTAGATAGAGGGGCTGGGACAGGT TGGACAGTGTATCCTCCTTTATCATCAGTGATTTCTCATGGAGGGGCTTC AGTGGATATAACTATTTTTTCTTTACATTTGGCAGGGGTTTCTTCTATTT TGGGAGCCATTAATTTTATTACTACTGTGATTAATATACGTCCAGAAGGT TTAAAGCTTGATCGTGTTCCTTTATTTGTTTGATCAGTAGCTATTACTGC TTTATTATTATTGCTTTCTTTACCGGTTTTAGCAGGAGCTATTACGATAT TATTAACTGATCGAAATTTAAATACATCTTTTTTTGATCCAGCCGGAGGC GGAGATCCTATTTTATATCAACATTTATTTTGATTTTTTGGTC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/odonata.jpg,",31.9708128000,-81.1586515000,08/27/2025,"
Small green insect.<\/p>\n",Ischnura elegans,,Whole arthropod,Standard electrophoresis system,1X TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/gel-2.jpg,","
N\/A,DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,AGCAGGTATAGTAGGAACTGCTTTAAGAATATTAATTCGAGTTGAACTCG GACAACCagtatcgctAATTGGAGATGATCAAATTTATAATGTAGTAGTA ACttcaCACGCTTTTGTTATAATTTTTTTTATAGTAATACCAATCATAAT TGGTGGGTTCGGAAATTGGTTAGTACCACTAATATTAGGGGCACCAGATA TGGCTTTCCCTCGATTAAATAATATAAGATTTTGACTCTTACCCCCATCT TTAACCCTTCTTCTAGCAAGAAGATTAGTAGAAAGAGGAGCTGGAATTGG TTTCACTcgAAGCTatCCGTtngcngGAGTTATTGCTCATGCTGGAGCGT CAGTAGATTTAACAATTTTTTCATTACATTTAGCGGGAGTATCCTCAATT TTAGGAGCAATTAATTTTATTACAACTACAattnaatATAAAATCACCTG GAATganataTAGATCAATTACCATTGTTTGTATGAGCTGTAGTTATTAC AGCAGTGTTACTTTTATTATCATTACCAGTATTAGCTGGAGCAATTACTA TACTTTTAACTGATCGTAataTTAATACTTCATTTTTTGATCCCGCTGGA GGAGGGGATCCTATCTTATATCAACATTTATTTTGATTTTTTGgtcACCC TgA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_7281.jpg,",31.9708128,-81.15865149999999,08/25/2025,"
\u2022Date: Aug 25, 2025<\/div>\n
\u2022Time: 1:20 PM<\/div>\n
\u2022Location: Outside GSU Science Center<\/div>\n
\u2022Habitat: grassy area with leaf litter & shrubs<\/div>\n
\u2022Method: manual capture-Sweep net<\/div>\n",Diptera,Qiagen DNeasy Blood and Tissue Kit.,,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/AutumnEmilyDiana-scaled.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_3584-1-scaled.jpeg,",31.9708128,-81.15865149999999,08/25/2025,"
\u2022the traits were:<\/p>\n
\u2022stringer<\/p>\n
\u2022 two sets of wings<\/p>\n
\u2022fully developed wings<\/p>\n
\u2022Antennas<\/p>\n
\u2022Mandibles<\/p>\n",Insecta,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_4020-scaled.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,TGAGCAGGAATAATTGGATCATCAATAAGAATAATTATTCGAATTGAATT AGGATCCTGTGGATCCATTATTAATAATGACCAACTGTATAACTCTATCG TAACAGGACATGCATTTATCATAATTTTCTTTATAGTTATACCATTTATA ATCGGAGGATTTGGTAACTTTTTAATTCCACTAATAATTGGGTCACCAGA TATAGCTTACCCCCGTATAAATAACATAAGATTTTGACTCTTACCCCCAT CAATTATACTACTCACCTTAAGAAGATTTATTAATTCAGGTGCGGGAACT GGTTGAACTGTATACCCTCCCTTATCCTCAAGAATTTTCCATAGGGGAGC CTCAGTAGATTTAGCAATTTTTTCTCTCCATATTGCAGGTATTTCATCAA TCATAGGAGCTATCAACTTCATCTCTACAATTATTAACATAACCCATAAA AATCTTTCAATAGATAAAACCCCTCTCATAGTATGATCTATTCTAATTAC AGCAGTCCTTCTCCTCCTTTCCCTTCCTGTATTAGCCGGAGCAATTACAA TACTACTAACAGATCGAAACCTTAATACTTCATTTTTTGACCCAGCAGGG GGAGGAGATCCTATTCTTTACCAACATTTATTTTGATTTTTTGGTC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Upload-from-mobile-1762894608IMG_2692-scaled.jpeg,",,,09/09/2025,"
collection method: aerial sweep nets and gloved hands.<\/p>\n",Periplaneta americana,,In front of University Building,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_2058.jpeg,","
DNA extraction kit of in-house reagents was used<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_4036-scaled.jpeg,",,,08/26/2025,"
Time of collection: Around 3pm<\/span>\u200b<\/p>\n
Location of collection: In between the <\/span>science center and library near the <\/span>parking area on Library Dr<\/span>\u200b<\/p>\n
Environment description: grassy\/near <\/span>shrubs and underneath the area of a <\/span>tree<\/span>\u200b<\/p>\n
Method of collection: sweep net<\/span><\/p>\n",Coleoptera,,Other,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-11-at-3.55.48 PM.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_7280-2-scaled.jpg,",31.97408334801747,-81.15508102297919,08/26/2025,"
Found under dead leaves in dirt. Had pinchers, no wings, and mandibles.<\/p>\n",Dermaptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_5815-1-scaled.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_4706-scaled.jpeg,",31.97938254053995,-81.16096668585833,08/26/2025,"
Small,<\/p>\n",Insecta,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/gel-1-scaled.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n
<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0534-scaled.jpeg,",,,09/16/2025,"
Collected sample in dirt with hands, it was covered by leaves. Insect is light in color.<\/p>\n",Lasius nearcticus,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-11-at-3.46.26 PM.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,GCTTGATCAGGAATGGTCGGAACATCACTCAGAATACTAATTCGAACAGAATTAGGACAACCTTTGTGTG TGATTGGAGACGACCAGATCTACAACGTCATCGTTACCGCCCACGCATTCGTTATAATCTTCTTTATGGT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-11-at-15.07.16.png,",,,08/26/2025,"
Collected 8\/26\/25 at 13:20 <\/span>using sweep net. <\/span>Habitat of short, fresh cut grass with few trees. <\/span>Collected in east-westernmost field between Science Center and Jenkins Hall.<\/span><\/p>\n",Hemiptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-11-at-15.08.29.png,","
A DNA extraction kit of in-house reagents was used.<\/p>\n",No,ATAATCATCCGGGTTGAATTAGCACagccattatcgctATTCTTCTCTAA TAACAGACAAGTTTACAATGTAATTATTACTTCTCATGCATTCATTATAA TTTTCTTTATAGTTATACCCATTATAATTGGAGGTTTCGGAAACTGACTT CTCCCTCTTATAATTGGAGCACCTGATATAGCATTTCCTCGTCTTAATAA TATAAGATTCTGACTTCTACCCCCATCATTATCTCTTTTACTTATAAGAT CTATAATTGAAATAGGAGTGGGAACAGGATGAACTGTTTATCCCCCTTTG TCAAGAAACATTGCCCACGCTGGGCCTAGAGTTGACATATCAATTTTTTC ATTACACTTAGCTGGTATCTCATCAATTCTAGGAGCAGTCAATTTTATTA CGACTGTAATAAACATACGACCTACAGGTTTAACACTTCACCAAACACCA TTATTTGTTTGATCTGTTTTAATTACAGCAGTTCTTCTACTATTATCCCT TCCAGTACTTGCAGGGGCAATTACTATACTTTTAACAGACCGAAACATCA ATACAACATTTTTTGACCCCTCAGGAGGGGGTGACCCTATTCTCTATCAA CATTTATTTTGATTTTTTGGTCACC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0772.jpeg,",36.1444896000,-86.8011462000,09/26/2025,"
\n
Tuesday, August 26th, 2025<\/li>\n<\/ul>\n
\n
Took 45 minutes to collect our specimen (1:00 PM to 1:45)<\/li>\n<\/ul>\n
\n
Science Center of Georgia Southern University Armstrong Campus<\/li>\n<\/ul>\n
\n
Dragonfly in a grassy area with small plants and a few trees near the Student Success Center (an open space with direct exposure to sunlight). Caught mid-air<\/li>\n<\/ul>\n
\n
To collect our insects, we used a:<\/li>\n<\/ul>\n
\n
Sweep Net 9<\/li>\n
Beat Net and Stick 9<\/li>\n
Collection Tray 9<\/li>\n
Large Specimen Jar 9<\/li>\n
Gloves<\/li>\n
Phones<\/li>\n<\/ul>\n",Odonata,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0773.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_2299-scaled.jpeg,",,,08/26/2025,"
Tools Used: Sweep Nets, beating sheets, Specimen Jar, and gloves.\u00a0<\/span><\/p>\n
Outcome: I collected my Mosquito in the long grasses using the collection jar to catch it. My partner, Autumn collected a dragonfly using the collection jar.\u00a0<\/span><\/p>\n

<\/p>\n",Insecta,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-11-at-3.04.13 PM.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_5713-scaled.jpeg,",,,08/26/2025,"
Found perched on a flower near the Learning Commons. Caught with a sweep net.<\/p>\n",Lepidoptera,,body without wings,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/SophiaJaide.jpeg,","
DNA extraction kit of in-house reageants was used.<\/p>\n",No,GTAGGAACATCTCTTAGTATTTTAATTCGAATAGAATTAGGTAATCCCgt aTCATTAATTGGTGATGATCAAATTTATAATACTATTGTTACAGCTCATG CATTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTT GGTAATTGATTAGTTCCATTAATATTAGGAGCCCCAGATATAGCATTTCC TCGAATAAATAATATAAGATTTTGACTTCTCCCCCCGTCATTAATTTTAT TAATTTCTAGAAGAATTGTAGAAAATGGAGCAGGAACAGGATGAACTGTT TATCCACCTCTTTCATCTAATATTGCTCATGGAGGTTCATCTGTAGATTT AGCTATTTTTTCCTTACATTTAGCCGGAATTTCTTCAATTTTAGGGGCAA TTAACTTTATTACTACTATTATTAATATACGAATTAATAATATATCTTTT GATCAATTACCTTTATTTGTTTGAGCTGTAGGAATTACAGCACTTCTTTT ATTATTATCTCTTCCAGTTTTAGCTGGAGCTATTACTATACTTTTAACAG ATCGAAATTTAAATACATCATTTTTTGATCCTGCTGGAGGGGGAGACCCA ATTTTATATCAACATTTATTTTGATTTTTTGGT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_4514-scaled.jpeg,",,,08/26/2025,"
Time of collection: 3:00 p.m. – 3:30 p.m.
\nLocation of collection: Outfront of Gambell Hall and near the fountain.
\nThe environment: Topsoil, stones, pinestraw
\nMethod of collection: By hand<\/p>\n",Insecta,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_1952-scaled.jpeg,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,GTAGGAACTAATTTGAGAATTTTAATTCGAACAGAGTTAGGTcagcCttt atctTTAATTGGAGATGATCAGATTTATAATGTTATTGTTACTGCTCACG CTTTTATTATAATTTTTTTTATGGTAATGCCAATTATAATTGGAGGGTTT GGTAATTGATTAGTTCCTTTAATATTGAGAGCTCCAGATATAGCTTTCCC ACGAATGAATAATATAAGATTTTGGTTATTACCTCCATCTCTGATTTTAT TATTATCAGGGGGCCTAGTAGATAGAGGGGCTGGGACAGGTTGGACAGTG TATCCTCCTTTATCATCAGTGATTTCTCATGGAGGGGCTTCAGTGGATAT AACTATTTTTTCTTTACATTTGGCAGGGGTTTCTTCTATTTTGGGAGCCA TTAATTTTATTACTACTGTGATTAATATACGTCCAGAAGGTTTAAAGCTT GATCGTGTTCCTTTATTTGTTTGATCAGTAGCTATTACTGCTTTATTATT ATTGCTTTCTTTACCGGTTTTAGCAGGAGCTATTACGATATTATTAACTG ATCGAAATTTAAATACATCTTTTTTTGATCcagcCGGAGGCGGAGATCCT ATTTTATATCAACATTTATTTTGattTTTTGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/0D687550-5BD9-4A17-85D1-B34D1560F3C7.jpeg,",,,08/26/2025,"
In the grass with another one of its kind.<\/p>\n
Bright orange thorax<\/p>\n
<\/p>\n",Diptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/A2E809F6-BD7C-4211-857A-7A6A1D1BACCC.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,GTTGGAACTTCACTAAGTATATTAATTCGAACAGAATTAAGCCATccctt ttttggtaTTGGTGATGATCAAATTTATAATGTAATTGTTAcatttcATG CTTTTATTATAATTTTTTTTATAGTTATACCAATTATAATTGGTGGATTT GGAAACTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTTCC TCGAATAAATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTC TTCTAATAAGAAGCATAGTTGAAAACGGGGCTGGTACTGGATGAACAGTA TATCCCCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTT AGCAATTTTTTCTCTTCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTG TTAATTTTATTACTACAGTTATTAATATGCGATCTACTGGAATTACATTT GATCGTATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACT TCTCCTATCCCTCCCAGTTTTAGCCGGGGCTATTACTATACTATTAACAG ATCGAAATTTAAATACAAGTTTCTTTGACCCAGCAGGAGGGGGAGACCCT ATTTTATACCAACATTTATTTTGATTTTTTGGTCAC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_2294-scaled.jpg,",,,08/26/2025,"",Diptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/RebeccaTalia.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,TAGGAACTTCCCTTAGTATTATTGTTCGAATAGAATTAGGTCATCCtgtg gCATTAATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCTCATGC TTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTG GAAATTGACTTGTGCCTTTAATATTAGGAGCCCCTGATATGGCTTTTCCC CGAATAAATAATATAAGATTTTGAATATTACCTCCCTCTTTAACATTATT GTTAGCAAGAAGAATAGTAGAAAATGGGGCAGGAACAGGATGAACAGTTT ATCCACCTCTATCTGCAGGAATTGCTCATGGAGGAGCTTCTGTAGATTTA GCTATTTTTTCATTACATTTAGCAGGTATTTCTTCAATTTTAGGAGCAGT AAATTTTATTACTACAGTTATTAATATACGATCAACAGGTATTACATTTG ATCGAATACCATTATTTGTTTGATCTGTAGTAATTACTGCTATTTTATTA TTACTTTCTCTTCCTGTATTAGCAGGGGCTATTACTATATTATTAACTGA TCGTAATTTAAATACATCTTTTTTTGATCCTGCTGGAGGAGGAGATCCAA TTTTATATCAACATTTATTTTGATTTTTTGGT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/unnamed-scaled.jpg,",31.9708128000,-81.1586515000,08/26/2025,"
\n
Sweep Net<\/span><\/li>\n
Behind police station<\/span><\/li>\n
Tall grass<\/span><\/li>\n
Shaded area<\/span><\/li>\n<\/ul>\n",Libellulidae,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-11-141902.png,","
DNA-extraction of kit in-house reagents was used<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/tempImageXeIwNy-scaled.jpg,",,,08/26/2025,"
Collection was August 26, 2025 and the insect was wild caught with the Ariel net weep method in a group of green prickly plants outside of the Learning Commons building.<\/p>\n",Hymenoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/ZoeViolet-scaled.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,ATTGGAACATCCTTAAGATTAATTATTCGATTAGAACTAGGAACTCCaat ttCAATAATCAATAATGATCAAATTTACAACTCAATCATTACAGCACATG CATTAATTATAATTTTTTTTATAGTTATACCATTTATAATTGGGGGGTTC GGAAATTGATTAATTCCATTAATATTAGGAGCCCCAGATATAGCTTTCCC ACGAATAAATAATATAAGATTCTGACTTCTACCACCATCTCTTATATTAT TATTAATAAGAAATATTAATGATATAGGAGTAGGAACAGGATGAACTTTA TATCCACCCCTATCATCAACTATAGGACACAATTCATCATCAGTAGATTT TGCTATTTTTTCACTCCACATTGCAGGAATTTCATCAATCATAGGAGCAA TTAATTTTATTGTAACCATCTTAAATATACATATTAAAACCCATTCATTA AATTTTTTACCTATATTTACATGATCAGTTTTAATCACAGCAATCTTATT ACTATTATCTTTACCAGTTCTAGCAGGAGCTATTACTATACTATTAACTG ACCGAAACTTAAATACATCATTTTTTGACCCTTTAGGAGGAGGAGACCCA ATTTTATTTCAACATTTATTTTGATTTTTTGGT,,, "",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_5249-scaled.jpeg,",31.9708128,-81.15865149999999,08/26/2025,"
Date of collection:
\n-08\/26\/25
\nTime of collection:
\n– 1:30-2:00
\nLocation of collection:
\n– Lane Library, Georgia Southern university
\nEnvironment of collection site:
\n– Bushes and flowers in a sunny location
\nMethod of collection:
\n-Ariel\/ Sweep Net above flowers and bushes<\/p>\n",Hymenoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/ChaeliCharleigh-1.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,AGGTTCATCTATAAGATTAATTATTCGTATAGAACTAGGTATagcagttt cgttaCTTAAAAATGAACAATTATTTAATTCAATCGTTACTTCACATGCA TTCGTAATAATTTTCTTTTTTATTATGCCTCTAATAATTGGAGGTTTTGG CAATTGATTAATTCCTCTAATATTGGGAGTACCTGATATAGCTTTCCCTC GATTAAATAATTTAAGATTTTGACTATTACCTCCCTCTCTTATTTTATTA ATTTTTTCTAACTTTGTAGGAAAAGGAATTGGGACAGGATGAACTATTTA TCCCCCACTATCTAATTCTATTTACCATACTGGTATAGCAGTAGATTTAT TAATTTATTCCCTACATTTAAGAGGAATTTCTTCAATTCTAGGCTCAATT AATTTTATTGTAACAATTTTTAAAATACATAATTCTAATATTAAAATACA TTTAGTAATATTATTTCCTTGATCTATTTATATTACTACAATTTTATTAT TACTTTCATTACCAGTTTTAGCTGGGGCAATTACTATACTTTTATTTGAT CGAAACTTTAATACTTCATTTTTTGATCCCTCAGGAGGAGGAGATCCAGT TTTATATCAACATTTATTTTGATTTTTTGGTCA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_8182-scaled.jpg,",31.9791165937,-81.1613849570,08/26/2025,"
Collected in a floral garden habitat with an aerial sweep net<\/p>\n",Hymenoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/ChaeliCharleigh.jpeg,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,TTGATcTGGATAGTAGGAACTTCTTTAAGTGTTTTAATTCGTTTGGAACT TGGTActcctttccgattTATTGGAAATGATCAAATTTATAATAGAATTG TAACTGGACATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATA ATTGGAGGATTTGGTAATTGATTAGTTCCTTTAATATTAGGGTGTCCAGA TATAGCTTATCCGCGATTAAATAATATAAGTTATTGATTATTACCCCCTT CTTTAACTTTATTAATTATAAGAAATTTTATTGGTGATGGAGTTGGAACA GGTTGAACAGTTTATCCTCCTTTATCATCTATTCCATATCACTCTGGTAT ATCTGTTGATTTATCAATTATTTCTTTACATATTGCTGGAATTTCTTCTA TTTTAGGAGCTATTAATTTTATAGTAACTATTGTAAATATACATATTAAA ATTTTAAAATTTGATATTTTACCTCTATTACCTTGATCAATTTTTATTAC AGCTTCATTACTTTTATTATCTTTACCTGTTTTAGCAGGAGCTATTACAA TATTATTATTAGACCGTAATTTGAATACTTCTTTTTTTGATCCTTCTGGT GGGGGTGATCCAATTTTATATCAACATCTATTTTGATTTTTTGGTC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-11-140348.png,",31.9750144,-81.1597824,08/26/2025,"
Cool shaded area next to a wasp nest underneath a window seal<\/span><\/p>\n",Hymenoptera,,Partial abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-11-140926.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,ATCAGGAATAaTTGGaaCATCCTTAAGATTAATTATTCGATTAGAACTAG GAACTcccatttcggtAATCAATAATGATCAAATTTACAACTCAATCATT ACattacaTGCATTAATTATAATTTTTTTTATAGTTATACCATTTATAAT TGGGGGGTTCGGAAATTGATTAATTCCATTAATATTAGGAGCCCCAGATA TAGCTTTCCCACGAATAAATAATATAAGATTCTGACTTCTACCACCATCT CTTATATTATTATTAATAAGAAATATTAATGATATAGGAGTAGGAACAGG ATGAACTTTATATCCACCCCTATCatCAACTATAGGACACAATTCATCAT CAgaaGATTTTGCTATTTTTTCACTCcacA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-11-at-2.07.30 PM.png,",,,08/26/2025,"
\n
Time of Collection: 1:23 pm<\/span><\/li>\n
Location of collection: Outside of Lane Library<\/span><\/li>\n
Description of environment: Flower bushes and shrubs<\/span><\/li>\n
Method of Collection: Aerial Sweep Net<\/span><\/li>\n<\/ul>\n",Diptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-11-at-2.04.35 PM.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-11-at-1.58.20 PM.png,",,,08/26/2025,"
<\/p>\n\n\n\n\n\n\n\n
Date Collected<\/span><\/td>\n 8\/26\/25<\/span><\/td>\n<\/tr>\n
Time Collected<\/span><\/td>\n 1:35 PM<\/span><\/td>\n<\/tr>\n
Location Of Collection\u00a0<\/span><\/td>\n By Arts Drive by the mailroom<\/span><\/td>\n<\/tr>\n
Habitat<\/span><\/td>\n Garden, tall grasses, and weeds<\/span><\/td>\n<\/tr>\n
Method of collection\u00a0<\/span><\/td>\n Sweep Net<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n",Coleoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/LenahAbby-1.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,AACCTCCCTTagaCTACTAATTcgagcagaACTcggaAatccccttttgg gtgATTGGAGAcgaCCAAATTTACAATGTTATTGttccttttcATGCTTT CATCATAATTTTTTTCATGGTAATACCAATTATAATTGGTGGATTCGGAA ACTGGCTTGTCCCTCTAATACTTGGGGCCCCTGACATagcTTTCCCCCGA ATAAATAACATAAGATTTTGGCTTCTTCCTCCTTCTTTGACTTTACTTTT AATGagaAGATTagtaGAAAGAGGAGCTGGAACTGGATGAACAGTATACC CCCCTTTATCTAGAAATATCGCTCACAGAGGGGCTTCTGTAGATCTAGCT ATTTTTAGTTTACACCTAGCTGGAATTTCATCTATTCTTGGAGCTGTAAA TTTCATTACTACAGTTATTAACATACGTTCTACAGGCATAACATTTGACC GAATACCATTATTTGTATGATCAGTAGCTTTAACaactCTTTTACTACTT CTGTCCCTGCCTGTCCTAGCTGGCGCAATCACGATACTTTTAACTGATCG AAATATTAATACCTCATTCTTTGACCCAGCGGGTGGTGGAGACCCAATTT TATACCAACACTTATTTTGATTTTTTGGTCAC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_8217-scaled.jpg,",31.9708128,-81.15865149999999,08/26/2025,"
\n
Location: south of Science Center on Georgia Southern University Armstrong campus<\/span><\/li>\n
Habitat: pine straw bedding at the base of a tree<\/span><\/li>\n
Collection method: sweep net<\/span><\/li>\n<\/ul>\n",Blaberidae,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/gel-image-1.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,TGGATAGTAGGGACATCTTTAAGAATATTAATCCGAGCAGAACTAAATCA ACCTgttTCATTAATTGGAGATGATCAAATCTATAATGTTATTGTAACGG CTCACGCTTTTGTTATAATTTTCTTTATAGTTATGCCAATTTTAATTGGA GGATTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGC ATTTCCTCGTATAAATAATATAAGTTTTTGACTTTTACCTCCATCTCTAT CTCTTCTATTAGCCAGTAGCCTTGTCGAAAGAGGAGCTGGGACTGGTTGA ACCGTATACCCTCCCTTAGCTAGAGGAATTGCTCATGCTGGAGCTTCAGT AGATTTAGCTATTTTCTCATTACATCTTGCAGGTGTATCATCAATTTTAG GTGCCGTAAATTTTATTTCAACAATTATTAACATGAAACCAATTAATATA AGCCCTGAACGAATTCCTTTATTTGTTTGGTCAGTAGGTATTACTGCATT ATTATTATTATTATCCTTACCAGTTCTTGCAGGTGCAATTACAATATTAT TAACAGACCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGGGGAGGT GATCCTATTTTATATCAACATTTATTTTGATTTTTTGGTC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_3471-scaled.jpeg,",31.9708128,-81.15865149999999,08/26/2025,"
Caught while crawling through straw on the ground.<\/p>\n
Brown and small body<\/p>\n",Insecta,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-11-1.44.28-PM.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-09-12-214227.png,",31.9708128000,-81.1586515000,08/26/2025,"
The specimen was collected\u00a0 from a wild environment near Georgia Southern University, Armstrong Campus, Savannah, GA. A photograph of the specimen was taken and uploaded. The specimen was observed in its natural habitat prior to collection.<\/p>\n",Drosophila melanogaster,,savannah ga,Standard electrophoresis system,1X TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-11-114823.png,","
A DNA extraction kit using in-house reagents was used to isolate genomic DNA from the insect specimen.<\/p>\n",No,TGGAACATCTTTAAGAATTTTAATTCGAGCTGAATtattacATCCTGGAGCATTAATTGGAGATGATCAAATTTATAATGTAATTGTAACTGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGTGGATTTGGAAATTGATTAGTGCCTTTAATATTAGGTGCTCCTGATATAGCATTCCCACGAATAAATAATATAAGATTTTGACTTCTACCTCCTGCTCTTTCTTTACTATTAGTAAGTAGAATAGTTGAAAATGGAGCTGGGACAGGATGAACTGTTTATCCACCTCTATCCGCTGGAATTGCTCATGGTGGAGCTTCAGTTGATTTAGCTATTTTTTCTCTACATTTAGCAGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACAACTGTAATTAATATACGATCAACAGGAATTTCATTAGATCGTATACCTTTATTTGTTTGATCAGTAGTTATTACTGCTTTATTATTATTATTATCACTTCCAGTACTAGCAGGAGCTATTACTATATTATTAACAGATCGAAATTTAAATACATCATTTTTTGACCCAGCGGGAGGAGGAGATCCTATTTTATACCAACATTTATTTTGATTTTTTGGTCA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_5833-1-scaled.jpg,",31.9790044034,-81.1609234475,08/26/2025,"
Was found in dirt\/soil under pine straw near sidewalk using the dirt pan method.<\/p>\n",Hymenoptera,,head and lower body,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-4.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,CAGggatTATAGGATCTTCCATAAGCCTAATAATTCGCCTTGAATTAGGC TCctcctttaACTTAATTAATAATGATCAAATCTATAATTCTTTAATTAC TAATCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTG GAGGATTTGGAAATTTCTTAGTTCCCCTAATATTAGGATCACCTGATATA GCCTATCCTCGAATAAATAATATAAGATTCTGATTACTCCCTCCATCATT AATTTTATTAATTTCTAGAAATTTTATTTTTATAGGATCGGGTACAGGAT GAACAGTTTACCCCCCTTTGTCATCTAATATCTTCCATAATGGACCTTCC GTTGATTTAACTATCTTCTCTTTACACATAGCAGGTATATCATCTATTTT AGGGGCTATTAATTTTATTGCAACTATTTTAAATATACATCATAAATTAT TATCTAAAGATAAAATCCCCCTTCTTATCTGATCAATTTTAATTACTGCA ATCCTCCTTCTATTATCACTCCCCGTTCTTGCAGGAGCTATTACTATACT TTTAACAGACCGAAATCTAAATACCTCTTTCTTTGACCCCGCGGGAGGGG GGGACCCAATTTTATACCAACATTTATTTTGATTTTTTGGTCACCC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/850ebeac-6504-43b1-9e8c-869a94661293.jpg,",31.9708128,-81.15865149999999,08/26/2025,"
Collected from the bushes, trees, and grassy areas
\nMethod: sweeper and tray<\/p>\n",Hymenoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Gel-Electrophoresis-Results.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_9566-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_9569-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_9575-1-scaled.jpg,",31.980629270317852,-81.16002802092164,08/26/2025,"
\n
Location: lawn near the Science Center\u00a0<\/span><\/li>\n
Habitat: Tall and dead grass lawn\u00a0<\/span><\/li>\n
Method: Sweep Net <\/span><\/li>\n<\/ul>\n",Diptera,,Rear half,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_9975-1.jpeg,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,TGATCTGGTATAGTAGGATCATCAATAAGATGAATTATTCGAATTGAACT AGGACAACCcgttTcagttATTGGAGATGATCAAATTTATAATGTTATTG TAACtgttCATGCCTTCATTATAATTTTTTTTATAGTAATACCTATTATA ATTGGAGGATTTGGTAATTGATTAGTACCTTTAATAATTGGAGCCCCTGA CATAGCATTTCCCCGAATAAATAATATAAGATTCTGATTATTACCCCCCT CTTTAATATTATTAATTATTAGAAGAACAGTGGAAATAGGGGTAGGCACT GGATGAACAGTTTACCCCCCATTATCAAGCAATTTATCACATAGAGGAGC CTCAGTTGATTTAGCAATCTTCTCCTTACATTTAGCAGGCATTTCATCAA TCTTAGGGGCAGTAAATTTTATTTCAACAATTATTAATATACGACCAAAG GGGATAATTTTAGAACGAACCCCATTATTCGTTTGATCTGTTGGAATTAC AGCCTTATTGCTACTATTATCTTTACCTGTATTAGCAGGGGCTATCACTA TATTATTAACTGATCGAAATTTTAATACTTCTTTCTTTGACCCTACTGGT GGTGGGGATCCTATTTTATATCAACACTTATTCTGATTTTTTGGTCAC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/CamScanner-09-12-2025-10.51_1-scaled.jpg,",,,,"",Coleoptera,,savannah ga,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-11-110953.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",Unknown,AGCTGGTATAGTTGGAACTTCACTTAGACTTCTAATTCGAGCAGAATTAG GAAACCccttgtCTTTAATTGGTGATGATCAAATCTATAATGTAATTGTA AcatccCATGCTTTTATCATAATTTTTTTCATAGTAATACCAATTATAAT TGGTGGATTTGGAAATTGATTAGTACCTTTAATACTAGGAGCTCCTGATA TAGCATTTCCTCGAATAAACAATATGAGATTCTGGCTACTCCCCCCATCT CTTACTCTTCTTTTAATGAGAAGATTAGTAGAGAATGGAGCCGGAACTGG TTGAACAGTGTATCCCCCACTTTCAGCTAATATTGCCCATAGAGGAGCAT CAGTTGATTTAGCTATTTTTAGCCTTCATTTAGCTGGAATTAGGTCTATT TTAGGTGCAGTAAATTTTATTACTACAGTAATTAATATACGATCTACAGG AATAACATTTGATCGAATACCTTTATTTGTATGATCAGTAGTATTAACAG CATTACTCCTTTTACTTTCTTTGCCAGTTTTAGCTGGAGCTATTACAATA CTTCTAACAGATCGTAATATTAACACAACATTCTTTGACCCAGCAGGAGG TGGAGATCCTATTTTATATCAACACTTATTTTGATTTTTTGGTCA,,, "",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0010-1-scaled.jpeg,",31.979553938508744,-81.1616317030341,08/26/2025,"",Hymenoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot_11-11-2025_103814_docs.google.com_.jpeg,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_2593-scaled.jpeg,",31.9811743308,-81.1598704418,08/28/2025,"
Caught using drag net.<\/p>\n",Pentatomidae,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_2645-scaled.jpg,","
DNA extraction kit of in-house reagents was used<\/p>\n",Yes,CGGATATGAGCAGGTATAGTAGGATCCGCTATAAGACTAATTATCCGAAT CGAACTAGGACAACccggtagatTCATTGGGGATGATCAAATTTATAACG TAATCGTAACagctCACGCCTTCGTAATAATTTTCTTCATAGTAATACCT ATTATAATTGGAGGATTTGGAAACTGACTAGTTCCCCTAATAATCGGAGC TCCCGACATAGCATTCCCTCGTATAAATAACATAAGATTTTGATTATTAC CTCCTTCATTAACATTATTAATAATAAGTAGACTAGCAGAATCAGGAGCT GGAACCGGATGAACAGTTTACCCTCCTTTATCAAGTAATTTATCTCACAG AGGAGCATCCGTAGACTTAGCAATTTTTTCACTCCACTTAGCCGGGGTAT CTTCAATCTTGGGAGCAGTAAATTTTATTTCCACAATCATTAATATACGA CCCGCAGGAATAATCCCTGAACGAATTCCTTTATTTGTATGATCGGTAGG GATTACTGCCTTACTTCTACTACTATCATTACCTGTGCTAGCAGGTGCTA TTACAATATTATTAACTGATCGAAATTTTAACACATCATTTTTTGATCCC TCAGGTGGAGGAGACCCTATTTTATACCAACATTTATTTTGATTTTTTGG TCAC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/ant-drawing-labled-improved-jpeg-scaled.jpg,",31.980414574211242,-81.15042205970828,08/26/2025,"
In the soil under pine straw.<\/p>\n",Hymenoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/jpeg-scaled.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,TAATTGGATCTTCTTTAAGAATAATTATTCGACTTGAACTTGGGACCTGt tatTCTATTATCAACAATGATCAAATTTTTAATTCTTTAGTTACAGCTCA TGCATTTATTATAATTTTTTTTATAGTTATACCATTTATAATTGGAGGAT TTGGTAATTTTCTTGTCCCTTTAATATTAGGTTCCCCAGATATAGCATAC CCCCGTATAAATAATATAAGATTTTGACTCCTGCCTCCATCTATTTCTCT CCTTCTCTTAAGAAATTTCATTAATAATGGATCTGGGACAGGATGAACTG TTTATCCCCCCTTAGCTTCTAATATTTTTCATAATGGTCCATCAATTGAT TTAACAATTTTTTCTTTACATATTGCAGGAATATCTTCAATTTTAGGAGC AATTAATTTTATTTCTACAATTATTAATATACATCATAAAAATTCATCAA TAGATAAAATTCCCCTTTTAGTTTGATCAATTCTAATTACTGCTATTCTT CTTCTCCTTTCTCTCCCAGTTTTAGCTGGAGCAATCACTATACTTTTAAC TGATCGAAACCTAAATACTTCATTTTTTGATCCTTCTGGAGGAGGAGATC CAATTCTTTATCAACATTTATTTTGATTTTTTGGT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10380-scaled.jpg,",42.8865818349,-100.5505060718,06/21/2025,"
Collected live from city park.<\/p>\n",Chironomus decorus,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20251003_174909019_2-scaled.jpg,","
Results are fourteenth row. Top: CO1. Bottom: Wol.<\/p>\n",No,CTTGATCCGGAATAGTAGGAACATCTCTAAGAATGCTTATTCGAGCAGAATTAGGACGTCCTGGTACTTTCATTGGTGATGACCAAATTTATAATGTTGTAGTTACAGCACATGCTTTTATTATAATTTTTTTCATAGTTATGCCTATTCTAATTGGTGGATTTGGTAATTGACTTGTACCTCTTATATTAGGTGCCCCAGATATGGCTTTCCCTCGAATAAATAATATAAGTTTTTGACTTCTTCCCCCTTCTCTTACACTTTTACTTTCTAGTTCATTTGTAGAAAATGGAGCGGGAACAGGATGAACAGTTTATCCTCCCCTTTCTGCGGCTATTGCCCATAGAGGTGCTTCTGTTGATTTAGCTATTTTTTCTCTTCATTTAGCAGGTGTTTCTTCTATTTTAGGATCTGTAAATTTTATTACTACAGTTATTAATATACGAGCTAATGGAATTACATTAGACCGAATACCTTTATTTGTTTGATCTGTAGTTATTACAACAGTTCTTTTACTTTTATCATTACCTGTTTTAGCTGGGGCTATTACAATGCTTTTAACTGATCGAAATTTAAATACATCATTCTTTGACCCGGCTGGTGGGGGAGACCCAATTCTATATCAACATTTATTTTGAT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10379-scaled.jpg,",42.8088206476,-102.2032171550,06/24/2025,"
Collected live from lawn.<\/p>\n",Anystis agilis,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20251003_174909019_2-scaled.jpg,","
Results are thirteenth row. Top: CO1. Bottom: Wol.<\/p>\n",No,GAGCAGGTATATTAGGAGCTGGAATAAGACTTCTGATTCGATTAGAACTAACTCAACCAGGTTCTCTTTTAGAAAATGATCAAATTTATAATACAATTGTAACTGCTCATGCTTTTGTTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGTAATTGACTAGTTCCTATTATAATTGGAGCTCCAGACATAGCGTTCCCTCGTATAAATAACATAAGATTCTGATTACTACCACCTTCTCTTTCTCTTCTTATATTATCAGCTTTTACATCAAAGGGTAGAGGAACTGGATGAACTGTATATCCTCCCTTATCAGGCAATATTTCTCATGCTGGAGCATCTGTAGATTTAACTATTTTTTCGTTACATCTTGCTGGAATTAGATCAATTTTAGGAGCAATTAATTTTATAACTACTATCATAAATATAAAAACTCCCCATTTAAGATTTGAACAAATTCCTTTATTTGTGTGATCTGTTTTTATTACTACTATTTTACTTTTATTGTCATTACCTGTTTTAGCAGGTGCGATTACCATATTGTTAACAGATCGTAATTTTAACACTTCCTTTTTTGATCCAATTGGAGGTGGAGATCCAATTTTATATCAACATTTATTTTGAT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10378-scaled.jpg,",42.8088204812,-102.2032478870,06/24/2025,"
Collected live from lawn.<\/p>\n",Udea rubigalis,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20251003_174909019_2-scaled.jpg,","
Results are twelfth row. Top: CO1. Bottom: Wol.<\/p>\n",No,GGGCAGGATAGTAGGAACATCTTTAAGTTTATTAATTCGAGCTGAATTAGGAAATCCAGGTTCATTAATTGGTGATGATCAAATTTATAATACTATTGTAACAGCCCATGCATTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGATTAATTCCATTAATATTAGGAGCTCCAGATATAGCTTTCCCTCGAATAAATAATATAAGATTTTGATTATTACCACCTTCATTAACACTCCTTATTTTTAGAAGAATCGTTGAAAATGGAGCAGGAACAGGATGAACAGTTTACCCCCCACTTTCATCCAACATTGCACATAGAGGAACTTCAGTAGATTTAGCTATTTTTTCCCTTCATTTAGCTGGAATTTCATCAATCTTAGGAGCAATTAATTTTATTACTACAATTATTAATATACGAATTAATGGTTTATCATTTGATCAAATACCCCTATTTGTTTGAGCTGTCGGTATTACAGCTTTACTATTATTACTTTCTTTACCAGTCTTAGCAGGAGCTATTACTATACTCTTAACTGATCGTAATTTAAATACATCATTTTTTGACCCTGCCGGAGGTGGAGACCCAATTCTTTATCAACATTTATTTTGATT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10376-scaled.jpg,",42.808403319730736,-102.20761184110032,06/23/2025,"
Collected live from a tree in the city park.<\/p>\n",Formica glacialis,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20251003_174909019_2-scaled.jpg,","
Results are tenth row. Top: CO1. Bottom: Wol.<\/p>\n",No,TGAGCAGGATAATTGGATCTTCTATAAGTATAATTATTCGTTTAGAATTAGGTTCATCTAATTCATTAATCAATAATGATCAAATTTATAATTCTTTAGTAACTAATCACGCTTTTATTATAATTTTTTTCATAGTAATACCATTTATAATTGGTGGATTTGGAAATTTTCTAATTCCTTTAATACTAGGATCACCAGACATAGCATACCCTCGTATAAATAATATAAGATTTTGACTCTTACCTCCTTCAATTACTCTTTTACTTTTAAGAAATTTTATTAATGATGGAACAGGTACAGGATGAACTATTTATCCTCCTTTATCCTCTAATATTTTTCATAATGGTCCTTCTGTAGATTTAACAATTTTTTCTCTTCATATTGCAGGTATATCCTCAATTTTAGGAGCAATTAATTTTATTTCAACAATTCTTAATATACATCATAAAAATTTTTCTATTGATAAAATTCCTTTACTCGTATGATCAATTTTAATTACAGCTATCTTACTCCTATTATCATTACCTGTCTTAGCCGGAGCTATTACTATACTATTAACTGACCGAAATTTAAATACTTCATTTTTTGATCCTTCAGGAGGAGGTGACCCAATTCTATACCAACATTTATT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10373-scaled.jpg,",42.8083944943,-102.2076434856,06/23/2025,"
Caught live in city park.<\/p>\n",Toxomerus marginatus,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20251003_174909019_2-scaled.jpg,","
Results are ninth row. Top: CO1. Bottom: Wol.<\/p>\n",No,GAGCTGGATTAGTTGGTACATCATTAAGTATTTTAATTCGTGCAGAACTTGGACATCCAGGTGCATTAATTGGAGATGATCAAATTTATAATGTAATCGTAACTGCTCATGCATTTGTAATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCTCCTGATATAGCATTTCCTCGAATAAATAATATAAGATTTTGATTATTACCTCCTTCATTAACATTATTATTAGTAAGTAGTATGGTTGAAAATGGAGCCGGAACAGGTTGAACAGTTTATCCTCCTCTTTCAGCTAATATTGCACATGGTGGAGCTTCAGTTGATTTAGCAATTTTTTCACTTCATTTAGCTGGTATATCTTCAATTTTAGGAGCAGTAAATTTTATTACAACAGTAATTAATATACGATCAAATGGTATTTCATACGATCGAATACCATTATTTGTATGATCAGTAGTAATTACAGCTCTTTTACTTTTATTATCATTACCAGTTTTAGCAGGAGCTATTACTATATTATTAACTGATCGAAATTTAAATACTTCATTTTTCGATCCTGCTGGAGGTGGTGATCCAATTTTATACCAACATTTATTTTGATT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10372-scaled.jpg,",42.8083991472,-102.2076311841,06/23/2025,"
Collected live from city park.<\/p>\n",Culex restuans,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20251003_174909019_2-scaled.jpg,","
Results are eighth row. Top: CO1. Bottom: Wol.<\/p>\n",No,AAGTATTCTTATTCGAGCAGAATTAAGTCAACCTGGAGTATTTATTGGAAATGATCAAATTTATAATGTTATTGTAACTGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGTAATTGATTAGTTCCTCTAATATTAGGAGCTCCTGATATAGCTTTTCCTCGAATAAATAATATAAGTTTTTGAATACTACCTCCTTCATTAACATTACTACTTTCAAGTAGTATAGTAGAAAATGGAGCTGGGACTGGATGAACAGTTTACCCCCCTCTTTCATCTGGTACAGCCCATGCTGGAGCTTCAGTAGATTTAGCTATTTTTTCATTACATTTAGCTGGAATTTCATCAATTTTAGGAGCAGTAAATTTTATTACTACTGTAATTAATATACGATCTTCAGGTATTACACTTGATCGAATACCATTATTTGTTTGATCAGTAGTAATTACTGCTGTTCTTTTACTTCTTTCTTTACCTGTATTAGCTGGTGCTATTACTATACTATTAACTGATCGAAATCTAAATACTTCGTTCTTTGATCCTATTGGAGGAGGAGATCCAATTTTATATCAACATTTATTTTGATT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10371-scaled.jpg,",42.8084066851,-102.2076193696,06/23/2025,"
Collected live in city park.<\/p>\n",Myospila meditabunda,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20251003_174909019_2-scaled.jpg,","
Results are seventh row. Top: CO1. Bottom: Wol.<\/p>\n",No,TTGATCAGGTATAATTGGTACTTCATTAAGAATTTTAATTCGGGCTGAATTAGGACATCCTGGTGCTTTAATTGGAGATGATCAAATCTATAATGTAATTGTAACAGCACATGCTTTTATTATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCTCCAGATATAGCATTTCCACGAATAAACAATATAAGTTTTTGATTACTACCTCCTGCTTTAACATTATTGTTAGTTAGTAGTATAGTTGAAAATGGAGCTGGAACTGGATGAACTGTTTACCCTCCGCTTTCTTCTAATATTGCTCATGAAGGAGCTTCTGTTGATTTAGCAATTTTTTCTCTACATTTAGCTGGAATTTCCTCAATTTTAGGAGCTGTAAATTTTATTACTACTGTAATTAATATACGATCAACTGGAATTACATTTGACCGAATACCTTTATTTGTATGATCAGTAGTAATTACAGCTTTATTATTATTATTATCTCTACCTGTATTAGCAGGAGCTATTACAATACTATTAACAGATCGAAATATAAATACTTCTTTTTTTGATCCTGCTGGCGGAGGAGACCCAATTCTATATCAACATTTATTTTGATT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10370-scaled.jpg,",42.8084059050,-102.2076091589,06/23/2025,"
Collected live crawling in grass in city park.<\/p>\n",Chlamydatus wilkinsoni,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20251003_174909019_2-scaled.jpg,","
Results are sixth row. Top: CO1. Bottom: Wol.<\/p>\n",No,GAATTGAACTAGGTATACCTGGCTCATTTATCGTAGACGATCAAACTTATAATGTAGTAGTAACAGCCCACGCATTCATTATAATTTTCTTCATAGTTATACCAATTATAATTGGTGGGTTTGGAAACTGGCTAATACCNTCAATAATTGGAGCCCCCGATATGGCATTCCCTCCAATAAATAATATAAGATTTTAATTACTCCCCCCCCNTATTACATTATTATTAACTAGAAGAATANTAAAAAATGGAGCAGGAACTGGATGAACATTCTTCCCCCCCTCCCCTNTTNNTTTTGACNTGCGGGGGNCTCGATNTAATTAACATCTTTTTATTTCNCTCTTCNNNGTGTCATANNACCTTNTATCCGAGAATTTTATCTTCTTANTTCTANNATAATAATCAAANGAATTACCGTTTNACCTATTCCNCNNATNCNATTTCTTGGATGAATCTCAACNTCTACTTGATTGCTTTCCCTTTCNTTACCTNCGGNNCNTGNCCGAACCCCTCACAGATTGTAAATATANNNATCNTTCTTCTCTTCCTNTTGCGGNGGNGGANGGNGNNGANANCANACTTATATNACATTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10369-scaled.jpg,",42.8677683083,-100.5494266024,06/21/2025,"
Collected live near a light fixture.<\/p>\n",Isonychia rufa,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20251003_174909019_2-scaled.jpg,","
Results are fifth row. Top: CO1. Bottom: Wol.<\/p>\n",No,GGGCAGGAATAGTAGGAACTTCCCTTAGTCTTTTAATTCGAGCTGAGTTGGGACAACCAGGATCATTAATTGGTGACGATCAAATTTATAATGTTATTGTTACAGCTCATGCTTTCGTTATAATTTTCTTCATGGTTATACCCATTATAATTGGAGGGTTTGGAAATTGATTGGTACCTCTTATACTTGGAGCTCCCGATATGGCCTTCCCACGAATAAATAATATAAGATTTTGACTTTTACCTCCAGCCTTAACTTTACTCTTGGCCAGCAGTATAGTAGAAAGAGGAGCTGGCACCGGGTGGACTGTTTACCCCCCACTTTCTGCGGGTATTGCCCATGCAGGAGCTTCAGTAGATCTTGCTATCTTTTCTCTTCATTTAGCAGGAGTTTCTTCTATTTTAGGAGCAGTAAATTTTATTACTACTACTATTAACATACGTTCTAGTGGAATAACTATAGATCGTATTCCCCTCTTTGTTTGATCTGTACTTATTACTGCTATTCTCCTCCTTTTATCCCTCCCAGTCCTTGCCGGAGCAATTACCATATTGTTAACAGACCGAAACTTAAATACTTCCTTTTTTGACCCAGCAGGAGGGGGAGATCCTATTTTATATCAACATCTCTTTTGATTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10368-scaled.jpg,",42.8677817419,-100.5494372031,06/21/2025,"
Collected live from a lampshade.<\/p>\n",Perlesta decipiens,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20251003_174909019_2-scaled.jpg,","
Results are fourth row. Top: CO1. Bottom: Wol.<\/p>\n",No,GGGGACCTCATTAAGTCTTCTCATTCGAGCAGAACTCGGTCAACCCGGATCTCTCATTGGAGACGACCAAATTTACAATGTTATTGTTACCGCTCACGCTTTCGTTATAATTTTCTTCATAGTTATACCCATTATAATTGGTGGGTTTGGGAATTGACTTGTTCCATTAATGCTCGGTGCGCCTGACATGGCCTTCCCACGAATAAATAACATAAGTTTTTGGTTATTACCACCCTCACTAACATTATTGTTGGCAAGTAGCCTAGTCGAAAACGGGGCGGGAACAGGTTGAACAGTTTACCCACCGCTTTCTGCCGGAATCGCTCATGCTGGAGCGTCTGTTGACATGGCCATTTTCTCCCTCCATTTAGCAGGTGTATCCTCTATTCTTGGGGCTGTAAATTTCATTACTACAGTAATTAATATACGATCGGCAGGTATATCACTAGACCGAATACCTTTATTCGTATGAGCTGTTGCTATTACAGCTCTTCTCCTTTTATTATCCCTGCCAGTTCTTGCCGGAGCCATCACTATACTCCTAACAGACCGTAACTTAAATACATCTTTCTTTGACCCCGCAGGTGGGGGTGACCCCATTCTATACCAACACCTGTTTTGATTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_8066-2-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_8113-1-scaled.jpg,",,,09/11/2025,"",Insecta,,Abdomen,Standard electrophoresis system,TAE,Other,"","
DNA Extraction kit of in house reagents was used<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_7833-1-1.png,",36.1569645949,-86.7983996180,09/08/2025,"
Long legs, has wings, red and black colors<\/p>\n",Coleoptera,,Abdomen,Standard electrophoresis system,1X TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_8103.jpg,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,AGGTTTAGTAGGAACTTCTTTAAGATATTAATTCGAACAGAGCTAGGTAG CcccgttatggtaATTGGAAATGACCAAATCTATAATGTAATTGTTacct ttcATGCTTTTATTATAATTTTTTTTATAGTAATACCAATTATAATTGGA GGATTTGGAAATTGATTAGTACCACTAATAATTAGGGCCCCTGATATAGC ATTCCCCCGAATAAATAATATAAGATTTTGATTATTACCCCCTTCACTAT TTTTACTAATTATAAGAAGAATTGTTGAAAATGGAGCAGGAACAGGATGA ACTGTATACCCCCCTTTATCATCTAACTTAGCTCATGAAGGATCTTCAAT TGATTTAGCAATTTTTAGACTACATTTAGCAGGAATTTCTTCTATTCTTG GAGCTATTAATTTTATTACAACAGTAATTAATATACGACCAATTGGTATA ACCCTAGATCAAATACCATTATTTGTATGGGCTGTAACAATTACAGCTAT TTTATTACTATTATCTTTACCAGTATTAGCAGGAGCTATCACAATATTAT TAACTGACCGAAATTTAAATACTTCTTTTTTTGACCCTGCAGGAGGAGGT GATCCTATCCTTTATCAACACCTATTTTGATTTTTTGGTCACCCTGGAAG T,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10367-scaled.jpg,",42.8677613046,-100.5494519796,06/22/2025,"
Collected live in hotel parking lot.<\/p>\n",Formica argentea,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20251003_174909019_2-scaled.jpg,","
Results are third row. Top: CO1. Bottom: Wol.<\/p>\n",Yes,GATAATTGGATCTTCTATAAGTATAATTATTCGTTTAGAATTAGGCTCATCAAATTCACTAATCAATAACGATCAAATTTATAATTCTTTAGTAACTAATCATGCTTTTATCATAATTTTCTTCATAGTAATACCATTTATAATTGGAGGATTTGGAAATTTTCTAATTCCTTTAATACTAGGATCTCCAGACATAGCTTATCCTCGTATAAATAATATAAGATTTTGACTCTTACCTCCCTCAATTACTCTTTTACTTTTAAGAAATTTTATTAATGATGGCACAGGTACAGGATGAACTATTTATCCCCCTTTAGCTTCAAATATTTTTCATAATGGTCCTTCTGTAGACTTAACAATTTTTTCTCTACATATTGCAGGTATATCATCAATTTTAGGAGCAATTAATTTTATTTCAACAATTCTTAATATACATCATAAAAATTTTTCTATTGATAAAATTCCTTTACTTGTATGATCAATTTTAATCACAGCTATCTTACTTCTATTATCCTTACCTGTTTTAGCCGGAGCTATTACTATACTATTAACTGACCGAAATTTAAATACCTCATTTTTTGACCCTTCAGGAGGGGGCGACCCAATTCTCTATCAACATTTATTTTGAT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10366-scaled.jpg,",42.86775150631142,-100.54941998876077,06/22/2025,"
Collected live near motel parking lot.<\/p>\n",Lasius neoniger,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20251003_174909019_2-scaled.jpg,","
Results are second row. Top: CO1. Bottom: Wol.<\/p>\n",No,GCTGGTATAATCGGTTCATCTATAAGAATAATTATTCGTCTAGAATTAGGATCATCTAATTCATTGATTAACAATGATCAAATTTATAATTCTATAGTTACAAGACATGCATTCATTATAATTTTCTTTATAGTTATGCCTTTCATAATTGGAGGATTTGGAAATTTCCTTGTACCTTTAATATTAGGCTCACCTGATATAGCTTATCCACGTATAAACAATATAAGATTTTGACTTTTACCCCCCTCAATTTCTCTTCTTATTTTAAGAAATTTTATTAATGATGGTGTTGGGACAGGATGAACTGTTTATCCTCCTTTAGCTTCAAATATTTTCCATAATGGACCTTCAGTTGATTTAACTATTTTTTCCCTCCATATTGCTGGTATATCTTCAATTTTAGGAGCTATTAATTTTATTTCAACTATCTTAAATATACATCACAAAAATTTTTCTATCGATAAAATCCCTTTACTTGTATGATCAATTTTAATTACTGCAATTTTACTTCTCTTATCCTTACCCGTTCTTGCAGGAGCTATTACTATACTTTTAACTGACCGTAATCTTAACACTTCATTTTTTGATCCATCAGGTGGCGGAGATCCTATTTTATATCAACATCTTTTTTGATT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/1000003347-scaled.jpg,",31.9781564,-81.1632035,08/25/2025,"
Insects collected near Georgia Southern University Armstrong campus, nearby Science Center parking lot. Insect collected via beating net from shrubs. Collected at approximately 6-7 pm EST.<\/p>\n",Insecta,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/gel.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,TtgGaACTTCACTAAGTATATTAATTCGAACAGAATTAAGCCATCCAGG ATTTTTTATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCCCATG CTTTTATTATAATTTTTTTTATAGTTATACCAATTATAATTGGTGGATTT GGAAACTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTTCC TCGAATAAATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTC TTCTAATAAGAAGCATAGTTGAAAACGGGGCTGGTACTGGATGAACAGTA TATCCCCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTT AGCAATTTTTTCTCTTCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTG TTAATTTTATTACTACAGTTATTAATATGCGATCTACTGGAATTACATTT GATCGTATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACT TCTCCTATCCCTCCCAGTTTTAGCCGGGGCTATTACTATACTATTAACAG ATCGAAATTTAAATACAAGTTTCTTTGACCCAGCAGGAGGGGGAGACCCT ATTTTATACCAACATTTATTTTGATTTTTTGGT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_3531.jpeg,",,,08/25/2025,"",Insecta,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Image-11-10-25-at-6.45 PM.jpeg,","
A DNA extraction kit of in-house reagents was used.<\/p>\n",No,TCTCTTTGGtttaTGAGCTGGGaCTGTAGGCACTTCTTTAAGTTTTTTAA TTCGAGTTGAGTTAGGAAGTCCTGGGCAAGTGTTAGGTAATGATCAGATT TATAATTCTGTTGTTACGGCTCATGCTTTTATTATAATTTTTTTTTTTGT TATACCATTTATAATTGGGGGGTTTGGTAATTGATTAGTTCCTTTAATAA TATCTATTCCTGACATaGCTTTTCCTCGTATAAATAATATGAGTTTTTGA TTATTACCACCTTCATTATTATTTTTAACTATAGGAATATTTATTGGGGA TGGTGCAGGGACTGGTTGAACTGTATATCCACCTCTTTCTAATTCAATTA ATCAATCAGGACCTagaGTagaTTTATCAATTTTTTCTTTACATATTGCT GGGGCATCATCTATTATAGGGGCAATAAATTTTATTATTACAATCTTTTT GTCAGTAcgaACTTCTATAGATAAAATTTTTTTATTTTGTTGATCTGTAG TAATTACAGCAATTTTATTATTATTATCTTTACCTGTTTTAGCGGGAGCA ATTACTATGTTATTATTTGATCGAAATTTTAATACTACTTTTTTCCTTCC TTTAGGGGGTgGaGATCCTGTTTTATATCAACATTTATTTTGATTTTTTG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_3829-scaled.jpg,",31.9708128,-81.15865149999999,08/25/2025,"
Red and blue beetle found in tall grass<\/p>\n",Coleoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-10-153512.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,GgTtTAGTAGGAACTTCTTTAAGAATATTAATTCGAACAGAGCTAGGTAG CCCCgtaTCATTAATTGGAAATGACCAAATCTATAATGTAATTGTTACAG CCCATGCTTTTATTATAATTTTTTTTATAGTAATACCAATTATAATTGGA GGATTTGGAAATTGATTAGTACCACTAATAATTAGGGCCCCTGATATAGC ATTCCCCCGAATAAATAATATAAGATTTTGATTATTACCCCCTTCACTAT TTTTACTAATTATAAGAAGAATTGTTGAAAATGGAGCAGGAACAGGATGA ACTGTATATCCCCCTTTATCATCTAACTTAGCTCATGAAGGATCTTCAAT TGATTTAGCAATTTTTAGACTACATTTAGCAGGAATTTCTTCTATTCTTG GAGCTATTAATTTTATTACAACAGTAATTAATATACGACCAATTGGTATA ACCCTAGATCAAATACCATTATTTGTATGGGCTGTAACAATTACAGCTAT TTTATTACTATTATCTTTACCAGTATTAGCAGGAGCTATCACAATATTAT TAACTGATCGAAATTTAAATACTTCTTTTTTTGACCCTGCAGGAGGAGGT GATCCTATCCTTTATCAACACCTATTTTGATTTTTTGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_8881-scaled.jpeg,",36.1414467823,-86.8151016938,08/25/2025,"
Caught in hallway using jar and paper.<\/p>\n",,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0798.jpeg,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,GTATGGTAGGAACATCCCTAAGTATATTAATTCGTGCTGAACTCGGGCAG CCagtctCACTAATTGGAGATGATCAAATCTATAATGTAATTGTTACTGC CCATGCTTTCATTATAATTTTCTTTATAGTAATACCAATTATAATTGGGG GATTTGGTAATTGATTAGTACCATTAATATTAGGAGCCCCAGATATAGCC TTCCCACGAATAAATAATATAAGATTCTGATTATTACCACCTTCATTAAC TTTATTACTAGCTAGCAGCATAGTAGAAAGAGGTGCCGGAACAGGATGAA CAGTATACCCACCACTAGCAAGAGGTATTGCTCATGCCGGAGCATCTGTT GATCTAGCAATTTTTTCATTACATCTAGCAGGTGTATCCTCAATTCTAGG AGCTGTTAATTTTATCTCCACAACAATTAATATAAAACCTATTAACATAA AACCAGAACGAATTCCTCTTTTTGTATGATCAGTAGCTATTACAGCATTA TTATTATTATTATCTCTACCAGTACTTGCTGGGGCAATTACTATATTATT AACTGATCGAAATCTAAATACATCCTTTTTTGATCCAGCAGGAGGAGGTG ATCCAATTTTATATCAACACTTATTCTGATTTTTTGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_4046-1.jpg,",,,08/25/2025,"
\n
Under pine straw<\/span><\/li>\n
Collected with Sweep net<\/span><\/li>\n<\/ul>\n",Insecta,,Partial abdomen,Standard electrophoresis system,1X TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/download.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,CTTGATCGGGgatgGTAGGAACTAATTTGAGAATTTTAATTCGAACAGAG TTAGGTCAGCCTGGATCTTTAATTGGAGATGATCAGATTTATAATGTTAT TGTTACTGCTCACGCTTTTATTATAATTTTTTTTATGGTAATGCCAATTA TAATTGGAGGGTTTGGTAATTGATTAGTTCCTTTAATATTGAGAGCTCCA GATATAGCTTTCCCACGAATGAATAATATAAGATTTTGGTTATTACCTCC ATCTCTGATTTTATTATTATCAGGGGGCCTAGTAGATAGAGGGGCTGGGA CAGGTTGGACAGTGTATCCTCCTTTATCATCAGTGATTTCTCATGGAGGG GCTTCAGTGGATATAACTATTTTTTCTTTACATTTGGCAGGGGTTTCTTC TATTTTGGGAGCCATTAATTTTATTACTACTGTGATTAATATACGTCCAG AAGGTTTAAAGCTTGATCGTGTTCCTTTATTTGTTTGATCAGTAGCTATT ACTGCTTTATTATTATTGCTTTCTTTACCGGTTTTAGCAGGAGCTATTAC GATATTATTAACTGATCGAAATTTAAATACATCTTTTTTTGATCCAGCCG GAGGCGGAGATCCTATTTTATATCAACATTTATTTTGATTTTTTGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-10-151419.png,",31.9708128,-81.15865149999999,08/12/2025,"
The insect has a three-segmented body: a head, an abdomen, and a thorax. It has mouth parts, a mandible, and entanas and is small in size.<\/p>\n",Hymenoptera,tray,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-10-30-145948.png,","
An in-house DNA extraction kit was used.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10365-scaled.jpg,",42.886573258487665,-100.55053497214483,06/21/2025,"
Collected live from city park.<\/p>\n",Lasius neoniger,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250715_211008663-scaled.jpg,","
Results are thirteenth row. Top: CO1. Bottom: Wol.<\/p>\n",No,GAGCTGGTATAATCGGTTCATCTATAAGAATAATTATTCGTCTAGAATTAGGATCATCTAATTCATTGATTAACAATGATCAAATTTATAATTCTATAGTTACAAGACATGCATTCATTATAATTTTCTTTATAGTTATGCCTTTCATAATTGGAGGATTTGGAAATTTCCTTGTACCTTTAATATTAGGCTCACCTGATATAGCTTATCCACGTATAAACAATATAAGATTTTGACTTTTACCCCCCTCAATTTCTCTTCTTATTTTAAGAAATTTTATTAATGATGGTGTTGGGACAGGATGAACTGTTTATCCTCCTTTAGCTTCAAATATTTTCCATAATGGACCTTCAGTTGATTTAACTATTTTTTCCCTCCATATTGCTGGTATATCTTCAATTTTAGGAGCTATTAATTTTATTTCAACTATCTTAAATATACATCACAAAAATTTTTCTATCGATAAAATCCCTTTACTTGTATGATCAATTTTAATTACTGCAATTTTACTTCTCTTATCCTTACCCGTTCTTGCAGGAGCTATTACTATACTTTTAACTGACCGTAATCTTAACACTTCATTTTTTGATCCATCAGGTGGCGGAGATCCTATTTTATATCAACATCTTTTTTGATT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10363-scaled.jpg,",42.8865672870,-100.5505230427,06/21/2025,"
Collected live from city park.<\/p>\n",Camponotus pennsylvanicus,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250715_211008663-scaled.jpg,","
Results are twelfth row. Top: CO1. Bottom: Wol.<\/p>\n",Yes,GGGATAATTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTAGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTAATTCCACTTATACTAGGATCTCCTGATATAGCTTACCCTCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGAACTATCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTAGTATGATCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGCGCTATTACAATACTACTAACAGACCGAAATCTTAATACTTCATTTTTCGATCCCTCGGGAGGAGGAGATCCTATTTTATACCAACATTTATTTTGATT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10362-scaled.jpg,",42.886565250998345,-100.55055920422974,06/21/2025,"
Collected live in city park.<\/p>\n",Aedes vexans,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250715_211008663-scaled.jpg,","
Results are eleventh row. Top: CO1. Bottom: Wol.<\/p>\n",No,GGGAACATCTTTAAGTATATTAATTCGTGCTGAATTAAGTCACCCAGGGATATTTATTGGAAATGATCAAATTTATAACGTAATTGTTACAGCTCATGCATTTATTATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCTCCTGATATAGCATTTCCTCGAATAAATAATATAAGTTTTTGAATATTACCTCCTTCTTTAACTCTACTACTTTCTAGTTCAATAGTAGAAAATGGAGCAGGGACAGGATGAACAGTTTATCCTCCTCTTTCATCAGGAACAGCTCACGCTGGAGCTTCTGTTGATTTAGCAATTTTCTCTCTTCATTTAGCAGGAATTTCATCTATTTTAGGAGCAGTAAATTTTATTACTACTGTTATTAATATACGATCATCTGGAATTACTTTAGATCGATTACCTTTATTTGTTTGATCTGTAGTAATTACTGCTATTTTATTACTTTTATCTCTTCCTGTATTAGCTGGAGCTATTACTATATTATTAACGGATCGAAATTTAAATACTTCCTTCTTTGACCCAATTGGGGGAGGAGACCCAATTCTTTATCAACATTTATTTTGATT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10361-scaled.jpg,",42.8865753156,-100.5505430795,06/21/2025,"
Collected live in city park.<\/p>\n",Nabis americoferus,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250715_211008663-scaled.jpg,","
Results are tenth row. Top: CO1. Bottom: Wol.<\/p>\n",Unknown,GGTATATTAGGAACATCACTTAGTTGACTTATTCGTATTGAATTAGGACACCCTGGGTCCTTTATTGGAGATGATCAAATTTATAATGTAGTTGTTACTGCACATGCTTTCATTATAATTTTCTTCATAGTAATACCAATCATAATTGGGGGATTTGGTAACTGACTAGTACCATTAATAATTGGAGCCCCCGATATGGCATTTCCCCGTATAAATAATATAAGTTTTTGACTATTACCACCCTCAATTACTCTATTAATTATTAGTAGAATTGTAGAAAAGGGAGTAGGGACAGGATGAACAGTTTATCCCCCATTATCAGCAAACGTAGCCCATAACGGTGCATCCGTAGATCTAGCCATTTTCAGATTACATTTAGCAGGTGTTTCTTCTATCCTAGGAGCAGTAAATTTTATTTCAACAATCATTAATATACGACCTGTAGGTATAACGCCAGAACGAATCCCCTTATTTGTATGATCTGTAGGTATTACAGCCCTACTACTTTTACTATCACTACCTGTATTAGCAGGAGCAATTACCATACTACTTACTGATCGTAACTTTAATACATCATTCTTTGACCCTGCAGGGGGAGGAGATCCTATTCTATATCAACATCTATTCTGATTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10360-scaled.jpg,",44.37051586370033,-103.75298744726561,06/21/2025,"
Collected live near campground.<\/p>\n",Calliphora vicina,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250715_211008663-scaled.jpg,","
Results are ninth row. Top: CO1. Bottom: Wol.<\/p>\n",No,GGATAATTGGAACTTCATTAAGAATTCTAATTCGAGCCGAACTAGGACATCCTGGAGCATTAATTGGAGATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGATTTGGTAATTGATTAGTCCCTTTAATATTAGGAGCTCCAGATATAGCCTTCCCTCGGATAAACAATATAAGTTTCTGACTTTTACCTCCTGCATTAACTTTACTATTAGTAAGTAGTATAGTAGAAAACGGAGCTGGAACAGGATGAACTGTTTACCCACCTTTATCTTCTAATATCGCTCATGGAGGAGCTTCTGTTGATTTAGCTATTTTTTCTTTACACTTAGCAGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACTACAGTTATTAATATACGATCAACAGGAATTACATTCGACCGAATACCATTATTTGTTTGATCTGTAGTAATTACAGCTTTATTACTTTTATTATCTTTACCAGTATTAGCAGGTGCTATTACTATATTATTAACAGATCGAAATCTTAATACATCATTCTTTGACCCAGCAGGAGGAGGAGACCCAATCTTGTACCAACATTTATTTTGATTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10359-scaled.jpg,",44.3704928557,-103.7530518203,06/21/2025,"
Collected live near campground.<\/p>\n",Sitobion avenae,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250715_211008663-scaled.jpg,","
Results are eighth row. Top: CO1. Bottom: Wol.<\/p>\n",No,TAATTCTATTATTAATAATAATCAATTATATAATGTAATTGTTACAATCCATGCTTTTATTATAATTTTTTTTATAACTATACCAATTGTTATTGGTGGTTTTGGAAATTGATTAATTCCTATAATAATAGGATGTCCTGATATATCATTTCCACGTTTAAATAATATTAGATTTTGACTACTACCACCATCATTAATAATAATAATCTGTAGTTTCTTAATTAATAATGGAACAGGTACAGGATGAACTATTTACCCACCTTTATCAAATAATATTGCACATAATAATATTTCAGTTGATTTAACTATTTTTTCCTTACATTTAGCAGGAATCTCATCAATTTTAGGAGCAATTAATTTTATTTGTACAATTCTAAACATAATACCAAATAATATAAAATTAAATCAAATTCCTCTTTTTCCTTGATCAATTTTAATTACAGCTATTTTATTAATTTTATCTTTACCAGTTTTAGCTGGNGCTATTACAATATTATTAACTGATCGAAATCTAAATACATCATTTTTTGATCCAGCAGGAGGAGGANANCCNATTTTATATCAACATTTATT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10358-scaled.jpg,",44.3704851864,-103.7531161933,06/21/2025,"
Collected live near campground.<\/p>\n",Nabis alternatus,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250715_211008663-scaled.jpg,","
Results are seventh row. Top: CO1. Bottom: Wol. Wolbachia bar is somewhat faint on the picture.<\/p>\n",Unknown,TAGGAACATCACTTAGTTGACTTATTCGTATTGAATTAGGACACCCTGGATCCTTTATTGGAGATGATCAAATTTATAATGTAGTTGTTACTGCACATGCTTTCATTATAATTTTCTTCATAGTAATACCAATCATAATTGGGGGATTTGGTAACTGACTAGTACCATTAATAATTGGAGCCCCCGATATGGCATTTCCCCGTATAAATAATATAAGTTTTTGACTATTACCACCCTCAATTACTCTATTAATTATTAGTAGAATTGTAGAAAAGGGAGTAGGGACAGGATGAACAGTTTATCCCCCATTATCAGCAAATGTAGCCCATAACGGTGCATCTGTAGATCTAGCCATTTTCAGATTACATTTAGCAGGTGTTTCTTCCATCCTAGGAGCAGTAAATTTTATTTCAACAATCATTAATATACGACCCGTAGGTATAACGCCAGAACGAATCCCCTTATTTGTATGATCTGTAGGTATTACAGCCCTACTACTTTTACTATCACTACCTGTATTAGCAGGAGCAATTACTATACTACTTACTGATCGTAATTTTAATACATCATTCTTTGACCCTGCAGGGGGAGGAGATCCTATTCTATATCAACATCTATTCTGATTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10356-scaled.jpg,",44.370523533021874,-103.75303036260985,06/20/2025,"
Collected dead from a bathroom.<\/p>\n",Sciaridae,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250715_211008663-scaled.jpg,","
Results are fifth row. Top: CO1. Bottom: Wol.<\/p>\n",No,AGTAGGCACTTCTTTAAGTATTTTAATTCGTACAGAATTAGGTTATCCTAATGCTTTAATTGAAAATGACCAAATTTATAATGTAATTGTAACAGCCCATGCTTTTGTAATAATTTTTTTTATAGTTATACCTATTATAGTTGGAGGATTTGGAAATTGATTAATTCCATTAATACTATCAGCCCCAGATATAGCTTTTCCACGTTTAAATAATATAAGATTTTGATTATTACCACCATCTATATTATTATTATTAATAAGAAGTTTAGTAGAAAGAGGAACCGGAACTGGTTGAACAGTTTATCCCCCTCTTTCTTCCTCAATTGCTCATTCCGGGGCTTCAGTTGATCTATCTATTTTTTCCCTTCATTTAGCAGGAATTTCATCAATTATAGGAGCTATTAATTTTATTTCTACAATTATCAATATACGAACTCCTGGGATATCTTTTGATAAAATTCCATTATTTGTATGATCAATTATAATTACAGCTATTTTATTATTACTTTCTTTACCTGTATTAGCAGGAGCAATTACAATACTATTAACAGATCGAAATTTAAATACATCTTTTTTTGACCCTGCTAGAGGAGGAGACCCTATTTTATATCAACATTTATTTTGATTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10355-scaled.jpg,",44.3705005251,-103.7530732780,06/20/2025,"
Collected dead in a bathroom.<\/p>\n",Saucrobotys fumoferalis,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250715_211008663-scaled.jpg,","
Results are fourth row. Top: CO1. Bottom: Wol.<\/p>\n",No,GGTACTTCACTTAGTTTGTTAATTCGGGCAGAATTAGGAAACCCAGGATCTTTAATTGGAGATGATCAAATTTATAATACTATTGTAACAGCACACGCTTTCATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGTAATTGATTAGTACCTCTTATATTAGGAGCTCCTGATATAGCTTTCCCCCGAATAAATAATATAAGATTTTGACTTTTACCCCCATCATTAACTTTATTAATTTCAAGAAGAATTGTTGAAAATGGAGCAGGAACTGGATGAACTGTATACCCACCCCTTTCATCTAATATTGCTCATGGAGGTAGATCAGTTGATTTAGCAATTTTTTCTTTACATCTAGCTGGAATTTCTTCTATTTTAGGGGCAATTAATTTTATTACTACTATTATTAATATACGAATTAATGGACTTTCATTTGATCAAATACCTTTATTTGTTTGAGCTGTAGGAATTACTGCTCTTTTACTTCTTTTATCACTTCCTGTATTAGCTGGGGCAATTACTATACTTTTAACAGATCGAAATTTAAATACATCATTTTTTGATCCTGCTGGTGGAGGAGATCCCATTCTATATCAACATTTATTTTGATTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10354-scaled.jpg,",44.3704928557,-103.7530518203,06/20/2025,"
Caught live crawling on gravel near campgrounds.<\/p>\n",Dropephylla linearis,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250715_211008663-scaled.jpg,","
Results are third row. Top: CO1. Bottom: Wol. Bottom Wolbachia test is to faint to see in the picture but there is a line there.<\/p>\n",Yes,AGGGACATCCCTCAGTCTTTTAATTCGAGCTGAACTAGGGAATCCTGGCACTTTAATTGGAGATGACCAAATTTATAATGTTATTGTAACTGCCCATGCATTTATTATAATTTTTTTTATAGTAATACCCATTGTTATTGGAGGATTCGGTAACTGATTAGTTCCTCTAATACTCGGAGCACCAGATATAGCATTCCCACGAATAAACAATATAAGATTTTGACTACTACCACCTTCATTAACCCTTCTTTTAATAAGAAGAATAGTTGAAAGAGGAGCTGGAACAGGATGAACTGTGTACCCCCCACTTTCATCAAATATTGCCCACGGAGGAGCCTCAGTTGACTTAGCTATTTTCAGGCTTCATTTAGCAGGAATTTCATCAATTTTAGGGGCTGTAAATTTCATCACTACAGTTATTAATATACGATCCTCAGGAATATCATTTGATCGAATACCTTTATTTGTATGATCTGTAGCTATCACAGCCTTACTTTTACTCTTATCTTTACCCGTATTAGCAGGAGCCATTACTATATTACTTACAGATCGAAATTTAAATACAACCTTTTTTGACCCCGCAGGAGGAGGTGACCCTATCTTATACCAACATTTATTTTGATTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10353-scaled.jpg,",44.3704991188,-103.7530562011,06/20/2025,"
Caught from anthill near campgrounds.<\/p>\n",Lasius neoniger,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250715_211008663-scaled.jpg,","
Results are second row. Top: CO1. Bottom: Wol.<\/p>\n",No,ATCCGANTTAGAATTAGGCTCCTCGTGACTCACTAATTAATAATGATCAAATTTATAATTCTATAGTTACAAGACATGCATTCATTATAATCTTCTTTATAGTTATACCTTTTATAATTGGAGGGTTTGGTAACTTTCTTGTACCTTTAATATTAGGATCTCCTGATATAGCCTACCCTCGTATAAATAATATAAGATTCTGACTTTTACCCCCTTCAATTTCCCTACTCCTTTTAAGAAATTTTATTAATGATGGGGTTGGAACAGGATGGACTATTTACCCACCATTAGCTTCAAACATTTTTCACAATGGCCCTTCAGTTGACTTAACTATTTTTTCCCTTCATATTGCAGGGATATCTTCTATTTTAGGTGCTATTAATTTTATCTCAACTATTATAAATATACACCATAAAAATTTTTCTATTGATAAAGTACCCCTACTTGTATGATCAATTTTAATTACTGCAATTTTATTACTTTTATCTCTTCCTGTCCTTGCAGGAGCTATCACCATACTTTTAACTGATCGCAACCTTAATACCTCATTCTTTGACCCATCAGGAGGGGGAGATCCTATTTTATACCAACATCTTTTTTGATTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_3215-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_3218-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_3216-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_3217-scaled.jpeg,",42.459487,-73.314664,09/24/2025,"
The spider was outside on a web in the corner of a sidewalk.\u00a0 It looked white to the naked eye but under a microscope it was more brown-looking. It appears to be a garden ghost spider, also known as Hibana gracilis.<\/p>\n",Arthropoda,pcr,Abdomen,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_3416.jpeg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Fire-ant-WBP-rotated.jpg,",36.0170000000,-84.2620000000,10/15/2025,"",Solenopsis,DNeasy (Qiagen) blood and tissue kit.,Whole arthropod,MiniPCR,TBE,SeeGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Gel-WBP-1-rotated.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Ground-spider-scaled.jpg,",35.9858908000,-84.2805478000,10/15/2025,"",Euryopis funebris,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SeeGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/gel-electrophoresis-1-scaled.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/AJ1-CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/tree-spider-1.jpg,",35.9858908000,-84.2805478000,10/15/2025,"",Attulus pubescens,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SeeGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/gel-electrophoresis-2-scaled.jpg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/thumbnail_Resized_1000019405_1294430056475076.jpg,",36.0165809000,-84.2616673000,,"",Hemiptera,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,SeeGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/thumbnail_IMG_0811.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/thumbnail_Resized_1000019406_1294426366457230.jpg,",36.0165809000,-84.2616673000,,"",Arachnida,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,SeeGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/thumbnail_IMG_0811.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_6045-scaled.jpg,",42.4500845000,-73.2453824000,10/01/2025,"
It was just casually walking across first base on a baseball field. Once captured it hung from a thread in the middle of the vile.<\/p>\n",Philodromus rufus,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_6072-4.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_6072-2-1.png,","
Our first PCR test was set up on 10\/16\/2025 and was a duplex reaction that used both the Wolbachia 16S and Arthropod CO1 primers.\u00a0 The annealing temperature was 49 degrees C.<\/p>\n
We used a DNA extraction protocol based on the insect adaptation of New England Biolabs’ Monarch Spin gDNA Extraction kit (Product #T3010)<\/p>\n
The specimen was incubated for 30 minutes in a hot water bath at 56 degrees C<\/p>\n
Our first Gel image was taken on 10\/23\/2025:<\/p>\n
-was run at 125 volts for ~36 minutes<\/p>\n
-used this DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product #N0559S)<\/p>\n
Our second PCR reaction was set up on 10\/30\/2025, used the same Taq polymerase as the first PCR reaction, and:<\/p>\n
-was a single reaction that used the Wolbachia_F and Wolbachia_R primers<\/p>\n
-used an annealing temperature of 55 degrees Celsius<\/p>\n
Gel image was taken on 11\/6\/2025 and:<\/p>\n
-was run at 125 volts for ~21 minutes<\/p>\n
-used the DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product #N0559S)<\/p>\n",No,GTGCTTGGGCAGCAATAGTTGGTACAGCAATAAGTGTATTAATTCGAATAGAATTAGGACAAGTAGGTAAATTTTTAGGTGATGATCATTTGTATAATGTTATTGTTACTGCTCATGCATTTGTCATAATTTTTTTTATAGTAATACCTATTTTAATTGGTGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGTGCCCCAGATATAGCATTTCCTCGAATAAATAATTTAAGTTTTTGATTACTTCCTCCATCATTATTATTATTATTTATTTCTTCTATAGTTGAAATAGGAGTTGGTGCTGGTTGAACAGTTTATCCTCCTTTAGCATCTGCTACAGGTCATGCTGGAAGTGCTGTTGATTTTGCTATTTTTTCATTACATTTAGCAGGAGCATCTTCTATTATAGGTTCCATTAATTTTATTTCTACTATTGTTAATATACGATCAAGTGGTATAAAAATAGATAAAGTTCCGTTATTTGTATGATCTGTTATAATTACCACTGTATTATTACTTTTATCATTACCTGTATTAGCAGGTGCTATTACTATACTTTTAACAGATCGAAATTTTAATACTTCTTTCTTTGATCCTGCTGGAGGAGGAGATCCTATTTTATTTCAACATTTGTTTTGA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_5943-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_5944-scaled.jpeg,",41.9021431216,-71.4576189786,09/10/2025,"
\n
Found in grass field<\/li>\n
It jumped away when spooked even though it has wings<\/li>\n
Weather was partly cloudy<\/li>\n
Dry environment<\/li>\n<\/ul>\n",Fulgoridae,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,GelGreen,"","
N\/A<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_5946-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_5948-1-scaled.jpeg,",41.9020044918,-71.4576000000,,"
\n
Cloudy weather<\/li>\n
Found outside on concrete path near grass<\/li>\n
Missing a leg before capturing<\/li>\n<\/ul>\n",Opiliones,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,GelGreen,"","
N\/A<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_5945-scaled.jpeg,",41.9023258752,-71.4577585507,09/05/2025,"
\n
Found in the grass in open field<\/li>\n
Little activity in the tube–stood still most of the time<\/li>\n<\/ul>\n",Lepidoptera,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,GelGreen,"","
N\/A<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_5942-1-scaled.jpeg,",41.8841303442,-71.3848853721,09/05/2025,"
Found it in my concrete dry basement in my house<\/p>\n",Arthropoda,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,GelGreen,"","
N\/A<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_6041-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_6042-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_6044-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_6043-scaled.jpeg,",42.7112633000,-73.1994775000,10/01/2025,"
Was found drowning in Brown sugar & Yeast Trap (Alex Shen), was quickly preserved in 70% isopropyl alcohol<\/p>\n
Our first PCR reaction was set up on 10\/16\/2025 and:<\/p>\n
\n
was a duplex reaction because we used both the Arthropod CO1 and Wolbachia 16s primers together<\/li>\n
used an annealing temperature of 49 degrees Celsius.<\/li>\n
used this Taq polymerase: New England Biolabs OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer (#M0488S)<\/li>\n<\/ul>\n",Ravinia anxia,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_6072.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_6072-3.png,","
We used a DNA extraction protocol based on the insect adaptation of New England Biolabs’ Monarch Spin gDNA Extraction kit (Product # T3010)<\/p>\n
The specimen was incubated for 30 minutes in a hot water bath at 56 degrees C<\/p>\n
Our first Gel image was taken on 10\/23\/2025 and:<\/p>\n
\n
was run at 125 volts for ~36 minutes<\/li>\n
used this DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S)<\/li>\n<\/ul>\n
Our second PCR reaction was set up on 10\/30\/2025, used the same Taq polymerase as the first PCR reaction, and:<\/p>\n
\n
was a single reaction that used the Wolbachia_F and Wolbachia_R primers<\/li>\n
used an annealing temperature of 55 degrees Celsius<\/li>\n<\/ul>\n
Gel image was taken on 10\/6\/2025 and:<\/p>\n
\n
was run at 125 volts for ~21 minutes<\/li>\n
used this DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (Product # N0559S)<\/li>\n<\/ul>\n",No,TGATCAGGAATAGTAGGAACTTCTTTAAGAATTCTTATTCGAGCAGAATTAGGACATCCAGGAGCATTAATTGGAGATGACCAAATTTATAATGTTATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATGCCAATTATAATTGGAGGATTCGGAAATTGATTAGTTCCTATTATACTTGGTGCTCCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTTTGATTACTACCCCCTGCATTAACATTACTACTAGTAAGTAGTATAGTAGAAAACGGAGCTGGAACAGGATGAACTGTATACCCTCCTTTATCATCTAATATTGCTCATGGAGGAGCCTCTGTTGATTTAGCTATTTTTTCTCTACATTTAGCAGGGATTTCATCAATTTTAGGGGCAGTAAATTTTATTACTACAGTTATTAATATACGATCAACAGGAATTACTTTTGACCGAATACCTCTATTTGTTTGATCTGTAGTAATTACTGCCTTATTATTACTTTTATCTTTACCAGTTCTTGCTGGAGCAATTACTATACTTTTAACTGATCGAAATATTAATACTTCATTTTTTGATCCAGCAGGAGGAGGAGACCCTATTTTATACCAACATTTATTTTGA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0293-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0288-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0294-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0292-scaled.jpeg,",42.4594870000,-73.3146640000,10/01/2025,"
It was extracting polen with the rest of bees, it is very fluffy under the microscope.<\/p>\n
Has wings and has 6 legs.<\/p>\n",Bombus impatiens,Monarch DNA extraction (NEB),Abdomen,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_0914-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DNA-Ladder.png.png,","
We followed throughly the steps to perform the gel electrophoresis and actually gave us valid results, on the pictures you can appreciate the labels<\/p>\n",No,GAGCAGGAATAGTAGGAACATCATTAAGAATATTAATTCGAGTAGAATTAGGTCATCCAGGAACATTTATTGGAAATGATCAAATTTATAATGTAATTGTTACAGCACATGCATTTATCATAATTTTTTTTATGGTAATACCAATTATAATTGGAGGATTTGGAAATTGACTAGTACCCCTAATACTAGGAGCCCCTGATATAGCCTTTCCCCGAATAAATAATATAAGTTTTTGATTATTACCACCTTCCCTTACATTATTATTAATAAGAAGAATAGTAGAAAGAGGAGCCGGTACAGGGTGAACAGTATACCCGCCACTCTCATCCACTATTGCTCATAGAGGAGCATCAGTTGATTTAGCAATTTTTAGATTACATCTAGCTGGAATTTCTTCTATTTTAGGAGCCGTAAATTTTATTACTACAGTTATTAATATACGATCCCCTGGGATTACTCTAGATCGAATGCCTTTATTTGTTTGATCAGTAGTAATTACTGCACTTTTACTTCTATTGTCTTTACCTGTTCTAGCCGGAGCAATCACAATATTATTAACTGATCGTAATTTAAATACTTCATTCTTTGACCCAGCGGGAGGAGGAGATCCAATTTTATACCAACATCTATTTTGA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10337-scaled.jpg,",43.49629294760531,-111.96111984732973,06/16/2025,"
Caught live near flower bed.<\/p>\n",Sciaridae,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250708_1910424612-scaled.jpg,","
Results are ninth row. Top: CO1. Bottom: Wol.<\/p>\n",No,CAAGAATAGTTGGAACCTCATTAAGAATATTAATTCGGGCTGAGCTTGGATGTCCAAATGCTTTAATTGGAGATGACCAAATTTATAATGTTATTGTCACTGCTCATGCATTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAACTGATTAGTTCCTTTAATATTAAGAGCCCCAGATATAGCTTTCCCACGATTAAATAATATAAGATTTTGATTGCTCCCCCCTTCTCTTACATTATTATTAACAAGAAGTTTAGTTGAAAGAGGAACAGGAACCGGATGAACGGTTTATCCTCCTCTTTCTTCTAATATTTCTCATTCAGGAGCATCTGTTGATTTATCAATTTTTTCACTTCATTTAGCTGGAATTTCATCAATTTTAGGAGCTGTAAATTTTATTTCAACTATTATTAATATACGAGCCCCCGGGATATCATTTGACAAGATACCATTATTTGTTTGATCTGTCTTAATTACAGCAATTTTATTATTACTCTCATTACCAGTTTTAGCTGGAGCAATTACCATATTACTTACAGACCGTAATTTAAATACTTCATTTTTTGATCCAGCAGGAGGAGGGGATCCTATTTTATANNNACATTTATTCTGAT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10336-scaled.jpg,",43.49630073050531,-111.96113057616579,06/16/2025,"
Collected live from front walkway.<\/p>\n",Tetramorium caespitum,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250708_1910424612-scaled.jpg,","
Results are eighth row. Top: CO1. Bottom: Wol.<\/p>\n",No,GAGCAGGAATAATCGGATCTTCAATAAGTATAATTATCCGACTTGAATTAAGTTCCTGTGGTTCATTAATTAATGACGACCAAGTATATAATTCCTTAGTAACAAGACATGCCCTTGTTATAATTTTCTTTATAGTAATGCCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCCATTAATACTAGGAACTCCCGATATAGCCTACCCCCGTATAAATAACATAAGATTTTGACTTCTACCACCCTCTATTAGATTATTATTGCTTAAAAGATTTATTAATCCAGGGGTAGGAACAGGATGAACTATCTATCCCCCATTAGCATCTAATATCTTTCATAGAGGCCCATCAATTGATCTATCAATTTTTTCTCTTCATATTGCAGGAATATCTTCTATTATAGGTGCAATTAATTTTATCGCTACTATTATAAACATACATCATAAAAAATTAACCTTAGATAAAATCCCCCTGTTAGTTTGATCTATTTTAATTACAGCAATTCTTCTCCTCCTATCTCTTCCCGTATTAGCAGGAGCTATTACTATACTCTTAACAGATCGTAATTTAAATACCTCATTTTTTGATCCATCAGGTGGGGGGGACCCAATTCTATATCAACATTTATTCTGA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10335-scaled.jpg,",43.49630073050531,-111.96113057616579,06/16/2025,"
Collected live from flower bed.<\/p>\n",Armadillidium vulgare,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250708_1910424612-scaled.jpg,","
Results are seventh row. Top: CO1. Bottom: Wol.<\/p>\n",No,GAGCAGGGGCTGTTGGGACTGCCCTTAGAATAATTATTCGCACTGAATTAGGACAGCCTGGAAGTTTAATTGGAGATGATCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATACCTATTATAATTGGTGGATTCGGTAACTGGTTAATTCCATTAATACTAGGAGCCCCAGATATAGCTTTTCCACGGATAAATAATATAAGATTTTGACTTTTACCCCCTTCTTTAACTTTATTACTTAGAAGGGGATTAGTTGAAAGAGGAGTAGGAACAGGGTGGACAGTATATCCTCCGCTGGCGTCGAGAATTGCTCACAGAGGTGCATCTGTAGATTTAGGTATTTTTTCTCTCCACCTTGCTGGAGCTTCTTCTATTTTAGGGGCTGTAAATTTTATTACTACTATAATTAATATACGAGCAGCTGGAATCAGAATAGACCGTGTTCCTTTATTTGTTTGATCAGTAATAGTAACGGCTGTGCTTTTGCTTTTATCATTACCTGTACTAGCAGGAGCTATTACTATATTATTAACTGATCGAAATTTAAATACCTCTTTTTTTGACCCTAGCGGAGGTGGGGATCCTATCCTTTATCAACATTTATTCTGATT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10333-scaled.jpg,",43.4963007305,-111.9611211356,06/16/2025,"
Collected live from lawn.<\/p>\n",Doratura stylata,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250708_1910424612-scaled.jpg,","
Results are fifth row. Top: CO1. Bottom: Wol.<\/p>\n",No,GGGAATAATACTAAGAATAATTATTCGGATTGAATTAGCTCAACCAGGGTCAGTTATTAATAATGATCAAGCATATAATGTAATTGTAACTTCACATGCTTTCATTATAATTTTCTTTATAGTTATACCAATTATAATTGGAGGATTTGGTAATTGACTTTTACCTTTAATAATTGGTGCTCCTGATATAGCATTTCCTCGTCTTAACAATATAAGATTTTGATTACTTCCACCTTCATTATGTTTACTAATCATGAGTTCTATAATCGAAATAGGAACAGGAACAGGATGAACGGTATACCCACCTCTGTCCAGAAATATAGCACATGCAGGGCCAAGAGTAGATTTATCAATCTTTTCTCTACACTTGGCTGGAATTTCATCAATTTTAGGTGCAGTAAACTTTATTACAACAGTAATTAATATACGACCTGAGGGAATATCTATAGACCGAACACCATTATTTGTATGGTCAGTTCTTATTACTGCAATCTTACTACTTTTATCTTTACCAGTTTTAGCAGGAGCAATTACTATACTTTTAACAGATCGAAATATCAACACAACGTTTTTTGACCCAGCTGGTGGGGGGGACCCTATCTTGTACCAACATCTATTCTGATTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10332-scaled.jpg,",43.496306698075465,-111.96112248676224,06/16/2025,"
Collected inside living room.<\/p>\n",Fannia canicularis,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250708_1910424612-scaled.jpg,","
Results are fourth row. Top: CO1. Bottom: Wol.<\/p>\n",No,GATCTGGTATAGTAGGGACATCTTTAAGTATTCTAATTCGAGCTGAATTAGGTCATCCTGGAGCTTTAATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCTATTATAATTGGTGGATTTGGTAATTGATTAGTACCTTTAATATTAGGAGCTCCTGATATAGCATTTCCTCGAATAAATAATATAAGATTTTGATTATTACCTCCAGCTTTAACTTTATTACTAGTAAGAAGTATAGTAGAAAATGGAGCTGGTACTGGTTGAACTGTTTACCCTCCACTTTCATCTAACATTGCTCATAGAGGAGCTTCAGTTGATTTAGCAATTTTCTCTCTTCATCTCGCAGGTATTTCATCTATTTTAGGAGCTGTAAATTTTATTACAACTGTAATTAATATACGATCTACTGGAATTACATTTGATCGAATACCTTTATTTGTTTGATCTGTAGTTATTACAGCTTTATTATTACTTTTATCTTTACCAGTATTAGCTGGAGCTATTACTATATTATTAACAGATCGAAATCTTAATACCTCATTCTTTGACCCTGCAGGAGGTGGAGATCCTATCCTTTATCAACATTTA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10331-scaled.jpg,",43.492660199999996,-111.98441653333296,06/13/2025,"
Collected live in kitchen.<\/p>\n",Chironomidae,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250708_1910424612-scaled.jpg,","
Results are third row. Top: CO1. Bottom: Wol.<\/p>\n",No,GCTTGATCTGGAATAGTAGGTACTTCCCTTAGTATCTTAATTCGAGCAGAACTAGGACATGTCGGATCTTTAATTGGAGACGACCAAATTTACAACGTAATTGTTACTGCCCATGCTTTTGTTATAATTTTTTTTATAGTAATACCAATTTTAATTGGTGGTTTTGGAAACTGACTTGTACCCCTTATACTCGGTGCCCCTGATATAGCTTTCCCCCGAATAAATAATATAAGATTTTGACTATTACCCCCTTCTTTAACTTTACTTTTATCTAGATCTATTGTTGAAAATGGGGCTGGAACAGGATGAACTGTTTATCCTCCTCTTTCTTCAGGAATTGCTCATGCTGGAGCTTCTGTTGATTTAGCTATTTTTTCTTTACATTTAGCAGGTATTTCTTCAATTTTAGGGGCAGTAAATTTTATTACGACAGTAATTAATATACGTTCAGAAGGAATTACTTTAGATCGAATACCTTTATTTGTCTGATCAGTAATTATTACAGCAATCTTACTTTTATTATCTTTACCAGTCCTTGCTGGAGCTATTACTATGTTACTTACAGATCGTAATTTAAATACTTCTTTCTTTGACCCTGCCGGAGGGGGAGACCCCATTCTTTACCAACACTTATTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10314-scaled.jpg,",43.4926991168,-111.9844134819,06/12/2025,"
Collected live from a flowering almond.<\/p>\n",Araneus gemmoides,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250701_1931453292-scaled.jpg,","
Results are fifth row. Top: CO1. Bottom: Wol.<\/p>\n",No,TTGAGCAGCTATAGTAGGAACAGCAATAAGTGTATTAATTCGAATTGAATTAGGTCAACCTGGTAGATTTATAGGGGATGATCAACTTTATAATGTAATTGTGACTGCACATGCATTTGTAATAATTTTTTTTATAGTGATGCCAATTTTAATTGGCGGTTTTGGAAATTGATTAGTTCCTTTGATATTAGGTGCACCTGATATAGCATTTCCTCGAATAAATAATTTAAGATTTTGATTACTTCCCCCTTCTTTATTTCTTTTGATTGTTTCTTCTATAGTTGAAATGGGTGTAGGTGCTGGTTGGACTGTATATCCTCCTTTAGCTGGTCTAGAAGGGCATGCTGGGAGGTCTGTAGATTTTGCAATTTTTTCTTTACATTTAGCTGGGGCTTCATCTATCATGGGTGCAATTAATTTTATTTCTACAATTATTAATATGCGATTTTATGGTATAACAATAGAAAAGGTTCCTTTATTTGTATGATCAGTATTGATTACAGCTGTTTTATTATTACTTTCTTTACCTGTATTAGCAGGGGCAATTACTATATTATTAACTGATCGTAATTTTAATACATCTTTTTTTGATCCTTCCGGAGGGGGAGATCCAATTTTATTTCAACATTTGTTTTGATTTTTTG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10313-scaled.jpg,",43.4926840591,-111.9844271386,06/12/2025,"
Collected live from a honeysuckle.<\/p>\n",Hyadaphis passerinii,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250701_1931453292-scaled.jpg,","
Results are fourth row. Top: CO1. Bottom: Wol.<\/p>\n",No,GATCNGGTATAATTGGTTCATCTCTTAGAATTTTAATTCGTTTAGAATTAAGACAAATTAACTCTATTATTAATAATAATCAATTATATAATGTAATTGTTACAATTCATGCCTTTATTATAATTTTTTTCATAACAATACCAATTGTTATTGGTGGATTTGGAAATTGATTAATTCCTTTAATAATAGGATGTCCTGATATATCTTTTCCACGATTAAATAATATTAGATTTTGATTATTACCTCCATCATTAATAATAATAATTTGTAGCTTTATAATTAATAATGGAACAGGAACAGGATGAACAATTTACCCACCATTATCAAATAATATTGCACATAATAATATTTCAGTAGATTTAACTATTTTTTCTTTACACTTAGCAGGAATTTCATCAATTTTAGGAGCAATTAATTTTATTTGCACAATTCTTAATATAATACCTAATAATATAAAAATTAATCAAATTCCTCTTTTCCCATGATCAATCCTAATTACAGCTATTTTATTAATTTTATCTCTACCAGTTTTAGCTGGTGCTATTACAATATTATTAACTGATCGTAATTTAAATACTTCATTTTTTGATCCAGCAGGAGGAGGAGATCCAATTTTATATCAACATTTATTTTGATTTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10312-scaled.jpg,",43.49269133346686,-111.98441132808465,06/11/2025,"
Collected dead from porch.<\/p>\n",Forficula auricularia,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250701_1931453292-scaled.jpg,","
Results are third row. Top: CO1. Bottom: Wol.<\/p>\n",No,GGGANAGTAGGGACTTCATTGAGTTTGTTGATTCGAGCAGAGTTGGGACAACCTGGAGCTTTAATTGGAGACGATCAAATTTATAATGTAATTGTTACGGCCCACGCTTTCGTAATGATTTTTTTTATGGTAATGCCTATTATGATTGGGGGTTTTGGGAATTGATTGGTACCCCTGATGCTGAGAGCCCCTGACATGGCCTTTCCTCGAATAAATAATATAAGTTTTTGGTTACTACCTCCTTCGCTTATACTTTTGCTCTCTGGGAGAATGGTAGACAGAGGGGCAGGAACGGGTTGGACGGTCTACCCTCCCCTGTCGGGGGCTATCGCTCACGCAGGGGCTTCGGTGGATTTGAGAATTTTTTCATTGCACTTGGCGGGGGTTTCATCCATTTTAGGGGCAATTAATTTTATTACAACGGTGATTAACATGCGCCCCTCAGGTCTTAAATTGGAACGAATACCATTATTTGTGTGATCTGTAGCCATTACTGCTCTTTTATTGTTACTTTCTTTACCAGTGTTGGCAGGGGCTATCACTATGCTTTTGACAGACCGGAATTTAAATACATCTTTTTTTGACCCTGCGGGAGGGGGAGACCCTATTCTCTATCAACATTTATTTTGATTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/DSC10311-scaled.jpg,",43.49271059268895,-111.98442252141723,06/11/2025,"
Collected dead from porch.<\/p>\n",Reduvius personatus,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/PXL_20250701_1931453292-scaled.jpg,","
Results are second row. Top: CO1. Bottom: Wol.<\/p>\n",No,GGAACATCGCTCAGATGATTAATTCGAATTGAATTAGGACAACCAGGATCCTTCATCGGTGATGACCAAACATATAATGTTATAGTTACTGCACATGCATTCATTATGATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTTGGGAACTGATTAGTACCCTTAATGATTGGGGCCCCAGATATAGCTTTCCCACGAATAAATAATATAAGTTTTTGATTATTACCCCCATCTCTCACTCTCTTAATTATAAGAAGTATTGTAGAAACAGGGGCAGGAACAGGATGAACCGTTTACCCCCCTCTATCAAGAAATATGAGACATGCAGGTGCATCCGTTGACCTTGCAATTTTTTCTCTTCACCTAGCAGGGATTTCATCAATCTTAGGAGCAGTAAATTTTATTTCAACCATCATCAATATACGAACCGCGGGAATAACCCCCGAACGGATCCCTCTTTTTGTTTGATCAGTTGGTATTACTGCACTTCTCCTACTTCTCTCTTTACCTGTCCTAGCAGGAGCAATTACTATACTCCTAACAGATCGAAACTTTAATACAAGATTTTTTGACCCAGCTGGAGGGGGGGATCCTATTTTATATCAACATTTATTTTGATTTTTTGGG,,, "",,,,"",Diptera,,,,,,"","",,,,, "",43.5916887000,-70.3371881000,,"",,DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL,Abdomen,MiniPCR,TBE,GelGreen,"","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-26-1.51.47-PM.png,",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-12-2.13.47-PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-12-2.13.43-PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-12-2.13.38-PM.png,",43.5917469923,-70.3376410728,10/03/2024,"",Ocypus olens,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-09-1.44.11-PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-09-1.44.17-PM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_1953-scaled.jpg,",43.5916887000,-70.3371881000,09/21/2024,"
long legs, arachnid body, small head, got it inside of a classroom,<\/p>\n",Pholcidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/2.jpg,",38.9289839287,-77.0698150116,09/11/2025,"
I caught the mosquito around noon and it was around 74 F outside. I caught it in a wooded area and there were a lot of mosquitos around.<\/p>\n",Aedes albopictus,Blood and Tissue kit,Whole arthropod,MiniPCR,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/9_19GEL_1.jpg,","",Yes,,,, "",,,09/17/2025,"
On a tree near a building, 70 degrees, partly cloudy, blackish red color<\/p>\n",Camponotus pennsylvanicus,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SeeGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-09-135925.png,","
Lanes 2 and 3 were my lanes for testing<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_5432.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_5435.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_5436.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_5438.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_5440-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_5442-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_5443-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_5444-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_5445-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_5446-scaled.jpg,",42.4591901000,-73.3156010000,10/01/2025,"
It has a head, thorax, and abdomen, characterized by black and yellow coloration. The head and thorax are covered with yellow fur, while the abdomen is black. It also has two large compound eyes, four wings, and antennae.<\/p>\n",Bombus impatiens,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_5526-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Gel-3.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Gel-4.jpg,","
We used a DNA extraction protocol based on the insect adaptation of New England Biolabs’ Monarch Spin gDNA Extraction kit (Product # T3010). The specimen was incubated for 40minutes in a hot water bath at 56 degrees C.<\/p>\n
Our first PCR reaction was set up on 10\/14\/2025 and:<\/p>\n
\n
was a duplex reaction because we used both the Arthropod CO1 and Wolbachia 16S primers together.<\/li>\n
used an annealing temperature of\u00a0 \u00a049 \u00a0degrees Celsius.<\/li>\n
used this Taq polymerase: New England Biolabs OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer (#M0488S<\/li>\n
Our first Gel image was taken on 10\/22\/25 and:\n
\n
was run at 125volts for 24 minutes<\/li>\n
used this DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n
\n
The second PCR reaction was set up on 10\/29\/25, used the same Taq polymerase as the first PCR reaction, and:<\/li>\n
was a single PCR reaction that used the Wolbachia 16S F and 16S R primer(s).<\/li>\n<\/ul>\n
\n
used an annealing temperature of\u00a0 55 degrees Celsius.<\/li>\n
Our second Gel image was taken on 11\/5\/2025 (date) and:\n
\n
was run at 125 volts for 22 minutes<\/li>\n
used this DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S)<\/li>\n<\/ul>\n<\/li>\n
<\/li>\n<\/ul>\n",Yes,TAATCGGATCATCAATAAGATTATTAATTCGAATAGAACTTAGACATCCAGGAATATGAATTAATAATGATCAAATTTATAATTCATTAGTTACAAGACATGCATTTTTAATAATTTTTTTTATAGTTATACCATTTATAATTGGAGGATTCGGAAATTATTTAATTCCTTTAATATTAGGATCACCTGATATAGCTTTTCCACGAATAAATAATATTAGATTTTGATTATTACCTCCATCTATTTTTATATTATTATTAAGAACTTTATTTACACCTAATGTAGGAACAGGTTGAACCGTATATCCCCCTTTATCATCTTATTTATTTCATTCATCACCTTCAGTAGATATTGCAATTTTTTCTTTACATGTAACAGGAATTTCTTCTATTATTGGTTCATTAAATTTTATCGTTGCTATTATATTAATAAAAAATTTTTCATTAAATTATGATCAAATTACTTTATTTTCATGATCTGTATGTATTACAGTATTATTATTAATTTTATCATTACCAGTTTTAGCAGGAGCAATTACTATACTTCTTTTTGATCGAAATTTTAATACATCATTTTTTGATCCAATAGGAGGTGGAGATCCAATTTTATATCAACATTTATTTTGA,,, "",44.9330076000,-93.1629063000,09/17/2025,"
Found outside the school, and social near a group of other ants. Was small compared to other ants.<\/p>\n",Formica glacialis,DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL,Abdomen,agarose gel electrophoresis,TBE,SeaGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-09-082321.png,","",Yes,,,, "",44.9282147610,-93.1690586542,09/17/2025,"
My fly was very big and found in my friends house. Fly is a solitary bug.<\/p>\n",Calliphora vicina,Qiagen DNeasy Blood and Tissue Kit,Abdomen,MiniPCR,TBE,SeeGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-09-082330.png,","
Lanes 6 and 7.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/1000012345-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/1000012349-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/1000012351-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/1000012352.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/1000012343-scaled.jpg,",42.4732700000,-73.2453824000,09/29/2025,"
\n
The specimen been caught under a 5 foot long rock, under moist dirt in a garden flower.\u00a0 Only odd thing that there was two stone centipedes that were around 8 inches near each other.<\/li>\n<\/ul>\n",Lithobius forficatus,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/1000012954.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/1000012951-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/1000012952-scaled.jpg,","
We used the DNA extraction protocol based on insect adaptation of new england’s biolabs monarch spin gDNA Extraction kit. (Product # T3010) Specimen was incubated for 15 minutes in a hot water bath at 56 degrees C.<\/span><\/p>\n
First pcr was done on oct 14th 2025 and was a duplex reaction since both arthropod CO1 and wolbachia 165 prime together. Annealing temperature at 49 degrees Celsius. Used Taq polymerase new england biolabs hot start quick-load 2x master with standard buffer (M0488S) Our first gel was taken on 10\/21\/2025 and ran at 100 \u00a0 Volts for 25 minutes and <\/span>Used DNA ladder:new england biolabs 1kb plus DNA ladder for safe stains (product # No559s)<\/span><\/p>\n
Our third\u00a0 PCR was a single\u00a0 PCR that only had Woolbachia primers using the same equipment. Our third\u00a0 gel image was taken on 11\/4 was run at 125 volts for 25 minutes used DNA ladder: new england biolab 1kb plus DNA ladder safe for stains (product # No559s)\u00a0<\/span><\/p>\n",No,GATCAGCAATAATTGGGACTGGCCTTAGCCTTCTTATTCGGTTAGAGTTAAGACAGCCTGGAAGATTAATTGGAGATGATCAAATTTATAATGTCATTGTTACCGCTCACGCCTTCATTATAATTTTTTTTATAGTTATACCAATCATAATTGGGGGATTCGGAAATTGACTTGTACCCTTAATATTAGGGGCCCCAGACATAGCATTTCCTCGAATAAATAACATAAGATTTTGATTACTCCCACCCTCCCTAACTCTCTTACTGTGTTCAGCAGCAGTAGAAAGAGGGGCAGGAACGGGGTGAACCGTTTACCCTCCCTTATCTTCTAATATTTCTCATAGAGGAGCATCAGTTGATATAACAATCTTTTCCCTACATCTCGCCGGAGCATCATCAATCCTGGGAGCAATCAACTTTATCTCCACTATTATTAATATACGGACTAGAGGAATGTCATTTGAACGAGTCCCCCTATTTGTGTGATCCGTAAAAATTACAGTTATCTTGCTCCTCCTTTCTCTTCCAGTATTAGCCGGAGCAATTACAATATTATTAACTGACCGAAACTTAAATACTAGCTTCTTCGACCCTACAGGAGGGGGAGACCCCATTTTATACCAACATTTATTTTGA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-175-scaled.jpg,",41.9295355,-88.74991279999999,10/17/2024,"
spiderara<\/p>\n",Araneae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-165-scaled.jpg,",41.9295355,-88.74991279999999,10/17/2024,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-164-scaled.jpg,",41.9295355,-88.74991279999999,10/17/2024,"
earwig<\/p>\n",Dermaptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-163-scaled.jpg,",41.9295355,-88.74991279999999,10/17/2024,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-162-scaled.jpg,",41.9295355,-88.74991279999999,10/17/2024,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-158-scaled.jpg,",41.9295355,-88.74991279999999,10/17/2024,"
soldier beetle<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-157-scaled.jpg,",41.9295355,-88.74991279999999,10/17/2024,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-156-scaled.jpg,",41.9295355,-88.74991279999999,10/17/2024,"
wasp<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-155-scaled.jpg,",41.9295355,-88.74991279999999,10/17/2024,"
picnic beetle<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-154-scaled.jpg,",41.9295355,-88.74991279999999,08/14/2024,"
rolly polly<\/p>\n",Isopoda,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-153-scaled.jpg,",41.9295355,-88.74991279999999,08/14/2024,"
rolly polly<\/p>\n",Isopoda,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-151-scaled.jpg,",41.9295355,-88.74991279999999,09/19/2024,"
wasp<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-150-scaled.jpg,",41.9295355,-88.74991279999999,08/12/2024,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-149-scaled.jpg,",41.9295355,-88.74991279999999,09/05/2024,"
millipede<\/p>\n",Polydesmida,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-148-scaled.jpg,",41.9295355,-88.74991279999999,09/05/2024,"
millipede<\/p>\n",Polydesmida,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-147-scaled.jpg,",41.9295355,-88.74991279999999,09/05/2024,"
millipede<\/p>\n",Polydesmida,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-146-scaled.jpg,",41.9295355,-88.74991279999999,09/26/2024,"
millipede<\/p>\n",Polydesmida,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-145-scaled.jpg,",41.9295355,-88.74991279999999,09/12/2024,"
milliepede<\/p>\n",Polydesmida,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-141-scaled.jpg,",41.9295355,-88.74991279999999,09/25/2024,"
millipede<\/p>\n",Polydesmida,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/tempImageEBUIao.jpg,",40.5592069935,-75.4025478152,09/11/2025,"
The yellow jacket was curious\/aggressive in its natural environment. The yellow jacket shows black and yellow bands along the abdomen with thick, even stripes, and black dots. It has a black antennae and clear wings with black\/brown veins. With the overall body shape being short and lean along with the continuous patterns I have determined this to be an eastern yellow jacket rather than a paper wasp or hornet.<\/p>\n",Vespula maculifrons,pcr,Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/tempImageDjJzPL-scaled.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_8644-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_8645-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_8641.jpeg,",42.6951614484,-73.0353203094,09/27/2025,"
Black beetle but has a brown stripe in the center of the body. Appears to have wings as well; very small.<\/p>\n",Dermestes lardarius,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/image-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_8817.jpeg,","
We used a DNA extraction protocol based on the insect adaptation of New England Biolabs\u2019 Monarch Spin gDNA extraction kit (Product #T3010). The specimen was incubated for 30 minutes in a hot water bath at 56 degrees C.<\/p>\n
Our first PCR reaction was set up on 10\/14\/25 and was a duplex reaction because we used both the Arthropod CO1 and Wolbachia 16S primers together. We used an annealing temperature of 49 degrees Celsius. Our Taq polymerase was New England Biolabs OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer (#M0488S).<\/p>\n
Our first gel image was taken on 10-21-25 and was run at 125 volts for 15 minutes. We used the New England Biolabs 1 kb plus DNA ladder for safe stains (product # N0559S)<\/p>\n
Our second PCR reaction was set up on 10-28-25. We used the same Taq polymerase as first reaction. I did a single reaction that used Wolbachia_F and Wolbachia_R primers. I used an annealing temperature of 55 degrees Celsius.<\/p>\n
Our second gel image was taken on 11-4-25 and it was run at 125 volts for 30 minutes. We used the New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S).<\/p>\n",Yes,TGAGCAGGAATAGTAGGAACATCCTTAAGAATACTAATTCGAACAGAATTAGGAATACCTGGCTCATTAATTGGAAACGATCAAATTTTTAATGTAATCGTTACAGCACATGCATTTATCATAATTTTCTTTATAGTAATGCCCATTATAATTGGAGGATTTGGAAATTGACTTGTCCCATTAATACTTGGAGCACCAGATATAGCATTCCCCCGAATAAATAACATAAGATTTTGACTTCTACCTCCTTCACTATCTTTATTACTGATAAGAAGAATAGTAGAAAGAGGAGCAGGAACAGGGTGAACAGTATACCCTCCATTATCTGCAAATATTGCCCATAGAGGAGCTTCAGTAGACCTAGCTATTTTTAGATTACATTTAGCAGGAATTTCTTCTATTCTTGGAGCAGTAAATTTTATTACAACTGTTATTAATATACGATCAAAAGGAATAACTCCTGACCGAATACCACTATTCGTTTGATCAGTAGCTATTACTGCACTTCTTCTTCTTTTATCATTACCTGTTCTAGCTGGAGCAATCACTATACTTCTCACAGATCGAAATCTTAATACTTCATTTTTTGATCCAGCAGGAGGAGGTGATCCTATTCTTTATCAACATTTATTTTGA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/1000015231-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/1000015233-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/1000015232-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/1000015223.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/1000015224.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/1000015221.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/1000015229.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/1000015230.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/1000015227.jpg,",42.4604820000,-73.1761210000,09/23/2025,"
The arthropod was found on top of a mostly still pool of water at the edge of a brook.\u00a0 It was skidding across the water with others of the same species.\u00a0 It was collected just after dark on a day that had light rain throughout the afternoon.\u00a0 The temperature at the time of collection was about 17.8 degrees C (64 degrees F).<\/p>\n
<\/p>\n
*Note that the arthropod was identified as Chironomidae sp. <\/span><\/i>by NCBI BLAST using its DNA sequence (from the CO1 gene), but that identification was not considered accurate based on the observations of the collected arthropod.\u00a0 Body structure and behaviors before collection did not line up with those expected from Chironomidae sp.<\/i>.\u00a0 Based on observations the arthropod is deemed to be a water strider of an undetermined species (Gerris sp.).<\/em>*<\/span><\/p>\n",Gerris,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Screenshot_23-10-2025_14043_docs.google.com_.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot_4-11-2025_132928_docs.google.com_.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot_4-11-2025_133025_docs.google.com_.jpeg,","
\n
We used a DNA extraction protocol based on the insect adaptation of New England Biolabs’ Monarch Spin gDNA Extraction kit (Product # T3010).<\/li>\n
The specimen was incubated for 30 minutes in a hot water bath at 56 degrees C.<\/li>\n<\/ul>\n
<\/p>\n
\n
Our first PCR reaction was set up on 10\/14\/25 and was a duplex reaction because we used both the Arthropod CO1 and Wolbachia 16S primers together.<\/li>\n
We used an annealing temperature of 49 degrees Celsius.<\/li>\n
We used this Taq polymerase: New England Biolabs One Taq Hot Start Quick-Load 2X Master Mix with Standard Buffer (#M0488S)<\/li>\n
Differed from the original protocol in that: We used 88uL of Taq Master Mix in our PCR Cocktail rather than 87.5uL.<\/li>\n<\/ul>\n
<\/p>\n
\n
Our first Gel image was taken on 10\/21\/25 and was run at 125 volts for 13 minutes and used this DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S)<\/li>\n<\/ul>\n
<\/p>\n
\n
Our second PCR was set up on 10\/28\/25, used the same Taq polymerase as the first PCR reaction, and was a single reaction that used Wolbachia 16S rRNA primers.<\/li>\n
We used an annealing temperature of 55 degrees Celsius.<\/li>\n
It differed from the original protocell in that: we used 46uL of water and 88uL of Taq Master Mix in our PCR cocktail rather than 45.5uL and 87.5uL (respectively).<\/li>\n<\/ul>\n
<\/p>\n
\n
Our third PCR was set up on 10\/28\/25, used the same Taq polymerase as the first PCR reaction, and was a single reaction that used Arthropod CO1 primers.<\/li>\n
We used an annealing temperature of 49 degrees Celsius.<\/li>\n
It differed from the original protocell in that:<\/li>\n
1. We used only one collected arthropod sample (1-KM), and only the +DNA and water for controls.<\/li>\n
2. We mixed a PCR cocktail using 8uL Arthropod_F Primer, 8uL Arthropod_R Primer, 26uL water, and 50uL Taq Master Mix 2x (enough for 4 total reactions).<\/li>\n<\/ul>\n
<\/p>\n
\n
Our second Gel image was taken on 11\/4\/25 and was run at 125 volts for 25 minutes.\u00a0 We used this DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S)<\/li>\n<\/ul>\n
<\/p>\n
\n
Our third Gel image was taken on 11\/4\/25 and was run at 125 volts for 25 minutes.\u00a0 We used this DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S)<\/li>\n<\/ul>\n",Yes,TTATTTTTTTGGAGCTTGATCTGGAATAGTAGGAACTTCCCTAAGTATACTTATTCGAGCAGAATTAGGCCATCCTGGCACATTTATTGGAGATGACCAAATTTATAATGTTATTGTAACAGCTCATGCTTTCATTATAATTTTTTTTATAGTTATACCTATTTTAATTGGAGGTTTTGGTAATTGACTTTTACCTTTAATACTAGGAGCCCCTGATATAGCATTCCCACGAATAAATAATATAAGTTTTTGATTATTACCCCCTTCTCTTTCTTTACTTCTTTCTAAAAGAATTGTAAAAAATGGTGCAGGAACTGGATGAACAGTTTATCCACCTTTATCTTCCAAAATTGCACATAGAGGGGCTTCAGTCAATTTAGCTATCTTTTCTCTTCATCTAGCCGGAATTTCTTCTATTCTTGGGTCTGTAAATTTTATTACAACAGCTATCAATATACAATCAAATGGAATCTCATTAAATCGAATACCATTATTTGTATGATCTGAAATTATTACAACAATCTTACTACTTATTTCTCTTCCTGTATTAGCCGGAGCCATTACTATACTTTTAACAAACCGAAATTTAAATACATCTTTCTTTGACCCTGCAGGAGGAGGAAACCCAATTTTATACCAACATCTATTTTGATTTTTTGGCCCCCCGGAAGTATTAAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/tempImage0IP8CN-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/tempImageNbB5yw-scaled.jpg,",42.4500845000,-73.2453824000,09/29/2025,"
\n
Next to a garden<\/li>\n
Outside open area<\/li>\n
Crawling out from underground<\/li>\n<\/ul>\n",Lasius neoniger,Monarch DNA extraction (NEB),Whole arthropod,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Samara_gel.png,","
1. We used a DNA extraction protocol based on the insect adaptation of New England Biolabs\u2019 Monarch Spin gDNA Extraction kit (Product #T3010)<\/p>\n
2. The specimen was incubated for 30 minutes in a hot water bath at 56 degrees C.<\/p>\n
3. Our first PCR reaction was set up on October 14th,2025 and was a duplex reaction because we used both the Arthropod CO1 and Wolbachia 16s primers together<\/p>\n
4. used an annealing temperature of 49 degrees Celsius<\/p>\n
5. used this Taq polymerase: New England Biolabs OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer (#M0488S)<\/p>\n
6. differed from the written protocol (time differences, accidental mistakes, etc.)<\/p>\n
7. Our first Gel Image was taken on 10\/21\/25 – was run at 125 volts for 20 minutes<\/p>\n
– used this DNA ladder: New England Biolabs 1kb Plus DNA Ladder for Safe Stains (product #N0559S)<\/p>\n
\n
Our second PCR reaction was set up on Oct. 28, 2025, used the same Taq polymerase as the first PCR reaction, and:<\/li>\n
was a dublex reaction that used the arthropod F and R and Wolbachia R and F primers.<\/li>\n
used an annealing temperature of 49 degrees Celsius<\/li>\n
differed from the standard protocol (differences in amount of DNA per sample, accidental mistakes, etc.) in that: – used 1.5 UL of (+) DNA control with .5 UL<\/li>\n<\/ul>\n",No,TTTGAGCTGGTATAATCGGTTCATCTATAAGAATAATTATTCGTCTAGAATTAGGATCATCTAATTCATTGATTAACAATGATCAAATTTATAATTCTATAGTTACAAGACATGCATTCATTATAATTTTCTTTATAGTTATGCCTTTCATAATTGGAGGATTTGGAAATTTCCTTGTACCTTTAATATTAGGCTCACCTGATATAGCTTATCCACGTATAAACAATATGAGATTTTGACTTTTACCCCCCTCAATTTCTCTTCTTATTTTAAGAAATTTTATTAATGATGGTGTTGGGACAGGATGAACTGTTTATCCTCCTTTAGCTTCAAATATTTTCCATAATGGACCTTCAGTTGATTTAACTATTTTTTCCCTCCATATTGCGGGTATATCTTCAATTTTAGGAGCTATTAATTTTATTTCAACTATCTTAAATATACATCACAAAAATTTTTCTATCGATAAAATCCCTTTACTTGTATGATCAATTTTAATTACTGCAATTTTACTTCTCTTATCCTTACCCGTTCTTGCAGGAGCTATTACTATACTTTTAACTGACCGTAATCTTAACACTTCATTTTTTGATCCATCAGGTGGTGGAGATCCTATTTTATATCAACATCTTTTTTGA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/tempImagehk9nxk-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/tempImagefhFlLm-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/tempImageihcBTe-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/tempImageYMKk5E-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/tempImageIw34wf-scaled.jpg,",42.3729519000,-73.3676804000,09/28/2025,"
Was found flying near the hive that is within the walls of a house but was collected while it was outdoors. Was not entering hive but flying and landing above the hive on the wall $\"\"$ <\/a>. Moving in an active and energetic way.<\/p>\n",Vespula germanica,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_7871-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_3120.jpeg,","
We used DNA extraction protocol based on the insect adaptation of New England Biolabs’ Monarch Spin gDNA Extraction Kit (Product #T3010).<\/p>\n
The specimen was incubated for 33 minutes in a hot water bath at 56 degrees C.<\/p>\n
Details that differed from the written protocol are I forgot to dump the buffer solution at the bottom of the tube before I put the second wash buffer but I dumped it all out after pouring the second wash buffer in and before centrifuging.<\/p>\n
Our first PCR reaction was set up on October 14 2025 and:<\/p>\n
\n
was a duplex reaction because we used both the Arthropod CO1 and Wolbachia 16S primers together<\/li>\n
used this Taq polymerase: New England Biolabs Onetaq Hot Start Quick-Load 2x Master Mix with Standard Buffer (#M0488S)<\/li>\n<\/ol>\n
Our first gel image was taken on 10\/21\/25 and:<\/p>\n
\n
was run at 125 volts for 20 minutes<\/li>\n
used this DNA ladder: New England Biolabs 1kb Plus DNA Ladder for Safe Stains (product #N0559S)<\/li>\n<\/ul>\n
Our second PCR reaction was set up on October 28th, used the same Taq polyamerase as the first PCR reaction, and:<\/p>\n
\n
was a duplex reaction that used arthropod F and R and Wolbachia R and F primers.<\/li>\n
\u00a0used an annealing temperature of 49 degress Celsius.<\/li>\n
differed from the standard protocol in that: I used 1.5 uL of positive DNA control with .5 uL of water since we had a smear last time.<\/li>\n<\/ul>\n
Our second gel image was taken on 11\/13\/25 and:<\/p>\n
\n
was run at 125 volts for 21 mins<\/li>\n
used this DNA ladder: New England Biolabs 1kb Plus DNA ladder for Safe Stains<\/li>\n<\/ul>\n",No,TGAGCAGGAACTTTAGGAGCTTCAATAAGAATAATTATTCGTTTAGAATTAAGATCCCCTGGAGCTTTAATTAATAATGATCAAATTTATAATACTATTATTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTTATACCTTTTTTAGTTGGAGGATTTGGAAATTGATTAATCCCTTTAATATTAGGTGTTCCTGATATAGCATTTCCTCGAATAAATAATATAAGATTTTGATTATTACCTCCATCCTTATTTTTATTAATCTTAAGAAATTTTATTGGAACCGGAGTAGGGACAGGATGAACTCTGTACCCTCCTTTATCATCAATTGTAGGACATGACTCTCCCTCTGTAGACTTAGGAATTTTTTCTATCCATATTGCTGGAATTTCATCAATTATAGGTTCAATTAATTTTATTGTTACTATTTTAAATATACACACAAAAACACATTCACTAAATTTTCTTCCTTTATTTTCATGATCAATTTTAATTACAGCAATTCTTCTCTTGTTATCTCTACCAGTTCTTGCAGGAGCAATTACTATACTTCTTACAGACCGTAATTTAAATACATCTTTCTTTGATCCTGCAGGCGGAGGTGACCCAATTTTATACCAACATTTA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_7951-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_7952-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_7955-scaled.jpeg,",42.4500845000,-73.2453824000,09/28/2025,"
When collected, arthropod was hanging off a branch of a red oak tree in my backyard (a more rural area). When first collected, color was more gray. After putting in alcohol to preserve, turn more of a dark red color.<\/p>\n",Maevia inclemens,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_8241-2.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_8416-1.jpeg,","
We used a DNA extraction protocol based on the insect adaptation of New England biolabs’ monarch spin gDNA Extraction kit (product #T3010)<\/p>\n
The specimen was incubated for 30 minutes in a hot water bath at 56 degrees C<\/p>\n
Our first PCR reaction was set up on 10\/14\/25. It was a duplex reaction because we used both the Arthropod CO1 and Wolbachia 16S primers together. The annealing temperature was 49 degrees C. We used this Taq polymerase: New England Biolabs OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer (#M0488S). The only difference from written protocol was that we used 88 ul of Taq Master Mix instead of 87.5.<\/p>\n
Our first Gel image was taken on 10\/21\/25 and was run at 125 volts for 22 minutes. The DNA Ladder used was New England Biolabs 1 kb Plus DNA Ladder for safe stains (product #N0559S)<\/p>\n
Our second PCR reaction was set up on 10\/28\/25, used the same taq polymerase as the first PCR reaction and: was a single PCR reaction that used the wolbachia 16s primers, used an annealing temperature of 55 degrees Celsius, the only thing we did differently from the standard protocol is that we used 88 ul of taq polymerase.<\/p>\n
Our second gel image was taken on 11-4-25 and was run at 125 volts for 27 minutes. The DNA Ladder used was New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product #N0559S)<\/p>\n",Unknown,>1-HannahB-spider-duplex-CO1F GATGATCAAATTTATAATGTAATTGTAACTGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGTGGATTTGGAAATTGATTAGTGCCTTTAATATTAGGTGCTCCTGATATAGCATTCCCACGAATAAATAATATAAGATTTTGACTTCTACCTCCTGCTCTTTCTTTACTATTAGTAAGTAGAATAGTTGAAAATGGAGCTGGGACAGGATGAACTGTTTATCCACCTCTATCCGCTGGAATTGCTCATGGTGGAGCTTCAGTTGATTTAGCTATTTTTTCTCTACATTTAGCAGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACAACTGTAATTAATATACGATCAACAGGAATTTCATTAGATCGTATACCTTTATTTGTTTGATCAGTAGTTATTACTGCTTTATTATTATTATTATCACTTCCAGTACTAGCAGGAGCTATTACTATATTATTAACAGATCGAAATTTAAATACATCATTTTTTGACCCAGCGGGAGGAGGAGATCCTATTTTATACCAACATTTATTTTGA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_2588-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_2586-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_2585-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_2587-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_2589-scaled.jpeg,",42.4623740000,-73.2471081000,09/29/2025,"
Arthropod was collected outdoors in a dirt area.<\/p>\n",Lasius alienus,Monarch DNA extraction (NEB),Whole arthropod,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_2734.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_2785-1.jpeg,","
We used a dna extraction protocol based on the insect adaptation of New England biolabs monarch spin gDNA extraction kit (product #T3010)<\/p>\n
the specimen was incubated for 30 minutes in a hot water bath at 56 degrees C.<\/p>\n
Our first PCR was set up on 10\/14\/25 and was a duplex reaction because we used both the arthropod CO1 and Wolbachia 16S Primers together. Used an annealing temperature of 49 degrees Celsius. Used this TAQ polymerase: New England Biolabs OneTaq Hot start Quick Load 2X Master Mix with standard buffer \u00a0(#M0488S).
\nDiffered from the written protocol: used 88 ul of Taq master mix<\/p>\n
Our first gel image was taken on 10\/21\/25 and was run at 125 volts for 22 minutes. Used this DNA Ladder : New England Biolabs 1 kb Plus DNA Ladder for safe stains (product #N0559S)<\/p>\n
Our second PCR reaction was set on 10\/28\/25 used the same Taq polymerase as the first PCR reaction, and was a single reaction that used Wolbachia F and R primers. Used an annealing temperature of 55 degrees Celsius.
\nDiffered from the standard protocol in that : used 88uL instead of 87.5<\/p>\n
Our second gel image was taken on 11\/4\/25 and was run at 125 volts for 27 minutes.
\nused this DNA ladder: New England BioLabs 1kb plus DNA Ladder for Safe Stains (product # N0559S)<\/p>\n",No,AAATTTATAACTCTATAGTTACAAGGCATGCATTTGTTATAATTTTCTTCATAGTTATACCTTTCATAATTGGTGGATTTGGTAATTTTCTTGTACCTTTAATATTAGGTTCACCTGATATGGCTTACCCCCGTATAAATAATATAAGATTTTGACTTTTACCCCCCTCTATTTCTCTTCTCCTTTTAAGAAATTTCATTAATGATGGAGTCGGAACAGGATGAACCATTTATCCTCCTTTAGCCTCAAACATCTTCCATAATGGCCCTTCAGTTGATTTAACTATTTTCTCTCTTCACATCGCTGGAATATCTTCTATTCTAGGGGCTATTAATTTTATTTCAACTATCATAAACATACACCATAAAAATTTTTCTATTGATAAAATTCCACTACTTGTATGATCAATCTTAATTACTGCAATTTTACTACTTCTATCCCTTCCTGTTCTTGCAGGAGCTATTACTATACTTTTAACTGACCGTAACCTTAATACTTCATTTTTTGACCCATCAGGAGGTGGTGATCCTATTTTATATCAACATCTTTTCTGA,,, "",44.9262274000,-93.1742515000,09/17/2025,"
Found on a wall by other ants. Black. Bigger than a grain of rice.<\/p>\n",Formica,Qiagen DNeasy Blood and Tissue Kit,Abdomen,MiniPCR,TBE,SeeGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-10-154712.png,","
Methods:<\/p>\n
\n
sample prep<\/li>\n
cell lysis + DNA precipitation<\/li>\n
DNA purification<\/li>\n
DNA elution<\/li>\n<\/ol>\n",Yes,,,, "",44.9264611487,-93.1725916266,09/17/2025,"
Ant was black and was smaller than a grain of rice. It was found between cracks in a sidewalk.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SeeGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Screenshot-2025-12-09-101145-1.png,","
My arthropod’s DNA was in lanes 6 and 7. Lane 6 contained AK1A and tested for arthropod DNA, and lane 7 contained AK1W and tested for Wolbachia DNA.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/1-1.jpg,",38.92801223520854,-77.0721110773523,09/04/2025,"",Atalopedes campestris,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Screenshot-2025-10-27-at-9.53.11 AM-1.png,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Finn_moth-CO1F-2.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/5-1.jpg,",38.9288746221,-77.0676958774,09/04/2025,"
Caught in a wooded, leafy area, flying low to the ground. Irratic movement, but calmed down a bit. Other bees flying around near it.<\/p>\n",Vespula flavopilosa,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Desktop-screenshot-10-23-2025_08_32_AM.png,","",No,AATTATTCGTTTAGAATTAAGATCTCCTGGAGCTTTAATTAATAATGATCAAATTTATAATACAATTATTACAGCCCATG CCTTTATTATAATTTTTTTTATAGTAATACCTTTTTTAGTTGGAGGATTCGGAAATTGATTAATCCCCTTAATATTAGGA GTGCCTGATATAGCATTTCCTCGAATAAATAATATAAGATTTTGATTACTTCCCCCTTCATTATTTTTATTAATTTTAAG AAATTTTATTGGAACCGGAGTAGGAACGGGATGAACTTTATACCCTCCCTTATCATCTATTGTTGGTCATGATTCACCTT CTGTAGATTTAGGAATTTTTTCTATTCATATTGCTGGAATTTCCTCAATTATAGGATCAATTAATTTTATTGTCACTATT TTAAATATAC,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Rafi_bee-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/DSC10301-scaled.jpg,",43.45335697415103,-111.9577952395874,06/09/2025,"
Caught live crawling near a ninebark.<\/p>\n",Trochosa ruricola,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/PXL_20250612_1930326602-scaled.jpg,","
Results are fifth row. Top: CO1. Bottom: Wol.<\/p>\n",No,GATCTNGCTATAGTGGGGACTGCTATAAGAGTGTTAATTCGTTTAGAATTAGGAAATTCTGGAAGTTTATTAGGAGATGATCATTTATATAATGTTATAGTTACTGCTCATGCTTTTGTGATGATTTTTTTTATAGTAATGCCAATTCTTATTGGAGGTTTTGGTAATTGATTAATTCCTTTAATGTTAGGTGCTCCTGATATATCATTTCCTCGAATGAATAATCTTTCTTTTTGGTTATTGCCCCCTTCTTTATTTTTATTAACTATGTCTTCTATAGTGGAAATAGGGGTAGGAGCTGGTTGAACAGTTTATCCTCCTTTAGCATCTAGAATAGGTCATATAGGGAGTTCTATAGATTTTGCTATTTTTTCTCTTCATTTAGCTGGTGCTTCTTCTATTATAGGGGCAGTAAATTTTATTTCTACTATTATTAATATACGAATATTAGGTATATCTATAGATAAGGTACCTTTATTTGTTTGGTCAGTTTTGATTACGGCTGTTTTATTATTATTATCTTTACCTGTTTTAGCAGGTGCTATTACTATGTTATTAACTGATCGAAATTTTAATACTTCTTTTTTTGACCCTGCAGGGGGAGGAGATCCTATTTTATTTCAACATTTGTTTTGATTTTTTG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/DSC10300-scaled.jpg,",43.45337286370766,-111.95778580299253,06/09/2025,"
Caught live near yard.<\/p>\n",Anthrenus,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/PXL_20250612_1930326602-scaled.jpg,","
Results are fourth row. Top: CO1. Bottom: Wol.<\/p>\n",Yes,GGAACTTCNTTANGAGTATTAGTCCGGACAGAATTAGGTAATCCAGGATCCCTTATTGGAGATGACCAAACATTCAGTGTAATCGTAACAGCCCATGCATTCATTATAATTTTTTTTATAGTTATACCAATTATAATTGGTGGATTCGGTAACTGATTAGTACCCTTAATATTAGGTGCTCCAGATATAGCATTTCCACGAATAAATAATATAAGATTCTGACTTCTACCCCCATCACTATCATTGTTATTAATAAGAAGAATAGTAGAAAAAGGGGCAGGAACAGGATGGACAGTTTACCCCCCACTATCAAGAAATATCGCCCACGGAGGAGCATCAGTAGACCTAGCAATCTTTAGATTACATCTAGCAGGAATCTCATCAATTCTAGGAGCTATTAACTTCATCACCACAGCTATCAATATACGAACTATAGGAATAACTCCAGAACGAATACCCCTATTTGTCTGATCAGTAGCAATTACTGCCCTTCTCTTACTACTCTCATTACCAGTACTAGCAGGAGCTATTACAATACTATTAACAGACCGAAATCTCAATACATCATTCTTTGATCCATCAGGTGGGGGAGACCCAATCCTATACCAACATCTATTCTGATTTTTTGGTCAC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/DSC10299-scaled.jpg,",43.45335697415103,-111.95777378191528,06/09/2025,"
Collected dead from a fertilizer spreader.<\/p>\n",Apis mellifera,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/PXL_20250612_1930326602-scaled.jpg,","
Results are third row. Top: CO1. Bottom: Wol.<\/p>\n",No,TTTATGATCTGNANNCTAGGATCATCAATGAGACTTATTATTCGAATAGAATTAAGATCCCCAGGATCATGAATTAGCAATGATCAAATTTATAATACAATTGTTACTAGTCATGCATTCCTAATAATTTTTTTTATAGTTATACCATTTTTAATTGGAGGATTTGGAAATTGGCTTATTCCTTTAATACTAGGATCACCTGATATAGCATTCCCCCGAATAAATAATATTAGATTTTGATTACTTCCTCCCTCATTATTTATACTTTTATTAAGAAATTTATTTTATCCAAGACCAGGAACTGGATGAACAGTATATCCACCATTATCAGCATATTTATATCATTCTTCACCTTCAGTAGATTTTGCAATTTTTTCTCTTCATATATCAGGAATTTCCTCAATTATAGGATCATTAAACTTAATAGTTACAATTATAATAATAAAAAATTTTTCTATAAATTATGACCAAATTTCATTATTTCCATGATCAGTTTTTATTACAGCAATTTTATTAATTATATCATTACCTGTATTAGCTGGAGCAATTACTATACTATTATTTGATCGAAATTTTAATACATCATTTTTCGATCCTATAGGAGGTGGAGATCCAATTCTTTATCAACATTTATTTTGATTTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/DSC10296-scaled.jpg,",43.45337001611267,-111.95778800266623,06/09/2025,"
Caught live crawling in ground cover.<\/p>\n",Armadillidium vulgare,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/PXL_20250612_1930326602-scaled.jpg,","
Results are the second row. Top: CO1. Bottom: Wol.<\/p>\n",No,GGGGTTTGAGCAGGNGCTGTTGGAACTGCCCTTAGAATAATTATTCGCACTGAATTAGGACAACCTGGAAGTTTAATTGGAGATGACCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATACCTATTATAATTGGTGGATTTGGTAATTGGTTAATTCCATTAATACTAGGAGCCCCAGATATGGCTTTTCCACGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCTTTAACTTTATTGCTTAGAAGTGGGTTGGTTGAAAGAGGAGTAGGAACAGGATGGACAGTATATCCTCCGCTGGCGTCTAGAATTGCTCATAGAGGAGCATCTGTAGATTTAGGTATTTTTTCTCTCCACCTTGCTGGAGCTTCTTCTATTTTAGGGGCTGTGAATTTTATTACTACTATAATTAATATACGAGCAGCTGGAATTAGAATAGACCGTGTTCCTTTATTTGTTTGATCAGTATTAGTAACGGCTGTACTTTTACTTTTATCCCTACCTGTATTAGCAGGAGCTATTACTATATTATTAACTGATCGAAATTTAAATACCTCTTTTTTTGACCCTAGGGGAGGAGGAGACCCTATCCTTTATCAACACTTATTTTGATTTTTTG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3859-1-scaled.jpeg,",42.3713602112,-73.3671808148,10/01/2025,"
Found in Richmond Massachusetts alive outside a large barn on the side of a lightly forested field sample harvested 10\/1\/25<\/p>\n",Polistes dorsalis,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/att.BbAxJ_lCBj2Khub4YeiTmeJE3Gph4X3ypfAPVZPCe8o.jpeg,","
Are duplex PCR ran on October 16th that used the primers for Wolbachia 16s forward and reverse and the primers for arthropod _f (LCO1490) and Arthropod_R (HCO2198) They were ran at an annealing temperature of 49 degrees Celsius this was conducted twice once for are first round and second round to be u used in the gel electrophoresis trial<\/p>\n
Are first gel image was taken on october 23 10\/23\/25 and was run on 125 volts for 20 minutes the DNA ladder used : new England Biolabs 1 kb plus DNA ladder for safe stains (product#N0559S)<\/p>\n
are second gel image taken on 11\/6\/25 using the Edvotek gel Electrophoresis was ran at 125 volts for 25 minutes the DNA ladder used was the new England bio labs 1 KB plus DNA ladder for safe stains (product # N0559S)<\/p>\n",No,TGATCAGGAATAATTGGAAGATCTTTAAGATTAATTATTCGTTTAGAATTAGGAACACCTTTATCAATTATTAATAATGATCAAATTTATAACTCATTTATTACAGCACATGCATTAATTATAATTTTTTTTATAGTTATACCATTTATAATTGGAGGATTTGGAAATTGATTAATTCCTCTAATATTAGGTGCTCCAGATATAGCATTTCCACGAATAAATAACATAAGATTTTGATTATTACCTCCATCCCTATTACTACTAATTATTAGAAATATAAATGATTCAGGAGTAGGAACTGGATGAACTTTATATCCACCTTTATCATCTAATATAGGTCATAATTCATCATCAGTTGATTTTGCTATTTTTTCACTTCATATCGCAGGAATTTCATCAATTATAGGAGCTATTAATTTTATTGTAACAATTTTAAATATACATATTAAAACTTATTCATTAAATTTTTTACCAATATTTACATGATCAGTTTTAATTACTGCAATTTTACTTTTATTATCTTTACCTGTACTTGCTGGAGCAATTACAATATTATTAACTGATCGAAATATTAATACATCTTTCTTTGATCCATTAGGAGGTGGAGATCCAATTCTATTTCAACATTTATTTTGA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/DSC10283-scaled.jpg,",43.4926835501,-111.9844242107,06/04/2025,"
Collected live from ground cover mulch.<\/p>\n",Phalangium opilio,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/June10thElectrophoresis-scaled.jpg,","
Results are fifth row. Top: CO1. Bottom: Wol.<\/p>\n",No,GGGCTGCTATAATTGGGACAGCCCTTAGTATACTAATCCGAGCTGAATTGGGCCAACCTGGGTCAATAATAAATGATGATCAAATTTATAATGTTATTGTAACCGCCCATGCCTTTGTTATGATTTTCTTTATAGTAATACCTATTATAATTGGAGGGTTTGGAAACTGATTAGTCCCATTAATATTAGGAGCCCCTGATATAGCCTTCCCCCGACTTAATAATATAAGATTTTGATTGCTCCCCCCTTCTTTTTTACTATTACTATCATCAACAATGGTAGAAAACGGAGCAGGAACAGGATGAACAGTATACCCCCCTTTATCAAGAAATATTGCTCATACAGGGGCCTCTGTAGATTTAACTATTTTTTCATTACACTTAGCAGGTGTATCCTCAATTTTAGGGGCTATCAATTTTATTACTACAATTATCAATATACGAACACAAGGAATAATTATAGAACGAATACCTCTTTTTGTCTGATCAGTAAAAATTACAGCTATTTTACTTCTTCTATCTTTGCCTGTTTTAGCAGGTGCTATCACAATACTTCTTACAGATCGAAATTTTAACACATCCTTCTTCGACCCCGCAGGAGGGGGAGACCCTATTCTTTACCAGCATTTATTTTGATTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/DSC10282-scaled.jpg,",43.4927047211,-111.9844108080,06/04/2025,"
Collected live from the sidewalk.<\/p>\n",Tetramorium immigrans,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/June10thElectrophoresis-scaled.jpg,","
Results are the fourth row. Top: CO1. Bottom: Wol.<\/p>\n",No,AANCGGTCTTCATAAGTATAATTATCCGACTTGAANTAAGNTCCTGTGGTTCATTAATTAATGACGACCAAGTNTATAATTCCTTAGTAACAAGACATGCCCTTGTTATAATTTTCTTTATAGTAATGCCTTTTATAATTNGNGGNNNNNNNNNTTTTAGTTCCATTTAATACTAGGAATCTCCCGATANTAGCCTACCCCCGAATAAATAACATAAGATTTTGACTTCTNCCCCCCNCTATAAGATTATTATTGCTTAGAAAGATTTATTAATCCGGGGGAGGGAACAGGAAGAACTATCNNTCCCCCNTNAGCATCTATTATNTTTCNANNAGGCCCNTCAATTGATCTATCAATTTTTTCTCTTCAAATGGCAGGNNNNNNTTNTANTTANNGGGGCAATTANTTTTATNGCTACNANTATAAACATACNTCATAAAAAATTAACCTTANATAAAANCCCCCGGTTAGTTTGANNNNTTTAATTACAGCAATNCTNNNCCNCCTATCTCTNCCGNATNAGNGGGAGCTATTACTNTACTCTNACAGANCGAATTNAANACNTCATTTTTTGANCCATCGGGGGGGGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/DSC10281-scaled.jpg,",43.4926807032,-111.9844147899,06/04/2025,"
Collected live off of a crabapple tree.<\/p>\n",Cerceris nigrescens,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/June10thElectrophoresis-scaled.jpg,","
Results are third row. Top: CO1. Bottom: Wol.<\/p>\n",No,TTAGNGAGCATCNATTCNAGTATAATAATTCGTTTAGAACTTAGATCTCCAGGTAGATTTATTAATAACAGACAATTATATAATAGAATTGTAACTACACATGCATTTATTATAATTTTTTTTATAGTTATACCATTCATAATTGGTGGATTTGGAAACTGATTAATTCCTATAATATTAGGAGCTCCAGACATAGCATTTCCACGAATAAATAATATAAGATTTTGACTTTTACCACCTTCATTAATTTTTTTATTATTAAGANCAATTACAAATCAAGGATTAGGAACAGGANGAACTTTATACCCACCACTTTCTAGAAATACTGGACACAGAAGANTNNCTGNTGATATATCTATTTTTTCATTACATATAGCAGGAANTTCNTCTANTATAGGNGCTANTAATTTTANTGNAACAATTATTAATANACATAATAAAAANANTGNCATAGAANNNTACAATTACNTAATTGNCNNTTTAACAACTGCNANTCTTTTACTCTTATCTTACCAGACTTGCTGGNNANCACTATACTTTATTGANCGAATTAATACNTCATTTTTGANCCATCAGGAGGGGGGACCNNNNTTNNCACTTAT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/DSC10280-scaled.jpg,",43.4926887737,-111.9844223539,06/04/2025,"
Collected live off of a raspberry bush.<\/p>\n",Agrilus cuprescens,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/June10thElectrophoresis-scaled.jpg,","
Test results are the second row. Top: CO1. Bottom: Wol.<\/p>\n",Yes,GGGGCCTGATCAGGNATAGTGGGTACCGCTCTTAGATTATTAATCCGAGCAGAACTCGGAAACCCCGGAGCTTTAATTGGAAATGATCAGATTTATAATGTTATCGTAACTGCTCACGCTTTTATTATAATCTTCTTTATGGTAATACCTATCATAATAGGGGGTTTCGGAAATTGACTTGTTCCTCTAATGCTAGGCGCTCCTGACATAGCCTTCCCTCGGATAAACAATATAAGATTCTGACTACTCCCCCCTTCTTTAACCTTACTTTTAATAAGTAGAATAGTAGAAAGAGGAGCAGGAACAGGTTGAACTGTTTACCCTCCTTTAGCAGCAAATATTGCTCACAGAGGAGGTTCTGTAGATTTGGCAATCTTTAGTTTACATTTAGCAGGGATCTCCTCTATTTTAGGTGCCATTAATTTTATTACAACAGTAATTAATATACGAGCTGTAGGAATAACAATAGACCGGGTACCTTTATTAGTTTGGTCAATTGCTATCACTGCACTCCTTCTCTTGTTATCTTTACCAGTACTAGCAGGAGCCATTACTATACTACTCACTGACCGAAATTTAAATACATCCTTCTTTGACCCGGCAGGGGGAGGAGACCCTATTCTTTATCAGCATTTATTCTGATTTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/6.jpg,",39.0257081303,-77.1575846983,09/02/2025,"
Found under the eaves of a porch. Damaged horn, couldn’t walk, seemed like it was dying.<\/p>\n",Dynastes tityus,DNeasy (Qiagen),Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Screenshot-2025-10-20-130339.png,","",No,TCACTAAGTCTCCTAATTCGAGCCGAACTAGGAAACCCTGGGTCATTAATTGGTGATGATCAAATCTATAATGTTATCGTAACAGCCCACGCTTTTATTATAATTTTTTTTATAGTTATACCCATTATAATTGGGGGATTCGGAAATTGGCTTGTCCCCCTAATACTTGGGGCCCCGGATATAGCCTTCCCACGAATAAATAATATAAGATTCTGGCTCCTCCCACCCTCATTAACCCTTCTTCTAGCCAGAAGAATAGTAGAAAACGGGGCCGGAACTGGCTGAACAGTATACCCTCCCCTCTCTGGGAACATTGCTCNTAGAGGAGCCTCTGTTGATCTCGCAATTTTTAGGCTACACTTAGCAGGANTTTCTTCTATTCTAGGTGCAGTAAATTTTATTACGACTGTAATTAATATNCGTTCAACCGGAATATCTTTTGACCG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/4-scaled.jpg,",38.9293021080,-77.0695827681,09/04/2025,"
I collected this carpenter ant in the woods on STA’s campus. I collected it in the late morning in late summer\/early fall and the temperature was around 70 degrees F outside.<\/p>\n",Hymenoptera,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Screenshot-2025-10-17-at-8.13.48 AM.png,","
I crushed the carpenter ant up really well and cut off its legs and head so I could isolate the abdomen. I got a band for CO1, meaning that I was able to extract arthropod DNA from my ant.<\/p>\n",No,nnnTTTGATCnGGNnTAATTGGTTCTTCnnnnAGAATAATTATTCGATTAGAATTAGGATCCCCTGATTCACTAATTCTTAATGATCAAACCTTCAATACTATCGTTACAAGCCATGCTTTTATTATAATCTTTTTCATAGTTATACCTTTTATAATTGGAGGnTTTGGTAACTTCTTAGTACCCTTAATACTTGGGTCTCCTGATATAGCCTATCCACGCATAAATAACATAAGATTCTGATTACTACCCCCTTCAATTTCTCTATTAATCTTAAGAAATTTTATTAATGAAGGATCAGGAACAGGTTGAACTATTTATCCCCCCCTATCATCTAATACCTTTCATAGTGGTCCCTCTATTGATTTAACTATTTTTTCCCTTCATATTGCCGGAATATCATCAATTCTAGGGGCAATCAATTTTATTTCAACAATTTTAAACATACATAAnTCTAATATTACCTTAGATAAAAnTCCCCTATTAGTAnGATCTATTCTTATCACTGCTATTCTCCTCCTCCTCTCCCTTCCTATTCTTnnAGGAnCTATTACAATACTACTTACnGAnCGAAAnCTCnnTACTTCAnTTTTTnnnCCCTCAGGAnGAGGAnAnCCTnnTCTATnnnnnCACTTnnTTT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Eamonn_ant-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/7a.jpg,",38.92809145363613,-77.07160709536669,,"",Insecta,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Screenshot-2025-10-17-at-8.37.09 AM.png,","
]<\/p>\n",No,AGCACTAAGAACATTAATTCGAATTGAATTGGGAATTCCTGGGTCATTCATTGGTGATGATCAAATTTATAATGTTGTTG TTACAGCTCATGCATTTATTATAATTTTTTTCATGGTTATACCTATTATAATAGGAGGATTTGGTAATTGACTTGTACCA TTGATAATTGGGGCACCTGATATGGCTTTTCCTCGAATAAATAATATAAGATTTTGACTTTTGCCCCCTTCTTTGACCTT ATTATTAGTTGGAAGATTAGTTGATAGAGGTGCTGGAACTGGTTGAACAGTTTATCCTCCTTTATCAAGATACATATTTC ATTCTGGTTCTTGTGTTGATTTAACAATTTTTTCTTTACATTTGGCAGGTGTATCTTCAATTTTGGGGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/20250926_150455-scaled.jpg,",38.9866412000,-77.0615970000,09/20/2025,"
\n
Brownish color<\/li>\n
Terrestrial area w\/ lots of leaves & dirt<\/li>\n
Caught summer going into fall<\/li>\n
73 degrees when caught<\/li>\n
Very slim<\/li>\n
8+ legs<\/li>\n
Antennaes<\/li>\n<\/ul>\n",Philoscia muscorum,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Image-10-16-25-at-10.02 AM.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-04-at-1.36.31 PM-1.png,","
DNA Extraction<\/p>\n
\n
The arthropod crushed up easily<\/li>\n<\/ul>\n
PCR<\/p>\n
\n
Everything went as planned<\/li>\n<\/ul>\n
Gel Electrophoresis, Arthropod CO1<\/p>\n
Image 1:<\/p>\n
\n
Lane 1 = DNA LADDER<\/li>\n
Lane 2 = A-1<\/li>\n
Lane 3 = A-2<\/li>\n
Lane 4 = A-3<\/li>\n
Lane 5 = A-4<\/li>\n
Lane 6 = Wolbachia (+)<\/li>\n
Lane 7 = Wolbachia (-)<\/li>\n
Lane 8 = H20<\/li>\n<\/ul>\n
Image 2:<\/p>\n
\n
Lane 1 = DNA LADDER<\/li>\n
Lane 2 = A-5<\/li>\n
Lane 3 = A-6<\/li>\n
Lane 4 = Wolbachia (+)<\/li>\n
Lane 5 = Wolbachia (-)<\/li>\n
Lane 6 = H20<\/li>\n<\/ul>\n
Gel Electrophoresis, Wolbachia<\/p>\n
\n
Everything went as planned, our controls worked<\/li>\n<\/ul>\n
<\/p>\n
\n
Lane 1 = DNA LADDER<\/li>\n
Lane 2 = A-1<\/li>\n
Lane 3 = A-2<\/li>\n
Lane 4 = A-3<\/li>\n
Lane 5 = A-4<\/li>\n
Lane 6 = Wolbachia (+)<\/li>\n
Lane 7 = Wolbachia (-)<\/li>\n
Lane 8 = H20<\/li>\n<\/ul>\n
<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/A4CO1-CO1F_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/spider-and-ruler-rotated.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/spider-face-rotated.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/spider-fangs-2-rotated.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/spider-fangs-rotated.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/spider-under-belly-rotated.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/spider-underberlly-2-rotated.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/top-of-spider-rotated.jpeg,",42.4634503000,-73.3001233000,10/02/2025,"
I collected the spider, from outside a highly populated apartment complex in downtown Pittsfield. The spider had made its web on my grill in the front yard. I collected it alive, brought it to the lab, and then placed it in a tube with 70% ethanol to preserve cellular\/molecular and DNA data.<\/p>\n",Larinioides sclopetarius,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/GEL-ELECTRO-1-DUPLEX-PCR.jpg,","
Week of 10\/7\/25 – We used a DNA extraction protocol based on the insect adaptation of New England Biolabs’ Monarch Spin gDNA extraction kit (Product # T3010), using the spider’s abdomen. The specimen was then incubated for 30 minutes in a hot water bath at 56 degrees C.<\/p>\n
Week of 10\/14\/25 – Our first PCR reaction was set up on 10\/16 and was a duplex reaction because we used both the Arthropod CO1 and Wolbachia 16S primers together. We used an annealing temperature of 49 degrees Celsius. We used Taq polymerase: New England Biolabs One Taq Hot Start Quick-Load 2x Master Mix with Standard Buffer (#M0488S).<\/p>\n
Week of 10\/21\/25 – Our first gel image was taken on 10\/23\/25 and was run at 125 volts with 0.2 current, for 20-30 minutes. We used DNA Ladder: New England Biolabs 1kb Plus DNA Ladder for Safe Stains (product # N0559S).<\/p>\n",No,GTGCTTGGGCTGCTATAGTAGGAACTGCAATGAGAGTGTTAATTCGAATTGAATTAGGTCAACCTGGTAGATTTATAGGGGATGATCAATTATATAATGTTATTGTTACTGCTCATGCTTTTGTGATAATTTTTTTTATAGTAATACCTATTTTAATTGGGGGATTTGGAAATTGATTGGTTCCTTTAATATTAGGAGCACCTGATATGGCTTTTCCTCGAATAAATAATTTAAGATTTTGATTACTCCCCCCGTCATTATTTTTATTGATTGTTTCATCAATGGTGGAAATAGGAGTAGGTGCTGGGTGAACTGTTTATCCTCCTTTAGCAAGGTTAGAAGGTCATGCCGGGAGGTCAGTTGATTTTGCTATTTTTTCATTACATTTAGCTGGGGCGTCATCAATTATAGGTGCTATTAATTTTATTTCAACAATTATTAATATACGTTTTTATGGAATAACCATGGAAAAAGTTCCATTATTTGTATGATCAGTACTAATTACTGCTGTATTATTACTTCTTTCGTTACCAGTATTAGCTGGGGCTATTACAATATTATTAACAAATCGAAATTTTAATACATCTTTTTTTGACCCTTCTGGTGGGGGAGACCCAATTTTGTTTCAACACTTA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_1450-1.jpeg,",40.5472763000,-75.4169918000,,"",,DNeasy (Qiagen),Abdomen,Standard electrophoresis system Bio-Rad,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_1450-2.jpeg,","
1-8,<\/p>\n
Common hentz jumping spider (first line)<\/p>\n
Praying mantis (second line)<\/p>\n
Wolbachia present in spider.<\/p>\n
9-16:<\/p>\n
first line: European harvestman spider<\/p>\n
second line: Tussock moth caterpillar<\/p>\n
No Wolbachia present<\/p>\n",Yes,NNNNNNNNNNNNGNTGCTGAGCAGGAATATTAGGGACATCATTAAGAATTCTAATTCGAACTGAATTAGGACAACCCGGT TCCCTTATTGGTGATGATCAAATTTATAATGTTATCGTAACCGCTCATGCTTTCATTATAATTTTCTTCATAGTAATACC AATTATAATTGGTGGATTTGGAAATTGACTTGTACCACTTATATTAGGAGCCCCAGATATAGCTTTTCCCCGAATAAATA ACATAAGATTTTGACTTTTACCACCATCAATTTTATTATTATTAATTAGAAGAATAGTAGAAAGAGGTGCAGGAACAGGA TGAACTGTTTACCCCCCTCTTTCAGCTAGAATTGCTCATGCAGGACCCGCAGTAGATTTAACAATTTTCTCCTTACATCT TGCAGGTATATCAAGAATTATAGGAGCAGTAAATTTTATTACAACCATAATTAATATAAAACCCCTTTACATAAACCAAA CACAAGTACCCCTTTTCGTATGATCTGTAGGAATTACCGCTCTACTTTTATTATTATCACTTCCTGTACTTGCAGGAGCA ATTACTATACTTTTAACTGATCGAAATCTTAATACTTCATTTTTTGACCCAGCAGGGGGAGGAGATCCAATTTTATATCA ACACCTATTTTGATTTTTNGNNN,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3138-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3135-scaled.jpeg,",38.9300000000,-77.1600000000,09/07/2025,"
Brown with black spots and red spot beneath wings. 2 wings. 6 legs. Oval abdomen. No visible antennae.<\/p>\n
Captured on a concrete pool deck.<\/p>\n
Late summer<\/p>\n
Temperature: 75\u00ba<\/p>\n
Habitat: urban<\/p>\n",Hemiptera,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_2984.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_2962.jpeg,","
We added 10 extra microliters or Proteinase K during DNA extraction. We accidentally filled two wells with DNA in the CO1 gel, but no contamination.<\/p>\n
Gel electrophoresis, Arthropod CO1: DNA ladder in lane 1, C-5 in lane 2, C-6 in lane 3, C-6 in lane 4 (accidentally split C-6 into two lanes, but both showed the same results and there was no contamination.) W+ in lane 5, W- in lane 6, H2O control in lane 7. Controls worked.<\/p>\n
Gel electrophoresis, Wolbachia<\/em> 16S: DNA ladder in lane 1, C-5 in lane 2, C-6 in lane 3, W+ in lane 4, W- in lane 5, H2O control in lane 6. No mistakes. Controls worked.<\/p>\n
The primer did not bind correctly for DNA sequencing, the quality scores were not high enough to blast. However, we are able to tell that it is a Spotted Lantern Fly because of its morphology and local knowledge.<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/C6CO1-CO1_SLF_43F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/20250926_145416-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/20250926_145548-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/20250926_145637-scaled.jpg,",38.9866412000,-77.0615970000,09/15/2025,"
\n
Terrestrial<\/li>\n
79 degrees out<\/li>\n
Wild-caught during end of summer<\/li>\n
Color is brownish-black<\/li>\n
4+ legs<\/li>\n
Very rotund<\/li>\n<\/ul>\n",Armadillidium vulgare,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Image-10-16-25-at-10.02 AM.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-04-at-1.36.31 PM.png,","
DNA Extraction<\/p>\n
\n
The arthropod crushed up easily<\/li>\n<\/ul>\n
PCR<\/p>\n
\n
Everything went as planned<\/li>\n<\/ul>\n
Gel Electrophoresis, Arthropod CO1<\/p>\n
Image 1:<\/p>\n
\n
Lane 1 = DNA LADDER<\/li>\n
Lane 2 = A-1<\/li>\n
Lane 3 = A-2<\/li>\n
Lane 4 = A-3<\/li>\n
Lane 5 = A-4<\/li>\n
Lane 6 = Wolbachia (+)<\/li>\n
Lane 7 = Wolbachia (-)<\/li>\n
Lane 8 = H20<\/li>\n<\/ul>\n
<\/p>\n
Image 2:<\/p>\n
\n
Lane 1 = DNA LADDER<\/li>\n
Lane 2 = A-5<\/li>\n
Lane 3 = A-6<\/li>\n
Lane 4 = Wolbachia (+)<\/li>\n
Lane 5 = Wolbachia (-)<\/li>\n
Lane 6 = H20<\/li>\n<\/ul>\n
Gel Electrophoresis, Wolbachia<\/p>\n
\n
Everything went as planned, our controls worked<\/li>\n<\/ul>\n
<\/p>\n
\n
Lane 1 = DNA LADDER<\/li>\n
Lane 2 = A-1<\/li>\n
Lane 3 = A-2<\/li>\n
Lane 4 = A-3<\/li>\n
Lane 5 = A-4<\/li>\n
Lane 6 = Wolbachia (+)<\/li>\n
Lane 7 = Wolbachia (-)<\/li>\n
Lane 8 = H20<\/li>\n<\/ul>\n
<\/p>\n",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/A3CO1-CO1F_R-2.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_4251.jpeg,",42.4596491252,-73.3152805609,10/08/2025,"
When I collected the bug it was stuck in a spider web, however it was still alive.<\/p>\n",Hemiptera,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_4853.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_4852.jpeg,","
1) We used a DNA extraction protocol based on the insect adaptation of New England Biolabs\u2019 Monarch Spin DNA Extraction kit (Product # T3010)<\/p>\n
2) The specimen was incubated for 40 minutes in hot water bath at 56 degrees C.<\/p>\n
3) Details that differed from the written protocol were the change in pipette tips. During Cell Lysis when I was adding 10ul of PK to each tube, I completely forgot to switch out the pipette tips.<\/p>\n
Our first PCR reaction was set up on 10\/16\/25 and was a duplex reaction because we both used both Arthropod CO1 and Wolbachia 16S primers together. An annealing temperature of 49 degrees Celsius was used. This Taq polymerase: New England Biolabs One Taq Hot Start Quick-Load 2X Master-Mix with Standard Buffer (#M0488S) was used.<\/p>\n
\n
Our first gel picture was 10\/30\/25<\/li>\n
It was run at 125 volts for 30 minutes<\/li>\n
Use New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product #N0559S)<\/li>\n<\/ul>\n
\n
Second PCR reaction 10-30-25<\/li>\n
Used Taq polymerase as the first PCR reaction<\/li>\n
Single with the Wolbachia primer<\/li>\n
Annealing temp – 55 degrees Celsius<\/li>\n
Made sure to switch out each pipette tip this time<\/li>\n<\/ul>\n
<\/p>\n
\n
Our second gel image was taken on 11\/6\/25<\/li>\n
It was run at 125 volts for 30 minutes<\/li>\n
Use New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product #N0559S)<\/li>\n<\/ul>\n",No,,,, "",37.8043856000,-122.4210043000,09/08/2025,"
Common fruit fly, slender body, yellowish color, caught near underbrush.<\/p>\n",Diptera,BABEC,Rear half,MiniPCR,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Screenshot-2025-10-14-11.50.45-AM-1.png,","
protocol<\/a><\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_8832.jpeg,",42.6206584587,-71.3751268062,09/10/2025,"",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Screenshot-2025-10-15-10.49.26-AM-4.png,","
Ran gel electrophoresis for 20 mins<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-140-scaled.jpg,",41.9295355,-88.74991279999999,09/19/2024,"
millipede<\/p>\n",Polydesmida,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-139-scaled.jpg,",41.9295355,-88.74991279999999,10/10/2024,"
rove beetle<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-138.jpg,",41.9295355,-88.74991279999999,08/14/2024,"
beetle<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-137-scaled.jpg,",41.9295355,-88.74991279999999,08/06/2024,"
earwig<\/p>\n",Dermaptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-136.jpg,",41.9295355,-88.74991279999999,08/06/2024,"
spider<\/p>\n",Araneae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-135-scaled.jpg,",41.9295355,-88.74991279999999,10/10/2024,"
picnic beetle<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-134-scaled.jpg,",41.9295355,-88.74991279999999,10/10/2024,"
ant<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-133-scaled.jpg,",41.9295355,-88.74991279999999,10/10/2024,"
wasp<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-131-scaled.jpg,",41.9295355,-88.74991279999999,10/10/2024,"
moth<\/p>\n",Lepidoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-130-scaled.jpg,",41.9295355,-88.74991279999999,07/30/2024,"
rove beetle<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-129-scaled.jpg,",41.9295355,-88.74991279999999,07/23/2024,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-128-scaled.jpg,",41.9295355,-88.74991279999999,07/30/2024,"
shield bug<\/p>\n",Hemiptera,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-127-scaled.jpg,",41.9295355,-88.74991279999999,07/29/2024,"
cicada<\/p>\n",Hemiptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-126-scaled.jpg,",41.9295355,-88.74991279999999,08/14/2024,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-125-scaled.jpg,",41.9295355,-88.74991279999999,08/14/2024,"
rove beetle<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-124-scaled.jpg,",41.9295355,-88.74991279999999,07/23/2024,"
beetle<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-123-scaled.jpg,",41.9295355,-88.74991279999999,09/26/2024,"
wasp<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-120-1-scaled.jpg,",41.9295355,-88.74991279999999,09/12/2024,"
rove beetle<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-119-scaled.jpg,",41.9295355,-88.74991279999999,07/23/2024,"
ant<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-118-1-scaled.jpg,",41.9295355,-88.74991279999999,08/20/2024,"
grasshopper<\/p>\n",Orthoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-117-1-scaled.jpg,",41.9295355,-88.74991279999999,08/14/2024,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-116-1.jpg,",41.9295355,-88.74991279999999,08/09/2024,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-117-scaled.jpg,",41.9295355,-88.74991279999999,08/14/2024,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-116.jpg,",41.9295355,-88.74991279999999,08/14/2024,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-115-scaled.jpg,",41.9295355,-88.74991279999999,08/14/2024,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-114-scaled.jpg,",41.9295355,-88.74991279999999,10/03/2024,"
ant<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-113-scaled.jpg,",41.9295355,-88.74991279999999,10/13/2024,"
spiderara<\/p>\n",Araneae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-112-scaled.jpg,",41.9295355000,-88.7499128000,10/03/2024,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-109-scaled.jpg,",41.9295355,-88.74991279999999,10/03/2024,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-108-scaled.jpg,",41.9295355000,-88.7499128000,10/03/2024,"
ant<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-107-scaled.jpg,",41.9295355,-88.74991279999999,08/14/2024,"
ant<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-106.jpg,",41.9295355,-88.74991279999999,09/12/2024,"
fruit fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-105-scaled.jpg,",41.9295355,-88.74991279999999,10/03/2024,"
wasp<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-30-1-scaled.jpg,",41.9295355,-88.74991279999999,08/14/2024,"
green bottle fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_1689-scaled-e1758738483766.jpg,",37.8044,-122.421,09/05/2025,"",Culicidae,BABEC,Abdomen,MiniOne,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_7555-1.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3336.jpeg,",36.1444896,-86.8011462,,"",,,,,,,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-31-scaled.jpg,",41.9295355,-88.74991279999999,07/14/2024,"
japanese beetle<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-29-scaled.jpg,",41.9295355,-88.74991279999999,10/03/2024,"
daddy long legs<\/p>\n",Opiliones,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-28-.jpg,",41.9295355,-88.74991279999999,09/12/2024,"
spider<\/p>\n",Araneae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-27-.jpg,",41.9295355,-88.74991279999999,09/12/2024,"
spider<\/p>\n",Araneae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-26-.jpg,",41.9295355,-88.74991279999999,07/24/2024,"
spider<\/p>\n",Araneae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-24.jpg,",41.9295355,-88.74991279999999,09/12/2024,"
picnic beetle<\/p>\n",Coleoptera,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-25-1-1-scaled.jpg,",41.9295355000,-88.7499128000,09/12/2024,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-23.jpg,",41.9295355,-88.74991279999999,09/12/2024,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/NIU-22.jpg,",41.9295355,-88.74991279999999,08/14/2024,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Fruit-Fly-Drosophilidae-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_2207-1-1.jpg,",42.4756600000,-73.2023500000,10/02/2025,"
Residential backyard, near some blueberry bushes and a tree stump. By a garden with some rotting tomatoes on the vine.<\/p>\n",Drosophila suzukii,Monarch DNA extraction (NEB),Whole arthropod,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_2207-1-2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_2350-scaled.jpeg,","
We used a DNA extraction protocol based on the insect adaptation of New England’s biolabs’ Monarch Spin gDNA Extraction kit.<\/p>\n
the specimen was incubated for 30 minutes in a hot water back at 56 degrees C.<\/p>\n
Our first Gel image was taken on 10\/23\/2025. It was run at 125 volts for 29 minutes. The DNA Ladder used was New England Biolabs 1kb Plus DNA Ladder for Safe Stains (product#N0559S<\/p>\n
We ran a second PCR\u00a0 – this time a single PCR and not a duplex – on 11\/5\/2025. A second gel electrophoresis was run on 11\/6\/2025. It was run at 125 volts for 25 minutes. The DNA Ladder used was New England Biolabs 1kb Plus DNA Ladder for Safe Stains (product#N0559S).<\/p>\n",No,GAGCTTGAGCCGGAATAGTGGGAACATCTCTAAGAATTTTAATTCGAGCTGAATTAGGTCATCCAGGAGCATTAATTGGAGATGACCAAATTTATAACGTAATTGTTACCGCACATGCTTTTATTATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGATTTGGAAATTGATTAGTTCCATTAATATTAGGGGCTCCAGATATAGCATTCCCACGAATAAATAATATAAGATTTTGACTACTGCCCCCTGCTCTTTCTTTATTATTAGTGAGAAGAATGGTTGAAAACGGAGCTGGAACAGGTTGAACTGTTTACCCACCTCTTTCTGCTGGAATTGCTCATGGAGGGGCTTCAGTAGATTTAGCAATTTTTTCATTACATCTAGCCGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACAACTGTAATTAATATACGATCAACGGGTATTACATTAGACCGAATACCTTTATTTGTTTGATCAGTTGTAATTACTGCTTTATTACTTTTATTATCATTACCAGTACTAGCTGGAGCTATTACTATATTATTAACAGATCGAAATTTAAACACCTCATTTTTTGACCCGGCAGGAGGAGGGGATCCTATTTTATATCAACATTTATTTTGA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/20251001_124019-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/20251001_124054-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/20251001_124028-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/20251001_124210-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/20251001_124936-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/20251001_125010-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/20251007_130758-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/20251007_130806-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/20251007_130821-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/20251007_130854-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Wolbachia-PCR-RD-2-results-2-1.jpg,",42.4520310000,-73.2251241000,09/29/2025,"
Very warm and dry autumn evening with a temperature of 65 F. Specimen was collected in a plastic tube in the backyard of an urban, but park-like setting. Specimen was immediately placed in freezer to best preserve specimen.<\/p>\n",Pholcus phalangioides,Monarch DNA extraction (NEB),Abdomen,MiniOne,lysis,New England Bio Labs 1kb Plus DNA Ladder for safe stains,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Wolbachia-PCR-RD-2-results-2.jpg,","
We followed the protocol, with the exception of making a pipetting error on the positive control sample.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/tempImagew0gXyI-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/tempImageRH6Y8K-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/tempImage8xL4HE-scaled.jpg,",42.1568847000,-73.0538097000,09/26/2025,"
The arthropod was found under a rock initially rolled into a ball. Once the specimen was submerged into alcohol it unrolled.<\/p>\n",Armadillidae,Monarch DNA extraction (NEB),Partial abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_5916.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_5974.jpg,","
\n
We had used a DNA extraction protocol based on the insect adaptation of New England Biolabs’ Monarch Spin gDNA kit (Product # T3010).<\/li>\n
The specimen was incubated for 30 minutes in a hot water bath at 56 degrees C.<\/li>\n
During our lab experiement, we had made one accidental mistake that made no difference in our results, but we had dropped our spin columns in the discard thinking we didnt need them and later realized we needed them for the end part of DNA Elution.<\/li>\n
Our PCR reaction was set up on 10\/16\/25 and: was a duplex reaction because we used both the Arthropod CO1 and Wolbachia 16S primers together.<\/li>\n
Taq Polymerase: New England Biolabs OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer (#M0488S) was used.<\/li>\n
Annealing temperature of 49 degrees Celsius.<\/li>\n
Our first Gel image was taken on 10\/23\/25 and: was run at 125 volts for 30 minutes.<\/li>\n
New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S).<\/li>\n
Our second PCR Reaction was set up on 10\/30\/25, used same Taq Polymerase as the first PCR reaction:<\/li>\n
was a duplex reaction that used the Wolbachia and Arthropod primers.<\/li>\n
used annealing temperature of 49 degrees Celsius.<\/li>\n
Our second Gel image was taken on 11\/6\/25 and was run at 125 volts for 30 minutes.<\/li>\n
Used this DNA Ladder: New Biolabs 1kb Plus DNA Ladder for Safe Stains (product #N0559S).<\/li>\n<\/ul>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/1000003212-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/1000003210-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/1000003208-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/1000003206-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/1000003204-scaled.jpg,",42.4680654000,-73.2039099000,10/01/2025,"
Arthropod was located in the soil underneath some stacked wood and was very active with several others alike; it was approximately 5pm in the afternoon and 63 degrees Fahrenheit outside on a sunny and clear day.<\/p>\n",Armadillidae,Monarch DNA extraction (NEB),Partial abdomen,,,,"","
We used a DNA extraction protocol based on the insect adaptation of New England Biolabs’ Monarch DNA Extraction kit (Product # T3010).<\/p>\n
<\/p>\n",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_7362-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_7369-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_7370-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_7364-scaled.jpeg,",42.4597400000,-73.3150700000,09/26/2025,"
I found the bee pollinating on campus, and I threw a ziplock bag over it. I caught it at 10:25 am, and kept it preserved in alcohol. for 5 days. On day 6; I brought it to lab.<\/p>\n",Bombus impatiens,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"","
We may have had a pipette error, so we will try again to get a more accurate reading.<\/p>\n
Because I am not able to create a photo with description, I will write down what each lane is.<\/p>\n
Lane#1= DNA Ladder<\/p>\n
Lane#2= AT<\/p>\n
Lane#3= VXRP<\/p>\n
Lane#4= +Arthropod Control<\/p>\n
Lane#5= -Arthropod Control<\/p>\n
Lane#6= Water<\/p>\n
Lane#7= +DNA Control<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/image8-rotated-e1759336646136.jpeg,",42.4515033259,286.7524328035,09/26/2025,"
Hairy, hides in shadows, small<\/p>\n",Salticidae,Monarch DNA extraction (NEB),Whole arthropod,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_4127.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_4128-2.jpeg,","
1. we used a DNA extraction protocol based on the insect adaption of New England Biolabs\u2019 Monarch Spin gDNA Extraction kit<\/p>\n
2. the specimen was incubated for 30 minutes in a hot water bath at 56 degrees C<\/p>\n
3. Details that differed from the written protocol were in step 16 when liquids were uneven<\/p>\n
LAB 7<\/p>\n
1. Our first PCR reaction was set up on 10\/15\/2025<\/p>\n
\n
was a duplex reaction because we used both the Arthropod CO1 and Wolbachia 16S primers together.<\/li>\n
used an annealing temperature of 49 degrees Celsius<\/li>\n
used this raw polymerase: New England Biolabs OneTaq Hot Start Quick-Load 2X Master Mic with Standard Buffer (#M0488S)<\/li>\n
no mistakes, time differences, etc<\/li>\n<\/ul>\n
LAB 8<\/p>\n
\n
Our first Gel image was taken in 10\/22\/2025<\/li>\n
Ran at 125 volts for 23 minutes<\/li>\n
Used this DNA ladder: New England Biolabs 1kb Plus DNA Ladder for Safe Stains<\/li>\n<\/ul>\n
Lab 9 10\/28<\/p>\n
– Our second PCR reaction was set up on 10\/29\/2025, used the same Taq polymerase as the fist PCR reaction and:<\/p>\n
\n
Was a single reaction thwt used the arthropod CO1 primer<\/li>\n
Used an annealing temperature of 49 degrees celsius<\/li>\n<\/ul>\n
Lab 10<\/p>\n
\n
gel electrophoresis system: edvotek gel electrophoresis<\/li>\n
buffer: 1X TAE<\/li>\n
DNA stain: SYBR Safe<\/li>\n
our second gel image was taken on 11\/05\/2025<\/li>\n
ran at 125 volts for 23 minutes<\/li>\n
used this DNA ladder: new england biolabs 1kb plus DNA ladder for safe stains<\/li>\n<\/ul>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_5978-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_5976-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_5974-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_5973-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_5972-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_5971-scaled.jpeg,",42.4653622000,-73.2489147000,09/30/2025,"
Collected around 06:30 pm outside on the east side of the collector\u2019s home. The arthropod was observed walking quickly along a brick walkway.<\/p>\n",Lasius neoniger,Monarch DNA extraction (NEB),Whole arthropod,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/GEP2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-05-at-1.12.02 PM.png,","
\n
Used a DNA extraction protocol based on the insect adaptation of New England Biolabs’ Monarch Spin gDNA Extraction kit (Product #T3010).<\/li>\n
The specimen was incubated for 42.38 minutes in a hot water bath at 56 degrees C.<\/li>\n<\/ul>\n
<\/p>\n
Our first PCR was set up on 10\/15\/25 and:<\/p>\n
\n
was a duplex reaction because we used both the arthropod CO1 and Wolbachia 16S primers together<\/li>\n
used an annealing temperature of 49 degrees Celsius<\/li>\n
used this Taq polymerase: New England Biolabs OneTaq Hot Start Quick-Load 2X Master Mis with Standard Buffer (#M0488S)<\/li>\n<\/ul>\n
Our first gel image was taken on 10\/22\/25 and<\/p>\n
\n
was run at 125 volts for 24 minutes<\/li>\n
used this DNA ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N05595S)<\/li>\n<\/ul>\n
Our second PCR was set up on 10\/29\/25, used the same Taq polymerase as the first PCR reaction, and:<\/p>\n
\n
was a Single reaction that used the Arthropod CO1 primer.<\/li>\n
used an annealing temperature of 49\u00b0 Celsius.<\/li>\n
Differed from the standard protocol in that when we mixed the PCR cocktail, we mistakenly used the amount of water used in a Duplex PCR reaction so we ran out of PCR cocktail and had to mix a single batch of PCR cocktail for the water control which ended being short. The source of the error was identified afterward.<\/li>\n<\/ul>\n
Our second Gel image was taken on 11\/5\/25 and:<\/p>\n
\n
was run at 125 volts for 23 minutes.<\/li>\n
used this DNA ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S).<\/li>\n<\/ul>\n",No,GAGCTGGTATAATCGGCTCATCTATAAGAATAATTATTCGTCTAGAATTAGGATCATCTAATTCATTGATTAATAATGATCAAATTTATAATTCTATAGTTACAAGACATGCATTCATTATAATTTTCTTTATAGTTATGCCTTTCATAATTGGAGGATTTGGAAATTTCCTTGTACCTTTAATATTAGGCTCACCTGATATAGCTTATCCACGTATAAACAATATGAGATTTTGACTTTTACCCCCCTCAATTTCTCTTCTTATTTTAAGAAATTTTATTAATGATGGTGTTGGGACAGGATGAACTGTTTATCCTCCTTTAGCTTCAAATATTTTCCATAATGGACCTTCAGTTGATTTAACTATTTTTTCCCTTCATATTGCTGGTATATCTTCAATTTTAGGAGCTATTAATTTTATTTCAACTATCTTAAATATACATCACAAAAATTTTTCTATTGATAAAATCCCTTTACTTGTATGATCAATTTTAATTACTGCAATTTTACTTCTCTTATCCTTACCCGTTCTTGCAGGAGCTATTACTATACTTTTAACTGACCGTAATCTTAACACTTCATTTTTTGATCCATCAGGTGGCGGAGATCCTATTTTATATCAACATCTTTTTTGA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/HannaFCO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/processed-F98E35D6-CF40-4C8C-A454-0F89887A105E-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/processed-92612937-5504-4684-BE1C-E84346F7486F.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/processed-441A1D14-D5F0-466F-B2D7-1DA57A39A49E.jpeg,",42.4550185000,-73.1858763000,09/26/2025,"
I found it flying around in my house.<\/p>\n",Maladera formosae,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/unnamed.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/unnamed-2.jpg,","
We used a DNA extraction protocol based on the insect adaptation of New England Biolab’s Monarch Spin gDNA Extraction kit. (Product #T3010)<\/p>\n
The specimen was incubated for 30 minutes but checked every 15 minutes to vortex.<\/p>\n
Our first PCR reaction was set up on 10\/14\/25 and was a duplex reaction because we used both the arthropod C01 and Wolbachia 16s primers together. Then used an annealing temperature of 49 degrees Celsius. we used this Taq polymerase: New England Biolab’s OneTaq Hot Start Quick load 2X Master Miz with Standard Buffer (#M0488S)<\/p>\n
<\/p>\n
Our first gel image was taken on 10\/21\/25 and was run at 125 volts for 20 to 25 minutes, Used DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (products # N0559S)<\/p>\n
<\/p>\n
Our second PCR reaction was set up on 10\/28\/25, used the same Taq polymerase as the first PCR reaction and was a single reaction that used the Wolbachia F,R primers. Used an annealing temperature of 55 degrees Celsius.<\/p>\n
<\/p>\n
Our second gel was taken on 11\/4\/25 and was run at 125 volts for 20 to 25\u00a0 minutes. Used this DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (products # N0559S)<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_2428-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_2450-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_2418-scaled.jpeg,",42.4732238000,-73.1546342000,09/29/2025,"
The fly was found inside a screened in porch. It was flying around the porch and kept landing on the screen.<\/p>\n",Pollenia rudis,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-04-at-1.09.16 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-04-at-1.04.49 PM.png,","
1.We used a DNA extraction protocol based on the insect adaptation of New England Biolabs’ Monarch Spin gDNA Extraction Kit(Product # T3010).<\/p>\n
2. The specimen was incubated for 30 minutes in a hot water bath at 56 degrees C<\/p>\n
\u2022 Our first PCR reaction was set up on October 14, 2025 and:<\/p>\n
\u2022was a duplex reaction because we used both Arthropod CO1 and Wolbachia 16S primers together<\/p>\n
\u2022used an annealing temperature of 49 degrees Celsius.<\/p>\n
\u2022used the Taq polymerase: New England Biolabs OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer(#M0488S)<\/p>\n
\n
Our first Gel image was taken on 10\/21\/25 and: was run at 250 volts for 21 minutes .<\/li>\n
Used this DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains(product # N0559S)<\/li>\n<\/ul>\n
\n
ur <\/span>second<\/b> PCR reaction was set up on 10\/28\/25 , used the same Taq polymerase as the first PCR reaction, and:\u00a0<\/span><\/li>\n
was a single reaction that used, <\/span>the wolbachia primer(s).<\/li>\n
used an annealing temperature of 55 degrees Celsius.<\/span><\/li>\n<\/ul>\n
Our second gel image was taken on 11\/4\/25 and: was run at 125 volts for 25 minutes.<\/p>\n
\n
used this DNA ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S).<\/li>\n<\/ul>\n",Yes,GATCAGGAATAGTAGGAACCTCTCTAAGAATTTTAATTCGAGCAGAACTAGGACATCCTGGAGCATTAATTGGCGATGATCAAATTTATAATGTAATTGTAACAGCTCATGCCTTTGTTATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGAAATTGACTTGTCCCTTTAATACTAGGAGCACCTGATATAGCATTTCCTCGAATGAATAATATAAGTTTTTGACTTTTACCTCCTGCACTTACACTACTATTGGTGAGCAGTATAGTGGAAAACGGAGCTGGGACAGGATGAACTGTTTACCCTCCTCTATCTTCTAATATTGCTCATGGAGGAGCTTCTGTTGATTTAGCTATCTTTTCCCTTCATTTAGCAGGAATTTCTTCAATTTTAGGAGCAGTTAATTTTATTACAACTGTTATTAATATGCGATCTACAGGAATTACATTTGACCGAATACCTTTATTTGTTTGATCTGTAGTAATTACAGCATTATTACTCCTTTTGTCCTTACCTGTATTAGCAGGAGCTATTACTATATTATTAACAGATCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGAGGAGGTGATCCAATTTTATACCAACATTTA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_6390-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_6391-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_6392-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_6389-scaled.jpeg,",42.4500845000,-73.2453824000,09/30/2025,"
Found on my windowsill surrounded by leave debris.<\/pre>\n",Cylisticus convexus,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/thumbnail_image0.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/thumbnail_image0-2.jpg,","
We used a DNA extraction protocol based on the insect adaptation of New England Biolabs’ Monarch Spin gDNA Extraction Kit (Product # T3010).<\/p>\n
The specimen was incubated for 30 minutes in a hot water bath at 56 degrees C.<\/p>\n
Our first PCR reaction was set up on 10\/14\/2025 and was a duplex reaction because we used both the Arthropod CO1 and Wolbachia 16S primers together. We used an annealing temperature of 49 degrees Celsius. We used this Taq polymerase: New England Biolabs OneTaq Hot Start Quick Load 2X Master Mix with Standard Buffer (#M0488S)<\/p>\n
Our first gel image was taken on 10\/21\/25 after our first PCR test and was run at 125 volts for 25 minutes. We used New England Biolabs 1 kb Plus DNA Ladder for Safe Staines (product # N0559S)<\/p>\n
Our second second PCR reaction was set p on 10\/28\/25, used the same Taq polymerase as the first PCR reaction, and was a single reaction that used the Wolbachia F, R primers. We used an annealing temperature of 55 degrees Celsius.<\/p>\n
Our second gel image was taken on 11\/4\/25 after our first PCR and was run at 125 volts for 20-25 minutes.\u00a0 We used this DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S)<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_2446-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_9598-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_9599.jpeg,",42.3589613000,-73.2591669000,09/29/2025,"
I found it on a window in warm environment indoors.<\/p><\/blockquote>\n",Pollenia pediculata,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-04-at-1.04.40 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/Screenshot-2025-11-04-at-1.17.42 PM.png,","
\n
We used a DNA extraction protocol based on the insect adaption of New England Biolabs Monarch Spin gDNA Extraction kit(product# T3010)<\/li>\n
The species was incubated for 30 minutes in a hot water bath at 56 degrees Celsius.<\/li>\n<\/ol>\n
\n
Our first PCR reaction was set up on October 14, 2025 and:<\/li>\n
was a duplex reaction because we used both Arthropod CO1 and Wolbachia 16S primers together<\/li>\n
used an annealing temperature of 49 degrees Celsius<\/li>\n
used the Taq polymerase: New England Biolabs OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer(#M0488S)<\/li>\n
Our first Gel image was taken on October 21, 2025 and:<\/li>\n
was run at 250 volts for 21 minutes<\/li>\n
used the DNA ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains(product # N0559S)<\/li>\n
\n
\n
Our second PCR reaction was set up on 10\/28\/2025 , used the same Taq polymerase as the first PCR reaction, and:<\/li>\n<\/ul>\n
\n
was a single reaction that \u00a0wolbachia_F \u00a0primer(s).<\/li>\n<\/ul>\n
\n
used an annealing temperature of \u00a055 degrees Celsius<\/li>\n<\/ul>\n<\/li>\n
Our second Gel image was taken on 11\/04\/2025 and:\n
\n
was run at 125 volts for 25 minutes<\/li>\n
used this DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n",Yes,GATCAGGAATAGTAGGTACCTCTTTAAGAATATTAGTTCGAGCAGAATTAGGGCATCCTGGAGCATTAATCGGTGATGATCAAATTTATAATGTAATTGTAACAGCCCATGCCTTTGTTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGACTTGTTCCTTTAATATTAGGAGCACCTGATATAGCATTCCCTCGAATGAATAATATAAGTTTTTGACTATTACCTCCTGCACTTACATTATTATTGGTGAGCAGTATAGTGGAAAACGGAGCTGGGACAGGATGAACTGTTTACCCACCTCTATCTTCTAATATTGCTCATGGAGGAGCTTCTGTTGATTTAGCTATTTTTTCTCTTCATTTAGCAGGAATTTCTTCAATTTTAGGAGCAGTTAATTTTATTACAACTGTAATTAATATACGATCTACAGGTATTACATTTGACCGAATACCTTTATTTGTTTGATCTGTAGTAATTACAGCATTATTACTTCTTTTATCCTTACCTGTATTAGCAGGAGCTATCACTATATTATTAACAGATCGAAATTTAAATACTTCCTTTTTTGATCCTGCAGGAGGAGGAGACCCAATTTTATACCAACATTTATTTTGA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_2339-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_2342-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_2338-scaled.jpeg,",42.4537000000,-73.2665000000,09/26/2025,"
The moth was still for over 24 hours on my outdoor screen door. thinking it was dead. I put it into my tube and it began to fly around aggressively<\/p><\/blockquote>\n",Papaipema inquaesita,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_2883.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_3278.jpeg,","
We used a DNA extraction protocol based on the insect adaptation of New England Biolabs\u2019 Monarch Spin gDNA Extraction kit (Product # T3010)<\/p>\n
The specimen was incubated for 30 minutes in a hot water bath at 56 degrees C.<\/p>\n
Our second Gel image was taken on 11\/4\/25 and was run at 125 volts for 25 minutes<\/p>\n
used this DNA ladder: New Englands Biolabs 1 kb Plus DNA Ladder for Safe Stains (product #N0559S)<\/p>\n
<\/p>\n
On our second PCR reaction was set up on 10\/28\/25, used the same Taq polymerase as the first PCR reaction, and:<\/p>\n
was a single reaction that used the Arthropod primers<\/p>\n
used an annealing temperature of 49 degrees Celsius.<\/p>\n",No,CAAATTTATAATACTATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCCATTATAATTGGAGGATTTGGAAATTGACTTGTACCTTTAATATTAGGAGCTCCTGATATAGCATTTCCACGAATAAATAATATAAGTTTTTGATTACTTCCACCCTCATTAACTTTATTAATCTCAAGAAGAATTGTAGAAAATGGAGCTGGAACAGGATGAACAGTGTACCCCCCACTTTCATCTAATATTGCTCATGGAGGAAGTTCTGTAGATTTAGCAATTTTTTCCCTTCATTTAGCTGGTATTTCCTCCATCTTAGGAGCTATTAATTTTATTACCACAATTATTAATATACGACTAAATAATTTATCTTTTGATCAAATACCTTTATTTATTTGAGCTGTAGGTATTACTGCATTTTTATTATTACTATCATTACCTGTTTTAGCAGGAGCTATCACAATATTATTAACAGATCGAAATTTAAATACATCATTTTTTGATCCAGCAGGAGGAGGAGACCCAATTTTATACCAACATTTATTTTGAT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_2454-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_6352.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_2455-scaled.jpeg,",42.2112097396,-73.3458149607,09/26/2025,"
It was outside near the corner of the building in moist dirt near leaves and rocks.<\/p>\n",Armadillidae,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_6416.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_6468.jpeg,","
Our first PCR reaction was set up on 10\/14\/25 and was a duplex reaction because we used both the Arthropod CO1 and Wolbachia 16S primes together. We used an annealing temperature of 49 degrees celsius. We used this Taq polymerase: New England Biolabs OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer (#M0488S).<\/p>\n
<\/p>\n
We used a DNA extraction protocol based on the insect adaptation of New England Biolabs Monarch Spin gDNA Extraction kit. (Product #T3010).<\/p>\n
The specimen was incubated for 30 minutes in a hot water bath at 56 degrees C.<\/p>\n
<\/p>\n
Our second PCR reaction was set up on 10\/28, we used the same Taq polymerase as the first PCR reaction and it was a duplex reaction that used Arthropod C01 and Wolbachia 16S primers. We used an annealing temperature of 49 degrees celsius.<\/p>\n
Our second gel image was taken on 11\/4\/25 and was run at 125 volts for 25 minutes. We used the New England Biolabs 1 kb Plus DNA Ladder for safe stains (product #N0559S)<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_2341-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_7826-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_7243-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_8034.jpeg,",42.4379573,-73.2074802,09/29/2025,"
When capturing My arthropod I noticed it was wrapped in Web.<\/p>\n",Curculionidae,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_8034-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/IMG_7978-1.jpeg,","
We used a DNA extraction protocol based on the insects adaption of New England biolabs\u2019 monarch spin gDNA extraction kit (product #T3010)<\/p>\n
The specimen was incubated for 30 minutes in a hot water bath at 56\u00b0 C<\/p>\n
our first gel image was taken on October 21 and ran at 125 V for 20 minutes.we Used the DNA ladder : New England bio labs 1KB plus DNA ladder for safety stains product. #N0559S<\/p>\n
our second image was taken on November 4, 2025 and was running out 125 V for 25 minutes. We use this DNA ladder New England bio labs 1KB plus DNA ladder for safety stains product.# N0559S<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_3230-scaled.jpeg,",37.80422564022223,-122.42103726870333,09/09/2025,"
It’s habitat seems to be that of dark damp areas under bushes rocks, and surrounding plants.<\/p>\n",Diplopoda,BABEC,Partial abdomen,MiniPCR,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/Screenshot-2025-09-24-11.14.05-AM.png,","
Used BABEC protocols.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/F1E95509-FB71-46CA-93B0-5B9BC1366150.jpeg,",37.8035481,-122.4240267,09/08/2025,"",Lepidoptera,BABEC,Abdomen,MiniOne,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/Screenshot-2025-09-24-at-11.23.41 AM.png,","
Taq polymerase used<\/p>\n
Annealing temperature(s) for each PCR reaction – 55 degrees C<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_1689-scaled-e1758738483766.jpg,",37.7533388182,-122.4865432878,09/09/2025,"
-about 2cm mosquito, has long legs and antennas, female mosquito<\/p>\n",Culex pipiens,BABEC,Whole arthropod,MiniOne,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/Screenshot-2025-09-24-at-11.18.56 AM.png,","
1. Student Protocol_ Wolbachia PCR<\/a><\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_11F99FEB-1E98-4288-A67E-C7B0D75988A4-scaled.jpeg,",40.5593540000,-75.4028623000,09/11/2025,"
$\"\"$ $\"\"$ $\"\"$ <\/p>\n",Arthropoda,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/9181F88D-3173-4318-9EE3-11F1F9888CF7.jpeg,","",Yes,TGATCNGGAATAATNGGATCATCAATAAGATTATTAATTCGAATAGAACTTAGACATCCAGGAATA TGAATTAATAATGATCAAATTTATAATTCATTAGTTACTAGACATGCATTTTTAATAATTTTTTTTATAGTTATACCATT TATAATTGGAGGATTTGGAAATTATTTAATTCCTTTAATATTAGGATCACCTGATATAGCTTTCCCACGAATAAATAATA TTAGATTTTGATTATTACCTCCATCTATTTTTATATTATTATTAAGAACTTTATTTACACCTAATGTAGGAACAGGTTGA ACTGTATATCCCCCTTTATCATCTTATTTATTTCATTCATCACCTTCAGTAGATATTGCAATTTTTTCTTTACATATAAC AGGAATTTCTTCTATTATTGGTTCATTAAATTTTATTGTTACNATTATATTAATAAAAAATTTTTCATTAAATTATGATC AAATTACTTTATTTTCTTGATCTGTATGTATTACAGTNTTATTATTAATTTTATCATTACCAGTTTTAGCTGGAGCAATT ACTATACTTCTTTTTGATCGAAATTTTAATACATCATTTTTTGATCCAATAGGAGGTGGAGATCCAATTTTATATCAACA TTTATTTTGATTTTTTG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/unnamed.jpg,",40.5593540000,-75.4028623000,09/11/2025,"
Small and furry with a brown body. Two sections of body and light stripes. Has four eyes. Was hiding under a tree and kept trying to rush into the mulch around the trees when caught.<\/p>\n",Trochosa ruricola,Blood and Tissue kit,Abdomen,MiniPCR,TBE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_1996-scaled.jpg,","
DNA extraction: I took just the abdomen of my specimen and made sure to crush it well in the tube before combining it with the difference chemicals.<\/p>\n
Gel electrophoresis for CO1 arthropod PCR:<\/p>\n
1 – DNA Ladder<\/p>\n
2 – Spider specimen<\/p>\n
3 – Bee specimen<\/p>\n
4 – Wolbachia positive control<\/p>\n
5 – Wolbachia negative control<\/p>\n
6 – DNA control<\/p>\n
7 – Water<\/p>\n
Analysis: All of our wells seemed to take well and show up good. However, I don’t think the positive control is showing up correctly. This may be due to a mess up in the extraction process or loss of DNA.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/1000003919-scaled.jpg,",40.5593540000,-75.4028623000,09/11/2025,"
Found in a flower bush in a garden near a forested area.<\/p>\n
Looks like a type of bee: 2 wings, 6 legs, 2 eyes, fuzzy yellow and black upper body, large smooth black lower body.<\/p>\n",Hymenoptera,Qiagen Blood and Tissue kit,Abdomen,Standard electrophoresis system,1X TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/1000004765.jpg,","
Lane 1: DNA Standard<\/p>\n
Lane 2:\u00a0 Arthropod #1 Wol + COI1 \/ MQAM03A+W1<\/p>\n
Lane 3: Arthropod #2 Wol + COl1 \/ MQAM03A+W2<\/p>\n
Lane 4: Wol Pos Wol + COl1 \/ MQAM03Pos<\/p>\n
Lane 5: Wol Neg Wol + COl1 \/ MQAM03Neg<\/p>\n
Lane 6: gDNA control Wol + COl1 \/ MQAM03DNAC<\/p>\n
Lane 7: water control Wol + COl1 \/ MQAM03Water<\/p>\n
Lane 8:\u00a0 EMPTY<\/p>\n
Lane 9: Arthropod #1 Wol + COI1 \/ SGGS03AtW1<\/p>\n
Lane 10: Arthropod #2 Wol + COl1 \/ SGGS03AtW2<\/p>\n
Lane 11: Wol Pos Wol + COl1 \/ SGGS03Pos<\/p>\n
Lane 12: Wol Neg Wol + COl1 \/ SGGS03Neg<\/p>\n
Lane 13: gDNA control Wol + COl1 \/ SGGS03DNA<\/p>\n
Lane 14: water control Wol + COl1 \/ SGGS03Water<\/p>\n",No,TCGNATCATCAATAAGATTATTAATTCNAATANAACTTAGACATCCAGGA ATATGAATTAATAATGATCAAATTTATAATTCATTAGTTACAAGACATGCATTTTTAATAATTTTTTTTATAGTTATACC ATTTATAATTGGAGGATTCGGAAATTATTTAATTCCTTTAATATTAGGATCACCTGATATAGCTTTTCCACGAATAAATA ATATTAGATTTTGATTATTACCCCCATCTATTTTTATATTATTATTAAGAACTTTATTTACACCTAATGTAGGAACAGGT TGAACCGTATATCCCCCTTTATCATCTTATTTATTTCATTCATCACCTTCAGTAGATATTGCAATTTTTTCTTTACATGT AACAGGAATTTCTTCTATTATTGGTTCATTAAATTTTATCGTNGCTATTATATTAATAAAAAATTTTTCATTAAATTATG ATCAAATTACTTTATTTTCATGATCTGNATGTATTACAGTATTATTATTAATTTTATCATTACCAGTTTTAGCAGGAGCA ATTACTATACTTCTTTTTGATCGAAATTTTAATACATCATTTTTTGATCCAATAGGAGGTGGAGATCCAATTNNATATCA ACNTTTTATTTTGANNNTNTTGGTCACCCTGAANNTTAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/512-1-scaled.jpg,",40.5590232000,-75.4028662000,09/11/2025,"
black ant found near tree bottom<\/p>\n",Camponotus,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,1X TAE,SYBR Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_1996-2-scaled.jpeg,","
Used standard Taq polymerase. Three cycles.<\/p>\n
\n
Denaturation (Cycle):<\/strong>\u00a094\u201398\u00b0C for 15\u201330 seconds.<\/span><\/li>\n
Annealing\u00a0(Cycle):<\/strong>\u00a050\u201365\u00b0C for 20\u201340 seconds.<\/span><\/li>\n
Extension\u00a0(Cycle):<\/strong> 68\u201372\u00b0C\u00a0<\/span><\/li>\n<\/ul>\n",Yes,NNNNNNNNNNTTTGCTATTTGATCGGGATAATTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTAGGATCTCCTGA TTCACTAATTCTTAATGATCAAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATAC CTTTTATAATTGGGGGATTTGGTAATTTTTTAATTCCACTTATACTAGGATCTCCTGATATAGCTTACCCTCGTTTAAAT AACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGG TTGAACTATCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATA TTGCTGGTATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTA GATAAAATTCCCTTATTAGTATGATCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGCGC TATTACAATACTACTAACAGACCGAAATCTTAATACTTCATTTTTCGATCCCTCGGGAGGAGGAGATCCTATTTTATACC AACATTTATTTTGATTTTNNNNN,,, "",40.5593540000,-75.4028623000,09/11/2025,"
$\"\"$ $\"\"$ <\/p>\n",Euschistus tristigmus,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,1X TAE,SYBR Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/201-scaled.jpg,","
Procedure for Casting Gels and Loading Samples<\/a><\/p>\n
Gel results are read from lane 1 (bottom-most lane) to lane 8 (top most lane). The results pertinent to this entry (Stinkbug) are in LANE 3.<\/p>\n
Lane 1: DNA Ladder<\/p>\n
Lane 2: Arthropod 1 (Wol + COI1)<\/p>\n
Lane 3: Arthropod 2 (Wol + COI1, Stinkbug Pertinent to THIS entry)<\/p>\n
Lane 4: Wol Pos Wol + COI1<\/p>\n
Lane 5: Wol Neg Wol + COI1<\/p>\n
Lane 6: gDNA control Wol + COI1<\/p>\n
Lane 7: water control Wol + COI1<\/p>\n
Lane 8: EMPTY<\/p>\n",No,GAGCAGGATAGTGGGGTCCGCTATAAGAATTATTATTCGTATTGAATTAGGACAACCAGGA AGATTTATTGGAGATGATCAAATCTATAATGTTGTAGTTACTGCTCACGCATTCGTTATAATTTTTTTTATAGTAATACC AATTATAATTGGAGGATTTGGTAACTGACTAGTGCCATTAATAATTGGAGCTCCTGATATAGCATTCCCTCGAATAAATA ATATAAGATTCTGATTATTACCCCCTTCAATTACTTTATTAATAATAAGTAGATTAGCTGAATCAGGGGCTGGAACCGGC TGAACTGTATACCCCCCACTATCAAGTAATCTATCCCACAGAGGAGCATCAGTAGACTTGGCTATCTTTTCCTTACACTT AGCAGGTGTTTCTTCAATTCTAGGAGCTGTAAATTTCATTTCAACAATTATAAATATACGACCCGAAGGAATAATCCCAG AACGAATCCCCTTATTCGTATGATCAGTAGGAATTACAGCACTATTATTACTACTTTCCCTTCCCGTATTAGCAGGAGCT ATTACTATATTATTAACCGATCGCAACTTTAATACATCCTTCTTTGATCCTTCAGGAGGAGGGGATCCTATCTTGTATCA ACATCTATTCTGATTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_7249.jpg,",40.5593540000,-75.4028623000,09/11/2025,"
It’s gray and small, rolled up, has little legs.<\/p>\n",Armadillidium vulgare,pcr,Whole arthropod,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3552.jpeg,","
Lane 2: MQAM03A+W1<\/p>\n
Lane 3:MQAM03A+W2<\/p>\n
Lane 4:MQAM03Pos<\/p>\n
Lane 5:MQAM03Neg<\/p>\n
Lane 6:MQAM03DNA<\/p>\n
Lane 7: MQAM03water<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_3146.jpg,",40.3394500000,-74.4028623000,09/11/2025,"
6 legs<\/p>\n
around 1 cm<\/p>\n
brown<\/p>\n
line streaks on the back<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,1X TAE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3552-1.jpeg,","
Lane 1: SGSG03W1 –>grasshopper<\/p>\n
Lane 2: SGSG03W2 –> stinkbug<\/p>\n
Lane 3:\u00a0 SGSG03W3 –> positive control<\/p>\n
Lane 4: SGSG03W5 –> negative control<\/p>\n
Lane 5: SGSG03W6 –> DNA control<\/p>\n
Lane 6: SGSG03W6 –> water<\/p>\n",No,TNGANNTNNNNCAGGAATAGTGGGANCTNCNNTAAGTATTATAATTCGAACTGAATTAGGACACCCCG GATCATNATTGGAAATGATCAAATTTATAATGTTGTTGTTACTGCTCATGCCTTCATTATAATTTTTTTTATAGTAATAC CAATTATAATTGGAGGATTTGGTAATTGATTAGTGCCATTAATATTTGGAGCCCCTGATATAGCTTTCCCTCGAATAAAT AATATAAGATTTTGATTATTACCCCCTGCATTAACTCTCCTNATAATAAGTAGAATAATTGAAACCGGAGCTGGAACCGG TTGAACTGTATATCCCCCTTTATCTTCTAATATTGCCCATGGAGGAGCTTCTGTAGATTTGGCTATTTTTTCTTTACATT TAGCAGGAGTTTCTTCAATTTTAGGAGCNGTTAATTTTATTACAACANTTATTAATATACGANCNNNAGGAATTANNTTN GANNNNNNACCNTTATTTGTTNGNNNTGTTGAANTTACNNCTTNNNNTTNNTNNTTATCNTTACCANNACTANNNGGANN TATAACAANNTNATNACCNAACNNAANTTTTANAACTTCTTTTTTGGNCCCCNNAGGAGNNGNAAATCNATTTTGATNTC NNCATTANTCTGANTTTTTNNNCN,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_1664-scaled.jpeg,",40.5590141175,-75.4026647705,09/11/2025,"
Very aggressive and agitated when in captivity; would not stop buzzing.<\/p>\n",Arthropoda,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_1996-1-scaled.jpeg,","
DNA extraction: <\/strong>The abdomen of the bee was crushed as finely as possible. There were some chunks of abdomen left in the sample however.
\nGel Electrophoresis Lanes:<\/strong><\/p>\n
1.) DNA Standard<\/p>\n
2.) Spider sample<\/p>\n
3.) Bee sample<\/p>\n
4.) Positive Wolbachia Control<\/p>\n
5.) Negative Wolbachia<\/p>\n
6.) Positive DNA Control<\/p>\n
7.) Water (Empty)<\/p>\n
<\/p>\n
<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_4659-scaled.jpeg,",40.5593540000,-75.4028623000,09/11/2025,"
Found underneath brush, sedentary when captured<\/p>\n",Carabidae,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,1X TAE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_1996-scaled.jpeg,","",No,TTNNNNGNAGNNTGATNNGGATAGTAGGAACTTCTTTAAGTCTATTAATTCGAGCAGAATTAGGGAATCCAGG ATCACTAATTGGTGATGATCAAATTTATAATGTTATTGTTACTGCCCATGCTTTTATTATAATTTTTTTTATAGTAATAC CTATTATAATTGGTGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCACCTGATATGGCCTTTCCTCGAATAAAT AATATAAGTTTTTGACTTTTACCTCCTTCTTTAACACTTCTTCTTATAAGAAGACTAGTAGAAAGAGGAGCAGGTACAGG TTGAACTGTATACCCCCCTTTATCTTCAGGAATTGCCCATAGAGGTGCTTCAGTAGATTTAGCTATTTTTAGTCTTCACT TAGCGGGGGTATCCTCTATTTTAGGAGCAGTTAATTTTATTACTACTATTATCAATATACGATCAGTAGGTATAACATTT GAACGAATACCACTATTTGTTTGATCAGTAGGTATTACAGCTTTACTATTACTTTTATCATTACCAGTTTTAGCAGGAGC TATTACTATGTTATTAACAGATCGAAATTTAAATACTTCCTTTTTTGATCCTGCAGGAGGGGGAGACCCAATTCTTTACC AACATTTATTTTGATTTTTNG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/1000003911.jpg,",40.5593540000,-75.4028623000,09/11/2025,"
<\/p><\/blockquote>\n",Acanalonia conica,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/20251016_195619-scaled.jpg,","",No,NNNNNNNNNNNNNNNGGTTATGATNNGGAATAANTGGGACANTANTAAGAATAATTATTCGTATTGAATTANCTCAGCCA GGATCANTNATTAAAAATGACCAAATTTATAATTNNATTGTTACNNNACACGCATTTATTATAATCTTTTTTATAGTTAT ACCAATTATAATGGGAGGATGGGGAAACTGACTAGTTCCNATAATAATTGGAGCCCCANATATAGCATTTCCACGAATAA ATAATATAAGATTTTGACNANNACCAATATCCCTATCAATATTAATTATAAGATCAATTTCAGGTTCAGGAGTTGGAACA GGATGAACAGTTTACCCTCCANTATCTAGTCAAANTGCCCATTCAGGACCATCAGTAGATATAGCAATTTTTTCACTTCA TATTGCAGGTATTANNTCAATTATGNNNNNNATTAACTTTATCTCAACAATTTTAAATATACAAACAAAGGGAATAACTA TAGAAAAAACACCACTTTTTTGTTGATCAGTTCTTATTACAGCAGTTTTANNNNTAGGTTCATTACCAGTNTNAGCCGGA GCAATTACAATATTATTAATAGATCGAAACTTCAANACATCATTTTTTGACCCATCAGGAGGGGGGAGATCCAATTCTNT ATCANCATTTATTTTGATTTTTNNNNNNN,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_0054-scaled.jpeg,",40.5593540000,-75.4028623000,09/11/2025,"",Agriphila straminella,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,SYBR Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/1000005230-scaled.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_0253-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_0254-1-scaled.jpeg,",40.5590244424,-75.4026933039,09/11/2025,"",Araneae,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,1X TAE,SYBR Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/04A93BC2-4E01-4843-A0F5-047CA6C58058.jpeg,","
For this DNA extraction, me and my lab partner followed the protocols listed on the wolbachia project website throughout the course of 4 weeks. We went out and collected our species, then they were frozen and we amplified their DNA after extraction from the abdomen. Using different solutions and buffers, we were able to isolate the DNA and using a gel electrophoresis system, we identified that this wolf spider was wolbachia positive.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_1847-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_1849-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_1846-scaled.jpeg,",40.559354,-75.4028623,09/11/2025,"",Lepidoptera,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,1X TAE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/586B617E-9508-4A5C-B799-AE0D635C7AB2.jpeg,","",Yes,GCTGGTATAGTAGGAACTTCTCTAAGATTATTAATTCGAGCAGAATTAGGGAATCCTGGATCT TTAATTGGAGATGATCAAATTTATAATACTATTGTGACAGCTCACGCTTTTATTATAATTTTTTTTATAGTTATACCTAT TATAATTGGAGGATTTGGAAATTGATTAGTACCTTTAATATTAGGAGCCCCAGATATAGCTTTCCCCCGAATAAATAATA TAAGTTTCTGACTTTTACCCCCATCTTTAACATTATTAATCTCTAGAAGAATTGTAGAAAATGGAGCAGGAACAGGATGA ACAGTTTACCCCCCACTTTCATCTAATATTGCCCATGGAGGTAGTTCTGTAGATTTAGCTATTTTTTCTTTACATTTAGC AGGAATTTCTTCTATTTTAGGAGCTATTAATTTTATTACAACAATTATTAATATACGATTAAATAATTTATCTTTTGATC AAATACCATTATTTATTTGAGCAGTAGGTATTACAGCATTTTTATTATTATTATCTTTACCTGTATTAGCTGGAGCTATT ACAATATTATTAACTGATCGAAACTTAAATACATCTTTTTTTGACCCAGCTGGTGGGGGAGACCCTATTCTTTATCAACA TTTATTTTGANTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_4273-scaled.jpeg,",40.5590168077,-75.4026756067,09/11/2025,"",Arthropoda,tweezers,Abdomen,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_4300-scaled.jpeg,","
The researchers collected the arthropods from outside then followed the wolbachia protocol listed on the website.<\/p>\n",No,ATGATNNGGATAATCGGATCATCAATAAGATTATTAATTCGAATAGAACTTAGACATCCAGGAAT ATGAATTAATAATGATCAAATTTATAATTCATTAGTTACAAGACATGCATTTTTAATAATTTTTTTTATAGTTATACCAT TTATAATTGGAGGATTCGGAAATTATTTAATTCCTTTAATATTAGGATCACCTGATATAGCTTTTCCACGAATAAATAAT ATTAGATTTTGATTATTACCTCCATCTATTTTTATATTATTATTAAGAACTTTATTTACACCTAATGTAGGAACAGGTTG AACCGTATATCCCCCTTTATCATCTTATTTATTTCATTCATCACCTTCAGTAGATATTGCAATTTTTTCTTTACATGTAA CAGGAATTTCTTCTATTATTGGTTCATTAAATTTTATCGTTGCTATTATATTAATAAAAAATTTTTCATTAAATTATGAT CAAATTNCTTTATTTTCATGATCTGTATGTATTACAGTATTATTATTAATTTTATCATTACCAGTTTTAGCAGGAGCAAT TACTATACTTCTTTTTGATCGAAATTTTAATACATCATTTTTTGATCCAATAGGAGGTGGAGATCCAATTTTATATCAAC ATTTATTTTGATTTTTTGNC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_3144-1.jpg,",40.5593540000,-75.4028623000,09/11/2025,"
Caught with net in a larger forest area. It was in a bush. Anthropod is green, roughly inch in length. One antenna is visibly broken off. When collected, grasshopper made loud buzzing noise.<\/p>\n",Orthoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,1X TAE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3552.jpg,","
Lane 1: SGGS03W1 -> Grasshopper<\/p>\n
Lane 2: SGGS03W2 -> Stinkbug<\/p>\n
Lane 3: SGGS03W3 -> Positive Control<\/p>\n
Lane 4: SGGS03W4 -> Negative Control<\/p>\n
Lane 5: SGGS03W5 -> DNA Control<\/p>\n
Lane 6: SGGS03W6 -> Water<\/p>\n",No,,,, "",40.559354,-75.4028623,10/26/2025,"
The arthropod was flying around outside on the plain grass field. It was big and brown.<\/p>\n",Lepidoptera,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,1X TAE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/tempImageauowSU-scaled.jpg,","
Made gel, put the DNA sequences in the spots and then allowed the electrophoresis system to run for about 25 minutes. After that we put gel under UV light and examined our bands.<\/p>\n",Yes,GNGCTTGAGCTGGATAGTTGGAACATCTTTAAGAATTTTAATTCGAGCTGAATTAGGACATCCTGGAGCA TTAATTGGAGATGATCAAATTTATAATGTAATTGTAACTGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTAT TATAATTGGTGGATTTGGAAATTGATTAGTGCCTTTAATATTAGGTGCTCCTGATATAGCATTCCCACGAATAAATAATA TAAGATTTTGACTTCTACCTCCTGCTCTTTCTTTACTATTAGTAAGTAGAATAGTTGAAAATGGAGCTGGGACAGGATGA ACTGTTTATCCACCTCTATCCGCTGGAATTGCTCATGGTGGAGCTTCAGTTGATTTAGCTATTTTTTCTCTACATTTAGC AGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACAACTGTAATTAATATACGATCAACAGGAATTTCATTAGATC GTATACCTTTATTTGTTTGATCAGTAGTTATTACTGCTTTATTATTATTATTATCACTTCCAGTACTAGCAGGAGCTATT ACTATATTATTAACAGATCGAAATTTAAATACATCATTTTTTGACCCAGCGGGAGGAGGAGATCCTATTTTATACCAACA TTTATTTTGATT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_8161-scaled.jpg,",40.5589837206,-75.4025718357,09/11/2025,"
when catching the white\/cream color moth it was flying away from us and kept hiding we eventually found it and it was trying to escape the net<\/p>\n",Pieris rapae,qiagen blood and tissue,Abdomen,Standard electrophoresis system,1X TAE,SYBR Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_4756-1-scaled.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/WhatsApp-Image-2025-09-11-at-17.11.41-scaled.jpeg,",40.5589052213,-75.4024958792,09/11/2025,"
It is small and it has a color like light green to white. It has a big wings for its body. It looks like a type of butterfly. The size of the fragment was approximately a few mm in length. They are not harmful to the people, but since they eating the plants, the plant can be weak because of this creature.<\/p>\n",Siphanta acuta,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniOne,1X TAE,SYBR Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/WhatsApp-Image-2025-10-26-at-19.58.30-scaled.jpeg,","
We followed the standard Wolbachia Project protocol to extract DNA and run PCR. After setting up the reactions, we ran the samples on an agarose gel. It was interesting to see how DNA bands appeared under blue light, showing whether our arthropod was infected or not.<\/p>\n",Yes,ATCNGGACTAGTAGGATAATAATAAGAATAATTATTCGAATAGAACTATCTCAACCAGGATCA ATAATCAAAAATGATCAAATTTATAACACAATTGTAACTTCACATGCATTTGTAATAATCTTTTTTATAGTTATACCAAT TATAATTGGTGGTTTTGGAAACTGACTTGTTCCTCTTATAATTGGAGCACCAGATATAGCTTTCCCACGAATAAACAATA TAAGTTTTTGACTTTTACCAATATCAATTACATTATTAATTGCAAGATCAACAGCAGCTTCAGGAACAGGAACAGGATGA ACTGTATACCCACCTCTTTCGGCTCAAGAAGCACATTCAGGACCATCAGTAGATTTAACAATTTTTTCATTACACATTGC AGGTATTAGATCAATTATAGGAGCAATTAATTTTATTTCGACCATTATAAATATGCGAATCAAGGGAATAACAATAGAAA AAACCCCCTTATTTTGTTGATCAGTACTAATTACAGCAATCCTTTTACTAGTTTCTTTACCAGTACTAGCCGGAGCAATC ACAATATTAATTATAGACCGAAATCTAAATACTTCATTTTTTGATCCATCAGGAGGAGGCGATCCTATTCTGTATCAGCA CTTATTCTGATTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_6735-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_6725-scaled.jpeg,",40.5590229685,-75.4026955968,09/11/2025,"
$\"\"$ The arthopod was flying around and sitting on plants every now and then.<\/p>\n",Lepidoptera,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,1X TAE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/gel-scaled.jpeg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_6319-scaled.jpeg,",40.5589810430,-75.4025822793,09/11/2025,"
Characteristics include black and yellow coloration, a continuous yellow band behind the eye that connects to the jaw, and three small black dots on their face.<\/p>\n",Hymenoptera,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,1X TAE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_4706-2-scaled.jpeg,","",No,TGAGCNNNNNCTTTAGGAGCTTCANTNNGAANAATTATNCGNTTAGAATNNNNNNNNNNN GNAGCTTTAATTAATAATGATCAAATTTATAATACTNTTATTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTTAT ACCTTTTTTAGTTGGAGGATTTGGAAATTGATTAATCCCTTTAATATTAGGTGTTCCTGATATAGCATTTCCTCGAATAA ATAATATAAGATTTTGATTATTACCTCCATCCTTATTTTTATTAATCTTAAGAAATTTTATTGGAACCGGAGTAGGGACA GGATGAACTCTGTACCCTCCTTTATCATCAATTGTAGGACATGACTCTCCCTCTGTAGACTTAGGAATTTTTTCTATCCA TATTGCTGGAATTTCATCAATTATAGGTTCAATTAATTTTATTGTTACTATTTTAAATATACACACAAAAACACATTCAC TAAATTTTCTTCCTTTATTTTCATGATCAATTTTAATTACAGCAATTCTTCTCTTGTTATCTCTACCAGTTCTTGCAGGA GCAATTACTATACTTCTTACAGACCGTAATTTAAATACATCTTTCTTTGATCCTGCAGGCGGAGGTGACCCAATTTTATA CCAACATTTATTTTGATTTTTTGGTCACCTGGAAAGTTTAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_2782-rotated.jpg,",40.5583941083,-75.4055741753,09/11/2025,"
Found in a wooded, shaded area, after uncovering a rock, it started to scurry away from the area, and dig deeper into the soil underneath. Once picked, I observed the hard multiple piece shell it had, and many short legs underneath. It was a dark grey in color.<\/p>\n",Armadillidium nasatum,qiagen blood and tissue,Whole arthropod,Standard electrophoresis system,1X TAE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Screenshot-2025-10-26-6.58.51-PM.png,","",No,TTTGGGCAGGGGCTGTAGGTACCGCTTTAAGAATAATTATTCGGACTGAATTAGGGCAGCCTGGGAG TCTCATTGGGGATGATCAAATCTATAATGTAATTGTCACGGCTCATGCTTTTGTAATGATTTTCTTCATAGTAATACCTA TTATAATTGGAGGTTTTGGAAATTGATTAATTCCTTTGATATTAGGGGCTCCTGATATAGCTTTCCCTCGTATAAACAAT ATAAGATTTTGGCTGCTTCCCCCTTCTTTAACTTTACTCCTTAGCAGAGGATTAATTGAAAGAGGAGTAGGAACGGGATG AACTGTTTACCCTCCTTTGGCATCAAGAATTGCCCATAGTGGAGCTTCTGTAGATTTAGGTATCTTTTCTCTTCATCTTG CTGGGGCTTCATCAATTTTAGGGGCAGTAAACTTTATTACGACTATAATTAATATACGGGCGTCCGGAATTAGTATGGAT CGTGTGCCTTTATTTGTTTGGTCTGTTTTAGTAACCGCGGTTCTTCTTCTCTTATCTTTACCTGTTCTTGCTGGAGCTAT TACTATACTATTGACTGACCGTAATTTAAATACATCTTTTTTTGATCCGAGGGGAGGGGGGGATCCTATTCTTTATCAGC ATTTATTCTGATTTTTTG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/1000036677-scaled.jpg,",40.5590240000,-75.4028734000,09/11/2025,"
The insect was flying rapidly around the field.<\/p>\n",Arthropoda,DNeasy (Qiagen),Abdomen,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_7526-scaled.jpeg,",40.5589579956,-75.4025564474,09/11/2025,"
Brown bug, hard shell\/exterior, light brown limbs, pincers at rear abdomen.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,1X TAE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_4706-scaled.jpeg,","",Yes,NNNNNNNNNNNNNNNNGNGNNTNNGNNNNNNAATAGTGGGGACTTCNTTNAGCTTGNTGATTCGGGCAGAANNNNNNNNN NNGGAGCTTTAATCGGAGATGATCAAATTTATAATNNNTNNGTTACGGCCCATGCTTTTGTAATGATTTTTTTTATGGTA ATGCCTATTATGATTGGAGGTTTTGGGAATTGACTGGTACCTTTGATGTTGAGAGCCCCAGATATGGCCTTTCCCCGAAT AAACAACATGAGTTTTTGGTTACTACCCCCTTCGCTTATGCTTTTGCTTTCTGGGAGTATGGTAGATAGAGGAGCTGGTA CAGGTTGGACAGTTTACCCCCCTTTGTCGGGGGCCATCGCCCACGCAGGGGCTTCGGTAGATTTGAGTATTTTTTCATTG CATTTGGCAGGGATTTCATCAATTTTGGGGGCAATTAATTTTATCACAACGGTGATTAATATGCGCCCATCGGGCCTTAA GCTGGAACGAATACCGTTGTTTGTATGATCCGTAGCCATTACTGCTCTTTTATTGTTGCTTTCTTTGCCAGTATTGGCAG GGGCTATCACTATGCTTTTGACGGACCGGAACTTAAATACATCTTTTTTCGACCCTGCGGGAGGGGGGGACCCCATTCTT TATCAACATTTATTTTGATTTTTTGGTCACCNNGGAAAGTTTNAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_0455-scaled.jpeg,",40.5593540000,-75.4028623000,09/11/2025,"
Was seen together with a group. Seem attached to the flowers planted around the back of the campus.<\/p>\n",Lepidoptera,DNeasy (Qiagen),Abdomen,MiniPCR,1X TAE,SYBR Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_0580-scaled.jpeg,","",No,TNNNNNNNNNNNNNNNTTTGATCNGGACAGTCGGANNNCANNAAGAATANTNATTCNANNAGAACTATCTCNTCCTGGAN CATTATTCGNAATGATCNATTTATAACTGTANTGTTCCTTCANNNGCATTTGGANTAATCNTTTTATNANTATNCNAATT ATAAGTGGTGGTTTNGGAAACTGACTTGTTCNNCTTATAATGGGAGCACCTGATATAGCTTTCCCACGAATAAACANTAT AAGNTTTTGACTTTNACCAATATTATTTACATTATTAATTGCAAGATCAACAGCATCTTCAGGAACAGGAACAGGATGAA CTGTATACCCTCCTCTTTCGGCTNNAGAACNTCTTTCAGGACCGTCAGTAGATTTAACAATTTTTTNNTTCCACATTGTA GGTATTATATCAATTATATGAGCAATTAATTTTATTTCGACCATTATNAATATGCGAATCAAGGGAATAACAATATAAAA AACCCCCTTATTTTGTTGATCAGTACTAATTACTGTAATCTTNTTNNATTTTCTTTACCNTTACTATCCGTAGCAANNNC TCTATTAGTTANATACCGAAATCTAAATANNTTNNTTTTTGATACATCANGAGGANGNGATCCTATACTGTATCANATTT TATTNTGATTTTTNNTNNNAAAAAAAAAAAANAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_2765-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/image-4-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/image-5-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_2825-scaled.jpeg,",40.5593556617,-75.4025122548,09/11/2025,"
The carpenter bee has yellow fur and black body. The legs were stiff and pointy at the end. It was found in a bush with purple flowers. It was possibly pollinating the flower. It was aggressive when caught.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,1X TAE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_2825-1.jpeg,","
Lane 1: DNA Standard<\/p>\n
Lane 2: NHDM Arthropod #1 Wol +COI1 (Yellow Jacket)<\/p>\n
Lanes 3: NHDM Arthropod #2 Wol + COI1 (Bee)<\/p>\n
Lane 4: NHDM Wol Pos Wol + COI1<\/p>\n
Lane 5: NHDM Wol Neg Wol + COI1<\/p>\n
Lane 6: NHDM gDNA control Wol + COI1<\/p>\n
Lane 7: NHDM water control Wol + COI1<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_3707.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_3704.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_3706.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_3705.jpeg,",40.5589774639,-75.4026903916,09/11/2025,"
Found at a port-a potty. Seemed aggressive when caught. Summoned other yellow jackets<\/p>\n",Polistes,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3890-scaled.jpeg,","
$\"\"$ $\"\"$ <\/p>\n",Yes,ACTCTTTANANNNNNANTCGAAAGAATTAGGAGCGNNNCTTCTTTCTNN ATAANGATCATGTTATGATTGAATTAGTACNGCCCCTGCCTTAGGAATAATTTTTTTAATAGGTATNCCNTTTATAATTG GAGGTTTTTGAAAANGATNANTNCACTTAATACTCNAGNNCNCNGAAAAGGCTNTNCCTCTAGNANTNNTNNNANANTTT GAGTTGNACCACCCTCTTTATTTTTGCCTATTTTACAAAAGATAATANAAATAANATCAAGAACAGGAATAACTTGATAC CCCCCCTTGNNTACNNATCTTGGCNATAGTTCGCCTTCCNNNAANNNCGTTTTTGCTCTTCNTTTTGTTGNGNCTCATCC NTATANGACCTATTAAGNNATGGNNCACTATTTNATATACATATGATNACCGAATCATTAAATTTTTNANCTNTATATAC GTGAGCTTTTTTTTATACANCAAAGTTACTTTTATCATATTTACATTTNNTANCGCGAGCGATANCTATANNATTNNNTC TGANAGTANTANATACAANTTTTTTNATCCTAAGGAGAAGGNGAAACANATNNTTTNNAAAANATNTTGGTTTTTTGGCC ACATNNNNNTAAN,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_3423-1-scaled.jpeg,",40.559062964320475,-75.40310665242293,09/11/2025,"
Captured in sunlight by flowers and bees.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,1X TAE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_4705-2-scaled.jpeg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/20250911_154047-scaled.jpg,",,,,"
The spider that was caught resembles an orb weaver spider, but looking closer I am uncertain. It does have similar patterns to an orb weaver, but it could also be a different type of spider.<\/p>\n",Arachnida,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_3954.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_3953.jpeg,",40.5589625029,-75.4025319423,09/11/2025,"
Four large, membranous, and highly translucent wings. Held roof like or tent like over the body at rest. Venation is intricate and delicate (“lace-like”).<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,1X TAE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Image.png,","
\n
IAH AK DNA strand<\/li>\n
IAH AK Arth. #1 Wol + Col1<\/li>\n
IAH AK Arth. #2 Wol + Col1<\/li>\n
IAH AK Wol Pos +Col1<\/li>\n
IAH AK Wol Neg +Col1<\/li>\n
IAH AK gDNA Control Wol+ Col1<\/li>\n
IAH AK water control Wol+Col1<\/li>\n
IAH AK Empty<\/li>\n
KG LR DNA strand<\/li>\n
KG LR Arth. #1 Wol + Col1<\/li>\n
KG LR Arth. #2 Wol + Col1<\/li>\n
KG LR Wol Pos +Col1<\/li>\n
KG LR Wol Neg +Col1<\/li>\n
KG LR gDNA Control Wol+ Col1<\/li>\n
KG LR water control Wol+Col1<\/li>\n
KG LR Empty<\/li>\n<\/ol>\n",No,NNNNNNNNNNNNNNNNCNNGNNNNNTNNNGNNNTGTCGNCNNCTCANGNNGNGTGANNTTGNGTAAGACCCCCNACCTNC GCGNTCTCTTCCTATGANGCATCANTTANNGCTGGTGACTTTCAGGAAACTGCNANAGAAAAACTGGNNNAANGTGGGGA TGATGTCNCGTNATCATGTGCNNTGANNNTGGGCTACTCACATGCTTGAGGGCTNNNNTNGNTATGCCNAACNNCNGTAA ANTTTTCTCATNAAAGCCTTCTCCNTCTTATTGGCCCTGCAACTCAATTGCATAAAGGTGGAATNNCTANTAATCGTGGA TCACCACCCCACNGNGAATACCTTCTCNNGTCTTGNACACCCNGACCNNTACGCCATGGGTAAAGGTCCNNNNTNNNNGT ATTANCTTTTTANNGNTNTCATTTTCTTTTNCTCAACTATTTGANTCTNNAACAAATTTAATNTNNANTTAATAACTTTA TNTGANNGACCAGAAGTTCTTATATCAGNNGNTTTATTATTAATGTTNCTAGCAATNGTTGNACCGATTNCGTACTAAAN NACAGATNTANATGATTCAACTTCNTNNNNTANNCAACCATTATGAGTCGTTGATCCTTNATCAACNTTATTAAAATTTT TGTCTTTTTNCATCTNTTNNNNNNNNGNNANNNNNNNNAAGNTTA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_2049-scaled.jpg,",40.5472763,-75.41699179999999,09/11/2025,"
Assassin fly located in pollinator garden at PSU Lehigh Valley<\/p>\n",Asilidae,,,,,,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_3399-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_3392-2-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_3411-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_3403-scaled.jpg,",40.5589461266,-75.4026303216,09/11/2025,"
The spider was found under a leaf, balled up.<\/p>\n",,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_3566-1-scaled.jpeg,","",No,NNNNNNNNNNNNNNNNTGNNCTGCTATATTAGGAACAGCAATAAGAGTATTAATTCGAATTGAGTTAGGTCAACCGGGTA GATTTATAGGTGATGACCAATTATATAATGTAATTGTAACTGCTCATGCATTTGTAATAATTTTTTTTATGGTTATGCCA ATTTTAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCTCCTGATATAGCTTTTCCACGAATGAATAA TTTAAGATTTTGATTATTACCTCCTTCATTATTATTACTAATTACTTCATCAATAGTTGAAATAGGGGTTGGTGCTGGTT GAACAGTATATCCTCCTTTGGCTGGATTAGAAGGACATGCTGGTAGCTCAGTTGATTTTGCTATTTTCTCTTTACATTTA GCTGGGGCTTCTTCAATTATAGGGGCAATTAATTTTATTTCTACTATTATTAATATACGTTTTTATGGAATGACTATAGA AAAAGTTCCTTTATTTGTATGATCTGTATTAATTACAGCTGTTCTATTATTATTATCTTTACCAGTATTAGCTGGGGCTA TTACAATATTATTAACTGATCGAAATTTTAATACTTCTTTTTTTGATCCTTCTGGGGGTGGAGATCCTATTTTATTTCAN CATTTGTTTTGATTTTTTGGTCACCTNNAAANNTNNN,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/image1-scaled.jpeg,",40.5587570474,-75.4026874343,09/11/2025,"
Hand caught while predating on a Honeybee in a flowered mulch patch. Placed into plastic sandwich bag and frozen for 20 minutes after capture. Average sized Yellow Jacket with stripped and spotted thorax. Two bronze-colored wings attached to the abdomen. stinger not visible on specimen. Also processes two antennae protruding from the head and 6 legs attached to the abdomen.<\/p>\n",Vespula germanica,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/image0-1-1-scaled.jpeg,","
Lane 1:\u00a0 Arthropod 1 Wol + COI1 -> Crab Spider<\/p>\n
Lane 2: Arthropod 2 Wol + COI1 -> Yellow jacket<\/p>\n
Lane 3: Wol (+) Wol + COI1<\/p>\n
Lane 4: Wol (-) Wol +COI1<\/p>\n
Lane 5. gDNA control Wol +COI1<\/p>\n
Lane 6: Water Control Wol + COI1<\/p>\n
Lane 7: DNA Standard<\/p>\n
Taq Polymerase: GoTaq<\/p>\n
Master Mix: measured in Microliters<\/p>\n
\n
Water: 45.5<\/li>\n
GoTaq: 87.5<\/li>\n
F primer: 14<\/li>\n
P primer: 14\n
\n
23 Microliters of MM in each PCR tube<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n
Thermal Cycle<\/p>\n
\n
Denaturation: 98 degrees Celsius<\/li>\n
Annealing: 45-65 degrees Celsius<\/li>\n
Extension: 72 degrees Celsius\n
\n
Repeat roughly 30x = Billions<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n",No,TTTAATTAATAATGATCAAATTTATAATACTATTATTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTTATACCTT TTTTAGTTGGAGGATTTGGAAATTGATTAATCCCTTTAATATTAGGTGTTCCTGATATAGCATTTCCTCGAATAAATAAT ATAAGATTTTGATTATTACCTCCATCCTTATTTTTATTAATCTTAAGAAATTTTATTGGAACCGGAGTAGGGACAGGATG AACTCTGTACCCTCCTTTATCATCAATTGTAGGACATGACTCTCCCTCTGTAGACTTAGGAATTTTTTCTATCCATATTG CTGGAATTTCATCAATTATAGGTTCAATTAATTTTATTGTTACTATTTTAAATATACACACAAAAACACATTCACTAAAT TTTCTTCCTTTATTTTCATGATCAATTTTAATTACAGCAATTCTTCTCTTGTTATCTCTACCAGTTCTTGCAGGAGCAAT TACTATACTTCTTACAGACCGTAATTTAAATACATCTTTCTTTGATCCTGCAGGCGGAGGTGACCCAATTTTATACCAAC ATTTATTTTGATTTTTTGGTCACCTGGNAAGTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/20250911_102111-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/20250911_101933-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/20250911_102108-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/20250911_101940-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/20250911_101959-scaled.jpg,",40.5597317626,-75.4024337757,09/11/2025,"
Found in small leafy fern right to lion statue outside the main entrance of campus. Small spider of a greenish color with a yellow and black abdomen. Red eyes, superior leg pairs about double in length to inferior two leg pairs. Small brown hairs growing in density on distal side of legs and back of abdomen.<\/p>\n",Misumena,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/11/134692-scaled.jpg,","",No,NNNNNNNNNNNGNGNNNTGATCTGCTATAGTTGGGACTGCTATAAGAGTGTTGATTCGAATGGAGTTAGGAAATTCTGGA AGATTATTAGGAAATGATCATTTATATAATGTAATTGTTACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTTATACC AATTTTGATTGGTGGGTTTGGGAATTGATTAGTACCTTTAATATTGGGGGCACCTGATATATCTTTCCCACGTATAAATA ATCTATCTTTTTGATTACTTCCGCCTTCATTGTTTTTGTTATTTATATCATCTATGGTTGAAATAGGAGTAGGAGCAGGG TGAACTGTTTATCCTCCTTTAGCATCTAGTGTAGGGCATATAGGAAGATCTATGGATTTTGCTATTTTTTCTCTTCATTT AGCTGGAGCTTCATCTATTATAGGGGCTGTAAATTTTATTTCTACTATTATTAATATGCGAAGTCAAGGTATAAGAATGG AAAAGGTACCTTTATTTGTTTGATCTGTATTAATTACTGCTATTTTACTATTATTATCTCTTCCTGTTTTAGCTGGGGCT ATTACTATATTATTGACTGATCGTAATTTTAATACTTCATTTTTTGACCCTGCTGGAGGAGGGGATCCTATTTTATTTCA ACATTTATTTTGATTTTNNNNNNNN,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/tempImage3wZgus-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/tempImageU3LBcH-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/tempImageMz44yg-scaled.jpg,",40.5596894557,-75.4022962074,09/11/2025,"
Very calm throughout capturing process; green\/brown color with hints of yellow<\/p>\n",Orthoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/IMG_1854.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_1443-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_1445-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_1446-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_1448-scaled.jpeg,",40.5593413000,-75.4028326000,09/11/2025,"
Found it on the leaves of the bushes. This insect prefers to fly and jump from one location to another.<\/p>\n",Flatidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,1X TAE,SYBR Green,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/gel-.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/miniPCR-.jpg,","
Lane 1 is the DNA ladder;<\/p>\n
lane 2 is the target arthropod, siphanta acuta;<\/p>\n
lane 3 is my colleague’s arthropod;<\/p>\n
lane 4 is the positive wolbachia control;<\/p>\n
lane 5 is the negative wolbachia control;<\/p>\n
lane 6 is the wolbachia sampleDNA control (contain arthropod DNA);<\/p>\n
lane 7 is the water control;<\/p>\n
lane 8 is empty<\/p>\n
$\"\"$ <\/p>\n",No,TGGATTTGATCNGGACTAGTAGGAATAATAATAAGAATAATTATTCGAATAGAACTATCTCAACCAGGATCA ATAATCAAAAATGACCAAATTTATAACACAATTGTAACTTCACATGCATTTGTAATAATCTTTTTTATAGTTATACCAAT TATAATTGGTGGTTTTGGAAACTGGCTTGTTCCTCTTATAATTGGAGCACCAGATATAGCTTTCCCACGAATAAACAATA TAAGTTTTTGACTTTTACCAATATCAATTACATTATTAATTGCAAGATCAACAGCAGCTTCAGGAACAGGAACAGGATGA ACTGTATACCCACCTCTTTCGGCTCAAGAAGCACATTCAGGACCATCAGTAGACTTAACAATTTTTTCATTACACATTGC AGGTATTAGATCAATTATAGGAGCAATCAATTTTATTTCAACCATTATAAATATACGAATCAAAGGAATAACAATAGAAA AAACCCCCTTATTTTGTTGATCAGTACTAATTACAGCAATCCTTTTACTAGTTTCTTTACCAGTACTAGCCGGAGCAATC ACAATATTAATTATAGACCGAAATTTAAATACTTCATTTTTTGATCCATCAGGAGGAGGTGATCCTATTCTGTATCAACA CTTATTCTGATTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_2290-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_0666-scaled.jpeg,",40.5589856931,-75.4026181115,09/11/2025,"
Long skinny legs, brown, circular but small abdomen with pattern on the back<\/p>\n",Phalangium opilio,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system Bio-Rad,1X TAE,SYBR Safe,"","
\n
DNA standard<\/li>\n
Common Hentz jumping Spider<\/li>\n
Praying mantis<\/li>\n
Wolbachia positive Wol + COI1<\/li>\n
Wolbachia Negative Wol + COI1<\/li>\n
gDNA control Wol + COI1<\/li>\n
Water control Wol + COI1<\/li>\n
EMPTY<\/li>\n
DNA standard<\/li>\n
European Harvestman Spider<\/li>\n
Banded Tussock Moth Caterpillar<\/li>\n
Wolbachia positive Wol + COI1<\/li>\n
Wolbachia Negative Wol + COI1<\/li>\n
gDNA control Wol + COI1<\/li>\n
Water control Wol + COI1<\/li>\n
EMPTY<\/li>\n<\/ol>\n
<\/p>\n
DNA contamination between groups, skewing the results of the Harvestman spider identification, presenting it as a Northern Flatid Planthopper.<\/p>\n",No,CATTAACAGGTATAGTAGGAACTTCTTTAAGATTATTAATTCGAGCTGAATTAGGAAACCTGG TTTAATTGGAGATGATCAAATTTATAATACTATTTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCAA TTATAATTGGAGGATTTGGTAATTGATTAGTACCTTTAATATTAGGAGCTCCTGATATAGCATTTCCTCGAATAAATAAT ATAAGTTTTTGACTTTTACCCCCATCATTAACTTTATTAATTTCAAGAAGAATTGTAGAAAATGGAGCCGGAACAGGATG AACAGTTTATCCACCCCTTTCATCTAATATTGCCCATGGAGGTAGATCAGTAGATTTAGCTATTTTTTCCCTTCATTTGG CAGGTATTTCTTCAATTTTAGGAGCAATTAATTTTATTACTACAATTATTAATATACGATTAAATAACTTATCTTTTGAT CAAATACCTTTATTTGTTTGAGCTGTAGGAATTACAGCATTTCTATTATTACTTTCATTACCTGTATTAGCAGGAGCTAT TACAATATTACTAACTGATCGAAATTTAAATACATCATTTTTCGACCCTGCTGGAGGAGGAGATCCTATTCTTTACCAAC ATTTATTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_5124-scaled.jpeg,",40.5591424000,-75.4025487000,09/11/2025,"
Fuzzy greenish white color, black whisker like antennas<\/p>\n",Halysidota tessellaris,tweezers,Abdomen,Standard electrophoresis system,1X TAE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/Wolbachia.jpg,","
Lane1 DNA Standard<\/p>\n
Lane 2 Arthropod Spider<\/p>\n
Lane3 Athropod Preying Mantis<\/p>\n
Lane 4 WO1 POS WOL + COi1<\/p>\n
Lane 5 WOl neg WOI+ COI1<\/p>\n
Lane 6 gDNA COntrol WOI + COI1<\/p>\n
Lane 7 Water Control WOI + COI1<\/p>\n
Lane 8 Empty<\/p>\n
Lane 9 DNA Standard<\/p>\n
Lane 10 European Harvestman Spider<\/p>\n
Lane 11 Moth Caterpillar<\/p>\n
Lane 12 WO1 POS WOL + COi1<\/p>\n
Lane 13 5 WOl neg WOI+ COI1<\/p>\n
Lane 14 6 gDNA COntrol WOI + COI1<\/p>\n
Lane 15 Water Control WOI + COI1<\/p>\n
Lane 16 Empty<\/p>\n
<\/p><\/blockquote>\n",Yes,GGNATTTNAGCAGGTATAGTAGGAACTTCTTTAAGATTATTAATTCGAGCTGAATTAGGAAACCCTGGCT CTTTAATTGGAGATGATCAAATTTATAATACTATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTAT AGTTATACCAATTATAATTGGAGGATTTGGTAATTGATTAGTACCTTTAATATTAGGAGCTCCTGATATA GCATTTCCTCGAATAAATAATATAAGTTTTTGACTTTTACCCCCATCATTAACTTTATTAATTTCAAGAA GAATTGTAGAAAATGGAGCCGGAACAGGATGAACAGTTTATCCACCCCTTTCATCTAATATTGCCCATGG AGGTAGATCAGTAGATTTAGCTATTTTTTCCCTTCATTTGGCAGGTATTTCTTCAATTTTAGGAGCAATT AATTTTATTACTACAATTATTAATATACGATTAAATAACTTATCTTTTGATCAAATACCTTTATTTGTTT GAGCTGTAGGAATTACAGCATTTCTATTATTACTTTCATTACCTGTATTAGCAGGAGCTATTACAATATT ACTAACTGATCGAAATTTAAATACATCATTTTTCGACCCTGCTGGAGGAGGAGATCCTATTCTTTACCAA CATTTATTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/09/IMG_0668-scaled.jpeg,",40.5472763000,-75.4169918000,09/11/2025,"
Red, black, circular, small, spotted.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/10/tempImage9BW05e-scaled.jpg,","
Lane 9- LTHZ 01 – DNA standard<\/p>\n
Lane 10- LTHZ 01 – Arthropod 1 WOL + COI1 (Grasshopper)<\/p>\n
Lane 11- LTHZ 01 – Arthropod 2 WOL + COI2 (Ladybug)<\/p>\n
Lane 12- LTHZ 01 – WOL + (Positive)<\/p>\n
Lane 13 LTHZ 01- WOL – (Negative)<\/p>\n
Lane 14- LTHZ 01 – gDNA CNTRL<\/p>\n
Lane 15- LTHZ 01 – Water<\/p>\n
Lane 16- LTHZ 01 – Empty<\/p>\n",Yes,ANNNNNNNTTNNNNNNNNGNANNNGGGCAGGAATAGTAGGAACATCGTTNNNTATTTTAATTCGGTTAGAATTANNANNN NNAGGAAGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGT AATACCTATTATAATTGGGGGTTTTGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGAT TAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGA ACAGGATGAACTGTTTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTT ACATTTAGCAGGAATTTCCTCAATTTTAGGTGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAA TACTTGATAAAACTCCTTTATTTGTATGATCTGTTCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCA GGAGCAATTACTATACTATTAACTGACCGAAACTTAAATTCTTCTTTTTTTGACCCAACCGGTGGGGGAGACCCAATTTT ATACCAACATTTATTTTGATTTTTTGGTCACCTNGNAAGTTNAAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_7381-scaled.jpeg,",40.7933949000,-77.8600012000,03/31/2025,"
Found inside the house near a door.<\/p>\n",Harmonia axyridis,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Wolbachia-Gel-2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Arthropod-Gel.jpg,","
My results were in lane 3 for both gels. One gel image confirmed the arthropod had Wolbachia, and the other confirmed it was an arthropod.<\/p>\n",Yes,AGTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAGAGGAAGATTAATTGGAAACGACCAAATTTATA ATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTTTGGAAATTGA TTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGC TTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTTTACCCTCCTCTTTCTTCTA ATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCA GTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGATAAAACTCCTTTATTTGTATGATCTGT TCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTATTAACTGACCGAAACT TAAATTCTTCTTTTTTT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/NP2.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/DSC10295-scaled.jpg,",43.2543597000,-112.3260882000,06/05/2025,"
Found dead.<\/p>\n",Monochamus scutellatus,Edwards Buffer,Abdomen,Standard electrophoresis system,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/Screenshot-2025-07-16-195147.png,","",No,GGAATAGTAGGAACATCTTTAAGATTACTAATCCGTTCAGAACTAGGAATACCTGGATCTTTAATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCTCATGCATTTATCATAATTTTTTTTATAGTTATACCAATTATAATTGGTGGATTTGGAAATTGGTTAGTTCCTCTTATGTTAGGAGCTCCTGATATAGCTTTTCCTCGAATAAATAATATAAGATTTTGACTTTTACCTCCTTCTCTAACTCTTTTAATTATAAGAAGAATTGTAGAAAATGGAGCAGGAACTGGATGAACAGTATATCCCCCTCTAGCAGCAAATGTTGCTCATAGAGGTTCATCAGTTGATTTAGCAATTTTTAGTCTTCATCTAGCTGGAATTTCTTCTATTCTAGGTGCAGTAAATTTTATTACAACTGTAATTAATATACGACCTAAAGGAATAAATTTAGATCGTTTACCTTTATTTGTTTGAGCAGTAAAAATTACTGCAATTCTTCTTCTTCTTTCTTTACCAGTATTAGCTGGGGCAATTACTATACTTTTAACAGACCGAAATCTAAATACATCATTCTTTGATCCAGCAGGAGGAGGAGATCCAATTTTATATCAACATTTATTTTGATTTTTTGGTCAC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/DSC10294-scaled.jpg,",43.2543597000,-112.3260882000,06/05/2025,"",Eleodes armata,Edwards Buffer,Abdomen,Standard electrophoresis system,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/Screenshot-2025-07-16-194005-1.png,","",No,ACTATTAATCCGAGCAGAATTAGGAAATCCCGGTTCACTAATTGGAGACGATCAAATTTATAATGTAATTGTAACGGCCCACGCTTTTATCATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGATTTGGTAACTGGTTAGTCCCATTAATATTAGGAGCTCCTGATATAGCATTCCCACGAATAAATAATATAAGATTTTGACTTTTACCCCCCTCTTTAACACTTTTGCTAATAAGAAGAATCGTAGAAAATGGAGCAGGGACAGGATGAACAGTGTATCCCCCACTATCATCAAATATCGCTCATAGAGGATCTTCCGTAGACTTAGCCATCTTTAGTTTACATCTAGCAGGAATCTCTTCCATTTTAGGAGCAGTTAATTTTATTACTACAGTAATTAATATACGACCAAAAGGAATAACATTTGATCGAATACCCCTATTCGTTTGAGCAGTTGTTATTACCGCTGTATTATTATTACTATCACTTCCAGTTCTAGCAGGAGCAATTACAATACTATTAACAGATCGAAACATTAATACATCCTTTTTTGACCCCGCTGGTGGTGGAGATCCTATTTTATATCAACACCTGTTTTGATTTTTTGGTCACCC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/DSC10293-scaled.jpg,",43.2543597000,-112.3260882000,06/05/2025,"
Caught alive.<\/p>\n",Gryllus veletis,Edwards Buffer,Abdomen,Standard electrophoresis system,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/Screenshot-2025-07-16-194005.png,","",No,AGTAGGCACATCATTAAGTATTTTAATTCGAACAGAACTAGGACAACCAGGTTATTTAATTGGAGACGATCAAACTTATAATGTTATCGTAACTGCACATGCATTTATCATAATTTTCTTTATAGTTATACCTATTATAATCGGAGGATTTGGAAATTGATTAGTACCACTAATATTAGGAGCTCCAGATATAGCATTCCCACGAATAAATAACATAAGATTTTGACTCTTACCCCCATCATTAACCCTTTTATTAACCAGAAGAATAGTTGAAAATGGTGCAGGAACAGGATGAACAGTCTATCCACCTCTATCAACAGGAATTGCTCACGCTGGAGCATCTGTTGATTTAGCTATCTTCTCACTACATTTAGCAGGAATTTCCTCAATTCTAGGAGCTGTAAATTTTATTACTACTATAATTAATATACGAGCACCAGGAATATCACTAGACCAAACACCATTATTTGTTTGAGCAGTAGGAATTACAGCTCTTCTACTATTATTATCATTACCAGTTTTAGCTGGTGCTATTACAATACTACTTACAGATCGAAATTTAAATACATCATTTTTTGATCCTGCAGGAGGAGGAGATCCAATCTTATATCAACATTTATTTTGATTTTTTGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/DSC10292-scaled.jpg,",43.2543597000,-112.3260882000,06/04/2025,"
Found living.<\/p>\n",Trochosa ruricola,Edwards Buffer,Whole arthropod,Standard electrophoresis system,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/Screenshot-2025-07-16-193703.png,","",No,TGCTATAAGAGTGTTAATTCGTTTAGAATTAGGAAATTCTGGAAGTTTATTAGGAGATGATCATTTATATAATGTTATAGTTACTGCTCATGCTTTTGTGATGATTTTTTTTATAGTAATGCCAATTCTTATTGGAGGTTTTGGTAATTGATTAATTCCTTTAATGTTAGGTGCTCCTGATATATCATTTCCTCAAATGAATAATCTTTCTTTTTGGTTATTGCCCCCTTCTTTATTTTTATTAACTATGTCTTCTATAGTGGAAATAGGGGTAGGAGCTGGTTGAACAGTTTATCCTCCTTTAGCATCTAGAATAGGTCATATAGGGAGTTCTATAGATTTTGCTATTTTTTCTCTTCATTTAGCTGGTGCTTCTTCTATTATAGGGGCAGTAAATTTTATTTCTACTATTATTAATATACGAATATTAGGTATATCTATAGATAAGGTACCTTTATTTGTTTGGTCAGTTTTGATTACGGCTGTTTTATTATTATTATCTTTACCTGTTTTANCAGGTGCTATTACTATGTTATTAACTGATCGAAATTTTAATACTTCTTTTTTTGACCCTGCAGGGGGAGGAGATCCTATTTTATTTCAACATTTGTTTTGATTTTTTGGTCACCCTGAAGTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/DSC10275-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/DSC10276-scaled.jpg,",43.25435969999999,-112.3260882,06/04/2025,"
Caught live straight from the beehive.<\/p>\n",Apis mellifera,Edwards Buffer,Abdomen,Standard electrophoresis system,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/Screenshot-2025-07-16-193340.png,","",No,TTCTAGCTTTATGATCTGGAATACTAGGATCATCAATGAGACTTATTATTCGAATAGAATTAAGATCCCCAGGATCATGAATTAGCAATGATCAAATTTATAATACAATTGTTACTAGTCATGCATTCCTAATAATTTTTTTTATAGTTATACCATTTTTAATTGGAGGATTTGGAAATTGGCTTATTCCTTTAATACTAGGATCACCTGATATAGCATTCCCCCGAATAAATAATATTAGATTTTGATTACTTCCTCCCTCATTATTTATACTTTTATTAAGAAATTTATTTTATCCAAGACCAGGAACTGGATGAACAGTATATCCACCATTATCAGCATATTTATATCATTCTTCACCTTCAGTAGATTTTGCAATTTTTTCTCTTCATATATCAGGAATTTCCTCAATTATAGGATCATTAAACTTAATAGTTACAATTATAATAATAAAAAATTTTTCTATAAATTATGACCAAATTTCATTATTTCCATGATCAGTTTTTATTACAGCAATTTTATTAATTATATCATTACCTGTATTAGCTGGAGCAATTACTATACTATTATTTGATCGAAATTTTAATACATCATTTTTCGATCCTATAGGAGGTGGAGATCCAATTCTTTATCAACATTTATTTTGATTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/DSC10291-scaled.jpg,",43.2543597000,-112.3260882000,,"
Found dead.<\/p>\n",Cynomyopsis cadaverina,Edwards Buffer,,Standard electrophoresis system,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/Screenshot-2025-07-16-192829.png,","",No,GATCGGAACTTCATTAAGAATTTTAATTCGAGCCGAACTAGGACACCCTGGAGCATTAATTGGTGATGACCAAATCTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGATTTGGTAATTGATTAGTCCCTTTAATGTTAGGAGCCCCAGATATAGCTTTCCCTCGGATAAACAATATAAGTTTCTGACTTTTACCTCCTGCATTAACTTTACTATTAGTAAGTAGTATAGTGGAAAACGGAGCTGGAACAGGATGAACTGTTTACCCACCTTTATCATCTAATATCGCTCATGGAGGAGCTTCTGTTGATTTAGCTATTTTTTCTTTACACTTAGCAGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACAACAGTTATTAATATACGATCAACAGGAATTACTTTTGACCGAATACCACTATTCGTTTGATCAGTAGTAATTACAGCTTTATTACTTTTACTATCTTTACCTGTTCTAGCTGGTGCTATTACAATATTATTAACAGACCGAAACCTTAATACTTCATTCTTCGACCCAGCAGGAGGAGGAGACCCAATTCTATACCAACATTTATTTTGATTTTTTGGTCACCTGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/DSC10290-scaled.jpg,",43.2542838317,-112.3261739605,06/04/2025,"
Found dead.<\/p>\n",Hecatera dysodea,Edwards Buffer,Abdomen,Standard electrophoresis system,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/Screenshot-2025-07-16-192300.png,","",No,GGATTTGAGCAGGAATAGTAGGAACTTCATTAAGATTATTAATTCGAGCTGAATTAGGAACCCCAGGATCTTTAATTGGAGATGATCAAATTTATAATACTATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATGGTTATACCTATTATAATTGGAGGATTTGGTAATTGACTTGTCCCCTTAATATTAGGAGCCCCAGATATAGCATTTCCTCGTATAAATAATATAAGTTTCTGATTTCTTCCCCCTTCTTTAACCTTATTAATTTCAAGAAGAATTGTAGAAAATGGAGCAGGAACTGGATGAACAGTGTACCCCCCACTTTCATCTAATATTGCTCATGGAGGAAGATCAGTAGATTTAGCTATTTTTTCTCTTCATTTAGCTGGTATTTCTTCTATTCTTGGAGCTATTAATTTTATTACTACAATTATTAATATACGATTAAATAATTTATCTTTTGATCAAATACCTCTATTTATTTGAGCCGTAGGAATTACTGCATTTTTATTATTATTATCTTTACCTGTATTAGCTGGAGCTATTACTATACTTTTAACAGACCGAAATTTAAATACATCTTTTTTTGACCCTGCTGGAGGAGGAGATCCAATTTTATATCAACATTTATTTTGATTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/DSC10289-scaled.jpg,",43.254123916434594,-112.32631806034944,06/03/2025,"
Caught alive in shade.<\/p>\n",Tetramorium caespitum,Edwards Buffer,Whole arthropod,Standard electrophoresis system,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/Screenshot-2025-07-16-191516.png,","",No,TTGCTATTTGAGCAGGATAATCGGATCTTCAATAAGTATAATTATCCGACTTGAATTAAGTTCCTGTGGTTCATTAATTAATGACGACCAAGTATATAATTCCTTAGTAACAAGACATGCCCTTGTTATAATTTTCTTTATAGTAATGCCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCCATTAATACTAGGAACTCCCGATATAGCCTACCCCCGTATAAATAACATAAGATTTTGACTTCTACCACCCTCTATTAGATTATTATTGCTTAGAAGATTTATTAATCCAGGGGTAGGAACAGGATGAACTATCTATCCCCCATTAGCATCTAATATCTTTCATAGAGGCCCATCAATTGATCTATCAATTTTTTCTCTTCATATTGCAGGAATATCTTCTATTATAGGTGCAATTAATTTTATCGCTACTATTATAAACATACATCATAAAAAATTAACCTTAGATAAAATCCCCCTGTTAGTTTGATCTATTTTAATTACAGCAATTCTTCTCCTCCTATCTCTTCCCGTATTAGCAGGAGCTATTACTATACTCTTAACAGATCGTAATTTAAATACCTCATTTTTTGATCCATCAGGTGGGGGGGACCCAATTCTATATCAACATTTATTCTGATTTTTTG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/DSC10273-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/DSC10274-scaled.jpg,",43.2542713532,-112.3260845822,06/03/2025,"
Caught dead.<\/p>\n",Apis mellifera,Edwards Buffer,Abdomen,Standard electrophoresis system,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/Screenshot-2025-07-16-185917.png,","",No,TCTAGCTTTATGATCTGGAATACTAGGATCATCAATGAGACTTATTATTCGAATAGAATTAAGATCCCCAGGATCATGAATTAGCAATGATCAAATTTATAATACAATTGTTACTAGTCATGCATTCCTAATAATTTTTTTTATAGTTATACCATTTTTAATTGGAGGATTTGGAAATTGGCTTATTCCTTTAATACTAGGATCACCTGATATAGCATTCCCCCGAATAAATAATATTAGATTTTGATTACTTCCTCCCTCATTATTTATACTTTTATTAAGAAATTTATTTTATCCAAGACCAGGAACTGGATGAACAGTATATCCACCATTATCAGCATATTTATATCATTCTTCACCTTCAGTAGATTTTGCAATTTTTTCTCTTCATATATCAGGAATTTCCTCAATTATAGGATCATTAAACTTAATAGTTACAATTATAATAATAAAAAATTTTTCTATAAATTATGACCAAATTTCATTATTTCCATGATCAGTTTTTATTACAGCAATTTTATTAATTATATCATTACCTGTATTAGCTGGAGCAATTACTATACTATTATTTGATCGAAATTTTAATACATCATTTTTCGATCCTATAGGAGGTGGAGATCCAATTCTTTATCAACATTTATTTTGATTTTTTGGTC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/C8.jpeg,",41.6747200000,-77.0652300000,10/07/2024,"
Location – Under a tree on the river bank<\/p>\n
Notes – Flying around spiderwebs, slow flier<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/C8.png,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/D9.jpeg,",41.6749900000,-77.0633900000,10/29/2024,"
Location – Under fallen leaves on riverside<\/p>\n
Notes – Capable of quick (~3 inch) evasive jumps<\/p>\n",Cicadellidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/D9.png,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/B9.jpeg,",41.6739800000,-77.0627100000,10/29/2024,"
Location – In riverside reeds, climbing on a blade of grass<\/p>\n
Notes – Appeared to possess wings, but did not attempt to fly away<\/p>\n",Lonchoptera bifurcata,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/B9.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/A9.jpeg,",41.6749900000,-77.0633800000,10/29/2024,"
Location – In sandy gravel riverside<\/p>\n
Notes – Needed to be dug up with a trowel<\/p>\n",Entomobrya nivalis,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/A9.png,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/DSC10288-scaled.jpg,",43.2539821774,-112.3253416484,06/03/2025,"
Found dead.<\/p>\n",Polistes dominula,Edwards Buffer,Abdomen,Standard electrophoresis system,1X TAE,GelGreen,"","",No,TTGGTACATCTTTAAGATTAATAATTCGATTAGAATTAGGAACACCATCTTCAATTATTAATAATGATCAATTTTATAATTCAATTATTACAGCCCATGCTTTAGTAATAATTTTTTTTATAGTTATACCATTTATAATTGGAGGTTTTGGAAATTGATTAATTCCTATAATACTTGGAGCCCCTGATATAGCTTTCCCACGAATAAATAATATAAGATTTTGACTTTTACCACCCTCATTATTTTTGCTTATTATAAGAAACATAATAAAAATAGGAGTAGGAACAGGATGAACTTTATACCCACCTTTATCTTCAAATATTGGACATAATTCACCTTCTGTTGATTTATCAATTTTTTCTCTTCATATTGCTGGAATCTCATCCATTATAGGAGCTATTAATTTTATTGTAACAATTTTAAATATACATATTAAAACCCATTCATTAAATTTTTTACCTTTATTTACATGATCTGTTTTAATTACAGCAATTTTACTTTTATTATCTTTACCTGTCTTAGCAGGAGCAATCACTATACTATTAACTGATCGAAATTTAAATACATCTTTTTTTGATCCTACAGGAGGAGGTGATCCAATTTTATTTCAACATCTATTTTGATTTTTTGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21C2.1Foto-1.jpg,",46.548307946,9.9008811508,10/02/2024,"",Baetidae,Instagene Matrix,Abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21C2.1Gel-1.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/C2.1_Co1-F-3.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21A2.1Foto.jpg,",46.548307946,9.9008811508,10/02/2024,"",Diptera,Instagene Matrix,Abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21A6Gel-1.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/A2.1_Co1-F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21A6Foto.jpg,",46.548307946,9.9008811508,10/02/2024,"",Baetidae,Instagene Matrix,Abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21A6Gel.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/A6_Co1-F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21A2.3Foto.jpg,",46.548307946,9.9008811508,10/02/2024,"",Baetidae,Instagene Matrix,,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21A2.3Gel-1.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/A2.3_Co1-F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21C6_Foto.jpg,",46.5483079460,9.9008811508,10/02/2024,"",Medetera jacula,Instagene Matrix,Abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21C6_Gel.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/C6_Co1-F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21C3Foto.jpg,",46.548307946,9.9008811508,10/02/2024,"",Plecoptera,Instagene Matrix,Whole arthropod,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21C3Gel.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/C3_Co1-F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21C_2.2.jpg,",46.548307946,9.9008811508,10/02/2024,"",Baetidae,Instagene Matrix,Abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Gel_C2.2.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/C2.2_Co1-F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21C_11.jpg,",46.548307946,9.9008811508,10/02/2024,"",Formica,Instagene Matrix,Abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Gel_C11.jpg,","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/C8_Co1-F-1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21C_81.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21C_82.jpg,",46.548307946,9.9008811508,10/02/2024,"",Philaenus,Instagene Matrix,Abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Gel_C8.jpg,","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/C8_Co1-F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21A_12.jpg,",46.548307946,9.9008811508,10/02/2024,"",Ichneumonidae,Instagene Matrix,body without wings,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Gel_A12.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/A12_Co1-F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21A_11.jpg,",46.548307946,9.9008811508,10/02/2024,"",Formica,Instagene Matrix,Abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Gel_A11.jpg,","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/A11_Co1-F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21A_10-1.jpg,",46.548307946,9.9008811508,10/02/2024,"",Pollenia,Instagene Matrix,Partial abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Gel_A10.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/A10_Co1-F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/21A_7.jpg,",46.5482489175,9.9006236588,10/02/2024,"",Chorthippus brunneus,Instagene Matrix,Partial abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Gel_A7.jpg,","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/A7_Co1-F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/877896ED-E1FD-47AB-920E-480E0CAB0F75_1_105_c.jpeg,",40.6200368293,-74.5679684559,06/03/2025,"
The insect was found crawling up a tree in the woods behind the Pingry School. The insect had a\u00a0black-colored body, three segments, six legs, and two antennae<\/p>\n",Camponotus pennsylvanicus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/046B2714-98DF-4DBD-8038-6C8EA56E25D5_1_201_a-1.jpeg,","
\n
Cell lysis and Precipitation<\/li>\n
DNA purification<\/li>\n
DNA elution<\/li>\n
PCR<\/li>\n
Gel Electrophoresis<\/li>\n<\/ol>\n",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/MD16_MC_A-Premixed-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/IMG_4460.jpg,",40.6204014339,-74.5676934356,05/30/2025,"
I found the bug next to the birdfeeder at the Pingry School. It was in a patch of dirt, and there were numerous specimens of the same species. The arthropod has a smooth, oval body and is black. It is around 2 cm in length and 0.5 cm in width.<\/p>\n",Camponotus pennsylvanicus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screenshot-2025-06-06-at-10.33.17-AM.png,","
The first step was DNA extraction, then DNA purification, and then DNA elution. After that, I started PCR and created my gel. During my gel loading, I accidentally put my Wolbachia sample on the top row. It is the last well in the top row and is showing DNA at that well, signifying that there is Wolbachia present in my arthropod sample. After this, I did PCR purification and finally sequencing.<\/p>\n",Yes,NNNNNNNNNNNNNNNNNNNNNNNNGTTNANATTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTGGGATCTCCTGN NNNACTAATTCTTAATGATCAAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATAC CTTTTATAATTGGGGGATTTGGTAATTGATTAATTCCACTTATACTAGGATCTCCTGATATAGCTTACCCTCGTTTAAAT AACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGG TTGAACTATCTACCCCCCTCTATCATCAAATACCTTCCNNNNNGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATA TTGCTGGNATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACNATTATAAATATACATAATTCCAATATTTCCCNN NATAAAATTCCATTATTATNATGATCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGGGC CTATTNNNTACTACTAACAGACCGAAAATCTTAATACTTCATTTTTTCGATCCCTCGGGGAGGANGAGATCCTATTTTTA TACCAACATTTATTTTGAATTTTTTGGGTCACCCTTGAAAGTTTAAN,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/MD11_IK_A-Premixed.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screenshot-2025-06-06-at-9.11.09 AM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screenshot-2025-06-06-at-9.12.18 AM.png,",40.6198806344,-74.5694667271,08/05/2025,"
It was found, perhaps injured, not flying, and not moving much on a gravel hiking trail in the woods. It was just standing there in the middle of the path. Thus, it was easy to pick up off the ground and capture.<\/p>\n",Spilomena beata,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/AnnotatedGel03.png,","
Extract the DNA using the DNeasy (Qiagen) blood and tissue kit, and then amplify the Arthropod COI gene and the potential Wolbachia gene using PCR. After DNA was amplified, it was stained with SYBR Safe and run through a gel. Then, samples that had bands for COI or Wolbachia were sequenced using the QIAquick PCR Purification kit and sent out for sequencing.<\/p>\n",Yes,TGGNTTTGGTATTNCAGGGANACCTCNATCACAGNNANTNCTGTCGCCNCNCCNGGANANTNTNTTATGATAAATCCTGA ACTGCACTTATGANCNCTNNTCAAACTTTCTGATATCNTGATNCAATGGCTGAAGGAAGGTGTNCNGATNNANNCCATGA GTTCAAAGAAGGCCAAGATGAGCTGNCGGAGCAGGATGAAATGTTCANAGGCCGGACTGCAGTGATTGCTGATCAAGTGA TANTTGGCAATGCCTCTTTGCGGCTGANNAACTTGTAACTCACAGATGCTGGCACCTACNAATGTTATATCATCACTTCA AGANGCA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/MD01_JZ_A-Premixed.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screenshot-2025-06-05-at-6.56.02-PM.png,",40.6150697796,-74.5677819357,06/03/2025,"
Big, flat black Carpenter Ant with six legs and a bulbous abdomen covered with fine hair.\u00a0 Found on the ground in the woods, along with a few other carpenter ants that surrounded it.<\/p>\n",Camponotus pennsylvanicus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,1X TAE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screenshot-2025-06-05-at-7.06.48-PM.png,","
Extraced DNA using the DNeasy extraction kit, and then used the DNA extracted to amplify the Wolbachia and arthropod CO1 genes with PCR. After PCR, took 10 microliters of the PCR product and 4 microliters of loading dye and put into 1.5 mL tubes. Then made 2% agarose gel with TAE buffer with double wells (8 wells, two for 16 wells in total). Then loaded 100 kb ladder (very left). the wells on the top represent arthropod amplified samples and the wells on the bottom represent Wolbachia amplified wells.\u00a0 Annotations in Image, but if not accessible\/difficult to see, the order goes (for both top and bottom): Ladder, Bug Sample, Positive Arthropod Control, Negative Arthropod Control, Positive DNA control, Negative DNA control.<\/p>\n",Yes,TTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTAGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCAAT ACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTT AATTCCACTTATACTAGGATCTCCTGATATAGCTTACCCTCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATCGA TCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGAACTATCTACCCCCCTCTATCATCAAAT ACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAAT CAATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTAGTATGATCTATTC TTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGCGCTATTACAATACTACTAACAGACCGAAATCTT AATACTTCATTTTTCGATCCCTCGGGAGGAGGAGATCCTATTTTATA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/MD09_AL_A3-Premixed.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screenshot-2025-06-05-at-4.38.29 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screenshot-2025-06-05-at-4.38.38 PM.png,",40.6200000000,-74.5690000000,06/03/2025,"
A black insect with a brown thorax. It has six legs, mandibles, and antennas<\/p>\n",Camponotus,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screenshot-2025-06-06-at-9.13.51 AM.png,","
DNA was extracted using the DNeasy extraction kit. Then, PCR was performed to amplify DNA in order to test for Wolbachia and arthropod DNA in gel electrophoresis. We used 2% gel using 1X TAE buffer and agarose. In the gel, the top wells were used to test for arthropod DNA while the bottom wells were used to test for the presence or absence of Wolbachia. 10 microliters of sample and 4 microliters of dye were loaded into the gel. The first wells on both the top and bottom were the ladder, then the bug sample, positive and negative arthropod controls, and a negative DNA control.<\/p>\n",Yes,TTTATTAATGAAGGATCTGGAACTGGTTGGACTGTCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTAGTATGGTCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGAGCTATTACAATACTACTAACAGACCGAAATCTTAATACTTCATTTTTCGACCCTTCGGGAGGGGGAGATCCTATTTTATACCAACATTTATTTTGATTTTTTGGTCACCC,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/MD03_FR_A-Premixed.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screenshot-2025-06-05-at-1.03.16 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screenshot-2025-06-05-at-1.03.31 PM.png,",40.6867556000,-74.5580454000,05/27/2025,"
Arthropod lives amongst dry leaves and sticks, crawls around. Arthropod has 4 wings, 2 long and 2 short, fur on the tail, lighter lines going across his black body.<\/p>\n",Camponotus pennsylvanicus,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screenshot-2025-06-05-at-1.08.58 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screenshot-2025-06-05-at-1.09.26 PM.png,","
DNA Extraction using DNeasy (Qiagen) Kit<\/p>\n
1. Cell lysis and DNA precipitation<\/p>\n
2. DNA purification using column chromatography<\/p>\n
3. DNA Elution using AE buffer<\/p>\n
Checking DNA Extraction With Nanodrop<\/p>\n
– Arthropod DNA concentration: 50.8 ng\/uL,\u00a0230 nm<\/p>\n
PCR using Taq Master Mix<\/p>\n",Yes,CTCCTGATTCACTAATTCTTAATGATCAAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATA GTTATACCTTTTATAATTGGGGGATTTG,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/MD10_TL_A-Premixed.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/IMG_4169-scaled.jpeg,",40.6202911163,-74.5687771168,05/30/2025,"
The bug was found in the forest part of Pingry School, under a big rock. The bug moved very slowly when the rock was lifted. It had a white body color, 2 eyes, and was smaller than a grain of rice.<\/p>\n",Melanoliarus placitus,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screenshot-2025-06-06-at-10.43.42 AM.png,","
First, DNA was extracted from the bug, then purified the DNA, and then eluted the DNA. After starting PCR. After PCR was created and loaded the gel was loaded. After we did PCR purification and then sequencing.<\/p>\n",No,NNNNNNNNGNNNNNNNNNNNNNNNNNNNNNGACTTATNNGNNNNATNNNANNAATAATTATCCGAATAGAATTAACTCAT CCNGGCTCAATCATCAACAACGATCAAATCCACAACATAATANTAACCTCACATGCTTTCATTATGATCTTCTTTATAGT NATACCAATTATAATTGGAGGATTCGGAAACTGATTAGTCCCAATTATAATTGGAGCTCCNGACATAGCATTCCCCCTAA TAAACAACATAAGATTTTGACTCCTACCACCTTCTTTAATATTAATAATTTCCAGAAGATTAACAGGATCTGGAGCAGGG ANNGGGNGAACAGTATATCCACCTCTGGCTAGATTAACAGCCCATTCAGGCCCATCTGTAGAGATNACAATTTTTTCTCT CCCTATTGNANGTGTTAAATCAATCCTAGGAGNAATCTACTTTATCTCNACTATTTTAAATATACGATCANAAGAAATAA CACCNGAAAAAACACCCCTATTTTGTTGATAAGTTCTAATTACAGCNATTTTNCTATTAATTTCNCTNCCCNTGTNNTAG GAGCNATTANTGTATTACTTATNGACCGAAATTTTCATANAACATTCTTTGATCCTACCTGAGGAGGAGATCNACTNNTA NTTANCAATTATTCTGATGATTTGNTNNACNGNNTNNAANTAGN,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/MD07_LS_A-Premixed-1-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screen-Shot-2025-06-05-at-10.44.24-AM.png,",40.6201738982,-74.5677416680,05/30/2025,"
The bug is small, green, with multiple long-legs. It has a leaf-like structure and moves by hopping and jumping. The bug was found beneath a rock in a forested area.<\/p>\n",Hemiptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screen-Shot-2025-06-05-at-11.11.58-AM.png,","
DNA was extracted from the bug, and then the DNA was purified before it went through elution. The extracted DNA was then amplified through PCR, which was then analyzed by gel electrophoresis. The amplified DNA was then purified and was submitted for sequencing<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/MD06_NS_A-Premixed.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screenshot-2025-06-05-at-11.17.57 AM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screenshot-2025-06-05-at-11.18.32 AM.png,",40.6200904794,-74.5685562056,05/30/2025,"
The bug was hopping from grass blade to blade, somewhat camouflaging itself with the greenery around it. It looked a little lighter than the green grass and more transparent, so if you looked closely, you could see it. It barely flew much, mainly just hopping and using it’s wings to glide or gain a bit more air. It primarily stayed low and still, only hopping away when it felt it was trying to be grabbed.<\/p>\n",Chrysopidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screenshot-2025-06-05-at-11.06.29 AM.png,","
Extract the DNA using the DNeasy (Qiagen) blood and tissue kit, and then amplify the Arthropod COI gene and the potential Wolbachia gene using PCR. After DNA was amplified, it was stained with SYBR Safe and run through a gel. Then, samples that had bands for COI or Wolbachia were purified using the QIAquick PCR Purification kit and sent out for sequencing.<\/p>\n",No,GGGTNTAATACTTANAATATTGATTCNAGTAGAATTAGCACAACCTGGACCTTTACTTAATAATGATCAAGTATACAATG TAATTGTGACTTCTCATGCATTTATCATGATTTTTTTTATAGTTATACCAATTATAATCGGTGGTTTTGGGAATTGATTA TTACCATTAATAATTGGTGCACCAGATATAGCATTTCCACGAATAAATAATATAAGTTTTTGGTTATTACCTCCTTCCCT AACATTGCTATTAATTAGTTCTATAGNANNNATAGGTGCTGGNACNNGCTGAACAGNATACCCCCCACTATCNTCTAATA TTGCTCACTCTGGTCCAAGAGTGGATTTAGCTATTTTCTCGCTTCATTTAGCAGGAATCTCATCTATTTTGGGGGCAGTA AACTTCATTACAACAGNAATTAATATGCGTAGAATTGGGATAAAATTANACCGGACTCCTTTATTCGTCTGATCTGTTTT AATTACAGCAATTCTTTTACTTCTATCTCTACCAGTTTTAGCAGGAGCTATTACTATATTATTAACTGATCGTAATATTA ATACCTCCTTTTTTGATCCGTCCGGGGGTGGNGACCCTATTTTATACCAACATCTATTTTGATTTTTTG,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/MD18_JZ_A-Premixed.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screenshot-2025-06-05-at-10.49.16 AM.png,",40.6200000000,-74.5690000000,06/27/2025,"
A black bug with a reddish orange color on its back. It had 2 black lines going down vertically from its head to the bottom of the insect. It had a spiky head and 6 legs.<\/p>\n",Harmonia axyridis,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screenshot-2025-06-06-at-9.25.28 AM.png,","
Extracted DNA using the DNeasy extraction kit. Then, created a 2% agarose gel using 1X TAE buffer and agarose. 2 rows of wells were made, the top wells were to test for Arthropod DNA, the bottom wells were to test for Wolbachia DNA. PCR was completed on the samples to get them ready for gel electrophoresis. The first column was for the ladder, the second was my bug sample, the third and fourth were positive and negative arthropod controls, and the last column was a negative DNA control.<\/p>\n",No,GAGATGGTGCTNNAAGGNAACTTGAGTTTANCTGTAATATTTCGCTTGTATTTACAAGANTAATGCNTTCATGTGAATTG AGAAANTACTATGTTGTTATTACAATGGTTTTGTTTTGACTTTTTTTCTAACAAAACTATTTCATTGCATCTTACTAATC ATTTAAAAACCAGAAGAAAATGCATATTTGATTAAAATAGATCTCTTAANTCTAGTTCTCTTTTATGTATCAGGACTTGT GGGTTTTATTTTTANAAGGAAACCTTAATCACTGAAAGCAAAGGTGAAACCATCCTCCACNGAGGCTTNTCAGNAGGTGG GCGGACCATCCTGCAAAACGACCACTCACTACTGGCCAGCANCTGGGCTTCTGCNGAACTTCATGCCCCACCAGGTAAAG CAGGCCTCCNAAAGTCCAGTTTGNATGTTTTGGACTTTG,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/MD19_FR_A-Premixed.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/IMG_3070-2-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/IMG_3075-scaled.jpg,",40.6203272000,-74.5696351000,05/25/2025,"
A gray Roly-Poly speckled with brown on its dorsal side, with a yellow-tinted pouch on its ventral side, which was filled with eggs. The arthropod had 14 legs and two antennae and found under a rock on the trail behind the Pingry School and easy to pick up.<\/p>\n",Armadillidae,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,1X TAE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Screenshot-2025-06-06-at-8.49.04-AM.png,","
Extract DNA using DNeasy extraction kit, and then amplify both Wolbachia and arthropod CO1 gene with PCR. After PCR, took 10 microliters of the PCR product and 4 microliters of loading dye and separated into 1.5 mL tubes. Then made 2% agarose gel and put 1x TAE buffer with double wells (8 wells, two for 16 wells in total). Then loaded 1kb ladder (very left) the wells on the top represent arthropod amplified samples and the wells on the bottom represent Wolbachia amplified wells. The order goes (for both top and bottom): Ladder, Bug Sample, Positive Arthropod Control, Negative Arthropod Control, Positive DNA control, Negative DNA control.<\/p>\n",Yes,TCATCTCGTGTNGAGAGATTGTGTGTAANGTCGCGTAACGATNTATACTCATNTTTANAGTTGCTATCAGGTCATGGGGG ANACTCCAGAGTNTCNNCNGAGNNANNNTNNTNNANNGTGGATGANGATGNCACATCAANNGGGCTTTATGGAGTGNACT ACNCNNTGCTACANTGGNNTNNACGGGGNGCTNCGTGGNGNGNATGCCTAAACTAATCCAAAANNNTNNCTCTCTTCNNA TTGNACTCTGCTCCACTAGTACATGAAGTTGGAATCNCTAGGTNGNTGNGNNNNATGNCTGGNNAATACGTTTTCNNNTC TTGTACAGACTGATC,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/MD12_AL_A1-Premixed.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/IMG_0215-scaled.jpg,",40.6212742400,-74.5714058899,05/30/2025,"
The bug is terrestrial and was found on the ground in a wooded area. It is tiny, around 3-5mm, and appears to be the larval stage of Drymus crassus.\u00a0<\/em>The upper half of the specimen is black, with the lower half of the body being red with small black spots running down the spine.<\/p>\n",Drymus crassus,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,1X TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/gel.png,","
Extract the DNA using the Qiagen DNeasy extraction kit, and then amplify both\u00a0Wolbachia<\/em> and arthropod COI genes using PCR. After completing PCR, the 2% gel was run using SYBR Safe using a 100bp ladder. In lane 8 of the gel, the sample had a strong band that lined up with the positive Wolbachia<\/em> control in lane 9, which strongly suggests Wolbachia<\/em> presence. The PCR sample was then purified using the QIAquick PCR Purification Kit and sent<\/span>\u00a0out for sequencing.<\/p>\n",Yes,TCATTAAGATGAATTATCCGAATTGAATTAGGACAACCTGGACCATTCATTGAAGACGATCAAATTTATAATACCATTGT AACAGCACACGCATTTATTATAATTTTCTTTATAGTTATACCAATTATAATTGGAGGATTTGGTAACTGATTAGTACCAT TAATAATTGGGGCCCCAGATATAGCATTCCCACGAATAAATAATATAAGATTCTGACTACTCCCACCATCAATCACACTA TTAATCATAAGAAGAATAATCGAAATAGGAGTAGGAACCGGATGAACAGTATATCCTCCTCTATCAAATAACATATTTCA TAGAGGAGCAGCAGTAGATATAGCAATCTTTTCACTACATTTAGCAGGAATATCATCAATTATAGGAGCCATTAATTTCA TCTCAACTATCATTAACATACGACCTACAGGAATAGTACCAGAACAAATTCCTCTATTCGTATGATCAGTAGGAATCACA GCAGTCCTATTATTATTATCATTACCAGTATTGGCCGGAGCAATCACTATACTATTAACAGACCGAAACCTAAATACATC CTTCTTTGACCCTACAGGAGGAGGAGACCCAATTTTATACCAACACTTATTTTGATTTTTTG,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/MD03_ML_A-Premixed.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4883-scaled.jpg,",40.7933949000,-77.8600012000,03/31/2025,"
I found the bug near a bush on a dirty brick pathway, climbing around on the ground, it was sunny, and the bug was by itself.<\/p>\n",Prenolepis imparis,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4950-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4946-scaled.jpg,","
When doing the Wolbachia Gel there was a hole poked into the gel lane 3.<\/p>\n
My Arthropod was in lane 3 of both gels.<\/p>\n",No,CTTCAATAAGAATACTTATCCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTAATTCTATA GTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCC TTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATCAGTT TACTTTTATTAAGAAATTTCATTAATGACGGAGTGGGGACAGGATGAACTGTTTACCCTCCATTAGCTTCTAATATTTTT CATAATGGTCCTTCAGTTGACCTAACAATTTTTTCTCTGCATATTGCAGGGATATCCTCTATTCTAGGAGCTATCAATTT TATTTCAACAATTTTAAATATACGACATAAAAATTTTTCTTCTGATAAAATCCCTTTACTTGTTTGATCTATTTTAATTA CAGCAATTTTACTTCTTCTTTCTTTACCAGTTCTAGCTGGAGCTATTACTATACTTTTAACTGACCGAAACCTTAATACT TCCTTTTTTGATCCCTCAGGAGGGGG,https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/GE2-1-1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/WolbachiaS4-scaled.jpg,",43.49269601617138,-111.98440994555294,04/01/2025,"
Deceased in house, body seems to be older and starting to decompose.<\/p>\n",Lucilia sericata,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Gel2.jpg,","
Lanes 2 and 6 are the Wolbachia and CO1 tests respectively.<\/p>\n",No,CTTGATCCGGATAATTGGAACTTCTTTAAGAATTCTAATTCGAGCTGAATTAGGACATCCTGGAGCTTTAATTGGAGATGATCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTAATGCCAATTATAATTGGAGGATTTGGAAATTGATTAGTTCCATTAATACTAGGAGCTCCAGATATAGCATTCCCTCGAATAAATAATATAAGTTTTTGACTTTTACCTCCTGCATTAACTTTATTATTAGTTAGTAGTATAGTAGAAAACGGAGCTGGAACAGGATGAACAGTTTACCCTCCTCTATCTTCTAATATTGCTCATGGAGGAGCTTCTGTTGATTTAGCTATTTTCTCTCTTCATTTAGCAGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACTACAGTTATTAATATACGATCAACAGGAATTACTTTTGATCGAATACCTTTATTTGTTTGATCAGTAGTAATTACAGCTTTATTACTTTTATTATCATTACCAGTATTAGCAGGAGCTATTACAATACTTTTAACAGACCGAAATCTTAATACATCATTCTTTGACCCTGCAGGAGGAGGTGATCCAATTTTATACCAACATTTATTTTGATTTTTTGGTCACC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/WolbachiaS2-scaled.jpg,",43.45336775689436,-111.95779603617345,04/01/2025,"
Dead in house, unknown for how long but body seemed to be in decent condition.<\/p>\n",Tetramorium caespitum,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Gel1.jpg,","
Lanes 4 and 8 are the Wolbachia and CO1 tests respectively.<\/p>\n",No,TNANTNGCTATTTGAGCAGGAATAATCGGATCTTCAATAAGTATAATTATCCGACTTGAATTAAGTTCCTNTNNTTCATTAATTAATGACGACCAAGTATATAATTCCTTAGTAACAAGACATGCCCTTGTTATAATTTTCTTTATAGTAATGCCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCCATTAATACTAGGAACTCCCGATATAGCCTACCCCCGTATAAATAACATAAGATTTTGACTTCTACCACCCTCTATTAGATTATTATTGCTTAGAAGATTTATTAATCCGGGGGTAGGAACAGGATGAACTATCTATCCCCCATTAGCATCTAATATCTTTCATAGAGGCCCATCAATTGATCTATCAATTTTTTCTCTTCATATTGCAGGAATATCTTCTATTATAGGTGCAATTAATTTTATCGCTACTATTATAAACATACATCATAAAAAATTAACCTTAGATAAAATCCCCCTGTTAGTTTGATCTATTTTAATTACAGCAATTCTTCTCCTCCTATCTCTTCCCGTATTAGCAGGAGCTATTACTATACTCTTAACAGATCGTAATTTAAATACCTCATTTTTTGATCCATCAGGTGGGGGGGACCCAATTCTATATCAACATTTATTCTGATTTTTTGGTCAC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/WolbachiaS1.jpg,",43.492695025962526,-111.98444397908935,04/01/2025,"
Found within house very recently deceased.<\/p>\n",Orthocladius,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/06/Gel1-1.jpg,","
Lanes 3 and 7 are the Wolbachia and CO1 tests respectively.<\/p>\n",No,TTTTGGAGCTTGATCAGGTATAGTAGGAACTTCTCTAAGAATTTTAATTCGAGCTGAATTAGGACATGCCGGTTCATTAATTGGAGATGATCAAATTTATAATGTAATTGTAACTGCTCATGCTTTTGTTATAATTTTTTTTATAGTAATACCAATTTTAATTGGAGGATTCGGAAATTGATTAGTGCCTTTAATATTAGGTGCCCCTGATATAGCATTCCCTCGAATAAATAATATAAGTTTTTGATTATTACCCCCGTCATTGACTTTATTATTATCTAGTTCTATTGTAGAAAATGGAGCTGGGACAGGATGAACGGTTTACCCTCCTCTATCTTCTGGAATTGCTCATGCAGGCGCTTCAGTAGATTTAGCCATTTTTTCTTTACATTTAGCAGGAATTTCATCTATTTTAGGAGCTGTAAATTTTATTACAACAGTTATTAATATGCGGTCTAATGGTATTACTTTAGATCGAATACCTTTATTTGTTTGATCAGTAATTATTACTGCTATTTTATTGCTGCTATCTTTACCAGTATTAGCTGGAGCTATCACAATATTATTAACAGATCGAAATTTAAATACATCATTTTTTGACCCTGCTGGTGGAGGGGATCCTATTCTTTATCAACATTTATTCTGATTTTTTG,,, "",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/image_2025-05-28_114224442.png,",33.8872370000,-118.3525744000,05/13/2025,"
This arthropod was collected from a pot in a garden. The arthropod was then stored in a Petri dish until performing the following procedures.<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-28-110624.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_8568.jpeg,","
Lab2_DNAExtraction_Mar2022<\/a> $\"\"$ $\"\"$ $\"\"$ $\"\"$ <\/p>\n
All lanes not labeled do not contain anything in them.
\nLane 1 Contains the PCR Molecular Weight Ruler.
\nLane 2 Contains the Armadillidium vulgare (H1 Garden Sample)
\nLane 3 Contains the Armadillidium vulgare (H2 Garden Sample)
\nLane 4 Contains the Armadillidium vulgare (S1 School Campus Sample)
\nLane 5 Contains the Armadillidium vulgare (S2 School Campus Sample)
\nLane 6 Contains the Infected drosophila melanogaster Positive Control
\nLane 7 Contains the Uninfected drosophila melanogaster Negative Control
\nLane 8 Contains the positive DNA Control
\nLane 9 Contains Nuclease Free Water<\/p>\n
This specimen’s bands can be located on the labeled lane 2.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_1755.jpeg,",33.8872370000,-118.3525744000,05/13/2025,"
This arthropod was collected from a pot in a garden. Black colored exoskeleton with yellow designs in the center of its body. Segmented body, about 1.75 cm long.<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-28-110624.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_8568.jpeg,","
Lab2_DNAExtraction_Mar2022<\/a> $\"\"$ $\"\"$ $\"\"$ $\"\"$ <\/p>\n
All lanes not labeled do not contain anything in them.
\nLane 1 Contains the PCR Molecular Weight Ruler.
\nLane 2 Contains the Armadillidium vulgare (H1 Garden Sample)
\nLane 3 Contains the Armadillidium vulgare (H2 Garden Sample)
\nLane 4 Contains the Armadillidium vulgare (S1 School Campus Sample)
\nLane 5 Contains the Armadillidium vulgare (S2 School Campus Sample)
\nLane 6 Contains the Infected drosophila melanogaster Positive Control
\nLane 7 Contains the Uninfected drosophila melanogaster Negative Control
\nLane 8 Contains the positive DNA Control
\nLane 9 Contains Nuclease Free Water<\/p>\n
This specimen’s bands can be located on the labeled lane 3.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_4615.jpeg,",33.8624597709,-118.2545857301,05/13/2025,"
This arthropod was taken from the campus of CAMS Highschool, which was then stored in a Petri dish until performing the following procedures. Black colored exoskeleton. Segmented body, about 1.5 cm long. Found under benches in grass.<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-28-110624.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_8568.jpeg,","
Lab2_DNAExtraction_Mar2022<\/a> $\"\"$ $\"\"$ $\"\"$ $\"\"$ <\/p>\n
All lanes not labeled do not contain anything in them.
\nLane 1 Contains the PCR Molecular Weight Ruler.
\nLane 2 Contains the Armadillidium vulgare (H1 Garden Sample)
\nLane 3 Contains the Armadillidium vulgare (H2 Garden Sample)
\nLane 4 Contains the Armadillidium vulgare (S1 School Campus Sample)
\nLane 5 Contains the Armadillidium vulgare (S2 School Campus Sample)
\nLane 6 Contains the Infected drosophila melanogaster Positive Control
\nLane 7 Contains the Uninfected drosophila melanogaster Negative Control
\nLane 8 Contains the positive DNA Control
\nLane 9 Contains Nuclease Free Water<\/p>\n
This specimen’s bands can be located on the labeled lane 4.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-28-105819.png,",33.8614214000,-118.2544495000,05/13/2025,"
This arthropod was taken from the campus of CAMS Highschool, which was then stored in a Petri dish until performing the following procedures. Black colored exoskeleton. Segmented body, about 1.5 cm long. Found under benches in grass.<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-28-110624.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_8568.jpeg,","
Lab2_DNAExtraction_Mar2022<\/a> $\"\"$ $\"\"$ $\"\"$ $\"\"$ <\/p>\n
All lanes not labeled do not contain anything in them.
\nLane 1 Contains the PCR Molecular Weight Ruler.
\nLane 2 Contains the Armadillidium vulgare (H1 Garden Sample)
\nLane 3 Contains the Armadillidium vulgare (H2 Garden Sample)
\nLane 4 Contains the Armadillidium vulgare (S1 School Campus Sample)
\nLane 5 Contains the Armadillidium vulgare (S2 School Campus Sample)
\nLane 6 Contains the Infected drosophila melanogaster Positive Control
\nLane 7 Contains the Uninfected drosophila melanogaster Negative Control
\nLane 8 Contains the positive DNA Control
\nLane 9 Contains Nuclease Free Water<\/p>\n
This specimen’s bands can be located on the labeled lane 5.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/cricket-pic-4.png,",33.7700504000,-118.1937395000,05/01/2025,"
Found in a backyard located in the grass. The temperature was around 64 degrees Fahrenheit.<\/p>\n",Gryllus,DNeasy (Qiagen),Partial abdomen,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/wolbachia-gel-pic-1.png,","
Lane 1: Long Beach cricket 1, Lane 3: Long beach cricket 2, Lane 5: Hawthorne cricket 1, Lane 7: Hawthorne cricket 2<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/cricket-pic-3.png,",33.7700504000,-118.1937395000,05/01/2025,"
Found in a backyard located in the grass. The temperature was around 64 degrees Fahrenheit.<\/p>\n",Gryllus,DNeasy (Qiagen),Partial abdomen,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/wolbachia-gel-pic-1.png,","
Lane 1: Long Beach cricket 1, Lane 3: Long beach cricket 2, Lane 5: Hawthorne cricket 1, Lane 7: Hawthorne cricket 2<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/cricket-pic-2.png,",33.9127807000,-118.3520389000,05/01/2025,"
Found in a group under a basketball hoop in a backyard at night. It was roughly 65 degrees Fahrenheit.<\/p>\n",Gryllus,DNeasy (Qiagen),Partial abdomen,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/wolbachia-gel-pic-1.png,","
Lane 1: Long Beach cricket 1, Lane 3: Long beach cricket 2, Lane 5: Hawthorne cricket 1, Lane 7: Hawthorne cricket 2<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_3582-scaled.jpeg,",33.8595035314,-118.2569681240,05/13/2025,"
caught deceased<\/p>\n",Coleoptera,DNeasy (Qiagen),Whole arthropod,MiniOne,1X TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_7867-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/PNG-image-1-2.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/PNG-image-1.jpeg,","
followed all labs<\/p>\n
lanes (from left to right):<\/p>\n
\n
beetle<\/li>\n
Roly- Poly<\/li>\n
PCR Standard<\/li>\n
Arthropod Positive Control<\/li>\n
Arthropod Negative Control<\/li>\n
DNA Positive Control<\/li>\n
empty<\/li>\n
empty<\/li>\n<\/ol>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_3575-rotated.jpeg,",33.8595237279,-118.2570827078,05/13/2025,"
caught deceased<\/p>\n",Armadillidae,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/PNG-image-1-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_7867-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/PNG-image.jpeg,","
followed all of the lab instructions<\/p>\n
lanes (from left to right):<\/p>\n
\n
beetle<\/li>\n
Roly- Poly<\/li>\n
PCR Standard<\/li>\n
Arthropod Positive Control<\/li>\n
Arthropod Negative Control<\/li>\n
DNA Positive Control<\/li>\n
empty<\/li>\n
empty<\/li>\n<\/ol>\n
<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Beetle-1.jpg,",33.8614214000,-118.2544495000,04/30/2025,"
When caught, the beetle tried to dig out of its container.<\/p>\n",Anisodactylus binotatus,DNeasy (Qiagen),Abdomen,Standard electrophoresis system Bio-Rad,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/WolbachiaGel.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Ladder-Gel.jpg,","
Gel 1
\nLane
\n1 – PCR MW Ladder (failed)
\n2 – centipede (lamyctes emarginatus)
\n3 – pillbug (armadillidium vulgare)
\n4 – woodlouse spider (dysdera crocata)
\n5 – ground beetle (anisodactylus binotatus)
\n6 – Arthropod Positive Control
\n7 – Arthropod Negative Control
\n8 – Drosophila Positive Control
\n9 – Drosophila Negative Control<\/p>\n
Gel 2
\nLane
\n3 – PCR MW Ruler
\n6 – MiniOne Ladder<\/p>\n
Our first gel’s ladder did not run successfully, so we ran another gel with the same ladder and and another MiniOne ladder and compared the relative mobilities of the two gels to determine the base pairs of the first gel.<\/p>\n",No,NNNTNNNNTNTTTGNAGCATGATCAGGAATAGTAGGGACTTCTTTAAGCATATTAATTCGAGCTGAATTAGGAACTCCTG GTGCATTAATTGGTGACGATCAGATTTATAATGTTATTGTTACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATA CCTATTATAATTGGAGGATTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGCATTTCCTCGAATAAA TAATATAAGTTTTTGATTATTACCTCCATCATTAACCCTTCTTTTAATGAGAAGAATGGTTGAAAGAGGAACTGGGACTG GTTGAACAGTTTACCCTCCTTTATCATCTGGTATTGCCCATGGAGGAGCTTCAGTAGATTTAGCTATTTTTAGATTACAT TTAGCTGGAGTATCTTCAATTTTAGGGGCAGTAAATTTTATTACAACAATTATTAATATACGATCAGTAGGTATAACATT TGATCGTATACCATTATTTGTTTGATCAGTAGGAATTACTGCATTACTTTTATTATTATCTTTACCAGTACTAGCAGGAG CAATTACTATATTATTAACAGATCGAAATTTAAATACTTCTTTTTTTTGATCCTGCTGGAGGAGGAGACCCAATTTTATA CCAACATTTATTTTGATTTTTTGGTCACCNGNNAAGTTNNAA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/23_Beetle-CO1F_F05.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Spider-1-scaled.jpg,",33.8614214000,-118.2544495000,04/30/2025,"
This arthropod had a very large abdomen compared to our other samples.<\/p>\n",Dysdera crocata,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/WolbachiaGel.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Ladder-Gel.jpg,","
Gel 1
\nLane
\n1 – PCR MW Ladder (failed)
\n2 – centipede (lamyctes emarginatus)
\n3 – pillbug (armadillidium vulgare)
\n4 – woodlouse spider (dysdera crocata)
\n5 – ground beetle (anisodactylus binotatus)
\n6 – Arthropod Positive Control
\n7 – Arthropod Negative Control
\n8 – Drosophila Positive Control
\n9 – Drosophila Negative Control<\/p>\n
Gel 2
\nLane
\n3 – PCR MW Ruler
\n6 – MiniOne Ladder<\/p>\n
Our first gel’s ladder did not run successfully, so we ran another gel with the same ladder and and another MiniOne ladder and compared the relative mobilities of the two gels to determine the base pairs of the first gel.<\/p>\n",No,NNNNNNNNNNNNNNNTNGNTNGCTTGGTCTGCTATAGTGGGTACAGCAATAAGAGTCTTAATTCGGGCGGAGTTGGGTCA GGTTGGAAGATTATTGGGTGATGATCATTTGTTTAATGTGGTGGTTACAGCGCATGCTTTAGTTATGATTTTTTTTATAG TAATACCTATTATAATTGGAGGATTTGGTAATTGGTTAGTGCCTTTGATGCTTGGAGCTCCTGATATGGCTTTTCCTCGT ATGAATAATTTAAGTTTTTGGTTGTTGCCTCCTTCTTTAATTTTATTAGTTATTTCTTCGATAGTGGAAATAGGGGTGGG GGCTGGGTGAACGATTTATCCCCCCTTGTCAGGGGCATTAGGGCATGCCGGAGTGTCGGTAGATTTAGCTATTTTTAGCT TGCATTTAGCTGGGGCTTCTTCTATTATGGGGGCAATTAATTTTATTTCTACAATTTTGAATATGCGGTCTGAGGGAATA TCTTTGGATAAGGTACCTTTGTTTGTGTGATCTGTTTTGGTAACGGCTGTTTTGTTATTGTTGTCGTTGCCAGTTTTAGC TGGGGCGATTACTATGCTATTAACGGATCGGAATTTTAATACATCTTTTTTTGACCCAGCGGGAGGAGGGGATCCTATTT TGTTTCAACATTTATTTTGATTTTTTGGTCACCNGNNAAGTTNN,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/22_Spider-CO1F_E05.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Pillbug.png,",33.8614214000,-118.2544495000,04/30/2025,"",Armadillidium vulgare,DNeasy (Qiagen),Abdomen,Standard electrophoresis system Bio-Rad,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/WolbachiaGel.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Ladder-Gel.jpg,","
During the process of DNA extraction, there was some difficulty separating the tissue from the exoskeleton, possibly leading to a lower quality extraction.<\/p>\n
Gel 1
\nLane
\n1 – PCR MW Ladder (failed)
\n2 – centipede (lamyctes emarginatus)
\n3 – pillbug (armadillidium vulgare)
\n4 – woodlouse spider (dysdera crocata)
\n5 – ground beetle (anisodactylus binotatus)
\n6 – Arthropod Positive Control
\n7 – Arthropod Negative Control
\n8 – Drosophila Positive Control
\n9 – Drosophila Negative Control<\/p>\n
Gel 2
\nLane
\n3 – PCR MW Ruler
\n6 – MiniOne Ladder<\/p>\n
Our first gel’s ladder did not run successfully, so we ran another gel with the same ladder and and another MiniOne ladder and compared the relative mobilities of the two gels to determine the base pairs of the first gel.<\/p>\n",Yes,NNNNNNNGNNNTGGGGTTTGAGCAGGGGCTGTTGGAACTGCCCTTAGAATAATTATTCGCACTGAATTAGGACAACCTGG AAGTTTAATTGGAGATGACCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATAC CTATTATAATTGGTGGATTTGGTAATTGGTTAATTCCATTAATACTAGGAGCCCCAGATATGGCTTTTCCACGAATAAAT AATATAAGATTTTGACTTTTACCCCCTTCTTTAACTTTATTGCTTAGAAGTGGGTTGGTTGAAAGAGGAGTAGGAACAGG ATGGACAGTATATCCTCCGCTGGCGTCTAGAATTGCTCATAGAGGAGCATCTGTAGATTTAGGTATTTTTTCTCTCCACC TTGCTGGAGCTTCTTCTATTTTAGGGGCTGTGAATTTTATTACTACTATAATTAATATACGAGCAGCTGGAATTAGAATA GACCGTGTTCCTTTATTTGTTTGATCAGTATTAGTAACGGCTGTACTTTTACTTTTATCCCTACCTGTATTAGCAGGAGC TATTACTATATTATTAACTGATCGAAATTTAAATACCTCTTTTTTTGACCCTAGGGGAGGAGGAGACCCTATCCTTTATC AACACTTATTTTGATTTTTTGGTCACCNNGNAGGTTNA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/41_RolyPoly-CO1F_D07-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_9939-scaled.jpeg,",33.86110663762521,-118.2547445007134,04/28/2025,"
Aphid was collected at the California Academy of Math and Science between 55-65 degrees fahrenheit<\/p>\n",Arthropoda,DNeasy (Qiagen),Abdomen,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_5667.jpg,","\n\n\n\n\n\n\n\n\n\n\n\n\n
<\/td>\n lane 2: mm ruler<\/td>\n<\/tr>\n
<\/td>\n lane 3: uni. ruler<\/td>\n<\/tr>\n
<\/td>\n lane 5: long leg spider<\/td>\n<\/tr>\n
<\/td>\n lane 6: roly poly<\/td>\n<\/tr>\n
<\/td>\n lane 7: dragon fly<\/td>\n<\/tr>\n
<\/td>\n lane 8: black aphids<\/td>\n<\/tr>\n
<\/td>\n lane 9: (+) arthro control<\/td>\n<\/tr>\n
<\/td>\n lane 10: (-) arthro control<\/td>\n<\/tr>\n
<\/td>\n lane 11: (+) DNA control<\/td>\n<\/tr>\n
<\/td>\n lane 12: water<\/td>\n<\/tr>\n
<\/td>\n lane 14: mm ruler<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
lane 15: uni ruler<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_9115-scaled.jpeg,",33.86091074334422,-118.25481123583988,04/28/2025,"",Arthropoda,DNeasy (Qiagen),Abdomen,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_5667.jpg,","
\n\n\n\n\n\n\n\n\n\n\n\n\n\n
<\/td>\n lane 2: mm ruler<\/td>\n<\/tr>\n
<\/td>\n lane 3: uni. ruler<\/td>\n<\/tr>\n
<\/td>\n lane 5: long leg spider<\/td>\n<\/tr>\n
<\/td>\n lane 6: roly poly<\/td>\n<\/tr>\n
<\/td>\n lane 7: dragon fly<\/td>\n<\/tr>\n
<\/td>\n lane 8: black aphids<\/td>\n<\/tr>\n
<\/td>\n lane 9: (+) arthro control<\/td>\n<\/tr>\n
<\/td>\n lane 10: (-) arthro control<\/td>\n<\/tr>\n
<\/td>\n lane 11: (+) DNA control<\/td>\n<\/tr>\n
<\/td>\n lane 12: water<\/td>\n<\/tr>\n
<\/td>\n lane 14: mm ruler<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
lane 15: uni ruler<\/p><\/blockquote>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_6345.jpg,",33.8610309,-118.2547536,04/30/2025,"
dragonfly was collected at California Academy of Mathematics and Science. Weather between 55-65 Fahrenheit.<\/p>\n",Arthropoda,DNeasy (Qiagen),Abdomen,MiniOne,,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_5667.jpg,","
lane 2: mm ruler<\/p>\n
lane 3: uni. ruler<\/p>\n
lane 5: long leg spider<\/p>\n
lane 6: roly poly<\/p>\n
lane 7: dragon fly<\/p>\n
lane 8: black aphids<\/p>\n
lane 9: (+) arthro control<\/p>\n
lane 10: (-) arthro control<\/p>\n
lane 11: (+) DNA control<\/p>\n
lane 12: water<\/p>\n
lane 14: mm ruler<\/p>\n
lane 15: uni. ruler<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/DSC10228-1-scaled.jpg,",43.4871498859,-111.9872730833,04/07/2025,"
Sample code: LW16<\/p>\n",Adalia frigida,Edwards Buffer,Whole arthropod,Standard electrophoresis system,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1st_gel.jpg,","",Yes,TGAGCAGGAATAGTAGGAACTTCTCTAAGAATTATTATTCGATTAGAATTAGGTACAACTAACAGACTAATTGGAAATGACCAAATTTATAATGTTATTGTAACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCCATTATAATTGGAGGTTTTGGAAACTGACTAGTACCTTTAATAATTGGAGCGCCTGATATGGCATTCCCACGTCTTAATAATATAAGATTTTGATTATTACCTCCAGCTTTAACCCTTTTAATTTCTAGAAGAGTAATTGAAATAGGAGCAGGTACAGGATGAACAGTATATCCACCTCTTTCTTCAAATATAGCACATAATGGACCTTCTGTAGATTTAGTAATTTTTAGATTACACTTAGCTGGAATTTCATCAATTTTAGGAGCTGTAAATTTTATTTCTACTATTATAAATATACGACCTAATGGGATAAATCTAGATAAAACACCTTTATTTGTTTGATCAGTTTTAATTACAGCTATTCTACTACTTTTATCATTACCAGTTCTTGCAGGAGCAATTACTATATTATTAACAGATCGTAATATTAATACATCATTTTTTGACCCTACTGGAGGAGGGGATCCAGTTTTATACCAACACTTATTTTGATTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/DSC10201-scaled.jpg,",43.5541523,-111.8804601,01/24/2025,"
caught deceased<\/p>\n
sample code: LW5<\/p>\n",,Edwards Buffer,Whole arthropod,Standard electrophoresis system,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1_29_25_Gel_Image_Cropped.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/LW2_2-1-scaled.jpg,",43.48715667629995,-111.98726192224464,01/20/2025,"
caught deceased<\/p>\n
Sample code: LW2<\/p>\n",Pollenia pediculata,Edwards Buffer,Whole arthropod,Standard electrophoresis system,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1_22_25_Gel_Image_Cropped-1.jpg,","",Yes,TTTCGGAGCNTGATCAGGAATAGTAGGTACCTCTTTAAGAATATTAGTTCGAGCAGAATTAGGACATCCTGGAGCATTAATCGGTGATGATCAAATTTATAATGTAATTGTAACAGCCCATGCCTTTGTTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGACTTGTTCCTTTAATATTAGGAGCACCTGATATAGCATTCCCTCGAATGAATAATATAAGTTTTTGACTCTTACCTCCTGCACTTACATTATTATTGGTGAGCAGTATAGTGGAAAACGGAGCTGGGACAGGATGAACTGTTTACCCACCTCTATCTTCTAATATTGCTCATGGAGGGGCTTCTGTTGATTTAGCTATTTTTTCTCTTCATTTAGCAGGAATTTCTTCAATTTTAGGAGCAGTTAATTTTATTACAACTGTAATTAATATACGATCTACAGGTATTACATTTGACCGAATACCTTTATTTGTTTGATCTGTAGTAATTACAGCATTATTACTTCTTTTATCCTTACCTGTATTAGCAGGAGCTATCACTATATTATTAACAGATCGAAATTTAAATACTTCCTTTTTTGATCCTGCAGGAGGAGGAGACCCAATTTTATATCAACATTTATTTTGATTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/LW1_2-1-scaled.jpg,",43.4871685845,-111.9872938453,,"
caught deceased.<\/p>\n",Armadillidium vulgare,Edwards Buffer,Whole arthropod,Standard electrophoresis system,1X TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1_22_25_Gel_Image_Cropped.jpg,","",Yes,TTGGGGTTTGAGCAGGGGCTGTTGGGACTGCCCTTAGAATAATTATTCGCACTGAATTAGGACAGCCTGGAAGTTTAATTGGAGATGATCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATACCTATTATAATTGGTGGATTCGGTAACTGGTTAATTCCATTAATACTAGGAGCCCCAGATATAGCTTTTCCACGGATAAATAATATAAGATTTTGACTTTTACCCCCTTCTTTAACTTTATTACTTAGAAGGGGATTAGTTGAAAGAGGAGTAGGAACAGGGTGGACAGTATATCCTCCGCTGGCGTCGAGAATTGCTCACAGAGGTGCATCTGTAGATTTAGGTATTTTTTCTCTCCACCTTGCTGGAGCTTCTTCTATTTTAGGGGCTGTAAATTTTATTACTACTATAATTAATATACGAGCAGCTGGAATCAGAATAGACCGTGTTCCTTTATTTGTTTGATCAGTAATAGTAACGGCTGTGCTTTTGCTTTTATCATTACCTGTACTAGCAGGAGCTATTACTATATTATTAACTGATCGAAATTTAAATACCTCTTTTTTTGACCCTAGCGGAGGTGGGGATCCTATCCTTTATCAACATTTATTCTGATTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/8B746587-4CB3-4B71-BBE6-736D5CF38CB3.jpg,",33.8611082147,-118.2549120610,05/09/2025,"
Bug was found on the leaves of a bush.<\/p>\n",Murgantia histrionica,DNeasy (Qiagen),Body without legs,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-28-9.21.14-AM.png,","
Gel electrophoresis was run successfully and bands of harlequin bug DNA were present.<\/p>\n",No,NNNNNNNNNNNNNNTGNNNNNNNANANNNGGTATAGTTGGTACTTCTTTAAGAATCCTAATTCGCCTAGAACTAGGAACA ACTAATAGATTAATTGGAAACGACCAAATTTATAATGTAATTGTTACTGCTCATGCATTTATTATAATTTTTTTCATAGT AATACCTATTATAATTGGAGGATTTGGAAATTGACTAGTACCATTAATAATTGGCGCTCCAGATATAGCTTTCCCACGTT TAAATAATATAAGATTTTGACTTTTGCCTCCCGCATTAACATTATTAATAATAAGAATAATTGTAGAAATAGGGGCAGGC ACAGGTTGAACAGTCTACCCTCCACTATCTTCAAATTTGGCACATAATGGACCCTCTGTTGATTTAGTAATTTTTAGTTT ACATTTAGCTGGAATTTCGTCTATTTTAGGGGCTGTTAATTTTATTTCAACTATTATAAATATACGCCCTTCGGGAATAA GATTAGATAAAACTCCCTTATTTGTTTGATCAGTTATGATCACAGCTATTTTATTACTTTTATCTTTACCTGTATTAGCA GGAGCCATCACTATACTTTTAACAGATCGAAATATCAATACTTCATTTTTTGACCCTACGGGAGGGGGAGACCCTATTCT TTACCAACATTTATTCTGATTTTTTGGTCACCCTTGAAANTNNN,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/schoolbug.png,",33.9547370000,-118.2120161000,05/01/2025,"
At home, found in the backyard under mulch.<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen) blood and tissue kit.,Abdomen,MiniPCR,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/COlGel.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/wolbachia.png,","",No,NNNNNNNNNTTTGGGGTTTGAGCAGGGGCTGTTGGAACTGCCCTTAGAATAATTATTCGCACTGAATTAGGACNACCTGG AAGTTTAAATGGAGATGACCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATAC CTATTATAATTGGTGGATTTGGTAATTGGTTAATTCCATTAATACTAGGAGCCCCAGATATGGCTTTTCCACGAATAAAT AATATAAGATTTTGACTTTTACCCCCTTCTTTAACTTTATTGCTTAGAAGTGGGTTGGTTGAAAGAGGAGTAGGAACAGG ATGGACAGTATATCCTCCGCTGGCGTCTAGAATTGCTCATAGAGGAGCATCTGTAGATTTAGGTATTTTTTCTCTCCACC TTGCTGGAGCTTCTTCTATTTTAGGGGCTGTGAATTTTATTACTACTATAATTAATATACGAGCAGCTGGAATTAGAATA GACCGTGTTCCTTTATTTGTTTGATCAGTATTAGTAACGGCTGTACTTTTACTTTTATCCCTACCTGTATTAGCAGGAGC TATTACTATATTATTAACTGATCGAAATTTAAATACCTCTTTTTTTGACCCTAGGGGAGGAGGAGACCCTATCCTTTATC AACACTTATTTTGATTTTTTGGTCACCCTGAAGTTNAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/35_Pillbug-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/D138CD77-4743-4E0F-81B4-556E6F42D48A.jpg,",33.8611823162,-118.2547760815,05/09/2025,"
Insect was found under a discarded piece of wood.<\/p>\n",Neuroptera,DNeasy (Qiagen),Larva,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-28-9.21.14-AM-1.png,","
While a gel was run with Plodia Interpunctella samples\/DNA gel electrophoresis was not run properly\/ samples were not prepared correctly so no bands appeared on the gel.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/2E6305BC-43F2-40E0-88E8-A02D6D15728E.jpg,",33.8611925135,-118.2547222531,05/09/2025,"
Insect was found in leaf litter that came from a nearby tree.<\/p>\n",Forficula auricularia,DNeasy (Qiagen),Rear half,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-28-9.21.14-AM-1.png,","
While a gel was run with Forficula auricularia samples\/DNA gel electrophoresis was not run properly\/ samples were not prepared correctly so no bands appeared on the gel.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/D-nemesis-hen.png,",33.8612330463,-118.2547387536,05/09/2025,"
Insect was found crawling in grass when collected.<\/p>\n",Harmonia axyridis,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-28-9.21.14-AM.png,","
While a gel was run with Harmonia Axyridis samples\/DNA gel electrophoresis was not run properly\/ samples were not prepared correctly so no bands appeared on the gel.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_3006-2.jpeg,",118.2551400000,33.8608000000,05/12/2025,"
\n
The arthropod was caught in a garden.<\/li>\n
It was green and had long legs.<\/li>\n<\/ul>\n",Condylostylus,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_0664-scaled.jpeg,","
The arthropods were caught and then ground to proceed to the DNA extraction and purification process. Once our experimental and control samples were prepped, we moved on to the PCR protocol. Samples and our PCR cocktail were added to each of our PCR tubes, which were then put into our MiniOne PCR machine. Finally, loading dye was added to the separated portions of our PCR samples and was loaded into our gel. Our final gel was stained with 100X Fast Blast and put onto a light box for imaging.<\/span><\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screen-Shot-2025-05-19-at-9.42.26-AM.png,",33.8607775366,-118.2551858141,05/12/2025,"
The pill bug was found in the soil of our school’s garden. It was also attempting to dig into the soil. It had a hard exoskeleton, was about the size of a grain of rice, and was dark gray.<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screen-Shot-2025-05-19-at-9.38.42-AM.png,","
The arthropods were caught and then ground to proceed to the DNA extraction and purification process. We did come across an issue with our second arthropod sample containing the pill bug, as the exoskeleton was not removed to lessen the risk of being unable to extract sufficient DNA. This led to our spin column being clogged for a significant amount of time, which we fixed by moving the sample around with a pipette tip. However, this might have decreased the efficiency of our DNA sample. Once our experimental and control samples were prepped, we moved on to the PCR protocol. Samples and our PCR cocktail were added to each of our PCR tubes, which were then put into our MiniOne PCR machine. Finally, loading dye was added to the separated portions of our PCR samples and was loaded into our gel. Our final gel was stained with 100X Fast Blast and put onto a light box for imaging.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/spider.png,",33.8612294210,-118.2553503156,04/28/2025,"
We found this grass spider in the grass near bushes. It was collected in the late morning, between 50-60 degrees F.<\/p>\n",Agelenidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/wolbachia-gel-edited.png,","
DNA Extraction:\u00a0<\/strong>The arthropod was crushed thoroughly to ensure proper extraction of DNA.<\/p>\n
Gel Electrophoresis Lanes for Arthropod Gel:<\/strong><\/p>\n
1 – PCR molecular ruler (ladder)<\/p>\n
2 – Black-Bodies Fruit Fly<\/p>\n
3 – Roly Poly<\/p>\n
4 – Grass Spider<\/p>\n
5 – Long-Legged Fly<\/p>\n
6 – (+) Arthropod Control<\/p>\n
7 – (-) Arthropod Control<\/p>\n
8 – (+) DNA Control<\/p>\n
Analysis<\/strong>:<\/strong> Our controls worked confirming our trial worked correctly and we had bands confirming that our insect samples are arthropods.<\/p>\n
Gel Electrophoresis Lanes for Wolbachia Gel:<\/strong><\/p>\n
1 – PCR molecular ruler (ladder)<\/p>\n
2 – Black-Bodies Fruit Fly<\/p>\n
3 – Roly Poly<\/p>\n
4 – Grass Spider<\/p>\n
5 – Long-Legged Fly<\/p>\n
6 – (+) Arthropod Control<\/p>\n
7 – (-) Arthropod Control<\/p>\n
8 – (+) DNA Control<\/p>\n
Analysis<\/strong>:<\/strong> Our did not work meaning our trial was not successful and there were no bands on our gel except for our ladder meaning that our run is inconclusive most likely due to Wolbachia primers not working correctly.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/roly-poly.png,",33.8619529226,-118.2547688797,04/28/2025,"
We found this roly poly under some bark and leaves. It was collected in the late morning, between 50-60 degrees F. Many different insects and more roly polies were present but we only collected one.<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen) blood and tissue kit,Rear half,Standard electrophoresis system Bio-Rad,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/wolbachia-gel-edited.png,","
DNA Extraction:\u00a0<\/strong>The arthropod was crushed thoroughly to ensure proper extraction of DNA.<\/p>\n
Gel Electrophoresis Lanes for Arthropod Gel:<\/strong><\/p>\n
1 – PCR molecular ruler (ladder)<\/p>\n
2 – Black-Bodies Fruit Fly<\/p>\n
3 – Roly Poly<\/p>\n
4 – Grass Spider<\/p>\n
5 – Long-Legged Fly<\/p>\n
6 – (+) Arthropod Control<\/p>\n
7 – (-) Arthropod Control<\/p>\n
8 – (+) DNA Control<\/p>\n
Analysis<\/strong>:<\/strong> Our controls worked confirming our trial worked correctly and we had bands confirming that our insect samples are arthropods.<\/p>\n
Gel Electrophoresis Lanes for Wolbachia Gel:<\/strong><\/p>\n
1 – PCR molecular ruler (ladder)<\/p>\n
2 – Black-Bodies Fruit Fly<\/p>\n
3 – Roly Poly<\/p>\n
4 – Grass Spider<\/p>\n
5 – Long-Legged Fly<\/p>\n
6 – (+) Arthropod Control<\/p>\n
7 – (-) Arthropod Control<\/p>\n
8 – (+) DNA Control<\/p>\n
Analysis<\/strong>:<\/strong> Our did not work meaning our trial was not successful and there were no bands on our gel except for our ladder meaning that our run is inconclusive most likely due to Wolbachia primers not working correctly.<\/p>\n
<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/fly.png,",33.8610928319,-118.2553225552,04/28/2025,"
We found this black-bodied fruit fly by sweeping some bushes with a net. It was collected in the late morning, between 50-60 degrees F.<\/p>\n",Drosophila melanogaster,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/arthropod-gel-edited.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/wolbachia-gel-edited.png,","
DNA Extraction:\u00a0<\/strong>The arthropod was crushed thoroughly to ensure proper extraction of DNA.<\/p>\n
Gel Electrophoresis Lanes for Arthropod Gel:<\/strong><\/p>\n
1 – PCR molecular ruler (ladder)<\/p>\n
2 – Black-Bodies Fruit Fly<\/p>\n
3 – Roly Poly<\/p>\n
4 – Grass Spider<\/p>\n
5 – Long-Legged Fly<\/p>\n
6 – (+) Arthropod Control<\/p>\n
7 – (-) Arthropod Control<\/p>\n
8 – (+) DNA Control<\/p>\n
Analysis<\/strong>:<\/strong> Our controls worked confirming our trial worked correctly and we had bands confirming that our insect samples are arthropods.<\/p>\n
Gel Electrophoresis Lanes for Wolbachia Gel:<\/strong><\/p>\n
1 – PCR molecular ruler (ladder)<\/p>\n
2 – Black-Bodies Fruit Fly<\/p>\n
3 – Roly Poly<\/p>\n
4 – Grass Spider<\/p>\n
5 – Long-Legged Fly<\/p>\n
6 – (+) Arthropod Control<\/p>\n
7 – (-) Arthropod Control<\/p>\n
8 – (+) DNA Control<\/p>\n
Analysis<\/strong>:<\/strong> Our did not work meaning our trial was not successful and there were no bands on our gel except for our ladder meaning that our run is inconclusive most likely due to Wolbachia primers not working correctly.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/long-legged-fly.png,",33.8612311299,-118.2553338442,04/28/2025,"
This long-legged fly was found after sweeping some bushes with a net. It was collected in the late morning in 50-60 degree F weather.<\/p>\n",Condylostylus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system Bio-Rad,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/arthropod-gel-edited.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/wolbachia-gel-edited.png,","
DNA Extraction:\u00a0<\/strong>The arthropod was crushed thoroughly to ensure proper extraction of DNA.<\/p>\n
Gel Electrophoresis Lanes for Arthropod Gel:<\/strong><\/p>\n
1 – PCR molecular ruler (ladder)<\/p>\n
2 – Black-Bodies Fruit Fly<\/p>\n
3 – Roly Poly<\/p>\n
4 – Grass Spider<\/p>\n
5 – Long-Legged Fly<\/p>\n
6 – (+) Arthropod Control<\/p>\n
7 – (-) Arthropod Control<\/p>\n
8 – (+) DNA Control<\/p>\n
Analysis<\/strong>:<\/strong> Our controls worked confirming our trial worked correctly and we had bands confirming that our insect samples are arthropods.<\/p>\n
Gel Electrophoresis Lanes for Wolbachia Gel:<\/strong><\/p>\n
1 – PCR molecular ruler (ladder)<\/p>\n
2 – Black-Bodies Fruit Fly<\/p>\n
3 – Roly Poly<\/p>\n
4 – Grass Spider<\/p>\n
5 – Long-Legged Fly<\/p>\n
6 – (+) Arthropod Control<\/p>\n
7 – (-) Arthropod Control<\/p>\n
8 – (+) DNA Control<\/p>\n
Analysis<\/strong>:<\/strong> Our did not work meaning our trial was not successful and there were no bands on our gel except for our ladder meaning that our run is inconclusive most likely due to Wolbachia primers not working correctly.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_5664.jpg,",33.8611020614,-118.2547534232,04/30/2025,"
Spider was collected at California Academy of Mathematics and Science. Weather between 55-65 Fahrenheit.<\/p>\n",Arachnida,DNeasy (Qiagen),Abdomen,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_5667.jpg,","
lane 2: mm ruler<\/p>\n
lane 3: uni. ruler<\/p>\n
lane 5: long leg spider<\/p>\n
lane 6: roly poly<\/p>\n
lane 7: dragon fly<\/p>\n
lane 8: black aphids<\/p>\n
lane 9: (+) arthro control<\/p>\n
lane 10: (-) arthro control<\/p>\n
lane 11: (+) DNA control<\/p>\n
lane 12: water<\/p>\n
lane 14: mm ruler<\/p>\n
lane 15: uni. ruler<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_9378-scaled.jpg,",33.8536990380,-118.3995014165,05/04/2025,"
Sand crab was collected along the Redondo Beach shoreline inside the sand when waves came up (early afternoon). Weather was overcast and around 65 degrees F. No environmental image was taken.<\/p>\n",Decapoda,DNeasy (Qiagen),Abdomen,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_0173-scaled.jpg,","
DNA Ladder in well 1
\nEmerita analoga sample 1 (A) in well 3, sample 2 (B) in well 4, and sample 3 (D) in well 6<\/p>\n
Controls run from wells 10-13 (Arthropod +, Arthropod -, DNA +, and DNA – respectively)<\/p>\n
6X UView stain used for all samples, controls, and ladder. 1X TAE Buffer used in the gel casting and electrophoresis chamber.<\/p>\n",No,NNNNNNNNNNNNNTNNNANNNNNNNCTGGGATAGTGGGCACTTCCTTAAGACTTATTATTCGAGCAGAGTTAGGCCAACC AGGAAGACTAATTGGGGACGACCAGATTTATAATGTCATTGTCACTGCCCACGCATTTGTTATAATTTTCTTTATAGTTA TACCGATCATAATCGGGGGATTTGGTAATTGGCTAGTACCTCTTATATTAGGTGCCCCAGACATAGCTTTTCCGCGTATA AACAATATAAGATTCTGACTTTTGCCCCCTTCCCTAACACTTCTTCTCATGAGTGGAATAGTGGAAAGAGGAGTTGGTAC GGGATGGACAGTTTATCCTCCCCTATCAGCAGCAATCGCCCACGCGGGGGCTTCAGTAGACCTTGGTATTTTCAGACTTC ACCTGGCCGGGGTCTCATCCATTCTAGGAGCAGTGAATTTTATAACTACAGTTATTAATATACGACCCAATGGGATAACA ATGGACCGTATACCTCTATTTGTGTGATCAGTGTTCATTACAGCAATTTTACTTTTACTATCGCTACCAGTTTTAGCGGG AGCTATTACCATGCTTTTGACCGACCGTAANTTAAACACCTCTTTTTTTTGATCCGGCCGGAGGGGGAGACCCAGTTCTA TATCAACACCNGTTCNGATTTTTTGGTCACCCNTGNAAGTNTNNANNGTCNNN,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/31_PacificSandCrab-CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_9379-scaled.jpg,",33.8549196854,-118.3998232816,05/04/2025,"
Sand crab was collected along the Redondo Beach shoreline inside the sand when waves came up (early afternoon). Weather was overcast and around 65 degrees F. No environmental image was taken.<\/p>\n",Decapoda,DNeasy (Qiagen),Abdomen,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_0173-scaled.jpg,","
DNA Ladder in well 1
\nEmerita analoga sample 1 (A) in well 3, sample 2 (B) in well 4, and sample 3 (D) in well 6<\/p>\n
Controls run from wells 10-13 (Arthropod +, Arthropod -, DNA +, and DNA – respectively)<\/p>\n
6X UView stain used for all samples, controls, and ladder. 1X TAE Buffer used in the gel casting and electrophoresis chamber.<\/p>\n",No,NNNNNNNNNNNNNNNCNNAGNNNNNNCTGGGATAGTGGGCACTTCCTTAAGACTTATTATTCGAGCAGAGTTAGGCAAAC CAGGAAGACTAATTGGGGACGACAAGATTTATAATGTCATTGTCACTGCCCACGCGTTTGTTATAATTTTCTTTATAGTT ATACCGATCATAATCGGGGGATTTGGTAATTGGCTAGTACATCTTATATTAGGTGCCCCAGACATAGCTTTTCCACGTAT AAACAATATAAGATTCTGACTTTTGCCCCCTTCCCTAACACTTCTTCTCATGAGTGGAATANTGGAAAGAGGAGTTGGTA CGGGATGGACAGTTTATCCTCCCANATCATCANCTCTCGCCTATTGGANGGCTTGCCNAGCCCTTGGTATTTATTGATTT CNNTCCCTAANNNNAGCATCATTATNGGANNNGGGANTTTTATTTNACTNACATTTTTATTTATTANATGTATTNCC,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/32_PacificSandCrab-CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_9380-scaled.jpg,",33.8537435875,-118.3997374509,05/04/2025,"
Sand crab was collected along the Redondo Beach shoreline inside the sand when waves came up (early afternoon). Weather was overcast and around 65 degrees F. No environmental image was taken.<\/p>\n",Decapoda,DNeasy (Qiagen),Abdomen,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_0173-scaled.jpg,","
DNA Ladder in well 1
\nEmerita analoga sample 1 (A) in well 3, sample 2 (B) in well 4, and sample 3 (D) in well 6<\/p>\n
Controls run from wells 10-13 (Arthropod +, Arthropod -, DNA +, and DNA – respectively)<\/p>\n
6X UView stain used for all samples, controls, and ladder. 1X TAE Buffer used in the gel casting and electrophoresis chamber.<\/p>\n",No,NNNNNNNNNCNCNNNNNNNNNNNNCTGGGATAGTGGGCACTTCNNNNAGACTTATTATTCGAGCAGAGTTAGGCCAACCA GGAAGACTAATTGGGGACGACCAGATTTATAATGTCATTGTCACTGCCCACGCATTTGTTATAATTTTCTTTATAGTTAT ACCGATCATAATCGGGGGATTTGGTAATTGGCTAGTACCTCTTATATTAGGTGCCCCAGACATAGCTTTTCCACGTATAA ACAATATAAGATTCTGACTTTTGCCCCCTTCCCTAACACTTCTTCTCATGAGTGGAATAGTGGAAAGAGGAGTTGGTACG GGATGGACAGTTTATCCTCCCCTATCAGCAGCAATCGCCCACGCGGGGGCTTCAGTAGACCTTGGTATTTTCAGACTTCA CNNGGCCGGGGTCTCATCCATTCTAGGAGCAGTGAATTTTATAACTACAGTTATTAATATACGACCCAATGGGATAACAA TGGACCGTATACCTCTATTTGTGTGATCAGTGTTTCATTACAGCAATTTTACTTTTACTATCGCTACCANTTTTAGCGGG AGCTATTACCATGCNTTTNGACCGATCGTAATTTAAACACCTCTTTTTTTTGATCCGGCCNGGAGGGGGGAGACCCCAGT TCNANANCAACACCTGTTTCTGANTTTTTTGGGTCACCNNGAANNTTAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/33_PacificSandCrab-CO1F.fasta,, "",39.8827229986,-75.7195956164,05/09/2025,"",Attulus fasciger,tweezers,Whole arthropod,MiniOne,TBE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-05-at-12.31.41 PM-3.png,","",Yes,,,, "",39.8467767000,-75.7116032000,05/09/2025,"",Coccinellidae,tweezers,Abdomen,MiniOne,TBE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-05-at-12.31.41 PM-2.png,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/homebug.jpg,",33.8316745000,-118.2816930000,05/01/2025,"
On the surface of school garden, found underneath leaves.<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen) blood and tissue kit.,Abdomen,MiniPCR,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/COlGel.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/wolbachia.png,","
N\/A<\/p>\n",No,NNNNNNNNGNNTNNGNGGTTTGAGCAGGGGCTGTTGGAACTGCCCTTAGAATAATTATTCGCACTGAATTAGGACAACCT GGAAGTTTAATTGGAGATGACCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTAAT ACCTATTATAATTGGTGGATTTGGTAATTGGTTAATTCCATTAATACTAGGAGCCCCAGATATGGCTTTTCCACGAATAA ATAATATAAGATTTTGACTTTTACCCCCTTCTTTAACTTTATTGCTTAGAAGTGGGTTGGTTGAAAGAGGAGTAGGAACA GGATGGACAGTATATCCTCCGCTGGCGTCTAGAATTGCTCATAGAGGAGCATCTGTAGATTTAGGTATTTTTTCTCTCCA CCTTGCTGGAGCTTCTTCTATTTTAGGGGCTGTGAATTTTATTACTACTATAATTAATATACGAGCAGCTGGAATTAGAA TAGACCGTGTTCCTTTATTTGTTTGATCAGTATTAGTAACGGCTGTACTTTTACTTTTATCCCTACCTGTATTAGCAGGA GCTATTACTATATTATTAACTGATCGAAATTTAAATACCTCTTTTTTTGACCCTAGGGGAGGAGGAGACCCTATCCTTTA TCAACACTTATTTTGATTTTTTGGTCACCNNNNNNNTTNA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/34_Pillbug-CO1F.fa,, "",39.8467767,-75.7116032,,"",Drosophila melanogaster,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniOne,TBE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-21-2.21.30-PM.png,","
Our controls and DNA ladder passed through the gel correctly and had the expected results.\u00a0 We have also spent many months perfecting our technique and perfecting and mistakes.\u00a0 This fruit fly was a cultivated control that was infected with Wolbachia, and tested positively for Wolbachia.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Armadillidium-vulgare-1.png,",33.8607762370,-118.2552319000,05/02/2025,"",Armadillidium vulgare,DNeasy (Qiagen),Whole arthropod,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-19-175851.png,","
5. PCR molecular weight ruler (1000, 700, 500, 200, 100 bp)<\/p>\n
6. Water<\/p>\n
7. <\/em>Positive DNA Control<\/p>\n
8. DNA sample of Armadillidium vulgare<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/fruitfly-1.jpg,",39.8467767,-75.7116032,05/01/2025,"",Drosophila melanogaster,tweezers,Whole arthropod,MiniOne,TBE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-05-at-12.31.41 PM-1.png,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_6780-scaled.jpeg,",33.8609101000,-118.2553106000,05/05/2025,"
Black, has many legs, looks like pillbug<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_1245-scaled.jpg,","
Armadillidium vulgare of interest is in the fourth lane from the left. PCR with Wolbachia 16S primers was performed, so the band indicates that the organism was positive for Wolbachia.<\/span><\/p>\n",Yes,NNNNNNNNNNNNNNNNNNNNNNNGCAGGGGCTGTTGGAACTGCCCTTAGA ATAATTATTCGCACTGAATTAGGACAACCTGGAAGTTTAATTGGAGATGA CCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTT TTATAGTAATACCTATTATAATTGGTGGATTTGGTAATTGGTTAATTCCA TTAATACTAGGAGCCCCAGATATGGCTTTTCCACGAATAAATAATATAAG ATTTTGACTTTTACCCCCTTCTTTAACTTTATTGCTTAGAAGTGGGTTGG TTGAAAGAGGAGTAGGAACAGGATGGACAGTATATCCTCCGCTGGCGTCT AGAATTGCTCATAGAGGAGCATCTGTAGATTTAGGTATTTTTTCTCTCCA CCTTGCTGGAGCTTCTTCTATTTTAGGGGCTGTGAATTTTATTACTACTA TAATTAATATACGAGCAGCTGGAATTAGAATAGACCGTGTTCCTTTATTT GTTTGATCAGTATTAGTAACGGCTGTACTTTTACTTTTATCCCTACCTGT ATTAGCAGGAGCTATTACTATATTATTAACTGATCGAAATTTAAATACCT CTTTTTTTGACCCTAGGGGAGGAGGAGACCCTATCCTTTATCAACACTTA TTTTGATTTTTTGGTCNNNTNGGAAGTTNNA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Armadillidium-vulgare-1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_1185-scaled.jpeg,",33.860908047834975,-118.25527120617723,05/05/2025,"
Appears to be a pillbug<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_1245-scaled.jpg,","
Armadillidium vulgare of interest is in the fifth lane from the left. PCR with Wolbachia 16S primers was performed, so the band indicates that the organism was positive for Wolbachia.<\/p>\n",Yes,NNNTNNNNNNNNNNNNNNNNNNNANAGCAGGGGCTGTTGGAACTGCCCTT AGAATAATTATTCGCACTGAATTATGGACAACCTGGAAGTTTAATTGGAG ATGACCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATT TTTTTTATAGTAATACCTATTATAATTGGTGGATTTGGTAATTGGTTAAT TCCATTAATACTAGGAGCCCCAGATATGGCTTTTCCACGAATAAATAATA TAAGATTTTGACTTTTACCCCCTTCTTTAACTTTATTGCTTAGAAGTGGG TTGGTTGAAAGAGGAGTAGGAACAGGATGGACAGTATATCCTCCGCTGGC GTCTAGAATTGCTCATAGAGGAGCATCTGTAGATTTAGGTATTTTTTCTC TCCACCTTGCTGGAGCTTCTTCTATTTTAGGGGCTGTGAATTTTATTACT ACTATAATTAATATACGAGCAGCTGGAATTAGAATAGACCGTGTTCCTTT ATTTGTTTGATCAGTATTAGTAACGGCTGTACTTTTACTTTTATCCCTAC CTGTATTAGCAGGAGCTATTACTATATTATTAACTGATCGAAATTTAAAT ACCTCTTTTTTTGACCCTAGGGGAGGAGGAGACCCTATCCTTTATCAACA CTTATTTTGATTTTTTGGTCACCCNTGAAGTTNA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Armadillidium-vulgare-2.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Armadillidium-vulgare-2.png,",33.8607762370,-118.2552319000,05/02/2025,"",Armadillidium vulgare,DNeasy (Qiagen),Whole arthropod,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-19-175943-1.png,","
1. PCR molecular weight ruler (1000, 700, 500, 200, 100 bp)<\/p>\n
2. DNA sample of Pholcus phalangioides<\/p>\n
3. DNA sample of Pholcus phalangioides<\/em><\/p>\n
4. DNA sample of Armadillidium vulgare<\/p>\n
5. Duplex positive control<\/p>\n
6. Duplex negative control<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_2277-scaled.jpeg,",33.8614214000,-118.2544495000,04/30/2025,"",Isopoda,DNeasy (Qiagen),Abdomen,Standard electrophoresis system Bio-Rad,1X TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screen-Shot-2025-05-19-at-1.17.56-PM.png,","",No,NNNNNNNNNNNNNNNNGGTANGANNNNNNGTNNNTGAGGTGCTTGCNCANT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/42_SpotlessLadybugPillBug-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-14-at-11.38.31 AM.png,",33.8610309,-118.2547536,04/30/2025,"
Location: CAMS bushes<\/p>\n
Season: Spring<\/p>\n
Temperature: 62\u00b0F<\/p>\n
Habitat: Terrestrial<\/p>\n
\n
pointy tail<\/li>\n
attatched on leaf<\/li>\n
very still<\/li>\n<\/ul>\n",Coleoptera,tweezers,Abdomen,Standard electrophoresis system Bio-Rad,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-19-at-1.05.03 PM.png,","",No,NNNNNNNNNNGNNTTTGGGGTTTGAGCAGGGGCTGTTGGAACTGCCCTTAGAATAATTATTCGCACTGATTTTTGGGTCN CCCTGAATTTNANTGGAGATGACCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTA ATACCTATTATAATTGGTGGATTTGGTAATTGGTTAATTCCATTAATACTAGGAGCCCCAGATATGGCTTTTCCACGAAT AAATAATATAAGATTTTGACTTTTACCCCCTTCTTTAACTTTATTGCTTAGAAGTGGGTTGGTTGAAAGAGGAGTAGGAA CAGGATGGACAGTATATCCTCCGCTGGCGTCTAGAATTGCTCATAGAGGAGCATCTGTAGATTTAGGTATTTTTTCTCTC CACCTTGCTGGAGCTTCTTCTATTTTAGGGGCTGTGAATTTTATTACTACTATAATTAATATACGAGCAGCTGGAATTAG AATAGACCGTGTTCCTTTATTTGTTTGATCAGTATTAGTAACGGCTGTACTTTNACTTTTATCCCTACCTGTATTANCAG GAGCTATTACTATATTATTAACTGATCGAAATTTAAATACCTCTTTTTTTGACCCTAGGGGAGGAGGAGACCCTATCCTT TATCAACACTTATTTTGATTTTTTGGTCACCCTTGANNTTTAAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-21-at-10.58.01 AM.png,",33.8614214,-118.2544495,04/30/2025,"
\n
found on bush<\/li>\n
located in leaf at the top of the bush<\/li>\n<\/ul>\n",Diptera,DNeasy (Qiagen),Abdomen,Standard electrophoresis system Bio-Rad,1X TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screen-Shot-2025-05-19-at-1.17.56-PM.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_1184-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_1183-scaled.jpeg,",33.8609007857,-118.2552553841,05/05/2025,"
Striped abdomen, red eyes, fly<\/p>\n",Minettia flaveola,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_1245-scaled.jpg,","
Minettia flaveola of interest is in the third lane from the left. PCR with Wolbachia 16S primers was performed, so the lack of band indicates that the organism was negative for Wolbachia.<\/p>\n",No,NNNNNNNNNNNNNNNNGNNNNNNNNNNCCGGATAGTAGGAACATCTTTAA GAATTTTAATTCGTGCTGAATTAGGACACCCAGGTGCCTTAATTGGAGAC GACCAAATTTATAATGTTATTGTTACAGCCCATGCATTTGTTATAATTTT TTTTATAGTTATACCAATTATAATTGGAGGATTTGGAAATTGACTAGTCC CTTTAATATTAGGAGCTCCTGATATGGCATTTCCACGAATAAATAATATA AGATTTTGATTATTACCTCCTTCCCTTACATTACTTTTAGTAAGAAGTAT AGTTGAAAACGGAGCTGGAACAGGATGAACTGTATACCCTCCGTTATCAT CAGGAATTGCTCACGGAGGAGCATCTGTTGATTTAGCTATTTTTAGTCTT CATTTAGCTGGGGCTTCATCAATTATAGGAGCAGTAAATTTTATTACTAC AGTCATTAACATACGATCAACTGGAATCACTTTTGATCGAATACCTTTAT TTGTATGATCTGTTGTAATTACTGCTATTCTTTTACTTCTTTCTCTACCT GTTTTAGCAGGAGCAATCACTATGTTATTAACAGATCGAAATTTAAATAC TTCATTTTTTGACCCAGCGGGTGGAGGAGATCCAATTTTATACCAACATT TATTTTGATTTTTTGGTCACCNNNGNANTTNNN,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Minettia-flaveola.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_6783-scaled.jpeg,",33.860910387859064,-118.25530367879078,05/05/2025,"
shiny green fly<\/p>\n",Condylostylus,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_1245-scaled.jpg,","
Condylostylus of interest is in the second lane from the left. PCR with Wolbachia 16S primers was performed, so the band indicates that the organism was positive for Wolbachia.<\/span><\/p>\n",Yes,NNNNNNNNNNNNCNNNNNNNNNNNNNNNNGGATAATTGGTACATCCCTTA GAATTATTGTACGAGCTGAATTAGGACATCCAGGAGCTCTAATTGGAGAT GACCAAATTTATAATGTTATTGTAACAGCTCACGCATTTATCATAATTTT CTTTATAGTAATACCAATCATAATTGGAGGGTTTGGAAATTGATTAGTTC CCCTTATATTAGGAGCTCCTGATATAGCATTCCCACGAATAAATAATATA AGATTCTGAATACTCCCCCCATCATTAACCCTACTACTAGCTAGTAGTAT AGTAGAAAATGGAGCAGGAACAGGATGAACAGTATATCCCCCTTTATCAG CAGGAATTGCTCACGGAGGTGCATCTGTTGACTTAGCAATTTTTTCTCTT CATTTAGCTGGTATTTCATCTATTTTAGGAGCAGTAAATTTTATTACAAC AGTAATTAATATACGATCAACAGGAATCACTCTAGATCGAATACCATTAT TTGTATGATCAGTAGTAATTACAGCTGTTCTTCTTCTCCTTTCATTACCA GTTCTAGCCGGAGCTATTACCATATTACTAACTGACCGAAATTTAAATAC TTCATTTTTTGACCCAGCAGGAGGAGGTGACCCAATTCTATATCAACATT TATTTTGATTTTTTGGTCACNCNNNANNNTNNN,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Condylostylus.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Pholcus-phalangioides-2.png,",33.8607762370,-118.2552319000,05/02/2025,"
1. PCR molecular weight ruler (1000, 700, 500, 200, 100 bp)<\/p>\n
2. DNA sample of pholcus phalangioides<\/p>\n
3. DNA sample of pholcus phalangioides<\/em><\/p>\n
4. DNA sample of armadillidium vulgare<\/p>\n
5. Duplex positive control<\/p>\n
6. Duplex negative control<\/p>\n",Pholcus phalangioides,DNeasy (Qiagen),Body without legs,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-19-175943.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/cricket-pic.png,",33.9127807000,-118.3520389000,05/01/2025,"
Found in a group under a basketball hoop in a backyard at night. It was roughly 65 degrees Fahrenheit.<\/p>\n",Gryllus,DNeasy (Qiagen),Partial abdomen,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/wolbachia-gel-pic-1.png,","
Lane 1: Long Beach cricket 1, Lane 3: Long beach cricket 2, Lane 5: Hawthorne cricket 1, Lane 7: Hawthorne cricket 2<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_0438.jpeg,",33.8863314000,-118.2935891000,05/14/2025,"
I found this cellar spider below a mirror on the wall in a household bathroom. I collected it at about 8 am.<\/p>\n
$\"\"$ <\/p>\n
<\/p>\n",Pholcus phalangioides,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-20-9.34.48-AM-1.png,","
Gel electrophoresis lanes<\/strong>
\n1. PCR molecular weight ruler (1000, 700, 500, 200, 100 bp)
\n2. DNA sample of pholcus phalangioides
\n3. DNA sample of sphaerophoria scripta<\/em>
\n4. Wolbachia-positive (16S) arthropod control
\n5. Wolbachia-negative (16S) arthropod control
\n7. Arthropod-positive (CO1) DNA control
\n8. Arthropod-negative (CO1) DNA control<\/p>\n
Analysis<\/strong><\/p>\n
The pholcus phalangioides sample had a band matching the Wolbachia-positive control’s band (16S), but not a band matching the arthropod-positive control’s upper band (CO1; like how the arthropod-negative control had no bands), so pholcus phalangioides seems to be a non-arthropod and Wolbachia-positive.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_0440.jpeg,",33.8863314000,-118.2935891000,05/14/2025,"
I found this long hoverfly in an open compost bag with fruit and vegetable scraps buried in soil. I collected it at about 5 pm when it was about 68 degrees Fahrenheit.<\/p>\n",Syrphidae,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,100X Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-20-9.34.48-AM-1.png,","
Gel electrophoresis lanes<\/strong>
\n1. PCR molecular weight ruler (1000, 700, 500, 200, 100 bp)
\n2. DNA sample of pholcus phalangioides
\n3. DNA sample of sphaerophoria scripta<\/em>
\n4. Wolbachia-positive (16S) arthropod control
\n5. Wolbachia-negative (16S) arthropod control
\n7. Arthropod-positive (CO1) DNA control
\n8. Arthropod-negative (CO1) DNA control<\/p>\n
Analysis<\/strong><\/p>\n
The sphaerophoria scripta sample had a band matching the arthropod-positive control’s upper band (CO1), but not a band matching the Wolbachia-positive control’s band (16S; like how the Wolbachia-negative control had no bands), so sphaerophoria scripta seems to be an arthropod and Wolbachia-negative.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/download.jpg,",39.8467767000,-75.7116032000,,"",Chironomus tentans,tweezers,Whole arthropod,MiniOne,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-05-at-12.31.28 PM.png,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_3707.png,",33.8612302770,-118.2553553772,04/30/2025,"",Coleoptera,DNeasy (Qiagen),Abdomen,MiniOne,1X TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screen-Shot-2025-05-19-at-1.17.56-PM.png,","",Yes,NNNNNNNNNNNNNNNNNNNNTNNNNNNNNNGNNTGNCGNNTCNCNNNGAANNNGNNNNNNNNNGGNNANNNNGNNNCCNN NNNNNNTTNNNNNCATCCTNNTCTTACCNAATTGCTCACGGNGGNAGAATGNCCCCGTCACTGCCCCTGTAAGAGTGTNG GNGGNNGGNGNTCAAATATNATCGGGNGTTGGGGGTGGGTGATNGACNNCATNCATTNGAGGCTATNANATANTCTTTTN NTTNACTAACTAAAGTAANAANNTATCTTANTACCCCNNCTTTATTTTTTATTGNTTACNANNAANTTGGTGTGAANNNN ATTAATANCNAGANGNNTNNTTNTTTTTTTTGCTGGTNTTTTNTTCNTTTTTTTTTTTTTTANCNNNNNNCNNNNGNNNN NNNNNTNNNNNTTANNNTANNNATNNANNNTTTNNTNNNN,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/28_RolyPolySpotlessLadybug-CO1F-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Pholcus-phalangioides-1-1.png,",33.8607762375,-118.2552319790,05/02/2025,"",Pholcus phalangioides,DNeasy (Qiagen),Body without legs,MiniOne,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/59C5A188-23D1-4AAF-A63E-A88CB7E79450.jpg,","
1. PCR molecular weight ruler (1000, 700, 500, 200, 100 bp)<\/p>\n
2. DNA sample of Pholcus phalangioides<\/p>\n
3. DNA sample of Pholcus phalangioides<\/em><\/p>\n
4. DNA sample of Armadillidium vulgare<\/p>\n
5. Duplex positive control<\/p>\n
6. Duplex negative control<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/B8_tanytarsus_1-scaled.jpg,",46.5481898880,9.9003661667,10/02/2024,"",Tanytarsus pallidicornis,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Gel_B8_tanytarsus_bearbeitet-scaled.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/B8_Co1-F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/B4_camponotus.jpg,",46.5487359087,9.9006665742,10/02/2024,"",Camponotus herculeanus,Instagene Matrix,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Gel_B4_camponotus_bearbeitet-scaled.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/B4_Co1-F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/centi2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/centi.jpg,",33.8614214000,-118.2544495000,04/30/2025,"
Found under shrubbery, in a damp and dark environment.<\/p>\n",Lamyctes emarginatus,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system Bio-Rad,1X TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/WolbachiaGel.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Ladder-Gel.jpg,","
Gel 1
\nLane
\n1 – PCR MW Ladder (failed)
\n2 – centipede (lamyctes emarginatus)
\n3 – pillbug (armadillidium vulgare)
\n4 – woodlouse spider (dysdera crocata)
\n5 – ground beetle (anisodactylus binotatus)
\n6 – Arthropod Positive Control
\n7 – Arthropod Negative Control
\n8 – Drosophila Positive Control
\n9 – Drosophila Negative Control<\/p>\n
Gel 2
\nLane
\n3 – PCR MW Ruler
\n6 – MiniOne Ladder<\/p>\n
Our first gel’s ladder did not run successfully, so we ran another gel with the same ladder and and another MiniOne ladder and compared the relative mobilities of the two gels to determine the base pairs of the first gel.<\/p>\n",No,NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNTTGNNACNNCNCNNNNNNNNNNTNNNNATCCACCNNCCCCNNNGN NGGNATTCTTNTGGGACACACGTAATTGTCTGGACACTANTCGCCCCCACNCTTTAANTANNATTTTGTNTGGATTACGT CCATCATAAATGGNGTTTGGAAGNNGGCACTTNCNTNTACATCGGTGGNGCNGACGGNNNCCCCNTCNNCCAAATAAAAA ACTAATNTATTAGTANCNCCCCCCCACTCANTN,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/21_Centipede-CO1F_D05.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Bild_2025-04-30_150532360.png,",47.4596592677,9.0194997356,03/19/2025,"
unknown<\/p>\n",Ochlerotatus sticticus,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Image-11.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Bild_2025-04-30_150054179.png,",47.4592977760,9.0228878876,03/19/2025,"
unknown<\/p>\n",Myrmica rubra,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,Cyber Stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Image-10.jpg,","",Yes,AGAATAATTATTCGTKTAGAATTAGGATCTTGYAACCCATTAATTAATAATGATCAAATTTATAATTCTCTTGTAACTAGCCATGCCTTTATTATAATTTTTTTTATAGTTATACCATTTATAATTGGGGGCTTTGGTAATTTTTTAATTCCTTTAATATTAGGATCACCTGATATAGCATACCCACGAATAAATAACATAAGATTTTGATTACTTCCCCCTTCAATTATACTATTAATATTAAGAAATTTTTTAAGGAATGGTGTAGGTACAGGATGAACCATTTACCCCCCTCTAGCTTCTAATATTTTCCATAGAGGACCTTCAATTGATATATCAATCTTTTCTCTTCATATCGCTGGAATATCATCTATTTTAGGTGCTATTAATTTTATCTCAACAATTATTAATATACATCATAAATCAGTATCAATAGACAAAATCCCCTTAATAGTCTGATCAATTTTAATCACAGCTATTCTTCTCTTACTTTCCTTACCAGTCTTAGCAGGAGCAATTACTATATTATTAACAGATCGTAATTTAAATACTTCTTTTTTCGACCCATCTGGGGGGGGAGATCCAATTTTATACCAACATCTATTTTGATTTTTTGGTCACCTGAAAAGTTWAA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/DS1_CO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Bild-20250319-150222-1e237a68.jpeg,",47.4597798902,9.0191428750,03/19/2025,"
I found the Lasius niger nearby woodstacks in the forest directly by a path. The weather was sunny and it was around 15 degrees outside. The place was very dry and full of wood chips. It was in the afternoon, about 3pm. There were a lot of leaves around the place, where I found the Lasius niger. And it was autumn.<\/p>\n",Lasius niger,Instagene Matrix,Body without legs,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Biologie_Wolbachiapraktikum_Elektrophorese.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/NB2_CO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Bild-20250319-145528-5c952cc7.jpeg,",47.4597841871,9.0195362068,03/19/2025,"
I found this the Pterostichus niger under a trunk in the forest near a path. I collected it in the afternoon, about 3pm. It was a sunny day and it was about 15 degrees outside. The area around the trunk was very dirty and wet. The Pterostichus niger was hiding in the dirt 5cm underneath the ground.<\/p>\n
<\/p>\n",Pterostichus niger,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Biologie_Wolbachiapraktikum_Elektrophorese.jpg,","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/NB1_CO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Chelidurella-acanthopygia-FS-1-1.jpg,",47.4602413801,9.0190972767,03/19/2025,"
Forest ground<\/p>\n",Chelidurella acanthopygia,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Gel-Image-FS-LR-1.jpg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Lasius-Niger-Internet.jpg,",47.4603800401,-350.9809385283,03/19/2025,"
Forest ground<\/p>\n",Lasius niger,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Gel-Image-FS-LR-1.jpg,","",No,ACTAGAATTAAGTTCTTCTAATTCATTAATTAATAATGATCAAATTTATAATTCTATAGTTACAAGACATGCATTCGTTATAATTTTCTTTATAGTTATGCCTTTCATAATTGGAGGATTTGGAAATTTTCTCGTACCTTTAATATTAGGATCACCTGATATAGCATACCCCCGTATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATTTCTCTCCTTCTTTTAAGAAATTTTATTAATGACGGAGAGGAACAGGATGAACTGTTTATCCTCCATTAGCTTCTAATATTTTTCATAATGGCCCTCAGTTGATTTAACTATTTTTTCTCTTCATATTGCTGGA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/FS2_CO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BildFM2.1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BildFM2.2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BildFM2.3.jpg,",47.4592753789,9.0234843045,03/19/2025,"
unknown<\/p>\n",Anoecia fulviabdominalis,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/ElektroFM.jpeg,","
Band is not interpretable<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BildFM1.1-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BildFM1.2-2.jpg,",47.4593078345,9.0229746466,03/19/2025,"
unknown<\/p>\n",Ochlerotatus sticticus,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/ElektroFM.jpeg,","
<\/p>\n
Band is not interpretable<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/AMs2025d-scaled.jpg,",41.7756400000,-88.0970000000,09/03/2024,"
collected by Fall 2024 students<\/p>\n",Bombus impatiens,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-07-at-11.00.39 PM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/crayfish.jpg,",43.466503654165706,-112.07092282778625,04/01/2025,"
Subject was caught alive in a freshwater aquatic (pond) environment.<\/p>\n",Pollenia pediculata,Edwards Buffer,Partial abdomen,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/PCR2.jpeg,","
The sample gel electrophoresis tests are located in the bottom gel, rightmost column, fourth and eighth rows (WspecF and CO1, respectively) (see gel EP image). The shakiness of the bars in the Gel EP suggests imperfections in the gel structure, most likely during formation.<\/p>\n
The DNA extraction was performed on the reproductive organs of the sample (female).<\/p>\n",No,CCCTGGAGCATTAATCGGTGATGATCAAATTTATAATGTAATTGTAACAGCCCATGCCTTTGTTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGACTTGGAAATTGACTTGTTCCTTTAATATTAGGAGCACCTGATATAGCATTCCCTCAAATGAATAATATAATTTTTTGACTCTTACCTCCTGCNCTNACTTTATTATTGGTGAGCAGTATAGTGGAAAACGGAGCTGGGACAGGATGAACTGTTTACCCACCTCTATCTTCTAATATTGCTCATGGAGGGGCTTCTGTTGATTTAGCTATTTTTTCTCTTCATTTAGCAAGAATNTCTTCTCTTTTAGGAGCAGTTAATTTTATTACNACTGTATTTAATATACNATCTACAGGTATTACATTTGACCGCNTACCTTTATTTGTTTTATCTGTANTAATTACNGCCTTATTAATTTTT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/ZL6_CO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/spider.jpg,",43.4233213000,-112.0143728000,04/01/2025,"
Caught alive in a suburban environment, isolated from its web.<\/p>\n",Tegenaria,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/PCR2.jpeg,","
The\u00a0Tegenaria<\/em> tests are located in the bottom gel, rightmost column, third and seventh rows (WspecF and CO1, respectively). The shakiness of the bars in the Gel EP suggests imperfections in the gel structure, most likely during formation.<\/p>\n",No,AAAGTGATGATTCTAATTGAATTGGGTCAGTTGGGGAGATTTATTGGAGATGATCATTTATATAATGTAATTGTTACGGCTCACGCTTTTGTTATGATTTTTTTTATAGTGATACCTATTATAATTGGTGGGTTTGGAAATTGATTGGTTCCTTTAATATTAAGGGCTCCGGATATGGCTTTTCCTCGAATGAATAATTTGAGATTTTGATTGTTGCCTCCTTCGTTATTTATGCTTTTTATTTCTTCTATGGTGGATATAGGGGTTGGTGCTGGTTGAACAATTTATCCTCCTTTGGCTTCTTCTATGGGACATATAGGGAGAGCTATAGATTTTGCTATTTTTTCTTTGCATTTAGCAGGGGCGTCTTCTATTATGGGAGCGGTGAATTTTATTACTACGATTTTAAATATACGTTCTGTAGGGATAAGAATAGATAGGGTTTCTTTGTTTGTTTGGTCGGTATTGGTTACCGCTATTTTATTGTTATTGTCTTTGCCTGTATTAGCGGGTGCTATTACGATATTGTTGACGGATCGAAATTTTAATACATCATTTTTTGATCCTGCAGGTGGAGGGGATCCGATTTTATTTCAACACTTGTTTTGATTTTTTGGTCACCCTGAA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/ZL5_CO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/whitefly_egg.jpg,",43.4233213000,-112.0143728000,04/01/2025,"
Was found inhabiting a lemon tree in a residential suburban environment. Found and captured in egg stage.<\/p>\n",Trialeurodes vaporariorum,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/PCR2.jpeg,","
The\u00a0Trialeurodes vaporariorum<\/em> tests are located in the bottom gel, rightmost column, second and sixth rows (WspecF and CO1, respectively). The shakiness of the bars in the Gel EP suggests imperfections in the gel structure, most likely during formation.<\/p>\n",No,ATGTTCTTGTAACTTCCCATGCATTTATTATAATTTTTTTTATGACTATGCCTCTTGTTATTGGTGGGTTCGGGAACTGGCTGGTTCCTCTTATGGTTGGGGCTCCTGATATGGCGTTTCCTCCAATAAATAATCTTAGATTTTGGCTGTTGNTTCCTTCTTTGTTTTTTATGCTTGTTAGACTTTTGATTGATAGGGGAAGCGGCACGGGTTGAACTGTTTATCCCCCCCTATCTATAAGGCTGTCACATAGGGGGAATTCTGTCGATTTTTCTATTATNTCTTTGCACGTTGCTGGAATTTCTTCTATTTTGGGATCACTTAATTTTATCCTGACTATTGTAAACATGCAGGCGTTGGGCATAAAAATGGAGTTTTAATCTTTGTTTGTCTGATCTGTGTTTATTACTGTTTTCTTGCTTTTAATTTCTCTTCCTGTGTGGGCAGGGGCAATTACTATACTTTTACTGGATNGTAATTTTAA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/ZL4_CO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Ameise-1-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Ameise-2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Ameise-3.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Ameise-4.jpg,",474620.3200000000,90132.8100000000,03/05/2025,"
I found it in a little forest, about 3 pm, in a field path with gravel, at a sunny spot. It was about 10-15 degrees. I didn’t find alot of them, just one. The one I collected<\/p>\n",Lasius niger,Instagene Matrix,Body without legs,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Gel-1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Assel-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Assel-2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Assel-3.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Assel-4.jpg,",474620.3200000000,90132.8100000000,03/05/2025,"
I found it at a little forest. It was about 3 pm, with 10-15 degrees. Under a dry conifer in the shadow. There were alot of them there.<\/p>\n",Porcellio scaber,Instagene Matrix,Rear half,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Gel-2.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Image-13.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Image-14.jpg,",474620.3200000000,90132.8100000000,03/05/2025,"
I found it in a little forest. It was at about 3pm. It was under a conifer bark. It was 10 to 15 degrees. There were 4 of this bugs but I took one.<\/p>\n",Rhagium inquisitor,Instagene Matrix,Rear half,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Gel-bearbeitet.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1000005745-scaled.jpg,",43.4807784,-112.0117657,,"",Isopoda,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/isopod2-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/isopod.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1000005741-scaled.jpg,",43.4233213000,-112.0143728000,,"",Chironomus riparius,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/harlequin.jpg,","",No,TGGGAACTTCATTAAGAATGCTTATTCGAGCAGAATTAGGACGACCCGGAACTTTCATTGGAGATGACCAAATTTATAATGTTGTAGTTACTGCACATGCTTTTATTATAATTTTTTTCATAGTTATACCAATTTTAATTGGAGGATTCGGAAACTGACTTGTCCCCCTAATACTTGGAGCACCTGACATAGCTTTTCCTCGAATAAATAATATAAGTTTCTGACTTTTACCCCCCTCTCTTACTCTTCTTCTTTCTAGTTCTTTCGTAGAAAATGGAGCAGGAACTGGGTGAACTGTTTATCCACCTCTATCATCAGCAATTGCTCATAGAGGAGCTTCTGTTGACTTAGCTATTTTTTCTTTACATTTAGCTGGAATTTCATCAATTCTTGGATCAGTAAATTTTATTACTACAGTTATTAATATGCGAGCTAATGGTATTACACTTGATCGAATACCTTTATTTGTCTGATCAGTAGTAATTACAACTGTTCTTTTACTTCTTTCTCTTCCAGTCTTAGCTGGAGCCATTACTATACTATTAACAGACCGAAATTTAAATACTTCATTTTTTGATCCTGCTGGTGGGGGTGATCCTATTTTATACCAACACTTATTTTGATT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1000005743-scaled.jpg,",43.4233213,-112.0143728,,"",Pterostichus,Edwards Buffer,Abdomen,Standard electrophoresis system,TAE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/beetle.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1000005742-scaled.jpg,",43.4233213000,-112.0143728000,04/13/2025,"",Forficula auricularia,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Earwig.jpg,","",No,GGCTTGATCAGGGATAGTAGGGACTTCATTGAGTTTGTTGATTCGAGCAGAGTTGGGACAACCTGGAGCTTTAATTGGAGACGATCAAATTTATAATGTAATTGTTACGGCCCACGCTTTCGTAATGATTTTTTTTATGGTAATGCCTATTATGATTGGGGGTTTTGGGAATTGATTGGTACCCCTGATGCTGAGAGCCCCTGACATGGCCTTTCCTCGAATAAATAATATAAGTTTTTGGTTACTACCTCCTTCGCTTATACTTTTGCTCTCTGGGAGAATGGTAGACAGAGGGGCAGGAACGGGTTGGACGGTCTACCCTCCCCTGTCGGGGGCTATCGCTCACGCAGGGGCTTCGGTGGATTTGAGAATTTTTTCATTGCACTTGGCGGGGGTTTCATCCATTTTAGGGGCAATTAATTTTATTACAACGGTGATTAACATGCGCCCCTCAGGTCTTAAATTGGAACGAATACCATTATTTGTGTGATCTGTAGCCATTACTGCTCTTTTATTGTTACTTTCTTTACCAGTGTTGGCAGGGGCTATCACTATGCTTTTGACAGACCGGAATTTAAATACATCTTTTTTTGACCCTGCGGGAGGGGGGGACCCTATTCTCTATCAACATTTATTTTGATTTTTTG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1000005661-scaled.jpg,",43.4233213000,-112.0143728000,,"",Chironomus riparius,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/chiron.jpg,","",No,GNATAGTGGGAACTTCATTAAGAATGCTTATTCGAGCAGAATTAGGACGACCCGGAACTTTCATTGGAGATGACCAAATTTATAATGTTGTAGTTACTGCACATGCTTTTATTATAATTTTTTTCATAGTTATACCAATTTTAATTGGAGGATTCGGAAACTGACTTGTCCCCCTAATACTTGGAGCACCTGACATAGCTTTTCCTCGAATAAATAATATAAGTTTCTGACTTTTACCCCCCTCTCTTACTCTTCTTCTTTCTAGTTCTTTCGTAGAAAATGGAGCAGGAACTGGGTGAACTGTTTATCCACCTCTATCATCAGCAATTGCTCATAGAGGAGCTTCTGTTGACTTAGCTATTTTTTCTTTACATTTAGCTGGAATTTCATCAATTCTTGGATCAGTAAATTTTATTACTACAGTTATTAATATGCGAGCTAATGGTATTACACTTGATCGAATACCTTTATTTGTCTGATCAGTAGTAATTACAACTGTTCTTTTACTTCTTTCTCTTCCAGTCTTAGCTGGAGCCATTACTATACTATTAACAGACCGAAATTTAAATACTTCATTTTTTGATCCTGCTGGTGGGGGTGATCCTATTTTATACCAACACTTATTTTGA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/1000005662-scaled.jpg,",43.4807879,-112.0117695,04/01/2025,"",Steatoda triangulosa,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/steatoda-1.jpg,","",No,ATAATTGGAACAGGTATGAGTGTTTTAATTCGGGTAGAATTAGGACAATCTGGAAGATTGTTAGGTGATGATCAATTATATAATGTAATTGTTACTGGACACGCTTTTGTGATGATTTTTTTTATAGTAATACCAGTATTAATTGGTGGATTTGGAAATTGGTTGGTTCCATTAATATTAGGGGCTCCTGATATAGCTTTTCCTCGAATAAATAATTTAAGATTTTGATTATTACCTCCTTCTTTATTATTATTATTAATTTCTTCTATAGAAGAAATAGGTGTTGGAGCTGGATGAACTATTTACCCCCCTTTATCTTCTCTGGAAGGTCATAGAGGAAGATCTGTAGATTTTGCTATTTTTTCTTTACATTTGGCTGGTGCTTCTTCCATTATAGGTGCTATTAATTTTATTTCTACTATTTTAAATATACGTTTATTTGGGATGAGAATAGAAAAAGTTAGACTTTTTGTTTGGTCAGTATTAATCACTGCAGTATTATTATTATTATCTTTACCTGTATTGGCAGGTGCCATTACTATATTATTAACAGATCGAAATTTTAATACTTCTTTTTTTGACCCGGCTGGGGGAGGTGATCCAATTTTATTTCAACATTTGTTTTGATTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Isopod-Elisabet-Cabrera-Caballero-27-Feburary-2-scaled.jpg,",38.8196500000,-77.2619567000,03/09/2025,"
Brown pillbug with pale yellow-beige accents caught in an urban forest during spring.<\/p>\n",Trachelipus rathkii,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Latin-III.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Screenshot-2025-05-06-at-8.48.41 PM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/DSC10233-scaled.jpg,",43.4233213000,-112.0143728000,04/01/2025,"
Arthropod was collected alive in an interior suburban environment.<\/p>\n",Chrysoperla,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/RPCR1-1.jpg,","",Yes,ATTTGATCTGGATTAGTAGGTACAAGATTAAGTTTATTGATTCGAGCTGAATTAGGTCAACCAGGTTCTTTAATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTAATACCTATTGTAATTGGAGGTTTTGGTAATTGATTAGTTCCTTTAATACTAGCTGCTCCTGATATAGCTTTTCCACGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTTTATTACTTGCTTCTTCTATAGTAGAAAGAGGAGCTGGGACTGGTTGAACAGTTTACCCTCCTTTATCATCAAGAATTGCACATGCTGGAGCTTCTGTGGATTTAGCTATTTTTAGTTTACACCTTGCCGGAATCTCATCAATTTTAGGTGCAGTAAATTTCATTACAACAGTAATTAATATACGATTAAGTTATATAACATTAGACCGTATACCATTATTTGTATGATCAGTAGTAATTACAGCTTTATTATTATTACTTTCATTACCTGTATTAGCGGGTGCTATTACTATGTTATTAACTGATCGTAATTTAAATACTTCATTTTTTGATCCTGCAGGAGGAGGAGATCCAATTTTATATCAACATTTATTTTGATTTTTTGGTCACCC,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/ZL2_CO1-1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/DSC10232-scaled.jpg,",43.4233213000,-112.0143728000,04/01/2025,"
Found inside of my house, recently deceased, surrounded by several others.<\/p>\n",Harmonia axyridis,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/RPCR1.jpg,","",No,TTGGATATGGGCAGGAATAGTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAGAGGAAGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTTTGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTTTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGATAAAACACCTTTATTTGTATGATCTGTTCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCATTTACTATACTATTAACTGAC,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/ZL1_CO1.fasta,, "",38.8176997269,-77.1685620830,,"",,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Mahnoor-K_IMG_0001-scaled.jpg,",41.7756400000,-88.0970000000,09/03/2024,"
Collected by Fall 2024 students.<\/p>\n",Formicidae,DNeasy (Qiagen) blood and tissue kit.,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Team-4C.jpg,","",Yes,TATCGTTGGTTGATTCAGAATCGGGTCTCCCCTCCTGAAGGGATCAAAAAATGAAGTATTTAAGTTTCGATCAGTTAATAATATTCGTAATAGCTCCAGCTAAAACGGGAAGGGATAATAAAAGTAAAATTCGCAGTGATTAAGATAGATCATACAAGTAGGGGAATTTTATCAATAGAAAAATTTTTATGATGTATATTAAGGATTGTTGAAATAAAATTAATAGCTCCTAAAATAGATGATATACCGGCAATATGAAGAGAAAAAATTGTTAAGTCTACAGAAGGCCCATTATGAAAAATATTTGAAGCTAAAGGAGGGTAGATAGTTCACCCAGTTCCAGTACCATCATTAATAAAGTTTCTTAAAAGTAATAGAGTGATGGAAGGAGGTAAAAGTCAAAATCTTATATTATTTATACGAGGATAAGCTATATCTGGCGATCCTAATATTAAAGGAATTAGAAAATTTCCAAATCCACCAATTATAAATGGTATTACTATAAAAAAGATTATAATAAAGGCATGATTAGTTACTAAAGAATTATAAATTTGATCATTATTAATTAATGAATTTGAAGAACCTAATTCTAATCGAATAATTATACTTATAGAAGATCCAATTATTCCAGCTCAGATAGCAAATAAAAAATAAAGAATTCCAATATCTTTATGTT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Insect_MKAnthropod.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hemiptera-Varsha-Ayyala-12-March-1.jpg,",38.86270297268756,-77.11699497314451,,"
Freshwater stream in a forested area in the backyard of a house and scooped\u00a0up from the stream with a net.<\/span><\/p>\n",Decapoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImageVvAeg6-scaled.jpg,",37.2451640000,-78.4529740000,10/31/2024,"",Chauliognathus pensylvanicus,promega wizard SV,,Standard electrophoresis system,0.25 TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Slide1.jpeg,","
A 1% agarose at 110V for 30 minutes, all well contained<\/span>10 \u03bcL<\/span>.<\/span> 1) ESO arthropod (CO1) PCR reaction, 2)\u00a0 ESO <\/span>Wolbachia<\/span><\/i> (W) PCR reaction, 3) Kendra CO1 PCR reaction, 4) Kendra W PCR reaction, 5) Saad CO1 PCR reaction, 6) Saad W PCR reaction, 7) Kassidy CO1 PCR\u00a0 reaction 8) Kassidy W PCR reaction 9) Positive control from both groups 10) 100bp NEB Ladder.\u00a0<\/span><\/p>\n

<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Pure-green-sweat-bee-scaled.jpg,",37.2516400000,-78.4529740000,10/31/2024,"
It was caught at a wildflower field near a lake in the Fall.<\/p>\n",Augochlora pura,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-28-at-9.20.47 AM.png,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/processed-7DBDD935-BB8E-4300-8152-F986DF1D15C3.jpeg,",37.245164,-78.452974,10/31/2024,"
Wildflowers near Lake Mayes at Hampden-Sydney Campus<\/p>\n
Season: Fall<\/p>\n
Temperature: 78\u00b0F<\/p>\n
Habitat: Terrestrial<\/p>\n",Atalopedes campestris,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screen-Shot-2025-04-14-at-9.32.34-AM.png,","
DNA extraction<\/strong>: The abdominal contents were removed and crushed thoroughly.<\/p>\n
Gel electrophoresis lanes for CO1 arthropod and Wolbachia<\/em> 16S rRNA PCR<\/strong>:<\/p>\n
1: 1000bp ladder<\/p>\n
2-7: other people’s DNA<\/p>\n
8: arthropod primers<\/p>\n
9:\u00a0Wolbachia <\/em>primers<\/p>\n
10: control DNA<\/p>\n
All of the controls worked.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Carpender-Bee-scaled.jpg,",37.2451640000,-78.4529540000,10/31/2024,"
Large black and yellow bee with a large abdomen and thorax. Closely resemble a bumblebee with yellow and black markings and hind legs.<\/p>\n",Hymenoptera,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Wolbachia-Ladder.jpg,","
Wolbachia was not found in the arthropod sample. There was a PCR inhibitor that resulted in failure for DNA to amplify in PCR which is seen through no bands appearing for the arthropod sample.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/A83BE4A8-FFC3-4C77-A9D3-84568B8F8547-scaled.jpeg,",37.245164,-78.452954,10/31/2024,"",Orthoptera,DNeasy (Qiagen),Abdomen,MiniPCR,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-14-at-9.34.14-AM.png,","
DNA extraction: I made sure to crush the arthropod well, I dissected out the abdomen and excluded the exoskeleton.<\/p>\n
Minor issues: During the DNA extraction I had to spin down my sample multiple times due to the consistency of the extracted DNA, the washes were not working and we were eventually able to spin it down and wash it after multiple attempts.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3200-scaled.jpeg,",37.245164,-78.452874,10/31/2024,"
The collection of samples took place in the fall when the weather was cooler. The terrestrial habitat (a lake) allowed for a good specimen collection.\u00a0<\/span><\/p>\n",Apis mellifera,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Slide1.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/ArthropodPhylogeny.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImagehGbw0e-scaled.jpg,",37.24212321878305,-78.45578638001656,10/31/2024,"
Found in the fall in the wildflowers near lake Mayes, a terrestrial habitat at a temperature of 78\u00baF<\/p>\n",Atalopedes campestris,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Slide1-1.jpg,","
DNA extraction: I made sure to crush the arthropod really well dissecting the abdomen and not the exoskeleton.<\/p>\n
Gel Electrophoresis Lanes for CO1 Arthropod:<\/p>\n
1- 1000bp ladder<\/p>\n
2- Arthropod positive PCR Product<\/p>\n
3- Wolbachia negative PCR Product<\/p>\n
10- Control DNA<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0927.jpg,",37.2451640000,-78.4529740000,12/13/2030,"",,DNeasy (Qiagen),Abdomen,MiniPCR,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot_28-4-2025_93059_docs.google.com_.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0322-scaled.jpeg,",37.2451640000,-78.4529740000,10/31/2024,"",Enallagma civile,promega wizard SV,body without wings,Standard electrophoresis system,TAE,Ethidium Bromide,"","
The whole body (minus wings) was used and this caused the filter to get clogged, and did not allow the DNA to amplify.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/huronsachem.jpg,",37.2451640000,-78.4629740000,10/31/2024,"
Fall<\/p>\n
78 Degrees Fahrenheit<\/p>\n
Found in wildflowers near Lake Mayes<\/p>\n",Atalopedes huron,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/wolbachiagel.png,","",No,ATTAGGAACTTCTTTAAGATTATTAATTCGAACAGAACTAGGTAATCCTGGATCTTTAATTGGAGATGATCAAATTTATAATACTATCGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGATTTGGAAATTGATTAGTACCATTAATATTAGGAGCCCCTGATATAGCTTTCCCACGAATAAATAACATAAGATTTTGAATATTACCTCCTTCATTAACATTATTAATTTCAAGAAGAATTGTAGAAAATGGAGCAGGAACAGGATGAACTGTTTATCCTCCTTTATCCTCAAACATTGCTCATCAAGGATCTTCTGTTGATTTAGCTATTTTTTCTCTTCATTTAGCTGGAATTTCATCTATTTTAGGAGCTATTAATTTTATTACAACAATCATTAATATACGAATTAAAAATTTATCATTTGATCAAATACCTTTATTTGTATGATCTGTAGGAATTACAGCATTACTATTATTGTTATCTTTACCTGTATTAGCAGGAGCTATTACTATATTACTTACAGATCGAAATT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_8361-scaled.jpeg,",37.34385169999999,-78.4069732,10/31/2024,"",Palpada vinetorum,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Gel2-Figure.jpg,","",No,NNNNNNGNNTTTGGTACNTGAGCAGGTATAGTAGGAACTTCATTAAGAATTTTAATTCGAGCTGAACTAGGTCATCCAGGTGCATTAATTGGTGATGATCAAATTTATAATGTTATTGTAACAGCTCATGCTTTTGTAATAATTTTCTTTATAGTAATACCTATTATAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCTCCTGATATAGCATTCCCTCGAATAAATAATATAAGTTTTTGATTATTACCTCCTTCTTTAACTTTATTATTAGTAAGAAGTATAGTAGAAAATGGAGCTGGAACAGGATGAACAGTTTATCCTCCTTTATCAAGTAATATTGCTCATGGAGGAGCTTCAGTTGATTTAGCAATTTTCTCACTTCATTTATCTGGTATATCATCAATTTTAGGGGCTGTAAATTTTATTACAACAGTTATTAATATACGATCTACAGGTATTACATATGATCGAATACCTTTATTTGTTTGATCTGTTGCTATTACTGCATTATTATTATTACTTTCATTACCAGTATTAGCTGGAGCTATTACTATATTATTAACAGATCGAAATTTAAATACAACATTTTTTGATCCTGCAGGTGGTGGAGATCCAATTTTATACCAACATTTATTTTGATTTTTTGNNCNNCNNNNAANTTTAAN,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/asian-lady-beetle-pic.png,",37.2426032,-78.4605072,10/31/2024,"",Harmonia axyridis,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Wolbachia-project-gel.png,","
The KOCO1 well is positive for CO1 gene<\/p>\n
The KOW well is negative for Wolbachia<\/p>\n
The control DNA well is the positive control for CO1 gene and 16s rRNA gene<\/p>\n
The last well is the 1000 bp DNA ladder<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6519-scaled.jpeg,",37.2451640000,-78.4529740000,10/31/2025,"
The arthropod was collected on fall day with a temperature of 78\u00ba F. Found near a lake with flowers.<\/p>\n",Ischnura posita,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-14-at-9.39.28 AM.png,","
DNA extraction: I made sure to crush the abdomen well. I dissected the abdomen out and made sure to not include the exoskeleton.<\/p>\n
Gel Electrophoresis lanes for CO1 arthropod PCR.<\/p>\n
1.100bp Ladder<\/p>\n
8. The tenth well was my arthropod sample.<\/p>\n
9. The ninth well was my wolbachia sample.<\/p>\n
10. The tenth well contained the positive and negative PCR controls.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5081-scaled.jpeg,",37.245164,-78.452974,10/31/2024,"
The arthropod was caught near wildflowers Lake Mayes. It has large eyes and abdomen with large wings that extend past the abdomen. It was mostly black with a little yellow on the thorax.<\/p>\n",Xylocopa,DNeasy (Qiagen),Abdomen,,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BIOL-324-Arthropod-Gel.jpg,","
DNA extraction: The arthropod was cut at the abdomen and the contents were grounded with a pestle thoroughly.<\/p>\n
Loading Gel Lanes:<\/p>\n
lane 1- 100bp DNA ladder<\/p>\n
lane 2-7- other group members’ samples<\/p>\n
lane 8-9- my arthropod and Wolbachia<\/em> samples<\/p>\n
lane 10- DNA positive control<\/p>\n
<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/eastern-tailed-blue.jpg,",37.245164,-78.452974,10/31/2024,"
Location: Wildflower field near Lake Mayes<\/p>\n
Season: Fall<\/p>\n
Temperature: 78 degrees F<\/p>\n
Habitat: Terrestrial<\/p>\n",Lycaenidae,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Gel-5-with-labeling.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Short-horned-grasshoppper-image-scaled.jpg,",37.245164,-78.452974,10/31/2024,"",Melanoplus,promega wizard SV,Abdomen,Standard electrophoresis system,0.25 TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-14-at-9.35.15-AM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_1097.jpg,",37.3011371773,-78.3985835871,10/31/2024,"
Fall, 78 deg F<\/p>\n",Scolia dubia,Wizard SV (Promega),Partial abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Slide2.jpg,","
In a 1.5 mL microfuge tube, the specimen and 275 \u00b5L of prepared Digestion Solution Master Mix, containing proteinase K, RNase A Solution, and 0.5 M EDTA. A pestle was used to physically grind the specimen and placed in a 55 \u2103 water bath for 15 minutes for cell lysis. The sample was centrifuged for 60 seconds at 2000 x <\/span>g <\/span><\/i>and transferred to a new 1.5 mL microfuge tube with 250 \u00b5L of Wizard SV Lysis Buffer. The lysate sample was centrifuged for 3 minutes at 13,000 x <\/span>g. <\/span><\/i>The column was washed with 650 \u00b5L of Wizard SV Wash and centrifuged for 60 seconds at 13,000 x <\/span>g. <\/span><\/i>This wash process was repeated four times and 50 \u00b5L of 65 \u2103 nuclease-free water was added. The eluted samples were stored at -20 \u2103.\u00a0<\/span><\/p>\n
Polymerase Chain Reaction<\/b><\/p>\n
The Arthropod PCR Cocktail was prepared in a 1.5 mL microcentrifuge tube with adequate measurements (Table 2), cortexed and centrifuged for proper mixing. Two 0.2 mL PCR tubes were tabled with control and extracted specimen DNA. In each 0.2 mL tube, 23 \u00b5L of the Arthropod PCR Cocktail was added and centrifuged. In the corresponding labeled PCR tubes, 2 \u00b5L of control and extracted specimen DNA was added. The samples were transferred to a BioRad Thermal Cycler and set for 2 minutes at 94<\/span>\u00b0C for initial denaturation, 30 cycles at 30:45:60 second intervals at 94:49:72\u00b0C respectively, final extension was for 10 minutes at 72\u00b0C and held at 4\u00b0C. The 0.2 mL PCR tubes were stored in <\/span>-20 \u2103.\u00a0<\/span><\/p>\n
<\/p>\n\n\n\n\n\n\n\n\n\n\n
Table 2.<\/b>\u00a0<\/span><\/p>\n
Arthropod PCR Cocktail<\/span><\/td>\n
Chemical\u00a0<\/span><\/td>\n Volume<\/span><\/td>\n<\/tr>\n
<\/td>\n Arthropod Primer F\/ R<\/span><\/td>\n 5 \u00b5L<\/span><\/td>\n<\/tr>\n
<\/td>\n Water<\/span><\/td>\n 16.25 \u00b5L<\/span><\/td>\n<\/tr>\n
<\/td>\n Taq Master Mix, 2X<\/span><\/td>\n 31.25 \u00b5L<\/span><\/td>\n<\/tr>\n
Wolbachia PCR\u00a0<\/span><\/td>\n <\/td>\n <\/td>\n<\/tr>\n
<\/td>\n Wolbachia Primer F\/ R<\/span><\/td>\n 5 \u00b5L<\/span><\/td>\n<\/tr>\n
<\/td>\n Water<\/span><\/td>\n 16.25 \u00b5L<\/span><\/td>\n<\/tr>\n
<\/td>\n Taq Master Mix, 2X<\/span><\/td>\n 31.25 \u00b5L<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
<\/p>\n
The 16s rRNA <\/span>Wolbachia <\/span><\/i>primer was prepared in a 1.5 mL PCR tube with the adequate measurements (Table 2). Two 0.2 mL PCR tubes were tabled with control and extracted specimen DNA. In each 0.2 mL tube, 23 \u00b5L of the <\/span>Wolbachia <\/span><\/i>primer mix was added and centrifuged. In the corresponding labeled PCR tubes, 2 \u00b5L of control and extracted specimen DNA was added. The samples were transferred to a BioRad Thermal Cycler and set for 2 minutes at 94<\/span>\u00b0C for initial denaturation, 30 cycles at 30:45:60 second intervals at 94:55:72\u00b0C respectively, final extension was for 10 minutes at 72\u00b0C and held at 4\u00b0C. The 0.2 mL PCR tubes were stored in <\/span>-20 \u2103.\u00a0<\/span><\/p>\n
Gel Electrophoresis<\/b><\/p>\n
A 1% agarose solution was prepared with 50 mL 1x TAE buffer and 0.5 g agarose microwaved. After the solution was clear, 5 \u00b5L of 10mg\/mL ethidium bromide was added and then solidified in 30 minutes. The gel was covered 1 cm by 1x TAE Buffer prior to sample loading. el loading order is shown in Table 3.\u00a0<\/span><\/p>\n
<\/p>\n\n\n\n\n\n\n\n
Table 3. <\/b>Gel Loading Order<\/span><\/td>\n Sample<\/span><\/td>\n Volume added<\/span><\/td>\n<\/tr>\n
Lane 1<\/span><\/td>\n 100 bp NEB ladder<\/span><\/td>\n 10 \u00b5L<\/span><\/td>\n<\/tr>\n
Lane 4<\/span><\/td>\n Arthropod sample<\/span><\/td>\n 10 \u00b5L<\/span><\/td>\n<\/tr>\n
Lane 5<\/span><\/td>\n Wolbachia<\/span><\/i><\/td>\n 10 \u00b5L<\/span><\/td>\n<\/tr>\n
Lane 10<\/span><\/td>\n Positive <\/span>Wolbachia <\/span><\/i>and positive arthropod samples<\/span><\/td>\n 5 \u00b5L each<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
The gel was run at 110 V for 30 minutes. DNA amplicon visualization is conducted in a UV SYNGENE Gel documentation system. System lighting is adjusted to capture the band sizes. The gel was disposed of in a biohazard waste container. PCR samples were sent to Applied Biosystems for Sanger sequencing.\u00a0<\/span><\/p>\n
Bioinformatic Analysis<\/b><\/p>\n
Gel electrophoresis results were submitted to The Wolbachia Project database. Data input guidelines are provided by the database. Arthropod and <\/span>Wolbachia <\/span><\/i>PCR samples underwent Sanger sequencing at Applied Biosystems. Chromatogram data was received back in <\/span>.ab1<\/span><\/i> format and examined in <\/span>Benchling<\/span><\/i> software. Sequence realignment trimmed reads with a QS value under 40 on the 5\u2019 and 3\u2019 end. A FASTA file was created following realignment and was imported to Primer-BLAST (National Library of Medicine 2024). Core_nts database was used in Primer-BLAST. The discrepancy of BLAST genetic identification and <\/span>Seek <\/span><\/i>identification will be discussed later. Phylogenetic analysis was conducted in <\/span>NGPhylogeny.fr<\/span><\/i> (Lemoine et al. 2019) and sample CO1 gene sequences were provided for 11 arthropod species.\u00a0<\/span><\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/image0-77-rotated.jpeg,",37.2451640000,-78.4529740000,10/31/2024,"
Gathered around some wildflowers near a lake when it was 78F.<\/p>\n",Bombus impatiens,Monarch DNA extraction (NEB),Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Wolbachia-gel.png,","",No,,,, "",37.2451640000,-78.4528740000,10/31/2024,"",Hymenoptera,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-26-211816.png,",37.2451640000,-78.4529740000,10/31/2024,"
2 wings, small, 6 legs, black and orange yellow coloring<\/p>\n",Hymenoptera,promega wizard SV,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-14-094005.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/42B5EDFA-E93D-4B61-B35E-D6E7DF4BD4B0-1.png,",41.77564,-88.0970,09/03/2024,"
collected by Fall 2024 students<\/p>\n",Chauliognathus pensylvanicus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/gel--scaled.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/762CB284-FE31-4300-ADE2-7E8D328E019E.jpeg,",36.1444896000,-86.8011462000,09/04/2024,"
Collected by Fall 2024 students<\/p>\n",Chauliognathus pensylvanicus,DNeasy (Qiagen) blood and tissue kit.,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_7650-scaled.jpeg,",41.77564,-88.0970,09/03/2024,"
Collected by Fall 2024 students.<\/p>\n",Melanoplus differentialis,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-17-at-12.59.38 PM.png,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/45-D_AP4_Co1_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5541.jpeg,",41.7784418000,-88.0952683000,09/03/2024,"
Small sized Bald Faced Hornet with yellow spotting across thorax and face with the overall insect a deep black color. Skinny waist with a pair of translucent wings.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-26-at-3.54.59 PM.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3665.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3665-1.jpeg,",41.7756400000,-88.0970000000,09/03/2024,"
collected by Fall 2024 students<\/p>\n",Chauliognathus pensylvanicus,DNeasy (Qiagen) blood and tissue kit.,Rear half,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3668.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/NAs2025d2-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/NAs20251-3-scaled.jpg,",41.77741616290782,-88.09621273750696,11/12/2030,"
collected by Fall 2024 students<\/p>\n",Odonata,DNeasy (Qiagen) blood and tissue kit.,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5618.jpg,","
The control placing is switched compared to the protocol. For clarification the gel image is correctly labeled with the controls and my arthropod is labeled N, Control labels: W- = negative, W+ = Positive, H= water (-) control, D= DNA + Wolbachia DNA (+) Control, LD= DNA ladders, the other’s are my groupmates.<\/p>\n",No,GGGAAGAATGATTATATCGGATCTCATCCTCCTGCAGGATCAAAAAATGATGTATTAAAATTTCGGTCTGTAAGTAATAT TGTGATACCTCCTGCTAAAACTGGTAATGATAAAAAACAAAAAATAGTTGTCATGAAAATTGATCCAACGAATAAGGGGA TTTTGTCTGTTTTTATTGATGAAGGTTTTATTTTAATAGTTGAGGCTATAAAATTGATGCCCCCTAAAATTGAAAAAACT CCACCAAGGGGAAGGGAAAAAATTGCTAATTCTACGGAAGGGCCAGAGTGAGCGATATTTCTTGCTAATGGTGGGTAAAC TGTTCATCCAGTTCCAGCTCCTATTGATGTGAATGATCTGTAAAGACAAAGTGTGATTGATGGAGGCAATAGTCAAAATC TTATATTATTTATTCTTGGAAATGCTATGACGGGGGCTCTAAGTATTAAAGGAATTACCCAATTTCCGAATCCTCCAATT ATTATTGGTATTACTATAAATAAAATTATAATGAAGGGTGGGGATGTTATAATTGAGTTAAACATTTGCCCGATCAAAAT TAAACTTCCGGGTTGCCCAAATTCCAAAAAAATTAAGCTTCTAACGCTTGCTCCCTATATTCCTGAACATGCTCGAATAT AAAAACAATATCTTCATGTCTCGTATACATTGT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/nour-arthropod.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-15-at-14-05-58-IMG_3942.PNG-PNG-Image-1284-×-2778-pixels-—-Scaled-31.png,",41.7756400000,-88.0970000000,09/03/2024,"
Yellow beetle<\/p>\n",Chauliognathus pensylvanicus,DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/gel-electro.jpeg,","",No,AnAnGnnnnnnTnAAnTTTCnATCTGTnnnnnnnnTnnnnnnnnnnCnnGnnnnAACAGGTAATGAAnnnnnAAnGAnnA nGGnnnnnATTCCTACTGCTCACnnnAnTAAGGGGGTTTGAnCnAnAnnnnTnCnnnnAGnTCGTATATTAATTnTnnnT GAAATAAAATTAATTGCCCCTAAAATnnAnGAnnTTnnnGnTnnGnGTAAnGAnnnnAnGGCTAAATCTACnGATGCTnC TCCGnGAnnnATTAAnGAnnAnAATGGAGGnnnnnnnGTTCAACCnnnTCCTGCTCCnTTTTCTnCTATTCTTCTAnAnn nnAnnnnnnnnnnnGAnGGnGGAAnAnnCCnAAnTCTnnnnTnGnTTATTCnGnnnnnnnGnnnTATCAGGnnnTnnnnn nnnnnnnGnnnnTAnnnnnTTTCnnAnnnnnnnnnTnnnAnTGGnnATAAnnATAAAnAAAATnATnnnnnATGnnnGnn nnGnTnCnnTGACnTnATAAATTTGAnnnnnnnnnATAnnnnnnnCTGGnnGnnnnnnTTCnnnTCnnnnnnnnnnnCTn AnAnA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/70-A_AY1_Co1_R3.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_8320-1-scaled.jpg,",41.775639,-88.096949,09/03/2024,"
Collected by Fall 2024 students<\/p>\n",Formica pallidefulva,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_9288.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/85-B_AY1_Co1_R.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6223-e1745615230827.jpeg,",41.7784418000,-88.0952683000,09/03/2024,"
Collected by Fall 2024 students<\/p>\n
Medium-sized true bug.\u00a0 The body is elongate-oval and somewhat flattened. Color varies but is usually brownish to tan with some darker and lighter markings. Small head with long, thin antennae.<\/p>\n
<\/p>\n",Neurocolpus nubilus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Image-4-25-25-at-4.15 PM.jpeg,","",No,TGTTAATAGTATTGTNATGNNTCCTGCTAGAAN,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/NEW-82-B_AOR1_Co1_R-1-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/D3259BDA-6D84-450B-BB36-4B3C674E723A-2.jpg,",41.77564,-88.0970,09/03/2024,"
collected by fall 2024 students<\/p>\n",Chauliognathus pensylvanicus,DNeasy (Qiagen) blood and tissue kit.,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_1856-1-scaled.jpeg,","",No,>57-D_AG1_Co1_R.ab1 (581 bp) ATGATTGTATTTAAATTTCGATCTGTTAATAATATAGTAATAGCTCCTGCTAGTACTGGAAGTGATAATAATAATAATAA AGCAGTAATAACTACTGATCAAACAAATAAAGGTATACGATCTAATGAAATTCCTGTTGATCGTATATTAATTACAGTTG TAATAAAATTTACAGCTCCTAAAATTGAAGAAATTCCTGCTAAATGTAGAGAAAAAATAGCTAAATCAACTGAAGCTCCA CCATGAGCAATTCCAGCGGATAGAGGTGGATAAACAGTTCATCCTGTCCCAGCTCCATTTTCAACTATTCTACTTACAAA TAGTAAAGAAAGAGCAGGAGGTAGAATTCAAAATCTTATATTATTTATTCGTGGGAATGCTATATCAGGAGCACCTAATA TTAAAGGCACTAATCAATTTCCAAATCCACCAATTATAATAGGTATAACTATAAAAAAAATNNGCAGTTACAATTACATT ATAAATTTGATCATCTCCAATTAATGCTCCAGGATGTCCTAATTCAGCTCGAATTAAAATTCTTAAAGATGTTCCAACTA TTCCAGCTCAAGCTCCAAAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/57-D_AG1_Co1_R-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_8875-My-insect-scaled.jpeg,",41.7756400000,-88.0970000000,09/03/2025,"
Collected by Fall 2024 students<\/p>\n",Uroleucon leonardi,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Bands-for-DNA-and-Wolbachia.png,","
Controls were successful. My insect did not have Wolbachia but had DNA.<\/p>\n",No,AATGATAAAATTAATAAAATANCNGTAATTAAAATTGATCAAGGGAAAAGAGGAATTTGATTTAATTTTATATTATTTGG TATTATATTTATAATTGTACAAATAAAATTAATTGCTCCTAAAATTGATGAAATTCCTGCTAAATGTAAAGAGAAAATTG TTAAATCAACTGAAATATTATTATGTGCAATATTATTTGATAATGGTGGATAAATAGTTCATCCTGTTCCTGTTCCATTA TTAATTATAAAACTACAAATTATTATTATTAATGAAGGAGGTAATAATCAAAATCTAATATTATTTAAACGTGGAAATGA TATATCAGGACATCCTATTATTATAGGAATTAGTCAATTTCCAAAACCACCAATTACAATTGGTATAGTTATAAAAAAAA TTATAATAAAAGCATGAATTGTTACAATAACATTATATAATTGATTATTATTAATAATTGAATTAATTTGTCTTAATTCT AAACGAATTAAAATTCTAAGTGAAGATCCAATTATTCCTGATCAAATACCAAACAAAAAGTATAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/83-B_AOR2_Co1_R-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImageZTF1rP-scaled.jpg,",41.7756400000,-88.0970000000,09/03/2024,"
Collected by Fall 2024 students<\/p>\n",Ceratina calcarata,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/unnamed-1.png,","",Yes,ATCAAATAATAATATAGTAATAGCTCCTGCTAATACTGGTAATGATANAAGTAATAAAATTGCTGTAATAAATAC,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/84-B_AOR3_Co1_R-fasta.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_1038-1-scaled.jpg,",41.7756400000,-88.0970000000,09/03/2025,"",Sphex pensylvanicus,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/SectionATeam5.tif-1-scaled.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Insect_Common-names_ForwardSequence-1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6755.jpeg,",41.7756400000,-88.0970000000,09/03/2024,"
collected by Fall 2024 students<\/p>\n",Polites peckius,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6761.jpeg,","",No,>52-D_AY2_Co1_R.scf (626 bp) GCAGGATCAAAAAAAGAAGTATTTAAATTTCGATCTGTAAGTAGTATAGTAATAGCTCCTGCTAAAACAGGTAAAGATAAGAGTAACAATAAAGCTGTAATTCCTACAGATCAAACAAATAAAGGTATTTGGTCAAATGATAAATTTTTAATTCGTATATTAATGATTGTTGTAATAAAATTAATAGCTCCTAAAATTGAAGAGATTCCAGCTAAATGAAGAGAAAAAATTGCTAAATCAACAGAAGATCCTTGATGAGCAATATTAGAAGATAAAGGGGGGTAAACTGTTCAACCTGTTCCTGCACCATTTTCTACAATTCTTCTTGAAATTAATAATATTAATGAAGGAGGTAATATTCAAAAACTTATATTATTTATTCGAGGGAAAGCTATATCAGGAGCTCCTAGTATTAAAGGTACTAATCAATTTCCAAATCCTCCAATTATAATTGGTATAACTATAAAAAAAATTATAATAAAAGCATGAGCTGTAACAATAGTATTATAAATTTGATCATCTCCAATTAAAGATCCAGGATTACCTAATTCTGTTCGAATTAGTAAACTTAAGGAAGTTCCTAATATTCCTGCTCAAATACCAAAAATAAAATATAAAGTTCCAAT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3778.jpg,",41.7787078998,-88.0949008043,01/15/2025,"
Collected by Fall 2024 students<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-24-at-6.41.40-PM.png,","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/KassarAB4.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/20250205_133553-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/20250205_133542-2-scaled.jpg,",41.7756400000,-88.0970000000,09/03/2024,"
Collected by Fall 2024 students<\/p>\n",Halictus ligatus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-14-220258.png,","",Yes,AAAAATGATGTATTTAAATTTCGATCTGTTAATAATATTGTAATAGCTCCTGCTAAAACTGGAAGAGAGAGAAGAAGTAAAATTGCAGTAATTTTTACTGATCAAGGAAATAGAGGGATTTGATTTATATTAAGTGAAATATTTTTTATTAAAATAATAGTTACAATAAAATTAATAGCTCCTATGATTGAAGAAATTCCTGCTATATGTAAGGAGAAGATAGAAAAATCTACTGAAAAAGATGAATGAAATATAATTGATGATAATGGAGGGTAAATTGTTCATCCTGTTCCTGAACCTGAAGTTAAAGTATTTCCTATTAATAGTAGTAATAAGGAAGGAATTAATAATCAAAATCTTATATTATTTATTCGTGGGAAAGCTATATCTGGGGCCCCAATTATTAAGGGTACTAATCAATTTCCAAATCCTCCAATTATGAATGGTATAACTATAAAGAAAATTATAATAAAAGCATGGGCAGTAACAATAGTATTATAAATTTGATCATTATTAATTCATCTTCCAGGAGTTGCTAGTTCTATACGGATAATTATACTTAGTGATGCACCAATTATTCCTGATCATATAGCAAAAATAAAGTAAAGTATACCAATATCTTT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Nasir_92-B_AG1_Co1_R-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/FS1.jpeg,",41.7784418000,-88.0952683000,09/03/2024,"
collected by Fall 2024 students<\/p>\n",Lepidoptera,DNeasy (Qiagen) blood and tissue kit.,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-24-at-2.53.29-PM.png,","",No,>51-D_AY4_Co1_R.scf (642 bp) CTCCTCCTCCTGCTGGATCAAAAAAAGATGTATTTAAATTTCGATCAGTTAATAATATAGTAATAGCTCCAGCTAAAACA GGTAAAGATAATAATAATAATAATGCAGTAATACCTACAGATCATACAAATAATGGTATTTGATCAAATGATAAATTTTT AATTCGTATATTAATAATTGTTGTAATAAAATTAATAGCTCCTAAAATAGATGAAATTCCAGCTAAATGAAGGGAAAAAA TTGCTAAATCAACAGAAGATCCTTGATGAGCAATATTAGAAGATAGAGGGGGGTAAACTGTTCATCCTGTTCCTGCACCA TTTTCTACAATTCTTCTTGAAATTAATAAAGTTAATGAAGGGGGTAGTATTCAAAATCTTATATTATTTATTCGAGGAAA TGCTATATCAGGAGCTCCTAACATTAAAGGTACTAATCAATTACCAAATCCTCCAATTATAATTGGTATTACTATAAAGA AAATTATAATGAAAGCATGAGCTGTAACAATAGTATTATAAATTTGATCATCTCCAATTAAAGATCCAGGATTACCTAAT TCTGTTCGAATTAATAAACTTAAGGAAGTTCCTAATATTCCTGCTCAAATACCAAAAATAAAATATAAAGTTCCAATATC TT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/51-D_AY4_Co1_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_8524-1.png,",41.7784418000,-88.0952683000,,"
collected by Fall 2024 students<\/p>\n",Oncopeltus fasciatus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-24-at-12.31.11 PM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/MZs2025d-1-1-scaled.jpg,",41.7784418,-88.0952683,09/03/2024,"
collected by Fall 2024 students.<\/p>\n",Polistes dominula,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Gel-Electro-pic-Section-D-Group-4.jpg,","",Yes,>58-D_AG2_Co1_R.ab1 (641 bp) TCTCCTCCTGTAGGATCAAAAAAAGATGTATTTAAATTTCGATCAGTTAATAGTATAGTGATTGCTCCTGCTAAGACAGG TAAAGATAATAAAAGTAAAATTGCTGTAATTAAAACAGATCATGTAAATAAAGGTAAAAAATTTAATGAATGGGTTTTAA TATGTATATTTAAAATTGTTACAATAAAATTAATAGCTCCTATAATGGATGAGATTCCAGCAATATGAAGAGAAAAAATT GATAAATCAACAGAAGGTGAATTATGTCCAATATTTGAAGATAAAGGTGGGTATAAAGTTCATCCTGTTCCTACTCCTAT TTTTATTATGTTTCTTATAATAAGCAAAAATAATGAGGGTGGTAAAAGTCAAAATCTTATATTATTTATTCGTGGGAAAG CTATATCAGGGGCTCCAAGTATTATAGGAATTAATCAATTTCCAAAACCTCCAATTATAAATGGTATAACTATAAAAAAA ATTATTACTAAAGCATGGGCTGTAATAATTGAATTATAAAATTGATCATTATTAATAATTGAAGATGGTGTTCCTAATTC TAATCGAATTATTAATCTTAAAGATGTACCAATTATTCCAGCTCAAAGTCCAAAAATAAAATATAATGTTCCAATATCTT T,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/58-D_AG2_Co1_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-23-at-3.41.41 PM.png,",41.7756400000,-88.0970000000,09/03/2024,"
Collected by Fall 2024 students.<\/p>\n",Harmonia axyridis,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-23-at-3.44.00 PM.png,","",Yes,TGCTGTAATAAGAACAGATCATACAAATAAAGGAGTTTTATCAAGTATTATACCAAATGGACGTATATTTATAATAGTTG AAATGAAATTTACTGCACCTAAAATTGANGAAATTCCTGCTAAATGTAAACTANNNATCNCTAAATCTACTGAANGCCCA NTANGNGTTAAATTANAAGAAAGAGGAGGGNAAACAGTTCATCCTGTTCCTGCCCCTATTTCTACGATTGTTCTTAAAAT TAAAAGAGTTAAAGCAGGGGGTAAAAGTCAAAATCTTATGTTATTTAATCGTGGAAATGCTATATCAGGAGCTCCAATTA TTAAAGGAACTAATCAATTTCCAAAACCCCNAATTATAATAGGTATTACTATAAAGAAAATTATAATGAAAGCATGAGCT GTAACAATTATATTATAAATTTGNTCGTTTCCNATTAATCTTCCTCTAGTTCCNAATTCTAACCGAATTAAAATACTTAA CGATGTNCCTACTATTCCTGCCCNTATTC,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/aiza-arthropod-91-B_AP3_Co1_R.fa,, "",38.8189283028,-77.1735187291,,"",Aquarius remigis,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImage0nZXyZ-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImages6pxQz-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImage2Of2EH.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImage4Z2T2A-scaled.jpg,",47.4588686335,9.0181236087,03/05/2025,"
i found this insect 2 o’clock in the afternoon under leaves at the edge of a forest<\/p>\n",Isopoda,Instagene Matrix,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","
no bands on the gel<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0293.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0298.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0316-scaled.jpeg,",47.4588107680,9.0187098496,03/05/2025,"
I found this Pardosa amentata under some leaves near school. I collected it in the afternoon, about 2pm and it was sunny and about 54 degrees F outside.<\/p>\n",Pardosa amentata,Instagene Matrix,Partial abdomen,MiniPCR,TAE,SYBR Safe,"","
There were no bands on the Gel.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImagexI1UKR-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImageXHgJZC.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImageb27Sl9-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImageoBBFbg-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImagexYeDed-scaled.jpg,",47.4589159504,9.0189845869,03/05/2025,"
I found this insect at 2 o’clock in the afternoon under leaves at the edge of a forest<\/p>\n",Oniscus asellus,Instagene Matrix,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","
There were no bands on the gel.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0292-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0291.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0287.jpeg,",47.4593457296,9.0183287447,03/05/2025,"
$\"\"$ <\/p>\n
I found this Oniscus asellus under some tree bark near school. I collected it in the afternoon, about 2pm, it was sunny and about 54 degrees F outside.<\/p>\n",Oniscus asellus,Instagene Matrix,Partial abdomen,MiniPCR,TAE,SYBR Safe,"","
There were no bands.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-23-012555.png,",41.7756400000,-88.0970000000,09/03/2024,"
collected by fall 2024 students<\/p>\n",Parasteatoda tepidariorum,DNeasy (Qiagen) blood and tissue kit.,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-23-013116.png,","",Unknown,N/A,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/SAs2025d-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImageSL7hGz.jpg,",41.7756400000,-88.0970000000,09/03/2024,"
collected by Fall 2024 students<\/p>\n",Polistes fuscatus,DNeasy (Qiagen) blood and tissue kit.,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_1412.png,","",No,>56-D_AOR4_Co1_R.ab1 (588 bp) TATTAATATTTCGATCAGTTAATAGTATGGTAATTGCTCCAGCTAGAACAGGTAAAGATAATAAAAGTAAAATTGCAGTA ATTAAAACTGATCATGTAAATATAGGTAAAAAATTTAATGAATGAGTTTTAATGTGTATATTTAAGATTGTTACAATGAA ATTAATAGCTCCTATGATTGATGAAATTCCTGCAATGTGAAGAGAGAAAATAGCAAAATCAACTGATGATGAATTGTGAC CTATATTAGATGATAAAGGTGGATATAAAGTTCATCCAGTTCCTACTCCTATATCATTAATATTTCTAATAATTAATAAT AATAATGATGGTGGTAATAATCAAAATCTTATATTATTTATTCGTGGGAATGCTATATCTGGGGCACCTAGTATTAAGGG AATTAATCAATTTCCAAATCCTCCAATTATAAATGGTATAACTATGAAAAAAATTATAATTAATGCGTGAGCTGTAATAA TTGAATTATAGATTTGATCATTATTAATAATTGATAAAGGTGTACCTAATTCTAGACGAATAATTAATCTTAATGATCTT CCAATTATACCAGCTCATAGACCAAAGA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/56-D_AOR4_Co1_R_newer.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-22-at-10.41.42 PM-1.png,",41.7756400000,-88.0970000000,09/03/2024,"
collected by Fall 2024 students<\/p>\n",Boettcheria cimbicis,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-22-at-10.53.43 PM.png,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/93-B_AG2_Co1_Rnew.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/AImage-scaled.jpg,",41.7756400000,-88.0970000000,09/03/2024,"
Collected by Fall 2024 students<\/p>\n",Metylophorus novaescotiae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/95-B_AG4_Co1_R_LONGGGG.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/dragonflu-scaled.jpeg,",41.77564,-88.0970,09/03/2024,"
Collected by Fall 2024 students<\/p>\n",Erythemis simplicicollis,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/gel.jpeg,","",No,>68-A_AY3_Co1_R.scf (586 bp) GTATAGTAANNNNNNNNNNNNNNNACAGGNAGGNNNAAAAGTAATAATNNNNNNNGTANNNNNNNNNNNNNCATACAAAA AGAGGTATNNNNNCCANCTNNNTCCCAGGGGATTTTATATTGATTACNGTTGTAATAAAATTAATAGCTCCTAGAATGGA GGANACNCCTGCTAAGTGTAACGAGAAAATAGTTAAATCNACNNATGCTCCTGCATGTGCAATAGCTCCTGCCAATGGAG GATATACAGTTCATCCTGTTCCTGCTCCTCTTTCTACTATACTTCTAGATAAAAGTAATGTGAAGNNNNGAGGAAGTAAT CAAAAACTTATATTATTAAGACGGGGGAAGGCTATATCCGGGGCTCCTAATATTAAAGGAACTAACCAGTTTCCNAAGCC ACCAATTATAATAGGCATAACTATGNAGAANNTTATAACGAATGCGTGAGCAGTAACAATAACATTATAAATTTGATCAT CTCCNATTAAAGATCCTGGTTGGCCTAATTCNATTCNAATNNAAACTCTNAGAGCTGTTCNNATTATACCNGCCNNAGCA CCNAAGANTAAATACNAGGNTCCNAT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/68-A_AY3_Co1_Rupdated.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_1147-2-scaled.jpg,",41.8011410000,-88.0747875000,09/03/2024,"
collected by fall 2024 students<\/p>\n",Chrysopa oculata,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_2417-1-scaled.jpeg,",41.7754538000,-88.0965842000,09/03/2024,"",Vespula germanica,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-22-at-9.35.09 AM.png,","",No,>63-D_AB1_Co1_R (1).ab1 (671 bp) GGAAATGATGATATAAATTGGGTCCCCCCACCTGCAGGATCGAAAAAAGATGTGTTTAAGTTCCGATCTGTTAAAAGTAT AGTAATTGCTCCTGCAAGAACTGGTAGTGATAATAGAAGAAGAATTGCTGTAATTAAAATTGATCATGTGAATAAAGGAA GAAAATTTAATGAATGTGTTTTTGTGTGTATATTTAAAATAGTAACGATAAAATTAATTGATCCTATAATTGATGAAATT CCAGCAATATGAATTGAAAAAATTCCTAAATCTACAGATGGAGAATCATGACCAACAATAGAAGATAAAGGAGGGTATAA AGTTCATCCTGTTCCTACCCCTGTTCCAATAAAATTTCTTAAAATTAGTAAAAATAATGAAGGAGGGAGTAATCAAAATC TTATATTATTTATTCGAGGAAATGCTATATCAGGTACACCTAGTATTAAAGGGATTAATCAGTTTCCAAATCCTCCTACT AAAAAAGGTATAACTATAAAGAAAATTATAATAAAGGCATGAGCTGTAATAATTGTATTATAAATTTGATCATTATTAAT TAAGGCCCCAGGAGATCTTAATTCTAAGCGAATAATTATTCTTATTGAAGCTCCTAAAGTCCCTGCCCATAAAGCAAAAA TAAAATATAATGTTCCAATATCTTTATGTTT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Insect_Common-names_ReverseSequence-1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/SubbyArthropod1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/SubbyArthropod2.png,",41.7756400000,-88.0970000000,09/03/2024,"
collected by Fall 2024 students<\/p>\n",Parasteatoda tepidariorum,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/GelElectrophoresis.png,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/ArthropodSequenceProperForWPD.fa,, "",41.77564,-88.0970,09/03/2024,"
Collected by Fall 2024 students.<\/p>\n",Polistes fuscatus,DNeasy (Qiagen) blood and tissue kit.,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/image.jpeg,","",No,AGAAAGATGCTTGTCATTCTTAGTGATCTCCAACTCCTAATGGGTCAAAAAAAGATGTATTAATATTTCGATCAGTTAAT AGTATGGTAATTGCTCCAGCTAGAACAGGTAAAGATAATAAAAGTAAAATTGCAGTAATTAAAACTGATCATGTAAATAT AGGTAAAAAATTTAATGAATGAGTTTTAATATGTATATTTAAGATTGTTACAATGAAATTAATAGCTCCTATGATTGATG AAATTCCTGCAATGTGAAGAGAGAAAATAGCAAAATCAACTGATGATGAATTGTGACCTATATTAGATGATAAAGGTGGA TATAAAGTTCATCCAGTTCCTACTCCTATATCATTAATATTTCTAATAATTAATAATAATAATGATGGTGGTAATAATCA AAATCTTATATTATTTATTCGTGGGAATGCTATATCTGGGGCACCTAGTATTAAGGGAATTAATCAATTTCCAAATCCTC CAATTATAAATGGTATAACTATGAAAAAAATTATAATTAATGCGTGAGCTGTAATAATTGAATTATAGATTTGATCATTA TTAATAATTGATAAAGGTGTACCTAATTCTAGACGAATAATTAATCTTAATGATCTTCCAATTATACCAGCTCATAGACC AAAGATAAAATATAAAGTTCCAATATCTTTATGTTGGTGTTGAAAACA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6071-3-scaled.jpg,",41.7784418000,-88.0952683000,09/03/2024,"
Collected by Fall 2024 students.<\/p>\n",Eupeodes americanus,DNeasy (Qiagen) blood and tissue kit,body without wings,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-20-134950-edit.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Insect_Common-names_ForwardSequence.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/F4DA7FF0-D00C-4041-888E-ACBBE6C3D0DA.jpg,",41.7508391000,-88.1535352000,,"",Hymenoptera,Monarch DNA extraction (NEB),Abdomen,,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImageyJzEEc-scaled.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Insect-Screenshot.png,",41.7784418,-88.0952683,09/03/2024,"
Collected by Fall 2024 students<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit.,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-17-203310.png,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4913-scaled.jpeg,",41.77564,-88.0970,09/03/2024,"
collected by Fall 2024 students<\/p>\n",Polistes fuscatus,DNeasy (Qiagen) blood and tissue kit.,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5350-1-scaled.jpg,","",Yes,TTATATTTTNTCTTTGGTCTATGAGCTGGTATAATNGGAAGATCATTAAGATTAATTATTCGTCTAGAATTAGGTACACC TTTATCAATTATTAATAATGATCAAATCTATAATTCAATTATTACAGCTCANGCATTAATTATAATTTTTTTCATAGTTA TACCATTTATAATTGGAGGATTNGGAAATTGATTAATTCCCTTAATATTAGGTGCCCCAGATATAGCATTCCCACGAATA AATAATATAAGATTTTGATTATTACCACCATCATTAATATTATTAATTATTAGAAATATTAATGATATAGGAGTAGGAAC TGGATGAACTTTATATCCACCTTTATCATCTAATATAGGTCACAATTCATCATCAGTTGATTTTGCTATTTTCTCTCTTC ACATTGCAGGAATTTCATCAATTATAGGAGCTATTAATTTCATTGTAACAATCTTAAATATACATATTAAAACTCATTCA TTAAATTTTTTACCTATATTTACATGATCAGTTTTAATTACTGCAATTTTACTTTTATTATCTTTACCTGTTCTAGCNGG ANNNNNNTANNNNNNTATTAACTGATCGAAATANNAATNCNNNNNT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Insect_Common-names-1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3482-2.jpg,",41.77564,-88.0970,09/03/2024,"
collected by Fall 2024 students<\/p>\n",Oncopeltus fasciatus,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/50-D_AP1_Co1_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/arthropod--scaled.jpg,",41.7756400000,-88.0970000000,09/03/2024,"
collected by Fall 2024 students<\/p>\n",Formica pallidefulva,DNeasy (Qiagen) blood and tissue kit.,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-62.png,","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/86-B_AY2_Co1_R-2.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5174-scaled.jpeg,",41.7772658000,-88.0969165000,09/03/2024,"
Collected by Fall 2024 students<\/p>\n",Hylephila phyleus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",No,>46-D_AP3_Co_R.ab1 (608 bp)AAAAAGATGTATTTAAATTTCGATCAGTTAATAATATAGTAATAGCTCCAGCTAAAACAGGTAAAGATAATAATAATAATAATGCAGTAATACCTACAGATCATACAAATAATGGTATTTGATCAAATGATAAATTTTTAATTCGTATGTTAATAATTGTTGTAATAAAATTAATAGCTCCTAAAATAGATGAAATTCCAGCTAAATGAAGGGAAAAAATTGCTAAATCAACAGAAGATCCTTGATGAGCAATATTAGAAGATAGAGGGGGGTAAACTGTTCATCCTGTTCCTGCACCATTTTCTACAATTCTTCTTGAAATTAATAAAGTTAATGAAGGGGGTAGTATTCAAAATCTTATATTATTTATTCGAGGAAATGCTATATCAGGAGCTCCTAATATTAAAGGTACTAATCAATTACCAAATCCTCCAATTATAATTGGTATTACTATAAAGAAAATTATAATGAAAGCATGAGCTGTAACAATAGTATTATAAATTTGATCATCTCCAATTAAAGATCCAGGATTACCTAATTCTGTTCGAATTAATAAACTTAAGGAAGTTCCTAATATTCCTGCTCAAATACCAAAAATAAAATATAAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Alisha-Zehra_IMG_0016-scaled.jpg,",41.7784418000,-88.0952683000,09/03/2024,"
collected by Fall 2024 students<\/p>\n",Thomisus onustus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Picture1-1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_8772-scaled.jpeg,",41.7784418,-88.0952683,,"",,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5952-scaled.jpg,",31.9708128,-81.15865149999999,01/29/2025,"
<\/p>\n\n\n\n\n\n\n\n
Afternoon, 1 PM<\/span><\/td>\n<\/tr>\n
Dip net, Sweep net, <\/span>Digging by hand<\/span><\/td>\n<\/tr>\n
Dirt (dry and wet), fallen leaves & sticks, straw mulch, flower bed<\/span><\/td>\n<\/tr>\n
Georgia Southern Armstrong Campus, surrounding areas of student union<\/span><\/td>\n<\/tr>\n
Lady bug, ants, worm, and earwig<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n",Hymenoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_8092-scaled.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,GATCATCAATAAGAATAATTATTCGAATTGAATTAGgattctgTGGATCC ATTATTAATAATGACCAACTGTATAACTCTATCGTAACAGGACATGCATT TATCATAATTTTCTTTATAGTTATACCATTTATAATCGGAGGATTTGGTA ACTTTTTAATTCCACTAATAATTGGGTCACCAGATATAGCTTACCCCCGT ATAAATAACATAAGATTTTGACTCTTACCCCCATCAATTATACTACTCAC CTTAAGAAGATTTATTAATTCAGGTGCGGGAACTGGTTGAACTGTATACC CTCCCTTATCCTCAAGAATTTTCCATAGGGGAGCCTCAGTAGATTTAGCA ATTTTTTCTCTCCATATTGCAGGTATTTCATCAATCATAGGAGCTATCAA CTTCATCTCTACAATTATTAACATAACCCATAAAAATCTTTCAATAGATA AAACCCCTCTCATAGTATGATCTATTCTAATTACAGCAGTCCTTCTCCTC CTTTCCCTTCCTGTATTAGCCGGAGCAATTACAATACTACTAACAGATCG AAACCTTAATACTTCATTTTTTGACCCAGCAGGGGGAGGAGATCCTATTC TTTACCAACATTTATTTTGATTTTTTGGTCAC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4213-scaled.jpg,",40.7933949000,-77.8600012000,03/31/2025,"
This bug was found on a curtain for a glass door near the kitchen. It had been sunny outside and the bug was taken in the evening. The bug was alone and had been flying around the chandelier that was turned on.<\/p>\n",Halyomorpha halys,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4950-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4946-1-scaled.jpg,","
My arthropod was in well two in Gel 1. All of Gel 2 contained positive controls besides well seven. In Gel 1, wells one, four, and six were also positive while the others were negative.<\/p>\n",No,TNCAGCTATAAGATTAATTATCCGTATCGAATTAGGACAGCCTGGAAGATTTATTGGTAATGATCAAATTTATAATGTAA TTGTAACAGCACATGCATTTGTAATAATTTTCTTTATAGTAATACCAATCATAATTGGAGGATTCGGTAATTGATTAGTC CCTTTAATAATTGGAGCACCTGATATAGCCTTCCCACGATTAAATAATATAAGATTCTGATTATTACCCCCTTCATTAAC TTTATTATTAATAAGAAGAATAGCAGAATCAGGAGCTGGGACAGGATGAACAGTCTATCCCCCCTTATCAAGTAATATCT CACATAGAGGAGCATCAGTTGATTTAGCTATTTTTTCCTTACATTTGGCTGGGGTTTCATCAATTTTAGGGGCAGTAAAT TTTATTTCAACTATTATAAATATACGACCAACAGGTATAACCCCTGAACGAATCCCATTGTTTGTGTGATCAGTAGGAAT TACTGCACTTCTTCTGTTATTATCCTTACCTGTGTTAGCAGGTGCTATTACTATACTATTAACTGACCGAAATTTTAATA CCTCTTTTTTTGACCCATCAGGAGGA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/GE1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_1074-1-scaled.jpg,",41.77564,-88.0970,09/03/2024,"
Collected by Fall 2024 students.<\/p>\n",,DNeasy (Qiagen) blood and tissue kit.,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/insect_lable-Small-e1744939852349.jpeg,",31.9708128000,-81.1586515000,01/29/2025,"
\n
Habitat Description:<\/b>A large enclosed green house with high humidity and moisture.<\/li>\n
Collection Method:<\/b>Sweep nets, jars, and hand collection.<\/li>\n<\/ul>\n",Coleoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/gel_label.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,GTGGGAACATCCCTAAGAATACTAATTCGTACAGAATTaggcaatccTGG ATCTCTAATTGGAGACGACCAAATTTATAACGTactTgtAACAGCACATG CTTTTATTATAATTTTCTTTATAGTTATACCAATAATAATCGGGGGATTC GGAAATTGATTAGTCCCCCTCATACTAGGAGCACCTGACATAGCATTCCC ACGATTAAATAATATAAGATTCTGGCTTCTTCCACCATCATTAACTCTTC TTCTAATAAGAAGAATCATTGACAAAGGTGCAGGGACAGGTTGAACTGTT TACCCACCCTTATCAGCTAATGTGGCACATGAAGGACCTTCTGTAGACTT GGCTATTTTCAGACTCCATATAGCTGGTATTTCCTCAATTTTAGGGGCCA TTAATTTTATCTCTACAGTTATAAATATGCGACCTAAGGGTATAACCCTT GACCGTATACCACTTTTTGTATGAGCAGTTAAAATTACTGCTATTCTCCT TCTTCTATCCTTACCTGTATTAGCAGGAGCTATCACTATGCTACTAACAG ACCGTAATATCAACACTTCCTTCTTTGACCCCGCAGGAGGAGGTGATCCT ATCTTATACCAACACTTATTTTGATTTTTTGGTC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0021-scaled.jpeg,",41.7784418000,-88.0952683000,11/04/2030,"
collected by Fall 2024 students<\/p>\n",Harmonia axyridis,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0022.png,","
STEPS OF ISOLATION:<\/p>\n
\u2022Prepare sample<\/p>\n
\u2022Cell lysis and DNA precipitation<\/p>\n
\u2022DNA Purification<\/p>\n
DNA Elution<\/p>\n
STEPS OF PCR:<\/p>\n
\u2022PCR<\/p>\n
\u2022Purpose\/ Goal: The purpose of PCR is to amplify a specific DNA segment<\/p>\n
\u2022It is essential in identifying an organism and identifying infection<\/p>\n
\u2022Process:<\/p>\n
\u2022Denaturation<\/p>\n
\u2022DNA is heated which separates the double-stranded DNA into single strands by breaking hydrogen bonds<\/p>\n
\u2022Temperature: 94 \u2013 96 \u00b0C<\/p>\n
\u2022Annealing<\/p>\n
\u2022allow primers to bind (anneal) to their complementary sequences on the single-stranded DNA<\/p>\n
\u2022Temperature: 49-55 \u00b0C<\/p>\n
\u2022Elongation<\/p>\n
\u2022Synthesizes new DNA strands as Taq polymerase adds nucleotides to the primers<\/p>\n
Temperature: 72 \u00b0 C<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/18-C_AOR1_Co1_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/coleoptra-scaled.jpeg,",,,01/30/2025,"
head, thorax, abdomen, mandibles, 2 sets of wings, no pinchers<\/p>\n",Coleoptera,,head and lower body,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/gel-image-scaled.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,GTGGGAACCTCATTAAGAATACTTATTCGAGCTGAACTacgtattcCTGG ATCATTAATTGGAGATGACCAAATTTATAATGTAATTGTTACTGCTCATG CATTTATTATAATTTTTTTTATAGTTATACCAATTTTAATTGGGGGATTC GGAAATTGACTTGTACCTTTAATATTAGGAGCCCCTGATATGGCTTTCCC TCGAATAAATAATATAAGATTTTGATTACTCCCTCCATCACTGACTCTTT TACTGATAAGAAGCATAGTTGAAAGTGGGGCTGGTACAGGTTGAACTGTC TACCCCCCACTGTCATCAGGAATTGCTCATAGAGGAGCCTCAGTTGATTT AGCTATTTTTAGTCTTCACCTTGCAGGAGTTTCATCTATTCTAGGAGCTG TGAATTTTATTACAACAATTATTAATATACGTTCTACAGGAATAACATTT GAACGAATACCCCTATTTGTATGATCAGTAGGAATTACAGCTCTTTTACT TCTTTTATCTTTACCAGTATTGGCTGGAGCTATTACTATATTACTTACAG ATCGGAATTTAAATACCTCATTCTTTGACCCCGCAGGAGGAGGAGACCCT ATTTTATATCAACACTTATTCTGATTTTTTGGTCACCCTGAAGTT,,, "",41.77564,-88.0970,11/04/2030,"
collected by Fall 2024 students<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6463.jpg,",31.9708128,-81.15865149999999,01/30/2025,"",Hymenoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/5D7C66E5-2DC1-46EE-A589-D91AD5402400.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/zoomed-in-insect.jpg,",41.7756400000,-88.0970000000,09/03/2024,"
Collected by Fall 2024 students.<\/p>\n
Insect was killed with alcohol submersion.<\/p>\n
Insect was fairly deformed at the time of DNA extraction.<\/p>\n",Eupeodes americanus,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Team-3C-2-Gel-Electrophoresis-Wolbachia-Project-Alex-Hammad-2025-Spring.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/euop.-raw-data.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Alex-Krzus_IMG_0012-2-scaled.jpg,",41.7756400000,-88.0970000000,11/04/2030,"
Collected by Fall 2024 students<\/p>\n",Coenosia tigrina,DNeasy (Qiagen) blood and tissue kit,In front of University Building,Standard electrophoresis system,TAE,SYBR Safe,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Yasser-Samad_IMG_0013-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/AE86A551-37EC-411B-8653-3EAAC1E7B2C6-scaled.jpg,",,,11/04/2030,"
collected by Fall 2024 students<\/p>\n",Forficula auricularia,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/AE86A551-37EC-411B-8653-3EAAC1E7B2C6-scaled.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_8687-2.jpg,",,,01/30/2025,"
I collected my insect at Georgia Southern University, near the pond behind the Science Building. My collection site was an area of dead grass near the pond, and I used a sweep net to capture my insect.<\/p>\n",Hemiptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-17-at-4.10.10-PM.png,","
A DNA extraction kit of in-house reagents was used.<\/p>\n",No,ATAATTGgatAATATTAAGAATAATTATTCGAATTGAACTAGcactttca gCATCATTATTCTTTTTAGATAACAGCCACATTTATAACGTAATCGTTAC CTCTCACGCATTTATTATAATCTTCTTTATAGTCATACCAATTATAATTG GGGGATTTGGAAACTGACTTATCCCAATTATAATCGGGGCACCTGACATA GCATTTCCACGTCTAAATAATATAAGATTTTGACTTTTGCCACCATCTCT TACTTTACTACTAATAAGATCTATAATTGAAATAGGGGTAGGAACAGGCT GAACCGTATACCCACCATTATCAGGAAATATTACACATGCAGGGCCTAGT GTAGATATATCAATTTTCTCTCTACATTTAGCAGGAATCTCGTCAATTTT AGGTTCGATTAATTTTATCACTACAATCTTTAATATACGAACAGAAGGCA TAACAATACACCTTACACCGCTATTTGTATGATCTGTATTAATTACAGCC ATTCTTCTATTATTATCACTTCCAGTTCTTGCAGGAGCAATTACTATACT ATTAACAGACCGAAACCTGAATACAACATTCTTTGACCCATCGGGAGGGG GAGATCCAATTTTATACCAGCATTTATTTTGATTTTTTGGTC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Kamrin-Reniguntala_1_IMG_0020-scaled.jpg,",41.7756400000,-88.0970000000,09/03/2024,"
Collected by Fall 2024 Students.<\/p>\n",Syrphidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Team-2C-2.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/12-C_AP4_Co1_R-KR.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_1058-1-scaled.jpeg,",31.9708128000,-81.1586515000,01/30/2025,"
Collected near a pond; dead grassy area<\/p>\n
Collected with sweep net<\/p>\n",Hymenoptera,,savannah ga,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-17-155805.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,ATCATCTATAAGAATAATCATCCGATTAGAACTAGGATCTTGTAATTCTC TAATTAATAATGATCAAATCTACAACTCCTTAGTTACTAGACACGCTTTT ATTATAATTTTTTTTATAGTTATGCCCTTCATAATTGGAGGATTTGGAAA TTTCTTAGTGCCCTTAATACTTGGGTCCCCCGATATGGCCTATCCTCGAA TAAATAATATAAGATTTTGACTTCTGCCACCTTCTCTAACTCTTTTACTT ATCAGAAGATTTATTAACAGAGGAGTAGGAACAGGATGAACTATCTACCC ACCATTAGCCTCCAATATTTTTCACAGAGGGGCCTCTATTGATCTATCTA TTTTTTCCTTACATATCGCCGGAATATCATCAATTTTAGGAGCTATTAAT TTTATTTCTACAATCATTAACATACACCATAAAAATTTTACTATAGATAA AATCCCCCTACTAGTTTGATCTATCCTTATTACAGCCATTCTTCTTTTAC TTTCACTCCCAGTTCTTGCAGGAGCTATTACAATATTACTAACTGACCGT AATTTAAACACCTCCTTCTTCGATCCCTCAGGAGGAGGAGATCCCATTCT ATACCAACATTTATTCTGATTTTTTGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4043-scaled.jpeg,",31.979994535011787,-81.15811914876292,01/30/2025,"",Diptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4720-scaled.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,GTAGGAACATCTCTAAGAATTTTAATTCGAGCTGAATTAGGGCACCCAGG AGCTTTAATTGGAGATGATCAAATTTATAACGTAATTGTTACTGCACATG CTTTTGTAATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGATTT GGAAATTGACTAGTCCCATTAATATTAGGAGCACCTGATATAGCATTTCC TCGAATAAATAATATAAGTTTTTGATTACTTCCACCATCATTAACACTTT TAATGGCTAGAAGTATAGTGGAAAATGGAGCCGGAACAGGATGAACTGTA TATCCACCTTTATCTTCAATTATTGCACATGGGGGAGCTTCAGTAGATTT AGCTATTTTTTCTCTTCATTTAGCTGGGGTATCATCAATTTTAGGAGCAG TAAATTTTATTACTACAGTAATTAATATACGATCTACAGGAATTACATTC GATCGAATACCATTATTTGTATGATCAGTAGTAATTACTGCTTTATTATT ACTATTATCACTACCAGTATTAGCAGGAGCCATTACTATATTATTAACTG ATCGAAATTTAAATACTTCATTCTTTGATCCTGCAGGAGGAGGGGATCCA ATTTTATATCAACATTTATTTTGATTTTTTG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Image-4-16-25-at-3.55 PM.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Paula-C_IMG_0010-scaled.jpg,",41.7756400000,-88.0970000000,09/03/2024,"
collected by Fall 2024 students<\/p>\n",Drosophila melanogaster,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"","",No,AGGTAATCCAGCTGCAGATTCCCCTACTGCTACCTTGTTACGACTTCACCCCAGTCACCGATCCCACTTT AAATAACTCC CTCCTTGCGGTTAGGTCGTTAGCTTCGAGTGAAACCAATTCCCATGGCGTGACGGGCAGTGTGTACAAGA CCCGAGAACG TATTCACCGTGGCGTGCTAATCCACGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTACA ATCCGAACTG AGATGGCTTTTAAGGGATTAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTG TGTAGCCCAC TCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTTTCCTTAAA GTCCCCAGCA TTACCTGATGGTAACTAAGGATGAGGGTTGCGATCGTTGCGGGACTTAACCCAACATCTCACGACACGAG CTGACGACAG CCATGCAACACCTGTGTGAAACCCGGCCGAACCGACCCTATCCCTTCGAATAGGTATAATTTCCATGTCA AGGAGTGGTA AGGTTTTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTG AGTTTTAATC TTGCGACCGTAGTCCCCAGGCGGAATGTTTAACGCGTTAGCTGTAATACAGAAAGTAAAACTTCCCATAT TTAACATTCA TCGTTTACAGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGCGCCTCAGCGTCAG ATTTGAACCA GATAGACGCCTTCGCCACTGGTGTTCCTCCTAATATTTACGAATTTCACCTCTACACTAGGAATTCCTCT ATCCTCTTTC AATCTCTAGATTAGCAGTTTTAAAAGCAATTCCAAGGTTGAGCCTTGGGATTTCACTTTTAACTTACTAA TCCGCCTACG CACCCTTTACGCCCAATAATTCCGAATAACGCTAGCCCTCTCCGTATTACCGCGGCTGCTGGCACGGAGT TAGCCAGGAC TTCTTCTGTGAGTACCGTCATTATCTTCCTCACTAAAAGAGCTTTACAACCCAAAGGCCTTCTTCACTCA TGCGGCATGG CTGGATCAGGCTTTCGCCCATTGTCCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTATCT CAGTTCCAGT GTGGCTGATCATCCTCTCAGATCAGCTATAGATCATTGCCTTGGTAGGCTATTACTCCACCAACTAGCTA ATCTAATATA GGCTCATCTAATAGCAATAAATTTTTCCCCCGTAGGGCGTATACGGTATTAGTTGCCGTTTCCAACAATT ATTCCGTACT ACTAGGTAGATTCCTATACATTACTCACCCGTCTGCCACTAAGTTATACCATAGCAGGCTACAATATAAC TCCGTTCGAC TTGCATGTGTTAGGCCTGCCGCCAGCGTTCATTCTGAGCCAGGATCAAACTCT >A_wIrr (1505 bp) TAAGGAGGTAATCCAGCTGCAGATTCCCCTACTGCTACCTTGTTACGACTTCACCCCAGTCACCGATCCC ACTTTAAATA ACTCCCTCCTTGCGGTTAGGTCGTTAGCTTCGAGTGAAACCAATTCCCATGGCGTGACGGGCAGTGTGTA CAAGACCCGA GAACGTATTCACCGTGGCGTGCTGATCCACGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGA GTACAATCCG AACTGAGATGGCTTTTAAGGGATTAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGC ACGTGTGTAG CCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTTTCC TTAAAGTCCC CAGCATTACCTGATGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGAC ACGAGCTGAC CAGCATTACCTGATGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGAC ACGAGCTGAC GACAGCCATGCAACACCTGTGTGAAACCCGGCCGAACCGACCCTATCCCTTCGAATAGGTATAATTTCCA TGTCAAGGAG TGGTAAGGTTTTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTC CTTTGAGTTT TAATCTTGCGACCGTAGTCCCCAGGCGGAATGTTTAACGCGTTAGCTGTAATACAGAAAGTAAAACTTCC CATATTTAAC ATTCATCGTTTACAGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGCGCCTCAGC GTCAGATTTG AACCAGATAGACGCCTTCGCCACTGGTGTTCCTCCTAATATTTACGAATTTCACCTCTACACTAGGAATT CCTCTATCCT CTTTCAATCTCTAGATTAGCAGTTTTAAAAGCAATTCCAAGGTTGAGCCTTGGGATTTCACTTTTAACTT ACTAATCCGC CTACGCGCCCTTTACGCCCAATAATTCCGAATAACGCTAGCCCTCTCCGTATTACCGCGGCTGCTGGCAC GGAGTTAGCC AGGACTTCTTCTGTGAGTACCGTCATTATCTTCCTCACTAAAAGAGCTTTACAACCCAAAGGCCTTCTTC ACTCATGCGG CATGGCTGGATCAGGCTTTCGCCCATTGTCCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCG TATCTCAGTT CCAGTGTGGCTGATCATCCTCTCAGATCAGCTATAGATCATTGCCTTGGTAGGCTATTACTCCACCAACT AGCTAATCTA ATATAGGCTCATCTAATAGCAATAAATTTTTCCCCCGTAGGGCGTATACGGTATTAGTTGCCGTTTCCAA CAATTATTCC GTACTACTAGGTAGATTCCTATACATTACTCACCCGTCTGCCACTAAGTTATACCATAGCAAGCTACAAT ATAACTCCGT TCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCATTCTGAGCCAGGATCAAACTCTCAAATTT >A_wMel (1447 bp) CACCCCAGTCACCGATCCCACTTTAAATAACTCCCTCCTTGCGGTTAGGTCGTTAGCTTCGAGTGAAACC AATTCCCATG GCGTGACGGGCAGTGTGTACAAGACCCGAGAACGTATTCACCGTGGCGTGCTGATCCACGATTACTAGCG ATTCCAACTT CATGCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGGCTTTTAAGGGATTAGCTTAGCCTCGCGACT TTGCAGCCCA TTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCAC CTTCCTCCAG TTTATCACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCTGATGGTAACTAAGGATGAGGGTTGCGCTCG TTGCGGGACT TAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACCTGTGTGAAACCCGGACGAACCGAC CCTATCCCTT CGAATAGGTATAATTTCCATGTCAAGGAGTGGTAAGGTTTTTCGCGTTGCATCGAATTAAACCACATGCT CCACCGCTTG TGCGGGTCCCCGTCAATTCCTTTGAGTTTTAATCTTGCGACCGTAGTCCCCAGGCGGAATGTTTAACGCG TTAGCTGTAA TACAGAAAGTAAAACTTCCCATATTTAACATTCATCGTTTACAGCGTGGACTACCAGGGTATCTAATCCT GTTTGCTCCC CACGCCTTCGCGCCTCAGCGTCAGATTTGAACCAGATAGACGCCTTCGCCACTGGTGTTCCTCCTAATAT TTACGAATTT CACCTCTACACTAGGAATTCCTCTATCCTCTTTCAATCTCTAGATTAGCAGTTTTAAAAGCAATTCCAAG GTTGAGCCTT GGGA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-17-152829.png,",31.98078958644264,-81.15944717393894,01/30/2025,"
N\/A<\/p>\n",Orthoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_1483-scaled.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,N/A,,, "",32.0808989,-81.091203,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Picture1.jpg,",,,01/30/2025,"
mall insect, maroon brownish color<\/p>\n",Insecta,,Abdomen,Standard electrophoresis system,0.25 TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4428.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,ATTGGATCTTCAATAAGAATACTTATTCGTTTAGAATTaggtatttcaga tTCATTAATTAATAATGATCAAGTTTATAATTCTAtagtTACTAGACATG CTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTT GGAAATTTTTTAGTTCCTTTAATACTAGGATCCCCAGATATAGCCTATCC TCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATCAGTTTAC TTTTATTAAGAAATTTCATTAATGATGGAGTGGGAACAGGATGAACTGTT TACCCTCCATTAGCTTCTAATATTTTTCATAATGGCCCTTCAGTTGACTT AACAATTTTTTCTCTTCATATTGCGGGAATATCTTCTATTCTAGGAGCTA TCAATTTTATTTCAACAATTTTAAATATACGACATAAAAATTTTTCTTCT GATAAAATTCCTTTACTTGTTTGATCTATTTTAATTACAGCAATTTTACT TCTTCTTTCTTTACCAGTTCTTGCTGGAGCTATTACTATACTTTTAACTG ACCGAAATCTTAATACTTCCTTTTTTGATCCCTCAGGCGGAGGAGACCCT GTTCTTTATCAACATTTATTTTGATTTTTTGGTCA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0464.jpg,",31.979248859117053,-81.16100090389975,01/01/2025,"
caught with a sweep net in dry and dead matted grass field with an abundance of dead and rotted logs. Insect appeared as A green small insect with shell like outside<\/span><\/p>\n",,,head and lower body,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-17-at-3.33.38 PM.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,ATCTGATcaggAATAGTAGGAATAATATTAAGAATAATTATTCGAATTGA ACTAGCTcatccAGGACCATTTTTAACAAATGATCAATTATATAATGTAA TTGTAACATCACATGCATTTATTATAATTTTTTTCATAGTAATACCAATT ATAATTGGGGGGTTTGGTAACTGATTATTACCTCTAATAATTGGAGCACC AGATATAGCATTCCCACGAATAAATAATATAAGATTCTGACTTTTACCAC CATCACTAACACTTCTATTAGTTAGATCTATAGTAGAAATAGGAGCAGGA ACTGGTTGAACAGTATACCCACCATTATCATCTAATGCAGCACATTCAGG ACCTAGAGTTGACCTAGCAATTTTCTCACTACATTTAGCTGGTATTTCAT CAATCTTAGGAGCAGTTAATTTTATTACAACACTTATTAATATACGAAGT ATTGGTATAAATTTAGATCGAACACCATTATTTGCATGATCAGTTATAAT TACTGCAATTTTACTATTATTATCTCTACCAGTATTAGCTGGGGCAATTA CTATACTATTAACAGATCGTAATTTAAATACATCATTCTTTGACCCAGCT GGGGGTGGGGATCCTATTTTATATCAACATTTATTTTGATTTTTTGG,,, "",36.1444896,-86.8011462,01/30/2025,"
\n
$\"\"$ <\/li>\n<\/ul>\n",Blattodea,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/1000000089-scaled.jpg,","
DNA extraction kit of in-house reagents was used<\/p>\n",Yes,TTTTGgtgaTGATCCGGAATagtntggaaaTCATTAAGAATATTAATTCG TGCAGAATTaactcatcCTGGATCATTAATTGGAGATGATCAAATTTATA ATGTTATTGTAACAGCTCATGCCTTTGTTATAATTTTTTTTATAGTTATA CCTATTCTTATTGGAGGGTTTGGTAATTGATTAGTACCTTTAATACTAGG AGCTCCAGATATAGCATTCCCTCGAATAAATAATATAAGTTTTTGATTAC TTCCTCCTTCTTTAACTTTATTATTAGCGAGCAGTATAGTTGAAAGTGGT GCTGGAACAGGTTGAACTGTATACCCACCACTTGCAAGTGGTATTGCTCA TGCAGGAGCTTCTGTAGACTTAGCTATTTTCTCTTTACATTTAGCAGGTG TATCTTCAATTTTGGGAGCAGTTAACTTTATTTCAACAACAATTAATATA AAACCAATTAATATAAAGCCAGAACGAATTCCTTTATTTGTTTGAGCTGT AGGAATTACAGCATTATTGTTATTATTATCATTGCCTGTACTAGCAGGAG CAATTACAATATTACTTACAGATCGTAATTTAAATACTTCATTTTTTGAT CCCGCCGGTGGTGGAGATCCAATTCTTTATCAACATTTATTCTGATTTTT TGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0458-scaled.jpeg,",,,01/30/2025,"",,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/8440606457447903338.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-17-115750.png,",31.9708128,-81.15865149999999,01/30/2025,"
Black and red-orange insect by a tree.<\/p>\n",,,savannah ga,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-17-115009-1.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",Yes,TGATCAGGTATAGTTGGGTCATCCTTAAGATGAATTATTCGAATTGAACt ggccctgcgagGTTCATTTATTGGAGATGATCAAATTTATAATGtcatTG TAACTGCTCATGCATTTATTATAATTTTCTTTATAGTTATGCCAATTATA ATTGGAGGTTTTGGTAATTGGCTAGTACCTTTAATAATTGGTGCACCAGA TATAGCATTCCCCCGAATAAATAATATAAGTTTTTGGTTATTACCCCCAT CATTAACCCTACTATTAAGAAGTAGCATGGTTGAAATAGGGGCAGGAACA GGTTGAACTGTTTATCCTCCATTATCAAGCAATATTTCACATAGAGGGGC TTCAGTAGATTTAGCTATTTTTTCCCTCCATTTAGCAGGAGTTTCATCTA TTTTAGGAGCAATTAATTTTATCTCAACTATTATAAATATACGCCCAGCA GGTATAATTCCTGAGCGAACTCCTCTCTTTGTATGATCAGTGGGAATTAC TGCTCTTCTATTACTTTTATCCTTACCTGTTTTAGCAGGGGCTATCACTA TATTATTAACTGATCGTAATTTTAATACTTCCTTTTTTGACCCCACTGGT GGAGGAGACCCTATCCTTTATCAACACTTATTTTGATTTTTTGGTCAC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-10-at-11.22.23 AM.png,",,,01/16/2025,"",Dermaptera,,Abdomen,Standard electrophoresis system,TAE,Other,"","
DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_7256-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_7259-scaled.jpeg,",31.9789315,-81.1593385,01/30/2025,"
Collection Date: 1\/30\/2025<\/p>\n
Collection Time: 10:30 am<\/p>\n
Collection Method: Aerial\/Sweep Net<\/p>\n
Location of Collection: Georgia Southern University
\nArmstrong Campus \u2013 near
\npolice station and greenhouse<\/p>\n
Description of Habitat: Dry grass, wet mud under
\nlogs, small brush area with
\nleaves<\/p>\n",Blattodea,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_8264-scaled.jpeg,","
used DNA extraction kit of in-house reagents<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5901-scaled.jpeg,",,,01/30/2025,"
It was crazy and i did what i had to do for this class and found my insect quick but it was little insects outside.<\/p>\n",Hymenoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_2125-scaled.jpg,",31.9794892965,-81.1610655890,01/30/2025,"
01\/30\/2025
\n10:50 am
\nOutside the science building, near the university garden, Georgia Southern Armstrong campus
\nBushes, Trees, and Straw
\nSweeping net<\/p>\n",Blattodea,,Abdomen,Standard electrophoresis system,TAE,Other,"","
DNA extraction kit of in-house reagents was used<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/PicSample1.jpg,",,,01/30/2025,"
Specimen found in refuse area near the established collection site<\/p>\n",Insecta,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Electro1.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,TGGATAgtagGGACATCTTTAAGAATATTAATCCGAGCAGAACTAaatca tcCTGGTTCATTAATTGGAGATGATCAAATCTATAATGTTATTGTAACGG CTCACGCTTTTGTTATAATTTTCTTTATAGTTATGCCAATTTTAATTGGA GGATTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGC ATTTCCTCGTATAAATAATATAAGTTTTTGACTTTTACCTCCATCTCTAT CTCTTCTATTAGCCAGTAGCCTTGTCGAAAGAGGAGCTGGGACTGGTTGA ACCGTATACCCTCCCTTAGCTAGAGGAATTGCTCATGCTGGAGCTTCAGT AGATTTAGCTATTTTCTCATTACATCTTGCAGGTGTATCATCAATTTTAG GTGCCGTAAATTTTATTTCAACAATTATTAACATGAAACCAATTAATATA AGCCCTGAACGAATTCCTTTATTTGTTTGGTCAGTAGGTATTACTGCATT ATTATTATTATTATCCTTACCAGTTCTTGCAGGTGCAATTACAATATTAT TAACAGACCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGGGGAGGT GATCCTATTTTATATCAACATTTATTTTGATTTTTTGGTCACCC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6397-scaled.jpg,",31.9708128000,-81.1586515000,01/30/2025,"
01\/30\/2025
\n10:50 am
\nOutside the science building, near the university garden, Georgia Southern Armstrong campus
\nBushes, Trees, and Straw
\nSweeping net<\/p>\n",Insecta,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_7996-scaled.jpeg,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0991-1-scaled.jpg,",31.9708128000,-81.1586515000,01/30/2025,"
Collection Date<\/p>\n
1\/30\/2025<\/p>\n
Collection Time<\/p>\n
10:30 am<\/p>\n
Collection Method<\/p>\n
Aerial\/Sweep Net<\/p>\n
Location of Collection<\/p>\n
Georgia Southern University
\nArmstrong Campus \u2013 near
\npolice station and greenhouse<\/p>\n
Description of Habitat<\/p>\n
Dry grass, wet mud under
\nlogs, small brush area with
\nleaves<\/p>\n",Solenopsis,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/gel-2.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,ATCATCTATAAGAATAATCATCCGATTAGAACTAggctctTgtagtTCTC TAATTAATAATGATCAAATCTACAACTCCTTAGTTACTAGACACGCTTTT ATTATAATTTTTTTTATAGTTATGCCCTTCATAATTGGAGGATTTGGAAA TTTCTTAGTGCCCTTAATACTTGGGTCCCCCGATATGGCCTATCCTCGAA TAAATAATATAAGATTTTGACTTCTGCCACCTTCTCTAACTCTTTTACTT ATCAGAAGATTTATTAACAGAGGAGTAGGAACAGGATGAACTATCTACCC ACCATTAGCCTCCAATATTTTTCACAGAGGGGCCTCTATTGATCTATCTA TTTTTTCCTTACATATCGCCGGAATATCATCAATTTTAGGAGCTATTAAT TTTATTTCTACAATCATTAACATACACCATAAAAATTTTACTATAGATAA AATCCCCCTACTAGTTTGATCTATCCTTATTACAGCCATTCTTCTTTTAC TTTCACTCCCAGTTCTTGCAGGAGCTATTACAATATTACTAACTGACCGT AATTTAAACACCTCCTTCTTCGATCCCTCAGGAGGAGGAGATCCCATTCT ATACCAACATTTATTCTGATTTTTTGGTCA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0249.jpeg,",,,01/30/2025,"
In dirt caught with hands<\/p>\n",,,savannah ga,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_7750.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p><\/blockquote>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/unnamed-3-scaled.jpg,",,,01/30/2025,"
\n
Samples taken around grassy sides of roads<\/span><\/li>\n
Also in tall dry grass or under logs<\/span><\/li>\n
Using a water net samples were gathered<\/span><\/li>\n
Borrowed a hit stick<\/span><\/li>\n
Damp places where we were most lucky to find our samples<\/span><\/li>\n<\/ul>\n",,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/gel-1.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImageYECri1-scaled.jpg,",31.9708128000,-81.1586515000,01/30/2025,"\n\n<\/colgroup>\n\n\n\n\n\n\n
<\/p>\n
Collection Date<\/td>\n
<\/p>\n
1\/30\/2025<\/td>\n<\/tr>\n
<\/p>\n
Collection Time<\/td>\n
<\/p>\n
10:30 am<\/td>\n<\/tr>\n
<\/p>\n
Collection Method<\/td>\n
<\/p>\n
Aerial\/Sweep Net<\/td>\n<\/tr>\n
<\/p>\n
Location of Collection<\/td>\n
Georgia Southern University Armstrong Campus \u2013 near police station and greenhouse<\/td>\n<\/tr>\n
<\/p>\n
Description of Habitat<\/td>\n
Dry grass, wet mud under logs, small brush area with leaves<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n",Collembola,,Whole arthropod,Standard electrophoresis system,0.25 TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Image-4-17-25-at-11.24 AM.jpg,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,GAGTTTGATcGGCTATGATTGGTACTGCTTTTAGTGTATTAATTCGTTTA GAACttcgtcaaccGGGTAGTTTAATTGGTGATGATCAGATATACAATGT TTTAGTAACATCCCATGCATTTATTATGATTTTCTTTATGGTTATACCCA TCATAATTGGAGGTTTCGGTAACTGACTAGTACCTCTTATGATTGGAGCA CCTGATATGGCTTTTCCGCGGATAAATAATATAAGGTTTTGACTATTACC TCCCGCCCTTACTCTTTTATTAATAGGGGGTATAGTGGAAAGAGGTACAG GAACAGGATGAACTGTGTACCCCCCACTAGCTTCTTCTATCTCACACTCT GGGGCATCAGTAGATTTAACTATTTTTAGTCTTCATTTAGCTGGTGTTTC ATCTATTTTAGGTGCTATTAATTTTATTACAACAATTATTAATATACGAG CCCCAGGAATAAACTGAGATTTAACACCTCTTTTTGTGTGGTCTGTCTAT ATTACTGCAATTTTACTTCTCCTTTCACTACCAGTTTTAGCTGGTGCCAT TACAATACTTTTAACTGATCGAAATTTAAATACTACATTTTTTGATCCAG CTGGGGGGGGTGATCCTATTTTATATCAACATCTGTTTTGATTTTTTGGT CACCCTGAAG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_2029-scaled.jpeg,",31.9794001000,-81.1609070000,01/30/2025,"
\n
Collecting insects that may contain <\/span>Wolbachia<\/span><\/i><\/li>\n
Collected ants, lady bug, nats<\/span><\/li>\n
Gathered leaves, dry grass, dirt, rocks came with our samples<\/span><\/li>\n
Samples were taken at about 10:20 am – 10:40 am<\/span><\/li>\n
Samples taken around grassy sides of roads<\/span><\/li>\n
Also in tall dry grass or under logs<\/span><\/li>\n
Used a sweep net\u00a0<\/span><\/li>\n
Borrowed a hit stick<\/span><\/li>\n
Samples were found in damp shady places<\/span><\/li>\n<\/ul>\n",Collembola,,Whole arthropod,Standard electrophoresis system,0.25 TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screen-Shot-2025-04-17-at-11.23.47-AM.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,TTtGATcGGCTATGATTGGTACTGCTTTTAGTGTATTAATTCGTTTAGAA CTTGGtctacCGGGTAGTTTAATTGGTGATGATCAGATATACAATGTTTT AGTAACATCCCATGCATTTATTATGATTTTCTTTATGGTTATACCCATCA TAATTGGAGGTTTCGGTAACTGACTAGTACCTCTTATGATTGGAGCACCT GATATGGCTTTTCCGCGGATAAATAATATAAGGTTTTGACTATTACCTCC CGCCCTTACTCTTTTATTAATAGGGGGTATAGTGGAAAGAGGTACAGGAA CAGGATGAACTGTGTACCCCCCACTAGCTTCTTCTATCTCACACTCTGGG GCATCAGTAGATTTAACTATTTTTAGTCTTCATTTAGCTGGTGTTTCATC TATTTTAGGTGCTATTAATTTTATTACAACAATTATTAATATACGAGCCC CAGGAATAAACTGAGATTTAACACCTCTTTTTGTGTGGTCTGTCTATATT ACTGCAATTTTACTTCTCCTTTCACTACCAGTTTTAGCTGGTGCCATTAC AATACTTTTAACTGATCGAAATTTAAATACTACATTTTTTGATCCAGCTG GGGGGGGTGATCCTATTTTATATCAACATCTGTTTTGATTTTTTGGTCA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-02-08-at-11.28.34-AM.png,",,,01/30/2025,"
Used a beat stick and tweezers to obtain specimen<\/p>\n",Dermaptera,,Partial abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-17-at-10.57.49-AM.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/rachel-pic-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/rachel-pic-2-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/rachel-pic-3-scaled.jpg,",41.7754901000,-88.0965643000,09/03/2024,"
Caught by Fall 2024 students<\/p>\n",Formica fusca,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-17-at-12.40.10 AM.png,","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/phuwin-arthropod-9-C_AP1_Co1_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hover-Fly-Picture.png,",41.7756400000,-88.0970000000,09/03/2024,"
The sample was collected by Fall 2024 students.<\/p>\n",Eupeodes,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Gel-electrophoresis-Results-2.png,","",Yes,>A_AB2_Co1_R AATGATAAAAGTAATAATAAAGCAGTAATTACTACTGATCATACAAATAAAGGTATACGATCATAAGAAATTCCATTAGA TCGTATATTAATTACTGTAGTAATAAAATTTACAGCTCCTAAAATTGAAGATATACCAGCTAGATGAAGAGAAAAAATAG CTAAATCAACANAANCTCNNCCATGTGCAATACTAGCAGAAAGTGGAGGGTAAACTGTTCAACCAGTTCCAGCTCCATTT TCAACTATTCTTCTTACTAATAAAAGAGTTAAAGATGGGGGTAATAATCAAAAACTTATATTATTTATACGAGGAAAAGC TATATCAGGAGCTCCAAGTATTAAAGGAACTAATCAATTTCCAAATCCTCCAATTATAATAGGTATAACTATAAAAAAAA TTATAACAAAAGCATGAGCAGTTACAATTACATTATAAATTTGATCATCACCAATTAAAGCTCCTGGATGACCAAGTTCT GCACGAATTAATACACTTAAAGAAGTACCTACTATACCTGCTCAAGTACCAAATAAAAAATATAATG,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/A_AB2_Co1_R-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Sofia-Afreen_1_IMG_00221-scaled.jpg,",41.77564,-88.0970,09/03/2024,"
Collected by Fall 2024 students<\/p>\n",Polistes fuscatus,DNeasy (Qiagen) blood and tissue kit,body without wings,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Team-4C-2.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_9566.jpeg,",41.7756400000,-88.0970000000,09/03/2024,"
Collected by Fall 2024 students.<\/p>\n",Eupeodes luniger,DNeasy (Qiagen) blood and tissue kit.,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Team-2C.jpeg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/marthas-arthropod-fasta.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/94512E45-4899-432F-B257-E982D7D199B2-1.jpeg,",41.7756400000,-88.0970000000,09/03/2025,"
Collected by Fall 2024 students<\/p>\n",Phidippus,DNeasy (Qiagen) blood and tissue kit.,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6403-scaled.jpeg,",,,,"",Insecta,,next to the parking lot,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6629-scaled.jpg,","
DNA extraction kit of in-house reagent was used.<\/p>\n",No,,,, "",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/FullSizeRender-1.jpeg,",31.9708128000,-81.1586515000,01/29/2025,"
Time of Collection: 1:05pm – 1:50pm<\/p>\n
Habitat Collection Sites: Ant bed, tall dense bushes, moist & grassy dirt area<\/p>\n
Collection Method: Aerial Net & Beat Net<\/p>\n",Insecta,,head and lower body,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-16-at-2.57.48 PM.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,GAGCAGgaaTAATTGGATCATCTATAAGAATAATCATCCGATTAGAACTa gcatTtTgtgaTTCTCTAATTAATAATGATCAAATCTACAACTCCTTAGT TACTAGACACGCTTTTATTATAATTTTTTTTATAGTTATGCCCTTCATAA TTGGAGGATTTGGAAATTTCTTAGTGCCCTTAATACTTGGGTCCCCCGAT ATGGCCTATCCTCGAATAAATAATATAAGATTTTGACTTCTGCCACCTTC TCTAACTCTTTTACTTATCAGAAGATTTATTAACAGAGGAGTAGGAACAG GATGAACTATCTACCCACCATTAGCCTCCAATATTTTTCACAGAGGGGCC TCTATTGATCTATCTATTTTTTCCTTACATATCGCCGGAATATCATCAAT TTTAGGAGCTATTAATTTTATTTCTACAATCATTAACATACACCATAAAA ATTTTACTATAGATAAAATCCCCCTACTAGTTTGATCTATCCTTATTACA GCCATTCTTCTTTTACTTTCACTCCCAGTTCTTGCAGGAGCTATTACAAT ATTACTAACTGACCGTAATTTAAACACCTCCTTCTTCGATCCCTCAGGAG GAGGAGATCCCATTCTATACCAACATTTATTCTGATTTTTTGGTCACCCT GAAGTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Natalia-Zajac_IMG_0017-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-03-at-4.46.15 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0300.jpeg,",41.77564,-88.0970,09/03/2024,"
Collected by Fall 2024 students<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit.,body without wings,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Team-2C-1.jpg,","",No,>11-C_AP3_Co1_R.ab1 (610 bp) GTTTAAGTTTCGATCTGTTAAAAGTATAGTAATTGCTCCTGCAAGAACTGGTAGTGATAATAGAAGAAGAATTGCTGTAA TTAAAATTGATCATGTGAATAAAGGAAGAAAATTTAATGAATGTGTTTTTGTGTGTATATTTAAAATAGTAACGATAAAA TTAATTGATCCTATAATTGATGAAATTCCAGCAATATGAATTGAAAAAATTCCTAAATCTACAGATGGAGAATCATGACC AACAATAGAAGACAAAGGAGGGTATAAAGTTCATCCTGTTCCTACCCCTGTTCCAATAAAATTTCTTAAAATTAGTAAAA ATAATGAAGGAGGGAGTAATCAAAATCTTATATTATTTATTCGAGGAAATGCTATATCAGGTACACCTAGTATTAAAGGG ATTAATCAGTTTCCAAATCCTCCTACTAAAAAAGGTATAACTATAAAGAAAATTATAATAAAGGCATGAGCTGTAATAAT TGTATTATAAATTTGATCATTATTAATTAAGGCCCCAGGAGATCTTAATTCTAAGCGAATAATTATTCTTATTGAAGCTC CTAAAGTCCCTGCCCATAAAGCAAAAATAAAATATAATGTTCCAATATCT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/11-C_AP3_Co1_R-3.0.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/20250205_141732-scaled.jpg,",,,01/15/2025,"
Found in damp topsoil among leaves.<\/p>\n",Blattodea,,Partial abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4479.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,ATAGTAGGGACATCTTTAAGAATATTAATCCGAGCAGAACTaaccctatc tGgTTCATTAATTGGAGATGATCAAATCTATAATGTTATTGTAACGGCTC ACGCTTTTGTTATAATTTTCTTTATAGTTATGCCAATTTTAATTGGAGGA TTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGCATT TCCTCGTATAAATAATATAAgtTTTTGACTTTTACCTCCATCTCTATCTC TTCTATTAGCCagtaaccTTGTCGAAAGAGGAGCTGGGACTGGTTGAACC gTATACCCTCCCTTAGCTAGAGGAATTGCTCATGCTGGAGCTTCAGTAGA TTTAGCTATTTTCTCATTACATCTTGCAGGTGTATCATCAATTTTAGGTG CCGTAAATTTTATTTCAACAATTATTAACATGAAACCAATTAATATAAGC CCTGAACGAATTCCTTTATTTGTTTGGTCAGTAGGTATTACTGCATTATT ATTATTATTATCCTTACCAGTTCTTGCaggTGCAATTACAATATTATTAA CAGACCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGGGGAGgagAT CCTATTTTATATCAACATTTATTTTGATTTTTTGG,,, "",,,,"",,,,,,,"","",,,,, "",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_1546-1.jpg,",,,01/29/2025,"
collected tiny insect that from the moist dirt<\/p>\n
brown in color with orange stripes<\/p>\n",Collembola,pcr,Abdomen,Standard electrophoresis system,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4480-1.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-16-145747-1.png,",31.9708128000,-81.1586515000,01/01/2025,"
Method of collecting:\u00a0<\/span><\/p>\n
\n
Aerial\/Sweep net\u00a0<\/span><\/li>\n
Large specimen jar<\/span><\/li>\n
Gloved hands\u00a0<\/span><\/li>\n<\/ul>\n
Description of environment:<\/span><\/p>\n
A semi-dry landscape with moist, fertile soil, semi green grass and a flower near it.\u00a0<\/span><\/p>\n
<\/p>\n",Coleoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-7.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,GTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTaggcattagagG AAGATTAATTGGAAACGACCAAATTTATAATATAATTgtTACAGCTCATG CTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTTT GGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCC ACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTT TAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTT TACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTT AGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCAG TAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTaTAATACTT GATAAAACTCCTTTATTTGTATGATCTGTTCTTATTACAgCaatTCTTTT ATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACtatT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5962-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5963-scaled.jpeg,",,,01/29/2025,"
found in the dirt in a area that didnt have much sunshine<\/p>\n",Hemiptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-16-144151.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,CATCCATAAGTATTTTAATTCGAACTGAACTAggcctttcAGGATCACTA ATCGGAGATGATCAAATTTATAATGTAAtcttAACAGCTCATGCTTTTGT TATAATTTTCTTTATAGTTATACCTATTATAATTGGGGGATTTGGAAATT GATTAGTTCCTTTAATATTAGGAGCTCCTGATATGGCATTCCCTCGAATA AATAATATAAGATTTTGATTACTTCCCCCTTCTCTTACTATACTACTTGC TAGAAGTATAGTTGATATAGGTGCAGGAACTGGATGAACTGTTTATCCCC CCCTTTCTGCAGGAATTGCCCATGCTGGGTCATCTGTAGATATGGCTATT TTTTCATTACACCTTGCAGGAGTTTCCTCAATTCTAGGGGCTGTAAACTT TATTACCACAATAATTAATATACGTGCTCCAGGAATATCTCTTGATCAAA CACCTTTATTTGTATGAGCTGTAGGAATTACAGCTCTTCTTCTCCTCCTA TCTCTCCCCGTTCTGGCGGGAGCAATTACTATACTATTAACTGACCGAAA CTTAAATACTTCTTTCTTTGATCCTGCTGGAGGAGGAGATCCAATTCTTT ATCAACATTTATTTTGATTTTTTGGTCACCCTGGAAGTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImagehB2TUD-scaled.jpg,",,,01/29/2025,"
Caught in muddy, grassy, bushy, and tree environment.<\/p>\n",Orthoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImagescC4x4-scaled.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0128-scaled.jpg,",31.9708128000,-81.1586515000,01/29/2025,"
Our collection of method was using a sweep net, hands, and jar.<\/p>\n",Orthoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4160-scaled.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3871-scaled.jpeg,",,,01/29/2025,"
Collection Method: sweep net, hands, and jar<\/p>\n",Orthoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4482.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Insect_Drawing-scaled.jpeg,",31.9708128000,-81.1586515000,01/29/2025,"
Date: 01\/29\/2025
\nTime: 1:45 pm
\nLocation: Next to the Learning Commons
\nHabitat: Covered with leaves and in the dirt
\nMethod of Collection: By hand<\/p>\n",Scolopendromorpha,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Gel_Image.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,ASMAGTALSLIIRLELSQPGTLIGDDQTYNTIVTAHAFVMIFFMVMPIMIGGFGNWLTPL,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/bug-drawing-w-labels-.jpg,",31.9793350432,-81.1610959365,01/29/2025,"
The collection method I used was the Sweep Net and Dip Net.<\/p>\n
The habitat was very humid and mushy.<\/p>\n",,,head and lower body,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/gel.jpg,","
DNA extraction kit of in-house reagents was used<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_7839-3-scaled.jpg,",31.9708128000,-81.1586515000,,"",,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/68936.jpg,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0915-scaled.jpeg,",41.7756400000,-88.0970000000,09/03/2024,"
collected by Fall 2024 student<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Untitled-ocr.png,","",No,AGCATGGATGTTGATAGGATAGGGTCTCCTCCTCCCCTAGGGTCAAAAAAAGAGGTATTTAAATTTCGATCAGTTAATAATATAGTAATAGCTCCTGCTAATACAGGTAGGGATAAAAGTAAAAGTACAGCCGTTACTAATACTGATCAAACAAATAAAGGAACACGGTCTATTCTAATTCCAGCTGCTCGTATATTAATTATAGTAGTAATAAAATTCACAGCCCCTAAAATAGAAGAAGCTCCAGCAAGGTGGAGAGAAAAAATACCTAAATCTACAGATGCTCCTCTATGAGCAATTCTAGACGCCAGCGGAGGATATACTGTCCATCCTGTTCCTACTCCTCTTTCAACCAACCCACTTCTAAGCAATAAAGTTAAAGAAGGGGGTAAAAGTCAAAATCTTATATTATTTATTCGTGGAAAAGCCATATCTGGGGCTCCTAGTATTAATGGAATTAACCAATTACCAAATCCACCAATTATAATAGGTATTACTATAAAAAAAATTATTACAAAAGCATGAGCAGTTACAATTACATTGTAAATCTGGTCATCTCCAATTAAACTTCCAGGTTGTCCTAATTCAGTGCGAATAATTATTCTAAGGGCAGTTCCAACAGCCCCTGCTCAAACCCCAAATACAAAATATAAAGTTCCAATATCTTTATGTTTTGTTGGACCA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/88-B_AY4_Co1_R-fasta.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0022-scaled.jpeg,",36.1444896000,-86.8011462000,01/28/2025,"
\n
We used a dip net<\/li>\n
Gloved hands<\/li>\n<\/ul>\n",Blattodea,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0035.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,GCTTGATCAGGAATAGTAGGAACTTCACTTAGTATACTTATTCGAGCGGA ACTtcctttacgcggATCTCTTATTGGAGATGATCAAATCTATAATGTAA TTGTAACAGCACACGCCTTCATTATAATTTTCTTTATAGTAATACCCATT CTAATTGGTGGATTTGGAAATTGACTAGTTCCTCTTATATTAGGAGCACC AGATATAGCCTTTCCACGGATAAATAATATAAGATTCTGATTATTACCTC CTTCTTTGACCCTGCTATTAGCTAGAAGAATAGTAGAAAGTGGAGCTGGT ACTGGATGAACAGTCTACCCTCCACTTGCAAGAAGTATTGCACATGCTGG AGCATCTGTAGATCTTGCTATTTTTTCTCTACATCTCGCAGGAGTATCTT CAATTCTAGGAGCAGTAAACTTTATCTCAACAATTATTAATATAAAACCA ATTAGTATAAAACCTGAACGTATTCCTTTATTTGTATGAGCTGTTGGAAT TACTGCTTTACTTCTTCTTTTATCCTTACCAGTCCTTGCAGGGGCAATTA CTATACTTCTTACTGATCGAAATTTAAATACCTCCTTCTTTGACCCTGCT GGAGGAGGAGATCCAATTCTTTACCAACATTTATTTTGATTTTTTGGTCA CCCTGAAGTTTA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_2608-scaled.jpg,",31.9708128,-81.15865149999999,01/28/2025,"
Insect was caught on a tree on Georgia southern university campus.<\/p>\n",Hemiptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_2626.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,TGATCAGGTATAGTTGGGTCATCCTTAAGATGAATTATTCGAATTGAACT GGGCCAGCCAGGTTCATTTATTGGAGATGATCAAATTTATAATGTAATTG TAACTGCTCATGCATTTATTATAATTTTCTTTATAGTTATGCCAATTATA ATTGGAGGTTTTGGTAATTGGCTAGTACCTTTAATAATTGGTGCACCAGA TATAGCATTCCCCCGAATAAATAATATAAGTTTTTGGTTATTACCCCCAT CATTAACCCTACTATTAAGAAGTAGCATGGTTGAAATAGGGGCAGGAACA GGTTGAACTGTTTATCCTCCATTATCAAGCAATATTTCACATAGAGGGGC TTCAGTAGATTTAGCTATTTTTTCCCTCCATTTAGCAGGAGTTTCATCTA TTTTAGGAGCAATTAATTTTATCTCAACTATTATAAATATACGCCCAGCA GGTATAATTCCTGAGCGAACTCCTCTCTTTGTATGATCAGTGGGAATTAC TGCTCTTCTATTACTTTTATCCTTACCTGTTTTAGCAGGGGCTATCACTA TATTATTAACTGATCGTAATTTTAATACTTCCTTTTTTGACCCCACTGGT GGAGGAGACCCTATCCTTTATCAACACTTATTTTGATTTTTTGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_1106-scaled.jpeg,",31.9708128,-81.15865149999999,01/28/2025,"\n\n\n\n\n\n
Time of Collection:<\/b><\/td>\n 2:50pm<\/td>\n<\/tr>\n
Location of Collection:<\/b><\/td>\n in a pine tree near the library, shaded area.<\/td>\n<\/tr>\n
Description of Collection Site:<\/b><\/td>\n inside tree bark, dry wood, \u00a0at the base of the tree.<\/td>\n<\/tr>\n
Method of Collection: <\/b><\/td>\n Forceps and gloved hands<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n",Blattodea,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Image-4-2-25-at-9.57-PM-copy.jpg,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,GgGTCTCTGATTGGAGACGACCAGATCTACAACGTcatCGTTACCGCCCA CGCATTTGTTATAATCTTCTTTATGGTtagtccaccaaTATCtttaggAT ttgttgaCcaaCTAGTACCACTTATATTAGGAGCCCCAGATATGGCATTC CCACGAATAAACAACATAAGATTTTGATTACTCCCACCATCATTAACTCT ACTACTCACTAGTAGAACTGTAGAAAGTGGAGCAGGGACGGGATGAACAG TTTATCCCCCTCTTGCAAGAGGTATTGCCCATGCAGGAGCATCTGTAGAC TTAGCCATCTTCTCCCTACACCTAGCAGGTGTATCATCAATCCTAGGGGC AGTAAACTTTATCTCAACAACAATCAACATAAAACCAAAAAACATAAAAC CCGAACGAATCCCACTATTTGTATGATCTGTCGCCATCACGGCCCTACTC CTACTATTGTCCCTGCCAGTGTTGGCCGGAGCAATCACCATACTACTAAC CGACCGCAACCTAAATACATCATTCTTTGATCCAGCCGGAGGTGGAGATC CAATTCTATACCAACACTTATTCTGATTTTTTGGTCAC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6872-1-scaled.jpg,",,,,"",Hymenoptera,,In front of University Building,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-15-154607.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/lalala.jpeg,",31.9708128000,-81.1586515000,,"",Coleoptera,tweezers,,Standard electrophoresis system,lysis,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/lalala.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/20250204_154706-scaled.jpg,",,,01/28/2025,"
\n
Georgia Southern Armstrong Campus Hawes Hall<\/b><\/li>\n
Green leaves on the ground<\/b><\/li>\n
Heavy sunlight<\/b><\/li>\n
Dead Grass<\/b><\/li>\n
Sweep Net<\/b><\/li>\n
Green Leafhopper<\/strong><\/li>\n
Wings<\/strong><\/li>\n<\/ul>\n",Hemiptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/image_2025-04-15_153959038.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,ATTTTTGGATCTGATCaggaaTaGTAGGAATAATATTAAGAATAATTATT CGAATTGAACTAGctcatccAGGACCATTTTTAACAAATGATCAATTATA TAATGTAATTGTAACATCACATGCATTTATTATAATTTTTTTCATAGTAA TACCAATTATAATTGGGGGGTTTGGTAACTGATTATTACCTCTAATAATT GGAGCACCAGATATAGCATTCCCACGAATAAATAATATAAGATTCTGACT TTTACCACCATCACTAACACTTCTATTAGTTAGATCTATAGTAGAAATAG GAGCAGGAACTGGTTGAACAGTATACCCACCATTATCATCTAATGCAGCA CATTCAGGACCTAGAGTTGACCTAGCAATTTTCTCACTACATTTAGCTGG TATTTCATCAATCTTAGGAGCAGTTAATTTTATTACAACACTTATTAATA TACGAAGTATTGGTATAAATTTAGATCGAACACCATTATTTGCATGATCA GTTATAATTACTGCAATTTTACTATTATTATCTCTACCAGTATTAGCTGG GGCAATTACTATACTATTAACAGATCGTAATTTAAATACATCATTCTTTG ACCCAGCTGGGGGTGGGGATCCTATTTTATATCAACATTTATTTTGATTT TTTGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5100-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5099-scaled.jpeg,",,,01/28/2025,"
My bug has three legs and three sections to the bugs body. The bug has a head, thorax, and abdomen. This bug has been caught on campus at Armstrong Georgia Southern in Savannah, GA. This bug was captured on a leaf of a tree.<\/p>\n",Insecta,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-15-at-3.45.38-PM.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Unknown,GTAGGAACATCGTTAAGTATTTTaanttcGGTTAGAATTagcatttAGAG GAAGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCAT GCTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTT TGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTC CACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTT TTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGT TTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATT TAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCA GTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACT TGATAAAACTCCTTTATTTGTATGATCTGTTCTTATTACAGCAATTCTTT TATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTATTAACT GACCGAAACTTAAATTCTTCTTTTTTTGACCCAACCGGTGGGGGAGACCC AATTTTATACCAACATTTATTTTGATTTTTTGGTC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0047-scaled.jpeg,",,,01/08/2025,"
\n
abitat: Moist areas under debris<\/span><\/li>\n
Diet: Omnivorous diet\u00a0<\/span>\n
\n
Will even eat glue or fingernails<\/span>\u00b9<\/span><\/li>\n<\/ul>\n<\/li>\n
Common Names: Cockroaches\/Waterbugs<\/span><\/li>\n
Insect Life Stage: Nymph<\/span><\/li>\n
Unique trait: Cleanest bug in the wild<\/span>\u00b9<\/span><\/li>\n<\/ul>\n",Blattodea,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-15-3.43.38-PM.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_8607-scaled.jpeg,",41.775969847316965,-88.09561067762938,11/02/2030,"
collected by Fall 2024 students<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit.,Whole arthropod,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-15-at-2.39.05 PM.png,","",No,66-A_AP2_Co1_R (1).ab1 (598 bp) TGTATTTAAATTTCGATCAGTTAAAAGTATATGTAATAGCTCCTGCTAACACAGGAAGAGAAAGAAGAAGTAATACTGCA GTAATTAAAACAGCTCAAACAAATAAAGGTATTTGGTCTATTTTTATTTCTGAAGATCGTATATTTATAATTGTTGAAAT GAAATTTACAGCACCTAGAATTGATGAAATCCCTGCTATATGAAGTCTAAAAATAGATAAATCTACTGAAGATCCTCTAT GAGCAATATTAGAAGATAAAGGAGGGTATACTGTTCACCCAGTTCCAGTTCCATTTTCTACAATTCTTCTTATTAGTAAT AGAGATAATGATGGGGGTAAAAATCAGAATCTTATATTATTTATTCGTGGGAATGCTATATCAGGTGCTCCTAATATTAG TGGAACTAATCAATTTCCAAATCCACCAATTATGATTGGCATAACTATAAAGAAAATTATAATAAAAGCGTGTGCAGTTA CAACAACATTGTAAATTTGATCATTCCCAATTAGAGCATTTGTATTTCCTAATTCTGCTCGAATTAAAAGTCTTAGGGAT GTTCCTAGTATTCCTGACCATGCTCCAAATAAAAAATA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6360-scaled.jpeg,",,,,"
Collection Tray, picked up with hand.<\/span><\/p>\n
Ant hill in dry, dead, grass environment.<\/span><\/p>\n

<\/span><\/p>\n",Hymenoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-15-at-3.39.20 PM.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/20250121_150434-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-08-151140.png,",41.77564,-88.0970,09/03/2024,"",Melanoplus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-15-143206.png,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Ollies-Sequence-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Insect-Drawing.jpg,",,,01/28/2025,"
Cold day, sun out, grassy field<\/p>\n",Hymenoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Labeled-Gel-Image.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,TAATTGGATCATCTATAAGAATAATCATCCGATTAGAACTAGGATCTTGT AATTCTCTAATTAATAATGATCAAATCTACAACTCCTTAGTTACTAGACA CGCTTTTATTATAATTTTTTTTATAGTTATGCCCTTCATAATTGGAGGAT TTGGAAATTTCTTAGTGCCCTTAATACTTGGGTCCCCCGATATGGCCTAT CCTCGAATAAATAATATAAGATTTTGACTTCTGCCACCTTCTCTAACTCT TTTACTTATCAGAAGATTTATTAACAGAGGAGTAGGAACAGGATGAACTA TCTACCCACCATTAGCCTCCAATATTTTTCACAGAGGGGCCTCTATTGAT CTATCTATTTTTTCCTTACATATCGCCGGAATATCATCAATTTTAGGAGC TATTAATTTTATTTCTACAATCATTAACATACACCATAAAAATTTTACTA TAGATAAAATCCCCCTACTAGTTTGATCTATCCTTATTACAGCCATTCTT CTTTTACTTTCACTCCCAGTTCTTGCAGGAGCTATTACAATATTACTAAC TGACCGTAATTTAAACACCTCCTTCTTCGATCCCTCAGGAGGAGGAGATC CCATTCTATACCAACATTTATTCTGATTTTTTGGTC,,, "",41.7756400000,-88.0970000000,09/03/2024,"
collected by Fall 2024 students<\/p>\n",Drosophila melanogaster,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/77-A_AG4_Co1_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/268C20AA-77C7-440D-9832-58B190AA860C_1_201_a.jpeg,",31.97934,-81.161094,01/28/2025,"
cold day, sun out, grassy field<\/p>\n",Hymenoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/04/DakotaMadeline-scaled.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,CTAATTAATAATGATCAAATCTACAACTCctatatctttAtgatttgTTg accaaTAATTTTTTTTATAGTTATGCCCTTCATAATTGGAGGATTTGGAA ATTTCTTAGTGCCCTTAATACTTGGGTCCCCCGATATGGCCTATCCTCGA ATAAATAATATAAGATTTTGACTTCTGCCACCTTCTCTAACTCTTTTACT TATCAGAAGATTTATTAACAGAGGAGTAGGAACAGGATGAACTATCTACC CACCATTAGCCTCCAATATTTTTCACAGAGGGGCCTCTATTGATCTATCT ATTTTTTCCTTACATATCGCCGGAATATCATCAATTTTAGGAGCTATTAA TTTTATTTCTACAATCATTAACATACACCATAAAAATTTTACTATAGATA AAATCCCCCTACTAGTTTGATCTATCCTTATTACAGCCATTCTTCTTTTA CTTTCACTCCCAGTTCTTGCAGGAGCTATTACAATATTACTAACTGACCG TAATTTAAACACCTCCTTCTTCGATCCCTCAGGAGGAGGAGATCCCATTC TATACCAACATTTATTCTGATTTTTTGGTCACCCTGGAAGTTTA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0174-1-1-scaled.jpeg,",,,01/28/2025,"
insect with crab like pincher and the end of its body.<\/p>\n",Dermaptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-15-2.11.01-PM.png,",41.7756400000,-88.0970000000,09/03/2024,"
collected by Fall 2024 students<\/p>\n",Melanoplus differentialis,DNeasy (Qiagen) blood and tissue kit.,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-15-2.19.40-PM.png,","",No,CGGAAGGAACGTATCTGGTATTATTGATACCAACATCCTGCAGGATCAAAGAATGATGTATTTAAGTTTCGATCAGTTAATAATATTGTAATAGCTCCTGCTAGTACAGGAAGTGATAATAATAAAAGAAGTGCTGTAATAGCTACTGATCAAACAAATAATGGTGTTTGGTCTAATGTTATTCTTTCTGATCGTATATTGATTGCTGTTGTAATAAAATTAACTGCTCCTAGAATTGATGAAACACCAGCTAAGTGAAGAGAGAAAATGGCTAAATCAACTGATGCTCCTCCATGTGCAATTGCTCCAGCGAGCGGAGGGTAAACTGTTCATCCTGTACCAGCTCCTCTATCAATTATGGATGAAGGAAAGAAAAAAGGGTTAAGTGAGGGGGGAAAAAGTCAAAAACTTTTTTTTATTTTATTCGGGGGAAATGCTATTTCTGGTGCTCCAATTATTAATGGTACAAGTCAATTTCCAAATCCTCCAATTATAATAGGTATTACTATAAAGAAAATTATTACAAATGCGTGGGCTGTAATAATTACATTATAGATTTGGTCATCTCCAATTAAAGATCCTGGTTGACCTAATTCTGCTCGAATAATTATTCTTATAGAAGTTCCTACTATTCCAGCTCATGCTCCAAATATAAAGTATAAGGTACCAATATCTTTATGATTTGTTGACCA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/w9R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6983-1-rotated.jpg,",41.9069952,-87.687168,01/28/2025,"
$\"\"$ <\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit.,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6983-1-1-rotated.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-15-141938.png,","
$\"\"$ <\/p>\n",No,GAGGGTGATTGTTTCTCTATTTGTTCTCCTCCTCCTGCTGGATCACAAAATGATCGTATTAAGATTTCGATCAGTTAATAATATAGTAATTGCTCCTGCTAGTACTGGTAAAGATAACAATAAAAGAAATGCTGTAATTACTACTGATCAAACAAATAAAGGTATACGGTCTAAAGGAATTCCTGTAGATCGTATATTAATTACTGTAGTAATAAAATTTACTGCTCCTAAAATTGAAGATACTCCTGCCAGATGTAAAGAAAAAATAGCTAAATCAACCGAAGCACCTCCATGAGCGATTACTGATGATAAAGGTGGGTATACTGTTCATCCAGTACCGGCTCCGTTTTCTACTATTCTTCTTACTAATAGTAAGGTTAAAGCTGGTGGTAAAAGTCAAAATCTTATATTATTTATTCGTGGAAATGCTATATCAGGGGCTCCTAACATTAATGGAACTAATCAGTTTCCGAATCCACCAATTATAATAGGTATCACTATAAAAAAAATTATAATAAATGCATGTGCAGTTACAATTACATTGTAAATTTGGTCATCTCCAATTAGAGTTCCTGGGGCGTCCTAGTTCAGCACGGATTAGAATTCTTAAAGAAGTTCCTACTATTCCTGCTCAAGCTCCGAATATAAAATATAAAGTACCAATATCTTTATATT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/67-A_AP1_Co1_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_8179-scaled.jpeg,",41.7756400000,-88.0970000000,09/03/2024,"
Collected by Fall 2024 students.<\/p>\n",Orthoptera,DNeasy (Qiagen) blood and tissue kit,,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-15-at-2.11.48 PM.png,","",No,AAAAGAATGGTTGTTCAAGGAATCGGGATCTCCACCTCCAGCTTGGATCAAAGAATGATGTATTAATATTACGGTCTGTTAATAGTATTGTAATTTGCTCCAGCTAAAACTGGTAATGATAATAGTAATAGTAATGCTGTAATTGCTACTGATCAAACAAATAAGGGTGTTTGATCTATAGTTATTCTATTTGATCGTATATTAATTGCTGTTGTAATAAAGTTTACTGCTCCTAAAATTGATGAAATACCTGCTAAGTGTAATGAAAAAATAGCTAAATCTACTGATGCCCCACCATGTGCAATTGCTCCTGCTAATGGTGGGTAGACTGTCCATCCTGTACCTACCCCTCTATCAATTATTCTAGATGAGAGTAGTAAAATTAATGATGGTGGTAATAATCAAAATCTTATGTTATTTATTCGTGGAAATGCTATATCTGGTGCCCCAATTATTAATGGTACTAGCCAATTTCCAAAACCCCCAATCATAATTGGTATAACTATGAAGAAGATTATAACAAATGCATGTGCTGTGATAATAACATTATAAATTTGGTCATCTCCAATCATAGATCCTGGTTGCCCTAATTCAGCCCGAATAATTATTCTTATAGATGTTCCAACTATACCTGCTCATGCTCCAAATATAAAGTATAAAGTTCCAATATCTTTATGTTTTGTTGTGAACACACA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/74-A_AG1_Co1_R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-15-at-3.14.37 PM.png,",,,02/04/2025,"
Ant hill in dry, dead, grass environment.<\/span><\/p>\n
Collection Tray, picked up with hand.<\/span><\/p>\n

<\/span><\/p>\n

<\/p>\n",Hymenoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-15-at-3.19.42 PM.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/image0-1-scaled.jpeg,",,,04/15/2025,"",Dermaptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_7527.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_7394-scaled.jpg,",,,01/27/2025,"",Insecta,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_7366.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3460-1.jpeg,",,,01/28/2025,"
collection notes<\/p>\n
time: 10:00am-10:50AM<\/p>\n
Location: near gamble hall<\/p>\n
Habitat: under flower bush<\/p>\n
tools: sweep net<\/p>\n",Hymenoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3532-1-scaled.jpeg,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImagegrvfIh-scaled.jpg,",,,01/28/2025,"
\u2022Collection Time:\u00a010:00 -10:50<\/div>\n
\u2022Location:\u00a0Near Gamble Hall<\/div>\n
\u2022Habitat:\u00a0Near a flower bust<\/div>\n
.Collection Methods:<\/div>\n
Using sweep net<\/div>\n
<\/div>\n",Hymenoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Anh-man-hinh-2025-04-11-luc-2.20.01 SA.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0123-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0113-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0125-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0121-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0128-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0115-2-scaled.jpeg,",31.9891658,-81.1485513,01/28/2025,"
Collected 1\/28\/2025 at 10:28 AM.<\/p>\n
Next to the Department of Biology Greenhouse on the GSU Armstrong Campus.<\/span><\/p>\n
Cold, Wet, Muddy, Rocky collection site.<\/span><\/p>\n
Collected using forceps.<\/p>\n",Orthoptera,,Gonads dissected,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-1.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,GAGCAGGTATAGTTGGAACATCCATAAGTATTTTAATTCGAACTGAACTa gcactaCCAGGATCACTAATCGGAGATGATCAAATTTATAATGTAATCGT AACAGCTCATGCTTTTGTTATAATTTTCTTTATAGTTATACCTATTATAA TTGGGGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCTCCTGAT ATGGCATTCCCTCGAATAAATAATATAAGATTTTGATTACTTCCCCCTTC TCTTACTATACTACTTGCTAGAAGTATAGTTGATATAGGTGCAGGAACTG GGTGAACTGTTTATCCCCCCCTTTCTGCAGGAATTGCCCATGCTGGGTCA TCTGTAGATATGGCTATTTTTTCATTACACCTTGCAGGAGTTTCCTCAAT TCTAGGAGCTGTAAACTTTATTACCACAATAATTAATATACGTGCTCCAG GAATATCTCTTGATCAAACACCTTTATTTGTATGAGCTGTAGGAATTACA GCTCTTCTTCTCCTCCTATCTCTCCCCGTTCTGGCGGGAGCAATTACTAT ACTATTAACTGACCGAAACTTAAATACTTCTTTCTTTGATCCTGCTGGAG GAGGAGATCCAATTCTTTATCAACATTTATTTTGATTTTTTGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Doc-Feb-07-2025-09.03_01-scaled.jpg,",31.978757533616662,-81.16319890993097,01/28/2025,"
Big, Lots of legs, Color was dull<\/p>\n",Orthoptera,,Gonads dissected,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Doc-Apr-15-2025-11.14_01.jpg,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0717-scaled.jpeg,",31.9708128,-81.15865149999999,01/28/2025,"
Found on the base of a tree. Collected into a jar using hands.<\/p>\n",Lepidoptera,,head and lower body,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0749-scaled.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,GTTGGAACATCTTTAAGAATTTTAATTCGAGCTGAATTAGGACATCCTGG AGCATTAATTGGAGATGATCAAATTTATAATGTAATTGTAACTGCACATG CTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGTGGATTT GGAAATTGATTAGTGCCTTTAATATTAGGTGCTCCTGATATAGCATTCCC ACGAATAAATAATATAAGATTTTGACTTCTACCTCCTGCTCTTTCTTTAC TATTAGTAAGTAGAATAGTTGAAAATGGAGCTGGGACAGGATGAACTGTT TATCCACCTCTATCCGCTGGAATTGCTCATGGTGGAGCTTCAGTTGATTT AGCTATTTTTTCTCTACATTTAGCAGGAATTTCTTCAATTTTAGGAGCTG TAAATTTTATTACAACTGTAATTAATATACGATCAACAGGAATTTCATTA GATCGTATACCTTTATTTGTTTGATCAGTAGTTATTACTGCTTTATTATT ATTATTATCACTTCCAGTACTAGCAGGAGCTATTACTATATTATTAACAG ATCGAAATTTAAATACATCATTTTTTGACCCAGCGGGAGGAGGAGATCCT ATTTTATACCAACATTTATTTTGATTTTTTGGTCA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_9320-scaled.jpeg,",,,01/08/2025,"",Hymenoptera,,body without wings,Standard electrophoresis system,TAE,Other,"","
DNA extraction kit of in-house reagents was used<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_2375-rotated.jpg,",33.9892669,-84.440469,11/12/2024,"
The picture attached above was taken after death under a dissection scope, because no picture could be taken at the time of sample collection. It was found under a rock in Red Clay soil. It was identified using a Bug Guide as a Long Horned Bee under the genus Melissodes.<\/p>\n",Hymenoptera,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/7-arth-4.jpeg,","
When the gel was analyzed, there were no visible bands on the first lane, where the DNA Standard Ladder was inserted. So the gel was contaminated, or ran incorrectly, giving us completely inconclusive results. This gel was also running a reaction that tested for arthropod DNA, and a Wolbachia<\/em> test gel did not get run.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/HBs2025d-scaled.jpg,",41.77564,-88.0970,09/03/2024,"
collected by Fall 2024 students<\/p>\n",Apis mellifera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-15-at-8.38.38 AM.png,","
11-Electrophoresis<\/a><\/p>\n",No,>62-D_AB2_Co1_R.ab1 (605 bp) ATGTATTAAAATTTCGATCAAATAATAGTATAGTAATTCGCTCCAGCTAATACAGGTAATGATATAATTAATAAAATTGC TGTAATAAAAACTGATCATGGAAATAATGAAATTTGGTCATAATTTATAGAAAAATTTTTTATTATTATAATTGTAACTA TTAAGTTTAATGATCCTATAATTGAGGAAATTCCTGATATATGAAGAGAAAAAATTGCAAAATCTACTGAAGGTGAAGAA TGATATAAATATGCTGATAATGGTGGATATACTGTTCATCCAGTTCCTGGTCTTGGATAAAATAAATTTCTTAATAAAAG TATAAATAATGNGGGAGGAAGTAATCAAAATCTAATATTATTTATTCGGGGGAATGCTATATCAGGTGATCCTAGTATTA AAGGAATAAGCCAATTTCCAAATCCTCCAATTAAAAATGGTATAACTATAAAAAAAATTATTAGGAATGCATGACTAGTA ACAATTGTATTATAAATTTGATCATTGCTAATTCATGATCCTGGGGATCTTAATTCTATTCGAATAATAAGTCTCATTGA TGATCCTAGTATTCCAGATCATAAAGCTAGAATAATATACAAGAT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_8348-scaled.jpeg,",33.9890359690,-84.4382590381,11/12/2024,"
Lots of legs<\/p>\n
Found in the dirt<\/p>\n",Scolopocryptopidae,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/6-wol.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/6-arth.jpeg,","
gel electrophoresis was run and we got good results, sent for sequencing<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_8662-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_8670-scaled.jpeg,",33.9891238906,-84.4396520780,11/12/2024,"
They were moving very slow\/sluggish but they were surrounded by other insects along with their kind. They were found in the rotten log. For physical characteristics, they have a yellow- cream color, wingless, brown head\/mouth, six legs.<\/p>\n",Reticulitermes flavipes,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Capture-1-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Capture-1-2.png,","
PCR Procedure:<\/p>\n
\n
Remove all unnecessary items from your lab station<\/li>\n
\u00a0Put on nitrile gloves and clean all surfaces by wiping down with 70% Ethanol.<\/li>\n
\u00a0Collect a 1.5 mL microcentrifuge tube . Label the tube \u201cA\u201d<\/strong>\u00a0(for Arthropod PCR Cocktail). Place the tube in a 1.5mL tube rack.<\/li>\n
Collect six 0.2 mL PCR tubes . Number and label them with your initials. Place the tubes in a PCR tube rack and record putative identification of each arthropod in the table.<\/li>\n
\u00a0Use a P-2 or P-20 pipette to\u00a0add 2 \u03bcL of template DNA to each corresponding Arthropod PCR tube above. It is critical that you change tips between each tube.<\/li>\n
\u00a0Set aside the PCR tubes for now. Place the template DNA tubes back into storage. You will need these again for the Wolbachia\u00a0<\/em>PCR<\/li>\n
Use a P-200 pipette to add each of the following reagents\u00a0(from the \u201cTotal for 7 reactions\u201d column) to the 1.5 mL tube marked \u201cA\u201d. Change tips between each reagent, and check off each reagent after it is added.<\/li>\n
Vortex for 5 sec<\/li>\n
Place the A tube on one side of the mini-centrifuge and the B (balancer tube) on the opposite side. This is called \u201cbalancing\u201d the rotor; if the tubes are not balanced, it will make a loud sound and could damage the microcentrifuge.\u00a0Quickly spin down \u00a0(~ 3-5 seconds) the Arthropod PCR Cocktail to collect liquid at the bottom of the tube.<\/li>\n
Use a P-200 pipette to\u00a0add 23 \u00b5L of the Arthropod PCR Cocktail (A) to tubes 1-6 \u00a0(which should already contain the template DNA). Change tips between each tube. Never place a used pipette tip into the PCR Cocktail because it will contaminate all downstream reactions.<\/li>\n
Tightly secure the lids on each tube.<\/li>\n
Change the rotor \u00a0on your mini-centrifuge to the PCR tube rotor. Center tubes 1-3 on one side and 4-6 on the other side to balance the rotor.\u00a0Briefly spin \u00a0to collect all liquid at the bottom of the tubes.<\/li>\n
Transfer the tubes to the thermal cycler. Once everyone has placed their samples in the thermocycler, the program can be started \u00a0with the Arthropod PCR protocol. If using MiniOne, you may observe the PCR program as it cycles.<\/li>\n
Repeat all the steps for Wolbachia PCR<\/li>\n
Clean up your lab station and wipe surfaces with ethanol<\/li>\n
When the thermal cycler is done, store samples in the refrigerator (4\u00b0C).<\/li>\n<\/ol>\n
Gel Electrophoresis Protocol:<\/p>\n
\n
\u00a0Prepare the Gel with the DNA stain<\/li>\n
Once the gel is ready, place the combs into it and let it cool down<\/li>\n
Once the gel is cooled down and the wells are properly made within the gel, begin pipetting the samples<\/li>\n
Pipette 10uL of the DNA ladder in the first well. Hover your pipette above the well, and slowly empty your pipette<\/li>\n
Continue in this manner, carefully pipetting 10uL of each sample\/sample loading buffer mixture into separate wells in the gel. Change tips between each sample<\/li>\n
Place the lid on the gel box, connecting the electrodes appropriately<\/li>\n
Turn on the power supply to about 100 volts. Maximum allowed voltage will vary depending on size of the electrophoresis chamber<\/li>\n
Let the power run until the yellow (or bottom) band in the loading dye is \u00be down the gel. Then, turn off the power, disconnect the electrodes, and remove the lid and the gel using gloves.<\/li>\n
Place the gel on the transilluminator and note the presence\/absence of bands within each well.<\/li>\n<\/ol>\n",Unknown,My gel electrophoresis results did not show any visible bands for either arthropod DNA or Wolbachia DNA, indicating that my samples likely did not contain a successful arthropod sequence. The absence of arthropod DNA suggests that there may have been experimental errors during the process, which could include improper or incomplete DNA extraction, pipetting mistakes, degradation of the DNA samples, or errors during the PCR setup such as incorrect reagent concentrations or thermal cycling conditions. Because neither the arthropod nor Wolbachia DNA was amplified, I cannot confidently determine whether the arthropods I tested were truly free of Wolbachia, and the results are likely due to a procedural error rather than a biological absence.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_8360-scaled.jpeg,",33.9881827064,-84.4390011294,11/12/2024,"
Crawled a lot<\/p>\n
Reddish-brown<\/p>\n",Camponotus,DNeasy (Qiagen),body without wings,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/6-wol-1.jpeg,","
gel electrophoresis was run, the sample did not have any bands. The sample was not sent for sequencing.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_2133-scaled.jpeg,",41.7756400000,-88.0970000000,09/03/2025,"
Collected by Fall 2024 students<\/p>\n",Chauliognathus pensylvanicus,DNeasy (Qiagen) blood and tissue kit.,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImage1KIVt6.jpg,","
11-Electrophoresis<\/a><\/p>\n",No,>A1.ab1 (688 bp) ATATTGTTGGGGTATAAATTGGGTCTCCCCCACCGGTTGGGTCAAAAAAAGAAGAATTTAAGTTTCGGTCAGTTAATAGT ATAGTAATTGCTCCTGCAAGAACTGGTAGTGATAGTAATAAAAGAATTGCTGTAATAAGAACAGATCATACAAATAAAGG AGTTTTATCAAGTATTATACCAAATGGACGTATATTTATAATAGTTGAAATGAAATTTACTGCACCTAAAATTGAGGAAA TTCCTGCTAAATGTAAACTAAAAATCACTAAATCTACTGAAGGCCCATTATGTGTTAAATTAGAAGAAAGAGGAGGGTAA ACAGTTCATCCTGTTCCTGCCCCTATTTCTACGATTGTTCTTAAAATTAAAAGAGTTAAAGCAGGGGGTAAAAGTCAAAA TCTTATGTTATTTAATCGTGGAAATGCTATATCAGGAGCTCCAATTATTAAAGGAACTAATCAATTTCCAAAACCCCCAA TTATAATAGGTATTACTATAAAGAAAATTATAATGAAAGCATGAGCTGTAACAATTATATTATAAATTTGGTCGTTTCCA ATTAATCTTCCTCTAGTTCCTAATTCTAACCGAATTAAAATACTTAACGATGTTCCTACTATTCCTGCCCATATTCCAAA TAAAAAGTATAATGTTCCAATATCATTATGTTTGTTTGAAAACCAAAG,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/A1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/055C65B2-F8BC-4065-8F6C-A8DF9F3FA5F7_1_105_c.jpeg,",,,,"
Collected by digging in the dirt and leaves. Found in a damp cold environment.<\/p>\n",Dermaptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/83F9579F-129D-46E0-9FA7-6F7AEC8AF00A.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,TGGGGCTTGATCGGGgatgGTAGGAACTAATTTGAGAATTTTAATTCGAA CAGAGTtagcgttagcCTGGATCTTTAATTGGAGATGATCAGATTTATAA TGTTATTGTTACTGCTCACGCTTTTATTATAATTTTTTTTATGGTAATGC CAATTATAATTGGAGGGTTTGGTAATTGATTAGTTCCTTTAATATTGAGA GCTCCAGATATAGCTTTCCCACGAATGAATAATATAAGATTTTGGTTATT ACCTCCATCTCTGATTTTATTATTATCAGGGGGCCTAGTAGATAGAGGGG CTGGGACAGGTTGGACAGTGTATCCTCCTTTATCATCAGTGATTTCTCAT GGAGGGGCTTCAGTGGATATAACTATTTTTTCTTTACATTTGGCAGGGGT TTCTTCTATTTTGGGAGCCATTAATTTTATTACTACTGTGATTAATATAC GTCCAGAAGGTTTAAAGCTTGATCGTGTTCCTTTATTTGTTTGATCAGTA GCTATTACTGCTTTATTATTATTGCTTTCTTTACCGGTTTTAGCAGGAGC TATTACGATATTATTAACTGATCGAAATTTAAATACATCTTTTTTTGATC CAGCCGGAGGCGGAGATCCTATTTTATATCAACATTTATTTTGATTTTTT GGTC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/unnamed-1.jpg,",31.9708128,-81.15865149999999,01/28/2025,"
\n
Time of collection: 1:00-1:30<\/span><\/li>\n
location of collection: A pond and small stream<\/span><\/li>\n
Description of the environment: A pond and a small stream<\/span><\/li>\n
Method of collection: dip net and forceps<\/span><\/li>\n<\/ul>\n",,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/unnamed.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/bug-drawing-1.png,",31.9708128,-81.15865149999999,01/27/2025,"
elongated\/flat body, antennae, 3 pairs of “true” legs, mandibles.<\/p>\n",Dermaptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/9e59de8e-fb6c-453c-9c7c-4e647360cb83.png,","
DNA extraction kif of in-house reagents was used<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/InsectDrawing-scaled.jpg,",31.9708128000,-81.1586515000,01/27/2025,"
Insect was observed to have true legs, mandibles, beaded antennae, and no wings.\u00a0<\/span><\/p>\n",Insecta,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-14-161107.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,AATGGTCGGAACATCACTTAGAATACTAATTCGAACAGAACTAGGGctaC CTGGATCTCTAATTGGAGATGACCAGATCTACAACGTTATCGTTACTGCT CATGCATTCGTCATAATTTTCTTTATGGTCATACCAATCATGATTGGTGG ATTCGGAAACTGACTAGTACCACTTATATTAGGAGCCCCAGACATGGCAT TCCCACGAATAAACAACATAAGATTTTGATTACTCCCACCATCATTAACA TTACTACTTACTAGCAGAACAGTAGAAAGTGGAGCAGGGACAGGATGAAC AGTTTACCCCCCTCTTGCGAGAGGTATTGCCCATGCAGGAGCATCCGTAG ACTTAGCCATCTTCTCCTTACATCTAGCAGGTGTTTCATCAATTTTGGGA GCAGTAAATTTTATCTCAACAACAATTAACATAAAACCAAAAAATATAAA ACCCGAACGAATTCCACTATTCGTATGATCCGTCGCCATCACAGCTCTAC TCCTACTATTATCCCTGCCAGTACTAGCCGGAGCAATCACTATGTTATTA ACCGACCGCAACCTAAATACATCATTCTTTGACCCAGCTGGGGGTGGAGA CCCAATCCTATATCAACACTTATTCTGATTTTTTGGTCA,,, "",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3463-1-rotated.jpg,",31.9708128000,-81.1586515000,01/27/2025,"",Dermaptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5224-1.jpg,","
DNA extraction kit of in-house reagents was used<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImageUMaXaK-scaled.jpg,",,,01/27/2025,"
\n
01\/27\/2025<\/span><\/li>\n
2:15pm<\/span><\/li>\n
Outside of Science Center<\/span><\/li>\n
Parking lot<\/span><\/li>\n
Gloves<\/span><\/li>\n<\/ul>\n",Blattodea,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImagein5n1H-scaled.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,GTAGGAACTTCACTTAGTATACTTATTCGAGCAGAACTTAATctaCCCGG ATCTCTTATTGGGGATGATCAAATTTATAATGTTATTGTAACAGCACACG CCTTCATTATAATTTTCTTTATAGTAATACCCATCCTAATTGGTGGGTTT GGGAATTGGCTAGTTCCTCTTATATTAGGAGCACCAGATATAGCTTTTCC ACGGATAAATAATATAAGATTCTGATTATTACCTCCTTCTTTGACCTTGC TGCTGGCTAGAAGAATAGTAGAAAGTGGAGCTGGTACTGGATGAACAGTT TATCCTCCACTTGCAAGAAGTATTGCACATGCTGGAGCATCTGTAGATCT TGCTATTTTTTCTCTACACCTTGCAGGAGTATCTTCAATTCTAGGGGCAG TAAACTTTATCTCAACAATTATTAATATAAAACCAATCAGTATAAAACCT GAACGTATTCCTTTATTTGTATGAGCTGTTGGAATTACTGCTTTACTTCT TCTTTTATCCTTACCAGTCCTTGCAGGAGCAATTACTATACTTCTTACTG ATCGAAATTTAAATACCTCCTTCTTTGACCCTGCTGGAGGGGGAGACCCT ATTCTTTACCAACATTTATTTTGATTTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6582-scaled.jpeg,",,,01/19/2025,"
caught on a tree using a collection jar.<\/p>\n",Hymenoptera,,In front of University Building,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/FullSizeRender.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,ATAATTGGATCTTCAATAAGAATACTTATTCGTTTAGAATTAGGTACTTC AAATTCATTAATTAATAATGATCAAGTTTATAATTCTATAGTTACTAGAC ATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGA TTTGGAAATTTTTTAGTTCCTTTAATACTAGGATCCCCAGATATAGCCTA TCCTCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATCAGTT TACTTTTATTAAGAAATTTCATTAATGATGGAGTGGGAACAGGATGAACT GTTTACCCTCCATTAGCTTCTAATATTTTTCATAATGGCCCTTCAGTTGA CTTAACAATTTTTTCTCTTCATATTGCGGGAATATCTTCTATTCTAGGAG CTATCAATTTTATTTCAACAATTTTAAATATACGACATAAAAATTTTTCT TCTGATAAAATTCCTTTACTTGTTTGATCTATTTTAATTACAGCAATTTT ACTTCTTCTTTCTTTACCAGTTCTTGCTGGAGCTATTACTATACTTTTAA CTGACCGAAATCTTAATACTTCCTTTTTTGATCCCTCAGGCGGAGGAGAC CCTGTTCTTTATCAACATTTATTTTGATTTTTTGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4320-1-scaled.jpg,",31.9708128,-81.15865149999999,01/27/2025,"
Used bare hands to catch insect in moist soil.<\/p>\n",Insecta,,,,,,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6945-scaled.jpeg,",,,01/27/2025,"
\u2022 Insect collected on 1\/27\/25 at 2:00:00 PM
\n\u2022 Georgia Southern University (Armstrong Campus)
\n\u2022 Used swift force on trees nearby body of water
\n\u2022 Gloved hands used to break open trees near pond
\n\u2022 Insect found within branches using gloved hand method<\/p>\n",Blattodea,,,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-14-3.19.23-PM.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,TGGATAGTAGGGACATCTTTAAGAATATTAATCCGAGCAGAACTAAATCA ACCTGGTTCATTAATTGGAGATGATCAAATCTATAATGTTATTGTAACGG CTCACGCTTTTGTTATAATTTTCTTTATAGTTATGCCAATTTTAATTGGA GGATTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGC ATTTCCTCGTATAAATAATATAAGTTTTTGACTTTTACCTCCATCTCTAT CTCTTCTATTAGCCAGTAGCCTTGTCGAAAGAGGAGCTGGGACTGGTTGA ACCGTATACCCTCCCTTAGCTAGAGGAATTGCTCATGCTGGAGCTTCAGT AGATTTAGCTATTTTCTCATTACATCTTGCAGGTGTATCATCAATTTTAG GTGCCGTAAATTTTATTTCAACAATTATTAACATGAAACCAATTAATATA AGCCCTGAACGAATTCCTTTATTTGTTTGGTCAGTAGGTATTACTGCATT ATTATTATTATTATCCTTACCAGTTCTTGCAGGTGCAATTACAATATTAT TAACAGACCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGGGGAGGT GATCCTATTTTATATCAACATTTATTTTGATTTTTTGGTCA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG-4189-1-scaled.jpg,",,,01/21/2025,"
\n
Cold ground; cloudy and rainy weather<\/span><\/li>\n
Small shrubs and tall, covered moss tree\u2019s; cluttered piles of leaves & twig on the ground<\/span><\/li>\n
Abandoned field with tree trunks covered in moss and vines by piles of sand and dirt<\/span><\/li>\n<\/ul>\n",Dermaptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG-7776-1-scaled.jpg,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6665-1-1-scaled.jpg,",,,01/27/2025,"
Collected GSU Armstrong campus<\/p>\n
Underneath trees, Shaded, near body of water<\/p>\n
Used swift force<\/p>\n
Gloved Hand method<\/p>\n",Blattodea,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6946-scaled.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n
<\/p>\n",No,TATCTTCggagCTTGATCTGGaATAGTAGGGACATCTTTAAGAATATTAA TCCGAGCAGAACTAAAtctaccTGGTTCATTAATTGGAGATGATCAAATC TATAATGTTATTGTAACGGCTCACGCTTTTGTTATAATTTTCTTTATAGT TATGCCAATTTTAATTGGAGGATTTGGAAATTGATTAGTACCTTTAATAT TAGGTGCTCCTGATATAGCATTTCCTCGTATAAATAATATAAGTTTTTGA CTTTTACCTCCATCTCTATCTCTTCTATTAGCCAGTAGCCTTGTCGAAAG AGGAGCTGGGACTGGTTGAACCGTATACCCTCCCTTAGCTAGAGGAATTG CTCATGCTGGAGCTTCAGTAGATTTAGCTATTTTCTCATTACATCTTGCA GGTGTATCATCAATTTTAGGTGCCGTAAATTTTATTTCAACAATTATTAA CATGAAACCAATTAATATAAGTCCTGAACGAATTCCTTTATTTGTTTGGT CAGTAGGTATTACTGCATTATTATTATTATTATCCTTACCAGTTCTTGCA GGTGCAATTACAATATTATTAACAGACCGAAATTTAAATACTTCATTTTT TGACCCTGCAGGGGGAGGTGATCCTATTTTATATCAACATTTATTTTGAT TTTTTGGTCACC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-14-150943.png,",31.9708128,-81.15865149999999,06/06/2025,"
Cold ground; cloudy and rainy weather
\nSmall shrubs and tall, covered moss tree\u2019s; cluttered piles of leaves & twig on the ground
\nAbandoned field with tree trunks covered in moss and vines by piles of sand and dirt<\/p>\n
collected with gloved hands<\/p>\n",Blattodea,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-09-180633.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,TAGTAGGGACATCTTTAAGAATATTAatctcgaGCAGAACTAAATctaCC TGGTTCATTAATTGGAGATGATCAAATCTATAATGTTATTGTAACGGCTC ACGCTTTTGTTATAATTTTCTTTATAGTTATGCCAATTTTAATTGGAGGA TTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGCATT TCCTCGTATAAATAATATAAGTTTTTGACTTTTACCTCCATCTCTATCTC TTCTATTAGCCAGTAGCCTTGTCGAAAGAGGAGCTGGGACTGGTTGAACC GTATACCCTCCCTTAGCTAGAGGAATTGCTCATGCTGGAGCTTCAGTAGA TTTAGCTATTTTCTCATTACATCTTGCAGGTGTATCATCAATTTTAGGTG CCGTAAATTTTATTTCAACAATTATTAACATGAAACCAATTAATATAAGC CCTGAACGAATTCCTTTATTTGTTTGGTCAGTAGGTATTACTGCATTATT ATTATTATTATCCTTACCAGTTCTTGCAGGTGCAATTACAATATTATTAA CAGACCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGGGGAGGTGAT CCTATTTTATATCAACATTTATTTTGATTTTTTGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/diptera.png,",31.9708128,-81.15865149999999,01/27/2025,"",Insecta,,Whole arthropod,,0.25 TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/gel-1.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/unnamed.jpg,",,,01/22/2025,"",Hymenoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-14-141127.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",Yes,CGGCTCCTCAATAAGAATAATTATTCGACTTGAACTTgcttctTGTAATT CTTTAATTAATAATGATCAAATTTATAATACTTTAGTAACCAGACATGCT TTTATTATAATTTTTTTTATAGTAATACCCTTCATAATTGGAGGATTTGG AAATTTTCTAATTCCTTTAATACTTGGATCCCCAGATATAGCCTACCCTC GTATAAATAATATAAGATTCTGACTTCTTCCTCCCTCTCTCTCCCTCCTT CTTATAAGTAGATTTATTAATACAGGAGTTGGTACAGGTTGAACAATTTA CCCACCTTTAGCCTCTAATACCTTTCATAGTGGACCTTCTATTGACCTAT CCATTTTTTCCCTCCATATTGCTGGAATATCATCTATTCTAGGTGCTATC AATTTTATTGCTACAATTATTAATATACACCACCAAAATTTTTCTATAGA TAAAATCCCCTTATTAGTCTGATCTATTTTAATTACTGCCGTACTTTTAC TTCTTTCCCTTCCTGTTCTAGCAGGCGCAATCACTATACTTCTAACAGAT CGAAATTTAAATACATCCTTTTTTGATCCAGCAGGAGGAGGTGACCCAAT TCTTTATCAACACTTATTTTGATTTTTTGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/tempImageCRUIAt-scaled.jpg,",,,,"
found in<\/p>\n
Roughly 4 inches into soft dirt and mulch, Icy ground, cloudy skies, roughly 45 degrees fahrenheit. Surrounded by trees, bushes and other ground vegetation.<\/span><\/p>\n

<\/p>\n",,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-14-at-1.57.51 PM.png,","
“DNA extraction kit of in-house reagents was used.<\/p>\n",No,CTTGATCGGGgatgGTAGGAACTAATTTGAGAATTTTAATTCGAACAGAG TTagcgtcagCCTGGATCTTTAATTGGAGATGATCAGATTTATAATGTTA TTGTTACTGCTCACGCTTTTATTATAATTTTTTTTATGGTAATGCCAATT ATAATTGGAGGGTTTGGTAATTGATTAGTTCCTTTAATATTGAGAGCTCC AGATATAGCTTTCCCACGAATGAATAATATAAGATTTTGGTTATTACCTC CATCTCTGATTTTATTATTATCAGGGGGCCTAGTAGATAGAGGGGCTGGG ACAGGTTGGACAGTGTATCCTCCTTTATCATCAGTGATTTCTCATGGAGG GGCTTCAGTGGATATAACTATTTTTTCTTTACATTTGGCAGGGGTTTCTT CTATTTTGGGAGCCATTAATTTTATTACTACTGTGATTAATATACGTCCA GAAGGTTTAAAGCTTGATCGTGTTCCTTTATTTGTTTGATCAGTAGCTAT TACTGCTTTATTATTATTGCTTTCTTTACCGGTTTTAGCAGGAGCTATT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/hymenotera-drawing-.jpg,",,,01/27/2025,"
\n
Insects collected on January 27th, 2025.\u00a0<\/span><\/li>\n
Collected at 1:15pm EST<\/span><\/li>\n
Insects were collected at the courtyards near the Science center on the Georgia Southern Armstrong campus.\u00a0<\/span><\/li>\n
The insects collection site included rotting branches, small amounts of snow, pine straw and leaves.\u00a0<\/span><\/li>\n
The insects were collected through a method of sorting through dirt with the soft tweezers.\u00a0<\/span><\/li>\n<\/ul>\n",Hymenoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Copy-of-Module-8-part-2.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,GGATCTTCaaTAAGAATACTTATTCGTTTAGAATTacgtatttcAAATTC ATTAATTAATAATGATCAAGTTTATAATTCTATAGTTACTAGACATGCTT TTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGA AATTTTTTAGTTCCTTTAATACTAGGATCCCCAGATATAGCCTATCCTCG AATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATCAGTTTACTTT TATTAAGAAATTTCATTAATGATGGAGTGGGAACAGGATGAACTGTTTAC CCTCCATTAGCTTCTAATATTTTTCATAATGGCCCTTCAGTTGACTTAAC AATTTTTTCTCTTCATATTGCGGGAATATCTTCTATTCTAGGAGCTATCA ATTTTATTTCAACAATTTTAAATATACGACATAAAAATTTTTCTTCTGAT AAAATTCCTTTACTTGTTTGATCTATTTTAATTACAGCAATTTTACTTCT TCTTTCTTTACCAGTTCTTGCTGGAGCTATTACTATACTTTTAACTGACC GAAATCTTAATACTTCCTTTTTTGATCCCTCAGGCGGAGGAGACCCTGTT CTTTATCAACATTTATTTTGATTTTTTGGTCACC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-02-03-at-1.52.46 PM.png,",31.9708128,-81.15865149999999,01/20/2025,"",,,Whole arthropod,Standard electrophoresis system,0.25 TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-02-at-2.19.33 PM.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,GGgatgGTAGGAACTAATTTGAGAATTTTAATTCGAACAGAGTTAGGTCA GCCTGGATCTTTAATTGGAGATGATCAGATTTATAATGTTATTGTTACTG CTCACGCTTTTATTATAATTTTTTTTATGGTAATGCCAATTATAATTGGA GGGTTTGGTAATTGATTAGTTCCTTTAATATTGAGAGCTCCAGATATAGC TTTCCCACGAATGAATAATATAAGATTTTGGTTATTACCTCCATCTCTGA TTTTATTATTATCAGGGGGCCTAGTAGATAGAGGGGCTGGGACAGGTTGG ACAGTGTATCCTCCTTTATCATCAGTGATTTCTCATGGAGGGGCTTCAGT GGATATAACTATTTTTTCTTTACATTTGGCAGGGGTTTCTTCTATTTTGG GAGCCATTAATTTTATTACTACTGTGATTAATATACGTCCAGAAGGTTTA AAGCTTGATCGTGTTCCTTTATTTGTTTGATCAGTAGCTATTACTGCTTT ATTATTATTGCTTTCTTTACCGGTTTTAGCAGGAGCTATTACGATATTAT TAACTGATCGAAATTTAAATACATCTTTTTTTGATCCAGCCGGAGGCGGA GATCCTATTTTATATCAACATTTATTTTGATTTTTTGGT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5283.jpeg,",41.7756400000,-88.0970000000,09/03/2024,"
Collected by Fall 2024 students.<\/p>\n",Formica pallidefulva,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5325-1.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Ayesha-Waheed_IMG_0008-scaled.jpg,",41.7756400000,-88.0970000000,09/03/2024,"
Collected by Fall 2024 students<\/p>\n",Formica pallidefulva,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-12-at-10.24.21 PM.png,","",No,ATAATATGTTGATATAGATTGGGTCCCCCCTCCTGAAGGGTCAAAAAATGAAGTATTTAAGTTTCGGTCAGTTAATAATATTGTAATAGCCCCAGCTAAAACGGGAAGGGATAATAAAAGTAAAATTGCAGTGATTAAGATAGATCATACAAGTAGGGGAATTTTATCAATAGAAAAATTTTTATGATGTATATTAAGAATTGTTGAAATAAAATTAATAGCCCCTAAAATAGATGATATACCGGCAATATGAAGAGAAAAAATTGTTAAGTCTACAGAAGGTCCATTATGAAAAATATTTGAAGCTAAAGGAGGGTAGATAGTTCATCCAGTTCCAGTACCATCATTAATAAAGTTTCTTAAAAGTAATAGAGTGATGGAAGGAGGTAAAAGTCAAAATCTTATATTATTTATACGAGGATAAGCTATATCTGGTGACCCTAATATTAAAGGAATTAGAAAATTTCCAAATCCACCAATTATAAATGGTATTACTATAAAAAAGATTATAATAAAGGCATGATTAGTTACTAAAGAATTATAAATTTGATCATTATTAATTAATGAATTTGAAGAACCTAATTCTAATCGAATAATTATACTTATAGAAGATCCAATTATTCCAGCTCAGATAGCAAATAAAAAATAAAGAATTCCAATATCTTTATGATTTTGTTGACCCAATAATATGTTGATATAGATTGGGTCCCCCCTCCTGAAGGGTCAAAAAATGAAGTATTTAAGTTTCGGTCAGTTAATAATATTGTAATAGCCCCAGCTAAAACGGGAAGGGATAATAAAAGTAAAATTGCAGTGATTAAGATAGATCATACAAGTAGGGGAATTTTATCAATAGAAAAATTTTTATGATGTATATTAAGAATTGTTGAAATAAAATTAATAGCCCCTAAAATAGATGATATACCGGCAATATGAAGAGAAAAAATTGTTAAGTCTACAGAAGGTCCATTATGAAAAATATTTGAAGCTAAAGGAGGGT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-12-at-9.49.47 PM.png,",41.77564,-88.0970,01/23/2025,"
Collected by Spring 2025 faculty<\/p>\n",Polistes dominula,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-12-at-9.54.28 PM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_2623-scaled.jpg,",40.8009423000,-77.8614940000,03/25/2025,"
It was a sunny day. The bug was found dead on a sidewalk. Springtime was here, and one of the wings was gone.<\/p>\n",Coccinellidae,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"","
I spilled some of the buffer when I was extracting the DNA.<\/p>\n",Unknown,N/A,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0153.jpg,",41.7784418,-88.0952683,09/03/2024,"
Collected by fall 2024 students.<\/p>\n",Oncopeltus fasciatus,DNeasy (Qiagen) blood and tissue kit.,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0426-scaled.jpg,","",No,>2-B_AB3_Co1_R (1) edited Graph.ab1.scf (622 bp) TCCTGTAGGGTCAAAGAATGAGGTATTAAAGTTTCGATCAGTTAATAGTATTGTGATTGCTCCTGCTAATACAGGTAAGG ATAATAGAAGCAATAGAGCTGTAATTCCTACTGATCATACAAATAAAGGGGTTTTTTCTAAAGACATACCTGTTGGTCGT ATGTTAATAATTGTTGAAATAAAATTAATTGCACCCAGAATTGATGAAATTCCTGCTAAATGTAGAGAAAAAATAGCCAT ATCTACAGATGCTCCTCTGTGAAAAATTCTATTAGATAGAGGAGGATAAACAGTTCATCCTGTTCCAGCTCCTCTTTCTG TAATTCTTCTAGATAAAAGAAGGGTTAATGAAGGTGGTAATAATCAGAATCTTATATTATTTATTCGTGGAAATGCTATA TCAGGTGCTCCAATTATTAAAGGTACTAGTCAGTTTCCGAAGCCACCAATTATAATTGGTATTACTATAAAGAAAATTAT AATAAATGCATGGGCTGTTACAATTACATTATAAATTTGGTCATTTCCAATAAATATACCTGGTTGACCTAATTCAATTC GAATAATTCATCTTATTGATGAACCTACTATTCCAGATCATATTCCAAATATAAAGTATAGG,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/2-B_AB3_Co1_R-1-edited-Graph-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_3113.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_3114.jpg,",42.4669198000,-73.2014111000,02/12/2025,"
This Blaptica Dubia roach was purchased from a local Petco store, where it was part of a captive colony.<\/p>\n
<\/p>\n",Blaptica dubia,Monarch DNA extraction (NEB),Partial abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_3517-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_2747-1.jpg,","
For the DNA extraction we used the insect adaptation of the protocol<\/p>\n
On March 4th 2025 we did a single PCR with Arthropod primers which used:<\/p>\n
Taq polymerase used: New England Bio Labs One Taq<\/p>\n
Annealing temperatures for PCR reaction: 49 C<\/p>\n
On March 25th 2025 we did a duplex PCR with Arthropod and Wolbachia primers which used:<\/p>\n
Taq polymerase: NEB OneTaq Hot Star Quick-Load 2xMM w\/ Stand. Buffer (M0488S)<\/p>\n
Annealing temperatures for PCR reaction: 49 C<\/p>\n
On April 11th we are running the gel of the results of March 25th using:<\/p>\n
Taq Polymerase: New England Biolabs OneTaq Hot Start Quick-Load 2x MM w\/ Standard Buffer (product #M0488S)<\/p>\n
DNA Ladder used: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product #N0559S)<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5174-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3858-scaled.jpg,",40.7933949000,-77.8600012000,03/31/2025,"
I found it on the sidewalk at around 2:30 PM. It had just rained and was very sunny and warm.<\/p>\n",Araneae,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5277-1-scaled.jpg,","
My arthropod was in lane 2. The rest are controls except for lane 3 (my partner’s results).<\/p>\n",No,TAGTAGGAACAGCGATAAGAGTATTAATTCGTATAGAATTAGGTCAAACCGGAAATTTTATGGGTGACGATCATTTGTAT AATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATACCTATTATAATTGGGGGTTTTGGTAATTG ATTGGTTCCTTTAATGTTAGGGGCGCCGGATATAGCTTTTCCACGAATAAATAATTTGAGTTTTTGATTATTACCCCCCT CTTTAATATTATTGTTTATCTCTTCTATAGCGGAAATAGGAGTTGGAGCTGGATGAACGGTGTATCCGCCTTTAGCATCT ATTGTAGGTCATAATGGAAGTTCGGTGGATTTTGCTATTTTTTCCTTGCATTTGGCTGGTGCTTCGTCTATTATAGGGGC TATTAATTTCATTTCTACTGTTATTAATATGCGATCGGTTGGAATATCTTTAGATAGGGTACCTTTGTTTGTATGATCTG TAGTTATTACAGCCGTCTTATTACTTCTTTCATTACCAGTACTGGCTGGTGCTATTACTATATTGTTGACGGACCGTAAT TTTAATACTTCTTTTTTTGACCCTGCAGGAGGGGGGG,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/HAV1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_5919-scaled.jpeg,",33.9888458186,-84.4397408600,11/12/2024,"",Euryurus,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_9002-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_9003-1.jpeg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_1480-scaled.jpg,",33.9886891046,-84.4397259307,11/12/2024,"
fine legs, long smooth body, mistook for an earthworm at first, patterned on side of body<\/p>\n",Diplopoda,DNeasy (Qiagen),Gonads dissected,MiniPCR,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/6w.png,","
we pipetted the samples into the wells, added buffer, and let the gel run. we got results, but they are not very good.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_1491-scaled.jpg,",33.9892669000,-84.4404690000,11/12/2024,"
spider, yellow and black on legs, taken off of its web, small maybe few millimeters, very active while in captivity<\/p>\n",Mangora placida,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/6w-1.png,","
gel electrophoresis was run, we did not get good results on any of them.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-17-154701-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-17-154634-1.png,",33.9884094663,-84.4386693345,11/12/2024,"
Medium brown body<\/p>\n
Light brown and black spots decorate the specimen’s back<\/p>\n
Ombre light to medium brown legs<\/p>\n
2 antennae coming out of the head with 5 segments each<\/p>\n
Many segments making up its body<\/p>\n",Julidae,DNeasy (Qiagen),Gonads dissected,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/8-wol-4.jpeg,","
Mini gel was ran separately from arthropod so results show only for Wolbachia.<\/p>\n
Lane 2 shows negative for Wolbachia.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0515-rotated.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0516-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_2874-scaled.jpg,",33.9902315491,-84.4384620561,11/12/2024,"
The larvae was burrowed into a piece of dead wood.<\/p>\n",Cerambycidae,DNeasy (Qiagen),Larva,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-27-at-10.10.57 AM.png,","
My sample is in lane 5.<\/p>\n",No,NNNNNNNNNNATTTTTGGAGCNTGAGCAGGNATANTTGGAACTTCTCTAAGACTCCTAATTCGTACAGAACTAGGAAACCCTGGATCTTTAATTGGTGACGATCAAATTTATAATGTTATTGTTACCGCTCATGCATTTATTATAATTTTTTTTATAGTTATACCTATTATAATCGGGGGGTTTGGAAATTGATTAGTTCCCTTAATATTAGGAGCACCAGATATAGCTTTCCCTCGTATAAATAATATAAGATTTTGATTACTTCCTCCCTCATTAACATTATTAATTATAAGAAGAATTGTAGAAAGAGGGGCTGGTACAGGATGAACTGTATACCCACCCCTATCTTCTAACATTGCTCACGGAGGTTCTTCTGTTGATTTGGCTATTTTCAGTCTTCATTTAGCAGGTATTTCTTCCATTTTAGGGGCAGTTAACTTTATCACTACAGTTATTAATATACGACCAGTTGGAATAAGACCAGATCGAATACCTCTCTTTGTTTGAGCTGTTGTAATTACTGCTGTTCTTTTATTACTCTCTTTACCTGTCTTAGCAGGGGCAATTACTATACTTTTAACAGATCGTAATCTTAATACTTCATTTTTTGATCCAGCTGGTGGGGGAGACCCAATTTTATACCAACATCTATTTTGATTTTTTGGTCNCNNN,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-17-155730.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-17-155701.png,",33.9881925612,-84.4390377150,11/12/2024,"
Segmented grey\/brownish body<\/p>\n
Orange separating each segment<\/p>\n
Orange spots on each side and middle of each segment<\/p>\n
Cream colored legs<\/p>\n",Euryurus leachii,DNeasy (Qiagen),Gonads dissected,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/8-wol-3.jpeg,","
Lane 3 shows the positive results for the Wolbachia Gel Electrophoresis.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0517-rotated.jpeg,",33.9957883000,-84.4697663000,11/12/2024,"
This ant was found crawling on a tree. Under the tree there appeared to be a nest covered in leaves where other ants walked out of.<\/p>\n",Formicidae,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-27-at-10.10.57 AM.png,","
Lane 11 contains my sample for this arthropod.<\/p>\n",No,NNNNTNNNNNNNGNCNTCTGANCTGGATAATTGGATCTTCNATAAGAATACTTATTCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTAATTCTATATTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCCTTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATCAGTTTACTTTTATTAAGAAATTTCATTAATGACGGAGTGGGAACAGGATGAACTGTTTACCCTCCATTAGCTTCTAATATTTTTCATAATGGTCCTTCAGTTGACCTGACAATTTTTTCTCTACATATTGCAGGAATATCTTCTATTCTAGGAGCTATCAATTTTATTTCAACAATTCTAAATATACGACATAAAAATTTTTCTTCTGATAAAATCCCTTTACTTGTTTGATCTATTTTAATTACAGCAATTTTACTTCTTCTATCTTTACCAGTTCTAGCTGGAGCTATTACTATACTTTTAACTGACCGGAACCTTAATACTTCCTTTTTTGACCCCTCAGGAGGAGGAGACCCTGTTCTTTTATCAACATTTATTCTGATTTTTTGGTCACCCTGAANTTNAAAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/JS-scaled.jpg,",33.9886254726,-84.4400280421,11/12/2024,"
Exhibits a brownish-black appearance, has ten appendages including jaws, is very hairy, has a black, roundish abdomen with white dots and a heart shaped thorax. Also appears to have black dots on appendages.<\/p>\n
<\/p>\n",Salticidae,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/8-wol.jpeg,","
Lane 1 includes the DNA marker of double-stranded DNA fragment sizes 10k, 6k, 3k, 2k, 1k, 800, 600, 400, and 200 bp. The Gel shown is of the Wolbachia protocol, testing for the 16s rRNA gene only using specific primers. Lane 6 is of my jumping spider arthropod sample, with the negative arthropod control in lane 9 and the negative DNA control in lane 10. The positive arthropod control and the positive DNA control were not run in the Wolbachia protocol gel due to errors during preparation. The Arthropod protocol gel is not shown due to unclear results.<\/p>\n",Unknown,GGGGGCTCTCTTGGTTAGCTGGTTTCCTCGTNGCTAGGC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_8663-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_8671-scaled.jpeg,",33.9895144560,-84.4393455095,11/12/2024,"
The fire ant was crawling very quickly and seemed very active. It was hard to contain it in a container since it moved constantly. In terms of physical characteristics, its color was red and black, had a hard exoskeleton, and had 6 legs and antennas.<\/p>\n",Solenopsis invicta,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Capture-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Capture-1-3.png,","
PCR Procedure:<\/p>\n
\n
Remove all unnecessary items from your lab station<\/li>\n
\u00a0Put on nitrile gloves and clean all surfaces by wiping down with 70% Ethanol.<\/li>\n
\u00a0Collect a 1.5 mL microcentrifuge tube . Label the tube \u201cA\u201d<\/strong>\u00a0(for Arthropod PCR Cocktail). Place the tube in a 1.5mL tube rack.<\/li>\n
Collect six 0.2 mL PCR tubes . Number and label them with your initials. Place the tubes in a PCR tube rack and record putative identification of each arthropod in the table.<\/li>\n
\u00a0Use a P-2 or P-20 pipette to\u00a0add 2 \u03bcL of template DNA to each corresponding Arthropod PCR tube above. It is critical that you change tips between each tube.<\/li>\n
\u00a0Set aside the PCR tubes for now. Place the template DNA tubes back into storage. You will need these again for the Wolbachia\u00a0<\/em>PCR<\/li>\n
Use a P-200 pipette to add each of the following reagents\u00a0(from the \u201cTotal for 7 reactions\u201d column) to the 1.5 mL tube marked \u201cA\u201d. Change tips between each reagent, and check off each reagent after it is added.<\/li>\n
Vortex for 5 sec<\/li>\n
Place the A tube on one side of the mini-centrifuge and the B (balancer tube) on the opposite side. This is called \u201cbalancing\u201d the rotor; if the tubes are not balanced, it will make a loud sound and could damage the microcentrifuge.\u00a0Quickly spin down \u00a0(~ 3-5 seconds) the Arthropod PCR Cocktail to collect liquid at the bottom of the tube.<\/li>\n
Use a P-200 pipette to\u00a0add 23 \u00b5L of the Arthropod PCR Cocktail (A) to tubes 1-6 \u00a0(which should already contain the template DNA). Change tips between each tube. Never place a used pipette tip into the PCR Cocktail because it will contaminate all downstream reactions.<\/li>\n
Tightly secure the lids on each tube.<\/li>\n
Change the rotor \u00a0on your mini-centrifuge to the PCR tube rotor. Center tubes 1-3 on one side and 4-6 on the other side to balance the rotor.\u00a0Briefly spin \u00a0to collect all liquid at the bottom of the tubes.<\/li>\n
Transfer the tubes to the thermal cycler. Once everyone has placed their samples in the thermocycler, the program can be started \u00a0with the Arthropod PCR protocol. If using MiniOne, you may observe the PCR program as it cycles.<\/li>\n
Repeat all the steps for Wolbachia PCR<\/li>\n
Clean up your lab station and wipe surfaces with ethanol<\/li>\n
When the thermal cycler is done, store samples in the refrigerator (4\u00b0C).<\/li>\n<\/ol>\n
Gel Electrophoresis Protocol:<\/p>\n
\n
\u00a0Prepare the Gel with the DNA stain<\/li>\n
Once the gel is ready, place the combs into it and let it cool down<\/li>\n
Once the gel is cooled down and the wells are properly made within the gel, begin pipetting the samples<\/li>\n
Pipette 10uL of the DNA ladder in the first well. Hover your pipette above the well, and slowly empty your pipette<\/li>\n
Continue in this manner, carefully pipetting 10uL of each sample\/sample loading buffer mixture into separate wells in the gel. Change tips between each sample<\/li>\n
Place the lid on the gel box, connecting the electrodes appropriately<\/li>\n
Turn on the power supply to about 100 volts. Maximum allowed voltage will vary depending on size of the electrophoresis chamber<\/li>\n
Let the power run until the yellow (or bottom) band in the loading dye is \u00be down the gel. Then, turn off the power, disconnect the electrodes, and remove the lid and the gel using gloves.<\/li>\n
Place the gel on the transilluminator and note the presence\/absence of bands within each well.<\/li>\n<\/ol>\n",No,My gel electrophoresis results did not show any visible bands for either arthropod DNA or Wolbachia DNA, indicating that my samples likely did not contain a successful arthropod sequence. The absence of arthropod DNA suggests that there may have been experimental errors during the process, which could include improper or incomplete DNA extraction, pipetting mistakes, degradation of the DNA samples, or errors during the PCR setup such as incorrect reagent concentrations or thermal cycling conditions. Because neither the arthropod nor Wolbachia DNA was amplified, I cannot confidently determine whether the arthropods I tested were truly free of Wolbachia, and the results are likely due to a procedural error rather than a biological absence.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_6979.HEIC.jpeg,",33.9904904,-84.4395146,11/12/2024,"
The baby termite has a thicker head and had large pinchers on its mouth. The baby termites head is darker having black pinchers and a dark orange head, while the body is a light tan color. The arthropod is small about one centimeter. The baby termite also has to large antennas on its head.<\/p>\n",Coptotermes formosanus,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-03-at-10.37.33 AM.png,","
We did not have good bands resulting in inconclusive results.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_6981.HEIC.jpeg,",,,11/12/2024,"
The arthropod had some orange on the sides and many legs, but otherwise the arthropod was dark in color other than its legs which were pale or tan. The exoskeleton is hard and thick. The arthropod had two antennas on the top of its head. When threatened the arthropod curls up.<\/p>\n",Euryurus evides,DNeasy (Qiagen),Gonads dissected,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-03-at-10.37.33 AM.png,","
We did not have good bands resulting in inconclusive results.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_1491-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_1479-scaled.jpeg,",33.9526020000,-84.5499327000,11/13/2024,"
Tends to hide from the light, crawls fast towards darker areas trying to escape light. Found at Petco for classroom pet food. No location written on website purchased to where the mealworms were cultivated.<\/p>\n",Arthropoda,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6942.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6950-1.jpeg,","
Gel Images: Lane 7 for both<\/p>\n
(checking for Wolbachia presence and Arthropod extraction verification)<\/p>\n",Unknown,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/21_Vidya_mealworm-CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_1501-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_1498-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_1469-scaled.jpeg,",33.9892669000,-84.4404690000,11/12/2024,"
Slow, does not try to climb up cup when captured, small sized (about .5 cm in length from start of head to end of abdomen)<\/p>\n",,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6942-3.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6950-3.jpeg,","
Gel Images: Lane 6 for both<\/p>\n
(checking for Wolbachia presence and Arthropod extraction verification)<\/p>\n",Unknown,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/20_Vidya_ant-CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_2527.jpeg,",41.7759101642,-88.0956466783,09/03/2025,"
Collected by Fall 2024 students.<\/p>\n",Cicadellidae,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_2722-scaled.jpeg,","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/94-B_AG3_Co1_R.fa,, "",40.8015726,-77.860891,03/25/2025,"
Found on Campus. Sunny day, but cold out. Alive<\/p>\n",Formicidae,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5277.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screen-Shot-2025-04-08-at-12.59.53-PM.png,",38.8196500000,-77.1702100000,03/11/2025,"
Long legs, bulging eyes, dark brown color<\/p>\n",Hemiptera,DNeasy (Qiagen) blood and tissue kit,,,TBE,SYBR Safe,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hymenoptera-2-Tyler-Clark-24-February-scaled.jpg,",38.8187374053,-77.1732624990,03/11/2025,"",Prenolepis imparis,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Pd-2-C1-6-Wol.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Pd-2-C1-6.png,","
Lane 1: Ladder<\/p>\n
Lane 2: Sample 1 (C1)<\/p>\n
Lane 3: Sample 2 (C2)<\/p>\n
Lane 4: Sample 3(C3)<\/p>\n
Lane 5: + Drosophila Control<\/p>\n
Lane 6: – Drosophila Control<\/p>\n
Lane 7: Wolbachia DNA Positive Control<\/p>\n
Lane 8: H20 Control<\/p>\n",Yes,>Prenolepis_imparis2C1-CO1F (637) CTGAGCTGGAATAATTGGATCTTCAATAAGAATACTTATTCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTAATTCTATAGTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCCTTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATCAGTTTACTTTTATTAAGAAATTTCATTAATGACGGAGTAGGAACAGGATGAACTGTTTACCCTCCATTAGCTTCTAATATTTTTCATAATGGTCCTTCAGTTGACCTGACAATTTTTTCTCTACATATTGCAGGAATATCTTCTATTCTAGGAGCTATCAATTTTATTTCAACAATTCTAAATATACGACATAAAAATTTTTCTTCTGATAAAATCCCTTTACTTGTTTGATCTATTTTAATTACAGCAATTTTACTTCTTCTATCTTTACCAGTTCTAGCTGGAGCTATTACTATACTTTTAACTGACCGGAACCTTAATACTTCCTTTTTTGATCCCTCAGGAGGAGGAGACCCTGTTCTTTATCAACATTTATTCTGA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Prenolepis_imparis2C1-CO1F-1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hymenoptera-1-Tyler-Clark-24-February-scaled.jpg,",38.8196500000,-77.1702100000,02/24/2025,"",Prenolepis imparis,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-08-11.14.33-AM.png,","
Lane 1 – Ladder<\/p>\n
Lane 2 – Sample 1<\/p>\n
Lane 3 – ENTRY SAMPLE<\/p>\n
Lane 4 – Sample 3<\/p>\n
Lane 5 – Drosophila Positive Control<\/p>\n
Lane 6 – Drosophila Negative Control<\/p>\n
Lane 7 – Wolbachia DNA Positive<\/p>\n
Lane 8 – Water<\/p>\n
<\/p>\n",No,>2C2 AATTGGATCTTCAATAAGAATACTTATTCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTA ATTCTATAGTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTT TTAGTTCCTTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTC AATCAGTTTACTTTTATTAAGAAATTTCATTAATGACGGAGTGGGAACAGGATGAACTGTTTACCCTCCATTAGCTTCTA ATATTTTTCATAATGGTCCTTCAGTTGACCTGACAATTTTTTCTCTACATATTGCAGGAATATCTTCTATTCTAGGAGCT ATCAATTTTATTTCAACAATTCTAAATATACGACATAAAAATTTTTCTTCTGATAAAATCCCTTTACTTGTTTGATCTAT TTTAATTACAGCAATTTTACTTCTTCTATCTTTACCAGTTCTAGCTGGAGCTATTACTATACTTTTAACTGACCGGAACC TTAATACTTCCTTTTTTGACCCCTCAGGAGGAGGAGACCCTGTTCTTTATCAACATTTATTCTGA,,, "",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/96C82518-5831-4D8C-951F-779AB7845831_1_105_c.jpeg,",38.8177136,-77.1685932,03/12/2025,"",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6731-scaled.jpg,",38.8218452000,-77.1542684000,03/12/2025,"",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hymenoptera-Julia-Shi-9-March.png,",38.8887282000,-77.1491670000,03/09/2025,"",Tapinoma sessile,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-23-143904.png,","",No,ATAAGAATAATTATTCGTATTGAATTAGGGACCTGCGGAGCTTTAATTAGTAATGATCAAATCTATAATTCAATTGTAACAGGACATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTAGAAGTGGGGGCTTTGGAAATTTTTTAGTCCCCTTAATACTCGGAGCCCCAGATATACCTTACCCCCGAATAAATAATATAAGATTTTGATTACTACCGCCCTCAATCCTTTTACTAACTATTAGAAATTTTATCAGATCTGGGGTAGGGACCGGATGAACAGTTTACCCGCCCCTAGCATCTAATATCTATCATAATGGACCTTCTGTAGATTTATCTATTTTCTCCCTTCATATTGCTGGTATATCCTCAATTTTAGGGGCAATTAATTTTATTTCTACTATTATTAATATACACCATAAAAATTTTTCCACAGATAAAATCCCTCTTTTAGTATGATCAATCTTAATTACTGCAATTTTATTACTCCTATCTCTCCCAGTTCTAGCTGGGGCTATTACTATACTTTTAACAGATCGAAACTTTAAATACATCCTTTTTTGACCC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hymenoptera-Jaslene-Hernandez-12-March.jpg,",38.8887282000,-77.1491670000,03/09/2025,"",,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Safe,"","",No,>Little_Black_Ant_6C7 TGGNATAATTGGTTCCTCTATAAGAATAATTATTCGATTAGAATTAGGTTCTTCTAATTCATTAATTAATAATGATCAAATTTATAACTCTATAATTACAAGACATGCATTTATTATAATTTTTTTTATAGTTATACCCTTCATAATTGGTGGATTTGGTAATTTTCTTGTGCCCTTAATATTAGGATCACCTGATATAGCCTACCCTCGTATAAATAATATAAGATTTTGACTCTTACCCCCTTCAATTTCTCTTCTTTTATTAAGAAATTTTATTAATGATGGAGTTGGAACAGGATGAACTATTTACCCCCCATTAGCTTCAAATATTTTTCATAATGGCCCCTCAATTGATTTAACTATTTTTTCCCTTCATATTGCCGGAATTTCCTCTATTTTGGGGGCTATTAATTTTATTTCAACCATTATAAATATACATCATAAAAATTTTTCTATTGATAAAATACCNTTACTTGTATGATCAATTTTAATTACTGCANTTTTATTACTTNTATCTCTNCCAGTTCTTGCNGGAGCTATTACAATACTNNTAACTGACCGTAATCTTAATACTTCATTTTTTGANCCATCAGGAGGAGGNGATCCTATTTTATATCAACATCTTTTNTGATTTTTTGGTCACCCTTGAAGT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-07-at-3.46.22 PM.png,",,,03/12/2025,"
Very small arthropod that was found rolled up, and still seems to be almost rolled up. It has a distinct light and dark gray pattern on the outside, and has many legs inside its shell.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hemiptera_Daniel_Choi_11_March-1-1-scaled.jpg,",38.8179677000,-77.1697061000,03/14/2025,"",Gerridae,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,MiniPCR,TBE,SYBR Safe,"","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-07-at-2.49.55 PM.png,",,,03/12/2025,"",Diptera,DNeasy (Qiagen) blood and tissue kit,,MiniPCR,TBE,SYBR Safe,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-02-at-2.58.13 PM.png,",38.8182275550,-77.1688187800,03/12/2025,"",Hemiptera,DNeasy (Qiagen) blood and tissue kit,,MiniPCR,TBE,SYBR Safe,"","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hemiptera-2-March-19-Teju-rotated.jpg,",38.8181260754424,-77.16855608187076,03/19/2025,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-07-145107.png,",38.818468,-77.173213,03/12/2025,"
It was able to walk and skim on water.<\/span><\/p>\n",,DNeasy (Qiagen) blood and tissue kit,posterior to head,MiniPCR,TBE,SYBR Safe,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hemiptera-1-Tejeswini-Animireddy-19-March-rotated.jpg,",38.8181000000,-77.1680000000,03/12/2025,"",Hemiptera,DNeasy (Qiagen) blood and tissue kit,,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-07-at-11.34.46 AM.png,",38.8181384000,-77.1679748000,02/27/2025,"",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_0699.jpeg,","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/result.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_9171-scaled.jpg,",38.8198674632,-77.1741452531,03/11/2025,"
Found under a log near Indian Run Stream off of Randolph rd.<\/p>\n",Armadillidium nasatum,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Gel-Electrophoresis-Results.png,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/3D3-CO1F-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/487220028_487569347657110_1208237068488016840_n.jpg,",38.8046930000,-77.0435257000,03/11/2025,"
eight legs, banded with alternating dark and light brown coloration. Visible eyes and fangs at the front, darker spotted pattern on abdomen<\/p>\n",Araneae,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,lysis,SYBR Safe,"","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/2B3-CO1F-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/4A0218A0-31E2-4B7D-8B12-DD75B9CDE464-1-scaled.jpeg,",25.8728864000,-81.6692532000,,"",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_8449-scaled.jpeg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-04-at-1.37.34 PM.png,",38.8178752272,-77.1694653364,03/12/2025,"",Aquarius remigis,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_4790-rotated.jpeg,",33.9904935413,-84.4383511692,11/12/2024,"
\n
found climbing up a tree<\/li>\n
very erratic behavior<\/li>\n<\/ul>\n",Nylanderia fulva,DNeasy (Qiagen),Whole arthropod,MiniPCR,TAE,GelGreen,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_4796-rotated.jpeg,",33.9885972371,-84.4386851407,11/12/2024,"
\n
found in soft wood<\/li>\n
move fast<\/li>\n
swarm around<\/li>\n<\/ul>\n",Rhinotermitidae,DNeasy (Qiagen),Whole arthropod,MiniPCR,TAE,GelGreen,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/95E93004-02CD-47C1-8E3D-72DE686D15BC-scaled.jpeg,",33.9532531,-84.5499358,11/12/2024,"
Brown, about 4cm long. Found under a rolled over log, curled into a circle when touched & caught.<\/p>\n",Julidae,DNeasy (Qiagen),posterior to head,MiniOne,TBE,GelGreen,"","
No results were found on the gel as the gel showed no clear bands. Because of this, none of our samples were sent in for sequencing. This is why we cannot be sure if the millipede had Wolbachia<\/em>.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_9012.png,",33.9881208384,-84.4390397111,11/12/2024,"
Found by a log on the side of a riverbank. Dark brown\/black body, relatively small, legs are a lighter brown with a darker brown color on the joins, furry, small pincers.<\/p>\n",Parasteatoda tepidariorum,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/5-arth.jpeg,","
There was most likely an error in our conducting of the lab because of our inconclusive results within our gel. This could have been an issue with the pcr or the DNA extraction.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_9013.jpeg,",33.9882686522,-84.4389724246,11/12/2024,"
Found flying by a rotten log. Moved about for a while, but stayed around the log. Landed directly on my hand when I tried to catch it. It was in a group with other insects of the same species. Small, transparent wings, brown body, small brown legs, head is brown.<\/p>\n",Drosophila melanogaster,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/5-arth.jpeg,","
The band\u2019s show that the controls and the experimental are flawed, indicating that there was an error in the conducting of our lab.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/AA140B58-E88B-4018-9C21-37A5673222F4-scaled.jpeg,",33.9904904000,-84.4395146000,11/12/2024,"
The millipede was found in the soil buried under leave litter and it curled into a spiral upon being collected.<\/p>\n",Polydesmus angustus,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/1-wol.jpeg,","
The DNA PCR process was not succesful because the control bands did not correlate.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/22F94DAC-9603-4687-BB89-3D8F38BD64DE-scaled.jpeg,",33.9904904000,-84.4395146000,11/12/2024,"
The ant was found by a tree stump under a pile of leaves and was very small and quick moving.<\/p>\n",Solenopsis invicta,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/1-wol-1.jpeg,","
The PCR process was not succesful because the control bands did not align.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_9429-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_9423-scaled.jpg,",33.9885777704,-84.4382027612,11/12/2024,"
The flat-backed millipede was found under a rock near a creek squirming around rapidly. They have flattened bodies and a segmented body with many, many legs. They also seem to have small antennae. They have a grey earthy color with bits of orange.<\/p>\n",Polydesmida,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0448-1-scaled.jpg,","
There was no visible band for the sample. The sample was not sent for sequencing.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_9439-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_9437-scaled.jpg,",33.9885097085,-84.4389449208,11/12/2024,"
The baby termite was found under a tree bark near the creek. It seems to be extremely small and has a soft body. They seem to lack an exoskeleton and they are white-cream colored. They lack wings and they have antennae.<\/p>\n",Coptotermes formosanus,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0448-scaled.jpg,","
There was no visible band. The sample was not sent for sequencing.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6565-1-scaled.jpg,",38.8181384000,-77.1679748000,03/11/2025,"
Sunny, 65 degrees F. The water\u00a0strider is black and has some white on it with long legs.<\/p>\n",,DNeasy (Qiagen) blood and tissue kit,,MiniPCR,TBE,SYBR Safe,"","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_2703-scaled.jpeg,",19.5267945000,-154.9941840000,01/31/2025,"",,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_349EF9DF9396-1-1.jpeg,","
Collected manually by hand in Aina loa, Hawai’i.<\/p>\n
Top Row C01(Wells 1-8) includes in order; DNA Ladder, Ant 1,Ant 2, Ant 3, Ant 4, Ant 5,Ant 6, Mosquito 1 (M1).<\/p>\n
Second row (Wells 9-18) includes in order; DNA Ladder, Ant 1,Ant 2, Ant 3, Ant 4, Ant 5,Ant 6, Mosquito 1 (M1).<\/p>\n
Third Row C01 Controls(Wells 16-21) includes in order; DNA ladder, DNA+, Diptera+, Diptera-, H20<\/p>\n
Fourth Row 16S\/Wolbachia \u00a0Controls(Wells 25-29)includes in order; DNA ladder, DNA+, Diptera+, Diptera-, H20<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0129-scaled.jpg,",19.9382406000,-155.1686078000,02/02/2024,"",Arthropoda,Edwards Buffer,Whole arthropod,Edvotek Gel Electrophoresis,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0238-scaled.jpg,","
200 voltage, 2% agarose, below is gel electrophoresis map<\/span><\/p>\n\n\n\n\n\n\n
Ladder<\/span><\/td>\n CLO 1<\/span><\/td>\n CLO 2<\/span><\/td>\n CLO 3<\/span><\/td>\n CLO 4<\/span><\/td>\n CLO 5<\/span><\/td>\n +<\/span><\/td>\n –<\/span><\/td>\n<\/tr>\n
Ladder<\/span><\/td>\n (+) DNA Control<\/span><\/td>\n (-) Water<\/span><\/td>\n <\/td>\n <\/td>\n <\/td>\n <\/td>\n <\/td>\n<\/tr>\n
Ladder<\/span><\/td>\n W 1<\/span><\/td>\n W 2<\/span><\/td>\n W 3<\/span><\/td>\n W 4<\/span><\/td>\n W 5<\/span><\/td>\n +<\/span><\/td>\n –<\/span><\/td>\n<\/tr>\n
Ladder<\/span><\/td>\n (+) DNA Control<\/span><\/td>\n (-) Water<\/span><\/td>\n <\/td>\n <\/td>\n <\/td>\n <\/td>\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n",No,GTATTCTAATCCGAGCTGAACTGGGTCATCCTGGTGCTTTAATTGGAGACGACCAAATTTATAATGTAATCGTCACTGCACATGCTTTTGTTATAATTTTTTTTATAGTGATACCTATTATAATCGGTGGGTTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCTCCTGATATAGCTTTCCCACGAATAAATAATATAAGATTTTGACTTCTTCCCCCTGCTTTATCACTTTTATTAGTAAGAAGTATAGTTGAAAACGGAGCTGGGACAGGGTGAACTGTTTACCCACCTCTATCATCAGGAATCGCCCATGGAGGAGCTTCTGTAGATTTAGCTATTTTTTCTTTACATTTAGCCGGAATCTCTTCAATTTTAGGGGCAGTTAATTTTATTACAACTGTAATTAATATACGATCAACAGGAATTACTCTTGATCGTATACCTTTATTTGTTTGGTCAGTCGTAATTACTGCACTTTTATTATTATTATCTCTTCNAGTTTTAGCCGGGGCTATTACTATGTTACTAACTGATCGAAATTTAAACACATCATTTTTTGACCCTGCAGGAGGAGG,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/3_Ant-CO1F-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_4770-2-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_4768-2.jpeg,",42.4185100000,-72.6305900000,02/09/2025,"
Found in the soil in a potted plant. Body has a dark brown shade, It measures around 8 Mm and has 12 pairs of legs.<\/p>\n",Lamyctes emarginatus,Monarch DNA extraction (NEB),Whole arthropod,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4839-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3701-2-1-scaled.jpg,","
The Taq Polymerase used was the New England Biolabs OneTaq Hot Start Quick-Load 2x MM w\/ Standard Buffer (Product # M0488S). The DNA Ladder used was the New England Biolabs 1 kb DNA Ladder for Safe Stains(Product # N0559S). The date the\u00a0 PCR for the Wolbachia Primer was set up was 3\/27\/2025 at 55\u00b0C. The Date for the Arthropod Primer was set up on 3\/6\/2025 at 49\u00b0C.<\/p>\n
Gel Result Key:<\/strong><\/p>\n
1)DNA Ladder<\/p>\n
2)Lamyctes emarginatus<\/p>\n
3)Genus harmonia<\/p>\n
4)Positive Arthropod Control<\/p>\n
5)Negative Arthropod Control<\/p>\n
6)Positive DNA Control<\/p>\n
7)Water Control<\/p>\n
8)DNA Ladder<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_3644-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3643-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3646-scaled.jpeg,",42.4185200000,-72.6305900000,02/09/2025,"
Ladybird beetle found crawing on a windowsill inside a house.<\/p>\n",Arthropoda,Monarch DNA extraction (NEB),body without wings,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3763.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3758.jpeg,","
JE5 = Lamyctes emarginatus \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 JE3 = Genus Harmonia<\/p>\n
Taq polymerase used: New England Biolavs OneTaq Hot Start Quick-Load 2xx MM w\/ Standard Buffer (Product #M0488S)<\/p>\n
DNA Ladder used: New England Biolabs 1kb Plus DNA Ladder for Safe Stains (product #N0559S)<\/p>\n
PCR 1- Date: 3\/6\/2025 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 Primer: Arthopod \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 Annealing Temperature: 49C<\/p>\n
PCR 2- Date : 4\/3\/2025. \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0Primers – Wolbachia. \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 Annealing Temperature: 55C<\/p>\n
<\/p>\n",No,NNNTTNNTTTTGNNNTATGGGCAGGAATAGTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAGAGGA AGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATACC TATTATAATTGGGGGTTTTGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGATTAAATA ACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGA TGAACTGTTTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTT AGCAGGAATTTCCTCAATTTTAGGTGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTG ATAAAACTCCTTTATTTGTATGATCTGTTCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCA ATTACTATACTATTAACTGACCGAAACTTAAATTCTTCTTTTTTTGACNNAACNNGTGGGGGAGACCCAATTTTATACCA ACATTTATTTTGATTTTTTGNTNNNNNNNNANNTTAAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_6387-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_6388-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_6389-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_6391-scaled.jpeg,",37.6934520000,-97.3382202000,02/19/2025,"
Captive Dubia Roach sourced from dubiaroaches.com (Wichita, Kansas). Kept in enclosure and fed dry dog food and vegetables.<\/p>\n",Blaptica dubia,Monarch DNA extraction (NEB),Rear half,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6922.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6764.jpeg,","
Taq polymerase used: New England Biolabs OneTaq HotStart Quick-Load 2x MM w\/ Standard Buffer(product #M0488S)<\/p>\n
DNA Ladder used: New England. Biolabs 1 kb Plus DNA Ladder for Safe Stains (product #N0559S)<\/p>\n
PCR cocktail was set up on 3\/27\/25 using both Arthropod and Wolbachia primers at an annealing temperature of 49* Celsius.<\/p>\n
Lanes:<\/p>\n
1. DNA Ladder<\/p>\n
2. ELB1-B. dubia<\/em><\/p>\n
3. JBD1- B. dubia<\/em><\/p>\n
4. AC+ Wolbachia positive fruit fly<\/p>\n
5. AC – Wolbachia negative fruit fly<\/p>\n
6. DNA Control<\/p>\n
7. Water<\/p>\n
Also included is a second gel set up for a single reaction with only Arthropod Primers on 3\/13\/25. Lanes are set up the same as the duplex reaction. ELB1 and JBD1 both had significant smearing due to too much DNA.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_9925-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_9926-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_9930-scaled.jpeg,",42.4500845000,-73.2453824000,02/18/2025,"
2 segments- cephalothorax and abdomen, 8 legs, light brown, top of legs are white<\/p>\n",Ozyptila,Monarch DNA extraction (NEB),Whole arthropod,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"","
DNA Extraction Protocol\u00a0<\/strong><\/p>\n
Cell Lysis\u00a0<\/strong><\/p>\n
\n
grind sample for 1 minute with sterile microtube pestle<\/li>\n
add 10 \u03bcl Proteinase K<\/li>\n
Add 200 \u03bcl Tissue Lysis Buffer and immediately mix by vortexing for 10 seconds<\/li>\n
Incubate for 45 minutes @ 56\u00b0C, vortexing samples every 5-15 minutes<\/li>\n
Centrifuge the tube 3 minutes at maximum speed to pellet debris<\/li>\n
Carefully transfer the supernatant to a new labeled 1.5 ml microcentrifuge tube. Discard the tube of cellular debris<\/li>\n<\/ul>\n
DNA Binding and Purification <\/strong><\/p>\n
\n
add 400\u00a0\u03bcl of gDNA Binding Buffer to the sample and mix thoroughly by vortexing for 5-10 seconds<\/li>\n
Label a spin column fitted with a 2.0 ml collection tube<\/li>\n
Pipette the Binding Buffer\/ sample liquid into the spin column without touching the column<\/li>\n
Centrifuge for 3 minutes at 1,000 x g. Keep the tubes in the centrifuge<\/li>\n
Centrifuge for one minute at maximum speed. Transfer the new column to a new column, and transfer spin column to a new collection tube<\/li>\n
Reinsert the spin columns into the collection tubes. Add 500 \u03bcl of Wash Buffer and close the cap<\/li>\n
Centrifuge for 1 minute at maximum, and this time discard both the collection tube and flow through liquid<\/li>\n<\/ul>\n
DNA Elution\u00a0<\/strong><\/p>\n
\n
Transfer the spin column to a new 1.5 ml centrifuge column tube<\/li>\n
Pippette 200 \u03bcl of preheated gDNA (60\u00b0 C) Elution Buffer to the spin column and close the cap<\/li>\n
Incubate at room temperature for 1 minute<\/li>\n
Centrifuge for 1 minute at maximum speed to elute the DNA into the 1.5 ml tube<\/li>\n
Discard the spin column and keep the labeled 1.5 ml tube (which now contains DNA)<\/li>\n
Store the eluted DNA frozen at -20\u00b0 C until PCR<\/li>\n<\/ul>\n
PCR protocol\u00a0<\/strong><\/p>\n
Add template DNA to PCR tubes<\/p>\n
\n
Use a P-2 pipette to add 2\u03bcl of template DNA to each corresponding Arthropod PCR tube, changing tips between each<\/li>\n<\/ul>\n
Prepare PCR Cocktail<\/p>\n
\n
use a p-50 or p-200 pipette to add each reagent (Arthropod_F primer, Arthropod_R primer, Water, Taq Master Mix 2X) to the 1.5mL tube marked “A”, changing tips between each<\/li>\n
close tube and briefly vortex for 5 seconds<\/li>\n
microcentrifuge for about 3-5 seconds<\/li>\n<\/ul>\n
Set up PCR reaction<\/p>\n
\n
Use a P-50 or P-200 pipette to add 23\u03bcl of the arthropod PCR Cocktail (A) to tubes 1-6, changing tips between each<\/li>\n
tightly secure the lids on each tube<\/li>\n
Change the rotor on the mini-centrifuge to the PCR tube rotor. Balance tubes and briefly spin to collect all the liquid at the bottom<\/li>\n
Transfer tubes to thermal cycler<\/li>\n<\/ul>\n
Storage<\/p>\n
\n
store samples in refrigerator at 4\u00b0C<\/li>\n<\/ul>\n
Gel Electrophoresis\u00a0<\/strong><\/p>\n
\n
measure 0.3 grams of agarose powder and add it to a 250mL flask<\/li>\n
add 30 mL 1X TAE running buffer to the flask (a 1% solution). Swirl to mix<\/li>\n
Place a ball of Kimwipe tissue in the flask opening to prevent too much steam from escaping, then heat the agarose\/ buffer mixture in a microwave until the solution becomes clear. About 25 seconds<\/li>\n
Take the flask out of the microwave and swirl to mix<\/li>\n
Let the solution cool to about 50-55C, swirling the flask occasionally so it cools evenly<\/li>\n
Add 5\u03bcl SYBE Safe DNA stain to the agarose\/buffer solutions. swirl to mix. Be careful when pipetting the DNA stain<\/li>\n
Set up a gel casting tray and seal the ends with black rubber stoppers<\/li>\n
Select a comb that will accommodate all samples. Place the comb in the gel casting tray<\/li>\n
Slowly pour the cooled, melted agarose solution into the casting try. Gently pop any bubbled with a pipette tip<\/li>\n
Let gel cool undisturbed on a solid, flat surface until it is opaque and solid.<\/li>\n
Carefully pull out the combs and remove the rubber stoppers<\/li>\n
Place the gel in the electrophoresis chamber with the wells oriented near the (-) electrode<\/li>\n
Add enough 1X TAE running buffer so there is 2-3mm of buffer over the gel<\/li>\n<\/ul>\n
Load the Gel<\/p>\n
\n
Pipette 10 \u03bcl of the DNA ladder in the first well<\/li>\n
Continue carefully pipetting 10 \u03bcl of each sample from PCR into separate wells in the gel. Change tips between each<\/li>\n
Store remaining PCR products in the refrigerator<\/li>\n<\/ul>\n
Run the gel<\/p>\n
\n
place the lid on the gel box, connecting the electrodes appropriately<\/li>\n
Turn on the power supply and set 125 volts for the voltage and 0.2 for the current<\/li>\n
Let the power run until the bottom band in the loading dye is 3\/4 down the gel. Turn off the power, disconnect the electrodes, and remove the lid and gel<\/li>\n<\/ul>\n
Obtain an image of the gel<\/p>\n
\n
Using gloves, place the gel on the transilluminator imaging equipment<\/li>\n
Carefully take a photo of the gel with the transilluminator light on<\/li>\n
Note the presence or absence of bands in each lane<\/li>\n<\/ul>\n
For image of gel go to\u00a0https:\/\/wolbachiaprojectdb.org\/dbpost\/18468<\/a><\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_3254-scaled.jpeg,",37.6871761000,-97.3300530000,02/20/2025,"
Captive bred by and ordered from dubiaroaches.com<\/p>\n",Blaptica dubia,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3406-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3339-scaled.jpeg,","
Taq polymerase: New England Biolabs OneTaq hot start quick-load 2x MM w\/ standard buffer (product #M0488S)<\/p>\n
DNA ladder: New England Biolabs 1 kb plus DNA ladder safe stains (product N0559S)<\/p>\n
Duplex PCR set up on 3\/27\/25 with both arthropod and wolbachia primers at an annealing temperature of 49 Celsius.<\/p>\n
Lanes
\n1- DNA ladder
\n2- ELB1 sample from specimen 1 dubia roach
\n3- JBD1 sample from specimen 2 dubia roach
\n4- AC+ wolbachia positive fruit fly control
\n5- AC- wolbachia negative fruit fly control
\n6- DNA+ wolbachia positive arthropod DNA Control
\n7- water<\/p>\n
Second gel(w\/out annotation) is a single PCR set up on 3\/20\/25 was used but contained too much DNA, however the control samples showed expected results. Lanes contain the same samples in both gels<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/thumbnail_IMG_9127-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/thumbnail_02059494-2AEF-42D1-9B06-48F0D4D9A6F1.jpg,",42.3534866000,-73.3933122000,02/15/2025,"
Flying through living room in winter.<\/p>\n",Anopheles punctipennis,Monarch DNA extraction (NEB),body without wings,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/DNA-Ladder.png,","
TAQ polymerase: NE Bio Labs ONETAQ Hot Start Quick-Load 2x MM w\/ Standard Buffer (product #M0488S).<\/p>\n
DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product #N0559S)<\/p>\n
Annealing temperature at 49C.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Araneae-Ayesha-Khan-11-March-1-rotated.jpg,",38.8199641750,-77.1744184800,03/11/2025,"
Brownish\/black, very small spider. Found in leaf litter that was very moist and close to the soil.<\/p>\n",Araneae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Isopoda-Bhaavya-J-9-March--scaled.jpg,",38.8759200000,-77.4017800000,03/09/2025,"",Isopoda,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Safe,"","",Yes,CTGGGAGTCTCATTGGGGATGATCAAATCTATAATGTAATTGTCACGGCTCATGCTTTTGTAATGATTTTCTTCATAGTAATACCTATTATAATTGGAGGTTTTGGAAATTGATTAATTCCTTTGATATTAGGGGCTCCTGATATAGCTTTCCCTCGTATAAACAATATAAGATTTTGGCTGCTTCCCCCTTCTTTAACTTTACTCCTTAGCAGAGGATTAATTGAAAGAGGAGTAGGAACGGGATGAACTGTTTACCCTCCTTTGGCATCAAGAATTGCCCATAGTGGAGCTTCTGTAGATTTAGGTATCTTTTCTCTTCATCTTGCTGGGGCTTCATCAATTTTAGGGGCAGTAAACTTTATTACGACTATAATTAATATACGGGCGTCCGGAATTAGTATGGATCGTGTGCCTTTATTTGTTTGGTCTGTTTTAGTAACCGCGGTTCTTCTTCTCTTATCTTTACCTGTTCTTGCTGGAGCTATTACTATACTATTGACTGACCGTAATTTAAATACATCTTTTTTTGATCCGAGGGGAGGGGGGGATCCTATTC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hemiptera_Joy_Park_11_March.jpg,",38.8119365781,-77.1649497437,03/11/2025,"
This bug was found in a clear, shallow stream near a bend with slower moving water.<\/p>\n",Aquarius remigis,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,MiniPCR,TBE,SYBR Safe,"","
Lab3_PCR_March2022-1<\/a><\/p>\n",Yes,TTTTTAGGTATATGATCAGGAATAATTGGAATATCTATAAGATGAATTATTCGAATTGAATTAGGTCAACCCGGATCTTTTATTGGAAATGATCAAATTTATAATGTAATTGTAACAGCCCATGCATTTATTATAATTTTCTTCATAGTAATACCAATCATAATTGGGGGATTCGGAAATTGACTTGTACCTTTAATAATTGGAGCACCAGATATGGCATTTCCGCGAATAAATAATATAAGATTTTGACTTTTACCACCATCTTTAACTTTATTACTTGTTAGTAGTTTAGTTGACGCTGGAGCAGGTACTGGATGAACTGTTTATCCACCATTATCTAGAAATATTGCTCATAACGGAGCATCAGTAGATTTAACAATTTTCTCACTTCATTTAGCCGGTGTCTCATCAATTCTAGGAGCCGTAAATTTTATTTCAACTATCATTAATATACGAACAATAGGAATAACTAGAGAAAAAATTCCTCTTTTTGTTTGATCTGTAGGTATTACGGCATTACTTTTATTATTATCTTTGCCTGTTCTTGCAGGAGCAATCACAATATTATTAACAGACCGAAATTTAAATACATCTTTCTTTGATCCAGCTGGAGGAGGGGATCCAGTTCTTTACCAACATTTATTTTGATTTTTTGGTCACCCTTGAAGTTTAAA CAGCCCATGCATTTATTATAATTTTCTTCATAGTAATACCAATCATAATTGGGGGATTCGGAAATTGACTTGTACCTTTA ATAATTGGAGCACCAGATATGGCATTTCCGCGAATAAATAATATAAGATTTTGACTTTTACCACCATCTTTAACTTTATT ACTTGTTAGTAGTTTAGTTGACGCTGGAGCAGGTACTGGATGAACTGTTTATCCACCATTATCTAGAAATATTGCTCATA ACGGAGCATCAGTAGATTTAACAATTTTCTCACTTCATTTAGCCGGTGTCTCATCAATTCTAGGAGCCGTAAATTTTATT TCAACTATCATTAATATACGAACAATAGGAATAACTAGAGAAAAAATTCCTCTTTTTGTTTGATCTGTAGGTATTACGGC ATTACTTTTATTATTATCTTTGCCTGTTCTTGCAGGAGCAATCACAATATTATTAACAGACCGAAATTTAAATACATCTT TCTTTGATCCAGCTGGAGGA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hemiptera-Hrishi-Desai-12-March.jpg,",38.8185870000,-77.1732540000,03/12/2025,"
Collection location was a stream surrounded by several trees and rocks. Temperature was 44 F. Long, tapered, black body, long antennae + legs, small hairs covering body.<\/p>\n",Gerris marginatus,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-02-at-3.37.03 PM.png,",38.8175832828,-77.1685809047,03/12/2025,"
Brown, thin oval bod, 4 long legs with 90 degree bends in the middle, two curled legs in the front<\/p>\n",Hemiptera,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_6732-2-scaled.jpeg,",38.8177183000,-77.1685936000,03/12/2025,"",Aquarius remigis,DNeasy (Qiagen) blood and tissue kit,,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hymenoptera-Patricia-Chang-12-March.png,",38.8206554232,-77.1743781836,03/12/2025,"
Dark brown coloration and has wings<\/p>\n",Prenolepis imparis,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Safe,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hemiptera-Joseph-Chang-12-March-1.png,",38.81372982534192,-77.16744834329018,03/12/2025,"
Water Strider found in a slow-flowing stream.<\/p>\n",Hemiptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hemiptera-1-Tejeswini-Animireddy-19-March-1-rotated.jpg,",38.8201040000,282.8258860000,03/12/2025,"
It has long legs, its medium sized, and it has a dark color<\/span><\/p>\n",Aquarius nyctalis,DNeasy (Qiagen) blood and tissue kit,Gonads dissected,MiniPCR,TBE,SYBR Safe,"","",Yes,>7B5 GTAATATGATCAGGAATAATTGGAATATCTATAAGATGAATTATTCGAATTGAATTAGGTCAACCCGGATCTTTTATTGGAAATGATCAAATTTATAATGTAATTGTAACAGCCCATGCATTTATTATAATTTTCTTCATAGTAATACCAATCATAATTGGGGGATTCGGAAATTGACTTGTACCTTTAATAATTGGAGCACCAGATATGGCATTTCCGCGAATAAATAATATAAGATTTTGACTTTTACCACCATCTTTAACTTTATTACTTGTTAGTAGTTTAGTTGACGCTGGAGCAGGTACTGGATGAACTGTTTATCCACCATTATCTAGAAATATTGCTCATAACGGAGCATCAGTAGATTTAACAATTTTCTCACTTCATTTAGCCGGTGTCTCATCAATTCTAGGAGCCGTAAATTTTATTTCAACTATCATTAATATACGAACAATAGGAATAACTAGAGAAAAAATTCCTCTTTTTGTTTGATCTGTAGGTATTACGGCATTACTTTTATTATTATCTTTGCCTGTTCTTGCAGGAGCAATCACAATATTATTAACAGACCGAAATTTAAATACATCTTTCTTTGATCCAGCTGGAGGAGGGGATCCAGTTCTTTACCAACATTTATTTTGATTTTTTGGTCACCC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Red_Ant.png,",38.8181384000,-77.1679748000,03/12/2025,"
Found in the stream, residing in the water. Located in the shallow part of the water. <\/span><\/p>\n
It was likely dead at the time of collection, so it is possible that it fell into the water.<\/span><\/p>\n
A medium sized ant, light brown, slightly curled up with many legs.<\/span><\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/CO1-Image.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Wolbachia-Image.png,","",No,TAATTGGATCTTCAATAAGAATACTTATTCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTAATTCTATAGTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCCTTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATCAGTTTACTTTTATTAAGAAATTTCATTAATGACGGAGTGGGAACAGGATGAACTGTTTACCCTCCATTAGCTTCTAATATTTTTCATAATGGTCCTTCAGTTGACCTGACAATTTTTTCTCTACATATTGCAGGAATATCTTCTATTCTAGGAGCTATCAATTTTATTTCAACAATTCTAAATATACGACATAAAAATTTTTCTTCTGATAAAATCCCTTTACTTGTTTGATCTATTTTAATTACAGCAATTTTACTTCTTCTATCTTTACCAGTTCTAGCTGGAGCTATTACTATACTTTTAACTGACCGGAACCTTAATACTTCCTTTTTTGATCCCTCAGGAGGAGGAGACCCTGTTCTTTATCAA",,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hymenoptera-Dissection-Wolbachia-Project.png,",38.8181384000,-77.1679748000,03/10/2025,"
It was discovered at a very dry location, in the shade, more ants found by pulling up bark. Made the ant crawl onto leaf, alive during collection date.<\/p>\n",Camponotus,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-23-1.45.18-PM.png,","",No,>7D4 ATAAGAATAATTATTCGTATTGAATTAGGGACCTGCGGAGCTTTAATTAATAATGATCAAATCTATAATTCAATTGTAAC AGGACATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGCTTTGGAAATTTTTTAGTCCCCTTAA TACTCGGAGCCCCAGATATAGCTTACCCCCGAATAAATAATATAAGATTTTGATTACTACCGCCCTCAATCCTTTTACTA ACTATTAGAAATTTTATCAGATCTGGGGTAGGGACCGGATGAACAGTTTACCCGCCCCTAGCATCTAATATCTATCATAA TGGACCTTCTGTAGATTTAGCTATTTTCTCCCTTCATATTGCTGGTATATCCTCAATTTTAGGGGCAATTAATTTTATTT CTACTATTATTAATATACACCATAAAAATTTTTCCACAGATAAAATCCCTCTTTTAGTATGATCAATCTTAATTACTGCA ATTTTATTACTCCTATCTCTCCCAGTTCTAGCTGGGGCTATTACTATACTTTTAACAGATCGAAACTTAAATACATCCTT TTTTGACCCTTCCGGAGGTGGGGACCCAATTTTATATCAACATTTATTTTGATTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/ant-photo.png,",38.8887282000,-77.1491672000,03/09/2025,"",Monomorium,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-23-1.59.14-PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-23-1.58.48-PM.png,","
Sample A3 in the gel images<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hymenoptera-Julia-Shi-09-March.png,",38.9608630000,-77.2077738000,03/09/2025,"",Camponotus pennsylvanicus,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-26-at-10.27.42-AM.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hymenoptera-Jacqueline-Ake-09-March.png,",38.9608630000,-77.2077738000,03/09/2025,"",Camponotus castaneus,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/blk7AW-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/blk7ACO1-scaled.jpg,","",Yes,ATTGGTTCTTCTATAAGAATAATTATTCGATTAGAATTAGGATCCCCTGATTCACTAATTCTTAATGATCAAACCTTCAA TACTATCGTTACAAGCCATGCTTTTATTATAATCTTTTTCATAGTTATACCTTTTATAATTGGAGGATTTGGTAACTTCT TAGTACCCTTAATACTTGGGTCTCCTGATATAGCCTATCCACGCATAAATAACATAAGATTCTGATTACTACCCCCTTCA ATTTCTCTATTAATCTTAAGAAATTTTATTAATGAAGGATCAGGAACAGGTTGAACTATTTATCCCCCCCTATCATCTAA TACCTTTCATAGTGGTCCCTCTATTGATTTAACTATTTTCTCCCTTCATATTGCCGGAATATCATCAATTCTAGGGGCAA TCAATTTTATTTCAACAATTTTAAACATACATAATTCTAATATTACCTTAGATAAAATTCCCCTATTAGTATGATCTATT CTTATCACTGCTATTCTCCTCCTCCTCTCCCTTCCTATTCTTGCAGGAGCTATTACAATACTACTTACAGATCGAAATCT CAATACTTCATTTTTTGANCCCTCANGANGNNGANACCCTATTCTATATCAACNCTTNNTTTGNNTTTTTG,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/7A2-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Untitled-drawing.jpg,",38.8197184734,-77.1702000000,03/12/2025,"
Long, thin body, small compound eyes at the end of the head, six legs, two distinctly larger and two smaller and folded up near the head, dark brown\/black color, three-segmented body<\/p>\n",Aquarius remigis,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3301-scaled.jpg,",38.8174321526,-77.1685453679,03/12/2025,"",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Faxonius-Virillis-Zheng-12-March-scaled.jpeg,",38.8181384000,-77.1679748000,03/12/2025,"
Big, dark, two claw, 8 legs(4 on each side)<\/span><\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,MiniPCR,TBE,SYBR Safe,"","",,,,, "",38.8195673835,-77.1738772731,03/08/2025,"",Gerridae,DNeasy (Qiagen),Abdomen,,,,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-02-2.52.03-PM.png,",38.8182275558,-77.1688187885,03/12/2025,"",Hemiptera,DNeasy (Qiagen) blood and tissue kit,,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-02-at-2.49.31 PM.png,",38.8176062469,-77.1684798102,03/12/2025,"
Collected on the rocky surface of the Indian Run stream.<\/p>\n",Hemiptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-23-at-11.30.27 AM.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-02-at-2.47.35 PM.png,",38.816086586480516,-77.17036372748775,03/12/2025,"
Collected in the water, brown in color, long body. Collected late winter, 52 degrees C.<\/p>\n",Gerridae,DNeasy (Qiagen) blood and tissue kit,Body without legs,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hemiptera-Sina-Farchtchi-12-March-scaled.jpg,",38.8188527643,-77.1735590796,03/12/2025,"",Aquarius najas,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","
Collected on the Indian Run Stream bend next to large boulders.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-02-at-2.46.54 PM.png,",38.8188441135,-77.1736500061,03/12/2025,"
It was collected on the Indian Run Stream bend, floating on the water. Late winter, 52 degrees Fahrenheit, and my arthropod is big and black and has one long body with four legs in the back and two legs coming out in the front.<\/p>\n",Aquarius najas,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/water_strider.jpeg,",38.8196470000,-77.174872,03/12/2025,"
4 brown legs, long nose and black body<\/p>\n",Hemiptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-02-2.48.05-PM.png,",38.81822502185805,-77.16810527113965,03/12/2025,"",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hemiptera-Zoe-Lowe-12-March-scaled.jpeg,",38.81965,-77.17021,03/12/2025,"
On the surface of the steam, near the edge of the stream (close to the bank). Collected by running a net along the surface of the river.<\/p>\n
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<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n",Hemiptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/PXL_20250314_144836214-scaled.jpg,",38.82095175008342,-77.1742222604373,03/12/2025,"",Gerridae,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-02-at-2.52.22 PM.png,",38.8196000000,-77.1702100000,03/12/2025,"
The thief ant was caught near a stream, with lots of rocks, grass, wet soil, twigs, and sticks. It was late winter, at around 55 F, and it was found on the ground. It is small, brown, and has wings.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Coleoptera-Ananya-Bhatt-12-March-scaled.jpeg,",38.804693,-77.0435257,03/12/2025,"
Wet, muddy, rocky, near stream, late winter, Beetle with a rough black exterior, long orange legs (with browning at the joints) and long orange antennae. Has six legs and a (relatively) large body compared to its head.<\/span><\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/D389DC7E-1FC9-4FA1-9E34-D1CEFBE8A229-scaled.jpeg,",25.943535510343583,-81.71630859375,,"",Arthropoda,Blood and Tissue kit,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Arthopod-Labeling-.png,","",Unknown,,,, "",38.8210560000,-77.1748720000,03/12/2025,"
Long dark brown and thin body with 6 legs (2 in the front sides, below head and 4 coming out of the sides to the back) and 2 Antennae coming out of the head.<\/span><\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/co1-edited.png,","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-02-at-2.41.26 PM.png,",38.8183470000,-77.1676610000,02/27/2025,"
The carpenter ant was caught on a rock in front of the school that was near the soil. It was late winter, at around 45 F, with clouds and some wind blowing. It\u00a0is red and it has 2 long antennae. It has short hair strands coming off of its body. It has 6 legs.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-23-at-11.22.44 AM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-23-at-11.22.52 AM.png,","",No,TACTTATCCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTAATTCTATAGTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCCTTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATCAGTTTACTTTTATTAAGAAATTTCATTAATGACGGAGTGGGGACAGGATGAACTGTTTACCCTCCATTAGCTTCTAATATTTTTCATAATGGTCCTTCAGTTGACCTAACAATTTTTTCTCTGCATATTGCAGGGATATCCTCTATTCTAGGAGCTATCAATTTTATTTCAACAATTTTAAATATACGACAT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_0675-scaled.jpg,",44.9259166000,-93.1727958000,09/19/2024,"
\n
Banded sugar ant<\/li>\n
Tail is striped brown and black<\/li>\n
Ant crawled around tube and curled when it touched the ethanol<\/li>\n<\/ul>\n",Formicidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/gel.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/RG1_ant-CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/ew.jpg,",38.8181384000,-77.1679748000,03/12/2025,"
Winged — winter behavior<\/p>\n
Found solo near rotting bark<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/gelgelgel-scaled.jpg,","",No,>6D2 GCCTCTGAGCTGGAATAATTGGATCTTCAATAAGAATACTTATTCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTAATTCTATAGTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCCTTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATCAGTTTACTTTTATTAAGAAATTTCATTAATGACGGAGTGGGAACAGGATGAACTGTTTACCCTCCATTAGCTTCTAATATTTTTCATAATGGTCCTTCAGTTGACCTGACAATTTTTTCTCTACATATTGCAGGAATATCTTCTATTCTAGGAGCTATCAATTTTATTTCAACAATTCTAAATATACGACATAAAAATTTTTCTTCTGATAAAATCCCTTTACTTGTTTGATCTATTTTAATTACAGCAATTTTACTTCTTCTATCTTTACC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/tempImage3Bdx2J-scaled.jpg,",42.4594870000,-73.3146640000,02/19/2025,"
Was still in larvae form not a beetle<\/p>\n",Tenebrio molitor,Monarch DNA extraction (NEB),Partial abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_5397.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5462.jpg,","
\n
March 5th PCR<\/li>\n
taq polymerase use was New England Biolabs<\/li>\n
annealing temperatures for arthropod reaction was 49 celsius, for denaturing was 94 celcuis, and extension 68 celcius<\/li>\n
March 26th PCR<\/li>\n
taq polymerase used New England Biolobs<\/li>\n
Duplex PCR we used both Wolbachia and Arthropod primers<\/li>\n
DNA ladder used New England Biolabs<\/li>\n
annealing temperatures for arthropod reaction was 49 celsius<\/li>\n<\/ul>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_0373-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_0372-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_0371-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_0370.jpeg,",42.4600883480,-73.3156753514,02/19/2025,"",Blaptica dubia,Monarch DNA extraction (NEB),Rear half,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_0461-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/thumbnail_IMG_5461-1.jpg,","
For DNA extraction we did cell lysis, DNA binding and purification, and DNA Elution. Taq polymerase used: New England Biolabs OneTaq Hot Start Quick-Load 2x MM w\/ Standard Buffer. First PCR set up on 3\/5\/25 and was a single PCR with only Arthropod primers and the annealing temperature was 49 C. The second PCR Reaction set up date 3\/26\/25 and was a duplex PCR with both Arthropod and Wolbachia primers and the annealing temperature was 49 C. DNA Ladder used: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/1000005005-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/1000005004-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/1000005002-scaled.jpg,",42.4566993000,-73.3168792000,02/17/2025,"
Laby beetle has very slow movement and fading colors with roughly 4 spots on each side of its back. It has two large spots at its head.<\/p>\n",Harmonia axyridis,Monarch DNA extraction (NEB),Partial abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/1000005196.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/1000005408-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/1000005407-1-scaled.jpg,","
Single PCR Reaction – CO1 Primers 3\/12<\/p>\n
Taq polymerase used: New England Biolabs OneTaq Hot Start Quick-Load 2x MM w\/Standard Buffer<\/p>\n
DNA Ladder:New England Biolabs 1kb Plus DNA Ladder for Safe Stains<\/p>\n
Performed 1 cycle at 94 C, 30 cycles at 94 C, 49 C, and 68 C, and 1 cycles at 68 C. 55C annealing temperature.<\/p>\n
Single PCR Reaction – Wolbachia_F and Wolbachia_R Primers 3\/25<\/p>\n
Taq polymerase used: New England Biolabs OneTaq Hot Start Quick-Load 2x MM w\/Standard Buffer<\/p>\n
DNA Ladder: New England Biolabs 1kb Plus DNA Ladder for Safe Stains<\/p>\n
55C annealing temperature, same cycles as previously stated.<\/p>\n",No,AGTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAGAGGAAGATTAATTGGAAACGACCAAATTTATA ATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTTTGGAAATTGA TTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGC TTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTTTACCCTCCTCTTTCTTCTA ATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCA GTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGATAAAACTCCTTTATTTGTATGATCTGT TCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTATTAACTGACCGAAACT TAAATTCTTCTTTTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Hemiptera-Carla-Galdo-11-March-scaled.jpeg,",38.8199700000,-77.1743000000,03/11/2025,"
Top and legs are brown, and underside is black.<\/p>\n",Hemiptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4678-scaled.jpg,",38.8218452000,-77.1542684000,03/10/2025,"
The arthropod looks yellowish-orange, and has a lot of legs coming out of the sides of its body. Its antennae are thicker than its legs, and at the front it wields a pair of two sharp looking fangs\/pinchers.<\/p>\n",Strigamia,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/C3-Wolbachia-Gel-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/C3-Arthropod-Gel-1.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-02-at-11.12.00 AM.png,",38.8195496909,-77.1737290582,03/11/2025,"
Found mating with other water striders as being collected in shallow stream<\/span><\/p>\n",Hemiptera,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,MiniPCR,TBE,SYBR Safe,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-02-at-11.08.55-AM.png,",38.7240446,-77.10824439999999,03/16/2025,"
Brown; somewhat hairy; larger bristles on legs; furry on the rest of the body. <\/span><\/p>\n",Dysdera crocata,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/3B5-CO1F-2.fa,, "",,,,"",Harmonia axyridis,DNeasy (Qiagen) blood and tissue kit,body without wings,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Gel-DNA-Electrophoresis-Pd3-Group-D.png,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/3D1-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-02-111600.png,",38.9012225000,-77.2652604000,03/01/2025,"
Collected inside home during late winter in urban setting. Approximate temperature 21 degrees Celsius. About 2cm long, dark brown.<\/p>\n",Amaurobius ferox,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Isopoda-Andrew-Raptis-11-March.png,",38.8199966988,-77.1741107235,03/11/2025,"
Caught in the wild during TJHSST stream field trip near Indian Run on March 11 2025<\/p>\n",Isopoda,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Pd-3C-1-6.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/Pd-3C-1-6-Wol.png,","
Chopped roly poly in half and extracted DNA from front half of organism (including entire head and eyes and a fraction of its abdomen)<\/p>\n",No,TGCTTGAGCAGGGGCTGTTGGAACAGCCCTTAGGATAATTATTCGAACTGAGTTAGGAAACCCTGGGAGGTTAATTGGAGACGATCAGATTTATAATGTGATTGTTACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGCTTTGGTAATTGATTATACCTTTAATATTAGGGGCCCCTGATATAGCTTTTCCGCGTATAAATAATATAAGATTTTGGCTTCTTCCCCCATCACTAATTTTGTTATTAAGAAGAGGTTTAGTTGAAAGAGGTGTTGGGACTGGCTGGACTGTCTACCCACCTTTGGCAAGTGGAATTGCCCATAGAGGGGCTTCAGTAGATTTAGGGATTTTTTCCCTTCATTTGGCTGGAGCTTCTTCAATTTTAGGAGCTGTAAATTTTATTACTACTGTAATTAATATACGAGCAGAGGGGATAAGGATAGATCGTGTCCCTCTATTTGTTTGGTCAGTGTTAATTACAGCAATTCTCTTGTTACTTTCACTACCAGTTTTAGCAGGGGCCATTACTATGTTATTAACAGACCGTAATCTGAACACCTCTTTTTTCGACCCTAGGGGGGGAGGAGATCCTATCTTGTACCAACACTTATTTTGATTTTTTGGTCACCCTTGAAGTTTAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-02-at-11.15.08 AM.png,",38.8183470000,-77.1676610000,,"
Collected on a rock in the soil on a cloudy day, 45 deg F. Has short hair strands coming off of its body with 6 legs and 2 long antennae.<\/p>\n",Camponotus,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","",,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/3B4-CO1F-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/PXL_20250313_171757093-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/PXL_20250313_172444762-scaled.jpg,",38.8195663329,-77.1741755080,03/11/2025,"
In stream when caught.<\/p>\n",Aquarius remigis,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/gel-results.png,","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/3D2-CO1F-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-02-at-11.10.10 AM.png,",38.8193328061,-77.1739369052,03/11/2025,"
Reddish brown spider with a big abdomen and multiple legs. Found under a rock on the bank of a stream near the bend in the river.\u00a0<\/span><\/p>\n",Mermessus tridentatus,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Safe,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/147fcccf-af26-4065-a977-d397f4531bfc.jpg,",38.8211047000,-77.1752540000,03/11/2025,"
The pill bug was found near the stream’s bend.<\/span><\/p>\n
Although the habitat was near the stream, pillbugs live on the land. It was found near the bed of the stream, underneath some of the rocks, near where it was dirty and the rock left a sort of dent in the groun.d.<\/span><\/p>\n
The pillbug appears to be somewhat rolled up, but not completely stretched out. Also, it looks to be black and brown, and its legs are curled up. <\/span><\/p>\n",Armadillidium vulgare,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-08-131801.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Diptera-Shana-Agoi-March-11.jpg,",38.8169728000,-77.1620864000,02/27/2025,"",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/IMG_0699-1.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-02-at-11.12.34 AM.png,",38.8177105000,-77.1685742000,02/27/2025,"",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/gel-3.jpg,","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/result-2.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_20250313_132302751-scaled.jpg,",38.8196724746,-77.1694491109,03/11/2025,"
Brown top and legs with silver sides and black underside<\/p>\n",Hemiptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4168-scaled.jpeg,",38.8201680000,-77.1741320000,03/11/2025,"
Collected at stream near TJ, under rocks. Arthropod is winged, has 6 legs and thin waist.<\/span><\/p>\n",Prenolepis imparis,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Safe,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Geophilomorpha1.png,",38.8197421706,-77.1742924567,03/11/2025,"
Found on a log, terrestrial habitat, in 50 degrees Fahrenheit temperature<\/p>\n
Long, thin, several legs, brown color, antennaes<\/p>\n",Geophilomorpha,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Horned-Squash-Bug-Anasa-armigera.png,",38.8185143865,-77.1685533557,,"",Coreidae,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Coleoptera-Hasset-Bekele-11-March.jpeg,",38.8197200000,-77.1741800000,03/11/2025,"
6 legs, 2 antennas at the top of its head<\/p>\n",,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Trichoptera-Sarah-Coppenbarger-11-March-scaled.jpeg,",38.8181384000,-77.1679748000,03/11/2025,"
Collected during late winter and was secured underneath a rock next to the stream. The temperature was 53 degrees F in an aquatic habitat. The arthropod was collected using a dip net and kicking rocks by the stream. The arthropod has a head and first three thoraxes are hardened, 2 fleshy, hairy hooks on the posterior, 3 pairs of segmented legs, gill tufts on abdomen. The specific name of the organism is Hydropsyche betteni. It’s common name is a Common Net Spinning Caddisfly. The organism was stored in ethanol after collection in a microtube.<\/span><\/p>\n",Trichoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/WolbachiaProject_A.verbasci.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/WolbachiaProject_A.verbasciUNDERSIDE.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/WolbachiaProject_A.verbasciWING.jpg,",38.7996299000,-77.2618468000,03/09/2025,"
Very small (~3 mm), elytron with brown, black, and white “zig-zag” pattern. Flying (wings present). Beetle.<\/span><\/p>\n
Collected late winter, in forest on ground, in 56\u00b0F weather on a partly cloudy day.<\/p>\n",Anthrenus verbasci,DNeasy (Qiagen) blood and tissue kit,body without wings,MiniPCR,TBE,SYBR Safe,"","
CO1 Band Produced<\/p>\n
WOL Band produced<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/coleoptera.png,",38.8171918902,-77.1686192043,02/27/2025,"
The arthropod is a black beetle, no other coloring. It is small, width at 0.5 inches. Beetle is slightly curved, legs are still intact. There are ridges among the abdomen.<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Pd-2-B1-3.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Pd-2-B1-6-Wol-1.png,","
Removed exoskeleton and separated abdomen<\/p>\n
Used sand to homogenize<\/p>\n
Did not use RNAse<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Geophilomorpha-Krish-Nair-11-March-scaled.jpg,",38.8199641750,-77.1744184800,03/11/2025,"
In leaf litter that was very moist and close to the soil. Temperature around 53 degrees fahrenheight. Description of organism is Pink-brownish centipede that is skinny. Has an orange hue towards the front end. Order Geophilomorpha. Scientific name identified as Strigamia bidens also known as the “Long Thin Centipede.”<\/p>\n",Strigamia,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Geophilomorpha-Krish-Nair-11-March-scaled.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/PXL_20250313_150004023.MP-EDIT-1.jpg,",38.8199274317,-77.1741893627,02/27/2025,"
Collected on the ground, under a rock, next to stream. It is a tiny black beetle, it has 6 legs and 2 antennae coming from its head. Its shell is a shiny black and its legs are slightly yellow\/orange.<\/span><\/p>\n",Stenolophus ochropezus,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/PXL_20250407_144959382.MP-EDIT-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/CO1-GEL-RUN.jpg,","",No,>2B2 AGTAGGGACTTCCTTAAGTATACTAATTCGAGCTGAATTAGGAACTCCCGGTGCATTAATTGGTGATGATCAAATTTATA ATGTTATTGTAACTGCACATGCTTTTATCATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGAAATTGA TTGGTACCTTTAATATTAGGAGCTCCTGATATAGCCTTTCCTCGAATAAATAATATAAGTTTTTGATTATTGCCTCCTTC ATTAACTCTTCTCCTAATAAGAAGAATAGTAGAAAGAGGAGCTGGGACTGGATGAACAGTTTACCCACCCCTATCATCTG GTATTGCCCATGGAGGTGCTTCAGTTGACTTAGCTATTTTTAGTCTACATTTAGCAGGAGTATCTTCTATTCTAGGAGCT GTAAATTTTATTACTACAATTATTAATATACGATCAGTAGGAATAACATTTGATCGAATACCATTATTTGTTTGATCTGT AGGAATTACTGCTCTACTTCTACTTTTATCTTTACCTGTATTAGCAGGAGCAATTACTATATTGTTAACAGATCGAAATC TAAATACTTCATTTTTTGACCCAGCGGGAGGGGGAGATCCTATTTTATATCAACATTTATTTTG,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/2B2-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Black-Blow-Fly.jpg,",40.5591424000,-75.4025487000,02/25/2025,"
Caught in Penn State Lehigh Valley bathroom.<\/p>\n",Phormia,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Wolbachia-Gel-1.jpg,","",No,TNNNNNNTTCGGAGCTTGATCTGGAATAATTGGAACTTCATTAAGAATTCTAATTCGAGCTGAACTAGGGCACCCTGGAG CTCTAATTGGAGATGACCAAATTTATAACGTAATTGTAACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCA ATTATAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGGGCTCCTGATATAGCTTTCCCACGAATAAACAA TATAAGTTTTTGACTTTTACCTCCTGCATTAACTCTATTGTTAGTTAGTAGTATAGTAGAAAATGGGGCTGGAACAGGAT GAACTGTTTACCCACCTTTATCATCTAATATTGCTCATGGAGGAGCATCTGTTGATCTAGCTATTTTCTCTCTTCACTTA GCAGGAATTTCTTCAATTTTAGGAGCTGTAAATTTCATTACAACTGTAATTAATATACGATCAACTGGAATTACATTTGA TCGAATACCTTTATTTGTTTGATCTGTAGTTATTACTGCTCTATTACTTTTATTATCTTTACCTGTATTAGCCGGTGCTA TTACTATATTATTAACTGATCGAAATTTAAACACTTCATTCTTTGACCCAGCAGGAGGAGGAGATCCTATTTTATATCAA CACTTATTCTGATTTTTTGGTCACCCNNGAAGTTTAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/20250219_151020-scaled.jpg,",25.8728864000,-81.6692532000,,"
found in the landscape<\/p>\n",Culicidae,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system Bio-Rad,TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Wolbachia-Gel-Electrophoresis.jpg,","
the petri dish in the photo above was mislabeled. this one is supposed to be arthropod 2 or (insect 2)<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Bug.jpg,",42.4600467791,-73.3158180110,02/18/2025,"
The fruit fly is from a captive collection from the Berkshire Community College Campus in Pittsfield, MA. The fruit fly has red eyes, transparent wings.<\/p>\n",Diptera,Monarch DNA extraction (NEB),Whole arthropod,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Wolbachia-Primers.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Arthropod-Primers.png,","
For the DNA extraction we followed the protocol with no issues. The 1.5ml tubes were in the incubator for 37 minuets. We followed the insect adaptation of the protocol.<\/p>\n",Yes,AGTTGGAACATCTTTAAGAATTTTAATTCGAGCTGAATTAGGACATCCTGGAGCATTAATTGGAGATGATCAAATTTATA ATGTAATTGTAACTGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGTGGATTTGGAAATTGA TTAGTGCCTTTAATATTAGGTGCTCCTGATATAGCATTCCCACGAATAAATAATATAAGATTTTGACTTCTACCTCCTGC TCTTTCTTTACTATTAGTAAGTAGAATAGTTGAAAATGGAGCTGGGACAGGATGAACTGTTTATCCACCTCTATCCGCTG GAATTGCTCATGGTGGAGCTTCAGTTGATTTAGCTATTTTTTCTCTACATTTAGCAGGAATTTCTTCAATTTTAGGAGCT GTAAATTTTATTACAACTGTAATTAATATACGATCAACAGGAATTTCATTAGATCGTATACCTTTATTTGTTTGATCAGT AGTTATTACTGCTTTATTATTATTATTATCACTTCCAGTACTAGCAGGAGCTATTACTATATTATTAACAGATCGAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Screenshot-2025-02-18-at-12.55.43 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_9923.heic,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_9922.heic,",42.4605100000,-73.3168000000,02/18/2025,"
$\"\"$ $\"\"$ $\"\"$ <\/a> $\"\"$ $\"\"$ $\"\"$ $\"\"$ <\/p>\n",Tenebrio molitor,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_0261.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_0298.jpg,","
Taq polymerase used: New England Biolabs One Taq Hot Start Quick-Load 2x MM w\/ Standard Buffer (product # M0488S)<\/p>\n
DNA Ladder used: New England Biolabs 1 kb Plus DNA<\/p>\n
Ladder for Safe Stains (product # N0559S)<\/p>\n
Single PCR W\/ Arthropod Primers 3\/4\/25 49c<\/p>\n
Duplex PCR W\/ Wolbachia Primers & Arthropod Primers 3\/25\/25 49c<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_7692-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_7689-scaled.jpeg,",42.3711377596,-73.3658557467,02/18/2025,"
Green Arachnid with a red dot at base of abdomen with white lines extending down abdomen.<\/p>\n",Tetragnatha viridis,Monarch DNA extraction (NEB),Whole arthropod,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_7913.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_8229-1.jpeg,","
For DNA extraction Lysis process lasted 35 minutes with vortexing solution every 7 minutes. Used insect adaptation of New England Biolabs Monarch spin gDNA extraction protocol. Used the Taq Master mix 2x. The Polymerase we used was NEB One Taq Hot start quick load 2x MM w\/ stand buffer (M0488s) we preformed two PCR\u2019s the first one on March 4th, 2025 for Arthropod CO1 primers and the second on March 25th, 2025 for Wolbachia 16s primers. We ran both PCR results through an Edvotek electrophoresis set to 125v and 0.2 amps current, for 25 minutes. Then utilized a blue box to read the results.<\/p>\n",No,NNNNNNNNNNTNTNNNNNNTTTNNCCGGACNTGTAGGTGCATTATATTTCAGTTATTTTTCGGGTTGAACTAGCACTACC CGGTGGGCATCTTGTNGATGATCATTTACATAATGTCAATGTAACAGCTCNTGCTTTTGCAATAATTATTGTAATTTTCA TGATTATTCCAACTGTGGGGTTTGGAAATGGAAAATACCCTTTACCATTTTTAGCCCCTGCCCCGGATTTTCCTTTTCNN AAGCAATATAAATTTTNTTTTTGACCCCCTGCCCCTGCTTTTATATTAATCTCGCCGTCAGGTTAGGTTGAGGTAGGAAC TGGTACAGCTGGCACACCGCATCCACCTCCAATTAATCGACTACGGCGTCCAGCGGCTGATTTGGATATTTTTATTCTAC NCTTACATCGTACTTGGCTTTCCTCCATTCCTGNTTCATTTATTACNACTGTCACTATCATNCNTATAAGAACGGTTGCT ATGGAGTTATTCCAATTGCNNNTATGNGCTGNGTCAATTNNGGTTACTGNCTTCTTGCTAACACTACCCATTCCAGTGTG AGCGAGTACNATACCGATGTCACAGACNGATCNAAATTTTNTNNTNNTNATCTCACGCGNGAAGGGGACCCANATCCANT GCTACNTCAGCATTNATTTTGAGNTCNNNNNACNNTNAANTNNNN,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_9356-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_9360-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_9353-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_9357-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_9359-scaled.jpeg,",42.4801752000,-73.1605217000,02/06/2025,"
-Black beetle with a horizontal brown strip across the back where the Neck attaches to the Body.<\/p>\n",Dermestes lardarius,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/Gel-DNA-pic.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/WOLBACHIA-DNA.png,","
1.) I got my DNA through the protocol found in lab 2: DNA extraction. However, when I did my cell Lysis step, I left the test tube in the heat for 30 minutes, vortexing every 10 minutes.
\n2.) Next, I followed the protocol found in lab 3: PCR with Athropod primers. 3\/4\/25
\n3.) followed Lab:4 pt 1 Gel electrophoresis w\/ arthropod primers See gel image above.
\n4.) Followed lab 3 part 2: PCR round 2
\nFor this step, I used the NEB OneTaq hot start as my Taq polymerase. I also ran my Annealing temp at 55c on 3\/31\/25.
\n5.) On 4\/1\/25 I ran a single PCR reaction on my DNA. I used Lab 4 part 2 from the Wolbachia project. I used a gel electrophoresis system to move my DNA and check for Wolbachia DNA. I used a TAE buffer and a SYBR safe DNA stain. The polymerase used was: New England Biolabs OneTaq Hot start quick-load 2x MM w\/ Standard buffer (product # M0488s). The DNA ladder I used was: New England Biolabs 1 kb plus DNA ladder for safe stains (product #N0559s). I only did one PCR on 3\/31\/25 using the NEB OneTaq hot start as my Taq polymerase. I also ran my Annealing temp at 55c.<\/p>\n
Final:
\nThe leader Beetle was Wolbachia +
\nWith a high confidence level
\nI am highly confident because there were no smears throughout my entire process. My controls also had or didn’t have lines when necessary. Such as when looking for DNA in water none was present and while testing my positive arthropod control, arthropod was present.<\/p>\n",Yes,NNNNNNNNNNNNNNNGNCGNNGNNNNGNNNNNNNNNNNNNNNNNNNNNNCNTNNNGNNNANTANGNCCNCCCGCNTGAAT TATTGGTGANGANCTCANGTTGTACTTGTGNTNNNCNCGNNCNGNCNATGATAANTTTTTTTATAGACGAACTAANTTNG ATGGNNGGAGNAGGGGANNTGTNNGTCNCCNCNCATGAGTGANCGCCCGANACNNCCNNCCCNNANNAANNNNNATAANA TGATTCCACTANCCTCCCCTCCNTTTCTTATTGNTTTCTTGAACACTAGTCCANTAGCNGGGACGCGAGNNCNGNTGTCN ATCCTCCCCCNTNNNCATCGNNNNTCAGGACGNGCANNCANTNNCTTNNNTTNTCTTTTNTTTTCNNCCNNNNGNNNNNN NNNNNNNATTCTGNNANANGNTTTTTNTTTGTTNNNNTNNNCNTNNNNNTNNANNNNNNTAGNNCGNNGNNNNNNNNNNN CNTGNTNNNTNNTNNNACTGTGNNNNNNNNNNNNTTNNATNNTCNNCNNNNNNNGNNTNNNNNNNNNNNNNNNNCATNNN NNTNANTNNNNNNTNNTNNNTTNTTNNTTNNNNANTNNNNNNNNNNNNNNNNATNNNNTNNNNNNNTGNNNGNNNNNNNN NTNTNNNNNNTATNNNNANANNNNNNNNNNNTNNNNANNNTNNNTNNNNTNNTNNNANANTNNNNNNNNNNN,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/20250218_122004-1-scaled.jpg,",42.6828184000,-73.0840343000,02/01/2025,"
Spider was collected from the top left-hand corner of a sliding door in the back side of the house.<\/p>\n",Theridiidae,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/1000004184-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/1000004172-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/1000004185-scaled.jpg,","
Taq polymerase used: New England Biolabs One Taq hot Start Quick-Load 2x MM w\/Standard Buffer (product # M0488S)<\/p>\n
DNA Ladder Used: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product #N0559S)<\/p>\n
First single PCR ran on 3\/04\/2025 at an annealing temperature of 49 degrees Celsius with arthropod primers. The results were inconclusive as the Theridiidae sample did not present with any band but the controls did provide distinct bands as well as the Steatoda Triangulosa sample. No signs of contamination.<\/p>\n
Second Duplex PCR was ran on 4\/1\/2025 at an annealing temperature of 49 degrees Celsius with both arthropod and Wolbachia primers. Two bands were distinctively seen for each control sample at 400 and another above each at 700. Contamination was evident as the water control had a distinct band as well.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BT-19-scaled.jpg,",43.469549966937976,-111.95279813783073,08/30/2024,"",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,,Other,GelGreen,"","",No,GCTTGGTCTGCTATAGTTGGAACAGCTATAAGAGTTCTTATTCGAATTGAGTTAGGTCAACCTGGTAGATTTTTGGGTGATGATCATTTATATAATGTTATTGTTACTGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCAATCATGATTGGTGGGTTTGGTAATTGATTAGTTCCTTTAATATTGGGAGCTCCAGATATAGCTTTTCCTCGTATAAATAATTTAAGTTTTTGATTGTTACCGCCTTCTTTGATTATATTGTTTATTTCTTCTATAGTTGAGATAGGTGTTGGGGCGGGGTGAACTATTTATCCTCCTTTAGCTTCTTCTATTGGTCATTTTGGTAGATCTGTTGATTTTGCTATTTTTTCTTTGCATTTGGCTGGGGCTTCTTCTATTATAGGTGCTATTAATTTTATTTCTACTATTTTTAATATACGATCTGTTAGAATAACTATAGAGAAGGTTCCTTTATTTGTATGATCTGTTTTAATTACTGCTGTTTTGTTATTATTGTCTTTACCTGTATTAGCTGGTGCTATTACTATATTATTAACTGATCGAAATTTTAATACTTCTTTTTTTGATCCTGCTGGGGGAGGAGATCCTATTTTATTTCAGCA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BT-18-3-scaled.jpg,",43.47070221526693,-111.9532129310547,08/23/2024,"",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,,Other,GelGreen,"","",No,TGATCTGCTATAGTGGGGACTGCTATAAGAGTGTTAATTCGTTTAGAATTAGGAAATTCTGGAAGTTTATTAGGAGATGATCATTTATATAATGTTATAGTTACTGCTCATGCTTTTGTGATGATTTTTTTTATAGTAATGCCAATTCTTATTGGAGGTTTTGGTAATTGATTAATTCCTTTAATGTTAGGTGCTCCTGATATATCATTTCCTCGAATGAATAATCTTTCTTTTTGGTTATTGCCCCCTTCTTTATTTTTATTAACTATGTCTTCTATAGTGGAAATAGGGGTAGGAGCTGGTTGAACAGTTTATCCTCCTTTAGCATCTAGAATAGGTCATATAGGGAGTTCTATAGATTTTGCTATTTTTTCTCTTCATTTAGCTGGTGCTTCTTCTATTATAGGGGCAGTAAATTTTATTTCTACTATTATTAATATACGAATATTAGGTATATCTATAGATAAGGTACCTTTATTTGTTTGGTCAGTTTTGATTACGGCTGTTTTATTATTATTATCTTTACCTGTTTTAGCAGGTGCTATTACTATGTTATTAACTGATCGAAATTTTAATACTTCTTTTTTTGACCCTGCAGGGGGAGGAGATCCTATTTTATTTCAACATTTGTTTTGATTTTTTGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BT-17-2-scaled.jpg,",43.46857172571252,-111.95271531728518,08/20/2024,"",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,,Other,GelGreen,"","",No,GCTTGGTCTGCTATAGTTGGAACAGCTATAAGAGTTCTTATTCGAATTGAGTTAGGTCAACCTGGTAGATTTTTGGGTGATGATCATTTATATAATGTTATTGTTACTGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCAATCATGATTGGTGGGTTTGGTAATTGATTAGTTCCTTTAATATTGGGAGCTCCAGATATAGCTTTTCCTCGTATAAATAATTTAAGTTTTTGATTGTTACCGCCTTCTTTGATTATATTGTTTATTTCTTCTATAGTTGAGATAGGTGTTGGGGCGGGGTGAACTATTTATCCTCCTTTAGCTTCTTCTATTGGTCATTTTGGTAGATCTGTTGATTTTGCTATTTTTTCTTTGCATTTGGCTGGGGCTTCTTCTATTATAGGTGCTATTAATTTTATTTCTACTATTTTTAATATACGATCTGTTAGAATAACTATAGAGAAGGTTCCTTTATTTGTATGATCTGTTTTAATTACTGCTGTTTTGTTATTATTGTCTTTACCTGTATTAGCTGGTGCTATTACTATATTATTAACTGATCGAAATTTTAATACTTCTTTTTTTGATCCTGCTGGGGGAGGAGATC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BT-16-scaled.jpg,",43.468902631297844,-111.9516183654713,07/26/2024,"",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,Other,GelGreen,"","",No,CTTGGGCTGCTATAGTAGGAACAGCAATAAGAGTATTAATTCGTGTTGAGTTAGGTCAGACTGGAAGATTATTAGGGGATGATCAATTATATAATGTTATTGTTACGGCGCATGCTTTTATTATAATTTTTTTTATAGTAATACCTATTTTGATTGGTGGGTTTGGGAACTGATTAGTTCCTTTAATATTAGGGGCTCCTGATATAGCTTTTCCTCGAATAAATAATTTAAGATTTTGATTATTGCCTCCTTCTTTATTATTATTATTTATTTCTAGTATAGATGAGATAGGTGTTGGGGCTGGATGAACTATTTATCCTCCTTTAGCTTCTTTAGAGGGTCATTCTGGAAGTTCTGTAGATTTTGCAATTTTTTCTTTACATTTAGCTGGTGCTTCGTCTATTATAGGGGCTATTAATTTTATTTCTACAATTTTAAATATGCGTGGGTATGGAATATCTATAGAAAAAGTTCCTTTATTTGTCTGATCTGTTTTGATTACAGCTGTGTTGTTATTATTGTCTTTACCTGTTCTTGCAGGAGCTATTACTATGCTTTTAACTGATCGAAATTTTAACACCTCTTTTTTTGATCCTTCTGGGGGAGGTGACCCTGTATTATTTCAACATTTATTTTGA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BT-15-scaled.jpg,",43.4265851000,-111.9044611000,10/22/2024,"",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,Other,GelGreen,"","",No,GCTGCTATAGTGGGAACAGCAATAAGTGTATTAATTCGAATAGAGTTGGGACAAGTAGGTAAATTTTTAGGTGATGATCATTTATATAATGTAATTGTCACTGCTCATGCGTTTGTTATAATTTTTTTTATAGTTATACCTATTTTGATTGGTGGATTTGGAAATTGATTAGTTCCTTTAATACTTGGAGCTCCTGATATGGCATTTCCTCGTATAAATAATTTAAGTTTTTGATTATTACCACCTTCATTATTATTATTATTTATTTCATCGATAGTGGAAATGGGAGTAGGGGCAGGTTGAACTGTTTACCCTCCTTTAGCTTCTTTAATAGGCCATGCTGGAAGTGCTGTTGATTTTGCAATCTTTTCGTTACATTTAGCTGGGGCATCCTCAATTATAGGGGCTATTAATTTTATTTCTACTGTTATTAACATACGTTCAATTGGTATAAGTATAGAAAAAGTACCATTGTTTGTGTGATCAGTAGTAATCACAGCAGTATTATTATTATTATCTTTACCTGTTTTAGCTGGGGCTATTACAATATTGCTTACGGATCGTAATTTTAATACTTCTTTTTTTGACCCAGCTGGTGGTGGGGATCCTATTTTATTTCAGCATTTATTTTGATTTTTTGGT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BT-14.png,",43.469705573695265,-111.95012302626954,08/14/2024,"",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,,TAE,GelGreen,"","",No,ATAGTGGGGACTGCTATAAGAGTGTTAATTCGTTTAGAATTAGGAAATTCTGGAAGTTTATTAGGAGATGATCATTTATATAATGTTATAGTTACTGCTCATGCTTTTGTGATGATTTTTTTTATAGTAATGCCAATTCTTATTGGAGGTTTTGGTAATTGATTAATTCCTTTAATGTTAGGTGCTCCTGATATATNNTTTCCTCGAATGAATAATCTTTCTTTTTGGTTATTGCCCCCTTCTTTATTTTTATTAACTATGTCTTCTATAATGGAAATAGGGGTAGGAGCTGGTTGAACAGTTTATCCTCCTTTAGCATCTANAATAGGTCATATAGGGAGTTCTATAGATTTTGCTATTTTTTCTCTTCATTTANCTGGTGCTTCTTCTATTATAGGGGCAGTAAATTTTATTTCTACTATTATTAATATACGAATATTAGGTATATCTATAGATAAGGTACCTTTATTTGTTTGGTCAGTTTTGATTACGGCTGTTTTATTATTATTATCTTTACCTGTTTTAGCAGGTGCTATTACTATGTTATTAACTGATCAAAATTTTAATACTTCTTTTTTTGACCCTG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BT-13.png,",43.46898229840296,-111.95023840591158,08/13/2024,"",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,,TAE,GelGreen,"","",No,TATGATCTGCTATAGTGGGGACTGCTATAAGAGTGTTAATTCGTTTAGAATTAGGAAATTCTGGAAGTTTATTAGGAGATGATCATTTATATAATGTTATAGTTACTGCTCATGCTTTTGTGATGATTTTTTTTATAGTAATGCCAATTCTTATTGGAGGTTTTGGTAATTGATTAATTCCTTTAATGTTAGGTGCTCCTGATATATCATTTCCTCGAATGAATAATCTTTCTTTTTGGTTATTGCCCCCTTCTTTATTTTTATTAACTATGTCTTCTATAGTGGAAATAGGGGTAGGAGCTGGTTGAACAGTTTATCCTCCTTTAGCATCTAGAATAGGTCATATAGGGAGTTCTATAGATTTTGCTATTTTTTCTCTTCATTTAGCTGGTGCTTCTTCTATTATAGGGGCAGTAAATTTTATTTCTACTATTATTAATATACGAATATTAGGTATATCTATAGATAAGGTACCTTTATTTGTTTGGTCAGTTTTGATTACGGCTGTTTTATTATTATTATCTTTACCTGTTTTAGCAGGTGCTATTACTATGTTATTAACTGATCGAAATTTTAATACTTCTTTTTTTGACCCTGCAGGGGGAGGAGATCCTATTTTATTTCAACATTTGTTTTGATTTTTTGGTCA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_7010-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_7011-scaled.jpg,",44.9014259000,-93.2475232000,09/17/2024,"
I collected this ant outside on a sidewalk. It was around 5 pm but relatively warm out. There were a lot of other ants but this was the biggest one.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,GelGreen,"","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/LA6_ant-CO1F-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BT-12.png,",43.469260939838456,-111.95163633600723,07/15/2024,"",Arthropoda,Edwards Buffer,Abdomen,,TAE,GelGreen,"","",No,AGGGACTGCTATTGTGTGTTAATTCGGATGGAATTAGGAAATTCTGGAAGTTTATTAAGGGATGATCATTTATATAATGTTATANTTACTGCTCCCGCTTTAGTTAGGATTTTTTTTANGGTAATACCCATTCTTATTGGTGGATTTGGAAACTGATTGGTTCCTTTAACGTTAGGTGCTCCTGATATATCATTTCCTCTTATAAATAATCTTTTTTTTTGAATATTACTACCTTCTCTGTTTTTATTGTGAACATTATCAANNTTANAAATACGATTAGGGACTGGTTGAACTGTTTATCCTCCTTTGGCTTCTATAATTGGGCACATAGGGAGTTCTATGGATTTTGCTATTTTTTCTCTTNCCTTAGCTGGGGCTTCTTCACTTAGACCTGCACTAAATTTTAATTCNACAATTATTAATATACAAATATTATCAACCCCTATGGAAAATGTTCNTTTANTTGTTTGGTCATTATTAATTACTGCACNTTAATTATTACTTNCTTTCTACGTA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_7824-scaled.jpg,",44.9253811429,-93.1726140651,09/15/2024,"
Ant was living in a crack in the sidewalk. Collected on an abnormally hot and humid day.<\/p>\n",Insecta,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-01-110706.png,","
My data is lane 4.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_3495.jpg,",44.9691279000,-93.2878982000,09/21/2024,"
\n
8 legs (4 per side)<\/li>\n
large abdomen (stripey pattern)<\/li>\n
no antennae<\/li>\n
no wings<\/li>\n
2 pedipalps<\/li>\n<\/ul>\n",Araneae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,GelGreen,"","
\n
Use SeeGreen DNA dye in 1.5% agarose gel<\/li>\n
Submerge Gel in TBE buffer<\/li>\n
Use of Mini PCR Electrophoresis System<\/li>\n
5\u03bcl of DNA ladder<\/li>\n
2\u03bcl loading dye (SeeGreen) + 8\u03bcl DNA in each lane<\/li>\n
Positive control (arthropod positive for Wolbachia)<\/li>\n
Negative control (water)<\/li>\n<\/ul>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BT-10-cropped-1-scaled.jpg,",43.470631621302395,-111.94850655153893,,"",Arthropoda,Edwards Buffer,Abdomen,,TAE,GelGreen,"","",No,AGTGGGGACTGCTATAAGAGTGTTAATTCGTTTAGAATTAGGAAATTCTGGAAGTTTATTAGGAGATGATCATTTATATAATGTTATAGTTACTGCTCATGCTTTTGTGATGATTTTTTTTATAGTAATGCCAATTCTTATTGGAGGTTTTGGTAATTGATTAATTCCTTTAATGTTAGGTGCTCCTGATATATCATTTCCTCGAATGAATAATCTTTCTTTTTGGTTATTGCCCCCTTCTTTATTTTTATTAACTATGTCTTCTATAGTGGAAATAGGGGTAGGAGCTGGTTGAACAGTTTATCCTCCTTTAGCATCTAGAATAGGTCATATAGGGAGTTCTATAGATTTTGCTATTTTTTCTCTTCATTTAGCTGGTGCTTCTTCTATTATAGGGGCAGTAAATTTTATTTCTACTATTATTAATATACGAATATTAGGTATATCTATAGATAAGGTACCTTTATTTGTTTGGTCAGTTTTGATTACGGCTGTTTTATTATTATTATCTTTACCTGTTTTAGCAGGTGCTATTACTATGTTATTAACTGATCGAAATTTTAATACTTCTTTTTTTGACCCTGCAGGGGGAGGAGATCCTATTTTATTTCAACATTTGTTTTGATTTTTTGGTCACCC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_1438-scaled.jpg,",45.0816621618,-93.0958758665,09/15/2024,"
It was an ant with a three-part body where each section had an equal size. It has six legs and antennae but no wings.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SeeGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-01-110417.png,","
In the gel image screenshot, my arthropod is lane 5.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BT-9-scaled.jpg,",43.46995473562893,-111.94977970351563,07/30/2024,"",Arthropoda,Edwards Buffer,Abdomen,,TAE,GelGreen,"","",No,TTAAATGTTATAGTTACTGCTCAAGCTTTTGTGATGATTTTTTTTATAAAAATGCCAATTCTTATTGGAGGTTTTGGTAATTGATTAATTCCTTTAATGTTAGGTGCTCCTGATATATCATTTCCCCAAAAGAATAATCTTTCTTTTTGGTTATTGCCCCCTTCCTTATTTTTATTAACTATGTCTTCTATANTGAAAAAAGGGGTAGGAGCTGGTTGAACANTTTATCCTCCTTTAGCATCTANAATAGGTCATATAGGGAGTTCTATANATTTTGCTATTTTTTCTCTTCCTTTNNCTGGTGCTTCNTCTATTATAGGGGCAGTAAATTTTANTTNTACTANTATTAATATACNAACATTNNGTATATCCANAGATAANGTACCTTTANTTGTTTGGTCANTTTTGATTACAGCTGTTTTATTAT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BT-8-scaled.jpg,",43.47027348345447,-111.95050264639056,07/30/2024,"",Arthropoda,Edwards Buffer,,,TAE,GelGreen,"","",No,AATTAGGAAATTCTGGAAGTTTATTAGGAGATGATCATTTATATAATGTTATAGTTACTGCTCATGCTTTTGTGATGATTTTTTTTATAGTAATGCCAATTCTTATTGGAGGTTTTGGTAATTGATTAATTCCTTTAATGTTAGGTGCTCCTGATATATCATTTCCTCGAATGAATAATCTTTCTTTTTGGTTATTGCCCCCTTCTTTATTTTTATTAACTATGTCTTCTATAGTGGAAATAGGGGTAGGAGCTGGTTGAACAGTTTATCCTCCTTTAGCATCTAGAATAGGTCATATAGGGAGTTCTATAGATTTTGCTATTTTTTCTCTTCATTTAGCTGGTGCTTCTTCTATTATAGGGGCAGTAAATTTTATTTCTACTATTATTAATATACGAATATTAGGTATATCTATAGATAAGGTACCTTTATTTGTTTGGTCAGTTTTGATTACGGCTGTTTTATTATTATTATCTTTACCTGTTTTAGCAGGTGCTATTACTATGTTATTAACTGATCGAAATTTTAATACTTCTTTTTTTGACCCTG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/BT-7-scaled.jpg,",43.46970897784078,-111.94282484371249,07/19/2024,"",Arthropoda,Edwards Buffer,Abdomen,,TAE,GelGreen,"","",No,GCTATAAGAGTTCTTATTCGAATTGAGTTAGGTCAACCTGGTAGATTTTTGGGTGATGATCATTTATATAATGTTATTGTTACTGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCAATCATGATTGGTGGGTTTGGTAATTGATTAGTTCCTTTAATATTGGGAGCTCCAGATATAGCTTTTCCTCGTATAAATAATTTAAGTTTTTGATTGTTACCGCCTTCTTTGATTATATTGTTTATTTCTTCTATAGTTGAGATAGGTGTTGGGGCGGGGTGAACTATTTATCCTCCTTTAGCTTCTTCTATTGGTCATTTTGGTAGATCTGTTGATTTTGCTATTTTTTCTTTGCATTTGGCTGGGGCTTCTTCTATTATAGGTGCTATTAATTTTATTTCTACTATTTTTAATATACGATCTGTTAGAATAACTATAGAGAAGGTTCCTTTATTTGTATGATCTGTTTTAATTACTGCTGTTTTGTTATTATTGTCTTTACCTGTATTAGCTGGTGCTATTACTATATTATTAACTGATCGAAATTTTAATACTTCTTTTTTTGATCCTGCTGGGGGAGGAGATCCTATTTTATTTCAGCATTTATTTTGATTTTTTGGTCACCC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_2002-rotated.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_2001-rotated.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_1999-rotated.jpg,",44.9250632659,-93.1750009024,09/20/2024,"
Caught on a very large cottonwood tree next to the soccer field. It was walking very slowly. It was caught in the fall when the temp outside was 70 degrees (F). It has two wings, six legs, and a black body, and it’s covered in small hairs. It also has big eyes.<\/p>\n",,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,,GelGreen,"","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IB5_ant-CO1F-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_8719-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_3587-scaled.jpg,",44.9257561000,-93.1742021000,09/18/2024,"
It was warm, and 70 degrees f. The ant was found on the concrete but was surrounded by trees an mulch and a lot of grass.<\/p>\n",Formicidae,DNeasy (Qiagen) blood and tissue kit,,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_9308-1-scaled.jpg,","
High confidence in PCR.<\/p>\n",No,ATAAGAATACTTATTCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTAATTCTATAGTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCCTTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCTCCTTCAATCAGTTTACTTTTATTAAGAAATTTCATTAATGACGGAGTGGGGACAGGGTGAACTGTTTACCCTCCATTAGCTTCTAATATTTTTCATAATGGTCCTTCAGTTGACCTAACAATTTTTTCTCTACATATTGCAGGGATATCCTCTATTCTAGGAGCTATCAATTTTATTTCAACAATTTTAAATATGCGACATAAAAATTTTTCTCCTGATAAAATCCCTTTACTTGTTTGATCTATTTTAATTACAGCAATTTTACTTCTTCTTTCTTTACCAGTTCTAGCAGGAGCTATTACTATACTTTTAACTGACCGAAACCTTAATACTTCTTTTTTTGATCCCTCAGGANGGGGGGANCCTGTTCTTTATCAACATTTATTCTGA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/SR5_ant-CO1F-2.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_6968-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_6414-scaled.jpeg,",44.9516643000,-93.1629063000,09/16/2024,"
Ant was found on the sidewalk by my house. I collected it in the afternoon. It was 84 degrees. There were only a few ants around.<\/p>\n",Formicidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,GelGreen,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_1321-1-scaled.jpeg,",44.9330076000,-93.1629063000,09/22/2024,"
I found this ant on a wood fence very close to some soil\/woodchips, trees, and flowers. It was sunny and approximately 70 degrees farenheit. I had trouble finding other ants in the area.<\/p>\n",Formicidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_9308-1-1-scaled.jpg,","
samples were ran with positive wolbachia control and negative water control and DNA ladder which goes from 1200bp to 100bp with a bright spot at 700bp. on a piece of parafilm 8 micro liters of DNA and 2 micro liters of loading dye were mixed using a micropipette. The DNA sample was loaded into the gel. The gel was run for 20-30 minutes.<\/p>\n",No,NNNNNNNNNNNNNTCTGAGCTGGAATAATTGGATCTTCAATAAGAATACTTATTCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTAATTCTATAGTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCCTTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCTCCTTCAATCAGTTTACTTTTATTAAGAAATTTCATTAATGACGGAGTGGGGACAGGGTGAACTGTTTACCCTCCATTAGCTTCTAATATTTTTCATAATGGTCCTTCAGTTGACCTAACAATTTTTTCTCTACATATTGCAGGGATATCCTCTATTCTAGGAGCTATCAATTTTATTTCAACAATTTTAAATATGCGACATAAAAATTTTTCTCCTGATAAAATCCCTTTACTTGTTTGATCTATTTTAATTACAGCAATTTTACTTCTTCTTTCTTTACCAGTTCTAGCAGGAGCTATTACTATACTTTTAACTGACCGAAACCTTAATACTTCTTTTTTTGATCCCTCAGGAGGGGGGGACCCTGTTCTTTATCAACATTTATTCTGATTTTTTGGTCACCTGGAAANTTAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/EG5_ant-CO1F-2-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_3625-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_3624-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_3623-scaled.jpg,",44.9460643070,-93.1942484878,10/25/2024,"
Caught inside my house, it has 8 legs and a big sack in the rear, it was about 69 degrees inside my house, it was in a corner near a heat source, it was sitting on a cobweb.<\/p>\n",Steatoda triangulosa,DNeasy (Qiagen),Body without legs,MiniPCR,TBE,SeeGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/gel-electrophoresis.png,","",Yes,TTCGGGTAGAATTANGACAATCTGGAAGATTGTTAGGTGATGATCAATTATATAATGTAATTGTTACTGG ACACGCTTTTGTAATAATTTTTTTTATAGTAATACCANTATTAATTGGTGGATTTGGAAATTGGTTGGTT CCATTAATATTAGGGGCTCCTGATATAGCTTTTCCTCNAATAAATAATTTAAGATTTTGATTATTACCTC CTTCTTTATTATTATTATTAATTTCTTCTATANAANAAATANGTGTTGGAGCTGGNTGAACTATTTACCC CCCTTTATCTTCTCTGGAAGGTCATANAGGAAGATCTGTANATTTTGCTATNNTNNNTTTACNTTTGGCN GGNNNTTCNTCNATTATAGGTGCTATTAATTTTATTTCTACTATTTTAAATATACGTTTATTTGGGATGA GAATAGAAAAAGTTANACTTTTTGTTTGGTCAGTATTAATCACTGCAGTANTATTATTATTATCNTNACC TGTATTGNCNGG,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/CG5_spider-Good-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_2425-scaled.jpg,",44.9250253842,-93.1742453500,09/18/2024,"
Small, black, ant-looking, has 6 legs and three segments(head, abdomen, head), most likely has antennas but is a bit crushed. The bug has no wings. Caught in the SPA courtyard during fall, it was about 70 degrees F. The courtyard is grassy and open.<\/p>\n",Tetramorium forceps,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,SeeGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_2435-scaled.jpg,","
My results were in lane 5.<\/p>\n",No,NNNNNNTAAGTATAATTATTCGACTAGAATTAGGATCATGTATTTCTTTAATTAATAATGATCAAATTTATAATATCCTA GTAACAAGCCNTGCTTTTATTTTAATTTTTTTTATACTAATGCTTTTTATAATCGGGGGATTTGGAAATTTTTTATTTCC TTTAATACTAGGAACTCCAGATATACCTTACCCCAAAAAAAAAAAAAAAAAATTTGACCTCCCCCCCCATCTATTAAAAT TATTTTAGTTAAAAAATTTTATAATTCCGGGGTTGGGATGGGGNAACTATCCACCCTCCCCTACTACCTATTTTTCCTCA GGGACCATCAATTACCCTTCAAATTTTTTCCCCTNTTTTGCTGAAAATTTTTTCTTTTTAAGAGACAAAAATTTTTTTTT TTCCCTCCTCAATTAACCCCATTTAAAACTAAACCTACGT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/RG5_ant-CO1F-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_0008-scaled.jpeg,",44.7629378116,-93.4804170757,09/17/2024,"
Small arthropod with many legs. The top of the arthropod was a shell I found it next to a tree and it was about 72 degrees F outside<\/p>\n",Armadillidae,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/unnamed-scaled.jpg,",44.9095374000,-93.1679257000,09/19/2024,"
It was caught in the fall inside a house; it was on the wall. it was around 4 pm and it was 75\u00b0f. It was alone<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/Screenshot-2025-03-31-132246.png,","
DNA in sixth lane<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/SH3_ladybug-CO1F-1-big-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_8814-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_7858-scaled.jpg,",44.9606790025,-93.1230168766,01/21/2025,"
The bug was found in the dirt under a rock at 9:32 AM. It was early fall and relatively cold outside.<\/p>\n",Armadillidium,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,GelGreen,"","",Yes,TACAGAGTTAGGACAACCTGGTAGGTTAATCGGTGATGACCAGATTTATAATGTAATTGTAACTGCGCATGCTTTCGTGA TAATTTTTTTnAnnnnAATGCCCATTATAATTGGGGGTnnnGGAAATTGACTCnTACCTTTAATGTTAGGAGCTCCTGAT ATAGCTTTCCCTCGTATAAATAATATGAnGTTTTGGTTGTTACCCCCTTCTTTGACTTTACTTCTTAGTAGCGGATTAAT TGAAAGAGGGGTTGGAACCGGGTGAACGGTTTACCCGCCCCTTGCCAGCGGAATCGCTCATAGGGGGGCTTCnnTAnATT TAGGAATTnnnTCGCTTCATTTAnnnGnnnnTTCTTCGATTTTAGGGGCTGTAAATTTTATTACCACTGTAATTAATATG CGAGCTCCGGGGnTTnAAATAnACCGAGTACnnnnnTTTGTTTGGTCTGTCTTGATTACAGCTGTTCTTTTATTACTTTC TCTGCCTGTTTTAGCAnGGGCAATTACAATATTAnTAACCGATCGAAATTnnAnTACTTCTTTTTTTGATCnnnGCGGAG nnnGnnAnCCTATTCTTTAC,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/RH3_rolypoly-CO1F-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_20250214_090929187-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_20250214_091205809_HDR-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_20250214_091241097_HDR2.jpg,",42.4185257732,-72.6306328740,02/13/2025,"
Spider found indoors in a bathroom.<\/p>\n
Link to observation on iNaturalist: https:\/\/www.inaturalist.org\/observations\/261630285<\/p>\n",Pholcus,Monarch DNA extraction (NEB),Whole arthropod,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/PCRwithArthropodPrimers.png,","",,,,, "",44.9541079914,-93.0864460313,09/23/2024,"
Caught in the fall on a tree. It is black. It was mainly in the bark.<\/p>\n",Hymenoptera,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"","",Yes,NGGCTGTCGTNNGCTCGTGTCGTGAGANGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGG TAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTAT GGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCA TCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGA ATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTT,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/RR3_rolypoly-CO1F-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/image0.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/image0-1-scaled.jpeg,",44.9257561000,-93.1742021000,09/17/2024,"
It was caught in the fall under a rock. The rock had a wet area and cooler temperature, as well as dirt and mud surrounding it. The bug was black and curled up when it was found. It was caught on September 17th, 2024, in the summer, and it was 73 degrees Fahrenheit. The habitat was urban.<\/p>\n",Isopoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,GelGreen,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_1890-scaled.jpg,",44.9531827382,-93.0898040042,09/19/2024,"
Found this Ant in St. Paul on September 19 of 2024. Temperature was mid 70 roughly 75\/76. Arthropod is very small and was found in a ant colony of similar arthropods.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,GelGreen,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_8317-scaled.jpg,",44.9260662163,-93.1728675070,09/19/2024,"
The arthropod was collected during Autumn at 22 degrees Celsius in dry dirt adjacent to the pavement. The arthropod has six legs, three body segments, and antennae.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,GelGreen,"","
Control is located in lane 8, DNA is located in lane 4<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/PG3_ant-CO1F-1-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_1154.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_1153.jpg,",44.9260092174,-93.1734860076,09/18/2024,"
Small, black-ish brown at with six legs, two long antennae, two pincers, and a large head and thorax. Found on sidewalk in an urban environment in autumn, 77 degrees Fahrenheit. Crawling around on dry leaves, moving slowly on its own, no other ants in sight.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,GelGreen,"","
Ant DNA = Lane 2<\/strong><\/p>\n
DNA Ladder = Lane 1<\/strong><\/p>\n
DNA Positive Control = Lane 8<\/strong><\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/CD3_ant-CO1F-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/ant-scaled.jpg,",44.9254852971,-93.1745591238,09/20/2024,"
The arthropod was caught in St.Paul during mid-September.<\/p>\n",Hymenoptera,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"","",No,TTGGNNCTTCNATAAGAATACTTATTCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTAAT TCTATAGTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTTTT AGTTCCTTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCTCCTTCAA TCAGTTTACTTTTATTAAGAAATTTCATTAATGACGGAGTGGGGACAGGGTGAACTGTTTACCCTCCATTAGCTTCTAAT ATTTTTCATAATGGTCCTTCAGTTGACCTAACAATTTTTTCTCTACATATTGCAGGGATATCCTCTATTCTAGGAGCTAT CAATTTTATTTCAACAATTTTAAATATGCGACATAAAAATTTTTCTCCTGATAAAATCCCTTTACTTGTTTGATCTATTT TAATTACAGCAATTTTACTTCTTCTTTCTTTACCAGTTCTAGCAGGAGCTATTACTATACTTTTAACTGACCGAAACCTT AATACTTCTTTTTTTGATCCCTCANGANGGGGGGANCCTGTTCTTTATCAACATTNATTCTGANTTTTTGNTCACC,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/KM3_ant-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_1512-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_1513-1-scaled.jpg,",44.9330076000,-93.1629063000,09/19/2024,"
Caught in late summer\/ early fall. It was warm outside.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,GelGreen,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-10-153644.png,",44.9224589028,-93.1785720455,09/12/2024,"
Small arthropod identified as an “Armadillidium vulgare,” a.k.a. a “Roly-Poly.” The subject’s habitat was a moist dirt patch in a grass yard, although it was photographed in a plastic tube. The “Armadillidium vulgare” has a brown shell back,14 small white legs, and two antennas.<\/p>\n",Isopoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/download-scaled.jpg,","
I dropped the reproductive parts in the wrong tube before PCR and had to extract it out which may have lead to faulty data<\/p>\n",Unknown,N/A,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_9817-scaled.jpg,",44.9541888218,-93.0947643186,09/12/2024,"
\n
Small in size<\/li>\n
Redish dark brown<\/li>\n
6 legs<\/li>\n
Head\n
\n
Antennae<\/li>\n
Mandibles<\/li>\n<\/ul>\n<\/li>\n
Thorax\n
\n
Smaller then abdomen<\/li>\n<\/ul>\n<\/li>\n
Abdomen\n
\n
Largest part<\/li>\n
Is dark brown with light brown\/red stripes<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/wolbachia.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_4070-scaled.jpeg,",44.94659248693609,-93.08103554065588,09/28/2024,"
on food plate outside 50 degrees F.<\/p>\n",Insecta,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,SeeGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/Screenshot-2025-03-31-194946.png,","
observe lane 3<\/p>\n",Yes,NNNNNNNNNNNNNNNNGCTATTTGAGCAGGAATAATTGGATCTTCTATAAGTATAATTATTCGTTTAGAATTAGGTTCGTCTAATTCATTAATCAATAATGATCAAATTTATAATTCTTTAGTAACTAATCACGCTTTTATTATAATTTTTTTCATAGTAATACCATTTATAATTGGTGGATTTGGAAATTTTCTAATTCCTTTAATACTAGGATCACCAGACATAGCATACCCTCGTATAAATAATATAAGATTTTGACTCTTACCTCCTTCAATTACTCTTTTACTTTTAAGAAATTTTATTAATGATGGAACAGGAACAGGATGGACTATTTATCCTCCCTTATCCTCTAATATTTTTCATAATGGTCCTTCTGTAGACTTAACAATTTTTTCTCTTCATATTGCAGGTATATCCTCAATTTTAGGAGCAATTAATTTTATTTCAACAATTCTTAATATACATCATAAAAATTTTTCTATTGATAAAATTCCTTTACTCGTATGATCAATTTTAATTACAGCTATCTTACTTCTATTATCCTTACCTGTATTGGCCGGAGCTATTACTATACTATTAACTGATCGAAATTTAAATACTTCATTCTTTGATCCTTCGGGGGGAGGTGATCCAATTCTATATCAACATTTATTTTGATTTTTTGGTCACCCNNNNAAGTTTAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/AN1_ant-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_1172-scaled.jpg,",44.9664244271,-93.0870807144,09/09/2024,"
This cicada was found lying on its back on concrete in front of my school. It was 84 degrees out and sunny. it was 6:00 pm when I found it. It was alone and was flapping its wings on the ground.<\/p>\n",Magicicada,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,MiniPCR,TBE,GelGreen,"","",No,NA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_4070-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_4072-scaled.jpeg,",44.9252801078,-93.1742339755,09/28/2024,"
On stairs outdoors, surrounding food left outside, with others of its own, fall, 50 degrees F<\/p>\n",Insecta,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,SeeGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/Screenshot-2025-03-31-091913.png,","
Lane 4 is the observed arthropod.<\/p>\n",Yes,CTTCTATAAGnATAATTATTCGTTTAGAATTAGGTTCGTCTAATTCATTAATCAATAATGATCAAATTTATAATTCTTTAGTAACTAATCACGCTTTTATTATAATTTTTTTCATAGTAATACCATTTATAATTGGTGGATTTGGAAATTTTCTAATTCCTTTAATACTAGGATCACCAGACATAGCATACCCTCGTATAAATAATATAAGATTTTGACTCTTACCTCCTTCAATTACTCTTTTACTTTTAAGAAATTTTATTAATGATGGAACAGGAACAGGATGGACTATTTATCCTCCCTTATCCTCTAATATTTTTCATAATGGTCCTTCTGTAGACTTAACAATTTTTTCTCTTCATATTGCAnGnATATCCTCAATTTTAGGAGCAATTAATTTTATTTCAACAATTCTTAATATACATCATAAAAATTTTTCTATTGATAAAATTCCTTTACTCGTATGATCAATTTTAATTACAGCTATCTTACTTCTATTATCCTTACCTGTATTGGCCGGAGCTATTACTATACTATTAACTGATCGAAATTTAAATACTTCATTCTTTGATCCTTCGGGGGGAGGTGATCCAATTCTATATCAACATTTA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IS1_ant-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/99F2E694-106C-488F-A3CB-83EF9BE65539-scaled.jpeg,",44.9516643000,-93.1629063000,10/13/2024,"
I found this medium-sized and oval-shaped bug with lots of legs under a piece of cement in my backyard. I collected it in the fall, in the evening, and the temperature was 52\u00b0F.<\/p>\n",Isopoda,DNeasy (Qiagen) blood and tissue kit,Rear half,MiniPCR,TBE,GelGreen,"","",Unknown,N/A,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/Ophyiulus-Pilosus-scaled.jpeg,",25.9397412000,-81.7075355000,01/02/2025,"
In landscape.<\/p>\n",Diplopoda,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Wolbachia-Gel.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/6DB38D85-373A-4FC6-B2A5-F7ACCC135FA0-scaled.jpeg,",25.8728864000,-81.6692532000,01/12/2025,"
In kitchen<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_8449-scaled.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_4617-scaled.jpeg,",25.8728864000,-81.6692532000,01/02/2025,"
In the landscape<\/p>\n",Coenagrionidae,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/Screen-Shot-2025-03-27-at-4.44.28-PM.png,","
Lane 1= Ladder<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/01C2CE5C-88CC-4E11-9AF6-F0C3EB1B565B-scaled.jpeg,",40.5591664315,-75.4025246525,02/20/2025,"
In window sill<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_6101-1-scaled.jpeg,","
1- Water(-)<\/p>\n
2- DNA Ladder<\/p>\n
3- Arthropod 1<\/p>\n
4- Arthropod 2<\/p>\n
5- Arthropod(+)<\/p>\n
6- Arthropod(-)<\/p>\n
7- DNA(+)<\/p>\n
8- Water(-)<\/p>\n
9- BLANK<\/p>\n
10- DNA Ladder<\/p>\n
11- Wolbachia 1<\/p>\n
12- Wolbachia 2<\/p>\n
13- Wolbachia(+)<\/p>\n
14- Wolbachia(-)<\/p>\n
15-DNA(+)<\/p>\n",No,NNNNNTTTTCGGAGCTGATCAGGAATAGTAGGAACCTCTCTAAGAATTTTAATTCGAGCAGAACTAGGACATCCTGGAGC ATTAATTGGCGATGATCAAATTTATAATGTAATTGTAACAGCTCATGCCTTTGTTATAATTTTTTTTATAGTAATACCTA TTATAATTGGAGGATTTGGAAATTGACTTGTCCCTTTAATACTAGGAGCACCTGATATAGCATTTCCTCGAATGAATAAT ATAAGTTTTTGACTTTTACCTCCTGCACTTACACTACTATTGGTGAGCAGTATAGTGGAAAACGGAGCTGGGACAGGATG AACTGTTTACCCTCCTCTATCTTCTAATATTGCTCATGGAGGAGCTTCTGTTGATTTAGCTATCTTTTCCCTTCATTTAG CAGGAATTTCTTCAATTTTAGGAGCAGTTAATTTTATTACAACTGTTATTAATATGCGATCTACAGGAATTACATTTGAC CGAATACCTTTATTTGTTTGATCTGTAGTAATTACAGCATTATTACTCCTTTTGTCCTTACCTGTATTAGCAGGAGCTAT TACTATATTATTAACAGATCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGAGGAGGTGATCCAATTTTATACCAAC ATTTATTTTGATTTTTTGGTCACCCNGAAGTTTAAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_3647-scaled.jpeg,",25.8728864000,-81.6692532000,,"
In a garage<\/p>\n",Arthropoda,DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL,Abdomen,Standard electrophoresis system Bio-Rad,0.25 TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-02-at-3.03.44 PM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/8C214510-B353-4EC4-A4E6-C93DE33E2441-scaled.jpeg,",25.8477916975,-81.6820264550,01/02/2025,"",Insecta,Blood and Tissue kit,Partial abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_2341.jpeg,","
Lane<\/p>\n
1- DNA ladder<\/p>\n
2- Arthropod one (Ghost spider)<\/p>\n
3- Arthropod two (grain moth)<\/p>\n
4- Drosophila Positive<\/p>\n
5- Drosophila Negative<\/p>\n
6- DNA control<\/p>\n
7- water<\/p>\n
8 Blank<\/p>\n
9- DNA ladder<\/p>\n
10- Arthropod one (Ghost Spider)<\/p>\n
11- Arthropod two (Grain moth)<\/p>\n
12- Wolbachia positive control<\/p>\n
13- Wolbachia Negative control<\/p>\n
14- DNA control<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_2168-scaled.jpeg,",25.8728864000,-81.6692532000,01/02/2025,"",Gelechiidae,Blood and Tissue kit,Partial abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_2341.jpeg,","
Lane<\/p>\n
1- DNA ladder<\/p>\n
2- Arthropod one (Ghost spider)<\/p>\n
3- Arthropod two (grain moth)<\/p>\n
4- Drosophila Positive<\/p>\n
5- Drosophila Negative<\/p>\n
6- DNA control<\/p>\n
7- water<\/p>\n
8 Blank<\/p>\n
9- DNA ladder<\/p>\n
10- Arthropod one (Ghost Spider)<\/p>\n
11- Arthropod two (Grain moth)<\/p>\n
12- Wolbachia positive control<\/p>\n
13- Wolbachia Negative control<\/p>\n
14- DNA control<\/p>\n",No,NNNNNNNNNNNNNNNGCAGGTATAGTAGGAACATCTTTAAGATTATTAATTCGTGCTGAATTAGGAAATCCTGGATCATT AATTGGTGATGATCAAATTTATAATACTATTGTAACAGCTCATGCTTTCATTATAATTTTTTTTATAGTTATACCTATTA TAATTGGAGGATTTGGAAATTGATTAGTTCCTCTTATATTAGGAGCTCCAGATATAGCTTTCCCTCGAATAAACAATATA AGATTTTGACTCCTTCCCCCTTCTATTATACTTTTAATTTCAAGTAGAATTGTAGAAAATGGAGCAGGTACAGGATGAAC AGTTTACCCCCCACTTTCATCTAATATTGCTCATAGAGGAAGATCAGTAGATTTAGCAATCTTTTCTCTTCATTTAGCCG GAATTTCATCAATTTTAGGAGCTGTAAATTTTATTACAACTATTATTAATATACGCCCTAATAATATATCCTTAGATCAA ATACCCCTATTTGTTTGATCTGTAGGAATTACAGCTTTATTATTACTTTTATCTCTACCTGTATTAGCAGGTGCAATTAC CATATTATTAACAGATCGAAATTTAAATACATCATTTTTTGATCCTGCAGGAGGAGGTGACCCCATTCTTTATCAACATT TATTTTGATTTTTTNGGTCACCCTGAANTTTAANN,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_5148-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_5153-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_5155-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_5156-scaled.jpeg,",42.4500845000,-73.2453824000,02/20/2025,"
Specimen (ELMS2) was found by active collection inside a home in Pittsfield, Massachusetts. It was spotted in the ceiling corners of a foyer on February 20th of 2025 at approximately 7:00 A.M. EST. ELMS2 was collected by hand into a collection tube. Specimen was captured alive and placed in a freezer for 15 minutes, then transferred to Berkshire Community College to be stored in the freezer again at 9:30 A.M. EST. By 11:00 A.M. EST., the specimen was removed from the freezer and photographed with a cellular device on separate petri dishes through a digital screen on a dissecting scope at 8x magnification. After all photographs were taken, ELMS2 was placed into a 2 ml tube labeled accordingly and filled with isopropyl alcohol. ELMS2 was then finally stored in a 0 degrees celsius freezer for preservation until the DNA extraction.<\/p>\n",Pholcus phalangioides,Monarch DNA extraction (NEB),Whole arthropod,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Single-PCR-Arthropod-CO1-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Single-PCR-Wolbachia-16S.jpg,","
A single PCR\u00a0(ELMSPCR1) was done on ELMS1 and ELMS2 (separate data entry for specimen ELMS2 but all testing, extractions, and PCRs were done together) in order to find the Arthropod Barcoding Gene (CO1) on March 26, 2025. The expected band size was 708bp with an annealing temperature of 49 degrees celsius. The taq polymerase used was NEB OneTaq Hot Start Quick-Load 2x MM w\/ Stand. Buffer (M0488S). The DNA Ladder used was NEB 1 kb Plus DNA Ladder for Safe Stains (product # N0559S). Primes used to amplify a 708-bp fragment of the CO1 gene were Arthropod_F (LCO1490) and Arthropod_R (HCO2198). Images of the PCR are attached above labeled, “Single PCR Arthropod CO1 (ELMSPCR1).”<\/p>\n
A single PCR\u00a0(ELMSPCR2) was done on ELMS1 and ELMS2 (separate data entry for specimen ELMS2 but all testing, extractions, and PCRs were done together) in order to find the Wolbachia Specific Gene (16S rRNA) on March 27, 2025. The expected band size was 438bp with an annealing temperature of 55 degrees celsius. The taq polymerase used was NEB OneTaq Hot Start Quick-Load 2x MM w\/ Stand. Buffer (M0488S). The DNA Ladder used was NEB 1 kb Plus DNA Ladder for Safe Stains (product # N0559S). Primers to specifically amplify a 438-bp fragment of the 16S rRNA gene were Wolbachia_F (16S_WspecF) and Wolbachia_R (16S_WspecR). Images of the PCR are attached above labeled, “Single PCR Wolbachia 16S (ELMSPCR2).”<\/p>\n",No,NNNNNNNNNNNNNNNTGNNNCNNNNNNATTAGGTACTGCTTTAAGAGTGATTATTCGAGTGGAGTTGACGACAAGGAGGA AGATTTTGANGGGATGATCATTTATATAGTGTTGTANTAACTGCTCATGCTTTAATTATGATTTTTTTTATAGTTATGCC TATTTNAATTGGGGGGTTTGGAAATTGATTAGTTCCTTTAATGTTGGGGGCTCCNGATATAGCTTTTCCTCGTATAAATA ATTTNAGATTTTGGCTTCCTCCTCCTGCTATTATGCTTTTATTATTATCTGGTCTTGTAAAAACTGGAGTTGGAGCAGGA TGAACTATTTATCCTCCTTTATCTTCTAGAGTAGGGCATANAGGGGTTTCCATATATTTTGCTATTTTTTCTCTTCATTT AGCGGGGGCTTCATCTATTATAGGAGCTATTAATTTTATTTCTACNGTTGTTAATATACNATTAATTGGAGTGANAATGG ATAAATTGAGATTGTTTGTTTGATCTGTATNNATTACTGCTGTTCNTTNACTTCTTTCNTNACCTGTTGNACCTGGTGCT ATNACTATACTTTTGACTGATCGGANTTCTAATACTGCGNTTNTTTGACCCNGCNGGAGGAGGGGANCCAATCTTGTTTC CACTTCTTTNTNGATNTNNNNGTCACCCTGAANNTNAAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_5132-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_5133-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_5137-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_5139-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_5130-scaled.jpeg,",42.4500845000,-73.2453824000,02/20/2025,"
\n
\n
\n
\n
Specimen was found by active collection inside a home in Pittsfield, Massachusetts. It was spotted in the ceiling corners of a foyer on February 20th of 2025 at approximately 7:00 A.M. EST. ELMS1 was collected by hand into a collection tube. Specimen was captured alive and placed in a freezer for 15 minutes, then transferred to Berkshire Community College to be stored in the freezer again at 9:30 A.M. EST. By 11:00 A.M. EST., the specimen was removed from the freezer and photographed with a cellular device on a petri dish through a digital screen on a dissecting scope at 8x magnification. After all photographs were taken, ELMS1 was placed into a 2 ml tube labeled accordingly and filled with isopropyl alcohol. ELMS1 was then finally stored in a 0 degrees celsius freezer for preservation until the DNA extraction.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n",Pholcus phalangioides,Monarch DNA extraction (NEB),Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_5541-1.jpg,","
A single PCR\u00a0(ELMSPCR1) was done on ELMS1 and ELMS2 (separate data entry for specimen ELMS2 but all testing, extractions, and PCRs were done together) in order to find the Arthropod Barcoding Gene (CO1) on March 26, 2025. The expected band size was 708bp with an annealing temperature of 49 degrees celsius. The taq polymerase used was NEB OneTaq Hot Start Quick-Load 2x MM w\/ Stand. Buffer (M0488S). The DNA Ladder used was NEB 1 kb Plus DNA Ladder for Safe Stains (product # N0559S). Primes used to amplify a 708-bp fragment of the CO1 gene were Arthropod_F (LCO1490) and Arthropod_R (HCO2198). Images of the PCR are attached above labeled, “Single PCR Arthropod CO1 (ELMSPCR1).”<\/p>\n
A single PCR\u00a0(ELMSPCR2) was done on ELMS1 and ELMS2 (separate data entry for specimen ELMS2 but all testing, extractions, and PCRs were done together) in order to find the Wolbachia Specific Gene (16S rRNA) on March 27, 2025. The expected band size was 438bp with an annealing temperature of 55 degrees celsius. The taq polymerase used was NEB OneTaq Hot Start Quick-Load 2x MM w\/ Stand. Buffer (M0488S). The DNA Ladder used was NEB 1 kb Plus DNA Ladder for Safe Stains (product # N0559S). Primers to specifically amplify a 438-bp fragment of the 16S rRNA gene were Wolbachia_F (16S_WspecF) and Wolbachia_R (16S_WspecR). Images of the PCR are attached above labeled, “Single PCR Wolbachia 16S (ELMSPCR2).”<\/p>\n",No,NNNTTNNNANNNNNNATCTNCTATAGTAGGTACTGCTTTAAGAGTGATTATTCNAGTGGAGTTAGGACAAGGAGGAAGAT TTTTAGGGGATGATCATTTATATAATGTTGTAGTAACTGCTCATGCGTTAATTATGATTTTTTTTATAGTTATGCCTATT TTAATTGGGGGGTTTGGAAATTGATTAGTTCCTTTAATGTTGGGGGCTCCAGATATAGCTTTTCCTCGTATAAATAATTT AAGATTTTGGCTTCTTCCTCCTGCTATTATGCTTTTATTATTATCTGGTCTTGTAAAAACTGGAGTTGGAGCAGGATGAA CTATTTATCCTCCTTTATCATCTANAGTAGGGCATAGAGGGGTTTCCATAGATTTTGCTATTTTTTCTCTTCATTTAGCG GGGGCTTCANCNATTATAGGAGCTATTAATTTTATTTCTACAGTTGTTAATATACGATTAATTGGAGTGAGAATGGATAA AGTGAGATTGTTTGTTTGATCTGTATTAATTACTGCTGTTCTTTTACTTCTTTCTTTACCTGTTTTAGCTGGTGCTATTA NNATACNTTTGACTGATCGNAATTTTAATACTGCCNTTTTTTTGACCCTGCTGGAGGANGGGATCCAATCTTGNTTCNAC ATCTTTTTTCNGANTTTTTTGNTNNNCCCTGAAATTTNAANNN,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_5126.jpeg,",40.5591757759,-75.4025537039,02/20/2025,"
Found in window sill<\/p>\n",Coccinellidae,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_6101-scaled.jpeg,","
1- Water(-)<\/p>\n
2- DNA Ladder<\/p>\n
3- Arthropod 1<\/p>\n
4- Arthropod 2<\/p>\n
5- Arthropod(+)<\/p>\n
6- Arthropod(-)<\/p>\n
7- DNA(+)<\/p>\n
8- Water(-)<\/p>\n
9- BLANK<\/p>\n
10- DNA Ladder<\/p>\n
11- Wolbachia 1<\/p>\n
12- Wolbachia 2<\/p>\n
13- Wolbachia(+)<\/p>\n
14- Wolbachia(-)<\/p>\n
15-DNA(+)<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_4618-scaled.jpeg,",25.8728864000,-81.6692532000,01/02/2025,"
In the landscape<\/p>\n",Diplopoda,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/Screen-Shot-2025-03-27-at-4.44.28-PM-1.png,","
Lane 1=Ladder<\/p>\n
La<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/BE485412-6108-466A-BBE1-F8FF2567388F-scaled.jpeg,",25.8728864000,-81.6692532000,01/12/2025,"
In the landscape<\/p>\n",,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_2195-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3131.jpeg,","
\n
<\/li>\n<\/ol>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_0929-scaled.jpeg,",25.8728864000,-81.6692532000,01/02/2025,"
In the landscape<\/p>\n",Diplopoda,DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL,Partial abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/Picture1.png,","
$\"Gel$ <\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_6006-scaled.jpeg,",25.8728864000,-81.6692532000,01/02/2025,"
In a garage<\/p>\n",Blattodea,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_6131-scaled.jpeg,","
$\"\"$ $\"\"$ <\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/ant.jpg,",1.4257977000,103.7755000000,02/28/2025,"
It is hairy, has characteristics of an arthropod<\/p>\n",Arthropoda,,,,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/Screenshot-2025-03-14-at-2.11.25-PM.png,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/beetle.jpg,",1.4257977000,103.7755000000,,"
The beetle wouldn’t die. very small.<\/p>\n",Arthropoda,,,,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/Screenshot-2025-03-14-at-2.11.25-PM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Screenshot-2025-02-28-at-3.21.04 PM.png,",1.3520830000,103.8198360000,02/28/2025,"
6 legs, a hard casing covering the thorax and abdomen, a head, antennae, and pinchers at its end. hairs on its legs.<\/p>\n",Dermaptera,,Abdomen,,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/Screenshot-2025-03-14-at-2.08.08 PM.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Screenshot-2025-02-28-at-3.20.55 PM.png,",1.3520830000,103.8198360000,02/28/2025,"
a6 legs, a head, a thorax, and abdomen. hairs on legs, head, and abdomen.<\/p>\n",Monomorium pharaonis,,Whole arthropod,,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/Screenshot-2025-03-14-at-2.13.29 PM.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_2687-scaled.jpg,",1.4257977000,103.7755000000,02/28/2025,"
Small, black, dead beetle with visible head.<\/p>\n",Coleoptera,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/Screenshot-2025-03-14-at-2.10.38 PM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_2195-scaled.jpg,",1.4263517000,103.7731691000,02/28/2025,"",Arthropoda,DNeasy (Qiagen),Abdomen,MiniOne,lysis,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_2709-scaled.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_2200-scaled.jpg,",1.4257977,103.7755,02/28/2025,"
The abdomen was cut off<\/p>\n",Arthropoda,DNeasy (Qiagen),Whole arthropod,MiniOne,lysis,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_2709-scaled.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_8308-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_8307-scaled.jpeg,",42.4603382511,-73.3168299657,02/18/2025,"",Drosophila melanogaster,Monarch DNA extraction (NEB),Whole arthropod,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/1D9393AC-25CB-4E27-9074-F046F5DD4D9A.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/172F31FC-7D01-4B44-9D33-183DE14A47C9.jpeg,","
Taq Polymerase used: NEB OneTaq Hot Start Quick-Load 2x MM w\/ Standard Buffer (product # M0488S)<\/p>\n
DNA Ladder: NEB 1kb Plus DNA Ladder for Safe Strains (product # N0559S)<\/p>\n
3\/4\/25 conducted single arthropod PCR<\/p>\n
Primers: Arthropod_F (LCO1490), Arthropod_R (HCO2198)<\/p>\n
Annealing temp 49\u00b0C<\/p>\n
3\/25\/25 conducted single Wolbachia PCR<\/p>\n
Primers: Wolbachia_F (16S_WspecF), Wolbachia_R (16S_WspecR)<\/p>\n
Annealing temp 55\u00b0C<\/p>\n",Yes,AGTTGGAACATCTTTAAGAATTTTAATTCGAGCTGAATTAGGACATCCTGGAGCATTAATTGGAGATGATCAAATTTATA ATGTAATTGTAACTGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGTGGATTTGGAAATTGA TTAGTGCCTTTAATATTAGGTGCTCCTGATATAGCATTCCCACGAATAAATAATATAAGATTTTGACTTCTACCTCCTGC TCTTTCTTTACTATTAGTAAGTAGAATAGTTGAAAATGGAGCTGGGACAGGATGAACTGTTTATCCACCTCTATCCGCTG GAATTGCTCATGGTGGAGCTTCAGTTGATTTAGCTATTTTTTCTCTACATTTAGCAGGAATTTCTTCAATTTTAGGAGCT GTAAATTTTATTACAACTGTAATTAATATACGATCAACAGGAATTTCATTAGATCGTATACCTTTATTTGTTTGATCAGT AGTTATTACTGCTTTATTATTATTATTATCACTTCCAGTACTAGCAGGAGCTATTACTATATTATTAACAGATCGAAATT TAAATAC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_3570-rotated.jpg,",44.9255636574,-93.1748188426,09/19/2024,"
Black ant, with some lines on the third body section.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_3583-rotated.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/LT1_ant-CO1F-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_8616-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_8618-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_8607-1-scaled.jpg,",42.4500845000,-73.2453824000,02/19/2025,"
Tan colored insect that is approximately 1 inch in length.\u00a0 It has antenna, legs and they hold their wings flat over their backs since they no longer fly.\u00a0 These are typically sold in local pet stores to feed pets like lizards.\u00a0 I purchased this cricket at PetCo in Pittsfield, MA.<\/p>\n",Acheta domesticus,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_8845.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_8912.jpg,","
Taq Polymerase (New England Biolabs One Taq Hot Start) used, Start Quick-Load 2x MM w\/ Standard Buffer (product #M0488S.<\/p>\n
DNA Ladder Used: New England Biolabs 1kb Plus DNA Ladder for Safe Stains (product #N0559S)<\/p>\n
Annealing temperatures 1 cycle 30 sec @ 94 C, 30 cycles 30 sec @ 94 C and 45 sec @ 49 C and 60 Sec @ 68 C, 1 cycle 5 min @ 68 C.\u00a0 Arthropod PCR reaction completed on 3\/13\/25, annealing temperature 49C.\u00a0 Wolbachia (single) PCR reaction completed on 4\/3\/25, annealing temperature 55C.<\/p>\n",No,CTCTTTAAGTATCTTAATTCGAACGGAACTAGGACAACCAGGTTATTTAATTGGAGATGATCAAACATATAATGTTATCG TAACTGCACATGCATTTGTCATAATTTTTTTCATGGTTATACCAATTATAATTGGTGGATTCGGAAATTGATTAGTACCC CTAATATTAGGTGCACCCGATATAGCCTTTCCTCGAATAAACAATATAAGATTTTGACTTTTACCACCCTCACTAACCCT TTTATTAACCAGAAGAATAGTCGAAAATGGTGCAGGGACAGGATGAACAGTTTATCCACCTTTATCAACAGGAATCGCCC ACGCCGGAGCATCTGTTGATTTAGCCATTTTTTCATTACACTTAGCTGGAATTTCATCAATTCTGGGAGCCGTTAATTTC ATTACAACTATAATCAATATACGAGCACCTGGGATGTCATTAGATCAAACCCCATTATTTGTATGAGCTGTTGGAATCAC TGCCCTCCTCCTATTATTATCTTTACCTGTTCTTGCGGGTGCAATCACAATATTACTAACAGATCGAAATCTGAATACAT CATT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/4A0218A0-31E2-4B7D-8B12-DD75B9CDE464-scaled.jpeg,",25.8728864,-81.6692532,01/02/2025,"",,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_6173-scaled.jpeg,","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/37A1A9E3-93EA-44D1-82FE-BE7B297B477D-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_6466-scaled.jpeg,",40.5472763000,-75.4169918000,12/01/2024,"",Vespidae,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/Screenshot-2025-03-26-at-4.37.24 PM.png,","
The PCR protocal was optimized according to barcoding gene and wolbachia. The 1st group was heated for optmized results for the barcoding gene and 2nd group was heated for optmized results for wolbachia<\/p>\n",No,NNNNCTTTGNTTTATGAGCTGGTATAATTGGAAGATCATTAAGATTAATTATTCGTCTAGAATTAGGTACACCTTTATCA ATTATTAATAATGATCAAATATACAATTCAATTATTACAGCTCACGCATTAATTATAATTTTTTTCATAGTTATACCATT TATAATCGGAGGATTTGGAAATTGATTAATTCCCTTAATATTAGGAGCCCCAGATATAGCATTCCCACGAATAAATAATA TAAGATTTTGATTATTACCACCATCATTATTATTATTAATCATTAGAAATATTAATGATATAGGAGTAGGAACTGGATGA ACTTTATATCCACCTTTATCATCTAATATAGGACACAATTCATCATCAATTGATTTTGCTATTTTCTCTCTTCACATCGC AGGAATTTCATCAATTATAGGAGCTATTAATTTCATTGTAACAATCTTAAATATACATATTAAAACTCATTCATTAAATT TTTTACCTATATTTACATGATCAGTTTTAATTACTGCAATTTTACTTTTATTATCCTTACCTATTCTAGCCGGAGCAATT ACCATACTATTAACTGATCGAAATATTAATACATCTTTTTTTGATCCATTAGGAGGTGGAGATCCAATTTTATTCCAACA TTTATTTTGATTTTTTGGTCACCNNNNNAAGTTTAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_6471-scaled.jpeg,",40.5472763000,-75.4169918000,12/01/2024,"
Caught in the classroom<\/p>\n
<\/p>\n",Halyomorpha halys,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system Bio-Rad,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/Screenshot-2025-03-26-at-4.37.24 PM.png,","
The PCR protocal was optimized according to barcoding gene and wolbachia. The 1st group was heated for optmized results for the barcoding gene and 2nd group was heated for optmized results for wolbachia.<\/p>\n",No,NNNNNTNNCTTCGGATATGATCTGGAATAGTAGGATCAGCTATAAGATTAATTATCCGTATCGAATTAGGACAGCCTGGA AGATTTATTGGTAATGATCAAATTTATAATGTAATTGTAACAGCACATGCATTTGTAATAATTTTCTTTATAGTAATACC AATCATAATTGGAGGATTCGGTAATTGATTAGTCCCTTTAATAATTGGAGCACCTGATATAGCCTTCCCACGATTAAATA ATATAAGATTCTGATTATTACCCCCTTCATTAACTTTATTATTAATAAGAAGAATAGCAGAATCAGGAGCTGGGACAGGA TGAACAGTCTATCCCCCCTTATCAAGTAATATCTCACATAGAGGAGCATCAGTTGATTTAGCTATTTTTTCCTTACATTT GGCTGGGGTTTCATCAATTTTAGGGGCAGTAAATTTTATTTCAACTATTATAAATATACGACCAACAGGTATAACCCCTG AACGAATCCCATTGTTTGTGTGATCAGTAGGAATTACTGCACTTCTTCTGTTATTATCCTTACCTGTGTTAGCAGGTGCT ATTACTATACTATTAACTGACCGAAATTTTAATACCTCTTTTTTTGACCCATCAGGAGGAGGAGACCCTATTCTTTATCA ACATTTATTTTGATTTTTTGGTCACCCNGGAAGTTNAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/20250219_151427-scaled.jpg,",25.8728864000,-81.6692532000,01/02/2025,"
Found in the landscape<\/p>\n",Harpalus pensylvanicus,DNeasy (Qiagen),Partial abdomen,Standard electrophoresis system Bio-Rad,TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Wolbachia-Gel-Electrophoresis.jpg,","",Yes,TTTTTTNNNNNGNNTGAGCNNGAATAGTAGGAACTTCATTAAGTATATTAATTCGAACTGAATTGGGGACTCCTGGTGCA TTGATTGGTAATGATCAAATTTATAATGTTATTGTTACTGCTCATGCTTTCGTAATAATTTTTTTTATAGTTATACCTAT TATAATTGGAGGATTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGCCTTTCCTCGAATAAATAACA TAAGTTTTTGATTACTTCCTCCTTCTTTAACTCTTCTCTTAATGAGAAGTATAGTGGAAAGAGGAGCTGGTACTGGATGA ACAGTTTACCCACCTTTATCATCTAGAATTGCTCATAGAGGAGCTTCAGTAGATTTAGCAATTTTTAGATTACATTTAGC TGGAATTTCATCAATTTTAGGTGCAGTAAACTTTATTACTACCATTATTAATATGCGATCAGTAGGAATAACATTTGATC GAATACCATTATTTGTATGATCAGTAGGAATTACAGCATTACTTTTACTTTTATCATTACCAGTATTAGCAGGAGCTATT ACTATATTACTTACAGATCGAAATTTAAATACTTCATTTTTTGATCCTGCCGGAGGAGGAGATCCTATTTTATACCAACA TTTATTTTGATTTTTTGGTCACCCNNGAAAGTTTAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_3648-scaled.jpeg,",25.8728864000,-81.6692532000,,"
In a garage<\/p>\n",Blattodea,DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL,Abdomen,Standard electrophoresis system Bio-Rad,0.25 TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-02-at-3.03.44 PM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_0511.png,",25.9397412000,-81.7075355000,01/02/2025,"
Found on the beach.<\/p>\n",Dermaptera,Blood and Tissue kit,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Arthopod-Labeling-.png,","
<\/p><\/blockquote>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/unnamed.jpg,",1.4262051545,103.7735615331,02/28/2025,"
Roach with missing legs<\/p>\n",Periplaneta americana,DNeasy (Qiagen),Abdomen,MiniOne,lysis,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/electrophoresis-ting.jpg,","",No,,,, "",1.4262362949,103.7736091134,02/28/2025,"
$\"Educational\"$ <\/p>\n",Monomorium pharaonis,none,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_2671-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/wolbachia-results.png,",1.3520830000,103.8198360000,02/28/2025,"
It’s a big ant, around 1-2mm in length<\/p>\n",Formicidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniOne,TBE,Other,"","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_4301-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_4302-scaled.jpg,",1.4268397668,103.7747834383,02/28/2025,"
beetle:<\/p>\n
The beetle has a thorax, abdomen and head.<\/p>\n
It’s very small in size<\/p>\n
ant:<\/p>\n
The ant has a thorax, abdomen and head.<\/p>\n
Its slightly bigger than the beetle<\/p>\n
its abdomen and thorax are larger.<\/p>\n",Arthropoda,,,MiniOne,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_4366-scaled.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_20250220_112311534-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_20250220_112258900-scaled.jpg,",42.2439762560,-73.1357300000,02/17/2025,"
Crayfish was collected from a stream in the forest during the winter.<\/p>\n",Cambaridae,Monarch DNA extraction (NEB),Partial abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2026/02/crayfish_gel1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2026/02/crayfish_gel2_duplex.png,","
\n
Taq Polymerase<\/strong> Used –\u00a0<\/strong>New England Biolabs OneTaq Hot Start Quick-Load 2x MM w\/ Standard Buffer (product #M0488S)<\/li>\n
DNA Ladder Used – <\/strong>New England Biolabs 1kb Plus DNA Ladder for Safe Stains (product #N0559S)<\/li>\n<\/ul>\n
Single Arthropod PCR set up on March 6th, 2025 to amplify fragment of the CO1 cytochrome oxidase gene using Arthropod_F (LCO1490) and Arthropod_R (HCO2198) Primers with an annealing temperature of 49\u00b0<\/span>C.<\/p>\n
Separate Single Wolbachia PCR set up on March 27th, 2025 to amplify fragment of the 16S ribosomal RNA gene using Wolbachia_F (16S_WspecF) and Wolbachia_R (16S_WspecR) Primers with an annealing temperature of 55\u00b0<\/span>C.<\/p>\n",No,TTCATTAAGGTTAATTATTCGAGTTGAATTAGGCCAGGTAGGTAGATTGATTGGAGACGACCAGATTTATAATGTGGTAG TAACAGCTCATGCTTTTGTTATAATTTTTTTTATAGTAATACCAATTATGATTGGAGGGTTTGGAAATTGGTTAATTCCT TTGATGCTTGGGGCTCCGGATATAGCTTTTCCTCGAATAAATAATATAAGATTTTGGTTGCTTCCTTTTTCTCTGACATT ATTATTAGCTAGGGGTATAGTAGAGAGGGGAGTGGGTACTGGGTGAACTGTTTATCCCCCTCTTGCTTCAGCAATCGCTC ATGCGGGTGCATCAGTAGATCTTGGAATTTTTTCTTTACATTTGGCTGGAGTATCCTCTATTTTAGGGTCAGTTAATTTT ATAACAACAGCTATCAATATACGGATGGTTGGTATAACTATGGATCGTATACCGTTATTTGTTTGATCTGTATTTATTAC TACTATTTTATTATTATTGTCTTTGCCTGTATTAGCAGGGGCAATC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_2706-1.jpg,",19.5159563135,-155.0114853802,02/04/2025,"
Six ants (A1-A6) and one mosquito (M1) were collected and assessed. Presence of Wolbachia was observed in all six ants while no presence of WalB was indicated in mosquito collected. All controls worked accordingly.<\/p>\n",Ochetellus glaber,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_349EF9DF9396-1-1.jpeg,","
Six ants scraped off the side of home in Aina loa, Hawai’i at 7:30am on a sunny morning. M1 mosquito was collected on 1\/31\/25 at 6:30 am inside home of Aina loa, Hawai’i and was 68 degrees outside. PCR preparation and standard Gel electrophoresis prepared and ran. Two PCR tests examined including Co1 and WalB primers.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_2660.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/tempImagez9vqKJ-scaled.jpg,",42.4500845000,-73.2453824000,02/18/2025,"
The crickets lived in a box in Petco altogether.<\/p>\n",Acheta domesticus,Monarch DNA extraction (NEB),Rear half,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/26D1EE31-1734-4D68-9FAF-1DA48B8F6C1B.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/F752B747-50F3-4793-B8F4-978896146C67.jpg,","
For DNA extraction we could have incubated for longer than we did. Incubation time was 1 minute and we didn’t set an exact timer. Other than that, the protocol was followed.<\/p>\n
The Taq polymerase used were the New England Biolabs One Taq Hot Start. For PCR\u00a0with the arthropod primers the annealing temp was 49C.<\/p>\n
Updated:\u00a0<\/strong>For the duplex PCR the annealing temp was 49C.<\/p>\n
The Taq polymerase used-NEB OneTaq Hot Start Quick-Load 2x MM w\/stand.<\/p>\n
Date we set up second PCR reaction is 3\/26\/25.<\/p>\n
Updated 4\/2\/25: Taq polymerase used: <\/strong>New England Biolabs OneTaq Hot Start Quick-Load 2x MM w\/ Standard Buffer<\/p>\n
DNA Ladder Used:\u00a0<\/strong>New England Biolabs 1 kb Plus DNA Ladder for Safe Stains<\/p>\n",No,,,, "",42.4600498213,-73.3157096850,04/06/2025,"",Acheta domesticus,Monarch DNA extraction (NEB),Rear half,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/8A9F7B67-7855-4E4B-B716-BD0E37E9E50C.jpeg,","
Taq Polymerase used: New England Biolabs OneTaq Hot Start Quick-Load 2x MM w\/ Standard Buffer (product #M0488S)<\/p>\n
DNA Ladder used: New England BioLabs 1 kb Plus DNA Ladder for Safe Stains (product #N0559S)<\/p>\n
PCR date 4\/16\/25- Annealing temp 49C<\/p>\n",Yes,AGTAGGTACCTCTTTAAGTATCTTAATTCGAACGGAACTAGGACAACCAGGTTATTTAATTGGAGATGATCAAACATATA ATGTTATCGTAACTGCACATGCATTTGTCATAATTTTTTTCATGGTTATACCAATTATAATTGGTGGATTCGGAAATTGA TTAGTACCCCTAATATTAGGTGCACCCGATATAGCCTTTCCTCGAATAAACAATATAAGATTTTGACTTTTACCACCCTC ACTAACCCTTTTATTAACCAGAAGAATAGTCGAAAATGGTGCAGGGACAGGATGAACAGTTTATCCACCTTTATCAACAG GAATCGCCCACGCCGGAGCATCTGTTGATTTAGCCATTTTTTCATTACACTTAGCTGGAATTTCATCAATTCTGGGAGCC GTTAATTTCATTACAACTATAATCAATATACGAGCACCTGGGATGTCATTAGATCAAACCCCATTATTTGTATGAGCTGT TGGAATCACTGCCCTCCTCCTATTATTATCTTTACCTGTTCTTGCGGGTGCAATCACAATATTACTAACAGATCGAAATC TGAATACATCATTTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_8396.jpg,",42.4514620000,-73.2707820000,02/18/2025,"",Acheta domesticus,Monarch DNA extraction (NEB),Rear half,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_8481.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_8459.jpg,","
For DNA the incubation time (1 minute) with the elution buffer could have been surpassed or underestimated. No timer was set, we based our time off of how long other students let their DNA sit for. Other than that we followed the directions\/protocol the whole way through.<\/p>\n
Taq polymerase used were the New England Biolabs OneTaq Hot Start. For the PCR with the arthropod Primers the annealing temperature was 49 C.<\/p>\n
The Taq polymerase that were used was the New England Bioloabs OneTaq Hot Start Quick-Load 2x Mm w\/ Standard Buffer (product #M0488s). The DNA Ladder that was used was the New England Biolabs 1kb Plus DNA Ladder for Safe Stain (product #N0559S). This was our second attempt to get a clear stain from the arthropods, the date the duplex PCR was set up was March 26, 2025, the annealing temperature was 49 degrees Celsius.<\/p>\n",No,NNNNNNNNNNNNNNNGTTNTTCNTTTGNCTGTCTCNNGCTCTTTGTCANANANNNTTGGTTTAATTCCCCCAACNACCGC ANCCTTNTTCCTTATTTACCATCNGGTAANGCTGGGAACTTNAAGGAAACTGCCANTGATANCTGAANAANGGTGGGNNG ANTGTCNTNNCANCTGGCNNTTNNNGGATTGNGCTNCCNCNTGCTACAATGGTGGCTACAANGGGCTGCAAATTCGCAAG GCTTANCTAANNCTNNAAANCCATCTCATTTCGAANTGNACTCTGCCCTCNAGTGCATGAATTTGGAATCNCNAGAANNN TGGAATCACCACCCCACGNNAAATACTTTCTCGGGTCTGGTACACCCTGCCCGTCACCCCTGGGAAATTGTTNNNNNTTA ANNCNNNN,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_0184.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_0185.jpg,",42.4602843200,-73.3140278715,02/19/2025,"
Pill bug<\/p>\n
Hard exterior<\/p>\n
Lots of legs<\/p>\n
<\/p>\n",Armadillidium,Monarch DNA extraction (NEB),Whole arthropod,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Lane-1-DNA-Ladder.png,","
Taq Polymerase used: NEB OneTaq Hot Start<\/p>\n
PCR Done on 4\/4\/25<\/p>\n
Annealing temp: 49 degrees celsius<\/p>\n
DNA Ladder used: NEB 1kb Plus DNA Ladder<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_0871-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_0870-1-scaled.jpeg,",42.6911144454,-73.1058475359,02/05/2025,"
Multi-colored, black white brown, and also it is a little bit hairy.<\/p>\n",Araneidae,Monarch DNA extraction (NEB),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/original-A53F77BF-96C7-431A-8050-472FE10BCCFD.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_1620.jpeg,","
We used the NEB extraction kit to safely extract the DNA from our samples and our controls, and that we incubated for 1 hour instead of the recommending 2-3 hours. Used the Taq Polymerse used, and annealing temperatures for each PCR reaction. Taq polymerase used was the NEB one Taq Hot Start Quick load 2x MM u\/stand buffer, annealing temperature is 49 C for both of the PCR’s, since they were done on a duplex.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/tempImagerPIt9S-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/tempImagesxv583-scaled.jpg,",42.4668290000,-73.2034660000,03/29/2025,"
It is Juvenile, it has 6 legs, 2 antennas. It was collected in a captive colony, the borders of it are dark. Abdomen has some dark spots.<\/p>\n",Blaptica dubia,Monarch DNA extraction (NEB),Abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Image-4-3-25-at-1.29 PM.jpg,","
Taq Polymerase used: New England Biolabs OneTaq Hot Start Quick-Load 2x MM 2\/ Standard Buffer (product # M0488S)<\/p>\n
DNA Ladder used: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S)<\/p>\n
<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/tempImageXXmRnx-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/tempImageG03D7u-scaled.jpg,",42.4669567781,-73.2014114279,02/12/2025,"
This arthropod was collected in Petco(a local pet store & captive colony).<\/p>\n",Blaptica dubia,Monarch DNA extraction (NEB),Partial abdomen,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/IMG_3517-2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_2747.jpg,","
For DNA extraction we used the insect adaptation of the protocol.<\/p>\n
March 4th, 2025:<\/p>\n
Single PCR with Arthropod primers.<\/p>\n
Taq Polymerase used: New England Biolabs OneTaq.<\/p>\n
Annealing temperature for PCR reaction: 49 C.<\/p>\n
March 25th, 2025:<\/p>\n
Duplex PCR with Arthropod and Wolbachia primers.<\/p>\n
Taq polymerase used: NEB OneTaq Hot Start Quick-Load 2x MM w\/ Stand Buffer (M0244S).<\/p>\n
Annealing temperature for PCR reaction: 49 C.<\/p>\n
April 11, 2025:<\/p>\n
Running gel electrophoresis once again to look at results of March 25th’s Duplex PCR.<\/p>\n
Taq Polymerase used: New England Biolabs OneTaq Hot Start Quick-Load 2x MM w\/ Standard Buffer (product # M0488S).<\/p>\n
DNA Ladder used: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S).<\/p>\n
<\/p>\n
<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_4606-scaled.jpg,",42.4603735018,-73.3168634114,02/18/2025,"
This Fruit Fly was a part of a captive colony on the Berkshire Community College Campus. It has big red eyes, three pairs of walking legs, and one pair of wings for flying.<\/p>\n",Diptera,Monarch DNA extraction (NEB),Whole arthropod,Edvotek Gel Electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5062.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5063.jpg,","
We followed the DNA extraction protocol with no issues. The 1.5ml tubes were incubated for 37 minutes. We followed the insect adaptation of the protocol. On March 6th 2025, we set up for a PCR reaction using arthropod primers. Taq Polymerase used: New England Biolabs OneTaq Hot start. Our samples were ran in the thermal cycler: 1 cycle for 30 seconds at 94C, 30 cycles for 30 seconds at 94C, 30 cycles for 45 seconds at 49C, 30 cycles for 60 seconds at 68C, 1 cycle for 5 minutes at 68C, and held indefinitely at 4C. On March 25th 2025, we set up for another PCR reaction, this time using Wolbachia primers. We followed the same protocol as with the arthropod primers, the one difference being, the annealing temperature was 55C this time. We used the NEB OneTaq Hot Start DNA Polymerase, and held indefinitely at 4C.<\/p>\n",Yes,AGTTGGAACATCTTTAAGAATTTTAATTCGAGCTGAATTAGGACATCCTGGAGCATTAATTGGAGATGATCAAATTTATA ATGTAATTGTAACTGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGTGGATTTGGAAATTGA TTAGTGCCTTTAATATTAGGTGCTCCTGATATAGCATTCCCACGAATAAATAATATAAGATTTTGACTTCTACCTCCTGC TCTTTCTTTACTATTAGTAAGTAGAATAGTTGAAAATGGAGCTGGGACAGGATGAACTGTTTATCCACCTCTATCCGCTG GAATTGCTCATGGTGGAGCTTCAGTTGATTTAGCTATTTTTTCTCTACATTTAGCAGGAATTTCTTCAATTTTAGGAGCT GTAAATTTTATTACAACTGTAATTAATATACGATCAACAGGAATTTCATTAGATCGTATACCTTTATTTGTTTGATCAGT AGTTATTACTGCTTTATTATTATTATTATCACTTCCAGTACTAGCAGGAGCTATTACTATATTATTAACAGATCGAAATT TAAATACATCATTTTTT,,, "",34.5859314780,-111.4963322628,02/14/2025,"
The exact origin of this insect is unknown due to the fact that it was purchased online. More research to be done.<\/p>\n",Brachinus nigriventris,DNeasy (Qiagen),Abdomen,MiniPCR,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/MTSEF-Gels-4-1.png,","
Shards of abdomen were present in the sample, leading to smears in the gel.<\/p>\n",Unknown,,,, "",34.0489281000,-111.0937311000,02/14/2025,"
Specimen acquired through bugsincyberspace.com, so the exact location of origin is unknown.<\/p>\n",Gyrinus,DNeasy (Qiagen),Whole arthropod,MiniPCR,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/MTSEF-Gels-3.png,","
The gel shows smudges from fractured arthropod fragments left over from cell lysis.<\/p>\n",Unknown,,,, "",34.0489281000,-111.0937311000,02/14/2025,"
Specimen acquired through bugsincyberspace.com, so the exact location of origin is unknown.<\/p>\n",Gyrinus,DNeasy (Qiagen),Whole arthropod,MiniPCR,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/MTSEF-Gels-3.png,","
Arthropod debris was present in the sample after DNA purification, leading to arthropod fragments and smudges in the gel.<\/p>\n",Unknown,,,, "",34.0489281000,-111.0937311000,02/14/2025,"
Specimen acquired through bugsincyberspace.com, so the exact location of origin is unknown.<\/p>\n",Gyrinus,DNeasy (Qiagen),Whole arthropod,MiniPCR,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/MTSEF-Gels-3.png,","
Arthropod debris was present in the sample after DNA purification, leading to arthropod fragments and smudges in the gel.<\/p>\n",Unknown,,,, "",34.0489281000,-111.0937311000,02/14/2025,"
Specimen acquired through bugsincyberspace.com, so the exact location of origin is unknown.<\/p>\n",Gyrinus,DNeasy (Qiagen),Whole arthropod,MiniPCR,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/MTSEF-Gels-3.png,","
Arthropod debris was present in the sample after DNA purification, leading to arthropod fragments and smudges in the gel.<\/p>\n",Unknown,,,, "",34.0489281000,-111.0937311000,02/14/2025,"
Specimen acquired through bugsincyberspace.com, so the exact location of origin is unknown.<\/p>\n",Gyrinus,DNeasy (Qiagen),Whole arthropod,MiniPCR,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/MTSEF-Gels-3.png,","
Arthropod debris was present in the sample after DNA purification, leading to arthropod fragments and smudges in the gel.<\/p>\n",Unknown,,,, "",34.0489281000,-111.0937311000,02/14/2025,"
Specimen acquired through bugsincyberspace.com, so the exact location of origin is unknown.<\/p>\n",Gyna centurio,DNeasy (Qiagen),Abdomen,MiniPCR,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/MTSEF-Gels-2.png,","
Smudges are present on the gel, likely caused from arthropod debris in the sample.<\/p>\n",Yes,,,, "",34.0489281000,-111.0937311000,02/14/2025,"
Specimen acquired through bugsincyberspace.com, so the exact location of origin is unknown.<\/p>\n",Gyna centurio,DNeasy (Qiagen),Abdomen,MiniPCR,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/MTSEF-Gels-2.png,","
Arthropod debris was present in the sample after DNA purification, leading to arthropod fragments and smudges in the gel.<\/p>\n",Unknown,,,, "",34.0489281000,-111.0937311000,02/14/2025,"
Specimen acquired through bugsincyberspace.com, so the exact location of origin is unknown.<\/p>\n",Gyna centurio,DNeasy (Qiagen),Abdomen,MiniPCR,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/MTSEF-Gels-2.png,","
Arthropod debris remained in the sample.<\/p>\n",Unknown,,,, "",34.0489281000,-111.0937311000,02/14/2025,"
Specimen acquired through bugsincyberspace.com, so the exact location of origin is unknown.<\/p>\n",Gyna centurio,DNeasy (Qiagen),Abdomen,MiniPCR,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/MTSEF-Gels-2.png,","
The samples were impure due to shell fragments in the sample.<\/p>\n",Unknown,,,, "",34.3250055080,-111.6484941516,02/14/2025,"
Specimen acquired through bugsincyberspace.com, so the exact location of origin is unknown.<\/p>\n",Gyna centurio,DNeasy (Qiagen),Partial abdomen,MiniPCR,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/05/MTSEF-Gels-2.png,","
Arthropod fragments remained in the centrifuge tube after cell lysis.<\/p>\n",Unknown,,,, "",34.6389013482,-111.4299189356,02/14/2025,"
Specimen acquired through bugsincyberspace.com, so the exact location of origin is unknown.<\/p>\n",,DNeasy (Qiagen),Abdomen,MiniPCR,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/MTSEF-Gels-4.png,","
Arthropod fragments remained in the spin tubes after cell lysis, resulting in smudges on the shell.<\/p>\n",Unknown,,,, "",34.6101833140,-111.5672270475,02/14/2025,"
Acquired through bugsincyberspace.com, so the exact location of origin is unknown.<\/p>\n",Brachinus nigriventris,DNeasy (Qiagen),Abdomen,MiniPCR,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/MTSEF-Gels-4-1.png,","
After DNA extraction, arthropod debris remained in the spin tubes. This caused smudges on the gel.<\/p>\n",Unknown,,,, "",34.3149313177,-111.6651681876,,"
Acquired online, so exact location of origin is unknown.<\/p>\n",Brachinus nigriventris,DNeasy (Qiagen),Abdomen,MiniPCR,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/MTSEF-Gels-4-1.png,","
Arthropod debris was present during DNA purification, leading to shell fragment in the sample and smudges on the gel.<\/p>\n",Unknown,,,, "",34.5614524584,-111.6876248559,02/14/2025,"
Specimen was acquired through Bugsincyberspace.com, so the exact place of origin is unknown.<\/p>\n",Brachinus nigriventris,DNeasy (Qiagen),Abdomen,MiniPCR,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/MTSEF-Gels-4-1.png,","
Arthropod debris was present during cell lysis, resulting in fragments of exoskeleton represented in the gel smudges.<\/p>\n",Unknown,,,, "",34.3273138789,-111.4297829544,02/14/2025,"
The insect was acquired via Bugsincyberspace.com, so we don’t know the exact location of origin.<\/p>\n",Brachinus nigriventris,DNeasy (Qiagen),Abdomen,MiniPCR,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/MTSEF-Gels-4-1.png,","
Bits of arthropod debris were present during DNA extraction, creating fragments of shells that made smudges on the gel.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/ic-scaled.jpg,",35.5336192000,-78.3253504000,01/08/2025,"
it might have been missing a leg<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_2788-scaled.jpg,","
We scooped out the abdomen of our spider, following the protocol to the best of our ability. The PCR products we had were not all the way full. The well for the negative control was contaminated with wolbachia, we’re not sure how. Either we switched the labels or accidentally used a pipette tip twice.<\/p>\n",No,,,, "",35.40690119363369,-82.50322508641197,04/04/2025,"
Triangular abdomen with mottled color, brown with white flecks.<\/p>\n",Hemiptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_2798-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_2787-scaled.jpg,","
Followed protocol, extracted DNA goop from abdomen of the sample of Halymorpha Halys. May have left exoskeleton in before the buffer AL was added.<\/p>\n",No,,,, "",35.40755244974506,-82.50212412193063,03/31/2025,"
Triangular abdomen with mottled color, brown with white flecks.<\/p>\n",Hemiptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniOne,TBE,GelGreen,"","
We removed the abdomen of our\u00a0Halymorpha halys,\u00a0<\/em>then placed it into a centrifuge tube. We ground it up before adding buffer ATL, Proteinase K, and Buffer AL to the mixture. It was incubated for a week, then spun through a centrifuge and debris was removed. We added ethanol. then transfered it to a spin column. We centrifuged it for 1 minute, then added buffers AW1 and AW2. We moved it back to a microcentrifuge tube, then incubated for 1 hour after adding Buffer AE to the sample. We added a primer, then ran it through a Mini one gel electrophoresis machine.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_7248-scaled.jpg,",35.4076868790,-82.5008921000,12/11/2024,"
We found a spider (xysticus)<\/p>\n",Araneae,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_2800-scaled.jpg,","
We started with scooping the guts out for the DNA. We than extracted the DNA using buffers, and put that DNA into the electrophoresis system for 30 minutes.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_7251-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_7252-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_7249-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_7250-scaled.jpg,",35.4076868792,-82.5008921064,12/11/2024,"
We found a stinkbug underneath Tanya Praters table in her classroom.<\/p>\n",Pentatomidae,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_2792-scaled.jpg,","
We first started with collecting our insects. we than extracted the DNA from the abdomen of the stinkbug, than went through the procedure of using our purified Dna prepared PCR samples separately for the arthropod gene and the wolbachia gene. for electrophoresis we used the mini PCR machines and ran our PCR products for 30 minutes each.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Ahpids14Jan2025_2-1.jpg,",40.2927851000,-76.8834460000,01/14/2025,"
Moving around on plant<\/p>\n
Eating plant material<\/p>\n",Myzus persicae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,Other,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Ahpids14Jan2025_1-2.jpg,",40.2927851000,-76.8834460000,01/14/2025,"
Moving around on plant<\/p>\n
Eating plant material<\/p>\n",Myzus persicae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,Other,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Ahpids14Jan2025_1-1.jpg,",40.2926972480,-76.8833433400,01/14/2025,"
moving around on plant.<\/p>\n
eating plant material.<\/p>\n",Myzus persicae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,Other,SYBR Safe,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Ahpids14Jan2025_2.jpg,",40.2927851000,-76.8834460000,01/14/2025,"
Moving around on plant<\/p>\n
Eating plant material<\/p>\n",Myzus persicae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,Other,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/SingleAphid14Jan2025.jpg,",40.2926972480,-76.8833433400,01/14/2025,"
Moving around on plant<\/p>\n
Eating plant material<\/p>\n",Myzus persicae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,Other,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/Ahpids14Jan2025_1.jpg,",40.2926972481,-76.8833433421,01/14/2025,"
Moving around on plant.<\/p>\n
Eating plant material.<\/p>\n",Myzus persicae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,Other,SYBR Safe,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/WhatsApp-Image-2025-02-04-at-10.23.23.jpeg,",32.0035065998,34.7709859603,12/25/2024,"
Caught on the wall<\/p>\n",Diptera,DNeasy (Qiagen),Whole arthropod,MiniOne,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/geli.jpg,","
1. Caught the bug with our hands
\n2. Place it in a Ethanol-filled test tube
\n3. DNA extraction process
\n4. DNA replication using PCR machine
\n5. electrophoresis<\/p>\n",Yes,NNNNNNAANNTNNNNTTTCNNNGCTTGATCCGGAATAGTAGGAACATCATTAAGTATTTTAATTCGAGCTGAATTAGGAC ATCCTGGTGCTTTAATTGGTGATGACCAAATTTATAATGTAATTGTTACTGCTCATGCATTTATTATAATTTTTTTTATA GTTATACCAATTATAATTGGAGGATTTGGAAATTGACTAGTCCCTTTAATATTAGGAGCCCCAGATATAGCCTTTCCTCG AATGAATAATATAAGTTTCTGACTTTTACCACCAGCTTTAACTTTACTACTAGCCAGTAGTATAGTGGAAAACGGAGCTG GAACTGGTTGAACAGTTTACCCTCCTCTATCATCTAATATCGCTCATGGAGGAGCTTCTGTTGATTTAGCTATTTTTTCA CTACATTTAGCAGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACTACTGTAATTAATATACGATCAACAGGAAT TACTTTCGATCGAATACCTTTATTTGTATGATCAGTAGCTATTACAGCTCTATTATTATTATTATCTTTACCTGTATTAG CAGGAGCTATTACAATATTATTAACAGATCGAAATTTAAATACTTCTTTTTTCGACCCTGCTGGAGGAGGAGACCCAATT TTATATCAACACTTATTTTGATTTTTTGGTCACCCTGAAGTTTAA,https://wolbachiaprojectdb.org/wp-content/uploads/2025/03/tube3_co1-6.fasta,, "",32.0029540240,34.7698122131,12/25/2024,"
It was alive found on a wall<\/p>\n",Arthropoda,DNeasy (Qiagen),Whole arthropod,MiniOne,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/WhatsApp.jpeg,","
Caught the spider with our hands
\nPlace it in a Ethanol-filled test tube
\nDNA extraction process
\nDNA replication using PCR machine
\nelectrophoresis<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/נמלה-וולבכיה.jpeg,",32.1454080000,34.9077504000,12/25/2024,"
We found an ant, in school yard.<\/p>\n",Messor ebeninus,DNeasy (Qiagen),Whole arthropod,,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/gel-1-1.jpeg,","
\n
Caught the ant with our hands<\/li>\n
Place it in a Ethanol-filled test tube<\/li>\n
DNA extraction process<\/li>\n
DNA replication using PCR machine<\/li>\n
electrophoresis<\/li>\n<\/ol>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/harak-no-wolbachia-1.jpg,",32.003491779,34.7710243612,12/25/2024,"
Caught on a leaf of a big bush.<\/p><\/blockquote>\n",Oncopeltus fasciatus,DNeasy (Qiagen),Whole arthropod,MiniOne,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/1000098710.jpg,","
\n
Caught the bug with our hands<\/li>\n
Place it in a Ethanol-filled test tube<\/li>\n
DNA extraction process<\/li>\n
DNA replication using PCR machine<\/li>\n
electrophoresis<\/li>\n<\/ol>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_2235-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_2246-scaled.jpg,",42.4836790000,-71.1423155000,09/26/2024,"
Green, small, and round. Tan legs.<\/p>\n",Harpalus rufipes,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_3059-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_3060-scaled.jpg,","
Wolbachia experiment lab 1 to 2 to 3 to 4 followed as stated.<\/p>\n
1% agarose gel was 0.4g of agarose in 40 mL of buffer.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/unnamed-1-scaled.jpg,",42.4836790000,-71.1423155000,09/26/2024,"
The Grub was found under a Rock and was\u00a0 a really white and some what see through<\/p>\n",Phyllophaga,DNeasy (Qiagen) blood and tissue kit,,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-31-at-12.46.47 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-31-at-12.46.53 PM.png,","
wolbachia lab 1 then followed to lab 2 then to lab 3 then to lab 4. the only thing changed was the amout of agrose gel which was change to 40 ML<\/p>\n",No,,,, "",44.8662492000,-68.7906169000,11/07/2024,"
The Japanese Lady Bug was small with a hard outer layer covering its abdomen. I extracted the abdomen of the lady bug for my PCR test.<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_5981.jpg,","
During my lab procedure everything went as planned, there was no contamination and we got the expected results. The only problem that occurred was that we ran out of positive DNA for our 5th well so we didn’t get a positive DNA result.<\/p>\n",No,,,, "",44.8342071582,-68.7727224650,11/07/2025,"
dead, very long legs, body and head seem to be as one.<\/p>\n",Opiliones,DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL,Abdomen,Standard electrophoresis system,TBE,Other,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_4651-scaled.jpeg,",44.8016128000,-68.7712257000,11/07/2024,"",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_5254-scaled.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/20241107_083600-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/20241107_083538-scaled.jpg,",44.8662492000,-68.7906169000,11/07/2024,"",Lepidoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/1000012404-scaled.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_0256-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_0184-scaled.jpeg,",44.8662492000,-68.7906169000,11/07/2025,"
You can see a part of the Wasps gametes from the arthropods abdomen.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"","
I did not end up running the gel electrophoresis. This is because there was an accidental mix of the taskmaster mix with the overall total DNA gathered from the arthropod abdomen. This ended up ruining the results for the gel electrophoresis.<\/p>\n",,,,, "",44.8656860329,-68.7914984395,11/07/2024,"",Coccinellidae,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_8234.jpg,","
My results were in the wells 2-7.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/1000006065-scaled.jpg,",,,11/07/2025,"",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_0376.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6625-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6626-scaled.jpeg,",44.8646708854,-68.7375178401,11/07/2024,"",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-31-at-9.49.10 AM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6629-scaled.jpeg,",44.8019983578,-68.7695226057,11/07/2024,"",Araneae,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_4357-scaled.jpeg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_3522-1.jpg,",,,11/07/2024,"
Black Wasp.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_3736-scaled.jpeg,",44.8053122000,-68.7685691000,08/31/2024,"",Dermaptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_4278-scaled.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/spiders1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/spiders2-scaled.jpg,",44.8662492000,-68.7906169000,10/15/2024,"",Araneae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/0114250955-scaled.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-06-at-10.03.06 AM.png,",44.8662492000,-68.7906169000,11/07/2024,"
Black Fly<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TBE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_8234.jpg,","
The results for the Black Fly are in the second set of wells titled 1-6.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/A8.jpeg,",41.6760600000,-77.0678900000,10/07/2024,"
Location – On a rock, part of the riverbed<\/p>\n
Notes – None<\/p>\n",Lasius neoniger,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/A8.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6730-1-1-scaled.jpg,",42.4835021188,-71.1422491564,09/26/2024,"",Dissosteira carolina,DNeasy (Qiagen) blood and tissue kit,Gonads dissected,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_7020-2.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/02/IMG_7022-2.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_0502-scaled.jpeg,",44.9333081013,-93.1369187270,09/10/2024,"
I found this ant in an Urban environment, in the city of St. Paul coming into resident’s houses. This occurred during September as summer was transitioning into fall. There have been many flying ants coming into my house, but I only collected one. It was around 70 degrees Fahrenheit.<\/p>\n",Camponotus pennsylvanicus,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-11-155854.png,","
Use SeeGreen DNA dye in 1.5% aragose gel in TBE buffer<\/p>\n
MiniPCR Electrophoresis system<\/p>\n
5\u00b5l DNA ladder<\/p>\n
2\u00b5 loading dye + 8\u00b5 DNA in each lane<\/p>\n",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/AC6_ant-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_5851-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_5852-scaled.jpg,",44.9510901582,-93.1046471203,08/23/2024,"
It was caught on a tree. it has brown legs and a white body.<\/p>\n",Araneae,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/unnamed-scaled.jpg,",44.9314816000,-93.1692544000,08/23/2024,"
Lots of legs, Brown, Small<\/p>\n",Arthropoda,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_5338.jpg,",44.94794749981588,-93.0912514144291,,"
it was late summer\/ early fall<\/p>\n
found in the grass next to a tree<\/p>\n",Hymenoptera,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_3341-scaled.jpeg,",,,03/04/2025,"
Very small, brown\/red, color 6 legs one leg is damaged<\/p>\n",Hymenoptera,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_1075-scaled.jpg,",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_4812-scaled.jpeg,",36.1444896000,-86.8011462000,,"",,,,,,,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-08-125656.png,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-08-125716.png,",44.9314816000,-93.1692544000,08/23/2025,"
light and dark brown<\/p>\n
has legs<\/p>\n
reflective pattern down its back<\/p>\n",Araneae,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/wolbach-scaled.jpg,",44.9259166,-93.17279579999999,08/08/2024,"
Large grey-orange thorax, orange legs, head, and abdomen.<\/p>\n",Araneae,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Rolly-Polly.png,",44.971274229,-93.2813637912,08/22/2024,"
found in the dirt outside of school. curled into a ball the second I\u00a0 picked it up.<\/p>\n",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_9504-scaled.jpg,",44.928516549024486,-93.17056562749528,08/22/2024,"
I found this Ant climbing on a tree in my schools yard. I collected it in the afternoon, around 2:00 PM, and it was a sunny day. There were other ants near the area which I collected this one. $\"\"$ <\/p>\n",Hymenoptera,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-07-142318-2.png,",44.9314816000,-93.1692544000,08/22/2024,"
Round, Grey, stubby, little segments on the back.<\/p>\n",Armadillidium vulgare,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_0053-scaled.jpg,",44.9265103390,-93.1725936057,,"
I found this Rolly Polly in the grass near a bush at my High-School St. Paul Acadamy. I found roughly 2 pm in the Afternoon on August 22. It was about 74 degrees outside. There were multiple other Rolly Polly and other bugs around the area I found my Rolly Polly.<\/p>\n",Armadillidae,,,,,,"","",,,,, "",42.5920977000,-72.6033351000,09/14/2024,"
Collected\u00a0 outside after it died<\/p>\n",Formicidae,DNeasy (Qiagen),Abdomen,MiniOne,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/GD-Wolbachia.png,","
Followed Wolbachia Lab Protocol for students<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/20241106_090211-1-scaled.jpg,",42.5934203575,-72.5932735058,09/14/2024,"",Hymenoptera,DNeasy (Qiagen),Rear half,MiniOne,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/JJ-Wolbachia-Results.png,","
Followed Wolbachia Project Lab Protocol<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_0155-scaled.jpg,",44.9264298435,-93.1728042215,08/22/2025,"
Brown, small, oval, legs<\/p>\n",Arthropoda,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_0743-rotated.jpg,",44.9261630074,-93.1754939783,08/22/2024,"
scaley, round, lots of legs,\u00a0 and small.<\/p>\n",Arthropoda,,,,,,"","",,,,, "",44.9259606380,-93.1732330101,03/02/2025,"
$\"\"$ <\/p>\n",Armadillidium,,,,,,"","",,,,, "",44.9537029000,-93.0899578000,08/23/2024,"
Small, dark<\/p>\n
six legs, two antenea<\/p>\n
two joints in each leg<\/p>\n
clear separation of head, abdomen, thorax<\/p>\n
head tapers into two pincers<\/p>\n",Hymenoptera,,,,,,"","",,,,, "",,,08/23/2024,"
Small and grey<\/p>\n",Arthropoda,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Bio-Bugs-scaled-e1736268791378.jpg,",44.9314816000,-93.1692544000,,"
Exoskeleton, six legs, head, thorax, abdomen<\/p>\n",Formicidae,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Image-8-scaled.jpeg,",47.4901117000,8.5036780000,08/24/2024,"
caught in kitchen<\/p>\n",Vespula germanica,Instagene Matrix,Rear half,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Image-1.png,","
The slots were placed at the wrong place, and there wasn’t enough blue tracing dye in the samples.<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/22_Arthropod-CO1F.fasta,, "",45.4762586687,-122.7580061590,11/19/2024,"
Crawling, clinging to reeds, rapid movement, folded in half when collected.<\/span> Large shield-backed, yellow-blackish colored.<\/span> Many were in same area, all behaved very similarly.\u00a0<\/span><\/p>\n",Hesperocorixa laevigata,,,,,,"","",,,,, "",45.4760262659,-122.7581292374,11/11/2024,"
Collected in wetlands, on edge of pond. Clinging to foliage, fast-moving. Contains head, segmented thorax, abdomen, 8 legs, antennae, but no wings.<\/p>\n",Odonata,,,,,,"","",,,,, "",45.4395124,-122.7711943,11/20/2024,"
Observations and Comments: crysalises were a shade between beige and white colored, and they had small holes in them all over the outer covering, they kind of looked like a seashell with ridged edges<\/b><\/p>\n",Arthropoda,,,,,,"","",Unknown,,,, "",45.474009,-122.75507,11/20/2024,"
Fly was very small, much smaller than the average house fly. It flew around very fast and was quite hard to catch. <\/b><\/p>\n",Arthropoda,,,,,,"","",Unknown,,,, "",45.4775021520,-122.7587600102,11/20/2024,"
\n
Location Description: <\/b>The arthropod was found under tree bark in the wetlands at Oregon Episcopal School.<\/span><\/li>\n
Season: <\/b>Autumn<\/span><\/li>\n
Temperature: <\/b>51\u00ba<\/span><\/li>\n
Habitat:<\/b> Terrestrial<\/span><\/li>\n
Behavioral Observations: <\/b>Upon uncovering, the arthropod quickly moved toward surrounding tree bark.\u00a0<\/span><\/li>\n
Physical Description:<\/b>\n
\n
Number of legs: 28<\/span><\/li>\n
Presence of wings: No<\/span><\/li>\n
Presence of antennae: Yes<\/span><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n",Chilopoda,,,,,,"","",,,,, "",45.4963538000,-122.7216995000,11/17/2024,"
<\/p>\n
\n
Location Description:<\/b> The arthropod was found on the surface of a domesticated dog.<\/span><\/li>\n
Season: <\/b>Autumn<\/span><\/li>\n
Temperature: <\/b>71\u00ba<\/span><\/li>\n
Habitat: <\/b>Other<\/span><\/li>\n
Behavioral Observations: <\/b>Moved quickly and attempted to flee.<\/span><\/li>\n
Body segmentation (head, thorax, abdomen)<\/span><\/li>\n
Number of legs: 6<\/span><\/li>\n
Presence of wings: no<\/span><\/li>\n
Presence of antennae: yes<\/span><\/li>\n<\/ul>\n",Ctenocephalides felis,,,,,,"","",,,,, "",45.4740108660,-122.7545311973,11/20/2024,"
Very small, crawled very quickly around the tube and collection area, hard to place in collection tube<\/p>\n",Arachnida,,,,,,"","",,,,, "",45.4737847565,-122.7548192176,11/20/2024,"
We found this bug on the curtain of the school nurse’s office between 8:30 and 9:30 a.m. It was very cold outside but the bug was inside at around room temperature, so maybe around 75 degrees Fahrenheit.<\/p>\n",Leptoglossus occidentalis,,,,,,"","",,,,, "",45.4742068007,-122.7568815983,11/20/2024,"
I found it below the dead log around 11:30 am. The temperature was about 10.5 C. The log was placed on many wet leaves.<\/p>\n",Armadillidium vulgare,,,,,,"","",,,,, "",45.47411,-122.75458,11/20/2024,"
\n
Body segmentation: Head, thorax, abdomen<\/span><\/li>\n
Number of legs: 6<\/span><\/li>\n
Presence of wings: Yes<\/span><\/li>\n
Presence of antennae: Yes<\/span><\/li>\n<\/ul>\n",Diptera,,,,,,"","",,,,, "",45.4772800000,-122.7582200000,11/20/2024,"
On the shore by the OES Wetlands on some leaves.<\/p>\n
51\u00ba F<\/p>\n
\n
Body segmentation (e.g., head, thorax, abdomen): head<\/span><\/li>\n
Number of legs: 6<\/span><\/li>\n
Presence of wings: Yes<\/span><\/li>\n
Presence of antennae: Yes<\/span><\/li>\n<\/ul>\n
<\/p>\n",Diptera,,,,,,"","",,,,, "",45.4739332879,-122.7542453896,11/20/2024,"
This Bark Centipede was collected next to a greenhouse in the soil beneath some concrete blocks. The soil was moist and surrounded by grass and overgrown plants. The specimen was collected at around noon with a temperature of 10.6 \u2103 at the tail end of fall. Arthropods were scarce at this time of day\/year and only this centipede was beneath the chosen stone.<\/span><\/p>\n",Cryptopidae,DNeasy (Qiagen),Abdomen,,,,"","",,,,, "",45.4739332870,-122.7542453000,11/20/2024,"
This Bark Centipede was collected next to a greenhouse in the soil beneath some concrete blocks. The soil was moist and surrounded by grass and overgrown plants. The specimen was collected at around noon with a temperature of 10.6 \u2103 at the tail end of fall. Arthropods were scarce at this time of day\/year and only this centipede was beneath the chosen stone.<\/span><\/p>\n",Cryptopidae,DNeasy (Qiagen),Abdomen,,,,"","",,,,, "",45.4740577,-122.7553359,11/20/2024,"
Behavioral Observations:<\/b><\/p>\n
Very fast with rapid movements. It almost avoided the light when the tree bark was pulled back.<\/p>\n
Location:\u00a0<\/strong><\/p>\n
Found in Oregon Episcopal School wetlands under the bark of a tree branch.<\/p>\n
Physical Description:<\/b><\/p>\n
\n
\n
\n
Body segmentation (e.g., head, thorax, abdomen)<\/span><\/li>\n
Number of legs: 34<\/span><\/li>\n
Presence of wings: No<\/span><\/li>\n
Presence of antennae: Yes<\/span><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n",,,,,,,"","",,,,, "",45.4740483000,-122.7553890000,11/20/2024,"
Observations:<\/b><\/p>\n
Behavioral Observations:<\/strong><\/p>\n
\n
Scurried across the branch when the bark of the tree was pulled back<\/li>\n<\/ul>\n
Location:<\/strong><\/p>\n
\n
Found in the Oregon Episcopal School wetlands under the bark of a tree branch (51\u00ba F)<\/li>\n<\/ul>\n
Physical Description:<\/b><\/p>\n
\n
Body segmentation (e.g., head, thorax, abdomen) 12 different body segments from head to tail<\/span><\/li>\n
Number of legs: 14<\/span><\/li>\n
Presence of wings: No<\/span><\/li>\n
Presence of antennae: Yes<\/span><\/li>\n
<\/li>\n<\/ul>\n",Armadillidae,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/3446AE1B-8149-4433-B5C8-4EC7927DFDE7.jpeg,",41.7756400000,-88.0970000000,09/10/2024,"
\n
collected by Fall 2024 students<\/li>\n<\/ul>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","
\n
\n<\/blockquote>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bug-11.png,",37.9703726261,-84.5043897515,09/16/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol.<\/p>\n
The specimen was identified as a Picture-winged Fly Delphinia using the iNaturalist app.<\/p>\n",Tephritidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bug-11-gel.png,","
GoTaqGreen<\/span> polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 <\/span>uL<\/span>. 5 <\/span>uL<\/span> of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes for the specimen are boxed in the gel picture.\u202f\u202f<\/span><\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bug-10.png,",37.9744322832,-84.5043897515,09/10/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol.<\/p>\n
The specimen was identified as a Mosquito using the iNaturalist app.<\/p>\n",Culicidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Mosquito-10-gel.png,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 uL. 5 uL of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes for the specimen are boxed in the gel picture.<\/p>\n",Yes,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Mosquito-1.png,",37.9725378045,-84.5030164604,09/08/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol.<\/p>\n
The specimen was identified as an Asian Tiger Mosquito using the iNaturalist app.<\/p>\n",Aedes,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Gel-mosquito-1.png,","
GoTaqGreen<\/span> polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 <\/span>uL<\/span>. 5 <\/span>uL<\/span> of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes for the specimen are boxed in the gel picture.\u202f\u202f<\/span><\/span>\u00a0<\/span><\/p>\n",Yes,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bug-18.png,",38.2097967000,-84.5588311000,,"
Caught in backyard of residence. The specimen was identified as a stink bug using the iNaturalist app.<\/p>\n",Pentatomidae,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Wolbachia-Project-PCR-results-bls-bug-18.png,","
GoTaqGreen<\/span> polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 <\/span>uL<\/span>. 5 <\/span>uL<\/span> of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes for the specimen are boxed in the gel picture.\u202f\u202f<\/span><\/span>\u00a0<\/span><\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bug-19.png,",38.2097967000,-84.5588311000,,"
Caught in backyard of residence. The specimen was identified as a stink bug using the iNaturalist app.<\/p>\n",Pentatomidae,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Wolbachia-Project-PCR-results-bls-bug-19-1.png,","
GoTaqGreen<\/span> polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 <\/span>uL<\/span>. 5 <\/span>uL<\/span> of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes for the specimen are boxed in the gel picture.\u202f\u202f<\/span><\/span>\u00a0<\/span><\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Mosquito-14.png,",37.9734321727,-84.5037561380,09/08/2024,"
The specimen was collected using a flying insect trap <\/span>containing<\/span> banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch<\/span> adjacent to<\/span> a small creek in a suburban backyard<\/span> for two days<\/span>.<\/span> The trap was cut open and <\/span>the (<\/span>predominantly <\/span>flying<\/span>)<\/span> insects were removed and preserved in 70% alcohol. <\/span><\/p>\n
The specimen was identified as a Mosquito using the iNaturalist app.<\/p>\n",Culicidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Gel-for-14.png,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 uL. 5 uL of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes are boxed in the gel picture.<\/p>\n",Yes,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Moth-16.png,",37.9722671607,-84.5019864922,09/09/2024,"
The specimen was collected using a flying insect trap <\/span>containing<\/span> banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch<\/span> adjacent to<\/span> a small creek in a suburban backyard<\/span> for two days<\/span>.<\/span> The trap was cut open and <\/span>the (<\/span>predominantly <\/span>flying<\/span>)<\/span> insects were removed and preserved in 70% alcohol.<\/span><\/p>\n
T<\/span>he specimen was <\/span>identified as a Moth – Lepidoptera <\/span><\/span>using the <\/span>iNaturalist<\/span> app.\u202f<\/span><\/span>\u00a0<\/span><\/p>\n",Lepidoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Gel-for-16.png,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 uL. 5 uL of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes for the specimen are boxed in the gel picture.<\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bee-15.png,",37.9725378045,-84.5023298149,09/10/2024,"
The specimen was collected using a flying insect trap <\/span>containing<\/span> banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch<\/span> adjacent to<\/span> a small creek in a suburban backyard<\/span> for two days<\/span>.<\/span> The trap was cut open and <\/span>the (<\/span>predominantly <\/span>flying<\/span>)<\/span> insects were removed and preserved in 70% alcohol.<\/span><\/p>\n
The specimen was identified as a Bee – Apoidea using the iNaturalist app.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Gel-for-15.png,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 uL. 5 uL of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes are boxed.<\/p>\n",Yes,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Drosophila-Fruit-Fly-13.png,",37.9709139267,-84.5030164604,09/08/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol. The trap was then placed on the ground for an additional three days capturing additional insects. These insects included both crawling and flying insects. These insects were removed and preserved in 70% alcohol.<\/p>\n
The specimen was identified as a Drosophila Fruit Fly using the iNaturalist app.<\/p>\n",Drosophila,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Gel-for-13.png,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 uL. 5 uL of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes for the specimen #13 are boxed in the gel picture.<\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/bug-17-.png,",38.1813561096,-84.6152681355,10/27/2024,"
Was collected at a window in my house using toilet paper to capture them without crushing them, then putting them in a Ziplock bag full isopropyl alcohol to preserve until DNA extraction proceeded.<\/p>\n
The insect was identified as Genus Halyomorpha using the iNaturalist app.<\/p>\n",Halyomorpha,Qiagen Blood and Tissue kit,Whole arthropod,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Wolbachia-Project-PCR-results-bls-bug-17-1.png,","
GoTaqGreen<\/span> polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). <\/span>The PCR reaction volume was 25 <\/span>uL<\/span>. 5 <\/span>uL<\/span> of each reaction was run on a 2% agarose gel.\u202f<\/span>The relevant lanes, lanes 2<\/span><\/span> and 3<\/span><\/span>, for the specimen (#17<\/span><\/span>) are boxed.\u202f\u202f<\/span><\/span><\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Bug-24-ed2.jpg,",37.9706432769,-84.5006132012,09/09/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard.<\/p>\n
The specimen was identified as a Stripe-horned Green Lacewing using the iNaturalist app.<\/p>\n",Chrysopidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Gel-Number-24-1.jpg,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 uL. 5 uL of each reaction was run on a 2% agarose gel. The relevant lanes, lanes 8 and 9, for the specimen (#24) are boxed.<\/p>\n",No,Sequencing not performed,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Bug-26-ed.jpg,",37.9716353487,-84.5010806309,09/10/2024,"
The specimen was discovered feeding on my leg. It was gently swatted and preserved in 70% alcohol until analysis.<\/p>\n
The specimen was identified as an Aedes mosquito<\/span><\/span> using the iNaturalist app.<\/p>\n",Aedes,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Gel-Number-26.jpg,","
GoTaqGreen polymerase was used for the PCR. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 uL. 5 uL of each reaction was run on a 2% agarose gel. The relevant lanes, lanes 4 and 5, for the specimen (#26) are boxed.<\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/mosquito-27-2.png,",37.9717258701,-84.5023298149,09/08/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard.<\/p>\n
The specimen was identified as an Aedes using the iNaturalist app.<\/p>\n",Aedes,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bug-27-gel.png,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for duplex reaction PCRs. The PCR reaction volume was 25 uL. 10 uL of each reaction was run on a 2% agarose gel. The relevant gel lane (lane 2) for the specimen #27 is boxed.<\/p>\n",Yes,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bee-28.png,",37.9709139267,-84.5030164604,09/16/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol. The trap was then placed on the ground for an additional three days capturing additional insects. These insects included both crawling and flying insects. These insects were removed and preserved in 70% alcohol.<\/span>\u00a0<\/span><\/p>\n
The specimen was identified as a <\/span>bee (Anthophila)<\/span>\u00a0using the iNaturalist app.\u202f<\/span>\u00a0<\/span><\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bug-28-gel.png,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for duplex reaction PCRs. The PCR reaction volume was 25 uL. 10 uL of each reaction was run on a 2% agarose gel. The relevant lane (lane 3), for the specimen #28 is boxed.<\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Earwig-29.png,",37.9725378045,-84.5040464287,09/16/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol. The trap was then placed on the ground for an additional three days capturing additional insects. These insects included both crawling and flying insects. These insects were removed and preserved in 70% alcohol.<\/p>\n
The specimen was identified as an earwig (Forficulinae) using the iNaturalist app.<\/p>\n",Forficulidae,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bug-29-gel.png,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for duplex reaction PCRs. The PCR reaction volume was 25 uL. 10 uL of each reaction was run on a 2% agarose gel. The relevant lane \u00a0(lane 4) for the specimen #29 is boxed.<\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Fruit-fly-30.png,",37.9719965159,-84.5037031060,09/08/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol. The trap was then placed on the ground for an additional three days capturing additional insects. These insects included both crawling and flying insects. These insects were removed and preserved in 70% alcohol.<\/p>\n
The specimen was identified as a fruit fly (Drosophila) using the iNaturalist app.<\/p>\n",Drosophila,Blood and Tissue kit,Whole arthropod,Standard electrophoresis system,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Bug-30-gel.png,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for duplex reaction PCRs. The PCR reaction volume was 25 uL. 10 uL of each reaction was run on a 2% agarose gel. The relevant lane (lane 5) for the specimen #30 is boxed.<\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Slide3.jpg,",37.9690193572,-84.4872236138,09/09/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol. The trap was then placed on the ground for an additional three days capturing additional insects. These insects included both crawling and flying insects. These insects were removed and preserved in 70% alcohol.<\/span>\u00a0<\/span><\/p>\n
The specimen was identified as a Culicine Mosquito<\/span>\u00a0using the iNaturalist app.<\/span><\/p>\n",Aedes albopictus,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Sample-7-Culicine-Asian-Tiger-Mosquito.png,","
GoTaqGreen<\/span> polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). <\/span>The PCR reaction volume was 25 <\/span>uL<\/span>. 5 <\/span>uL<\/span> of each reaction was run on a 2% agarose gel.\u202f<\/span>The relevant lanes, lanes 7\u00a0<\/span><\/span> and 8<\/span><\/span>, for the specimen (#8<\/span><\/span>) are boxed.\u202f\u202f<\/span><\/span>\u00a0<\/span><\/p>\n",Yes,The PCR product was not sequenced,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Slide1.jpg,",37.9684780427,-84.4865369683,09/09/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol. The trap was then placed on the ground for an additional three days capturing additional insects. These insects included both crawling and flying insects. These insects were removed and preserved in 70% alcohol.<\/span>\u00a0<\/span><\/p>\n
The specimen was identified as a Musca Face Fly<\/span>\u00a0using the iNaturalist app.<\/span><\/p>\n",Musca,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Sample-5-Musca.png,","
GoTaqGreen<\/span> polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). <\/span>The PCR reaction volume was 25 <\/span>uL<\/span>. 5 <\/span>uL<\/span> of each reaction was run on a 2% agarose gel.\u202f<\/span>The relevant lanes, lanes 5 <\/span><\/span>and 6<\/span><\/span>, for the specimen (#5<\/span><\/span>) are boxed.\u202f\u202f<\/span><\/span>\u00a0<\/span><\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Slide2.jpg,",37.9695606678,-84.4855070000,09/09/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol. The trap was then placed on the ground for an additional three days capturing additional insects. These insects included both crawling and flying insects. These insects were removed and preserved in 70% alcohol.<\/span>\u00a0<\/span><\/p>\n
The specimen was identified as a Diptera<\/span>\u00a0using the iNaturalist app.<\/span><\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Sample-6-Diptera.png,","
GoTaqGreen<\/span> polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). <\/span>The PCR reaction volume was 25 <\/span>uL<\/span>. 5 <\/span>uL<\/span> of each reaction was run on a 2% agarose gel.\u202f<\/span>The relevant lanes, lanes 3 <\/span><\/span>and 4<\/span><\/span>, for the specimen (#6<\/span><\/span>) are boxed<\/span><\/span><\/p>\n",No,The PCR product was not sequenced,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Slide4-2.jpg,",37.9692900130,-84.4855070000,09/09/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol. The trap was then placed on the ground for an additional three days capturing additional insects. These insects included both crawling and flying insects. These insects were removed and preserved in 70% alcohol.<\/span>\u00a0<\/span><\/p>\n
The specimen was identified as a Diptera<\/span>\u00a0using the iNaturalist app<\/span><\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Sample-8-Diptera.png,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 uL. 5 uL of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes, lanes 1 <\/span>and 2<\/span>, for the specimen (#8<\/span>) are boxed.\u202f\u202f<\/span>\u00a0<\/span><\/p>\n
\u00a0<\/span><\/p>\n",No,The PCR product was not sequenced,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_7600-1-scaled.jpeg,",41.8011410000,-88.0747875000,09/03/2024,"
Collected by Fall 2024 students<\/p>\n",Magicicada septendecim,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_0143-scaled.jpeg,","
<\/p><\/blockquote>\n",Yes,,,, "",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_0125-scaled.jpg,",41.7767986245,-88.0980583454,09/04/2024,"
Collected by Fall 2024 students. The moth was caught using a sweep net and a killing jar, on grass near bushes.<\/p>\n",Autographa precationis,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/gel-2.png,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Autographa.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Ground_cricket_neonemobius.jpg,",38.0624674975,-84.4975454035,09/10/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol. The trap was then placed on the ground for an additional three days capturing additional insects. These insects included both crawling and flying insects. These insects were removed and preserved in 70% alcohol.<\/p>\n
The specimen was identified as a Small Ground Crickets Genus Neonemobius using the iNaturalist app.<\/p>\n",Orthoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-13-124023.png,","
GoTaqGreen<\/span> polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). <\/span>The PCR reaction volume was 25 <\/span>uL<\/span>. 5 <\/span>uL<\/span> of each reaction was run on a 2% agarose gel.\u202f<\/span>The relevant lanes <\/span><\/span>are boxed.\u202f\u202f<\/span><\/span>\u00a0<\/span><\/p>\n",Yes,The PCR product was not sequenced,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Anthropila_Bee_Photo.jpg,",38.0615176766,-84.4983146390,10/21/2024,"
The specimen was collected using a flying insect trap containing banana peels and vinegar per the Arthropod Collection Traps Guide provided by the Wolbachia Project. The trap was hung from a tree branch adjacent to a small creek in a suburban backyard for two days. The trap was cut open and the (predominantly flying) insects were removed and preserved in 70% alcohol. The trap was then placed on the ground for an additional three days capturing additional insects. These insects included both crawling and flying insects. These insects were removed and preserved in 70% alcohol.<\/p>\n
The specimen was identified as an Anthrophila bee using the iNaturalist app.<\/p>\n",Anthophila,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system Bio-Rad,TAE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Gel_Anthophila_Bee.jpg,","
GoTaqGreen polymerase was used. Reaction conditions were per Lab 3: PCR (Wolbachia Project Guide) for single reaction PCRs (separate reactions were run with the Wolbachia primers and the cytochrome oxidase primers). The PCR reaction volume was 25 uL. 5 uL of each reaction was run on a 2% agarose gel.\u202fThe relevant lanes are boxed.<\/p>\n",No,The PCR product was not sequenced.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/668853F0-B37A-4452-A9E2-C6B78AD6691A_1_105_c.jpeg,",41.77564,-88.0970,09/04/2024,"
It was caught in the grass with a killing jar.<\/p>\n",Vespula maculifrons,DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/F0F7A839-9918-42CA-B639-B98B96F8AF0A_1_105_c.jpeg,","",No,>74-C_AG1_Co1R.scf (612 bp) CGAAAAAAGATGTGTTTAAGTTCCGATCTGTTAAAAGTATAGTAATNGCTCCTGCAAGAACTNGTAGTGATAATAGAAGA AGAATTGCTGTAATTAAAATTGATCATGTGAATAAAGGAAGAAAATTTAATGAATGTGTTTTTGTGTGTATATTTAAAAT AGTAACGATAAAATTAATTGATCCTATAATTGATGAAATTCCAGCAATATGAATTGAAAAAATTCCTAAATCTACAGATG GAGAATCATGACCAACAATAGAAGATAAAGGAGGGTATAAAGTTCATCCTGTTCCTACCCCTGTTCCAATAAAATTTCTT AAAATTAGTAAAAATAATGAAGGAGGGAGTAATCAAAATCTTATATTATTTATTCGAGGAAATGCTATATCAGGTACACC TAGTATTAAAGGGATTAATCAGTTTCCAAATCCTCCTACTAAAAAAGGTATAACTATAAAGAAAATTATAATAAAGGCAT GAGCTGTAATAATTGNATTATAAATTTGATCNTTATTAATTAANNNTNCNGGANANCTTAATTCTAAGCNAANAANTATT CTNATNGAAGCTCCTAAAGTCCCTGCCCATNAAGCAAAAANAAAATANAATN,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/74-C_AG1_Co1R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_4814-scaled.jpeg,",41.7762930909,-88.0969142730,09/04/2024,"
Collected by me was collected using a sweeping net on the concrete sidewalk.<\/p>\n",Melanoplus femurrubrum,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_5225-scaled.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_8567.png,",41.7756400000,-88.0970000000,09/03/2024,"
collected by fall ’24 students<\/p>\n
collected using sweep net near bushes<\/p>\n
<\/p>\n",Erythemis simplicicollis,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_8558-scaled.jpg,","",No,CTGCTCACGCATTCGTTATAATTTTCTTCATAGTTATGCCTATTATAATTGGTGGCTTTGGAAACTGGTTAGTTCCTTTA ATATTAGGAGCCCCGGATATAGCCTTCCCTCGTCTTAATAATATAAGTTTTTGATTACTTCCTCCCTCCTTCACATTACT TTTATCTAGAAGTATAGTAGAAAGAGGAGCAGGAACAGGATGAACTGTATATCCTCCATTGGCAGGAGCTATTGCACATG CAGGAGCATCTGTAGATTTAACTATTTTCTCGTTACACTTAGCAGGAGTATCCTCCATTCTAGGAGCTATTAATTTTATT ACAACAGTAATCAATATAAAATCCCCTGGGATGAAGCTGGATCAAATACCTCTTTTTGTATGAGCAGTAGTAATTACTGC AGTATTATTACTTTTATCCCTTCCTGTATTAGCAGGAGCTATTACTATACTTTTAACAGATCGTAATATCAACACTTCAT TCTTCGACCCAGCAGG,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/57-A_AV4_Co1R.zoha_.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_4378.png,",41.77631973419371,-88.09728964488977,09/04/2024,"",Orthoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/tempImageYs6Kao.jpg,","
$\"\"$ <\/a><\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/dna.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Mosquito-scaled.jpg,",44.8149747000,-93.2080991000,09/16/2024,"
Arthropod has three body segments (head, thorax, and abdomen) with six legs, 2 antennae, and a proboscis. It has a striped abdomen with its limbs having joints, two eyes with a slightly furry thorax, and legs. It was collected in the Fall in a house where the temperature was room temperature but it came from outside which is its normal physical habitat<\/p>\n",Culicidae,DNeasy (Qiagen) blood and tissue kit,body without wings,MiniPCR,TBE,SeeGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Gel-Electrophoresis-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Gel-Electrophoresis.jpg,","
First Gel Electrophoresis didn’t show results (My Arthropod DNA in Lane 6). The gel may have been punctured in the second trial in Lane 4 (My Arthropod DNA in Lane 8).<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Arthropod-Picture-scaled.jpg,",41.7756400000,-88.0970000000,09/03/2024,"
Collected by Fall 2024 student<\/p>\n",Formica incerta,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Gel-Picture-2-.png,","",Yes,TTAAGTTTCGATCAGTTAATAATATTGTAATAGCTCNAGCTAAAACGGGAAGGGATAATAAAAGTAAAATTGCAGTGATT AAGATAGATCATACAAGTAGGGGAATTTTATCAATAGAAAAATTTTTATGATGTATATTANNGATTGTTGAAATAAAATT AATAGCTCCTAAAATAGATGATATACCGGCAATATGAAGAGAAAAAATTGTTAAGTCTACAGAAGGCCCATTATGAAAAA TATTTGAAGCTAAAGGANGGTANATAGTTCACCCAGTTCCAGTACCATCATTAATAAAGTTTCTTAAAAGTAATAGAGTG ANGNANGGAGGTAAAAGTCAAAATCTTATATTATTTATACGAGGATAAGCTATATCTGGNGATCCTAATATTAAAGGAAT TAGAAAATTTCCNAATCCACCAATTATAAATGGTATTACTATAAAAAAGA,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/42-A_AP2_Co1R-Paul-Arthropod.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_0916-rotated.jpeg,",41.77564,-88.0970,09/04/2024,"
$\"\"$ Collected by Fall 2024 students<\/p>\n
Sweep net while on a bush<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_1088-scaled.jpeg,","
$\"\"$ $\"\"$ $\"\"$ <\/p>\n",Yes,N/A,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/1778F7FE-1-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/038005DC-1-1.jpg,",41.77564,-88.0970,09/04/2024,"
Collected by Fall 2024 students.<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_0092-2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_9056.jpg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/53-COForward-fasta.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_8467.png,",41.7756400000,-88.0970000000,09/04/2024,"
collected by Fall 2024 students<\/p>\n",Lepidoptera,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_0092-1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_2532-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_2531-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_3033-scaled.jpg,",44.9496420000,-93.0931240000,09/16/2024,"
It crawled fast on the ground and tried to use the grass as cover.<\/p>\n",Formica glacialis,DNeasy (Qiagen) blood and tissue kit,Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_9308-2-scaled.jpg,","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/EB5_ant-CO1F-2.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_7004-rotated.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_6976.png,",41.7756841800,-88.0964806418,09/04/2024,"
Collected by Fall 2024 students<\/p>\n",Conocephalus brevipennis,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_7111-rotated.jpg,","",No,TATTTAAATTTCGATCAGTTAATAATATAGTAATAGCTCCAGCTAATACTGGTAAAGATAATAATAAAAGTAGTGCTGTA ATAGCTACGGCCCATACAAATAAGGGAGTTTGATCCAGGGATATTCCTGGAGCTCGTATATTAATTGTTGTAGTAATAAA ATTTACAGCTCCTAAAATAGAGGAAATACCTGCTAAATGTAATGAGAAGATCGCTAAATCGACTGAGGCTCCTGCATGGG CAATATTAGCAGAAAGTGGAGGGTAAACTGTTCAACCAGTGCCTGCCCCGTTTTCGACAAGGCTTCTGGCTAGAAGCAAG GTCAAAGATGGGGGTAAAAGTCAAAAACTCATATTGTTTATTCGAGGGAAAGCTATGTCTGGGGCTCCCAGTATTAAAGG AACTAATCAATTCCCGAATCCTCCGATTATAATTGGTATAACTATAAAAAAAATTATAACAAAGGCATGTGCTGTTACAA TAACGTTATAA,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/copy-71-C_AY2_Co1R-1.fa,, "",44.9339600000,-93.1619600000,09/19/2024,"
Brown body, light brown legs<\/p>\n",Lasius neoniger,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,GelGreen,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_1950-1-scaled.jpg,",19.4889833000,-155.9101252000,11/14/2024,"
(Leafhopper?) was in my backyard in the morning around 7:40 when I collected it.<\/p>\n",Hemiptera,Edwards Buffer,Whole arthropod,Edvotek Gel Electrophoresis,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_2023.jpg,","
Gel lanes (Top)<\/p>\n
\n
DNA ladder<\/li>\n
Sample 1 CO1<\/li>\n
Sample 1 16S<\/li>\n<\/ol>\n
<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/1000033097-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/1000033100-scaled.jpg,",36.0797220000,-86.7701886000,10/19/2024,"
Found in grassy yet shaded rolling hills with a hollow and cavernous concrete box and peach trees within 3-5 meters of collection<\/p>\n",Reduvius personatus,pcr,Abdomen,,,,"","",,,,, "",43.5917906000,-70.3364593000,10/18/2024,"
It is a small black ant.<\/p>\n",Insecta,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Untitled-drawing-3.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Untitled-drawing-1-3.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_2024-1-scaled.jpg,",43.5915313157,-70.3356052436,,"",Annelida,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,rotisafe gel stain,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_2917-scaled.jpeg,",41.7756400000,-88.0970000000,09/03/2024,"
collected by Fall 2024 students with a sweep net in the bushes<\/p>\n",Dolichovespula maculata,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/55-A_AV1_Co1RNAFEESAFLIP.fa,, "",36.08036740876806,-86.77080514965017,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/PHOTO-2024-09-10-18-21-03.jpg,",41.7784418000,-88.0952683000,09/03/2024,"
Collected by Fall 2024 students using an aspirator between the sidewalk by the staff lot near the pond by the staff lot.<\/p>\n",Formica pallidefulva,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/PHOTO-2024-10-16-07-23-41.jpg,","",Yes,Formica pallidefulva voucher FKH-010 cytochrome c oxidase subunit I (COX1) gene, partial cds, mitochondrial,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/A-albopictus.jpeg,",39.830921,-77.238802,09/21/2024,"
Rural area, near a wetland<\/p>\n",Aedes albopictus,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TBE,GelGreen,"","
Lane 12, next to positive control<\/p>\n",Yes,TTCGGTATTTGATCTGGAATAGTCGGAACTTCACTAAGAGTTTTAATTCGTATTGAACTTAGACATCCTGGTATATTTATTGGAAATGATCAAATTTATAATGTAATTGTTACTGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCTATCATAATTGGAGGATTTGGAAACTGACTAGTACCCTTAATACTAGGAGCCCCTGATATAGCTTTTCCTCGAATAAATAATATAAGTTTTTGAATATTACCCCCCTCTTTAACACTGCTGCTTTCTAGTTCTATAGTAGAAAACGGAGCTGGAACAGGGTGAACGGTTTATCCTCCTCTTTCTTCTGGAACAGCTCATGCTGGGGCTTCAGTTGATTTAGCAATTTTTTCTTTACATTTAGCGGGAATCTCATCTATTTTAGGAGCAGTAAATTTTATTACAACTGTAATTAATATACGATCAGCTGGTATTACTCTTGATCGACTACCTTTATTTGTGTGATCAGTAGTAATTACAGCTATTTTATTACTTCTTTCTCTACCCGTATTAGCCGGAGCTATTACTATATTATTAACAGACCGAAATTTAAATACATCTTTTTTTGATCCAATTGGAGGGGGAGACCCTATTTTATATCAACATTTATTTTGATTTTTNG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Emily-O-trivitattus.jpeg,",39.8625490000,-77.2751120000,09/19/2024,"
Rural area near wetland<\/p>\n",Culicidae,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-08-at-8.14.09 AM-5.png,","
Lane 11<\/p>\n",No,AAGATATTGGAACATTATACTTCATCTTTGGAGTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGATTAGTTCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATGGTAGAAAATGGATCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGAGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTTTTATCTCTTCCTGTATTAGCCGGAGCTATTACAATATTGTTAACTGACCGAAATCTAAATACATCCTTCTTTGACCCAATTGGTGGGGGAGACCCAATTCTATACCAACATTTATTCTGATTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Jess-Kristen-Aedes-albopictus.jpeg,",39.8309210000,-77.2388020000,09/23/2024,"
Suburban neighborhood<\/p>\n",Aedes albopictus,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-08-at-8.14.09 AM-5.png,","
Lane 10<\/p>\n",Yes,ANTAGGGTCTCCCCCTCCAATTGGATCAAAAAAAGATGTATTTAAATTTCGGTCTTTGATCTAATATAGTCGGAACTTCGCTTAATACGGGTAGAGAAAGAAGTAATAAAAGAGTTTTAATTCGTATTGAACTTAGACATCAAGGTAGTCGATCAAGAGTAATACCAGCTGATCGTATATTAATTGGAAATGTAATAAAATTTATTGCTCCTAAAATAGATGAGATTCCCGCTAAATGTAAAGAAAAAATTGCTAAATCAACTGAAGCCCCAGCATGAGCTGTTCCAGAAGAAAGAGGAGGATAAACCGTTCACCTTGTTACTGCTCATGCTTTTATTATAGAACTAGAAAGCAGCAGTGTTAAAGAGGGGGGTAATATTCAAAAACTTATTTTTTTTATTCGAGGAAAAGCTATATCAGGGGCTCCTAGTAATAAGGGTACTAGTCAGTTTCCAAATCCTCCAATTATGATAGGTATTACTATAAAAAAAATCATAATTGGAGCATGAGCAGTAACAATTACATTGGAAATCTGACTAGTACCCTTAAATATACTAGGATGTCTAAGTTCAATACGAATTAAAGCCCCTGATATAGTTCCGACTTTTCCAGATCGAATACCGAAAATAATATATAGTTTTTGAGTATTAGATCTGGAATAGTCGGAACTTCACTAAGAGTTTTAATTCGTATTGAACTTAGACATCCTGGTATATTTATTGGAAATGATCAAATTTATAATGTAATTGTTACTGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCTATCATAATTGGAGGATTTGGAAACTGACTAGTACCCTTAATACTAGGAGCCCCTGATATAGCTTTTCCTCGAATAAATAATATAAGTTTTTGAATATTACCCCCCTCTTTAACACTGCTGCTTTCTAGTTCTATAGTAGAAAACGGAGCTGGAACAGGGTGAACGGTTTATCCTCCTCTTTCTTCTGGAACAGCTCATGCTGGGGCTTCAGTTGATTTAGCAATTTTTTCTTTACATTTAGCGGGAATCTCATCTATTTTAGGAGCAGTAAATTTTATTACAACTGTAATTAATATACGATCAGCTGGTATTACTCTTGATCGACTACCTTTATTTGTGTGATCAGTAGTAATTACAGCTATTTTATTACTTCTTTCTCTACCCGTATTAGCCGGAGCTATTACTATATTATTAACAGACCGAAATTTAAATACATCTTTTTTTGATCCAATTGGAGGGGGAGACCCTATTTTATATCAACATTTATTTTGATTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Stefan-and-Mike-albopictus.jpeg,",39.8309210000,-77.2388020000,09/21/2024,"
Suburban neighborhood<\/p>\n",Aedes albopictus,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-08-at-8.14.09 AM-5.png,","
Lane 9<\/p>\n",Yes,AGATATTGGAACATTATATTTTATTTTTGGGGCTTGAGCTGGAATAGTTGGAACTTCTTTAAGTTTACTAATTCGAGCAGAATTAAGTCAACCAGGTGTATTTATTGGAAATGATCAAATTTATAATGTTATTGTAACTGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCAATCATAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATGTTAGGAGCTCCAGATATGGCCTTTCCTCGAATAAATAATATAAGTTTTTGAATACTACCTCCTTCATTGACACTACTACTTTCAAGTAGTTTAGTAGAAAATGGAGCTGGGACTGGATGAACAGTGTATCCCCCTCTTTCATCTGGAACAGCTCATGCTGGAGCTTCAGTAGACTTAGCTATTTTTTCTTTACATTTAGCAGGAATTTCATCAATTTTAGGTGCAGTAAATTTTATTACAACAGTAATTAATATACGATCTTCAGGAATTACTCTTGATCGAATACCTTTATTTGTTTGATCAGTAGTAATTACTGCAGTTTTATTACTTCTTTCTTTACCTGTTTTAGCTGGTGCTATTACTATGTTATTAACAGATCGAAATTTAAATACTTCATTCTTTGATCCAATTGGAGGAGGAGATCCAATTTTA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/O-trivitattus.jpeg,",39.8309210000,-77.2388020000,09/23/2024,"
Suburban neighborhood<\/p>\n",Aedes trivittatus,Blood and Tissue kit,Abdomen,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-08-at-8.14.09 AM-5.png,","
Lane 8<\/p>\n",No,AAGATATTGGAACATTATACTTCATCTTTGGAGTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCA-GGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGATTAGTTCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATGGTAGAAAATGGATCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGAGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTTTTATCTCTCCCTGTATTAGCCGGAGCTATTACAATATTATTAACTGACCGAAATCTAAATACATCTTTCTTTGACCCAATTGGTGGAGGAGACCCAATTCTATATCAACATTTATTCTGATTTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Chris-jax-trivitattus-1.jpeg,",39.8625490000,-77.2751120000,09/20/2024,"
Rural area near a wetland<\/p>\n",Aedes trivittatus,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-08-at-8.14.09 AM-5.png,","
Lane 7<\/p>\n",No,GATATTGGAACATTATACTTCATCTTTGGAGTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGATTAGTTCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATGGTAGAAAATGGATCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGAGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTTTTATCTCTCCCTGTATTAGCCGGAGCTATTACAATATTATTAACTGACCGAAATCTAAATACATCTTTCTTTGACCCAATTGGTGGAGGAGANCCAATTCTATATCAACATTTATTCTGATTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Tonoy-Yunus-Culex-erraticus.jpeg,",39.862549,-77.275112,09/20/2024,"
Rural area near a wetland<\/p>\n",Culex erraticus,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-08-at-8.14.09 AM-5.png,","
Lane 6<\/p>\n",No,TATTGGAACATTATATTTTATTTTTGGGGCTTGAGCAGGAATAGTTGGAACATCTCTTAGTATTTTAATTCGAGCAGAATTAAGTCAACCTGGAAACTTTATTGGAAATGATCAAATTTATAATGTTATTGTAACAGCACATGCTTTTATTATAATTTTTTTTATAGTTATGCCTATTATAATTGGAGGATTTGGAAACTGATTAGTTCCTTTAATATTAGGGGCACCAGATATAGCATTCCCTCGAATAAATAATATGAGTTTTTGAATACTACCACCGTCATTAACTTTATTATTATCAAGTAGTTTAGTAGAAAATGGGGCTGGAACTGGATGAACTGTTTATCCTCCCCTTTCTTCTGGAACAGCACACGCAGGAGCTTCCGTTGATTTAGCAATTTTTTCTTTACACTTAGCAGGAATTTCTTCTATTTTAGGGGCCGTAAATTTCATTACTACAGTTATTAATATACGATCATCTGGTATTACTTTAGACCGAATACCTTTATTTGTTTGATCAGTAGTAATTACTGCAGTACTATTATTACTATCTTTACCAGTATTAGCCGGAGCTATTACTATACTTTTAACAGATCGAAATTTAAATACATCTTTCTTTGACCCTATTGGAGGAGGAGACCCTATTTTATATCAACACTTATTTTGATTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Culex-pipiens.jpeg,",39.830921,-77.238802,09/21/2024,"\n\n\n<\/colgroup>\n\n\n
<\/td>\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n",Culex pipiens,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-08-at-8.14.09 AM-4.png,","
Lane 5<\/p>\n",No,"GATATTGGAACATTATATTTTATTTTTGGGGCTTGAGCTGGAATAGTTGGAACTTCTTTAAGTTTACTAATTCGAGCAGAATTAAGTCAACCAGGTGTATTTATTGGAAATGATCAAATTTATAATGTTATTGTAACTGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCAATCATAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATGTTAGGAGCTCCAGATATGGCCTTTCCTCGAATAAATAATATAAGTTTTTGAATACTACCTCCTTCATTGACACTACTACTTTCAAGTAGTTTAGTAGAAAATGGAGCTGGGACTGGATGAACAGTGTATCCCCCTCTTTCATCTGGAACAGCTCATGCTGGAGCTTCAGTAGACTTAGCTATTTTTTCTTTACATTTAGCAGGAATTTCATCAATTTTAGGTGCAGTAAATTTTATTACAACAGTAATTAATATACGATCTTCAGGAATTACTCTTGATCGAATACCTTTATTTGTTTGATCAGTAGTAATTACTGCAGTTTTATTACTTCTTTCTTTACCTGTTTTAGCTGGTGCTATTACTATGTTATTAACAGATCGAAATTTAAATACTTCATTCTTTGATCCAATTGGAGGAGGAGATCCAATTTTATATCAACATTTATTTTGATTTTT ",,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Morgan-O-trivitattus.jpeg,",39.8625490000,-77.2751120000,09/19/2024,"
Caught in a rural area, near a wetland<\/p>\n",Aedes trivittatus,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-08-at-8.14.09 AM-3.png,","
Lane 4 in gel<\/p>\n",No,AAGATATTGGAACATTATACTTCATCTTTGGAGTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGATTAGTTCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATGGTAGAAAATGGATCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGAGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTTTTATCTCTCCCTGTATTAGCCGGAGCTATTACAATATTATTAACTGACCGAAATCTAAATACATCTTTCTTTGACCCAATTGGTGGAGGAGACCCAATTCTATATCAACATTTATTCTGATTTTTTG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Ella-Culex-erraticus.jpeg,",39.862549,-77.275112,09/19/2024,"
Lane 3 in gel. Collected from a rural area near a wetland<\/p>\n\n\n\n<\/colgroup>\n\n\n
<\/td>\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n",Culex erraticus,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-08-at-8.14.09 AM-2.png,","
Lane 3 in gel<\/p>\n",No,GATATTGGAACATTATATTTTATTTTTGGGGCTTGAGCAGGGATAGTTGGAACATCTCTTAGTATTTTAATTCGAGCAGAATTAAGTCAACCTGGAAACTTTATTGGAAATGATCAAATTTATAATGTTATTGTAACAGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAACTGATTAGTTCCTTTAATATTAGGAGCACCAGATATAGCATTCCCTCGAATAAATAATATAAGTTTTTGAATACTACCACCGTCATTAACTTTATTATTATCAAGTAGTTTAGTAGAAAATGGGGCTGGAACTGGATGAACTGTTTATCCTCCCCTTTCTTCTGGAACAGCACATGCAGGAGCTTCCGTTGATTTAGCAATTTTTTCTTTACACTTAGCAGGAATTTCTTCTATTTTAGGGGCCGTAAATTTCATTACTACAGTTATTAATATACGATCATCTGGTATTACTTTAGATCGAATACCTTTATTTGTTTGATCAGTAGTAATTACTGCAGTACTATTATTATTATCTTTACCAGTATTAGCCGGAGCTATTACTATACTTTTAACAGATCGAAATTTAAATACATCTTTCTTTGACCCTATTGGAGGAGGAGACCCTATTTTATATCAACACTTATTCTGATTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Chris-jax-trivitattus.jpeg,",39.8625490000,-77.2751120000,09/19/2024,"
Rural area near wetland<\/p>\n",Aedes trivittatus,DNeasy (Qiagen),,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-08-at-8.14.09 AM-1.png,","
Lane 2 in gel<\/p>\n",No,AGATATTGGAACATTATACTTCTTCTTTGGAGTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGATTAGTTCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATGGTAGAAAATGGGTCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGAGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTTTTATCTCTTCCTGTATTAGCCGGAGCTATTACAATATTGTTAACTGACCGAAATCTAAATACATCCTTCTTTGACCCAATTGGTGGAGGAGACCCAATTCTATATCAACATTTATTCTGATTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Jax-Aedea-albopictus.jpeg,",39.8309210000,-77.2388020000,09/21/2024,"
Cuaght in suburban neighborhood<\/p>\n",Aedes albopictus,DNeasy (Qiagen),,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-08-at-8.14.09 AM.png,","
Lane 1 in gel<\/p>\n",Yes,ATATTGGAACATTATACTTTATTTTCGGTATTTGATCTGGAATAGTCGGAACTTCACTAAGAGTTTTAATTCGTATTGAACTTAGACATCCTGGTATATTTATTGGAAATGATCAAATTTATAATGTAATTGTTACTGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCTATCATAATTGGAGGATTTGGAAACTGACTAGTACCCTTAATACTAGGAGCCCCTGATATAGCTTTTCCTCGAATAAATAATATAAGTTTTTGAATATTACCCCCCTCTTTAACACTGCTGCTTTCTAGTTCTATAGTAGAAAACGGAGCTGGAACAGGGTGAACGGTTTATCCTCCTCTTTCTTCTGGAACAGCTCATGCTGGGGCTTCAGTTGATTTAGCAATTTTTTCTTTACATTTAGCGGGAATCTCATCTATTTTAGGAGCAGTAAATTTTATTACAACTGTAATTAATATACGATCAGCTGGTATTACTCTTGATCGACTACCTTTATTTGTGTGATCAGTAGTAATTACAGCTATTTTATTACTTCTTTCTCTACCCGTATTAGCCGGAGCTATTACTATATTATTAACAGACCGAAATTTAAATACATCTTTTTTTGATCCAATTGGAGGGGGAGACCCTATTTTATATCAACATTTATTTTGANTTTT,,, "",19.68131123723556,-155.10209982442979,11/13/2024,"",Insecta,,Whole arthropod,,lysis,,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Gel-4-Top-1-scaled.jpg,","
the bottom one is mine<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/processed-C1144FCB-53C3-4E5E-B3D6-CC7D7565CEBA.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/processed-ECD7A8E1-F6FB-4504-BF87-9DC9D1DABD07.jpeg,",41.7756400000,-88.0970000000,09/03/2024,"
Collected by fall 2024 students.<\/p>\n",Dioxyna picciola,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-9.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/8D8BA2B9-B67D-46FB-9EA0-0911D521BAD9.jpg,",41.77766637781975,-88.09581357070628,08/28/2024,"
It was found near white flowers in the grassland area near the parking\u00a0 lot<\/p>\n
it’s red and spotted<\/p>\n
looks like a ladybug<\/p>\n",Coleoptera,pcr,Abdomen,Edvotek Gel Electrophoresis,,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_9137.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_3474-scaled.jpeg,",41.77564,-88.0970,09/04/2024,"
Collected by Fall 2024 students<\/p>\n",Harmonia axyridis,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_3684-1.jpeg,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/80-C_AB4_Co1R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/ladybugreal.jpg,",41.7784418000,-88.0952683000,09/04/2024,"
collected by Fall 2024 students<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/gel-1.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/marshfly-photograph.jpg,",41.7756400000,-88.0970000000,09/03/2024,"",Dictya floridensis,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/gele.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/FFD.jpg,",41.7759232000,-88.0967680000,09/03/2024,"",Ischnura posita,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-01-220252.png,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/51-A_AB1_Co1R.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Wasp-Image-Front.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Wasp-Image-Back.png,",41.77580142430422,-88.09727283016238,09/03/2024,"
collected by Fall 2024 students<\/p>\n",Polistes fuscatus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/tempImagen7A9Hy.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/WhatsApp-Image-2024-12-01-at-12.00.40-PM-7.jpeg,",41.7756400000,-88.0970000000,09/04/2024,"
The Asian lady bettle (hamornia axyridis) <\/em>observed was around 5-7mm in size with a dome shaped body and varing orange to red colors,marked with just 2 balck spots on sideways mostly spotless.It was found in the tree in my school by birch hall science building .The beetle moved slowly and stayed in the leaves for long time,displaying defensive behavior by secreting a yellow liquid when distrubed.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","
For gel electrophoresis: Prepare Agarose gel: dissolve agarose in TAE buffer heat,pour into a casting tray and let it soldify,mix DNA\/protein samples with loading buffer and add a molecular marker.Place the gel in the electrophoresis chamber ,load the samples and run at 100-150V for 30-60 minutes,Stain with ethidium bromide or coomassive brillant blue and view under UC light,for results compare the bands to the molecular marker to estimate fragment sizes.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_0368.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_0367.png,",38.9585852000,-77.0390598000,09/23/2024,"
The Arthrpod was caught in a house’s basement. It is very large and features long, spindly legs. It looks kind of like a grasshopper. It was very large. It seemed to do very well with dark spaces in an urban house’s basement. It was fall, and the house was 75 degrees Fahrenheit.<\/p>\n",Orthoptera,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_0375-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0393.png,","
DNA Extraction<\/p>\n
\n
The DNA extraction went well for the most part. The only issues of note are that we added about 50ul of extra Buffer to B-2, B-3, and B-5. We also spilled a little bit of B-5.<\/li>\n<\/ol>\n
PCR\u201d:<\/p>\n
\n
There was one notable mistake during the PCR, we forgot to switch pipette tips between samples possibly contaminating them.<\/li>\n<\/ol>\n
Gel electrophoresis, Arthropod CO1<\/p>\n
\n
The ladder was marked on all Gels<\/li>\n
Samples B-1 through B-6 were positive for arthropod DNA<\/li>\n
The positive and negative controls were also marked positive and water was marked negative<\/li>\n<\/ol>\n
Gel electrophoresis, Wolbachia<\/p>\n
\n
B-1 through B-5 were negative and B-6 was positive<\/li>\n
The controls worked with both negative and water controls marked negative and the positive control marked positive<\/li>\n
There were no notable mistakes or errors<\/li>\n<\/ol>\n",No,NNnTCATTAAGTATCTTAATCCGAACAGAACTAGGCCAACCAGGCTATTTAATTGGGGATGATCAAACTTATAACGTTAT TGTAACCGCACATGCATTTATCATGATTTTCTTTATAGTTATACCTATTATAATTGGGGGATTTGGAAATTGATTAGTTC CATTAATATTAGGAGCTCCAGATATAGCATTTCCACGAATAAATAATATAAGATTTTGACTTCTACCCCCGTCATTAACC CTTTTATTAACCAGAAGAATAGTCGAAAATGGTGCAGGAACAGGATGAACAGTTTATCCACCTTTATCAACAGGAATTGC TCATGCAGGAGCATCTGTTGATTTAGCTATTTTCTCGCTACATTTAGCAGGAATTTCCTCAATTTTAGGAGCTGTAAATT TTATTACTACCATAATTAATATACGAGCACCAGGAATATCATTAGATCAAACGCCATTATTTGTTTGAGCAGTTGGAATT ACAGCTCTTCTATTATTATTATCACTACn,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/B-5-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_0374.png,",38.9556760544,-77.0389772803,09/22/2024,"
This arthropod seems to be a small moth. It was quite small, but when it was flying around, it had a larger appearance. I was able to catch it right after it died. I caught it in a bedroom in a house. It was a very bright space, so it makes sense that the moth would be attracted to the light. It was an urban environment, 75f, and the season was fall.<\/p>\n",Lepidoptera,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_0375-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0393.png,","
DNA Extraction<\/p>\n
\n
The DNA extraction went well for the most part. The only issues of note are that we added about 50ul of extra Buffer to B-2, B-3, and B-5. We also spilled a little bit of B-5.<\/li>\n<\/ol>\n
PCR\u201d:<\/p>\n
\n
There was one notable mistake during the PCR, we forgot to switch pipette tips between samples possibly contaminating them.<\/li>\n<\/ol>\n
Gel electrophoresis, Arthropod CO1<\/p>\n
\n
The ladder was marked on all Gels<\/li>\n
Samples B-1 through B-6 were positive for arthropod DNA<\/li>\n
The positive and negative controls were also marked positive, and water was marked negative<\/li>\n<\/ol>\n
Gel electrophoresis, Wolbachia<\/p>\n
\n
B-1 through B-5 were negative and B-6 was positive<\/li>\n
The controls worked with both negative and water controls marked negative, and the positive control marked positive<\/li>\n
There were no notable mistakes or errors<\/li>\n<\/ol>\n",Yes,nAGTAGGAACATCCCTAAGATTACTAATTCGTGCTGAATTAGGAACCCCCGGATCTTTAATTGGTGATGATCAAATTTAT AATACTATTGTTACAGCTCATGCATTTATTATAATTTTTTTTATAGTAATACCAATTATAATTGGTGGATTTGGAAATTG ATTAGTTCCATTAATACTAGGAGCACCTGATATAGCATTCCCACGAATAAATAATATAAGATTTTGATTATTGCCTCCCT CTTTAACCTTATTAATTTCTAGAAGAATTGTAGAAAATGGGGCTGGAACAGGATGAACAGTATACCCCCCCCTTTCATCT AATATTGCTCACAGAGGAAGATCTGTAGACTTAGCTATTTTTTCCCTACACTTAGCAGGAATTTCTTCAATTCTAGGAGC AATTAATTTTATTACAACTATTATTAACATACGAATTAATGGATTATCTTTTGATCAAATACCTTTATTTGTTTGATCTG TTGGAATTACAGCTTTACTTCTTCTCCTATCATTACCAGTATTAGCTGGAGCTATTACTATATTACTTACTGATCGAAAT CTTAATACTTCTTTTTTn,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/B-6-CO1F-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_7466-scaled.jpeg,",20.1151832212,-155.5577569263,11/12/2024,"
Most likely to be Aedes albopictus or Asian Tiger Mosquito <\/em>due to the fact that these are day time mosquitos and these were caught in the afternoon when there was still a decent amount of light left.<\/p>\n",Aedes albopictus,pcr,Whole arthropod,Standard electrophoresis system,lysis,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-05-at-2.25.42 PM-e1733444788992.png,","
Agarose gel used was created using a 2% Agarose mixture.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/tempImage7VF8LT-scaled-e1733446266653.jpg,",20.1153336655,-155.5581018466,11/12/2024,"
From my observations the species of mosquito I collected was most likely Aedes albopictus <\/em>or Asian Tiger Mosquito.<\/p>\n",Aedes albopictus,pcr,Whole arthropod,Standard electrophoresis system,lysis,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-05-at-1.12.50 PM-1.png,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Red-Fire-Ant-scaled.jpg,",19.7014773000,-155.0786358000,11/14/2024,"
Red Fire Ants are pretty small so this did concern me if the DNA would be effectively amplified to be shown in the end results. But it’s size and body structure made DNA extraction fairly easy.<\/p>\n",Arthropoda,pcr,Whole arthropod,Standard electrophoresis system,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-11-24-at-3.23.38 PM-2.png,","
Gel Lanes:<\/p>\n
\n
DNA ladder<\/li>\n
Fire ant 1 with CO1 primers<\/li>\n
Fire ant 2 with CO1 primers<\/li>\n
Fire ant 1 with Wolbachia 16S primers<\/li>\n
Fire ant 2 with Wolbachia 16S primers<\/li>\n<\/ol>\n
Positive and negative controls are not shown, but they both worked.<\/p>\n
Experimental Set Up<\/p>\n
\n
Sample Collection, two Red Fire Ants were placed into tubes<\/span><\/li>\n
Cell Lysis, cell lysis buffer was added to each tube, mash for 1 minute, add another 100 \u00b5L, and mash again.<\/span><\/li>\n
Heat Treatment, tubes were sealed with parafilm and heated for 5 minutes.<\/span><\/li>\n
DNA Precipitation Setup, added cold isopropanol to each tube for DNA precipitation.<\/span><\/li>\n
Cooling and Centrifugation, samples were cooled to room temperature, vortexed, then centrifuged.<\/span><\/li>\n
Supernatant Transfer, transferred 150 \u00b5L of supernatant to new tubes with isopropanol, then centrifuge for 5 minutes.<\/span><\/li>\n
Washing, supernatant was discarded, pellets air dried (DNA), then<\/span> ethanol was added and centrifuged<\/span><\/li>\n
Arthropod PCR of Insect 15, Arthropod PCR of Insect 16, Wolbachia<\/em>, PCR of Insect 1, Wolbachia<\/em> PCR of Insect 2 were placed into gel loading tray and into the<\/span> electrophoresis machine<\/span><\/li>\n<\/ul>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/tempImagetkZQs1-scaled.jpg,",19.6419129000,-155.9962255000,11/14/2024,"",Lasius niger,pcr,Whole arthropod,Standard electrophoresis system,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/tempImagefQCJS3-scaled.jpg,","
Gel Lanes (Top gel)<\/p>\n
\n
DNA ladder<\/li>\n
Sample 1 CO1<\/li>\n
Sample 2 CO1<\/li>\n
Sample 1 16S<\/li>\n
Sample 2 16S<\/li>\n<\/ol>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/FF06C964-EBA3-4EA8-B183-DAFA4E751698_1_105_c.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/B0A399DE-D7B4-4345-B9BE-377FEAA00ABC_1_105_c.jpeg,",41.7756400000,-88.0970000000,09/04/2024,"
Collected by Fall 2024 students (sweep net)<\/p>\n",Odonata,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",Unknown,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/75-C_AG2_Co1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/20241114_140038-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/20241202_223411-scaled.jpg,",19.6921345203,-155.0618383757,11/13/2024,"
During the experiment after smashing the insect with the sterile pestle and adding the sample into the centrifuge there was still a giant pellet at the bottom of the test tube. I returned the sample to the centrifuge and the giant pellet still remained. I Pipetted out as much as I could without disturbing the pellet and even after the third round in the centrifuge and other experimental steps the pellet still remained.<\/p>\n",Tipulidae,pcr,body without wings,Standard electrophoresis system,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Gel-3_RayKalei.jpg,","
Summary of events<\/i><\/b>: During the experiment I was briefly educated by my classmates that the bug I had was NOT a mosquito but a cane fly. That did not change the fact that I did have to dissect the bug and tear apart its little legs and wings using tweezers. After the budget dissection I added the bug torso to a test tube and added, cell lysis buffer. Using a sterile pestle I crushed the bug in the test tube making sure to push it hard against the bottom twisting as I pushed, then I\u00a0 dragged it up against the side of the test tube making sure it was thoroughly squished but parts of the bug did get stuck on the pestle and I did have to rub it off on the rim of the test tube to get it off. In the process the cell lysis buffer bubbled and disappeared a little while the otherwise clear lysis buffer coloring was brown and murky. Added the test tube to the cooling block, set my phone timer for 5 minutes and waited. After 5 minutes I collected my test tube and tilted my test tubes back and forth for a few minutes. I added them to the mini centrifuge for 2 minutes and began preparing my isopropanol test tube by pipetting and marking the test tube. After the two minutes finished I started to pipette the supernatant out of the test tube and into another test tube. There were some issues where some of the bug parts dislodged from the bottom of the test tube and I needed to put it back in the centrifuge for another 2 minutes. After that there were no issues collecting the supernatant. From there I added the supernatant to the isopropanol and mixed them together by once again flipping the test tube a few times. After that I placed the newly mixed tube into the centrifuge once again but this time for 5 minutes. After the time was up I collected my test tube and noticed that there was a large visible pellet at the bottom of the tube. It did not move and wouldn’t dislodge in the final steps. I pipetted the liquid out of the test tube and into a small waste beaker and balanced the test tube upside down on a paper towel for a few minutes. Afterwards, I added more isopropanol to the test tube, flipped it back and forth some more to mix it once again and put it back into the centrifuge for a minute. After a minute I removed the test tubes from the centrifuge and added some DNA elution buffer, labeled my test tubes and handed it off to the instructor.\u00a0<\/i><\/b><\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_8916-scaled-e1733425843486.jpeg,",19.6933231,-155.1298895,11/13/2024,"
One seemed dead when I caught it, and the other was alive near Kaumana caves.<\/p>\n",Culicidae,none,Whole arthropod,,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-05-at-9.36.53 AM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_0543-1-rotated.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-05-074759.png,",19.6419129000,-155.9962255000,11/16/2024,"
\n
Ants were very easy to squish.<\/li>\n
A light red\/orange yellow color.<\/li>\n
Smaller than red ants.<\/li>\n<\/ul>\n",Formicidae,tweezers,Whole arthropod,MiniPCR,TBE,Other,"","
Gel lanes (Bottom gel)<\/p>\n
\n
Sample 1 CO1<\/li>\n
Sample 2 CO1<\/li>\n
Sample 1 16S<\/li>\n
Sample 2 16S<\/li>\n<\/ol>\n
<\/p>\n",Yes,#2 is on 6 = 700 bp. Seems to be the clearest. #3 is on 6 = 700 bp.,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_5290-scaled.jpeg,",44.9295514393,-93.1741586091,10/12/2024,"",Hemiptera,Qiagen Blood and Tissue kit,Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/Screenshot-2025-04-01-115017.png,","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/image0-1-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/image1-scaled.jpeg,",,,11/22/2024,"
$\"\"$ $\"\"$ $\"\"$ $\"\"$ $\"\"$ <\/p>\n",Arthropoda,pcr,Whole arthropod,Edvotek Gel Electrophoresis,TBE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/image-1-1.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_2728-1.jpg,",19.5694612243,-155.1123242500,11/13/2024,"
Fire ant was captured using peanut butter in a vile. A small quantity of peanut butter; no more than pea-sized was placed in a vile and left for an hour. Over 20 specimens were collected.<\/p>\n
This site gave me goosebumps.<\/p>\n",Wasmannia auropunctata,tweezers,Whole arthropod,Standard electrophoresis system,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Gel-1-top-row_Kahiau-scaled.jpg,","
The extraction methods include washing the specimen, drying it, then using a pestle and lysis buffer to break it up. Then I heated the tubes, cooled them, centrifuged it then separated the debris and transferred the supernatant to a new tube. The supernatant was then combined with isopropanol, mixed, and centrifuged to get a pellet. The pellet was then washed with ethanol, and then air dried. The DNA pellet was then resuspended in the elution buffer and incubated at 65 degrees C. It was then stored at -20 degrees C.<\/p>\n
To set up the PCR we would have put on PPE and cleaned the work station. We then would have labeled the DNA we extracted as well as the PCR tubes with t=our initials and sample numbers.<\/p>\n
<\/p>\n
For the CO1 gene we would have used a mixture containing taq polymerase, CO1 primers, dNTPs, and buffers. Then adding 2 ul of DNA into the mixtures in the labeled PCR tubes. The lids were then secures, centrifuged, then placed in the PCR rack. The PCR cycle for CO1 consists of an initial denaturation for 2 minutes at 94 degrees C, followed by 30 cycles of 30 seconds at 94 degrees, annealing for 45 seconds at 49 degrees C and then extension for 60 seconds at 72 degrees C.Then it was extended for 10 minutes at 72 degrees for the final extension.<\/p>\n
<\/p>\n
For the Wolbachia sample we would have used PCR mixture containing taq polymerase, Wspec primers, dNTPs, and buffers. We would have added 2 uL of DNA sample into the labeled PCR tubes, centrifuged them, then placed them on the PCR rack. The PCR cycle would have then been performed the same with the exception of the annealing stage which was 45 seconds at 55 degrees Celcius. The samples were then stored in the freezer and the workstations were cleaned.<\/p>\n
As always this began by donning PPE and cleaning the work stations. The casting tray was then prepared by putting the rubber caps on each end and inserting the combs. The agarose solution was then prepared by mixing 1 gram of agarose powder, 50 mL of 1X TBE buffer, and dissolved by heating and swirling the mixture to make a 2% gel to create the optimal sized pores for this electrophoresis test. The gel was then cooled before adding 2.5 uL of SYBR Safe DNA stain into the gel mixture. We then poured the mixture into the casting block and allowed it to cool. The electrophoresis was then set up by removing the gel block and placing it into the electrophoresis chamber and submerging it with 300 mL of TBE buffer. We then created a loading key table listing the lanes and the sample IDs. The gel was then ready to be loaded and ran.<\/p>\n
To run the agarose gel we first practiced loading. This step is optional. We then prepared the sample by placing a piece of parafilm on the bench and labeling it with the sample IDs. We then added 2 uL of 6x DNA loading dye to the designated parafilm and mixed 10 uL of DNA ladder or PCR samples with the loading dye. The DNA ladder and samples were then loaded into the wells ensuring to keep track of which sample went in each well\/lane.<\/p>\n
<\/p>\n
Following this, we ran the gel by connecting the negative and positive electrodes to the power supply. We then ran the gel at 130 volts for 30 minutes.<\/p>\n
<\/p>\n
After the electrophoresis was completed, we turned off the power and disconnected the electrophoresis device. The gel was then placed on a UV light transilluminator and analyzed using eye protection to observe the results of the test. The results were then recorded and the stations were cleaned up.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-04-185833.png,",19.1160616000,-155.7821510000,11/13/2024,"",Insecta,pcr,Abdomen,Standard electrophoresis system,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-04-190448.png,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_7235-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_7230-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_7232-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_7224-scaled.jpeg,",41.7756400000,-88.0970000000,09/03/2024,"
collected by Fall 2024 students, I collected this specimen with a jar-and-lid method on a milkweed plant by a pond.<\/p>\n",Oncopeltus fasciatus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_7795-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_7794-scaled.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/TLM-scaled.jpg,",33.9886173569,-84.4400379179,11/12/2024,"
Has a darkish brown\/black exoskeleton with orange\/red ends. Most body segments appear to contain two pairs of white colored legs. The two white antennae can be seen at the anterior end of the millipede with the mouth and mandible inferior to the antennae.<\/p>\n",Euryurus leachii,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/8-wol-1.jpeg,","
Lane 1 includes the DNA marker of double-stranded DNA fragment sizes 10k, 6k, 3k, 2k, 1k, 800, 600, 400, and 200 bp. The Gel shown is of the Wolbachia protocol, testing for the 16s rRNA gene only using specific primers. Lane 7 is of my leach’s train millipede arthropod sample, with the negative arthropod control in lane 9 and the negative DNA control in lane 10. The positive arthropod control and the positive DNA control were not run in the Wolbachia protocol gel due to errors during preparation. The Arthropod protocol gel is not shown due to unclear results.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_3884-scaled.jpg,",19.7080976000,-155.0715471000,11/11/2024,"
– The beetle was quite large and robust
\n– It had a dark outer hard shell with white speckles
\n– It had wings and was able to fly, but very slowly
\n– I found it eating my compost<\/p>\n",Amithao decemguttatus,pcr,Partial abdomen,Standard electrophoresis system,TBE,SYBR Safe,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG-5820-1.jpg,",19.4899412442,-154.9366077909,11/12/2024,"
moved slowly when caught, couldn’t fly properly, red eyes<\/p>\n",Diptera,pcr,Abdomen,Standard electrophoresis system,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-11-24-5.03.51-PM.png,","
Lane 1 Arthropod PCR of Insect 1<\/p>\n
Lane 2 Arthropod PCR of Insect 2<\/p>\n
Lane 3 Wolbachia PCR of Insect 1<\/p>\n
Lane 4 Wolbachia PCR of Insect 2<\/p>\n
Lane 5 DNA ladder<\/p>\n
<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/04A7D987-3013-4131-A10F-7E3074E857F1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_6814-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_6816-scaled.jpeg,",41.7756400000,-88.0970000000,09/04/2024,"
collected by Fall 2024 students by aspirator in grassy land by a pond<\/p>\n",Isopoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/82-C_AV2_Co1R-1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-02-at-12.55.39 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-02-at-12.55.29 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-02-at-12.56.08 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-02-at-12.55.57 PM.png,",41.7756400000,-88.0970000000,09/04/2024,"
The insect was caught near Lake Saint Benedict on a bush using a plastic jar.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-02-at-12.53.46 PM.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/D2.jpeg,",41.6763200000,-77.0695500000,08/31/2024,"
Location – Moist dirt in thick brush, on small stick<\/span><\/p>\n
Notes – Dangling from silk <\/span><\/p>\n",Naphrys pulex,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/D2.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/C2.jpeg,",41.6759600000,-77.0677900000,08/31/2024,"
Location – Moist dirt in thick brush<\/span><\/p>\n
Notes – Dirt was cool to the touch<\/span><\/p>\n",Philoscia muscorum,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/C2.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_0989-scaled.jpeg,",44.9259603210,-93.1736667155,09/12/2024,"
it was an ant, it was small and blackish.<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniPCR,TBE,GelGreen,"","
Many samples from different arthropods run in gel together<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Mosquito-2.jpeg,",39.8625490000,-77.2751120000,09/19/2024,"
C01- AAGATATTGGAACATTATACTTCATCTTTGGAGTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGACTAGTTCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATAGTAGAAAATGGATCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGGGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTTTTATCTCTCCCTGTATTAGCCGGAGCTATTACAATATTATTAACTGACCGAAATCTAAATACATCTTTCTTTGACCCAATTGGTGGAGGAGATCCAATTCTATATCAACATTTATTCTGATTTTTTG<\/span><\/p>\n",Aedes trivittatus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-02-at-3.26.42 PM-2.png,","",No,AAGATATTGGAACATTATACTTCATCTTTGGAGTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGACTAGTTCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATAGTAGAAAATGGATCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGGGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTTTTATCTCTCCCTGTATTAGCCGGAGCTATTACAATATTATTAACTGACCGAAATCTAAATACATCTTTCTTTGACCCAATTGGTGGAGGAGATCCAATTCTATATCAACATTTATTCTGATTTTTTG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/824CD5E6-1583-4BC7-9D3F-CAC44C0A5090.jpeg,",39.8625490000,-77.2751120000,09/20/2024,"
CO1: AAAGATATTGGAACATTATACTTCATCTTTGGAGTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGATTAGTTCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATGGTAGAAAATGGATCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGAGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTTTTATCTCTTCCTGTATTAGCCGGAGCTATTACAATATTGTTAACTGACCGAAATCTAAATACATCCTTCTTTGACCCAATTGGTGGGGGAGACCCAATTCTATACCAACATTTATTTTGATTTTTTG<\/span><\/p>\n",Aedes trivittatus,Qiagen Blood and Tissue kit,Abdomen,Standard electrophoresis system,LB,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screen-Shot-2024-12-02-at-3.18.05-PM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/large.jpeg,",39.8625490000,-77.2751120000,09/19/2024,"
CO1-<\/p>\n
\n
\n
ANTGGATCTCCTCCNCCAATTGGGTCAAAGAAAGATGTATTTAGATTTCGGTCAGTTAATAATATTGTAATAGCTCCGGCTAATACAGGGAGAGATAAGAGTAATAAAACAGCTGTAATAACAACAGATCAAACAAATAATGGTAATCGATCTAAAGTAATTCCTGCAGATCGTATATTAATAAGAATAGTAATAAAATTTACAGCTCCTAAAATTGATGATACTCGTGCTGAATGAAGAGAAAAAATAGTTAAGTCAACAGAAGCTCCAGCATGAGCAGTTCCAGATGAAAGAGGGGGATAAACAGTTCATCCTGTTCCTGATCCATTTTCTACCATACTACTTGAAAGTAGTAGAGTTAATGAAGGAGGTAATATTCAGGAACTTATATTATTTATTCGAGGGAAAGCTATATCTGGTGCTCCTAGTATAAGAGGAAATAATCAATTTCCGAATCCTCCAATTATAATAGGTATAACTATAAAGAAAATTATAATAAAAGCATGAGCTGTAACAATTACATTATAAATTTGGTCATTTCCAATAAATATTCCTGGTTGACTTAATTCAGCACGAATTAAAATTCTTAATGATGTACCAACTATTCCTGATCAAACTCCAAAGATGAAGTATAATGTTCCAATATCTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATCCTCCAATTATAATTTTCTTTATAGGTATAACTATAATAATTGGAGGATTCAGAAATTGATTAGTTCCTATTATAATAGGAGCATGAGCTGTAACTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATGGTAGAAAATGGATCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGAGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTCTTATCTCTCCCTGTATTAGCCGGAGCTATTACAATATTATTAACTGACCGAAATCTAAATACATCTTTCTTTGACCCAATTGGTGGAGGAAATCCAATTCTATATCAACATTTGTTCTGATATCTTG<\/div>\n<\/div>\n<\/div>\n",Aedes trivittatus,Qiagen Blood and Tissue kit,Abdomen,Standard electrophoresis system,TBE,GelGreen,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/M.jpeg,",39.8347335000,-77.2364739000,09/19/2024,"",Aedes,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/GEL.png,","",No,TAAAGATATTGGAACATTATACTTCATCTTTGGAGTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGATTAGTGCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATGGTAGAAAATGGATCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGAGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTTTTATCTCTCCCTGTATTAGCCGGGGCTATTACAATATTATTAACTGACCGAAATCTAAATACATCTTTCTTTGACCCAATTGGTGGAGGAGACCCAATTTTATATCAACATTTATTCTGATTTTTTGGTCAC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Mosquito-1.jpeg,",39.83567439999999,-77.2366796,09/23/2024,"
CO1<\/span><\/p>\n
AAAGATATTGGNACATTATACTTCATCTTTGGAGTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGATTAGTTCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATGGTAGAAAATGGATCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGAGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTCTTATCTCTCCCTGTATTAGCCGGAGCTATTACAATATTATTAACTGACCGAAATCTAAATACATCTTTCTTTGACCCAATTGGTGGAGGAGACCCAATTCTATATCAACATTTATTCTGATTTTTTGGTCACCC<\/span><\/p>\n",Aedes,Qiagen Blood and Tissue kit,Abdomen,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screen-Shot-2024-12-02-at-3.14.20-PM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/mosquito.jpeg,",39.8346640000,-77.2363029000,01/24/2025,"
co1<\/p>\n
AAGATATTGGAACATTATACTTCATCTTTGGAGTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGATTAGTTCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATGGTAGAAAATGGATCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGAGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTTTTATCTCTCCCTGTATTAGCCGGAGCTATTACAATATTATTAACTGACCGAAATCTAAATACATCTTTCTTTGACCCAATTGGTGGAGGAGACCCAATTCTATACCAACATTTATTCTGATTTTTTG<\/span><\/p>\n",Aedes trivittatus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Ryan-Kerney-2024-11-17_14h49m08s-1-scaled.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-02-at-3.00.47 PM.png,",39.8401617000,-77.2365022000,09/19/2024,"",Culex,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,GelGreen,"","",No,AAGATATTGGGACATTATATTTTATTTTTGGGGCTTGAGCAGGAATAGTTGGAACATCTCTTAGTATTTTAATTCGAGCAGAATTAAGTCACCCTGGAAACTTTATTGGAAATGATCAAATTTATAATGTTATTGTAACAGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAACTGATTAGTTCCTTTAATATTGGGGGCACCAGATATAGCATTCCCTCGAATAAATAATATAAGTTTTTGAATACTACCACCGTCATTGACTTTATTATTATCAAGTAGTTTAGTAGAAAATGGGGCTGGAACTGGATGAACTGTTTATCCACCCCTTTCTTCTGGAACAGCACATGCAGGAGCTTCCGTTGATTTAGCAATTTTTTCTTTACACTTAGCAGGAATTTCTTCTATTTTAGGGGCCGTAAATTTTATTACTACAGTTATTAATATACGATCATCTGGTATTACTTTAGATCGAATACCTTTATTTGTTTGATCAGTAGTAATTACTGCAGTACTATTATTACTATCTTTACCAGTATTAGCCGGAGCTATTACTATACTTTTAACAGATCGAAATTTAAATACATCTTTCTTTGACCCTATTGGAGGAGGAGATCCTATTTTATATCAACACTTATTTTGATTTTT AAGATATTGGGACATTATATTTTATTTTTGGGGCTTGAGCAGGAATAGTTGGAACATCTCTTAGTATTTTAATTCGAGCAGAATTAAGTCACCCTGGAAACTTTATTGGAAATGATCAAATTTATAATGTTATTGTAACAGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAACTGATTAGTTCCTTTAATATTGGGGGCACCAGATATAGCATTCCCTCGAATAAATAATATAAGTTTTTGAATACTACCACCGTCATTGACTTTATTATTATCAAGTAGTTTAGTAGAAAATGGGGCTGGAACTGGATGAACTGTTTATCCACCCCTTTCTTCTGGAACAGCACATGCAGGAGCTTCCGTTGATTTAGCAATTTTTTCTTTACACTTAGCAGGAATTTCTTCTATTTTAGGGGCCGTAAATTTTATTACTACAGTTATTAATATACGATCATCTGGTATTACTTTAGATCGAATACCTTTATTTGTTTGATCAGTAGTAATTACTGCAGTACTATTATTACTATCTTTACCAGTATTAGCCGGAGCTATTACTATACTTTTAACAGATCGAAATTTAAATACATCTTTCTTTGACCCTATTGGAGGAGGAGATCCTATTTTATATCAACACTTATTTTGATTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_0050-rotated.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_0049-rotated.jpeg,",39.8345372674,-77.2361062092,09/19/2024,"
CO1 Sequence:<\/p>\n
TCATAAAGATATTGGAACATTATACTTCATCTTTGGAGTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGATTAGTTCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATGGTAGAAAATGGGTCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGAGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTTTTATCTCTTCCTGTATTAGCCGGAGCTATTACAATATTGTTAACTGACCGAAATCTAAATACATCCTTCTTTGATCCAATTGGTGGGGGAGACCCAATTTTATATCAACATTTATTCTGATTTTTTGGTCACCC<\/p>\n",Aedes,Blood and Tissue kit,Abdomen,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Ryan-Kerney-2024-11-17_14h49m08s_6b56b8cf69b92d53591152523c452f08-Medium.jpeg,","",No,,,, "",39.8309293000,-77.2310955000,09/19/2024,"
\n
\n
co1 sequence-<\/p>\n
ANGATATTGGAACATTATATTTTATTTTTGGGGCTTGAGCAGGAATAGTTGGGACATCTCTGAGTATTTTAATTCGAGCAGAATTAAGTCAACCTGGAAACTTTATTGGAAATGATCAAATTTATAATGTTATTGTAACAGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAACTGATTAGTTCCTTTAATATTAGGAGCACCAGATATAGCATTCCCTCGAATAAATAATATGAGTTTTTGAATACTACCACCGTCATTAACTTTATTATTATCAAGTAGTTTAGTAGAAAATGGGGCTGGAACTGGATGAACTGTTTATCCTCCCCTTTCTTCTGGAACAGCACATGCAGGAGCTTCCGTTGATTTAGCAATTTTTTCTTTGCACTTAGCAGGAATTTCTTCTATTTTAGGGGCCGTAAATTTCATTACTACAGTTATTAATATACGATCATCTGGTATTACTTTAGATCGAATACCTTTATTTGTTTGATCAGTAGTAATTACTGCAGTACTATTATTACTATCTTTACCAGTATTAGCCGGAGCTATTACTATACTTTTAACAGATCGAAATTTAAATACATCTTTCTTTGACCCTATTGGAGGAGGAGACCCTATTTTATATCAACACTTATTCTGATTTTT<\/div>\n<\/div>\n<\/div>\n
<\/div>\n",Psorophora ferox,Blood and Tissue kit,Abdomen,Standard electrophoresis system,TBE,GelGreen,"","",No,,,, "",39.8309293000,-77.2310955000,09/19/2024,"
CO1 sequence:<\/div>\n
\n
\n
\n
AAAGATATTGGAACATTATATTTTATTTTTGGGGCTTGAGCAGGAATAGTTGGAACATCCCTTAGTATTTTAATTCGAGCAGAGTTAAGTCAACCTGGAAACTTTATTGGAAATGATCAAATTTATAACGTTATTGTAACAGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAACTGATTAGTTCCTTTAATATTAGGGGCACCAGATATAGCATTTCCTCGAATAAATAATATAAGTTTTTGAATACTACCACCGTCATTAACTTTATTATTATCAAGTAGTTTAGTAGAAAATGGAGCTGGAACTGGATGAACTGTTTATCCTCCCCTTTCTTCTGGAACAGCACATGCAGGAGCTTCCGTTGATTTAGCAATTTTTTCTTTACACTTAGCAGGAATTTCTTCTATTTTAGGGGCCGTAAATTTCATTACTACAGTTATTAATATACGATCATCTGGTATTACTTTGGATCGAATACCTTTATTTGTTTGATCAGTAGTAATTACTGCAGTACTATTGTTACTATCTTTACCAGTATTAGCCGGAGCTATTACTATACTTTTAACAGATCGAAATTTAAATACATCTTTCTTTGACCCTATTGGAGGAGGAGATCCTATTTTATACCAACACTTATTCTGATTTTTTGGTNC<\/p>\n<\/div>\n<\/div>\n<\/div>\n",Culex erraticus,DNeasy (Qiagen),Other,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-02-at-2.09.44 PM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-02-at-1.55.11 PM.png,",39.8309210000,-77.2388020000,09/19/2024,"
CO1 Sequence:<\/p>\n
ANGATATTGGAACATTATATTTTATTTTTGGGGCTTGAGCAGGAATAGTTGGGACATCTCTGAGTATTTTAATTCGAGCAGAATTAAGTCAACCTGGAAACTTTATTGGAAATGATCAAATTTATAATGTTATTGTAACAGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAACTGATTAGTTCCTTTAATATTAGGAGCACCAGATATAGCATTCCCTCGAATAAATAATATGAGTTTTTGAATACTACCACCGTCATTAACTTTATTATTATCAAGTAGTTTAGTAGAAAATGGGGCTGGAACTGGATGAACTGTTTATCCTCCCCTTTCTTCTGGAACAGCACATGCAGGAGCTTCCGTTGATTTAGCAATTTTTTCTTTGCACTTAGCAGGAATTTCTTCTATTTTAGGGGCCGTAAATTTCATTACTACAGTTATTAATATACGATCATCTGGTATTACTTTAGATCGAATACCTTTATTTGTTTGATCAGTAGTAATTACTGCAGTACTATTATTACTATCTTTACCAGTATTAGCCGGAGCTATTACTATACTTTTAACAGATCGAAATTTAAATACATCTTTCTTTGACCCTATTGGAGGAGGAGACCCTATTTTATATCAACACTTATTCTGATTTTT<\/p>\n",Culex erraticus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-02-at-2.09.29 PM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-02-at-1.57.55 PM.png,",39.8309293,-77.2310955,09/19/2024,"
\n
\n
AAGATATTGGAACATTATATTTTATTTTTGGGGCTTGAGCAGGAATAGTTGGAACATCTCTTAGTATTTTAATTCGAGCAGAATTAAGTCAGCCTGGAAACTTTATTGGAAATGATCAAATTTATAATGTTATTGTAACAGCACATGCTTTTATTATAATTTTTTTTATAGTTATGCCTATTATAATTGGAGGATTTGGAAACTGATTAGTTCCTTTAATATTAGGGGCACCAGATATAGCATTCCCTCGAATAAATAATATGAGTTTTTGAATACTACCACCGTCATTAACTTTATTATTATCAAGTAGTTTAGTAGAAAATGGGGCTGGAACTGGATGAACTGTTTATCCTCCCCTTTCTTCTGGAACAGCACATGCAGGAGCTTCCGTTGATTTAGCAATTTTTTCTTTACACTTAGCAGGAATTTCTTCTATTTTAGGGGCCGTAAATTTCATTACTACAGTTATTAATATACGATCATCTGGTATTACTTTAGATCGAATACCTTTATTTGTTTGATCAGTAGTAATTACTGCAGTACTATTATTACTATCTTTACCAGTATTAGCCGGAGCTATTACTATACTTTTAACAGATCGAAATTTAAATACATCTTTCTTTGACCCTATTGGAGGAGGAGACCCTATTTTATATCAACACTTATTTTGATTTTTTGG<\/div>\n<\/div>\n<\/div>\n
<\/div>\n",Culex erraticus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Screenshot-2024-12-02-at-2.08.59 PM.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_5731-scaled.jpeg,",39.8345300000,-77.2362200000,09/16/2024,"
CL1Strain: TAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGATTAGTTCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATGGTAGAAAATGGATCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGAGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTTTTATCTCTCCCTGTATTAGCCGGAGCTATTACAATATTATTAACTGACCGAAATCTAAATACATCTTTCTTTGACCCAATTGGTGGAGGAGACCCANTTCTATATCANCATTTATTCTGATTTTTTGGTCACCCTTGNAAGTTTTAA<\/p>\n",Aedes trivittatus,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Ryan-Kerney-2024-11-17_14h49m08s.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/bucky-mosquito-.jpeg,",39.8625490000,-77.2751120000,09/19/2024,"
CO 1 sequence: AAAGATATTGGAACATTATACTTCATCTTTGGAGTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGATTAGTTCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATGGTAGAAAATGGGTCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGAGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTTTTATCTCTTCCTGTATTAGCCGGAGCTATTACAATATTGTTAACTGACCGAAATCTAAATACATCCTTCTTTGACCCAATTGGNGNNNAGACCCAATTCTANNTCANCATTTATTCTGATTTTTGGT<\/span><\/p>\n",Aedes trivittatus,Qiagen Blood and Tissue kit,Abdomen,,TBE,GelGreen,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/vinnty-the-mosquito.jpeg,",39.8309293000,-77.2310955000,09/19/2024,"
CO1 sequence –<\/p>\n
\n
\n
ANTCATAAAGATATNGGAACATTATACTTCATCTTTGGAGTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGATTAGTTCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATGGTAGAAAATGGATCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGAGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTCTTATCTCTCCCTGTATTAGCCGGAGCTATTACAATATTATTAACTGACCGAAATCTAAATACATCTTTCTTTGACCCAATTGGTGGAGGAGATCCAATTCTATATCAACATTTATTCTGATTTTT<\/div>\n<\/div>\n<\/div>\n
<\/div>\n",Aedes trivittatus,Qiagen Blood and Tissue kit,Abdomen,,TBE,GelGreen,"","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_8144-scaled.jpeg,",41.7757397170364,-88.09599941220289,09/04/2024,"
Collected by Fall 2024 Students BenU<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_8436-scaled.jpeg,","",No,AGTTGAAATAAAATTAATAGCTCCTAAAATTGAAGATATACCCGCAATATGGAGGGAAAAAATAGTTAAATCAACTGAAG GTCCATTATGGAAAATATTTGAAGCTAAAGGAGGATAAACAGTTCATCCTGTCCCAACACCATCATTAATAAAATTTCTT AAAATAAGAAGAGAAATTGAGGGGGGTAAAAGTCAAAATCTCATATTGTTTATACGTGGATAAGCTATATCAGGTGAGCC TAATATTAAAGGTACAAGGAAATTT,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/12-A_WP1_WoF-araneta-wolbachia.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_3166-1-scaled.jpg,",41.7788628000,-88.0948979000,09/03/2024,"
Collected by Fall 2024 students. I collected my insect using a beat net in the bushes near the pond outside of Birck Hall.<\/p>\n",Ischnura perparva,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/IMG_4002-rotated.jpeg,","
I did not receive any DNA (insect or Wolbachia<\/em> DNA), but I believe my species is Ischnura perparva<\/em> (common name being Western Forktail in the Narrow-Winged Damselfly family) because of my Seek App results, and my knowledge on insect anatomy. Firstly, the Seek App clarified the order Odonata, the genus\u00a0Ischnura\u00a0<\/em>and then the species\u00a0Ischnura perparva<\/em>. Additionally, my knowledge with insect anatomy entails that the order Odonata has 3 body parts (head, thorax, abdomen), sucking mouth parts, antennae, 3 pairs of legs, 2 pairs of wings, and compound eyes, which are all anatomical characteristics of the Western Forktail.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_7937-scaled.jpeg,",41.7756400000,-88.0970000000,09/03/2024,"
Collected by Fall 2024 students.<\/p>\n",Hylephila phyleus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_9774-scaled.jpeg,","",Yes,AAAACAGGTAAAGATAATAATAATAATAATGCAGTAATACCTACAGATCATACAAATAATGGTATTTGATCAAATGATAA ATTTTTAATTCGTATATTAATAATTGTTGTAATAAAATTAATAGCTCCTAAAATAGATGAAATTCCAGCTAAATGAAGGG AAAAGATTGCTAAATCAACAGAAGATCCTTGGTGAGCAATATTAGAAGATAGAGGGGGGTAAACTGTTCATCCTGTTCCT GCACCATTTTCTACAATTCTTCTTGAAATTAATAAAGTTA,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/41-A_AP1_Co1R-araneta-arthropod.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/PXL_20241114_235255436-scaled.jpg,",19.8344244362,-155.1065979588,11/13/2024,"
$\"Photo$ Nothing out of the ordinary.<\/p>\n",Diptera,pcr,Whole arthropod,Edvotek Gel Electrophoresis,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Gel-2-bottom.jpg,","
I obtained my arthropod samples via an apple cider vinegar trap. I believe the flies I collected are fruit flies, but not necessarily a drosophilid. To prepare the DNA for PCR, both flies were ground in separate vials 100 \u00b5L of cell lysis buffer, and then another 100 \u00b5L was added. These vials were heated at 95oC for five minutes and after being cooled back down were vortexed and then centrifuged for two minutes at the highest speed. 150 \u00b5L of the supernatant from each tube was transferred into a new tube with 150 \u00b5L of prepared cold isopropanol. These were then vortexed and centrifuged for five minutes. As much supernatant as possible from each tube was transferred to a waste cup, and they were allowed to dry for a minute. 100 \u00b5L of 70% ethanol was added to each tube, they were vortexed and centrifuged for a minute, and then the supernatant was poured out and the tubes were allowed to dry for 10 minutes. 50 \u00b5L of DNA elution buffer was added to each tube, and they were incubated in a 65OC bath until the PCR reaction.<\/p>\n
The professor completed the PCR reaction, so my next tasks were to prepare the agarose gel. With a lab partner I prepared a double 6 well comb, by first adding 1 g of agarose gel to 50 mL of 1x TBE solution. We heated this in a microwave until the solution appeared clear, after cooling down to a safe amount we added 5 \u00b5L SYBR safe DNA dye and poured the solution in to the tray. After the gel set and the combes and rubber caps were removed, it was covered entirely in TBE buffer. I used parafilm to set up 5 drops of 2 \u00b5L 6X DNA loading dye. The first drop was mixed with a 10 \u00b5L of DNA ladder via a micropipette and loaded into the gel. The rest mixed with 10 \u00b5L DNA samples from my the PCR reactions of my arthropods. The actual electrophoresis and photography from the transilluminator were also done by the professor.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/D1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/D1-pt2.jpeg,",41.6760000000,-77.0678100000,08/31/2024,"
Location – Dirt on stream side top, new from flooding<\/span><\/p>\n
Notes – Light rain, many of same species around <\/span><\/p>\n",Staphylinidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/D1.png,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/C1.jpeg,",41.6760000000,-77.0678100000,08/31/2024,"
Location – Top of stream side, dirt new from flooding<\/span><\/p>\n
Notes – Many of same species around<\/span><\/p>\n",Nylanderia vividula,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/C1.png,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/A1.jpeg,",41.6760000000,-77.0678100000,08/31/2024,"
Location – Steep streamside dirt<\/span><\/p>\n
Notes – Sprinkling rain, multiple of same species observed <\/span><\/p>\n",Lasius,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/07/A1.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_2187.jpeg,",38.9420158044,-77.0891876176,09/16/2024,"
Caught in a pleasant park with many flowers ready for pollination. Caught during fall, with an outside temperature of 72 degrees Fahrenheit. Terrestrial habitat. Yellow and black, fuzzy, 6 legs, abdomen, 2 wings, disconnected head.<\/p>\n",Vespula squamosa,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Gel-1-wolbachia.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_2215.jpeg,","
DNA Extraction: It took a while to crush up the abdomen. There was a lot of debris. Had to add more Buffer ATL because some of it spilled while grinding. Forgot to change pipette tips between samples.<\/p>\n
PCR: Everything went according to the procedure. No errors made.<\/p>\n
Gel Electrophoresis, Arthropod CO1: Lane 1 had the DNA ladder. Lane 2 had the DNA from our first arthropod, arthropod B-1. Lane 3 had DNA from arthropod B-2. Lane 4 had DNA from arthropod B-3. Lane 5 had our positive control. Lane 6 had our negative control. Lane 7 had H2O. The controls worked.<\/p>\n
Gel Electrophoresis, Wolbachia: Lane 1 had the DNA ladder. Lane 2 had DNA from our first arthropod, artropod B-1. Lane 3 had DNA from arthropod B-2. Lane 4 had DNA from arthropod B-3. Lane 5 had our positive control. Lane 6 had our negative control. Lane 7 had H2O.<\/p>\n",No,ACTATTATTACTGCCCATGCATTTGTAATAATTTTCTTTATAGTTATGCCTTTCTTAATGGGAGGATTTGGAAATTGATT AATTCCTTTAATACTAGGTGTTCCAGATATAGCATTTCCTCGAATAAATAATATAAGATTCTGACTTTTACCCCCATCAT TATTCCTCTTAATTTTAAGAAATTTTATTGGAACAGGAGTAGGAACAGGATGAACTCTTTATCCTCCTCTTTCTTCTATT ACAGGTCATGATTCACCN,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/B-2-CO1F-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Arthropod-B-1-photo.png,",38.9419320802,-77.0891798938,09/05/2024,"
Caught near the front door of my house. Caught during fall. It was 79 degrees Fahrenheit outside when caught. Terrestrial\/urban habitat. Yellow and black stripes, 2 wings, abdomen, 6 legs, disconnected head.<\/p>\n",Apis mellifera,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Gel-1-wolbachia.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_2215-1.jpeg,","
DNA Extraction: It took a while to crush up the abdomen. There was a lot of debris. Had to add more Buffer ATL because some of it spilled while grinding. Forgot to change pipette tips between samples.<\/p>\n
PCR: Nothing went wrong, nothing to report.<\/p>\n
Gel Electrophoresis, Arthropod CO1: Lane 1 had the DNA ladder. Lane 2 had the DNA from our first arthropod, arthropod B-1. Lane 3 had DNA from arthropod B-2. Lane 4 had DNA from arthropod B-3. Lane 5 had our positive control. Lane 6 had our negative control. Lane 7 had H2O. The controls worked.<\/p>\n
Gel Electrophoresis, Wolbachia: Lane 1 had the DNA ladder. Lane 2 had DNA from our first arthropod, artropod B-1. Lane 3 had DNA from arthropod B-2. Lane 4 had DNA from arthropod B-3. Lane 5 had our positive control. Lane 6 had our negative control. Lane 7 had H2O. There was only 1 band present, in lane 5, which makes me think that something went wrong.<\/p>\n",No,TTATTATTCGAATAGAATTAAGATCCCCAGGATCATGAATTAGCAATGATCAAATTTATAATACAATTGTTACTAGTCAT GCATTCCTAATAATTTTTTTTATAGTTATACCATTTTTAATTGGAGGATTTGGAAATTGGCTTATTCCTTTAATACTAGG ATCACCTGATATAGCATTCCCCCGAATAAATAATATTAGATTTTGATTACTTCCTCCCTCATTATTTATACTTTTATTAA GAAATTTATTTTATCCAAGACCAGGAACTGGATGAACAGTATATCCACCATTATCAGCATATTTATATCATTCTTCACCT TCAGTAGATTTTGCAATTTTTTCTCTTCATATATCAGGAATTTCCTCAATTATAGGATCATTAAACTTAATAGTTACAAT TATAATAATAAAAAATTTTTCTATAAATTAT,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/B-1-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_1557.jpg,",43.5913396000,-70.3363805000,10/10/2024,"",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-21-151152.png,",41.7726307,-88.975774,,"
It was a warm and dry summer day of 90F. Caught in a tall grass area near Benedictine University’s parking garage.<\/p>\n",Polistes fuscatus,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG-20241015-WA0002.jpg,","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Wasp-JV-DNA.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0297.jpeg,",38.9785757955,-77.0907119965,09/04/2024,"
Caught in my garage, urban environment. Early fall, 74\u00baF. Grey and brown with longs legs including two large hind legs. Rounded body with hard shell. Pointed head with small mandibles and long vertical eyes. Long thin antennae.<\/p>\n",Orthoptera,DNeasy (Qiagen),Partial abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Gel-Electrophoresis-4-5.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0295.jpeg,","
DNA Extraction: We extracted DNA from the bug matter within the abdomen of the specimen.<\/p>\n
PCR: Everything when according to protocol.<\/p>\n
Gel Electrophoresis, Arthropod CO1: The first lane contains the DNA ladder. The second contains the sample from the Camel Cricket (C-4). The third lane has DNA from another specimen. The fourth and fifth lanes contain positive and negative controls and the sixth lane was our water control.<\/p>\n
Gel Electrophoresis,\u00a0Wolbachia<\/em>:<\/p>\n
The first lane contains the DNA ladder. The second contains the sample from the Camel Cricket (C-4). The third lane has DNA from another specimen. The fourth and fifth lanes contain positive and negative controls and the sixth lane was our water control. The ninth lane on the far right has DNA from another specimen.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0299.jpeg,",38.9785058102,-77.0909660499,09/10/2024,"
Caught under a door leading outdoors, urban environment. Early Fall, 70\u00baF. Large black hairy legs and
\nantennae.<\/p>\n",Blattodea,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0300.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0293.jpeg,","
DNA Extraction: We extracted DNA from the bug matter within the abdomen of the specimen.<\/p>\n
PCR: We followed to protocol and didn\u2019t notice any mistakes.<\/p>\n
Gel Electrophoresis, Arthropod CO1: The first lane contains the DNA ladder. The second and third lanes have DNA from two other specimens. The fourth lane has the DNA from the cockroach (C-3). The fifth and sixth lanes contain positive and negative controls and the seventh lane was our water control.<\/p>\n
Gel Electrophoresis,\u00a0Wolbachia<\/em>:<\/p>\n
The first lane contains the DNA ladder. The second and third lanes contain DNA from two other specimens. The Forth lane has DNA from another specimen. The fourth lane contains DNA from the cockroach (C-3). The fifth and sixth lanes contain positive and negative controls and the seventh lane was our water control.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Wasp.jpg,",41.77564,-88.0970,09/24/2024,"
It was a dry and hot day. Collected from the marshy area by the garage.<\/p>\n",Hymenoptera,tweezers,Abdomen,MiniPCR,Other,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/gelelec-1.jpeg,","",Yes,GCTAGAACAGGTAAAGATAATAAAAGTAAAATTGCAGTAATTAAAACTGATCATGTAAATATAGGTAAAAAATTTAATGA ATGAGTTTTAATGTGTATATTTAAGATTGTTACAATGAAATTAATAGCTCCTATGATTGATGAAATTCCTGCAATGTGAA GAGAGAAAATAGCAAAATCAACTGATGATGAATTGTGACCTATATTAGATGATAAAGGTGGATATAAAGTTCATCCAGTT CCTACTCCTATATCATTAATATTTCTAATAATTAATAATAATAATGATGGTGGTAATAATCAAAATCTTATATTATTTAT TCGTGGGAATGCTATATCTGGGGCACCTAGTATTAAGGGAATTAATCAATTTCCAAATCCTCCAATTATAAATGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/2b073b6a-554e-4b7e-8af0-1270a0d6725b-2.jpg,",41.794402,-88.08030509999999,09/03/2024,"
IT WAS A DRY WARM DAY. WE COLLECTED BY THE POND NEAR BENEDICTINE UNIVERSITY PARKING GARAGE<\/p>\n",Lepidoptera,Qiagen Blood and Tissue kit,Abdomen,MiniPCR,Other,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/c3ed785f-5c84-428d-946f-e76e27e5c266.jpg,","",No,>47-A_AG1_Co1R (1).scf (380 bp) AAAACAGGTAAAGATAAGAGTAACAATAAAGCTGTAATTCCTACAGATCAAACAAATAAAGGTATTTGGTCAAATGATAA ATTTTTAATTCGTATATTAATGATTGTTGTAATAAAATTAATAGCTCCTAAAATTGAAGAGATTCCAGCTAAATGAAGAG AAAAAATTGCTAAATCAACAGAAGATCCTTGATGAGCAATATTAGAAGATAAAGGGGGGTAAACTGTTCAACCTGTTCCT GCACCATTTTCTACAATTCTTCTTGAAATTAATAATATTAATGAAGGAGGTAATATTCAAAAACTTATATTATTTATTCG AGGGAAAGCTATATCAGGAGCTCCTAGTATTAAAGGTACTAATCAATTTCCAAATCCTCC,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/47-A_AG1_Co1R-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/TL_housefly_1-1-rotated.jpg,",45.515232,-122.6783853,10/15/2024,"",Musca domestica,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Tuesday-Gels-2024.pptx-5.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-25-162529.png,",45.51249800000001,-122.686763,10/17/2024,"",Aphididae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/20241113_170421-scaled.jpg,","",No,,,, "",45.515232,-122.6783853,10/15/2024,"",Lycosidae,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Tuesday-Gels-2024.pptx-4.jpg,","",No,,,, "",45.5152320000,-122.6783853000,10/15/2024,"",Araneus diadematus,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Tuesday-Gels-2024.pptx-3.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-25-160402.png,",45.5469616000,-123.1386021000,10/16/2024,"",Tapinoma sessile,DNeasy (Qiagen) blood and tissue kit,,Standard electrophoresis system,TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Copy-of-Thursday-Gels-2024.pptx-3.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-25-155840.png,",45.515232,-122.6783853,10/15/2024,"",Pieris rapae,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Tuesday-Gels-2024.pptx-2.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-25-154512-1.png,",45.515232,-122.6783853,10/15/2024,"",Formicidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Tuesday-Gels-2024.pptx-1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-25-153823.png,",45.5130742000,-122.6880142000,10/06/2024,"",Leptoglossus occidentalis,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Copy-of-Thursday-Gels-2024.pptx-2.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-25-153115.png,",45.5411226,-122.6600478,10/14/2024,"",Lithobius forficatus,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Tuesday-Gels-2024.pptx.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-25-151341.png,",45.5469616000,-123.1386021000,10/13/2024,"",Polistes dominula,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Copy-of-Thursday-Gels-2024.pptx-1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/unnamed-3.jpg,",19.7394516432,-156.0120801517,11/21/2024,"
Small black ants provided by instructor; must have been easy to catch.<\/p>\n",Arthropoda,pcr,Whole arthropod,Standard electrophoresis system,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/unnamed-1-1.jpg,","
Gel Lanes (Top)<\/strong><\/p>\n
\n
DNA ladder<\/li>\n
Sample 1 CO1<\/li>\n
Sample 2 CO1<\/li>\n
Sample 1 16S<\/li>\n
Sample 2 16S<\/li>\n<\/ol>\n
Experimental Set up<\/strong><\/p>\n
The collection of two insect specimens were subjected to DNA extraction for analysis under the process of cell lysis, DNA precipitation, and DNA elution. Specimens were rinsed with water to remove excess ethanol, and then transferred into two separate 1.5 mL microcentrifuge tubes. A sterile pestle grinds the sample after a 200 \u00b5L Cell Lysis Buffer was added, ensuring optimal maceration. To rid of debris, the samples were heated to 95\u00b0C and incubated approx. 5 minutes and centrifuged for 2 minutes. The results contained a supernatant that is then precipitated using cold isopropanol (150 \u00b5L) and centrifuged for 5 minutes. Products are air-dried and rid of supernatants whilst ensuring that the DNA pellets are left behind. Pellets are then washed with 100 \u00b5L of 70% ethanol. 50 \u00b5L of DNA Elution Buffers are added to each tube and incubated in a 65\u00b0C water bath for approx. 5 minutes. Once incubated, 2 \u00b5L of the DNA extracted are extracted to pre-aliquoted PCR mixes for the appropriate arthropod CO1 and wolbachia 16S rRNA gene. The PCR tubes are then amplified in a thermal cycler using appropriate steps with optimal temperature DNA amplification. To prepare for visualizing the extraction of the potential DNA and bacteria, a 2% agarose gel would be created by dissolving 0.5g of agarose powder in 25mL of a 1XTBE buffer, cooled, and then poured into a tray. Samples would be mixed with heavy loading die, loaded into the vents, and would undergo electrophoresis on a 2% agarose gel mixed with SYBR Safe DNA dye. The test would be run at 130V for ~21 minutes. Bands were visualized in a transilluminator and photographed for analysis. <\/span><\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/20241114_134415-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/20241205_100629-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/20241205_100640-scaled.jpg,",19.7031566000,-155.2382164000,11/14/2024,"
Approximately 1 cm in length. Brown\/orange limbs with darker brown\/black head, wings, and abdomen. Is able to fly. Attracted to lights.<\/p>\n",Ananca bicolor,pcr,Abdomen,Standard electrophoresis system,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/wolbachia-final-2.png,","
2% agarose gel with a 1kb DNA ladder loaded into lane 1<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG-20241117-WA0002.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG-20241117-WA0001.jpg,",19.6615305197,-155.9804959833,11/13/2024,"
Easy to catch. Plump, shiny, black fly. Lucilia Sericata species suspected.<\/p>\n",Arthropoda,pcr,Whole arthropod,Edvotek Gel Electrophoresis,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/WhatsApp-Image-2024-11-24-at-11.00.25_dcf838a5.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/WhatsApp-Image-2024-11-24-at-11.00.25_397f4fa7.jpg,","
Gel Lanes (Bottom of gel 1)<\/strong><\/p>\n
\n
DNA ladder<\/li>\n
Sample 1 CO1<\/li>\n
Sample 2 CO1<\/li>\n
Sample 1 16S<\/li>\n
Sample 2 16S<\/li>\n<\/ol>\n
Gel 2 is the control PCRs. Lanes (Top with CO1, Bottom with 16S primers)<\/p>\n
\n
DNA ladder<\/li>\n
Wolbachia + DNA<\/li>\n
Wolbachia – DNA<\/li>\n
Pre-extracted Wolbachia DNA<\/li>\n
Water only<\/li>\n<\/ol>\n
<\/p>\n
Materials and Methods<\/strong><\/p>\n
DNA was extracted from two insect specimens using cell lysis and precipitation protocol. Specimens were rinsed in water to remove ethanol, blotted dry, and transferred to individual 1.5 mL microcentrifuge tubes. Each sample was macerated in 200 \u00b5L of Cell Lysis Buffer using sterile pestles, with thorough grinding to maximize DNA yield. The tubes were heated at 95\u00b0C for 5 minutes, and centrifuged to remove debris. The supernatant was transferred to tubes containing cold isopropanol, mixed, and centrifuged to pellet DNA. Pellets were washed with 70% ethanol, air-dried, and resuspended in 50 \u00b5L DNA Elution Buffer and incubated in a 65\u00b0C\u00a0 water bath for approximately 5 minutes. The pellet was still intact at this stage, but it was broken up by pipetting up and down a few times. It was left to incubate for an additional minute before proceeding.<\/p>\n
For PCR amplification, 2 \u00b5L of each DNA extract was added to a pre-mixed PCR master mix containing primers for arthropod CO1 and Wolbachia<\/em> 16S rRNA genes. Amplification was conducted using a thermal cycler with appropriate annealing temperatures for each gene. PCR products were visualized via electrophoresis using a 2% agarose gel stained with SYBR Safe DNA dye. Gels were prepared by dissolving 0.5 g agarose in 25 mL 1\u00d7 TBE buffer, cooled, and poured into a casting tray. Samples were mixed with loading dye, loaded into wells, and electrophoresed at 130 V for ~25 minutes. Bands were visualized under UV light, photographed, and analyzed.<\/p>\n",No,``,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_0841.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_0840.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_0839.jpeg,",38.9423307062,-77.0621076787,09/17/2024,"
Collected Outside, 70\u00baF, wet ground, only one of its kind in the area. Terrestrial, urban habitiat. Yellow, spotted, small.<\/p>\n
<\/p>\n",Diabrotica undecimpunctata,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0850.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0848-1.png,","
Crushed, extracted abdomen, no exoskeleton. PCR used to amplify the specific genes. Dyed the samples of DNA, mixed, and put into respective gel spots.<\/p>\n
<\/p>\n
Arthropod CO1: No mistakes, controls worked to perfection.<\/p>\n
Gel electrophoresis: No mistakes, controls worked.<\/p>\n
1-kb ladder<\/p>\n
2- C-4<\/p>\n
3- C-5<\/p>\n
4- W+<\/p>\n
5- W-<\/p>\n
6- H2O<\/p>\n
7 – empty<\/p>\n
8- empty<\/p>\n
9 – rerun of C-3<\/p>\n
PCR: No mistake<\/p>\n",No,Diabrotica undecimpunctata,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Copy-of-C-5-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/k-scaled.jpg,",32.3677213363,-86.1810161963,08/29/2024,"
A tiny insect with a clear body and red spots along its sides. It appears to be some type of bristletail.<\/p>\n",,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TBE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Wolbachia-Kris-Dylan-Exp.-0.900-secEthidium-Bromide.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Arthro-Kris-DylanEthidium-Bromide.jpg,","",Unknown,,,, "",32.3706320080,-86.1792302900,08/29/2024,"
A very microscopic mite colored a reddish orange with long two front legs located on the front and back of it.<\/p>\n
Was not able to get a picture due to the microscope preventing a photo from properly being taken.<\/p>\n",Arachnida,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TBE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Arthro-Kris-DylanEthidium-Bromide.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Wolbachia-Kris-Dylan-Exp.-0.900-secEthidium-Bromide.jpg,","",No,TnnCnnCnnGnnnnnnnnnAnnnGGGnnnGCnnTnnnnnnnnnTnTTCnnnnnGnnnnnnnnnnACnnnATCTTnnnnCnnAnAnnAnnnnnTTTACnnnnnnnnnnTnACnGCnnnnnnnnTTATCAnnnnTTTCnTTATGGnnnnnnnnGCnnTnnnnnnnnnTnnnnnnnAnnnnnnTGnnnnnnnnAnnnnnnnnnnnnnnGATATnnnnnnCnnnCnnAnAAATAAnAnnnnnnTCnGATTACTACCCCCCTCTCnTAnACTTCTATTAnnCnnnnnnnTnnTCnnnnnnnnnnnnnGnnnnGGATGAAnTGnCnAnnnnnnnnnnnnnnGTnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnTTTnnnnnnnnnnnnnnnnnnGTAnnnnnnnnnnnnnnnnnnnnnnnTTTnnnnnnnnnnATnnnnnnTAnnnnAnnnnnnnnnnnnnnnnnnnnnnNnnnnCnnnnnnnnnnnnnnnnnNnnnnnnnnnnNnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnNNnnnnNNNNNnnG,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/KDA9-CO1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/20240905_125353-scaled.jpg,",32.3709263042,-86.1791081119,,"
Ostracod with round body, yellow coloration with some darker brown areas.<\/p>\n",Podocopida,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TBE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Wolbachia-Kris-Dylan-Exp.-0.900-secEthidium-Bromide.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Arthro-Kris-DylanEthidium-Bromide.jpg,","",No,TnnNNNnnnnnnnnnnnnnnnTnnnnnnnnnnnnnnnnnnnnnnnTCnAnnnnAnTTnnnnnnnnnnnnnnnnnnnnnnn nNnnnGATCnnnnTnnnnnnnnnnnnnnnnnCnnnnnnTGCTTTTATTATAATTTTTTTTATAGTTATnCnnnnnATAAT TnnnnGATTTnnnnnnnnnnnnnTTCnnnnnnnnnnnnnAnnnnCTGATATAnCnnnnnnnnnnnnnAATAAnATAnnnn TnnnnnnnnnnCnnCCnnnnnnnnnnnTAnTnnnnnnnnnnnnnnnnnnnnnnnnnnGnnnnnGAACnnnnnnAAnnnnn nnnCCCCnnnnnnnnTCnAAnnnnnnnnnnnnnGGnnnnnnnnnnnnnnnnnnnnnnnnTnnnnnnnnnnnnnnnnGnnn nnnnnnnnTTnnnnnAnCnnnnnnnTTnnnnnnnnCnnTnATnAATATnnnnnnnnnNNNnnnnnnnnTnnAnnnnnnnn nnnnnnnnnnnnnAnnnnnnnnnnnTACAnnnnnnnnnNnnnnnnnnnnnnnnCnnGnnnnnnCnnGNnCTATTACnnTn nTnnnAACnnnnnnAAnTnTnnnnnnnnnnnTnnnnnnnnnnnnnnnnnnnnnAnnnnCnnnnnnnnnnnnnnnnTnnnT TnnnnTTTT,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/KDA8-CO1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/20240829_121445-scaled.jpg,",32.3677948314,-86.1807378177,08/29/2024,"
A black carpenter ant found roaming in leaf litter.<\/p>\n",Formicidae,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Arthro-Kris-DylanEthidium-Bromide.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Wolbachia-Kris-Dylan-Exp.-0.900-secEthidium-Bromide.jpg,","",Yes,TTGnNnnTTnnnCGGGnnnAATTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTAGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCAATACnATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTAATTCCACTTATACTAGGATCTCCTGATATAGCTTACCCTCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGAACTATCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATnnTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACAATTATAAATATACATAATTCnnnTATTTCCCTAGATAAAATTCCCTTATTAGTATGATCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAnGnGCTATTACAATACTACTAACAGACCGAAATCTTAATACTTnnTTnnTnnnnCnCTCnGGnnGnnGnnnnnnnAnTTnnTnCnnAnnTTTnnTTnnnnTTTT,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/KDA7-CO1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/20240905_121914-scaled.jpg,",32.3707164219,-86.1789628460,08/29/2024,"",Odonata,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TBE,Ethidium Bromide,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Wolbachia-Kris-Dylan-Exp.-0.900-secEthidium-Bromide.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Arthro-Kris-DylanEthidium-Bromide.jpg,","",No,TGGGnnGGnNnAATTGGTACGGCACTAAGAGTTTTAATTCGAATTGAATTGGGTCAACCCGGATCCCTAATTGGCGATGA TCAAATTTATAATGTGATTGTAACTGCACATGCATTTGTTATAATTTTCTTCATAGTAATACCTATTATAATTGGGGGTT TCGGAAACTGATTAGTACCCCTAATATTAGGAGCGCCAGATATGGCCTTCCCCCGATTGAATAACATAAGATTCTGACTT TTACCCCCCTCTTTCACATTACTTTTAGCAAGTAGAATGGTTGAAAGAGGGGCAGGAACAGGTTGAACTGTATACCCTCC TTTAGCTGGGGCAATTGCACATGCTGGAGCCTCAGTAGATTTAACCATTTTTTCTCTACATTTGGCTGGGGTATCATCAA TTCTAGGAGCGATTAATTTTATTACAACCGTAATCAATATGAAGTCACCAGGAATGAAGTTAGATCAGCTGCCATTATTT GTATGAGCAGTAGTAATTACTGCAGTATTGTTATTATTATCACTTCCTGTATTAGCTGGGGCTATTACAATGTTGTTAAC TGATCGAAATATTAATACATCCTTCTTTGATCCnGCAnGAGGGGGGGnnCCAATTCTTTACnnACACCTCTTCnnAnTTT T,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/KDA4-CO1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/tempImageXCcubq-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/tempImage38ptd9-scaled.jpg,",33.9898201110,-84.4397187000,11/12/2024,"
Found on a web at the top of a tree. Tree in a parking lot.<\/p>\n",Trichonephila clavata,DNeasy (Qiagen),Partial abdomen,MiniPCR,TBE,SYBR Safe,"","",Yes,>7_Emily_spider-CO1F ACTGCAATAAGAGTATTAATTCGTACTGAGCTTGGACAGCCAGGAAGATTTATAGGGGATGATCAATTATATAATGTAAT TGTTACAGCTCATGCATTTGTGATAATTTTTTTTATAGTAATACCAATTTTAATTGGTGGATTTGGAAATTGATTGGTTC CTTTAATATTAGGAGCGCCAGATATAGCGTTTCCTCGAATAAATAATTTAAGGTTTTGACTATTGCCTCCTTCTTTATTT TTATTGTTCGTTTCTTCAATAGTAGAGATAGGAGTGGGAGCAGGTTGGACAGTTTACCCTCCTTTATCATCTTTAGATGG ACATTCTGGAAGGTCAGTGGATTTTGCTATTTTTTCTTTACATTTAGCTGGGGCTTCTTCAATTATAGGGGCTATTAATT TCATTTCTACTATTGTGAATATACGATCTTTTGGAATAACTATAGAAAAGGTACCTTTATTTGTTTGGTCTGTTTTAATT ACTGCTGTTTTATTACTTTTATCTTTACCTGTATTAGCAGGTGCTATTACAATATTATTGACTGATCGAAATTTTAATAC ATCATTTTTTGACCCTTCTGGAGGGGGGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/tempImageikJb63-scaled.jpg,",33.9886738530,-84.4381681600,11/12/2024,"
Found on a dead tree log on the ground. Crawling on top of the log.<\/p>\n",Solenopsis invicta,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,SYBR Safe,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_1583_page-0001-scaled.jpg,",33.9904904000,-84.4395146000,11/12/2024,"
In moist soil, found under a bed of leaves in a foresty area.<\/p>\n",Camponotus pennsylvanicus,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6942-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6950.jpeg,","
Sample is in lane 2 in both of the gels<\/p>\n",No,GGAACAGGAACAGGATGGACTATTTATCCTCCCTTATCCTCTAATATTTTTCATAGTGGTCCTTCTGTAGACTTAACAATTTTTTCTCTTCATATTGCAGGTATATCCTCAATTTTAGGAGCAATTAATTTTATTTCAACAATTCTTAATATACATCATAAAAATTTTTCTATTGATAAAATTCCTTTACTCGTATGATCAATTTTAATTACAGCTATCTTACTTCTATTATCCTTACCTGTATTGGCCGGAGCTATTACTATACTATTAACTGATCGAAATTTAAATACTTCATTCTTTGATCCTTCGGGAGGAGGCG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_1595-1-scaled.jpg,",33.9904904000,-84.4395146000,11/12/2024,"
Found in moist soil near a creek in a forest.<\/p>\n",Ptyoiulus impressus,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6942-2.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_6950-2.jpeg,","
Both of the samples are in lane 3 of the gels.<\/p>\n",Unknown,GAACAGGTTGAACAGTCTATCCCCCCTTAGCTAGTAACGTATCACACTCCGGAGCTTCAGTAGACATAGCAATTTTTTCACTTCATCTAGCAGGGGCCTCCTCAATTCTAGGAGCCATTAATTTTATTACCACAATTATTAATATACGAAGAGCAGGTATGTTGTTTGAACAAATACCCTTATTTGTGTGGGCAGTAAAAATTACAGCCATTTTACTTCTTTTATCTCTCCCCGTTTTAGCTGGTGCTATTACTATGCTCTTAACAGATCGGAACTTTAACACTTCTTTTTTCGACCCTGCAGGGGGAGGAGACC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_2949-scaled.jpeg,",33.9883549374,-84.4386374891,11/12/2024,"
Found in forest environment, rapid movement, moist ground under leaves<\/p>\n",Hymenoptera,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4309-scaled.jpeg,","
There were bands that appeared but the results were not conclusive due to some experimental error during PCR, so no data was sent for sequencing<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_5913-scaled.jpeg,",33.98827242053473,-84.43881417828337,11/12/2024,"
Found under rock in wet forest environment, slow moving<\/p>\n",,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_4309-1-scaled.jpeg,","
During PCR there was significant experimental error, so the bands that appeared were not conclusive and DNA was not sent for sequencing.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-20-at-3.45.49 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-20-at-3.45.55 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-20-at-3.46.04 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-20-at-3.46.09 PM.png,",33.9892669000,-84.4404690000,11/12/2024,"
Highly active and mobile; surrounded by other species of millipede; flat segments of nearly uniform brown plates (with some flecks of darker brown and white) are overlain to create level appearance; legs are translucent in appearance<\/p>\n",Polydesmus angustus,DNeasy (Qiagen),Body without legs,MiniPCR,TBE,GelGreen,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-20-at-3.30.30 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-20-at-3.30.42 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-20-at-3.30.50 PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-20-at-3.30.58 PM.png,",33.9892669000,-84.4404690000,11/12/2024,"
Dark Brown with distinct orange, red stripes between armored plate segments; 2 legs per plated body segment; flat-backed appearance created by overlaying plates.<\/p>\n
Somewhat active\/mobile; larger specimen indicative of more maturation.<\/p>\n",Euryurus leachii,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_4464-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_4393-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_4386-scaled.jpeg,",33.9892669000,-84.4404690000,11/12/2024,"
-moves frequently, explores test tube<\/p>\n
-pattern and various colors present on back, mix of light and dark browns<\/p>\n
-lots of light colored legs<\/p>\n
-segmented antennae<\/p>\n",Delophon georgianum,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-27-at-10.20.08 AM.png,","
The control groups run in the gel electrophoresis were successful, but the band was not visible. The sample was not sent for sequencing.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_4457-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_4400-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_4397-scaled.jpeg,",33.9904904,-84.4395146,11/12/2024,"
-attempt to hide under pieces of soil in collection tube<\/p>\n
-segmented body, abdomen darker in color with some pattern and spotting<\/p>\n
-lighter colored, segmented legs<\/p>\n",Steatoda triangulosa,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/Screenshot-2025-01-27-at-10.20.08 AM.png,","
The controls for Wolbachia\u00a0<\/em>were successful when the gel was run, but there was no visible band. The sample was not sent for sequencing.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_3366-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_3365-scaled.jpeg,",38.9423845497,-77.0620848611,05/29/2024,"
Location: Found in alley
\nSeason: Spring
\nTemperature: 76\u00b0
\nHabitat: Urban<\/p>\n
Description: Part of right wing is gone\/has injured wing. Light green wings with two spots on each wing. Tan antennae, white torso, fuzzy texture.<\/p>\n",Actias luna,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_2220-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_2221.jpeg,","
PCR: No mistakes made during PCR<\/p>\n
Gel electrophoresis, Arthropod CO1:
\nLane 1: Ladder
\nLane 2: Arthropod A1 DNA
\nLane 3: Arthropod A2 DNA
\nLane 4: Arthropod A3 DNA
\nLane 5: Positive Wolbachia Control
\nLane 6: Negative Wolbachia Control
\nLane 7: Water<\/p>\n
No errors during the lab; our controls worked as intended<\/p>\n
Gel Electrophoresis, Wolbachia:
\nLane 1: Ladder
\nLane 2: Arthropod A1 DNA
\nLane 3: Arthropod A2 DNA
\nLane 4: Arthropod A3 DNA
\nLane 5: Positive Wolbachia Control
\nLane 6: Negative Wolbachia Control
\nLane 7: Water<\/p>\n
No errors during the lab; our controls worked as intended<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_3397-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_3367-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_3400-scaled.jpeg,",38.9609911297,-77.0464970138,09/07/2024,"
Location description: Found on sidewalk
\nSeason: Summer
\nTemperature: 60\u00b0
\nHabitat: Urban<\/p>\n
Color: yellow, orange, dark red<\/p>\n
Six legs, two wings, orange antennae, abdomen tapers to a point<\/p>\n
Mandibles: dark red<\/p>\n",Vespa crabro,DNeasy (Qiagen),Partial abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_2220-3.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_2221-3.jpeg,","
Gel Electrophoresis, Arthropod CO1 (Round 1):
\nLane 1: DNA Ladder
\nLane 2-4: Arthropod DNA (A1-A3)
\nLane 5: Positive Control
\nLane 6: Negative Control
\nLane 7: Water<\/p>\n
Gel Electrophoresis, Arthropod CO1 (Round 2):
\nLane 1: DNA Ladder
\nLane 2-4: Arthropod DNA (A4-A6)
\nLane 5: Positive Control
\nLane 6: Negative Control
\nLane 7: Water<\/p>\n
Gel Electrophoresis, Wolbachia 16s (Round 1):
\nLane 1: DNA Ladder
\nLane 2-4: Wolbachia DNA (A1-A3)
\nLane 5: Positive Control
\nLane 6: Negative Control
\nLane 7: Water<\/p>\n
Gel Electrophoresis, Wolbachia 16s (Round 2):
\nLane 1: DNA Ladder
\nLane 2-4: Wolbachia DNA (A4-A6)
\nLane 5: Positive Control
\nLane 6: Negative Control
\nLane 7: Water<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Arthropod-A-2.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0680.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0679.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0678.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0677.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0676.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0675.jpeg,",38.9516143000,-77.1197521000,09/28/2024,"
caught in a public park, temperature of about 70 degrees Fahrenheit, terrestrial habitat.<\/p>\n
<\/p>\n
Two big grey wings, two antennae, appears to be a moth.<\/p>\n",Atalopedes campestris,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Image-11-14-24-at-2.48 PM-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Image-11-14-24-at-2.48 PM.png,","
DNA Extraction:<\/p>\n
I made sure to crush the arthropod very well, but there were large chunks of exoskeleton that were difficult to break up. As a result, the sample had to be spun 1-2 extra times in the centrifuge for the steps using the filter.<\/p>\n
<\/p>\n
Gel Electrophoresis, arthropod CO1:<\/p>\n
1 – DNA ladder<\/p>\n
2 – A4 DNA<\/p>\n
3 – A5 DNA<\/p>\n
4- A6 DNA<\/p>\n
5 – Arthropod positive control<\/p>\n
6 – Arthropod negative control<\/p>\n
7 –\u00a0 water<\/p>\n
Analysis: all controls showed expected bands, and the sample showed an arthropod CO1 band.<\/p>\n
Gel electrophoresis, Wolbachia:<\/p>\n
1 – DNA ladder<\/p>\n
2 – A4 DNA<\/p>\n
3 – A5 DNA (lascoria ambigualis)<\/p>\n
4- A6 DNA<\/p>\n
5 – Wolbachia positive control<\/p>\n
6\u00a0– wolbachia negative control<\/p>\n
7 –\u00a0 water<\/p>\n
Analysis: all controls showed expected bands, and the sample did not show a wolbachia band.<\/p>\n
<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/thumbnail_image6.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/thumbnail_image5.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/thumbnail_image4.jpg,",33.9892669000,-84.4404690000,11/12/2024,"
The arthropod is small, long, and appears a beige yellow. Compared to the rest of the body, the head is slightly darker (burnt sierra color). It has many legs and a long body shape. It is segmented but does not have a thorax or an abdomen (indicating that it is not an insect). Its antennae are long and segmented. It is likely in its early stages of development (juvenile\/nymph form); it is a miniature version of the adult, but shorter (about an inch long while the adults can get up to 4 inches).<\/p>\n",Geophilus flavus,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3244-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3245-2-scaled.jpg,","
No visible bands appeared in the prospective centipede wells for both the Wolbachia<\/em> and the arthropod gel electrophoresis. Since it is not evident that there is enough genetic material to analyze, the results are inconclusive, so this sample was not sent in for sequencing.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/thumbnail_image2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/thumbnail_image3.jpg,",33.9892669000,-84.4404690000,11/12/2024,"
The arthropod is long and has many, many legs (over a hundred pairs) with two pairs per segment. It is of a dark brown color with a segmented exoskeleton. The underbelly is a cream color, distinguishing it from many other millipede species. It has two visible eyes and antennae that are bent. The body is segmented, but it does not have a thorax or an abdomen; therefore, it is not an insect. It is about an inch and a half long.<\/p>\n",Ptyoiulus impressus,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3244-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/IMG_3245-1-scaled.jpg,","
No visible bands appeared in the prospective millipede wells for both the Wolbachia<\/em> and the arthropod gel electrophoresis. Since it is not evident that there is enough genetic material to analyze, the results are inconclusive, so this sample was not sent in for sequencing.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/blackCleaver-scaled.jpg,",33.9892669000,-84.4404690000,11/14/2024,"
It is black all-round the body with large mandibles.<\/p>\n",Camponotus pennsylvanicus,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/3-arth-1.jpeg,","
Lane 12<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/ant-1-scaled.jpg,",33.9888650241,-84.4397611715,11/14/2024,"
Brownish in color, with a tint of red on the head. The abdomen has some black as well.<\/p>\n",Lasius,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/3-arth-1.jpeg,","
Lane 11<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3359-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3358-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3357-scaled.jpg,",33.9876415546,-84.4395146180,11/12/2024,"
Spider found in a web about 4 feet off the ground. First attempted capture failed and spider moved away, but second attempt succeeded in capture. Spider formed a web in container before it was killed. Has eight furry legs and a relatively small body.<\/p>\n",Barronopsis texana,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/3-arth.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/3-wol.jpeg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3356-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3355-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3353-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3354-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3352-scaled.jpg,",33.9880417229,-84.4391809838,11/12/2024,"
3 centimeter long millipede. Has 2 layers of legs on each side of the segmented body, and has one break in the middle, likely due to human injury. Found under a rock curled up, but expanded and began exploring once put into container.<\/p>\n",Julidae,DNeasy (Qiagen),Partial abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/3-wol.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/3-arth.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/D11FCE3C-282B-4D5C-B001-4C947C09D401-scaled.jpeg,",33.9877621518,-84.4406514108,11/12/2024,"
Found from a water sample; vibrant yellow in color; immobile after treatment; slight color variations manifested within main body; circular in shape; and likely in larval stage (minuscule size indicates early in development).<\/p>\n",Pisidium moitessierianum,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/5-wol-1.jpeg,","
\n
Low quality DNA score likely led to contamination that resulted in lack of effective sequencing<\/li>\n
Could not find a match in BLAST<\/li>\n
Was not present in class cladogram (still yet to find reason)<\/li>\n<\/ul>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/30F72F9F-7E43-4F33-93AD-4DC28EBEB721-scaled.jpeg,",33.9876276290,-84.4404168464,11/12/2024,"
Stinkbug image; found climbing up a tree branch; engaged in swift motion; grey in color; presence of small, minuscule eyes; and symmetric over the horizontal axis.<\/p>\n",Issidae,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/5-wol.jpeg,","",No,,,, "",36.0784153552,-86.7759563320,,"",Coleoptera,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0525.jpeg,",42.1034075,-76.2621549,10/11/2024,"
<\/p><\/blockquote>\n",Arthropoda,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0504.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0503.jpeg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/1000009547.jpg,",33.9904904000,-84.4395146000,11/13/2024,"
\n
Was located under dirt and actively moving around, occasionally going under the leaves and dirt. Has a body bigger than the average black carpenter ant with a large abdomen.<\/li>\n<\/ul>\n",Hymenoptera,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/1000011807-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/1000011806-scaled.jpg,","
Gel Image: Lane 9 and 10<\/p>\n",Yes,TATTTGATTTTTGGAGCTTGAGCTGCTATAGTAGGTACAGCAATAAGAGTATTAATCCGTATAGAATTGGGTCAAACTGGAAATTTTATAGGTGATGATCATTTATATAATGTAATTGTAACTGCTCATGCTTTTGTAATGATTTTTTTTATAGTAATGCCTATTATAATTGGAGGTTTTGGTAATTGATTAGTTCCTTTAATGTTGGGGGCTCCAGATATAGCTTTTCCCCGAATAAATAATTTGAGTTTTTGATTATTACCCCCTTCTCTAATATTATTATTTATTTCTTCCATAGCAGAAATAGGGGTTGGAGCTGGATGAACAGTATATCCACCTTTGGCATCCATTGTAGGTCATAATGGAAGCTCAGTAGATTTTGCCATTTTTTCTTTACATTTGGCGGGTGCTTCATCTATTATAGGGGCTATTAATTTTATTTCTACTGTTATTAATATACGGTCAATTGGAATATTCTTAGACAAGGTACCTTTGTTTGTATGATCTGTAGTTATTACGGCCGTATTATTACTTCTTTCATTACCGGTATTGGCTGGTGCTATTACTATATTATTGACTGATCGTAATTTTAATACTTCTTTTTTTGATCCTGCAGGAGGGGGGGACCCTATTTTATTTCAACATTTGTTTTGATTTTTGTCCCCCTNNAAAAAATTTAAAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/1000009543-1.jpg,",33.9837691000,-84.4423179000,11/14/2024,"
<\/p>\n
\n
The moth fly was mainly stationary and resting on a wall and when captured, flew around a while before resting again on wall of container again.<\/p>\n",Psychodidae,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/1000011807-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/1000011806-1-scaled.jpg,","
Gel image: Lane 9 and Lane 10<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_8170-scaled.jpeg,",33.9892669000,-84.4404690000,11/12/2024,"
Found on a spider web <\/span>between two trees in the Walton outdoor classroom space.<\/span><\/span>\u00a0<\/span><\/p>\n
\n
Temp: 12.1<\/span>\u00b0<\/span>C<\/span>\u00a0<\/span><\/li>\n<\/ul>\n
\n
Longitude: 33<\/span>\u00b059\u201918\u2019\u2019N<\/span>\u00a0<\/span><\/li>\n<\/ul>\n
\n
Latitude: 84\u00b026\u201919\u2019\u2019W<\/span><\/li>\n<\/ul>\n",Trichonephila clavata,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/4-arth.jpeg,","
The DNA PCR was not successful because the bands did not correlate to the Wolbachia control band.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_8175-scaled.jpeg,",33.9892669000,-84.4404690000,11/12/2024,"
\n
Found underneath a log in the dirt.<\/li>\n
Temp: 12.1<\/span>\u00b0<\/span>C<\/span>\u00a0<\/span><\/li>\n<\/ul>\n
\n
Longitude: 33<\/span>\u00b0N<\/span>\u00a0<\/span><\/li>\n<\/ul>\n
\n
Latitude: 84\u00b0W<\/span><\/li>\n<\/ul>\n",Coptotermes formosanus,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/4-wol.jpeg,","
The DNA PCR was not successful because the bands did not correlate to the Wolbachia control band.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/DSC10120-scaled.jpeg,",44.1564200000,-111.3927760000,09/29/2024,"
Very small approximately 1 mm in length, dark color. Found in bark of Douglas-fir tree with infestation markings, under bark above the phloem many same insects found.<\/p>\n",Leptothrips,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TBE,GelGreen,"","",Yes,CTGGAATTTGTGGATTATTTATAAGAATAATTATTCGTTTAGAATTAATACAACCAGGTAACGTTTTTTTATCTGGTCAAGCTTATAATGTAATTGTAACTAATCATGCTTTTATAATAATTTTTTTTACTATTATACCTATTATAATAGGAGGGTTTGGAAATTGATTAGTTCCTATTATACTAGGTAGACCAGATATATGTTACCCACGTATAAATAATATAAGATTTTGATTATTACCTCCTTCTCTATTTCTTCTCTTATTTAGAATAATTTTAGAAGAAGGTTCAGGAACTGGATGAACGGTTTATCCCCCTTTATCATCTTTAAGATCAGGAATTTCTGTTGATATAACTATTATTTCATTACATATTGCTGGTGTTTCTTCTTTATTAGGATCTATTAATTTTATTTCTACAGTATTTAACATATCTAATTTAAGAACTGAATATTATACTTTATTTGTTTGATCAATTGTAGTTACTTCTTTACTATTATTACTTTCTTTACCTGTATTAGCAGGTGCTTTAACAATATTATTGGTTGATCGAAATTTAAACACTAGTTATTTTGATCCTTCAGGTGGAGGAGATCCTACTTTATATCAACATTTATTTTGATT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-15-8.33.13-PM.png,",31.9708128,-81.15865149999999,08/29/2024,"
Time of day: 2:30pm – 3:10pm<\/p>\n
Weather conditions: 99 degrees – High 90 degrees Low 76 degrees – 68% Humidity\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a08 mph Wind – 20 Air Index<\/p>\n
Used a tray and jar to collect the insect. Found in a semi-dry environment; in the woods near a pond. The earwig was collected from a fallen tree branch.<\/p>\n",Dermaptera,,,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-15-8.16.37-PM-1.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/DSC10119-scaled.jpeg,",44.1564450000,-111.3929300000,09/29/2024,"
Very small approximately 1 mm in length, dark color. Found in bark of Douglas-fir tree with infestation markings, under bark above the phloem many same insects found.<\/p>\n
\n",Leptothrips,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TBE,GelGreen,"","",No,TGATCTGGAATTTGTGGATTATTTATAAGAATAATTATTCGTTTAGAATTAATACAACCAGGTAACGTTTTTTTATCTGGTCAAGCTTATAATGTAATTGTAACTAATCATGCTTTTATAATAATTTTTTTTACTATTATACCTATTATAATAGGAGGGTTTGGAAATTGATTAGTTCCTATTATACTAGGTAGACCAGATATATGTTACCCACGTATAAATAATATAAGATTTTGATTATTACCTCCTTCTCTATTTCTTCTCTTATTTAGAATAATTTTAGAGGAAGGTTCAGGAACTGGATGAACGGTTTATCCCCCTTTATCATCTTTAAGATCAGGAATTTCTGTTGATATAACTATTATTTCATTACATATTGCTGGTGTTTCTTCTTTATTAGGATCTATTAATTTTATTTCTACAGTATTTAACATATCTAATTTAAGAACTGAATATTATACTTTATTTGTTTGATCAATTGTAGTTACTTCTTTACTATTATTACTTTCTTTACCTGTATTAGCAGGTGCTTTAACAATATTATTGGTTGATCGAAATTTAAACACTAGTTATTTTGATCCTTCAGGTGGAGGAGATCCTACTTTATATCAACATTTATTTTGATTTTTTGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/DSC10119-scaled.jpeg,",44.1564450000,-111.3929300000,09/29/2024,"
Very small approximately 1 mm in length, dark color. Found in bark of Douglas-fir tree with infestation markings, under bark above the phloem many same insects found.<\/p>\n
\n",Leptothrips,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TBE,GelGreen,"","",Yes,GATCTGGAATTTGTGGATTATTTATAAGAATAATTATTCGTTTAGAATTAATACAACCAGGTAACGTTTTTTTATCTGGTCAAGCTTATAATGTAATTGTAACTAATCATGCTTTTATAATAATTTTTTTTACTATTATACCTATTATAATAGGAGGGTTTGGAAATTGATTAGTTCCTATTATACTAGGTAGACCAGATATATGTTACCCACGTATAAATAATATAAGATTTTGATTATTACCTCCTTCTCTATTTCTTCTCTTATTTAGAATAATTTTAGAGGAAGGTTCAGGAACTGGATGAACGGTTTATCCCCCTTTATCATCTTTAAGATCAGGAATTTCTGTTGATATAACTATTATTTCATTACATATTGCTGGTGTTTCTTCTTTATTAGGATCTATTAATTTTATTTCTACAGTATTTAACATATCTAATTTAAGAACTGAATATTATACTTTATTTGTTTGATCAATTGTAGTTACTTCTTTACTATTATTACTTTCTTTACCTGTATTAGCAGGTGCTTTAACAATATTATTGGTTGATCGAAATTTAAACACTAGTTATTTTGATCCTTCAGGTGGAGGAGATCCTACTTTATATCAACATTTATTTTGATTTTTTG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/DSC10120-scaled.jpeg,",44.1564200000,-111.3927760000,09/29/2024,"
Very small approximately 1 mm in length, dark color. Found in bark of Douglas-fir tree with infestation markings, under bark above the phloem many same insects found.<\/p>\n",Leptothrips,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TBE,GelGreen,"","",No,CTGGAATTTGTGGATTATTTATAAGAATAATTATTCGTTTAGAATTAATACAACCAGGTAACGTTTTTTTATCTGGTCAAGCTTATAATGTAATTGTAACTAATCATGCTTTTATAATAATTTTTTTTACTATTATACCTATTATAATAGGAGGGTTTGGAAATTGATTAGTTCCTATTATACTAGGTAGACCAGATATATGTTACCCACGTATAAATAATATAAGATTTTGATTATTACCTCCTTCTCTATTTCTTCTCTTATTTAGAATAATTTTAGAGGAAGGTTCAGGAACTGGATGAACGGTTTATCCTCCTTTATCATCTTTAAGATCAGGAATTTCTGTTGATATAACTATTATTTCATTACATATTGCTGGTGTTTCTTCTTTATTAGGATCTATTAATTTTATTTCTACAGTATTTAACATATCTAATTTAAGAACTGAATATTATACTTTATTTGTTTGATCAATTGTAGTTACTTCTTTACTATTATTACTTTCTTTACCTGTATTAGCAGGTGCTTTAACAATATTATTGGTTGATCGAAATTTAAACACTAGTTATTTTGATCCTTCAGGTGGAGGAGATCCTACTTTATATCAACATTTAT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/DSC10120-scaled.jpeg,",44.1564200000,-111.3927760000,09/29/2024,"
Very small approximately 1 mm in length, dark color. Found in bark of Douglas-fir tree with infestation markings, under bark above the phloem many same insects found.<\/p>\n",Leptothrips,Edwards Buffer,,Standard electrophoresis system,TBE,GelGreen,"","",Yes,TGATCTGGAATTTGTGGATTATTTATAAGAATAATTATTCGTTTAGAATTAATACAACCAGGTAACGTTTTTTTATCTGGTCAAGCTTATAATGTAATTGTAACTAATCATGCTTTTATAATAATTTTTTTTACTATTATACCTATTATAATAGGAGGGTTTGGAAATTGATTAGTTCCTATTATACTAGGTAGACCAGATATATGTTACCCACGTATAAATAATATAAGATTTTGATTATTACCTCCTTCTCTATTTCTTCTCTTATTTAGAATAATTTTAGAGGAAGGTTCAGGAACTGGATGAACGGTTTATCCCCCTTTATCATCTTTAAGATCAGGAATTTCTGTTGATATAACTATTATTTCATTACATATTGCTGGTGTTTCTTCTTTATTAGGATCTATTAATTTTATTTCTACAGTATTTAACATATCTAATTTAAGAACTGAATATTATACTTTATTTGTTTGATCAATTGTAGTTACTTCTTTACTATTATTACTTTCTTTACCTGTATTAGCAGGTGCTTTAACAATATTATTGGTTGATCGAAATTTAAACACTAGTTATTTTGATCCTTCAGGTGGAGGAGATCCTACTTTATATCAACATTTATTTTGATTTTTTGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/DSC10120-scaled.jpeg,",44.1564200000,-111.3927760000,09/29/2024,"
Very small approximately 1 mm in length, dark color. Found in bark of Douglas-fir tree with infestation markings, under bark above the phloem many same insects found.<\/p>\n
\n",Leptothrips,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TBE,GelGreen,"","",No,GTTTTTGATCTGGAATTTGTGGATTATTTATAAGAATAATTATTCGTTTAGAATTAATACAACCAGGTAACGTTTTTTTATCTGGTCAAGCTTATAATGTAATTGTAACTAATCATGCTTTTATAATAATTTTTTTTACTATTATACCTATTATAATAGGAGGGTTTGGAAATTGATTAGTTCCTATTATACTAGGTAGACCAGATATATGTTACCCACGTATAAATAATATAAGATTTTGATTATTACCTCCTTCTCTATTTCTTCTCTTATTTAGAATAATTTTAGAGGAAGGTTCAGGAACTGGATGAACGGTTTATCCTCCTTTATCATCTTTAAGATCAGGAATTTCTGTTGATATAACTATTATTTCATTACATATTGCTGGTGTTTCTTCTTTATTAGGATCTATTAATTTTATTTCTACAGTATTTAACATATCTAATTTAAGAACTGAATATTATACTTTATTTGTTTGATCAATTGTAGTTACTTCTTTACTATTATTACTTTCTTTACCTGTATTAGCAGGTGCTTTAACAATATTATTGGTTGATCGAAATTTAAACACTAGTTATTTTGATCCTTCAGGTGGAGGAGATCCTACTTTATATCAACATTTATTTTGATTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/DSC10120-scaled.jpeg,",44.1564060000,-111.3925370000,09/29/2024,"
Very small approximately 1 mm in length, dark color. Found in bark of Douglas-fir tree with infestation markings, under bark above the phloem many same insects found.<\/p>\n",Leptothrips,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TBE,GelGreen,"","",Yes,GTTTTTGATCTGGAATTTGTGGATTATTTATAAGAATAATTATTCGTTTAGAATTAATACAACCAGGTAACGTTTTTTTATCTGGTCAAGCTTATAATGTAATTGTAACTAATCATGCTTTTATAATAATTTTTTTTACTATTATACCTATTATAATAGGAGGGTTTGGAAATTGATTAGTTCCTATTATACTAGGTAGACCAGATATATGTTACCCACGTATAAATAATATAAGATTTTGATTATTACCTCCTTCTCTATTTCTTCTCTTATTTAGAATAATTTTAGAGGAAGGTTCAGGAACTGGATGAACGGTTTATCCTCCTTTATCATCTTTAAGATCAGGAATTTCTGTTGATATAACTATTATTTCATTACATATTGCTGGTGTTTCTTCTTTATTAGGATCTATTAATTTTATTTCTACAGTATTTAACATATCTAATTTAAGAACTGAATATTATACTTTATTTGTTTGATCAATTGTAGTTACTTCTTTACTATTATTACTTTCTTTACCTGTATTAGCAGGTGCTTTAACAATATTATTGGTTGATCGAAATTTAAACACTAGTTATTTTGATCCTTCAGGTGGAGGAGATCCTACTTTATATCAACATTTATTTTGATTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/DSC10120-scaled.jpeg,",44.1564060000,-111.3925370000,09/29/2024,"
Very small approximately 1 mm in length, dark color. Found in bark of Douglas-fir tree with infestation markings, under bark above the phloem many same insects found.<\/p>\n",Leptothrips,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TBE,GelGreen,"","",Yes,GTGGATTATTTATAAGAATAATTATTCGTTTAGAATTAATACAACCAGGTAACGTTTTTTTATCTGGTCAAGCTTATAATGTAATTGTAACTAATCATGCTTTTATAATAATTTTTTTTACTATTATACCTATTATAATAGGAGGGTTTGGAAATTGATTAGTTCCTATTATACTAGGTAGACCAGATATATGTTACCCACGTATAAATAATATAAGATTTTGATTATTACCTCCTTCTCTATTTCTTCTCTTATTTAGAATAATTTTAGAGGAAGGTTCAGGAACTGGATGAACGGTTTATCCTCCTTTATCATCTTTAAGATCAGGAATTTCTGTTGATATAACTATTATTTCATTACATATTGCTGGTGTTTCTTCTTTATTAGGATCTATTAATTTTATTTCTACAGTATTAAACATATCTAATTTAAGAACTGAATATTATACTTTATTTGTTTGATCAATTGTAGTTACTTCTTTACTATTATTACTTTCTTTACCTGTATTAGCAGGTGCTTTAACAATATTATTGGTTGATCGAAATTTAAACACTAGTTATTTTGACCCTTCAGGTGGAGGAGATCCTACTTTATATCAACATTTATTTTGATTTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/DSC10116-scaled.jpeg,",44.1564060000,-111.3925370000,09/29/2024,"
Very small approximately 1 mm in length, dark color. Found in bark of Douglas-fir tree with infestation markings, under bark above the phloem many same insects found.<\/p>\n",Leptothrips,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TBE,GelGreen,"","",Yes,TGTGGATTATTTATAAGAATAATTATTCGTTTAGAATTAATACAACCAGGTAACGTTTTTTTATCTGGTCAAGCTTATAATGTAATTGTAACTAATCATGCTTTTATAATAATTTTTTTTACTATTATACCTATTATAATAGGAGGGTTTGGAAATTGATTAGTTCCTATTATACTAGGTAGACCAGATATATGTTACCCACGTATAAATAATATAAGATTTTGATTATTACCTCCTTCTCTATTTCTTCTCTTATTTAGAATAATTTTAGAGGAAGGTTCAGGAACTGGATGAACGGTTTATCCCCCTTTATCATCTTTAAGATCAGGAATTTCTGTTGATATAACTATTATTTCATTACATATTGCTGGTGTTTCTTCTTTATTAGGATCTATTAATTTTATTTCTACAGTATTTAACATATCTAATTTAAGAACTGAATATTATACTTTATTTGTTTGATCAATTGTAGTTACTTCTTTACTATTATTACTTTCTTTACCTGTATTAGCAGGTGCTTTAACAATATTATTGGTTGATCGAAATTTAAACACTAGTTATTTTGATCCTTCAGGTGGAGGAGATCCTACTTTATATCAACATTTATTTTGATTTTTTG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/DSC10116-scaled.jpeg,",44.1564060000,-111.3925370000,09/29/2024,"
Very small approximately 1 mm in length, dark color. Found in bark of Douglas-fir tree with infestation markings, under bark above the phloem many same insects found.<\/p>\n
\n",Leptothrips,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TBE,GelGreen,"","",No,AATTTGTGGATTATTTATAAGAATAATTATTCGTTTAGAATTAATACAACCAGGTAACGTTTTTTTATCTGGTCAAGCTTATAATGTAATTGTAACTAATCATGCTTTTATAATAATTTTTTTTACTATTATACCTATTATAATAGGAGGGTTTGGAAATTGATTAGTTCCTATTATACTAGGTAGACCAGATATATGTTACCCACGTATAAATAATATAAGATTTTGATTATTACCTCCTTCTCTATTTCTTCTCTTATTTAGAATAATTTTAGAGGAAGGTTCAGGAACTGGATGAACGGTTTATCCTCCTTTATCATCTTTAAGATCAGGAATTTCTGTTGATATAACTATTATTTCATTACATATTGCTGGTGTTTCTTCTTTATTAGGATCTATTAATTTTATTTCTACAGTATTTAACATATCTAATTTAAGAACTGAATATTATACTTTATTTGTTTGATCAATTGTAGTTACTTCTTTACTATTATTACTTTCTTTACCTGTATTAGCAGGTGCTTTAACAATATTATTGGTTGATCGAAATTTAAACACTAGTTATTTTGATCCTTCAGGTGGAGGAGATCCTACTTTATATCAACATTTATTTTGAT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/DSC10116-scaled.jpeg,",44.1564060000,-111.3925370000,09/29/2024,"
Very small approximately 1 mm in length, dark color. Found in bark of Douglas-fir tree with infestation markings, under bark above the phloem many same insects found.<\/p>\n
\n",Leptothrips,Edwards Buffer,Whole arthropod,Standard electrophoresis system,TBE,GelGreen,"","",No,TTGATCTGGAATTTGTGGATTATTTATAAGAATAATTATTCGTTTAGAATTAATACAACCAGGTAACGTTTTTTTATCTGGTCAAGCTTATAATGTAATTGTAACTAATCATGCTTTTATAATAATTTTTTTTACTATTATACCTATTATAATAGGAGGGTTTGGAAATTGATTAGTTCCTATTATACTAGGTAGACCAGATATATGTTACCCACGTATAAATAATATAAGATTTTGATTATTACCTCCTTCTCTATTTCTTCTCTTATTTAGAATAATTTTAGAAGAAGGTTCAGGAACTGGATGAACGGTTTATCCCCCTTTATCATCTTTAAGATCAGGAATTTCTGTTGATATAACTATTATTTCATTACATATTGCTGGTGTTTCTTCTTTATTAGGATCTATTAATTTTATTTCTACAGTATTTAACATATCTAATTTAAGAACTGAATATTATACTTTATTTGTTTGATCAATTGTAGTTACTTCTTTACTATTATTACTTTCTTTACCTGTATTAGCAGGTGCTTTAACAATATTATTGGTTGATCGAAATTTAAACACTAGTTATTTTGATCCTTCAGGTGGAGGAGATCCTACTTTATATCAACATTTATTTTGATTTTTTGGTC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/DSC10116-scaled.jpeg,",44.1564060000,-111.3925370000,09/29/2024,"
Very small approximately 1 mm in length, dark color. Found in bark of Douglas-fir tree with infestation markings, under bark above the phloem many same insects found.<\/p>\n",Leptothrips,Edwards Buffer,,Standard electrophoresis system,TBE,,"","",No,GAATCTGTGGATTATTTATAAGAATAATTATTCGTTTAGAATTAATACAACCAGGTAACGTTTTTTTATCTGGTCAAGCTTATAATGTAATTGTAACTAATCATGCTTTTATAATAATTTTTTTTACTATTATACCTATTATAATAGGAGGGTTTGGAAATTGATTAGTTCCTATTATACTAGGTAGACCAGATATATGTTACCCACGTATAAATAATATAAGATTTTGATTATTACCTCCTTCTCTATTTCTTCTCTTATTTAGAATAATTTTAGAGGAAGGTTCAGGAACTGGATGAACGGTTTATCCTCCTTTATCATCTTTAAGATCAGGAATTTCTGTTGATATAACTATTATTTCATTACATATTGCTGGTGTTTCTTCTTTATTAGGATCTATTAATTTTATTTCTACAGTATTTAACATATCTAATTTAAGAACTGAATATTATACTTTATTTGTTTGATCAATTGTAGTTACTTCTTTACTATTATTACTTTCTTTACCTGTATTAGCAGGTGCTTTAACAATATTATTGGTTGATCGAAATTTAAACACTAGTTATTTTGATCCTTCAGGTGGAGGAGATCCTACTTTATATCAACATTTAT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_8352-scaled.jpeg,",33.9895091670,-84.4403878535,11/12/2024,"
Moved around very fast when tried to catch the ant; orangish color; has dark spots on back of body; black eyes.<\/p>\n",Pogonomyrmex occidentalis,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/6-arth-1.jpeg,","
We pipetted the samples into the gel wells and added buffer. We performed the procedure correctly; no bands were present in the gel. The sample was not sent for sequencing.<\/p>\n",Unknown,n/a,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_8338-1-scaled.jpeg,",33.9895219684,-84.4404155928,11/12/2024,"
Rolled into a ball when found: it was walking on a leaf in the ground; scaly; has many legs and 2 antennas.<\/p>\n",Armadillidium vulgare,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/04/6-arth.jpeg,","
We pipetted the samples into the gel wells and added buffer. We performed the procedure correctly, but the bands were not visible in the gel. The sample was not sent for sequencing.<\/p>\n",Unknown,n/a,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_5923-scaled.jpeg,",33.9887861444,-84.4397402744,11/12/2024,"",Nylanderia fulva,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_9002.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_9003.jpeg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3802-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3798-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3716-scaled.jpeg,",33.9888793036,-84.4398006259,11/12/2024,"
The specimen was found covered under a pile of leaves on wet soil. The area was open and not significantly crowded by rocks or trees.<\/p>\n",Arthropoda,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"","
Gel results for both Wolbachia\u00a0<\/em>and CO1 were unclear. Missing steps during the centrifuging process may have been a main contributor to inconclusive results.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3805-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3721-scaled.jpeg,",33.9888793036,-84.4398006259,11/12/2024,"
Environment was a forested area. Found near medium-sized rocks and on moist area under leaves and branches. No other ants were apparent or seen near the ant.<\/p>\n",Arthropoda,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/01/IMG_5507-scaled.jpeg,","
Gel results for determining presence of Wolbachia<\/em> in this specimen were not conclusive. Unclear bands may have been due to error during PCR, specifically with the centrifuging timeline during the process. Clear bands were present for the CO1 gel, and results were sent for specimen identification.<\/p>\n",Unknown,TTGTTTTTTCGTTGCATTTGATCANGAATAATTGGTTCTTCAATAAGAATAATTATTCGATTAGAATTAGGATCCCCTGATTCACTAATTCTTAATGATCAAACCTTTAATACTATTGTTACAAGCCATGCTTTTATTATAATCTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAACTTCTTAGTACCCTTAATACTTGGATCTCCCGATATAGCCTACCCACGCATAAATAACATAAGATTTTGATTGCTACCCCCTTCAATCTCTCTATTAATCTTAAGAAACTTTATTAATGAAGGATCAGGTACAGGTTGAACTATTTACCCCCCTCTATCGTCTAATACCTTTCATAGTGGTCCCTCTATTGATTTAACTATTTTTTCCCTTCATATTGCCGGAATATCATCAATTCTAGGAGCAATCAATTTTATTTCAACAATTTTAAATATACATAATTCTAATATTTCTTTAGATAAAATTCCTCTATTAGTATGATCTATTCTTATCACTGCTATTCTCCTTCTCCTCTCCCTTCCTGTTCTTGCAGGAGCTATTACAATACTACTTACAGACCGAAATCTCAATACTTCATTCTTTGATCCCTCGGGAGGAGGAGACCCTATTCTATACCAACACTTATTTTGATTTTTTGGTCACCTTGAAAGTTAAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/7-1-scaled.jpg,",38.9339378000,-77.1773240000,09/04/2024,"",Eunemobius carolinus,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-14-at-12.26.19-AM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-14-at-12.39.35-AM.png,","",Yes,CAAATTTATAATGTAATTGTAACATCTCATGCTTTTGTAATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAACTGATTAGTTCCTTTAATATTAGGCGCACCAGACATAGCATTTCCCCCAATAAATAATATAAGATTCTGATTACTACCCCCTTCATTAACTCTCCTCTTATCTAGAAAAATATTAGAAAATGGTGCAGGAACAGGATGAACAGTGTACCC,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/7-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/lovebug-1-scaled.jpg,",,,,"",Diptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screen-Shot-2024-11-14-at-3.52.25-PM.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_0671.jpeg,",38.9674531463,-77.0678376409,09/06/2024,"
I found the arthropod in a small park under a rock during the fall. it was about 75 degrees outside. The arthropod was in a terrestrial habitat. It is gray, and has many legs.<\/span><\/p>\n
<\/p>\n",Nomophila nearctica,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0684.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0685.jpeg,","
DNA Extraction: I made sure to crush the arthropod really well, the arthropod was small, so no dissection was necessary<\/p>\n
Gel Electrophoresis, arthropod CO1:<\/p>\n
1 – DNA ladder<\/p>\n
2 – A4 DNA<\/p>\n
3 – A5 DNA<\/p>\n
4- A6 DNA<\/p>\n
5 – Arthropod positive control<\/p>\n
6 – Arthropod negative control<\/p>\n
7 –\u00a0 water<\/p>\n
Analysis: all controls showed expected bands, and the sample showed a arthropod CO1 band.<\/p>\n
Gel electrophoresis, Wolbachia:<\/p>\n
1 – DNA ladder<\/p>\n
2 – A4 DNA<\/p>\n
3 – A5 DNA<\/p>\n
4- A6 DNA<\/p>\n
5 – Wolbachia positive control<\/p>\n
6\u00a0– wolbachia negative control<\/p>\n
7 –\u00a0 water<\/p>\n
Analysis: all controls showed expected bands, and the sample did not show a wolbachia band.<\/p>\n
<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_8519-scaled.jpeg,",41.9021492000,-71.4569778000,11/01/2024,"
Found underneath the bark of a dead log<\/p>\n",Diplopoda,Blood and Tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3282-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3275-scaled.jpeg,","",Yes,,,, "",41.9021486,-71.4569787,11/14/2024,"",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_8266-scaled.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_8620-scaled.jpeg,",41.90138814815067,-71.45624919714729,11/01/2024,"
It was hopping in a feild near a different building. It was caught while resting<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_8636-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_6118-scaled.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Wolf-Spider-scaled.jpg,",41.9021416000,-71.4569759000,11/01/2024,"
Running fast trying to hide under leaves, near a fallen tree.<\/p>\n",Arachnida,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Gel-1-Arthropod-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Gel-2-Wolbachia-scaled.jpg,","",No,,,, "",41.9018514626,-71.4566174469,11/01/2024,"
The ant I collected was found in the woods in a decaying tree. It was similar to the Australian meat ant. I was unable to get a pictture of it.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_8633-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_6117-scaled.jpeg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/pill-bug-scaled.jpg,",41.9021429000,-71.4569761000,09/18/2024,"
Found under a rock, oval-shaped, trying to get away and hide under leaves.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Rear half,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/unnamed-8-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/unnamed-7.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG_8125-scaled.jpeg,",41.9005527584,-71.4591820711,09/19/2024,"
The ant was found in a decaying log in the forest behind the school. It looks similar to a me at any from Australia. It has quiet a hard shell and moved too fast to take a picture of it in the log.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3612-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3613-scaled.jpeg,",47.4436639727,8.5292495239,08/18/2024,"
I found it on a grass field next to my house.<\/p>\n",Suillia affinis,Instagene Matrix,Whole arthropod,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_2443-1.jpeg,","
Reasons why it did not work the first time(Picture 1):<\/p>\n
– Foam reaction<\/p>\n
– to long in centrifugator<\/p>\n
– to much of the sample<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/27_Arthropod-CO1F-1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_7539.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_7540.jpg,",433528.9000000000,702011.0000000000,10/10/2024,"
brown in color with different shades, missing a leg<\/p>\n",Lepidoptera,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/download.jpeg,",,,08/28/2024,"
\n
Found at the base of a tree<\/li>\n
Collected with forceps, and placed into a glass vile<\/li>\n
<\/li>\n<\/ul>\n",Dermaptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Jayden-Singletary-Jaida-Bedgood-1-scaled.jpg,","
\n
DNA extraction kit of in-house reagents was used.<\/li>\n<\/ul>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_7760-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_7761-1-scaled.jpg,",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-12-111823.png,",32.0808989,-81.091203,08/27/2024,"
Insect was found in a hot sunny area near flowers.<\/p>\n",Lepidoptera,,Partial abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-10-29-154133.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Unknown,,,, "",47.540831,8.473612,11/20/2024,"
It was caught in the pool before it drowned.<\/p>\n",Sciaridae,pcr,Abdomen,MiniPCR,Other,,"","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/41_Arthropod-CO1F_finalversion.N.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/insect-drawing.png,",31.97897254499886,-81.16338849640023,08/27/2024,"
\n
Environment:
\n<\/b>– Grassy, open area.
\n– Shrubs and trees.
\n– Mildly hot weather.
\nCollection Method:
\n<\/b>– <\/b>Use a Sweep net .
\n– Put them in a jar.
\n– Ethanol Preservation<\/li>\n<\/ul>\n",Hemiptera,,Partial abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Labeled-gel.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,AGCAGGTATAGTAGGATCCGCTATAAGACTAATTATCCGAATCGAACTAG GACAACCAGGAAGATTCATTGGAGATGATCAAATTTATAACGTAATCGTA ACAGCTCACGCCTTCGTAATAATTTTCTTCATAGTAATACCTATTATAAT TGGAGGATTTGGAAACTGACTAGTTCCCCTAATAATCGGAGCTCCCGACA TAGCATTCCCTCGTATAAATAACATAAGATTTTGATTATTACCTCCTTCA TTAACATTATTAATAATAAGTAGACTAGCAGAATCAGGAGCTGGAACCGG ATGAACAGTTTACCCTCCTTTATCAAGTAATTTATCCCACAGAGGAGCAT CCGTAGACTTAGCAATTTTTTCACTCCACTTAGCCGGAGTATCTTCAATC TTGGGAGCAGTAAATTTTATTTCCACAATCATTAATATACGACCCGCAGG AATAATCCCTGAACGAATTCCTTTATTTGTATGATCAGTAGGGATTACTG CCTTACTTCTACTACTATCATTACCTGTGCTAGCAGGTGCTATTACAATA TTATTAACTGATCGAAATTTTAACACATCATTTTTCGATCCCTCAGGTGG AGGAGACCCTATTTTATACCAACATTTATTTTGATTTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Alaiyah-Mcphail-Presentation-Draft-.png,",31.9708128,-81.15865149999999,01/08/2025,"
\n
Climate: Hot summer day\u00a0<\/b><\/li>\n<\/ul>\n
\n
Time: 1:49 P.M\u00a0<\/b><\/li>\n<\/ul>\n
\n
Location: Grass (Outside of library), Tree near Learning Commons, sidewalk by fountain, and International gardens.\u00a0<\/b><\/li>\n<\/ul>\n
\n
Environment (Habitat): Natural Environment\u00a0<\/b><\/li>\n<\/ul>\n
\n
Method: Sweep net\u00a0<\/b><\/li>\n<\/ul>\n
<\/p>\n",Odonata,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_4657-scaled.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/2-scaled-e1730990498876.jpg,",38.8818096136,-77.1395234971,09/03/2024,"
This arthropod was found outside near my front porch on a cool night.<\/p>\n",Dichaetophora,DNeasy (Qiagen),Whole arthropod,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screen-Shot-2024-11-08-at-23.02.07.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screen-Shot-2024-11-08-at-23.02.02-1.png,","",No,CNTTTTGGGGCNTGAGCCGGAATGGTTGGAACTTCTTTAAGAATTTTAATCCGAGCAGAATTNGGGNN NCCTGGGGCTTTAATTGGAGATGATCAAATTTATAATGTAATTGTAACTGCTCACGCTTTTGTAATAATTTTTTTTATAG TTATACCAATTATAATTGGAGGATTTGGAAATTGACTACTTCCTTTAATATTAGGAGCTCCCGATATCGCTNTTCCNCGA ATAAATAATATAAGTTTCTGACCGCTTCCCCCTTCTNTTACTTTACTTTTAGTANGTNNAATANTTGAAAACGGAGCGGG GACACGGTGAACTGCCTATCCCCCCCCCTCNCCTCCCCTNGCNCATGGAGGGCCTTCCATAAATTTAGCTCTTTTTANAC TTCNTTTNNCNCGANNCTATTCCGNTTNGGAGCTGAAAATNNCCCTACTCCACTTACTAATAGACGATCAACNGGAATCA CNTTTGATCTGATACCNTTATTTGTATGATCNCTTGCTATTACTGCTTTATTACTCCTTCCCTCTNNACCTGATCTANAN GGANCTATTACCATGTTATTAACAGANANNAATTTANTTACTTCATTTTTTGANCCTGCNNGAGGANGACATCCTTNTTT TTATTNANNTNNNTTTNGACTNTTTTGGTCACCCNNAAANTTTAAN,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/2-CO1F-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Insect-Image-Enhanced.jpg,",31.9678867000,-81.1533760000,08/27/2024,"
\n
Kinsey (past lab partner) and I collected insects on August 27, 2024\u00a0<\/span><\/li>\n
Insects were collected from 1-1:30pm, my specific insect sample DERMAHF24-AN, was collected around 1:23<\/span><\/li>\n
Most insects were collected from the \u2018International Gardens\u2019 across from The Learning Commons on campus\u00a0<\/span><\/li>\n
Most insects collected were inhabiting shrubs or grass, however some insects were collected airborne\u00a0<\/span><\/li>\n
Insects were collected using a net and jar. Kinsey operated the net and forceps, while I enclosed the insects in the jar when captured\u00a0<\/span><\/li>\n<\/ul>\n",Dermaptera,,Rear half,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/GEL-IMAGE-scaled.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,TGGATACTAGGATCATCAATGAGACTTATTATTCGAATAGAATTAAGATC CCCAGGATCATGAATTAGCAATGATCAAATTTATAATACAATTGTTACTA GTCATGCATTCCTAATAATTTTTTTTATAGTTATACCATTTTTAATTGGA GGATTTGGAAATTGGCTTATTCCTTTAATACTAGGATCACCTGATATAGC ATTCCCCCGAATAAATAATATTAGATTTTGATTACTTCCTCCCTCATTAT TTATACTTTTATTAAGAAATTTATTTTATCCAAGACCAGGAACTGGATGA ACAGTATATCCACCATTATCAGCATATTTATATCATTCTTCACCTTCAGT AGATTTTGCAATTTTTTCTCTTCATATATCAGGAATTTCCTCAATTATAG GATCATTAAACTTAATAGTTACAATTATAATAATAAAAAATTTTTCTATA AATTATGACCAAATTTCATTATTTCCATGATCAGTTTTTATTACAGCAAT TTTATTAATTATATCATTACCTGTATTAGCTGGAGCAATTACTATACTAT TATTTGATCGAAATTTTAATACATCATTTTTCGATCCTATAGGAGGTGGA GATCCAATTCTTTATCAACATTTATTTTGATTTTTTGG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3258.jpg,",,,08/28/2024,"
\n
Time<\/strong>: 2:31 PM<\/li>\n
Habitat<\/strong>: Inside a tea olive bush on the side of the Science center<\/li>\n
Method<\/strong>: Beating stick and net<\/li>\n<\/ul>\n",Hymenoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3211.jpg,","
A DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_7492-scaled.jpg,",,,08/27/2024,"
Insect was in the grass <\/span>along with other fallen <\/span>leaves.<\/span>\u200b The sweep net method was used to capture insect.<\/span><\/p>\n",Hemiptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_8330-1.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Diptera-Drawing-scaled.jpg,",31.9812538452,-81.1574111796,08/27/2024,"
Caught Aug 27, 2024 from 1:00 to 1:20 PM. Collected in tall grass near a creek.<\/p>\n",Plecia nearctica,,Partial abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-10-31-125420.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,AACTTCACTAAGTATATTAATTCGAACAGAATTAAGCCATCCAGGATTTT TTATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCCCATGCTTTT ATTATAATTTTTTTTATAGTTATACCAATTATAATTGGTGGATTTGGAAA CTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTTCCTCGAA TAAATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTCTTCTA ATAAGAAGCATAGTTGAAAACGGGGCTGGTACTGGATGAACAGTATATCC CCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTTAGCAA TTTTTTCTCTTCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTGTTAAT TTTATTACTACAGTTATTAATATGCGATCTACTGGAATTACATTTGATCG TATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACTTCTCC TATCCCTCCCAGTTTTAGCCGGGGCTATTACTATACTATTAACAGATCGA AATTTAAATACAAGTTTCTTTGACCCAGCAGGAGGGGGAGACCCTATTTT ATACCAACATTTATTTTGATTTTTTGGTC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/NIU-21.jpg,",41.9408626,-88.7686288,08/14/2024,"
small beetle<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/NIU-20--scaled.jpg,",41.9408626,-88.7686288,08/08/2024,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/NIU-19-scaled.jpg,",41.9408626000,-88.7686288000,07/30/2024,"
bee<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/NIU-18-scaled.jpg,",41.9408626,-88.7686288,07/30/2024,"
bee<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/NIU-17.jpg,",41.9408626,-88.7686288,08/17/2024,"
fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/NIU-16.jpg,",41.9315691,-88.7636562,10/06/2024,"
ant<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/NIU-15-scaled.jpg,",41.9315698,-88.763647,,"
earwig<\/p>\n",Dermaptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/NIU-14.jpg,",41.9408626,-88.7686288,09/12/2024,"
fruit fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/NIU-13.jpg,",41.9408626,-88.7686288,09/12/2024,"
rove beetle<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/NIU-12--scaled.jpg,",41.9408626,-88.7686288,08/08/2024,"
yellow longhorned beetle<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/NIU-11-scaled.jpg,",41.9408626,-88.7686288,08/08/2024,"
Compost fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/NIU-10.jpg,",41.9408626,-88.7686288,09/12/2024,"
picnic bugs<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/3-scaled.jpg,",38.9290563467,-77.0708196048,09/04/2024,"
The arthropod was feeding on a fellow student. It was late summer about 75 degrees Fahrenheit and 3:30 pm.<\/p>\n",Aedes albopictus,DNeasy (Qiagen),body without wings,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-07-at-10.00.21 AM.png,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/shared-image-3.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/shared-image-4.jpg,",47.2829000000,8.3048000000,08/25/2024,"
Behind the house, lying on the gras without the ability to fly. I caught it with a glas.<\/p>\n",Polistes,Instagene Matrix,Partial abdomen,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Wolb-2024-saz19-Davidstern02-150x150-1.png,","
strong faint, in the picture its the 4th lane<\/p>\n",No,TAAGANTAATAATTCGATTAGAATTAGGAACACCATCTTCAATTATTAATAATGATCAATTTTATAATTCAATTATTACA GCCCATGCTTTAGTAATAATTTTTTTTATAGTTATACCATTTATAATTGGAGGTTTTGGAAATTGATTAATTCCTATAAT ACTTGGAGCCCCTGATATAGCTTTCCCACGAATAAATAATATAAGATTTTGACTTTTACCACCCTCATTATTTTTGCTTA TTATAAGAAACATAATAAAAATAGGAGTAGGAACAGGATGAACTTTATACCCACCTTTATCTTCAAATATTGGACATAAT TCACCTTCTGTTGATTTATCAATTTTTTCTCTTCATATTGCTGGAATTTCATCCATTATAGGAGCTATTAATTTTATTGT AACAATTTTAAATATACATATTAAAACCCATTCATTAAATTTTTTACCTTTATTTACATGATCTGTTTTAATTACAGCAA TTTTACTTTTATTATCTTTACCTGTCTTAGCAGGAGCAATCACTATACTATTAACTGATCGAAATTTAAATACATCTTTT TTTGATCCTACAGGAGGAGGTGANCCAATTTTATTTCAACATCTATTTTGANTTTTTG,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/20_Arthropod-CO1F-Davidstern.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Image-4-2-scaled.jpeg,",47.4870258087,8.5021043591,08/24/2024,"
caught on dining table<\/p>\n",Diptera,Instagene Matrix,Rear half,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Image-2.png,","
The slots were placed at the wrong place, and there wasn’t enough blue tracing dye in the samples.<\/p>\n",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/75_Wolbachia-WSpecF-1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_4164-scaled.jpeg,",31.9708128,-81.15865149999999,08/28/2024,"
Time of Collection: 2:54<\/p>\n
Method of Collection: Sweeping Net – Waving a net back and forth around tall patches of grass<\/span><\/p>\n",Diptera,,In front of University Building,Standard electrophoresis system,TAE,Other,"","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,CO1 gene,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/tempImagehDHYIt.jpg,",31.9794357068,-81.1609938539,08/28/2024,"
In temperate and tropical climates, cicadas reside in trees, shrubs, and subterranean tunnels while they are nymphs. Adults are frequently spotted in the canopy. Wolbachia, a bacterium that controls reproduction through mechanisms like cytoplasmic incompatibility, affects mating patterns and the spread of infected individuals, and is found in many cicada species. Cicadas are well-known for their recurring appearance in enormous “broods,” which frequently occur in cycles of 13 or 17 years and involve billions of individuals emerging at once. Males use a specialized membrane called a tymbal to create loud buzzing calls that can reach 120 dB in order to attract females. Although they only live a brief time as adults, cicadas spend years of their lives underground as nymphs, feasting on tree roots.<\/p>\n",Hemiptera,,,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_7487-scaled.jpeg,","
“DNA extraction kit of in-house reagents was used.”<\/p>\n",No,GTAGGCACATCATTAAGTATTTTAATTCGAACAGAACTAGGACAACCAGG CTATTTAATTGGAGATGATCAAACTTATAATGTTATCGTAACCGCACATG CATTTATCATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTT GGAAATTGATTAGTACCATTAATATTGGGGGCTCCAGATATAGCCTTCCC ACGAATAAACAATATAAGATTTTGACTTCTACCCCCATCATTAACCCTTT TATTAACCAGAAGAATAGTTGAAAATGGTGCAGGAACAGGATGAACAGTA TACCCACCTCTATCAACAGGAATCGCTCACGCGGGAGCATCCGTCGATTT AGCTATCTTCTCACTACATTTAGCAGGAATTTCCTCAATTCTAGGGGCTG TAAATTTTATCACTACCATAATTAATATACGAGCTCCAGGAATATCACTA GACCAAACACCATTATTTGTTTGAGCAGTCGGAATTACAGCTCTTCTATT ATTATTATCCTTACCAGTTTTAGCTGGTGCTATTACAATATTGCTTACAG ATCGAAATTTAAATACATCATTTTTTGATCCAGCGGGAGGAGGGGATCCA ATCTTATATCAACATCTATTTTGATTTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_2193-scaled.jpeg,",36.1444896,-86.8011462,08/31/2024,"",,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_5328.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_8080.jpeg,",31.9815680000,-81.1499520000,08/28/2024,"
Caught outside school on a warm day, caught by hand after finding on the concrete.<\/p>\n",Hymenoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_7988.jpeg,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/waspish.jpg,",,,08/28/2024,"
Found in bushes outside Lane Library in GSU. Used “catch and flip” method with the sweep net.<\/p>\n",Hymenoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/gelimage.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,TGCAGCTATAAGAATAATTATTCGATTAGAACTAGGAACACCTGGAAAAT TCATTGGTAATGATCAAATTTATAATACTTTAGTTACTGCTCACGCATTT ATCATAATTTTTTTCATAGTTATACCATTTATAATTGGAGGATTTGGAAA TTGATTAATTCCATTAATATTAACAGCTCCTGATATAGCCTTTCCCCGTA TAAATAATATAAGATTTTGATTACTACCTCCTTCATTAATTTTTTTATTA TCAAGTAGTATTATTGGTTCAGGTGTAGGAACAGGTTGAACCCTTTACCC TCCTTTATCATCTATTATTGGACATAATTCTCCATCAGTAGATTTAAGAA TTTTTGCTTTACATATTGCTGGAGTATCATCTATTATAGGAGCTATTAAT TTTATTGTAACAATTTTAAATATACATACTAAAAATTCTTCCTTAAATTC AATCCCACTTTTTGCTTGATCTGTTCTTATTACTGCAATTCTATTACTTC TATCTTTACCAGTATTAGCTGGAGCCATCACCATATTATTAACTGATCGA AATTTTAATACATCATTTTTTGACCCTGCTGGAGGAGGTGATCCAATCCT TTATCAACATTTATTTTGATTTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_5890-scaled.jpeg,",,,08/28/2024,"
Found on sunny day by a tree at Georgia Southern Armstrong Campus, Science Center.<\/p>\n
It was 2pm and 89 degrees outside.<\/p>\n",Coleoptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-06-143511.png,","
A DNA extraction kit of in-house reagents was used.<\/p>\n",No,N/A,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_9113-scaled.jpeg,",31.9708128,-81.15865149999999,11/06/2024,"
Used a sweeping net to collect the insec and collected around 2:00pm in a grassy area.<\/p>\n",Diptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_2538.jpeg,","
DNA Extraction kit of in-house reagents was used.<\/p>\n",No,,,, "",31.9708128,-81.15865149999999,08/28/2024,"",Coleoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_6830.jpg,","",No,,,, "",31.9708128,-81.15865149999999,08/28/2024,"",Hymenoptera,none,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3943.jpg,","",No,ATTAGGTTCATCTATAAGATTAATTATTCGTATAGAACTAGGTATAGCAG GTTCATTACTTAAAAATGAACAATTATTTAATTCAATCGTTACTTCACAT GCATTCGTAATAATTTTCTTTTTTATTATGCCTCTAATAATTGGAGGTTT TGGCAATTGATTAATTCCTCTAATATTGGGAGTACCTGATATAGCTTTCC CTCGATTAAATAATTTAAGATTTTGACTATTACCTCCCTCTCTTATTTTA TTAATTTTTTCTAACTTTGTAGGAAAAGGAATTGGGACAGGATGAACTAT TTATCCCCCACTATCTAATTCTATTTACCATACTGGTATAGCAGTAGATT TATTAATTTATTCCCTACATTTAAGAGGAATTTCTTCAATTCTAGGCTCA ATTAATTTTATTGTAACAATTTTTAAAATACATAATTCTAATATTAAAAT ACATTTAGTAATATTATTTCCTTGATCTATTTATATTACTACAATTTTAT TATTACTTTCATTACCAGTTTTAGCTGGGGCAATTACTATACTTTTATTT GATCGAAACTTTAATACTTCATTTTTTGATCCCTCAGGAGGAGGAGATCC AGTTTTATATCAACATTTATTTTGATTTTTTGGTC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_6121.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_6120.jpeg,",42.1163703059,-76.2760169516,09/09/2024,"
Formicidae colony found near the Owego creek under nearby rocks. Specimens were alive at the time of collection.<\/p>\n",Formicidae,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_6139.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_6140.jpeg,","\n\n\n
One of our protocol issues may have been cross-contamination that occurred while handling our micro centrifuge and polymerase chain reaction tubes. Additionally, our Arthropod versus Wolbachia samples were labeled indistinctly. Our gel electrophoresis yielded an abundance of noise, leading us to repeat the experiment. We attempted a second trial but still, our results were rather inconclusive due to our errors with labeling our tubes, and a lack of remaining solution within the tubes.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n",No,None,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Make-a-drawing-of-any-anatomical-feature-that-you-used-to-identify-your-insect-to-2-scaled-e1730865657273.jpg,",31.9762050990,-81.1601320794,08/27/2024,"
\n
collected by skimming a pond with a D-net<\/li>\n
pond surrounded by trees and grass; full of other organisms (bugs, snails, etc)<\/li>\n
collected around 1:15pm<\/li>\n<\/ul>\n",Nepidae,,Partial abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/EvaKatie_EdenJamiyah-1-scaled-e1730866044718.jpg,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,AGGAACTTCCTTAAGATGACTTATCCGAATCGAACtacgcctgcCAGGAT CATTCATTGGTGATGATCAAATTTATAATGTAGTAGTAACTGCCCATGCA TTCATTATAATCTTTTTTATAGTGATGCCAATTATGATCGGAGGATTTGG TAACTGACTAGTTCCTTTAATACTGGGGGCCCCAGATATAGCATTCCCAC GAATAAATAACATAAGATTCTGATTACTGCCCCCCTCACTAACTCTTCTT TTATCAAGTAGAATCGCCGAAAGAGGGGCCGGAACTGGATGAACAGTATA TCCTCCCCTATCTACTAATATTTCACACAGTGGAGCATCCGTAGATTTAG CTATTTTTTCATTACATTTAGCTGGTGTTTCCTCAATTTTAGGTGCTGTT AATTTTATTTCAACAATTATTAACATGCGAGCCACAGGAATAACCTCCGA CCGAATACCTTTATTTGTATGATCCGTAGGTATTACCGCCCTTCTTTTAC TTTTATCACTACCTGTACTTGCCGGAGCAATTACTATACTATTAACAGAT CGAAATTTAAATACCTCATTTTTCGATCCAGCAGGAGGAGGGGACCCAAT TCTTTATCAACACTTGTTTTGATTTTTTG,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_7726-scaled.jpeg,",31.9771910443,-81.1622397808,08/27/2024,"",Lepidoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/EvaKatie_EdenJamiyah-scaled.jpg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_1880.jpg,",31.9794787215,-81.1620197567,08/27/2024,"
Environment: Dry grass in a very green area with lots of fields and bushes<\/p>\n
Temperature: Hot and humid<\/p>\n
Time: 1:05 in the afternoon<\/p>\n",Diptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Gel-electrophoreis-.png,","
DNA extraction kit of in house reagents was used.<\/p>\n",No,AGTATATTAATTCGAACAGAATTAAgccaTCCAGGATTTTTTATTGGTGA TGATCAAATTTATAATGTAATTGTTACAGCCCATGCTTTTATTATAATTT TTTTTATAGTTATACCAATTATAATTGGTGGATTTGGAAACTGATTAGTT CCATTAATATTAGGAGCCCCTGATATAGCTTTTCCTCGAATAAATAATAT AAGATTTTGACTTTTACCACCTTCTTTATCCCTTCTTCTAATAAGAAGCA TAGTTGAAAACGGGGCTGGTACTGGATGAACAGTATATCCCCCTCTTTCT AGAAATATTGCCCATGCAGGAGCTTCTGTTGATTTAGCAATTTTTTCTCT TCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTGTTAATTTTATTACTA CAGTTATTAATATGCGATCTACTGGAATTACATTTGATCGTATACCACTT TTTGTATGATCTGTAGTTATTACAGCCGTTTTACTTCTCCTATCCCTCCC AGTTTTAGCCGGGGCTATTACTATACTATTAACAGATCGAAATTTAAATA CAAGTTTCTTTGACCCAGCAGGAGGGGGAGACCCTATTTTATACCAACAT TTATTTTGATTTTTTGGT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_8407-scaled.jpg,",,,08/27/2024,"
\n
Environment: Dry Grassy Area, Very Warm Climate<\/li>\n
Location: West of Jenkins Hall on Georgia Southern University – Armstrong Campus<\/li>\n
Time of Collection: 1:05 PM<\/li>\n<\/ul>\n",Diptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-7.png,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,GAACTTCACTAAGTATATTAATTCGAACAGAATTaacccttccAGGATTT TTTATTGGTGATGATCAAATTTATAATGtcaTTGTTACAGCCCATGCTTT TATTATAATTTTTTTTATAGTTATACCAATTATAATTGGTGGATTTGGAA ACTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTTCCTCGA ATAAATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTCTTCT AATAAGAAGCATAGTTGAAAACGGGGCTGGTACTGGATGAACAGTATATC CCCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTTAGCA ATTTTTTCTCTTCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTGTTAA TTTTATTACTACAGTTATTAATATGCGATCTACTGGAATTACATTTGATC GTATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACTTCTC CTATCCCTCCCAGTTTTAGCCGGGGCTATTACTATACTATTAACAGATCG AAATTTAAATACAAGTTTCTTTGACCCAGCaggaggggGAGACCCTATTT TATACCAACATTTATTTTGATTTTTTGGTCACCCTGAAGTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3630-scaled.jpeg,",31.9708128000,-81.1586515000,08/26/2024,"
\n
Method and Location- <\/span>\u200b<\/li>\n<\/ul>\n
Beating Cloth Method<\/span>\u200b<\/p>\n
Beat bush with cloth placed under it<\/span>\u200b<\/p>\n
Next to the offices outside of <\/span>science center<\/span>\u200b<\/p>\n
\n
\u200b<\/li>\n
Time and Conditions- <\/span>\u200b<\/li>\n<\/ul>\n
8\/26\/24<\/span>\u200b<\/p>\n
1:40 pm.<\/span>\u200b<\/p>\n
\u00a0Cloudy<\/span>\u200b<\/p>\n",Coleoptera,,body without wings,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Picture1_VSCO.jpeg,","
DNA extraction kit of in-house reagents was used<\/p>\n",No,TTTCCTATTTggtaGATGAGCAGGaataGTAGGAACCTCCCTTAGACTAC TAATTCGaGCAGAACTCGGAAATCCTGGATCTCTGATTGGAGACGACCAA ATTTACAATGTTATTGTTACTGCACATGCTTTCATCATAATTTTTTTCAT GGTAATACCAATTATAATTGGTGGATTCGGAAACTGGCTTGTCCCTCTAA TACTTGGGGCCCCTGACATAGCTTTCCCCCGAATAAATAACATAAGATTT TGGCTTCTTCCTCCTTCTTTAACTTTACTTTTAATGAGAAGATTAGTAGA AAGAGGAGCTGGAACTGGGTGAACAGTATACCCCCCTTTATCTAGAAACA TCGCTCACAGAGGGGCTTCTGTAGATCTAGCTATTTTTAGTTTACACCTA GCTGGAATTTCATCTATTCTTGGAGCTGTAAATTTCATTACTACAGTTAT TAACATACGtTCTACAGGCATAACATTTGACCGAATACCATTATTTGTAT GATCagtagctTTAACaacTCTTTTACTACTTCTGTCCCTGCCTGTCCTA GCTGGCGCAATCACGATACTTTTAACTGATCGAAATATTAATACCTCATT CTTTGACCCAGCGGGTGGTGGAGACCCAATTTTATACCAACACTTATTTT GATTTTTTGGT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/image-scaled.jpg,",31.9816141443,-81.1588760798,08/27/2024,"
I fought my bug in the tall grass area near the trees at the GSU Armstrong campus. We used the sweep net nothin to collect the insects. It was mildly hot outside.<\/p>\n",Coleoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_1051.jpeg,","
DNA extraction kit of in-house reagents was used<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_6529-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_5148.jpeg,",31.9708128,-81.15865149999999,08/27/2024,"
Around 10 am, humid and warm outside, in the long grass behind the science center building, used sweep method to catch<\/p>\n",Hemiptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Gel-Image-scaled.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,TAtgAGCAGGTATAGTAGGTTCCGCCATAAGAATCATCATTCGTATTGAA CTAGGACAACCAGGAAGATTTAttggggGATGACCAAATCTATAATGTAG TAGTCACCGCTCACGCATTCGTAATAATTTTCTTTATAGTAATACCAATT ATAATTGGGGGTTTTGGTAACTGATTAGTACCATTAATAATTGGAGCCCC TGATATAGCATTTCCTCGTATAAATAATATAAGATTCTGACTATTACCCC CTTCAATTACATTATTAATAATAAGTAGATTGGCTGAATCAGGAGCAGGG ACTGGTTGAACCGTCTACCCCCCATTATCTAGTAATCTATCACACAGAGG AGCATCAGTAGACTTAGCTATTTTTTCTCTACATCTAGCAGGAGTATCCT CAATCCTAGGAGCTGTAAATTTCATCTCAACAATCATTAATATACGCCCA GAAGGAATAATTCCAGAACGAATTCCCTTATTTGTTTGATCAGTAGGGAT CACCGCTCTTCTTCTTCTTCTATCACTTCCTGTATTAGCAGGAGCTATCA CCATACTATTAACTGACCGAAATTTTAATACTTCCTTCTTTGACCCTTCA GGAGGGGGAGACCCTATTTTATATCAACATTTATTTTGATTTTTTGGT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0534-scaled.jpg,",40.875891,-81.4023356,10/06/2024,"",Arthropoda,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_5396.jpeg,",31.9708128000,-81.1586515000,08/27/2024,"
Field by the retention pond<\/p>\n
GA Southern Armstrong Campus<\/p>\n
warm weather post-rain<\/p>\n
sweep net, forceps, mason jar<\/p>\n",Diptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-05-at-11.47.48 AM.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,TtngGaACTTCACTAAGTATATTAATTCGAACAGAATTAAGCCATCCAGG ATTTTTTATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCCCATG CTTTTATTATAATTTTTTTTATAGTTATACCAATTATAATTGGTGGATTT GGAAACTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTTCC TCGAATAAATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTC TTCTAATAAGAAGCATAGTTGAAAACGGGGCTGGTACTGGATGAACAGTA TATCCCCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTT AGCAATTTTTTCTCTTCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTG TTAATTTTATTACTACAGTTATTAATATGCGATCTACTGGAATTACATTT GATCGTATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACT TCTCCTATCCCTCCCAGTTTTAGCCGGGGCTATTACTATACTATTAACAG ATCGAAATTTAAATACAAGTTTCTTTGACCCAGCAGGAGGGGGAGACCCT ATTTTATACCAACATTTATTTTGATTTTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_2069-scaled.jpg,",31.9708128000,-81.1586515000,08/27/2024,"
10:10 am, humid and sunny morning, caught in the high grass on the far east side of the student parking lot, caught by using the sweeping method.<\/p>\n",Hemiptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_2534-copy-1.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0221-2.jpg,",,,08/27/2024,"
Hot, Humid, Grassy Area<\/p>\n",Orthoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Reagan-and-Catherine_new.jpg,","
DNA extraction kit of in-house reagents was used<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-04-at-7.52.54 PM.png,",31.9811344227,-81.1583139316,08/27/2024,"
Location of Collection:<\/b> Georgia Southern\u2019s aquaponics greenhouse. Savannah, Georgia, 31419<\/span><\/p>\n
Habitat:<\/b> The edges of long grass, near a water source. Not far from a walking path.<\/span><\/p>\n
Method of Collection<\/b>: My partner and I collected our sample using a Sweep Net and swept the blades of grass for insects<\/span><\/p>\n",Hemiptera,,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Untitled.png,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/PXL_20241007_151906805.MP_-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/PXL_20241007_151135694.MP_-scaled.jpg,",41.1066744748206,-81.24864298561437,10/03/2024,"
Caught Flying around with a bunch of other March flys<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0085-scaled.jpeg,",31.9708128,-81.15865149999999,,"
\n
Date: 2\/26\/2024<\/span>\u200b<\/li>\n
Time: 1:47 p.m.<\/span>\u200b<\/li>\n
Location: <\/span>\u200b<\/li>\n
Front of Lane Library Bushes. <\/span>\u200b<\/li>\n
Compass Plaza Bushes<\/span>\u200b<\/li>\n
Flowers on the side of Learning Commons<\/span>\u200b<\/li>\n
Behind Library Hydrangea Plant<\/span>\u200b<\/li>\n
Environment: Sunny<\/span>\u200b<\/li>\n
Method: Sweeping Method<\/span><\/li>\n<\/ul>\n",Lepidoptera,,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0774-scaled.jpeg,","
DNA extraction kit of in-house reagents was used.<\/p>\n",No,>01D_GREAA-MT_CO1F TRIM QUALITY: 20 TACTTCTTTAAGTTTATTAATTCGAACAGAATTagccatcccAGGATCTT TAATTGGAGATGATCAAATTTATAATACTATTGTTACAGCTCATGCTTTT ATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGTAA TTGATTAGTTCCTTTAATATTAGGGGCTCCTGATATAGCTTTTCCTCGTA TAAATAATATAAGATTTTGGATACTTCCTCCTTCTCTAACTTTATTAATT TCAAGAAGTATTGTAGAAAATGGAGCAGGAACAGGTTGAACAGTTTATCC TCCTCTTTCTTCTAATATTGCTCATCAAGGAGCTTCTGTTGATTTAGCAA TTTTTTCTCTTCATTTAGCAGGTATTTCATCTATTTTAGGAGCTATTAAT TTTATTACTACAATTATTAATATACGAATTAAAAATTTATCATTTGATCA AATACCTTTATTTGTTTGATCTGTTGGTATTACAGCCTTATTATTACTTT TATCTTTACCAGTTTTAGCAGGAGCTATTACTATACTTTTAACTGATCGA AATTTAAATACTTCATTTTTTGATCCTGCTGGAGGAGGAGATCCAATTTT ATATCAACATTTATTTTGATTTTTTGGTCACC,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3622-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_3623-scaled.jpeg,",42.1168098906,-76.2726557678,09/06/2024,"
The Ants looked like normal ants, they were found in a grassy area, with some rocks.<\/span><\/p>\n",Hymenoptera,DNeasy (Qiagen),Rear half,MiniOne,,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0755.jpeg,","
When our group had put the comb into the tray that had our melted gel in it, the comb wasn\u2019t even within the tray. This resulted in our gel coming out with uneven wells throughout the gel.<\/span><\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0648.jpeg,",42.1165282065,-76.2740756839,09/10/2024,"
Moths and grasshoppers alike are large arthropods with thick exoskeletons that should contain large amounts of DNA<\/p>\n",Arthropoda,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0645.jpeg,","
In gel 2, lanes 4 and 5 had a small hole about half way down the gel, but this did not effect the results in any way<\/p>\n",No,detected in all samples except water,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_0836.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_0835.jpeg,",42.1034075000,-76.2621549000,09/06/2024,"
The ant\u2019s we collected were different sizes but from the same location. They were on a sidewalk near some mulch and grass.<\/p>\n",Hymenoptera,DNeasy (Qiagen),,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_0839-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_0840-1.jpeg,","
In Figure 1, lane one, a DNA Ladder is shown. A DNA Ladder is a \u201ccocktail\u201d of fragments of DNA. The ladder itself is used as a reference tool to estimate the band size, and to make sure the gel electrophoresis test runs properly. Lanes two and three contain arthropods one and two: the ants that were used to test for Wolbachia. In lane four, the positive arthropod control is shown. This gives a group in which it is guaranteed that there is arthropod DNA present. Lane five should contain arthropod DNA, but should not contain Wolbachia DNA. Unfortunately, an error occurred within the process of getting the arthropod into the tube, causing there to be no arthropod DNA in this tube whatsoever. Due to this, there is not a band, meaning one of our controls did not work, as it did not exist. Lane six shows positive DNA control, meaning there is guaranteed DNA in there. Lane seven is water, and it was used to ensure there was no contamination in the experiment.<\/p>\n
In figure 2, the DNA ladder still exists in lane one, therefore the gel electrophoresis ran properly. The positive DNA control in lane four remains the same, as there is both arthropod and Wolbachia DNA present. In lane five, there is again no band present where the negative arthropod control should be, which is an error, as this control should contain Wolbachia DNA, but not arthropod DNA. In lane seven water, the water band remains the same. Arthropods one and two have no bands. This means that there was no Wolbachia present in the arthropods that were examined.<\/p>\n",No,,,, "",36.2251261493,-86.8619143274,11/20/2024,"",Chrysochraon,pcr,Abdomen,MiniPCR,Other,,"","",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/42_Arthropod-CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/1-scaled.jpg,",38.9293471122,-77.0697727257,09/03/2024,"
I found this pill bug in a rotting log by a trail path in the Olmstead Woods in the morning. There were other pill bugs in the same area. This pill bug is a little bit smaller than most pill bugs I’ve seen.<\/p>\n",Armadillidium nasatum,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolbachia_Gel.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/COI_Gel.png,","",Yes,CGCTTTAAGAATAATTATTCGGACTGAATTAGGGCAGCCTGGGAGTCTCATTGGGGATGATCAAATCTATAATGTAATTGTCACGGCTCATGCTTTTGTAATGATTTTCTTCATAGTAATACCTATTATAATTGGAGGTTTTGGAAATTGATTAATTCCTTTGATATTAGGGGCTCCTGATATAGCTTTCCCTCGTATAAACAATATAAGATTTTGGCTGCTTCCCCCTTCTTTAACTTTACTCCTTAGCAGAGGATTAATTGAAAGAGGAGTAGGAACGGGATGAACTGTTTACCCTCCTTTGGCATCAAGAATTGCCCATAGTGGAGCTTCTGTAGATTTAGGTATCTTTTCTCTTCATCTTGCTGGGGCTTCATCAATTTTAGGGGCAGTAAACTTTATTACGACTATAATTAATATACGGGCGTCCGGAATTAGTATGGATCGTGTGCCTTTATTTGTTTGGTCTGTTTTAGTAACCGCGGTTCTTCTTCTCTTATCTTTACCTGTTCTTGCTGGAGCTATTACTATACTATTGACTGACCGTAATTTAA,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/1-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-02-185430.png,",41.1067447882,-81.2484672237,10/03/2024,"
It was on a leaf and was very active.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/1000000367-scaled.jpg,",41.0992125000,-81.2425834000,10/03/2024,"
We found her buzzing near a flower. She was very dulcet and wasn’t flying around much just relaxing on a petal.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-01-090251.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-01-090241.png,",41.10554101584366,-81.24394201084586,10/08/2024,"
The Crane Fly was found in a high grass area perching in the grass. The Crane fly was very active.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/6-scaled.jpg,",39.0124074272,-77.1109313911,09/04/2024,"
Caught in a house, at room temperature. Arthropod was found in web in the corner of my house.<\/p>\n",Araneae,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Screenshot-2024-10-31-175022.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Screenshot-2024-10-31-175028.png,","",No,GTAGATTTATGGGTGATGATCAATTATATAATGTAATTGTAACGGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATACCAATTTTAATTGGAGGGTTTGGAAATTGATTAGTTCCATTAATATTAGGGGCACCTGACATAGCATTTCCTCGAATAAATAATTTGAGATTTTGATTATTACCCCCTTCTTTATTATTATTATTTATTTCGTCTATAGTAGAAATAGGAGTAGGAGCTGGGTGAACTGTATATCCACCTCTATCATCTTTAGATGGTCACGGTGGTAGATCAGTAGATTTTGCAATTTTTTCTTTGCATTTAGCTGGGGCTTCTTCTATTATAGGTGCGGTAAATTTTATTTCTACTGTACTCAATATGCGATCTTATGGGATGAGAATAGAAAAAGTTCCTTTATTTGTATGATCAGTTCTAATTACTGCTGTGCTATTATTATTATCATTACCAGTATTAGCAGGTGCTATTACTATATTATTAACTGATCGAAATTTTAATACTTCATTTTT,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/6-CO1F-1-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/NIU-9.jpg,",41.9450980000,-88.7554033000,08/06/2024,"
wasp mimic<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/NIU-8.jpg,",41.945098,-88.7554033,08/06/2024,"
bald faced hornet<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/NIU-7--scaled.jpg,",41.9315471,-88.7636445,09/19/2024,"
yellow jacket<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/NIU-6-1.jpg,",41.9315202,-88.7636323,09/19/2024,"
fruit fly<\/p>\n",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_0634.jpeg,",42.118192004510256,-76.27751670946265,09/06/2024,"
Collected on the shore of the Owego Creek<\/p>\n",Coleoptera,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_0635.jpeg,","
The Wolbachia gel electrophoresis protocol depicted in Figure. 1 shows no bands in any other column besides the DNA ladder.\u00a0 There could be many explanations to why this part of the project failed, but a possibility could be that there was not enough DNA control left so we may have not received an accurate measurement of control affecting our results.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Honey-Bee-1.png,",41.1065108316,-81.2483315629,10/06/2024,"
Bumblebee was found in the tall grass next to flowers.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_2303.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_2304.jpeg,",38.9425353000,-77.0615313000,09/17/2024,"
Caught in science lab in late summer. Spotted, orangeish color, slightly damaged wing.<\/p>\n",Coccinellidae,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_2301-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_2302-1.jpeg,","
DNA Extraction: Arthropod was crushed easily and there were no issues throughout the extraction process.<\/p>\n
PCR: No mistakes throughout process.<\/p>\n
Gel electrophoresis, Arthropod CO1: Well C2 was the Coleoptera, W+ and W- were positive and negative for Wolbachia but still contained arthropod DNA, and H2O is water to be a control for contamination. C2 had a band between 800 and 600 base pairs, same with W+ and W-. Water had no band so there is no reason to assume the samples were crosscontaminated.<\/p>\n
Gel electrophoresis,\u00a0Wolbachia<\/em>: There was no band present for the C-2 lane indicating it was not positive for\u00a0Wolbachia<\/em>. We have no reason the believe this is false because the positive\u00a0Wolbachia<\/em> control and a band and the negative\u00a0Wolbachia<\/em> control and the the water control did not have bands.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_2297-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_2298-1.jpeg,",38.9425353000,-77.0615313000,09/10/2024,"
Caught by in an office space during late summer, 70\u00ba. Pale yellow, all 8 legs intact, body less than an inch in length. \u00a0Urban habitat.<\/p>\n",Arachnida,DNeasy (Qiagen),Whole arthropod,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_2301.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_2302.jpeg,","
PCR:<\/p>\n
No known mistakes made, protocol followed.<\/p>\n
Gel electrophoresis, Arthropod CO1:<\/p>\n
C1 was the Aracneae , C2 and C3 were other arthropods. W+ was positive for Wolbachia, W- was negative for Wolbachia but still contained arthropod DNA. Water was the control and no band appeared, confirming that the samples were not contaminated with other DNA.<\/p>\n
Gel electrophoresis,\u00a0Wolbachia<\/em>:<\/p>\n
The Aracneae was C1, C2 was another sample, and so was C3. W+ was the only lane with a band because it was the positive control. W- had no band as it was the negative control. Water also had no band which confirmed no contamination.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_4054.jpeg,",38.8989958051,282.9605687622,09/06/2024,"
Collected during summer, found on stone wall by house, it was 69 degrees Fahrenheit outside, urban habitat, 6 legs, no wings, short brown antenna, black eyes.<\/p>\n",Orthoptera,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_4051.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_4047.jpeg,","
DNA Extraction:<\/p>\n
Arthropod was easy to extract DNA from, abdomen was directed and goop was scooped into the tube<\/p>\n
PCR: No mistakes made, but Had to spin 3 times in centrifuge, had too much liquid left at top after first and second spins. <\/span>Had to spin 2 times in centrifuge after buffer AW1 was added<\/span><\/p>\n
Gel Electrophoresis, Arthropod CO1:<\/p>\n
The first lane of our Gel #1 contained our DNA ladder, the second lane contained our A4 sample (Spider Cricket), third lane had A5 sample (Common Moth), fourth lane had sample A6 (Brown Millipede), Lanes 5 through seven contained our positive arthropod control, our negative arthropod control and our H2O control.<\/p>\n
Our controls worked so our results are valid.<\/p>\n
Gel Electrophoresis, Wolbachia:<\/p>\n
In our first gel, we had in lane one our DNA ladder, lane 2 had sample A4 (Spider Cricket), the third lane had sample A5 (Common Moth), our fourth lane had sample A6 (Brown Millipede), and then in lanes 5 through 7 had our positive Wolbachia control, negative Wolbachia control, and our H2O control. Our controls worked which means we experienced no contamination and our results are valid.<\/p>\n
<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_4043.jpeg,",38.9425261096,-77.0580138050,09/06/2024,"
Collected during summer, found on the sidewalk in front of house, it was 69 degrees Fahrenheit outside, urban habitat, the arthropod is bright green, has one body segment, black and white antennae, and is and one centimeter long.<\/p>\n",Hemiptera,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"","
DNA Extraction:<\/p>\n
Arthropod was difficult to crush up as the outer hard shell was still attached.<\/p>\n
PCR: No mistakes made<\/p>\n
Gel Electrophoresis, Arthropod CO1:<\/p>\n
The first lane of our Gel #1 contained our DNA ladder, the second lane contained our A1 sample (Luna Moth), third lane had A2 sample (European Hornet), fourth lane had sample A3 (Green Stink Bug), Lanes 5 through seven contained our positive arthropod control, our negative arthropod control and our H2O control.<\/p>\n
Our controls worked so our results are valid.<\/p>\n
Gel Electrophoresis, Wolbachia:<\/p>\n
In our first gel, we had in lane one our DNA ladder, lane 2 had sample A1 (Luna Moth), the third lane had sample A2 (European Hornet), our fourth lane had sample A3 (Green Stink Bug), and then in lanes 5 through 7 had our positive Wolbachia control, negative Wolbachia control, and our H2O control. Our controls worked which means we experienced no contamination and our results are valid.<\/p>\n
<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-07-at-11.15.51-AM.png,",38.9071923000,-77.0368707000,09/24/2024,"
\n
\n
\n
\n
The\u00a0Cricket<\/em> has a yellow body with a shiny shell with marks. It has long, thin antennae, large back legs, and segmented legs and body. The eyes are prominent, and its snout has small appendages. The cricket was caught in a semi-urban park with lush pastures where the bug resides contrasting heavily with the loud urban backdrop. The season was fall and it was caught at night with a temp of about 72 degrees Fahrenheit.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
\n
\n
\n
<\/p>\n
<\/div>\n<\/div>\n<\/div>\n<\/div>\n",Tachycines asynamorus,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Screenshot-2024-10-30-at-10.13.39-AM-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-07-at-11.26.58-AM.png,","
<\/p>\n
DNA Extraction<\/span><\/p>\n
\n
The DNA extraction went well for the most part. The only issues of note are that we added about 50ul of extra Buffer to B-2, B-3, and B-5. We also spilled a little bit of B-5.<\/span><\/li>\n<\/ol>\n
PCR\u201d:<\/span><\/p>\n
\n
There was one notable mistake during the PCR, we forgot to switch pipette tips between samples possibly contaminating them.<\/span><\/li>\n<\/ol>\n
Gel electrophoresis, Arthropod CO1<\/span><\/p>\n
\n
The ladder was marked on all Gels<\/span><\/li>\n
Samples B-1 through B-6 were positive for arthropod DNA\u00a0<\/span><\/li>\n
The positive and negative controls were also marked positive and water was marked negative<\/span><\/li>\n<\/ol>\n
<\/p>\n
Gel electrophoresis, <\/span>Wolbachia<\/span><\/i><\/p>\n
\n
B-1 through B-5 were negative and B-6 was positive<\/span><\/li>\n
The controls worked with both negative and water controls marked negative and the positive control marked positive<\/span><\/li>\n
There were no notable mistakes or errors<\/span><\/li>\n<\/ol>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Screenshot-2024-10-30-at-2.54.20-PM.png,",38.9071923000,-77.0368707000,09/18/2024,"
small head and a broad, flattened body, reddish-brown, large compound eyes, two ocelli, and long, flexible antennae. The arthropod was caught outside of a manhole at night in an urban area. The season was fall and the temperature was about 76 degrees.<\/p>\n",Blattodea,DNeasy (Qiagen),Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Screenshot-2024-10-30-at-3.06.31-PM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-07-at-11.42.27-AM.png,","
<\/p>\n
DNA Extraction<\/span><\/p>\n
\n
The DNA extraction went well for the most part. The only issues of note are that we added about 50ul of extra Buffer to B-2, B-3, and B-5. We also spilled a little bit of B-5.<\/span><\/li>\n<\/ol>\n
PCR\u201d:<\/span><\/p>\n
\n
There was one notable mistake during the PCR, we forgot to switch pipette tips between samples possibly contaminating them.<\/span><\/li>\n<\/ol>\n
Gel electrophoresis, Arthropod CO1<\/span><\/p>\n
\n
The ladder was marked on all Gels<\/span><\/li>\n
Samples B-1 through B-6 were positive for arthropod DNA\u00a0<\/span><\/li>\n
The positive and negative controls were also marked positive and water was marked negative<\/span><\/li>\n<\/ol>\n
<\/p>\n
Gel electrophoresis, <\/span>Wolbachia<\/span><\/i><\/p>\n
\n
B-1 through B-5 were negative and B-6 was positive<\/span><\/li>\n
The controls worked with both negative and water controls marked negative and the positive control marked positive<\/span><\/li>\n
There were no notable mistakes or errors<\/span><\/li>\n<\/ol>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/unnamed-1-1.png,",41.1048854,-81.2440474,10/03/2024,"
The honey bee was active and flying around various species of plants and flowers.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/4-scaled.jpg,",39.0109360746,-77.0872615070,09/04/2024,"
The mosquito was found in a leafy area near an oak tree in Rock Creek Park.<\/p>\n",Culicidae,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/gels.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/gels-2.jpg,","
DNA extraction<\/strong>: I made sure to crush the arthropod very well. Then, I dissected the abdomen and did not include the exoskeleton.<\/p>\n
All of the lanes are labeled on the gel image<\/p>\n
Lanes 9 and 10 are reruns of an originally unsuccessful attempt for a classmate’s Pill Bug<\/p>\n
The Wolbachia bands were 438bp each<\/p>\n
The CO1 bands were 708bp each<\/p>\n
On the CO1 gel, my arthropod is labeled, along with the positive CO1 control and the 100bp ladder.<\/p>\n",Yes,CTGGGAGTCTCATTGGGGATGATCAAATCTATAATGTAATTGTCACGGCTCATGCTTTTGnAATGATTTTCTTCATAGTAATACCTATTATAATTGGANGTTTTGGAAATTGATTAATTCCNTTGATATTAGGGGCTCCTGATATAGCTTTCNCTCGTATAAACAATATAAGATTTTGGNTGNTTCCCCCNTCTTTAACNTTACTCCTTANCAGANGATTANTTGAAANAGGAGTAGGAACGGGATGAACTGTTTACCCTCCTTTGGCNTCNNNAATTGCCCATNNTGGAGCTTCTGTAGATTTAGGTATCTTTTNTCTTCATCTTGCTGGGGCTTCATCAATTTTAGGGGNAGTAAACTTTATTACNACTNTNATTAATATACGNGCNTCCGGAATTACTATGGATCGTGTGCCTTTATTTGTTTGNTCTGTTTTANTAACCGCGGTTCTTCTTCTCTTATCTTTACCTGNT,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/4-CO1F.fa,, "",35.8601190000,-86.6601560000,11/29/2024,"",Arthropoda,,,,,,"","",,,,, "",,,,"",,,,,,,"","",,,,, "",,,,"",,,,,,,"","",,,,, "",,,,"",,,,,,,"","",,,,, "",,,,"",,,,,,,"","",,,,, "",,,,"",,,,,,,"","",,,,, "",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2026/01/Red-legged-orb-weaver-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2026/01/Red-legged-orb-weaver-2.jpg,",,,10/30/2024,"
Arachnid was found in the back of my yard on a leaf. The arachnid was found and proven to be female based on closer examination as well.<\/p>\n",Araneidae,DNeasy (Qiagen),Abdomen,agarose gel electrophoresis,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2026/01/IMG_20241212_140345479-scaled.jpg,","
PCR notes:<\/p>\n
Purpose: To extend the DNA samples that we retrieved by increasing the amount of DNA by producing copies of the DNA. <\/span><\/p>\n
Materials:<\/span><\/p>\n
\n
Thermal cycler<\/span><\/li>\n
Vortex<\/span><\/li>\n
Mini-centrifuge<\/span><\/li>\n
Gloves<\/span><\/li>\n
Squirt bottle<\/span><\/li>\n
0.2 mL PCR tubes<\/span><\/li>\n
Rack for 0.2 mL PCR tubes<\/span><\/li>\n
1.5 mL tubes<\/span><\/li>\n
Rack for 1.5 mL<\/span><\/li>\n
Balance tube<\/span><\/li>\n
Disposal cup for tips<\/span><\/li>\n
Pipettes (200\u00b5L and 20\u00b5L)<\/span><\/li>\n
Sharpie<\/span><\/li>\n
DNA samples from arthropod specimens<\/span><\/li>\n
+\/- Wolbachia controls<\/span><\/li>\n
+DNA control<\/span><\/li>\n
Sterile, nuclease-free water<\/span><\/li>\n
Taq Master Mix<\/span><\/li>\n
Wolbachia_F and Wolbachia_R primers<\/span><\/li>\n<\/ul>\n
Methods:<\/span><\/p>\n
\n
A teacher had prepared the Thermal cycler and PCR program was entered<\/span><\/li>\n
All unnecessary items were removed from lab<\/span><\/li>\n
Nitrile gloves were put on and all surfaces were wiped down with 70% Ethanol.<\/span><\/li>\n
1.5 mL microcentrifuge tubes were collected. Tube was labeled with “PCR” tube was placed on a 1.5mL tube rack<\/span><\/li>\n
Eight 0.2 mL PCR tubes were collected. Tubes were labeled and numbered with initials. Tubes placed in PCR tube rack.<\/span><\/li>\n
P-20 pipette were used and 2\u00b5L of template DNA were added to corresponding PCR tube. Tips were changed between each tube.<\/span><\/li>\n
PCR tubes were set aside. Templated DNA tubes were placed into storage<\/span><\/li>\n
P-200 pipette were used and reagents from the 9 reactions were added to the 1.5mL tube marked “PCR”. Tips were changed between each reagent and reagent were checked off after it was added.<\/span><\/li>\n
Tube was closed and briefly vortexed for 5 seconds<\/span><\/li>\n
PCR tube was placed on one side of the mini-centrifuge and Balancer tube on opposite sides. PCR Cocktail was quickly spun down for 4 seconds.<\/span><\/li>\n
P-200 pipette was used and 23 \u00b5L of PCR was added to tubes 1-8. Tips were changed between each tube. <\/span><\/li>\n
Lids were secured on each tube<\/span><\/li>\n
PCR tube rotor was used on mini-centrifuge. Tubes 1-4 were centered on one side and 5-8 on the other. Liquids were briefly spun to collect liquid<\/span><\/li>\n
Tubes were transferred to thermal cycler. <\/span><\/li>\n
Lab station was cleaned and surfaces were wiped with ethanol.<\/span><\/li>\n<\/ol>\n
Gel Electrophoresis:<\/p>\n
Purpose: <\/b>To create a agarose gel that allows us to determine whether or not our samples contain Wolbachia<\/i>. By<\/p>\n
analyzing the distance the DNA strand traveled.<\/p>\n
Materials:<\/b><\/p>\n
\n
Electrophoresis tank<\/li>\n
Power supply<\/li>\n
Standard Electrophoresis casting tray and comb<\/li>\n
Microwave<\/li>\n
Imager<\/li>\n
Electronic balance<\/li>\n
Squirt bottle<\/li>\n
Gloves<\/li>\n
Oven mitt<\/li>\n
Eye protection<\/li>\n
Weigh Dish<\/li>\n
Running Buffer TAE<\/li>\n
250mL Erlenmeyer flask<\/li>\n
Graduated cylinder<\/li>\n
Rack<\/li>\n
0.2mL PCR tubes<\/li>\n
20 microliter pipette<\/li>\n
20 microliter pipette tips<\/li>\n
Waste cup<\/li>\n
Sharpie<\/li>\n
Agarose powder<\/li>\n
DNA stain<\/li>\n
PCR products<\/li>\n
DNA ladder<\/li>\n<\/ul>\n
Methods:<\/b><\/p>\n
\n
As class, 1 working solution of electrophoresis running buffer (TAE) was prepared<\/li>\n
All unnecessary equipment was removed from lab.<\/li>\n
Nitrile gloves were put on and all surfaces were cleaned with 70% ethanol<\/li>\n<\/ol>\n
Prepare the Gel with DNA Stain:<\/b><\/p>\n
\n
0.5g were measured and added to 250 mL flask<\/li>\n
50 mL of running buffer were added to 250mL flask<\/li>\n
Parafilm was initially used for the microwaving (film melted after 60 seconds)<\/li>\n
\u00a0Material was switched to Kimwipe for a 30 second interval excess film was cleaned off. The agarose was melted into Buffer\/Solvent<\/li>\n
The solution was cooled to about 50-55C, flask was swirled occasion until it was warm to touch.<\/li>\n
Casting tray ends were sealed by placing it in to the gel electrophoresis tank<\/li>\n
10 microliters were added to agarose. Was swirled to mix<\/li>\n
Comb was selected that accommodated all samples. Combs were placed in gel casting tray<\/li>\n
The melted agarose was slowly poured into the casting tray.<\/li>\n
Gel was allowed to cool undisturbed on a solid, flat surface for 20 minute\u00a0 s until it was solid<\/li>\n
Combs were carefully pulled out and the casting tray was lifted out of the electrophoresis chamber.<\/li>\n
Gel was placed in the electrophoresis chamber with well oriented near the negative electrode.<\/li>\n
Running buffer was estimated and added over the gel.<\/li>\n<\/ol>\n
Prepare to load the Gel:<\/b><\/p>\n
\n
<\/b>Loading Key was fill out on the next page<\/li>\n
2\u00b5L drops of 5X loading buffer were pipetted onto a piece of Parafilm. 10\u00b5L were added from each PCR reaction of loading buffer. Mixed well by pipetting up and down several times until the color of the liquid is homogenous.<\/li>\n
10\u00b5L of the DNA ladder was pipetted into the first well. Pipette was hovered above well, pipette was slowly emptied.<\/li>\n
Pipetted carefully 10\u00b5L of each PCR sample in different wells. Tips were changed between each sample.<\/li>\n<\/ol>\n
Run the Gel:<\/b><\/p>\n
\n
Lid was placed on the gel box, electrodes were connected appropriately.<\/li>\n
Power supply was turned to 125 volts.<\/li>\n
Current was checked to make sure it ran through buffer<\/li>\n
Current was checked to be moving in the correct direction.<\/li>\n
Power ran until the yellow was 3\/4 down the gel. Power was then turned off and electrodes were disconnected<\/li>\n<\/ol>\n
lid was removed along with gel using gloves<\/p>\n
Obtain an image of the Gel:<\/b><\/p>\n
\n
Gel was placed on the transilluminator. Eye protection was put on.<\/li>\n
Prescence and absence of bands were noted in each lane.<\/li>\n
Results were documented in lab notebook and a picture of gel was taken<\/li>\n
Used tips were discarded and bench was wiped down.<\/li>\n
Gel images were transferred to computer.<\/li>\n<\/ol>\n
<\/p>\n",Unknown,,,, "",36.0792242,-86.7686604,11/05/2024,"",,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/tempImaget3oxCX-scaled.jpg,",36.0800286476,-86.7697988307,11/01/2024,"
Found outside.<\/p>\n",Arthropoda,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"","",,,,, "",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/thumbnail_IMG_8749-rotated.jpg,",36.0601814572,-86.7617844256,10/31/2024,"
Specimen was found under a flat rock with other crickets of the same species.<\/p>\n",Gryllus pennsylvanicus,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Gel.jpg,","
During PCR, a pipette tip was used to distribute PCR cocktail to multiple samples without being changed in between. This means that the samples may be cross-contaminated, but the controls show no evidence of this.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/insect-1.jpg,",36.0751300000,-86.7111952000,10/22/2024,"
Arthropod was collected in the backyard of a house. The backyard was grassy, had many plants growing and grown, had pear & apple trees, and the soil was a little dry.<\/p>\n",Arthropoda,DNeasy (Qiagen),Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2025/12/Wolbachia_GelElectrophoresis_Results.png,","
Before being placed under UV light, the gel containing the DNA of all arthropods cracked on the ground. This means that the results of these samples may be inaccurate due to contamination and leakage of DNA.<\/p>\n
<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_20241007_110917230-rotated.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_20241007_112203139-scaled.jpg,",41.1069492510,-81.2470872368,10/03/2024,"
Found on a Plant<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Honeybee.jpg,",41.1046397000,-81.2444114000,10/03/2024,"
Bee was captured off of a flower in the outdoor lab garden, was active for a little while after capture.<\/p>\n",Apidae,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-08-122007-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-08-122000.png,",41.1062850000,-81.2488149000,10/03/2024,"
Cricket was found jumping around in the field.<\/p>\n",Conocephalus melaenus,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Screen-Shot-2024-10-29-at-11.30.24-AM.png,",41.1046397000,-81.2444114000,10/03/2024,"
Found in long-grass, very active. Climbed on me.<\/p>\n",Melanoplus differentialis,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot-2024-11-08-121959.png,",41.1067331870,-81.2487264516,10/06/2024,"
Crane fly was found in wet grass and caught while it was flying.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,,,,,"","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_3406-1-scaled.jpg,",41.10600385785052,-81.24658120755039,10/03/2024,"
The spider was found moving in grass, very active.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_4261-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_4263-scaled.jpeg,",41.10463393472847,-81.24452597342355,10/03/2024,"
Was very active when caught<\/p>\n
found in grass<\/p>\n",Arthropoda,DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Screenshot-2024-10-29-11.17.10-AM-1.png,",41.105990546171626,-81.24686860343373,10/03/2024,"
Butterfly was flying in the air, and then stopped and rested on the ground.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/AsianLadyBeetle1.png,",41.10653341122719,-81.24829739872402,10/03/2024,"
Lady Asian Beetle was found on a flower, very active.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_3337-1.jpg,",40.8758910000,-81.4023356000,10/06/2024,"
Found on the seedhead of a swamp milkweed plant behind a home.<\/p>\n",Oncopeltus fasciatus,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Opera-Snapshot_2024-10-29_110901_docs.google.com_.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Opera-Snapshot_2024-10-29_110950_docs.google.com_.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Opera-Snapshot_2024-10-29_110933_docs.google.com_.png,",41.0976256000,-81.2417024000,10/03/2024,"
Honey Bee was caught flying from a flower<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/8-1-scaled.jpg,",38.9310299099,-77.0682871070,08/28/2024,"
Captured in the corner of a room in a building during the summer.<\/p>\n",Pholcus crypticolens,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolbachia-Gel-Labeling-2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Gel-Labeling-1-1.jpg,","",Yes,TTTAATATTAGGAGCTCCAGATATAGCGTTTCCTCGGATAAATAACTTGAGATTTTGGTTATTACCTCCTGCTGTTTTATTATTANTGTTGTCTGGTTTGGTGGAAACTGGAGTANGGGCGGGGTGGACTATTTATCCCCCGTTATCTTCTANAGTANGACATANCGGAGTGTCAATAGATTTTGCTATTTTTTCTCTTCATTTAGCAGGGGCTTCTTCTATTATAGGAGCTATTAACTTTATTTCTACTGTTATAAATATACGTTTAATAGGGGTGGGTATAGATAAAATTAGCTTATTTGTATGATCAGTGGTGATTACGGCGGTTTTANTANTACTTTCTTTACCCGTATTAGCTGGAGCAATNACTATACTTTTGACTGA,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/SnapGene-Sequence-for-8-CO1-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_5002-scaled.jpeg,",47.5071822702,8.3789326508,08/19/2024,"
Caught on the leaf of a raspberry bush, frozen to death and stored in alcohol until DNA-extraction.<\/p>\n",Coreus marginatus,Instagene Matrix,Abdomen,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_6217.jpeg,","
ArthropodPCR-Gel: During this PCR round arthropodic DNA was targeted. The relevant sample is located in lane 8.<\/p>\n
As seen from the lack of band, something clearly went wrong. It is most likely that this happened in the sample preparation phase since even after repeating the PCR-process nothing showed up. This sample is, therefore, unusable.<\/p>\n",Unknown,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/14_Arthropod-CO1F-1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/original_55408965-fa7c-4762-b768-b998e170beae_PXL_20241007_150321119.jpg,",40.8758910000,-81.4023356000,10/06/2024,"
Centipede was found under rock and was very active.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/5-scaled.jpeg,",38.9345408991,-77.0716352597,09/04/2024,"
Under a couple of leaves of a bush, on top of a wooden plank, summer, 75\u00b0, terrestrial habitat<\/p>\n",Armadillidium,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/9_20_Wobach_gel_2-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_0214.png,","",No,GCTTGGGCTGCAATAGTGGGAACAGCAATAAGAGTANATTCGAATTGAGTTAGGTCAAACAGGTAGATTTATGGGTGATGATCAATTATATAATGTAATTGTAACGGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATACCAATTTTAATTGGAGGGTTTGGAAATTGATTAGTTCCATTAATATTAGGGGCACCTGACATAGCATTTCCTCGAATAAATAATTTGAGATTTTGATTATTACCCCCTTCTTTATTATTATTATTTATTTCGTCTATAGTAGAAATAGGAGTAGGAGCTGGGTGAACTGTATATCCACCTCTATCATCTTTAGATGGTCACGGTGGTAGATCAGTAGATTTTGCAATTTTTTCTTTGCATTTAGCTGGGGCTTCTTCTATTATAGGTGCGGTAAATTTTATTTCTACTGTACTCAATATGCGATCTTATGGGATGAGAATAGAAAAAGTTCCTTTATTTGTATGATCAGTTCTAATTACTGCTGTGCTATTATTATTATCATTACCAGTATTAGCAGGTGCTATTACTATATTATTAACTGATCGAAATTTTAATACTTCATTTTTTGATCCTGCAGGAGGAGGTGATCCTATTTTGTTTCAGCATTTATTCTGATTTT,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/NIU-5.jpg,",41.940959,-88.774038,09/12/2024,"
scarab beetle<\/p>\n",Coleoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/NIU-4.jpg,",41.940959,-88.774038,07/30/2024,"
ant<\/p>\n",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/NIU-3-.jpg,",41.940959,-88.774038,07/30/2024,"",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/NIU-2.jpg,",41.940959,-88.774038,07/30/2024,"",Hymenoptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/NIU-1-.jpg,",41.9315677000,-88.7636467000,07/30/2024,"",Diptera,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/tmp_54386736-3872-45e0-ae1a-7803931ea696-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/tmp_8bfff488-b890-4de6-8a1d-2693cf760cfc-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/tmp_79941916-492b-4e79-ab49-f264562e340f-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/tmp_b5944399-e1e7-437d-9f57-a2b59571f72a-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot_20241114_090534_Chrome.jpg,",47.5184073993,8.5374305009,08/28/2024,"
Im Spinnennetz im Keller eines Geb\u00e4udes. Trockenluft.<\/p>\n",Steatoda triangulosa,Instagene Matrix,Whole arthropod,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolb-2024-saz18-4-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolb-2024-saz7-3-scaled.jpg,","
1. Vortex und Zentrifuge verwechselt
\n2. Zu kurz gevortext
\n3. Falsch zentrifugiert<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/29_Arthropod-CO1F-1-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_3610-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_3608-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_3607-scaled.jpeg,",47.5039822862,8.5490087610,08/25/2024,"
In gras next to stream.<\/p>\n",Episyrphus balteatus,Instagene Matrix,Rear half,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_2443.jpeg,","
Reasons why it didn\u2018t work during the first try (Picture 1):<\/p>\n
– Foam formation (broken machine)<\/p>\n
– to much\/few sample pipetted in the gel<\/p>\n
– Centrifuged for too little time<\/p>\n",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/26_Arthropod-CO1F_Kreis.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Snap_29-08-2024_025-1-scaled.jpeg,",47.5072133910,8.3789972963,08/18/2024,"
Caught alive in grass, frozen to death and stored in alcohol until DNA-extraction<\/p>\n",Formica,Instagene Matrix,Whole arthropod,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_6216.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_6219.jpeg,","
ArthropodPCR-Gel2: During this PCR round arthropodic DNA was targeted. The relevant sample is located in lane 7.<\/p>\n
WolbachiaPCR-Gel9: Here wolbachia was targeted. The relevant sample is located in lane 8.<\/p>\n
The \u00a0results show that the sample was infected with wolbachia<\/p>\n",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/15_Arthropod-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Image-9-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Fly_December_2007-4-1.jpg,",47.5183676082,8.5373151711,08/28/2024,"
Die Probe wurde auf einer wand im einem Keller einer Wohnung gefunden. Der Keller war ziemlich trocken.<\/p>\n",Cecidomyiidae,Instagene Matrix,body without wings,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Medien-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Medien.jpeg,","
\n
Vortex und Zentrifuge verwechselt<\/li>\n
Zu kurz gevortext<\/li>\n
Falsch zentrifugiert<\/li>\n
Diese Probe (hier also 02) ist #2<\/li>\n<\/ol>\n",No,Konsensus,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/30_Arthropod-CO1F-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/tmp_f11f9b9a-4dea-4d32-a936-3c0399577e63-1-scaled.jpeg,",47.5710109603,8.5312370144,08/28/2024,"
Das Insekt wirde auf einem Sportplatz einer Grundschule gefangen. Es war steinig\/sandig am Rande eines Betonfussbalfeldes.<\/p>\n",Formicidae,Instagene Matrix,Abdomen,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolb-2024-saz7-2-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolb-2024-saz18-3-scaled.jpg,","
1. Vortex und Zentrifuge verwechselt
\n2. Zu kurz gevortext
\n3. Falsch zentrifugiert<\/p>\n
Anschliessend korrigiert<\/p>\n",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/32_Arthropod-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Snap_29-08-2024_019-scaled.jpeg,",47.5072000917,8.3789765595,08/18/2024,"
Caught alive on apple tree, frozen to death and stored in ethanol until DNA-extraction<\/p>\n",Sarcophaga carnaria,Instagene Matrix,Abdomen,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_6215.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_6218.jpeg,","
ArthropodPCR-Gel2: During this PCR round arthropodic DNA was targeted. The relevant sample is located in lane 6.<\/p>\n
WolbachiaPCR-Gel9: Here wolbachia was targeted. The relevant sample is located in lane 7.<\/p>\n
The \u00a0results show that the sample was not infected with wolbachia<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/6896A751-2008-4D5B-83BA-FB6DA38A511F.jpeg,",47.5023968114,8.4046265546,08/25/2024,"
captured: in the garden on a pear in Oberweningen<\/p>\n
condition: dead when caught, then frozen and preserved in alcohol<\/p>\n",Vespidae,Instagene Matrix,,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/919C3332-604B-4FB5-9A2B-36E850D7630E-scaled.jpeg,","
In both PCR processes meaningful\/good results could be observed. We refer to the results in lane 8 (picture 1) and lane 3 (picture 2).<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/8_Arthropod-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/tmp_27a57179-9a11-465c-968b-1990bbce3626-3-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/Screenshot_20241114_091303_Chrome.jpg,",47.5722722053,8.5191249235,09/28/2024,"
Vor der Haust\u00fcr im Garten, auf einer gem\u00e4hten Wiese. Keine Blumen, B\u00fcsche oder B\u00e4ume, nur Rasen.<\/p>\n",Anthomyiidae,Instagene Matrix,Abdomen,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolb-2024-saz18-2-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolb-2024-saz7-1-scaled.jpg,","
1. Wir haben Vortex zun\u00e4chst mit einer Zentrifuge verwechselt, anschliessend haben wir versucht es auszugleichen.
\n2. Wir haben zu kurz gevortext.
\n3. Wir haben zu kurz zentrifugiert<\/p>\n",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/31_Arthropod-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_7364-scaled.jpeg,",47.4436581941,8.5292963375,08/25/2024,"
I found it on a gras field in a neighbourhood.<\/p>\n",Formica cinerea,Instagene Matrix,body without wings,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/IMG_2443-2.jpeg,","
Reasons why it didn\u2018t work during the first try (Picture 1):<\/p>\n
– Foam formation (broken machine)<\/p>\n
– to much\/few sample pipetted in the gel<\/p>\n
– Centrifuged for too little time<\/p>\n",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/25_Arthropod-CO1F_Kreis.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/shared-image-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/shared-image-2.jpg,",47.4443969346,8.5274747163,08/25/2024,"
On a little hill behind a tree in the high gras. I cought it with a big net.<\/p>\n",Notostira erratica,Instagene Matrix,body without wings,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolb-2024-saz19-Davidstern02.png,","
Runned smothly, first lane in the image\u00a0 mid faint<\/p>\n",No,TCNTTAANANGAATTATTCGTATTGAACTANGAATACCTGNNNNNNTTATTGGAGATGATCAAACATATAATGTTGTAGT AACANCTCACGCATTCATCATAATCTTTTTTATANTAANNNNNNTTANNNNTGGGNNNNNNNNNNNNNNNNNNNNNCCNT TAANAATTNNNNCNNNNNNNNNNNNNNNNCCNCNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNCCNTAT NATTAT,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/18_Arthropod-CO1F-Davidstern.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Snap_29-08-2024_022-scaled.jpeg,",47.5179306706,8.5394361343,08/19/2024,"
$\"\"$ This sample was found in the attic and was already dead. It was conserved in alcohol until DNA was extracted.<\/p>\n",Ichneumonidae,Instagene Matrix,Whole arthropod,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Screenshot_20241024_092435_Samsung-Notes.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Screenshot_20241024_092325_Samsung-Notes.jpg,","
ArthropodPCR-Gel2: During this PCR round arthropodic DNA was targeted. The relevant sample is located in lane 5.<\/p>\n
WolbachiaPCR-Gel9: Here wolbachia was targeted. The relevant sample is located in lane 6.<\/p>\n
The \u00a0results show that the sample was not infected with wolbachia<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/13_Arthropod-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/7C918D12-666D-48FC-B654-E39076A04247.jpeg,",47.5023453311,8.4046293655,08/25/2024,"
captured: on the wall of the garage at home in Oberweningen<\/p>\n
condition: caught alive, then frozen to death and preserved in alcohol<\/p>\n",Araneae,Instagene Matrix,Abdomen,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/61FF5869-1209-41B0-9CAA-3604BCFC2D32-scaled.jpeg,","
In the PCR process no results could be observed. There must have happened a mistake during the DNA extraction. We refer to lane 7 (picture 1).<\/p>\n",Unknown,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/6_Arthropod-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/A5AF5952-2E7A-4DAD-8741-1FF028A8616B.jpeg,",47.5023415766,8.4046442151,08/25/2024,"
Captured: In the garden at home in Oberweningen<\/p>\n
Condition: Alive when caught and then frozen to death and then preserved in alcohol<\/p>\n",Diptera,Instagene Matrix,Abdomen,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/7655815C-0AAC-4E75-8658-CC8AC7477216-scaled.jpeg,","
In both PCR processes meaningful\/good results could be observed. We refer to the results in lane 6 (picture 1) and lane 2 (picture 2).<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/2EE37D73-3547-49A3-BE79-CFDEF61DE18A.jpeg,",47.4787519174,8.4897317008,08/25/2024,"
Captured: At home in the house in Niederhasli
\nCondition: Alive when caught and then frozen to death and preserved in alcohol<\/p>\n",Blattella germanica,Instagene Matrix,Abdomen,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/F1726A31-A6BF-4692-B19D-3BBBE573858E-scaled.jpeg,","
In both PCR processes meaningful\/good results could be observed. We refer to the results in lane 5 (picture 1) and lane 1 (picture 2).<\/p>\n",No,,,, "",47.45229682664101,8.53093365277366,,"",Arthropoda,,,,,,"","
only one out of 4 succeeded
\n3 probably failed during extraction<\/p>\n",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/fire-ant-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/fire-ant-2-scaled.jpeg,",26.5317866,-80.0905465,10/02/2024,"
I found this ant on the sidewalk near an apartment complex. I collected it about 6 am.<\/p>\n",Solenopsis,DNeasy (Qiagen),Abdomen,MiniPCR,TAE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Screenshot-5.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_0076-scaled.jpeg,",26.6937948,-80.0430028,09/19/2024,"",Arthropoda,pcr,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_0097.jpeg,","
Gel electrophoresis only occurred for 15 minutes due to time constraints<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_20240925_131336920_HDR-scaled.jpg,",26.7051402,-80.0498511,09/28/2024,"",Formicidae,pcr,,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_20241002_155335623-scaled-e1729639410255.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Adobe-Scan-Oct-14-2024-images-0-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Adobe-Scan-Oct-14-2024-images-1-1-scaled.jpg,",26.7062975818,-80.0535520264,09/25/2024,"
This cricket was kept in captivity, primarily used to feel reptile pets.<\/p>\n",Acheta domesticus,tweezers,Gonads dissected,Standard electrophoresis system,TAE,Other,"","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Acheta-domestica.jpg,",26.7049367,-80.0531524,09/26/2024,"
Purchased from Petco via brand Timberline Fresh.<\/p>\n",Acheta domesticus,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolbachia-gel.jpeg,","
The PCR products were run along a ThermoFisher Scientific GeneRuler 100 bp Ladder on a 2% agarose gel, as shown in table 3 (ThermoFisher Scientific 2024). 1X TAE was used in the gel and for running buffer, with 20,000X SmartGlow, a BioRad electrophoresis chamber and FisherScientific power source. The gel was run at 100 volts for 35 minutes and visualized using a Spectroline Ultraviolet Transilluminator.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Snapchat-1608813633.jpg,",26.7042790000,-80.0525744000,09/18/2024,"
Ant; smaller than a grain of rice<\/p>\n",Arthropoda,pcr,Whole arthropod,Standard electrophoresis system,TAE,Other,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Snapchat-1432190198-1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/INS-6540.jpg,",47.4944219937,8.5365823198,08/19/2024,"",Arthropoda,Instagene Matrix,Abdomen,MiniOne,,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/new_samp.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/my_sampl.png,","
The results of the my sample and the new sample are the same, except for the fact that in the new PCR test there is one sample less. That is because there was not enough DNA material left after the first PCR test for the second one. Since there was not enough DNA extract, it was imposible to get a new sequence.<\/p>\n
It is worth to mention that that sample (3B) had the strongest results in the first PCR test and it is a pity that the amount of DNA extract was too little to be able to do a second test. 3B was tested positive for Arthropode and Wolbachia. 1B, 1V had absolutely no results and 2V was tested positive for Arhtropode only.<\/p>\n
In comparison, the first and second test had the same results which probably indicates that the filling in into the moulds in the gel had to be done the same, most likely correctly. What could have been done better is to increase the amount of DNA extract to have enough for the second test but since we did not know about that, I would not really count that as a “mistake”.<\/p>\n",Yes,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/46_Arthropod-CO1F-Flower.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_7613-scaled.jpeg,",,,08/21/2024,"
Found in the forest
\ncondition: dead<\/p>\n",Arthropoda,Instagene Matrix,Abdomen,MiniOne,,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolb-2024-saz15-scaled.jpeg,","
In both PCR processes strong results were observed.<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/56_Arthropod-CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_7603-scaled.jpeg,",47.5626514,8.4956909,08/21/2024,"
Captured: Glattfelden forest
\nCondition: Dead when found (did not freeze to death)<\/p>\n",Arthropoda,Instagene Matrix,Abdomen,MiniOne,,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolb-2024-stud-12-2-scaled.jpeg,","
In both PCR processes strong results were observed.<\/p>\n",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/11/55_Arthropod-CO1F-1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_7619-scaled.jpeg,",,,08/20/2024,"
Caught in my garden<\/p>\n",Arthropoda,Instagene Matrix,Abdomen,MiniOne,,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolb-2024-stud-12-2-scaled.jpeg,","
Something went wrong during the DNA extraction because there was no sign of a line.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_7622-scaled.jpeg,",,,08/20/2024,"
Caught in my garden<\/p>\n",Arthropoda,Instagene Matrix,Abdomen,MiniOne,,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolb-2024-stud-12-2-scaled.jpeg,","
Something went wrong during the DNA extraction because there was no sign of a line.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/06.jpg,",47.57192849890741,8.530579471295633,,"
in garden<\/p>\n",Araneae,Instagene Matrix,Abdomen,MiniOne,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolb-2024-saz15-2-scaled.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/05.jpg,",47.57198538596588,8.53061182241014,08/20/2024,"
in garden on leaf of a raspberry bush<\/p>\n",Nezara viridula,Instagene Matrix,Whole arthropod,MiniOne,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolb-2024-saz15-2-scaled.jpg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/02.jpg,",47.5719558037,8.5305737415,08/20/2024,"
flying around a light source on a balcony<\/p>\n",Plagiognathus longipennis,Instagene Matrix,,MiniOne,,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolb-2024-saz15-1-scaled.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/01.jpg,",47.5720026975,8.5306041824,08/20/2024,"
in garden in a yew hedge<\/p>\n",Araneae,Instagene Matrix,Whole arthropod,MiniOne,,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolb-2024-saz15-scaled.jpg,","",No,,,, "",47.5200509144,8.5280918091,08/26/2024,"",,Instagene Matrix,Other,MiniOne,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Screenshot-2024-10-22-212603.png,","
We only got one Wolbachia band. This could be due to the DNA extraction process. Especially where we tried to take out the ovaries of our arthropodes. This was very difficult and didn’t work out well so we used a big part of the abdomen instead which might’ve lead to problems during the PCR.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/f1050a57-7de7-4a00-96b2-4cac9e316f80.jpeg,",47.5168606761,8.5354628321,,"
This leafhopper was caught on the ground in a flower bed which is located in the garden.<\/p>\n",Arthropoda,Instagene Matrix,Partial abdomen,MiniOne,,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolb-2024-saz17-3-scaled.jpeg,","
This sample showed the DNA in the second PCR run. More precisely, in our first PCR no results were observable. Given that there was a result in the second time, there must have occured an error in the PCR process (e.g incorrect amount of a solution, wrong order of steps or wrong execution of single steps).<\/p>\n",Yes,,,, "",47.509856,8.5938067,08/21/2024,"",,Instagene Matrix,Partial abdomen,MiniOne,,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_1019-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_1018-1.jpeg,","
– we had some difficulties with the pipettes, so the measurements may not have been very exact, which is one reason why some of our samples didn\u2019t work as well
\n– also some of our samples were just way too little and nothing was left after the procedure<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/PHOTO-2024-10-22-15-59-26.jpeg,",47.516856751971,8.535449221244201,08/21/2024,"
This arthropod was found in a garden on a leaf of a raspberry bush in the late afternoon.<\/p>\n",Arthropoda,Instagene Matrix,Abdomen,MiniOne,,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolb-2024-saz17-1-scaled.jpeg,","
This sample showed the DNA in the second PCR run. More precisely, in our first PCR no results were observable. Given that there was a result in the second time, there must have occured an error in the PCR process (e.g incorrect amount of a solution, wrong order of steps or wrong execution of single steps).<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_4131.jpeg,",47.5762223000,8.5219809000,08/20/2024,"
I found it on a leaf of a hazelnut tree.<\/p>\n",Philaenus spumarius,Instagene Matrix,Abdomen,MiniOne,,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolb-2024-saz17-2-scaled.jpeg,","
This sample showed the DNA in the final PCR. However, in our fist PCR no results showed. The DNA was still on top and didn\u2018t come down, the marker worked though. This might be due to an error in the process. Since it worked the second time, there has to be an error in the PCR process and not in the DNA extraction. That might be a wrong amount of a solution or the wrong order of steps.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/IMG_4312.jpeg,",47.5762223000,8.5219809000,08/20/2024,"
The bug was found near a flowerbed on a hot stone with in it\u2018s colony.<\/p>\n",Pyrrhocoris,Instagene Matrix,Abdomen,MiniOne,,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/Wolb-2024-saz17-scaled.jpeg,","
This bug didn\u2018t show any results on both PCRs. This might be due to an error in the DNA extraction or the PCR process. There is a possibility that some chemicals or bad conditions lead to the DNA degrading.<\/p>\n",Unknown,,,, "",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG_9926-scaled.jpeg,",37.8037496898,-122.4214773131,,"
Was almost near a pile of feces<\/p>\n",,,Abdomen,MiniOne,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/10/DCACA61E-0792-4A0C-97D5-DD5E5DDF2CC6.jpeg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG_0098.png,",90.3311700000,34.2385800000,09/15/2024,"
While observing the mosquito, it seems to be moving around a lot. At one point, it was hanging on to the jar.<\/p>\n",Aedes albopictus,,,,,,"","",,,,, "",32.3984904,-90.15652089999999,09/10/2024,"
The moth was flying fast until it was caught in the grass, when it was in the cup, it was still. The weezle was crawling on the concrete in front of the Kroger gymnasium. The insect was moving slowly.<\/p>\n",Coleoptera,DNeasy (Qiagen),,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG_1270-1-scaled.jpeg,",90.16220,32.40344,09/10/2024,"
The field cricket pooped alot inside the container it was in. It was also doing a lot of jumping.<\/p>\n",Gryllus,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/cricket-dead-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/cricket-in-cup-scaled.jpg,",32.3672122,-90.2338473,09/10/2024,"
The fall field cricket was very active while jumping around in its habitat. It was a dark brownish color with antennas<\/p>\n",Gryllus pennsylvanicus,DNeasy (Qiagen),Abdomen,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/stinkbug-in-cup-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/stinkbug-dead-scaled.jpg,",32.4048193,-90.160845,09/13/2024,"
The stink bug was very active and feisty with a black outercovering and long legs.<\/p>\n",Proxys punctulatus,DNeasy (Qiagen),Abdomen,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG_7122-scaled.jpeg,",41.902170,-71456490,09/18/2024,"
Has wings<\/p>\n
small<\/p>\n
light organs underneath abdomen<\/p>\n",Coleoptera,,,,,,"","",,,,, "",,,09/18/2024,"
Small, Brown<\/p>\n",Arthropoda,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG_8951-1-scaled.jpeg,",32.40317975592171,-90.16194627512107,09/10/2024,"
While observing Indian meal moths behind the AA Branch at Tougaloo College, I noticed they are strongly attracted to food sources, especially grains and stored products like flour and cereals. The adult moths are small, with brownish wings and lighter tips that help them blend into their surroundings, and they flutter around erratically when disturbed. Most of their activity happens in the late afternoon and evening, likely as they search for mates or food. I also spotted their larvae, which are small and whitish, feeding on the same food sources and leaving behind webbing and frass, a clear sign of infestation. Overall, these moths display typical foraging and reproductive behaviors in environments where food is abundant.<\/p>\n",Arthropoda,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG_8950-scaled.jpeg,",32.4032567518,-90.1619500701,09/10/2024,"
Observing house crickets behind the AA Branch at Tougaloo College reveals some interesting behaviors. They are most active during the warmer parts of the day, particularly in the early evening, and their rhythmic chirping suggests mating calls. These crickets prefer areas with dense vegetation, like tall grass and shrubs, providing shelter from predators. They often hide under leaves or in crevices and come out to feed on plant material. Overall, their behaviors demonstrate effective strategies for mating, feeding, and evading threats in their natural habitat.<\/p>\n",Arthropoda,,,,,,"","",Unknown,,,, "",90.1620788,32.4032659,09/10/2024,"
This arthropod was very active. When captured the arthropods movement became more rapid.<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Abdomen,,,,"","",,,,, "",,,,"",,,,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG_5691-rotated.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG_5690-rotated.jpg,",32.4032769000,-90.1621053000,09/13/2024,"
At first glance it was have thought to have been a ladybug sitting on a leaf and flying around.<\/p>\n",Neolema sexpunctata,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG_5663-rotated.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG_5667-rotated.jpg,",32.4032699000,-90.1620755000,09/10/2024,"
Before being caught the cricket was in its own environment hopping around in the grass.<\/p>\n",Gryllus,DNeasy (Qiagen) blood and tissue kit,Abdomen,,,,"","",,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/unnamed-3.jpg,",37.7597412,-122.4011391,09/03/2024,"
Raised in a mosquito breeder, female mosquito with no furry antennas<\/p>\n",Culex pipiens,BABEC,Whole arthropod,MiniOne,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/unnamed-4.jpg,","
BABEC’s protocol<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/unnamed-2-scaled.jpg,",37.8043856,-122.4210043,09/03/2024,"",Hymenoptera,BABEC,Whole arthropod,MiniOne,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG_1724-1-scaled.jpeg,","
BABEC’s protocol<\/p>\n",Yes,,,, "",37.8043856,-122.4210043,09/03/2024,"",Aedes,BABEC,Whole arthropod,MiniOne,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Screenshot-2024-09-20-3.19.52-PM-1.png,","
Taq polymerase used<\/p>\n
Annealing temperatures for each PCR reaction was 55 degrees celsius.<\/p>\n
6th well starting from left to right a<\/p>\n",Yes,,,, "",37.8043856000,-122.4210043000,09/03/2024,"
Black, 6 legs, 2 wings, 2 eyes, 2 antennaes<\/p>\n",Insecta,BABEC,Abdomen,MiniPCR,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Screenshot-2024-09-20-3.29.01-PM.png,","
Taq polymerase<\/p>\n
Annealing temperatures for each PCR reaction.<\/p>\n",No,,,, "",37.8043856000,-122.4210043000,09/03/2024,"
\n
Beige colored<\/li>\n
Black and white pattern on abdomen<\/li>\n
8 legs<\/li>\n
8 eyes<\/li>\n
Hairy legs and abdomen<\/li>\n
2 mouth parts<\/li>\n<\/ul>\n",Arachnida,BABEC,Abdomen,MiniOne,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Screenshot-2024-09-20-3.19.52-PM.png,","
Taq polymerase was used.<\/p>\n
Annealing temperature for each PCR reaction was 55 degrees celsius.<\/p>\n
5th well starting from the left to right<\/p>\n",Yes,,,, "",37.8040555644,-122.4208217323,,"
It was about the size of a grain of rice.
\nIt was a dark brown colour.
\nIt was found on a wall with shade and wet.<\/p>\n",Coleoptera,BABEC,Whole arthropod,MiniOne,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG_0290-scaled.jpeg,","
– Taq polymerase used
\n– Annealing temperature 55 C<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/6F9B172C-63EA-46E9-9E47-2AFCF35FC089_1_201_a.jpeg,",37.804278237689324,-122.42088850969355,09/03/2024,"",Arthropoda,BABEC,Abdomen,MiniOne,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/025D2B12-8295-4123-878D-0FAC88E2A600_1_201_a.jpeg,","
BABEC<\/p>\n",Yes,,,, "",37.8037628000,-122.4246109000,09/03/2024,"
The mosquito was tiny and dissected.<\/p>\n",Arthropoda,BABEC,Abdomen,MiniPCR,TAE,GelGreen,"","
Used BABEC<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG_1310-scaled.jpeg,",37.8043011946,-122.4210537227,09/03/2024,"
Had an egg sack<\/p>\n",Theridiidae,BABEC,Abdomen,,,,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG_1379-scaled.jpeg,","
Taq polymerase used<\/p>\n
Annealing temperature 55C<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG_8754.jpeg,",37.7597412,-122.4011391,09/03/2024,"
Male mosquito, furry antennas<\/p>\n",Culex pipiens,BABEC,Whole arthropod,MiniOne,TAE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG_8757.jpeg,","
BABEC<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG_1568.jpeg,",,,,"
Mosquito<\/p><\/blockquote>\n",,pcr,Whole arthropod,,TAE,GelGreen,"","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG_3054.jpeg,",,,09/03/2024,"",Hymenoptera,BABEC,Whole arthropod,MiniOne,TAE,GelGreen,"","",No,,,, "",,,,"
<\/p><\/blockquote>\n",,BABEC,Rear half,MiniOne,TAE,GelGreen,"","
<\/p><\/blockquote>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Image-2.jpeg,",47.3928307894,9.6298977179,08/28/2024,"
auf gr\u00fcnen Bl\u00e4ttern von Laubbaum, ca.1m H\u00f6he<\/p>\n",Palomena prasina,Instagene Matrix,,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Image-3.jpeg,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Image-4.jpeg,",47.3926300666,9.6300806235,08/28/2024,"
auf gr\u00fcnen Bl\u00e4ttern von Laubbaum, ca. 1m H\u00f6he<\/p>\n",Anoecia corni,Instagene Matrix,Partial abdomen,Standard electrophoresis system,TAE,,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Image-5-1.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Wolbachia_YG2.jpg,",47.4032432856,9.6178819587,08/28/2024,"
On deciduous tree, approx. 1m high, mixed forest<\/p>\n",Lasius niger,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Gel_YG2.png,","",Unknown,CATTCGACTAGAATTAGGTTCTTCTAATTCATTAATTAATAATGATCAAATTTATAATTCTATAGTTACAAGACATGCATTCGTTATAATTTTCTTTATAGTTATGCCTTTCATAATTGGAGGATTTGGAAATTTTCTCGTACCTTTAATATTAGGATCACCTGATATAGCATACCCCCGTATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATTTCTCTCCTTCTTTTAAGAAATTTTATTAATGACGGAGTAGGAACAGGA,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/YG2_CO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Wolbachia_YG1-1.jpg,",47.4032575087,9.6178690843,08/28/2024,"
Semi-illuminated forest, near water, mixed forest<\/p>\n",Aedes vexans,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Gel_YG1.png,","",Unknown,TAGTAGGAACATCTTTAAGTATATTAATTCGTGCTGAATTAAGACACCCAGGGATATTTATTGGAAATGATCAAATTTATAACGTAATTGTTACAGCTCATGCATTTATTATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGATTCGGAAATTGATTAGTTCCTTTAATATTAGGAGCTCCTGATATAGCTTTTCCTCGAATAAATAATATAAGTTTTTGAATATTACCTCCTTCATTGACTCTACTACTTTCTAGTTCAATAGTAGAAAATGGAGCAGGGACAGGATGAACAGTTATCCTCCTCTTTCATCAGGAACAGCTCACGCTG,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/YG1_CO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/LH2_1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/LH2_2.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/LH2_3.jpeg,",47.4028976489,9.6198487056,08/28/2024,"
in loose soil covered with moos on forest floor next to a path in a mixed forest, under moist disintegrating dead wood<\/p>\n",Cylindroiulus britannicus,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Image-1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/LH1_1-1.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/LH1_2-1.jpeg,",47.4028655614,9.6198427480,08/28/2024,"
Next to a path in a mixed forest, half-shade<\/p>\n",Aedes cinereus,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Image-1.jpg,","",Yes,ATAGTAGGAACATCTTTAAGTATATTAATTCGTGCAGAATTAAGTCATCCAAGTATATTTATTGGAAATGACCAAATTTATAATGTAATTGTAACAGCTCATGCATTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTTGGTAATTGACTTGTTCCTTTAATATTAGGAGCCCCTGATATAGCCTTTCCTCGAATAAATAATATAAGTTTTTGAATATTACCTCCCTCATTAACCCTGTTATTATCAAGCTCAATAGTAGAAAATGGAGCTGGTACAGGGTGAACTGTTTACCCTCCTTTATCTTCTGGAACTGCTCATGCAGGAGGTTCTGTTGACTTAGCAATTTTCTCTTTACATTTAGCCGGGATTTCTTCGATTTTAGGGGCCGTAAATTTTATTACAACTGTTATTAATATGCGATCAGCAGGAATTACACTTGATCGACTTCCTTTATTTGTTTGATCAGTTGTTATTACTGCTATTTTATTACTTCTTTCTTTACCTGTTTTAGCTGGGGCTATTACTATATTATTAACTGATCGAAATTTAAATACATCA,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/LH1_CO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/WhatsApp-Bild-2024-08-29-um-16.45.54_12503376888-scaled.jpg,",47.4035308627,9.6179498800,08/28/2024,"
Habitat: Forest (warm, bright location)<\/p>\n",Culicidae,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Gel-Image-Rea_Muriel_Annika-2.png,","",Yes,RTTATACCTATTATAATTGGAGGGTTTGGAAATTGACTAGTTCCTTTAATATTAGGAGCTCCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTTTGAATATTACCCCCTTCCTTAACCTTACTACTTTCAAGTAGAATGGTAGAAAATGGGTCTGGAACTGGATGAACTGTTTATCCCCCCCTTTCTTCAGGAACTGCTCATGCAGGAGCTTCAGTAGATTTAACTATTTTTTCTTTACATTTAGCCGGGATTTCCTCAATTTTAGGAGCAGTAAATTTTATTACGACTGTAATTAATATACGATCTGCTGGAATTACTTTAGACCGACTACCTTTATTTGTATGATCTGTAGTAATTACAGCTGTATTATTACTTTTATCTCTCCCTGTATTAGCTGGTGCAATTACTATATTATTAACTGACCGAAACTTAAATACTTCTTTCTTTGACCCAATTGGAGGAGGAGACCCTATTCTTTACCAACATTTATTTTGATTTTTTGGTCACCTKA,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/MS2_CO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Image-1.jpeg,",47.4031381003,9.6178624591,08/28/2024,"
Semi-illuminated forest, near water, mixed forest<\/p>\n",Trichoceridae,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Picture1.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/cdf185c7-173d-4f7b-bca5-5daaf193ff36.jpg,",47.4032173388,9.6188448766,,"
grass, forest edge<\/p>\n",Paederus riparius,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Screenshot-2024-09-11-112614.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Image.jpeg,",47.4031970118,9.6179123562,08/28/2024,"
Forest ground, near water, mixed forest<\/p>\n",Myrmica rubra,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Picture2.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/506214e5-d0e5-429f-be69-4420549beb1f.jpg,",47.4031025271,9.6186366978,08/28/2024,"
Forest path, earth, forest edge<\/p>\n",Formica rufa,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Screenshot-2024-09-11-111427.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Heugumper-1.jpg,",47.4031544000,9.6178862000,08/28/2024,"
Graswiese (sonniger Standort)<\/div>\n",Pseudochorthippus parallelus,Instagene Matrix,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/sequence-2024-09-16-08_19_08-2.jpg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG-20240901-WA0000.jpg,",47.4033900120,9.6178898920,08/28/2024,"
Habitat: Approx. 1.50m above the forest floor in an area where the rays of the sun reach well.<\/p>\n",Anoecia corni,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Gel-Image-Rea_Muriel_Annika-1.png,","",No,CTTCACTTAGAATATTAATTCGACTTGAACTTAGACAAATTAATTCTATTATTAATAATAACCAATTATATAATGTAATTATTACAATTCATGCWTTTATCATAATTTTTTTTATAACTATACCAATTGTTATTGGAGGTTTTGGAAATTGATTAATTCCTTTAATAATAGGATGTCCCCGATATATCWTTTCYACGACSTYAATAATATTAGATTTTGATTACTACCACCCTCATTAATAATAATAATCTGCAGATTTATTATTAATAATGGAACAGGAACAGGTTGAACTATTTACCCCCCTCTTTCTAATAATATTGCCCATAATAATATTTCAGTAGATTTAACTATTTTTTTCATTACATTTAGCAGGAATTTC,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/AM2_CO1-1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Ameise-Ass-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Ameise-Chopf-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Ameise-3.jpg,",47.4031545924,9.6179808081,08/28/2024,"
Habitat:<\/p>\n
Waldweg (erdiger Boden)<\/div>\n",Myrmica scabrinodis,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/sequence-2024-09-16-08_19_08.jpg,","",No,TTAGAATTAGGATCTTGTAACCCATTAATTAATAATGATCAAATTTATAATTCTCTTGTAACTAGCCATGCCTTTATTATAATTTTTTTTATAGTTATACCATTTATAATTGGGGGCTTTGGTAATTTTTTAATCCCTTTAATATTAGGGTCACCTGATATAGCATACCCACGAATAAATAACATAAGATTTTGATTACTTCCCCCTTCAATTATACTATTAATATTAAGAAATTTTTTAAGGAATGGAGTA,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/LE1_CO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/LG1-2.jpeg,",47.4024458596,9.6185549726,05/03/2030,"",,pcr,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Gel-2.jpg,","",Unknown,AGTAGGAACTTCTTTAAGAATTCTTATTCGAGCTGAATTAGGACACCCAGGAGCATTAATTGGAGATGATCAAATTTATAATGTGATCGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATACTAGGAGCTCCTGATATAGCTTTTCCCCGAATGAATAATATAAGTTTTTGACTTTTACCTCCTGCATTAACTTTATTATTAGTTAGTAGTATAGTAGAAAACGGAGCTGGAACAGGATGAACTG,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/LG1_CO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/LG2-scaled.jpeg,",47.4027893223,9.6181907723,02/11/2024,"",,pcr,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Gel-1.jpg,","",Yes,TAGGATCTTGTAACCCATTAATTAATAATGATCAAATTTATAATTCTCTTGTAACTAGCCATGCCTTTATTATAATTTTTTTTATA,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/LG2_CO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/gemeine-Rasenameise-scaled.jpg,",47.4023778996,9.6185811215,08/28/2024,"",,Instagene Matrix,,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Nachweis-von-Wolbachia_ST_EH_LG.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/WhatsApp-Bild-2024-08-29-um-16.45.53_a7901e2f-scaled.jpg,",47.4033349692,9.6178684315,08/28/2024,"
Habitat: Forest path (between gravel, leaves and soil)<\/p>\n",Hymenoptera,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Gel-Image-Rea_Muriel_Annika-2.png,","",No,CCATTAATTAATAATGATCAAATTTATAATTCTCTTGTAACTAGCCATGCCTTTATTATAATTTTTTTTATAGTTATACCATTTATAATTGGGGGCTTTGGTAATTTTTTAATCCCTTTAATATTAGGGTCACCTGATATAGCATACCCACGAATAAATAACATAAGATTTTGATTACTTCCCCCTTCAATTATACTATTAATATTAAGAAATTTTTTAAGGAATGGAGTAGGTACAGGATGAACCATTTACCCCCCTCTAGCTTCTAATATTTTCCATAGAGGACCTTCAATTGATATATCAATCTTTTCTCTTCATATCGCTGGAATATCATCTATTTTAGGTGCTATTAATTTTATCTCAACAATTATTAATATACATCATAAATCAGTATCAATAGACAAAATCCCCTTAATAGTATGATCAATTTTAATCACAGCTATTCTTCTCTTACTTTCCTTACCAGTCTTAGCAGGAGCAATTACTATATTATTAACAGATCGTAATTTAAATACTTCTTTTTTCGACCCATCTGGAGGGGGAGATCCAATTTTATACCAACATCTATTTTGATTTTTTGGTCACCTKKAAAGTT,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/MS1_CO1-1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/37e03bfb-e381-4f26-ae9e-de9f883b8dfd-1_all_18786-2.jpg,",47.4067767000,9.6059622000,08/28/2024,"
Approx. 1.50 metres above the forest floor in an area where the sun’s rays reach well.<\/p>\n",Hemiptera,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Gel-Image-Rea_Muriel_Annika-4.png,","",Yes,TTAGGATCTTGTAACCCATTAATTAATAATGATCAAATTTATAATTCTCTTGTAACTAGCCATGCCTTTATTATAATTTTTTTTATAGTTATACCATTTATAATTGGGGGCTTTGGTAATTTTTTAATCCCTTTAATATTAGGGTCACCTGATATAGCATACCCACGAATAAATAACATAAGATTTTGATTACTTCCCCCTTCAATTATACTATTAATATTAAGAAATTTTTTAAGGAATGGAGTAGGTACAGGATGAACCATTTACCCCCCTCTAGCTTCTAATATTTTCCATAGAGG,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/RS2_CO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG20240828145300-1.jpg,",47.4067767000,9.6059622000,08/28/2024,"
Forest path soil, near mixed forest foliage<\/p>\n",Hymenoptera,DNeasy (Qiagen),Abdomen,MiniPCR,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Gel-Image-Rea_Muriel_Annika-4.png,","
The gel image shows that no Wolbachia is present.<\/p>\n",No,GGAATCTCTTCTATTTTAGGAGCTATCAATTTTATTTGTACTATCCTAAATATAATACCTCATAATATAAAAATTAATCAAATCCCCCTATTCCCATGATCAATCTTAATTACAGCAACTTTATTAATTTTATCATTACCAGYTTTAGCTGGTGCAATTACTATATTATTAACTGATCGAAACCTAAATACATCTTTCTTTGACCCATCAGGAGGAGGAGATCCAATTTTATATCAACATCTATTCTGATTTTTTGG,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/RS1_CO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG-20240910-WA0000.jpg,",47.4033707616,9.6178547273,08/28/2024,"
Habitat: Path of a forest floor, near mixed forest foliage.<\/p>\n",Paederus littoralis,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Gel-Image-Rea_Muriel_Annika.png,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG-20240909-WA0006.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/IMG-20240909-WA0004.jpg,",,,09/02/2024,"",Vespula germanica,Instagene Matrix,Partial abdomen,Standard electrophoresis system,TAE,rotisafe gel stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Untitled-presentation.png,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/9_A-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/PXL_20240909_063647742-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/PXL_20240909_063809841-1-scaled.jpg,",,,09/08/2024,"",Vespa crabro,Instagene Matrix,Partial abdomen,Standard electrophoresis system,TAE,rotisafe gel stain,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/Untitled-presentation-1.png,","",No,,https://wolbachiaprojectdb.org/wp-content/uploads/2024/12/10_A-CO1F.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Image-8.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Image-1-1.jpeg,",47.4031337876,9.6191896862,08/28/2024,"
At the edge of a damp forest path of a mixed forest, that was partially covered by moss.<\/p>\n",Myrmica rubra,Instagene Matrix,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Image-2-1.jpeg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Fliege-2.jpg,",47.4031544000,9.6178862000,08/28/2024,"
Wald, warm.<\/p>\n",Musca domestica,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/sequence-2024-09-16-08_19_08-1.jpg,","",Yes,WATAGTAGGAACTTCTTTAAGAATTCTTATTCGGGCTGAATTAGGACACCCAGGAGCATTAATTGGAGATGACCAAATTTATAATGTAATTGTTACAGCCCATGCTTTCATTATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGAAATTGATTAGTCCCCCTAATATTAGGAGCCCCTGATATAGCCTTTCCCCGAATAAATAATATAAGTTTTTGACTTTTACCTCCTGCATTAACTTTATTATTAGTAAGTAGTATAGTAGAAAACGGAGCTGGAACAGGATGAACTGTTTACCCCCCTCTATCTTCAAATATTGCCCATGGTGGAGCTTCTGTTGATTTAGCAATTTTCTCTCTTCACTTAGCAGGAATTTCTTCTATTCTAGGGGCTGTAAATTTTATTACAACTGTGATTAATATACGTTCAACTGGAATTACATTTGATCGAATACCTTTATTTGTTTGATCAGTTGTTATTACAGCTTTATTACTTCTTTTATCTTTACCAGTTTTAGCAGGAGCAATTACTATATTATTAACAGATCGAAATTTAAACAYTTCATTTTTTGATCCAGCAGGAGGTGGAGATCCTATTTTATATCAACACTTATTTTGATTTTTTGGTCACT,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/AB2_CO1-2.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Ameise-4.jpg,",47.4031544000,9.6178862000,08/28/2024,"
earthy forest path<\/p>\n",Myrmica scabrinodis,Instagene Matrix,,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/sequence-2024-09-16-08_19_08-1.jpg,","",Yes,CTTCAATAAGAATAATTATTCGTTTAGAATTAGGATCTTGTAACCCATTAATTAATAATGATCAAATTTATAATTCTCTTGTAACTAGCCATGCCTTTATTATAATTTTTTTTATAGTTATACCATTTATAATTGGGGGGCTTTGGTAATTTTTTAA,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/AB1_CO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Eliana_Ameise_rot.jpg,",47.4032516595,9.6209280565,08/28/2024,"",Myrmica rubra,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Gelbild_beschriftet.jpg,","",No,TTAGAATTAGGATCTTGTAACCCATTAATTAATAATGATCAAATTTATAATTCTCTTGTAACTAGCCATGCCTTTATTATAATTTTTTTTATAGTTATACCATTTATAATTGGGGGCTTTGGTAATTTTTTAATTCCTTTAATATTAGGATCACCTGATATAGCATACCCACGAATAAATAACATAAGATTTTGATTACTTCCCCCTTCAATTATACTATTAATATTAAGAAATTTTTTAAGGAATGGTGTAGGTACAGGATGAACCATTTACCCCCCTCTAGCTTCTAATATTTTCCATAGAGGACCTTCAATTG,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/EH2_CO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Tabita_4.1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Tabita_4.2-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Tabita_4.3-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Tabita_4.4-scaled.jpg,",47.4033324157,9.6208867715,08/28/2024,"",Trachelipus,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/Gelbild_beschriftet.jpg,","",No,TACAGCAATTCTCTTATTACTTTCACTACCGGTTTAGCAGGGGCCATTACTATRTTATTAACAGACCGTAATTTGAACACCTCTTTTTTCGACCCTAGGGAG,https://wolbachiaprojectdb.org/wp-content/uploads/2024/09/TS1_CO1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/07/Sample-12-scaled.jpg,",33.6135351792,-86.6028674518,09/04/2023,"",Brachycybe rosea,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,GelRed,"","",No,NNNNTNTNNTTTTTGGTTCNTGAGCTGCAATGAGTGGAACGGCCTTAAGAATGTTAATTCGAATAGAGTTGGNTAANNCCTGGTAGTCTTATTGGGGATGATCAGATTTATAATGTTGTTGTGACGGCACATGCATTTGTAATAATTTTTTTTATGGTTATACCAATTATAATTGGTGGTTTTGGTAATTGGTTAGTTCCTCTAATATTAGGTGCTCCTGATATAGCATTTCCTCGAATAAATAATATGAGATTTTGATTATTGCCCCCAGCATTTTTTATATTGTTGTCATCATCATTAATAGAAAAAGGTGTAGGAACAGGTTGAACTGTTTATCCTCCTTTAGCTAGAAATGTTGCTCATAATGGAATGGCAGTTGATATAGCAATTTTTTCTTTACATTTAGCTGGAATTTCTTCAATTTTAGGAGCTATTAATTTTATTACTACTATTATTAATATACGGACAAGAGGAATATTATTTGAACGAATATCACTTTTTTTATGATCAGTAAAATTAACAGCTATTTTGTTGTTATTATCTTTGCCAGTATTGGCTGGAGCGATTACAATATTATTAACTGATCGAAATTTTAATACGTCTTTTTTTGATCCAGCAGGAGGGGGAGACCCTATTTTATATCAACATTTATTTTGATTTTTTGGTCACCCTGAAAGTTTAAAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/07/Sample-9-scaled.jpg,",46.9578113724731,-95.11047393583983,07/01/2022,"
Female<\/p>\n",Dermacentor variabilis,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,GelRed,"","",No,ACAACCACCCTCGGAGTTGAGCAGGANCACAGGATAAGAATAAGATTTTAATTCGAATAGAATTAAGCCAACCAGGAACTTTTAGTTGGAAATGATCAAATTTATAACGTAATTGTAACTGCTCATGCATTTATTATAATTTTTTTCATAGTTATGCCTATCATAATTGGTGGGTTTGGAAACTGACTTGTACCAATTATATTAAGGAGGCCCCAGATATAGCATTTCCACGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCATTATTTTTACTTATAAATTCTTCATTAATTGAATCAGGAGCAGGAACAGGTTGAACTGTTTATCCCCCTTTATCATCAAATTTGTCTCATTATGGACCTTCAGTTGATTTAGCTATTTTTAGCCTTCATCTAGCAGGTGCTTCTACTATTTAAGGAGCGATTAATTTTATTACCACAATTTTTAATACACGNCCAATTGGTTAAACTTTTGAACGAATACTTTTATTTGTTTGATCAGTTTTAATTACAGCAATTTTACTTCTCCTTCTCTCCTGTTTTAGCAGGAGCAATAACAAATTATTAAACAGACCGAAATTTANNACCTCCTTTTTGACCNTCCAGGTGGTGGAGACCCCTTTTGTTGCACCTCCACNTTAGATTTTTGTCACCNNNNNTNAAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/07/Sample-8-scaled.jpg,",33.61357091868815,-86.60286745180662,09/10/2024,"",Blattodea,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,GelRed,"","",Yes,GTAAACCNAANTTGGAACTGATCTGGATAGTGGGACTTCATTAAGAATATTAATTCGAGCCGAATTAAATCAACCAGGATCACTAATTGGAGATGACCAAATTTATAATGTTATTGTTACAGCTCACGCTTTTATTATAATTTTCTTTATAGTTATACCTATTTTAATTGGAGGATTTGGAAATTGATTAGTACCTTTAATATTAGGAGCACCTGATATAGCTTTCCCACGAATAAATAATATAAGATTTTGATTATTACCACCATCACTTTCATTATTATTAACAAGTAGTATGGTTGAAATAGGAGCTGGCACTGGATGAACCGTTTACCCCCCTCTAGCTAGAGGCATTGCCCATGAAGGGGCATCCGTAGACCTAGCAATCTTTTCATTACATTTAGCAGGAATTTCATCAATTTTAGGTGCAGTAAATTTTATTTCTACTACAATTAAAATAAAACCAAACTATATAAAACCTGATCAAATTCCTTTATTTGTATAAGCAGTNTCAATTACTGCCTTATTATNATAATAATCATTGCCTGTTTTAGCAGGTGCTATTACAATTTTTTTGACAGATCGAAACTTATATACTTCTTTTTTTGATCCTGCGGGTGGGGGTGATCCATTTTTATATCAACATNNTTCTGATTTTTGACAACACCAAAAAAAAAAAAAAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/07/Sample-6-scaled.jpg,",46.95874868577193,-95.10957247896539,07/03/2023,"
Female<\/p>\n",Dermacentor variabilis,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/07/CO1-PCR1-1-rotated.jpg,","",No,GCTTTTCTTATTTTCGGCAGTTGAGCAGGAGTCACAGGAATAAGAATAAGAATTTTAATTCGAATAGAATTAAGCCAACCAGGAACTTTAATTGGAAATGATCAAATTTATAACGTAATTGTAACTGCTCATGCATTTATTATAATTTTTTTCATAGTTATGCCTATCATAATTGGTGGGTTTGGAAACTGACTTGTACCAATTATATTAGGAGCCCCAGATATAGCATTTCCACGAATAAATAATATAAGATTTTGACTTTTACCTCCTTCATTATTTTTACTTATAAATTCTTCATTAATTGAATCAGGAGCAGGAACAGGTTGAACTGTTTATCCCCCTTTATCATCAAATTTGTCTCACTATGGACCTTCAGTTGATTTAGCTATTTTTAGCCTTCATCTAGCAGGTGCATCCTCTATTTTAGGAGCAATTAATTTTATTACTACAATTATTAATATACGATCAATTGGTTTAACTCTTGAACGAATACCTTTATTTGTTTGATCAGTTTTAATTACAGCAATTTTACTTCTTCTTTCTCTCCCTGTTTTAGCAGGAGCCATTACAATATTATTAACAGATCGAAACTTTAATACCTCCTTTTTTGATCCTTCAGGTGGTGGAGATCCCATTTTGTATCAGACCATTCTATTTTGATTTTTGGCACTCCA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/07/Sample-5-scaled.jpg,",46.9589830115,-95.1108172586,07/01/2022,"
Male<\/p>\n",Dermacentor variabilis,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,GelRed,"","",No,GGCTTTCTTATTTTCGGCAGTTGAGCAGGAGTCACAGGAATAAGAATAAGAATTTTAATTCGAATAGAATTAAGCCAACCAGGAACTTTAATTGGAAATGATCAAATTTATAACGTAATTGTAACTGCTCATGCATTTATTATAATTTTTTTCATAGTTATGCCTATCATAATTGGTGGGTTTGGAAACTGACTTGTACCAATTATATTAGGAGCCCCAGATATAGCATTTCCACGAATAAATAATATAAGATTTTGACTTTTACCTCCTTCATTATTTTTACTTATAAATTCTTCATTAATTGAATCAGGAGCAGGAACAGGTTGAACTGTTTATCCCCCTTTATCATCAAATTTGTCTCACTATGGACCTTCAGTTGATTTAGCTATTTTTAGCCTTCATCTAGCAGGTGCATCCTCTATTTTAGGAGCAATTAATTTTATTACCACAATTATTAATATACGATCAATTGGTTTAACTCTTGAACGAATGCCTTTATTTGTTTGATCAGTTTTAATTACAGCAATTTTACTTCTTCTTTCTCTCCCTGTTTTAGCAGGAGCCATTACAATATTATTAACAGATCGAAACTTTAATACCTCCTTTTTTGATCCTTCAGGTGGTGGAGATCCCATTTTGTATCAACATCTATTTTGATTTTTTGTCACTACAAAAAAATTAAAAAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/07/centipede.jpg,",46.4679893015,-80.9723997035,10/12/2023,"
This centipede was collected on the forest floor under natural cover on the Laurentian University campus.<\/p>\n",Chilopoda,DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL,Partial abdomen,Standard electrophoresis system,TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/07/Results-from-the-Arthropod-CO1-PCR-reaction-for-centipede-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/07/Results-from-the-Wolbachia-16S-rRNA-PCR-reaction-for-centipde-1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/07/Springtail.jpg,",46.4680528659,-80.9724802854,10/12/2023,"
The springtail was sampled in the fall from a forest found on campus. It was found under natural cover on the forest floor.<\/p>\n",,DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL,Whole arthropod,Standard electrophoresis system,TAE,GelRed,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Results-from-the-Arthropod-CO1-PCR-reaction.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Results-from-the-Wolbachia-16S-rRNA-PCR-reaction-1.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_4887WOLBACH1.png,",40.6209250000,-74.5702138900,05/28/2024,"
Ontholestes SP<\/em>\u00a0found under dead leaves, motionless until provoked, attempted to burrow underground. Three-segmented beetle, found with one pincer missing, shiny black hard abdomen section, six legs. Yellow and brown striped patterns across abdomen and lower areas, abdomen about 3 centimeters, full body about 6 centimeters in length, pointed middle segment and “tail” like 2mm appendages at end of abdomen.<\/p>\n",Ontholestes,DNeasy (Qiagen) blood and tissue kit,Partial abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Screen-Shot-2024-06-04-at-9.59.28-AM.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Screen-Shot-2024-06-07-at-10.58.35-AM.png,","
Ontholestes SP<\/em>\u00a0was identified using the SEEK app and taxonomic methods of identification. Wolbachia-rich sections of the beetle’s guts were removed from the abdomen by squeezing the abdomen. The DNA in this section was then extracted and eluted.<\/p>\n
The gel was divided into two parts: the upper section of the gel contained results to confirm or deny the presence of Arthropoda gene CO1, while the lower section confirmed and denies presence of Wolbachia genes. both sections used positive and negative controls, DNA controls, and water controls, most of which worked properly.<\/p>\n",No,GGAATAGTAGGAACTTCCCTCAGTCTACTTATTCGAGCCGAACTTGGAAACCCTGGAACCTTAATTGGTGATGATCAAATTTATAACGTTATTGTTACAGCTCATGCATTTGTAATAATTTTCTTCATGGTAATACCAATTGTTATTGGTGGATTTGGAAATTGACTAGTACCCTTAATACTAGGAGCTCCTGATATAGCTTTCCCTCGAATAAATAACATAAGATTTTGACTTTTACCCCCTTCTCTAACTCTTCTCTTAATAAGAAGAATAGCTGAAAGAGGGGCCGGAACTGGATGAACCGTTTACCCCCCTCTTTCAGCTAATGTGGCCCATAGAGGAGCTTCGGTTGATTTAGCTATTTTTAGATTACACTTAGCTGGTATCTCATCAATTCTTGGCGCAGTAAACTTTATTACTACAGTAATCAATATACGATCCACAGGAATAACATTTGATCGAATACCATTATTTGTTTGATCGGTAAGAATTACAGCTCTATTGCTTCTTTTATCCTTACCAGTTTTAGCAGGTGCTATCACTATGCTTTTAACTGATCGGAATTTAAATACAACATTTTTTGACCCTGCTGGTGGAGGAGACCCAATTCTTTATCAACATTTATTTTGATTTTTTGGTCACCCTGAAGTTTA,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/MD08-Premixededited.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/IMG_7520-rotated.jpg,",40.6204638667,-74.5698686851,06/03/2024,"
This arthropod was found near a fallen tree inside the forest at the Pingry Campus in Basking Ridge, New Jersey.<\/p>\n",Scolopocryptops sexspinosus,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Screenshot-2024-06-07-at-11.49.35 AM.png,","
The SEEK app was used to identify the insect’s species. The insect was crushed and used in its entirety. After identifying it, its DNA was extracted, eluted, run through PCR, and run through the gel.<\/p>\n
The top section of the gel is the control for the COI gene in arthropods, and the bottom section of the gel tests for Wolbachia amplification.<\/p>\n",Yes,TTGAGCATCAATAGCAGGAACTGCTCTTAGCCTAATTATCCGTTTAGAATTGAGTCAACCGGGAACCCTAATTGGTGATGATCAAACTTACAATACTATTGTAACTGCTCACGCATTTGTAATAATTTTCTTTATAGTAATACCAATTATAATTGGAGGATTCGGAAATTGATTAACCCCTTTAATACTAGGAGCTCCTGATATAGCTTTCCCTCGACTTAATAATATAAGTTTTTGATTACTTCCTCCTTCATTAATACTTTTAATAGGATCAGCTATAGTTGAAAGAGGAGCCGGAACAGGATGAACAGTATACCCTCCTCTAGCAGCTAATCTTGCACACTCAGGACCTTCAGTAGATATAACAATTTTTTCATTACATCTTGCTGGAGTTTCTTCTATTTTAGGAGCTATTAATTTTATTACAACTATTATTAATATACGAACAAGAGGAATAGTAATAGAACGAGTTCCCCTTTTTGTCTGGGCAGTATTAATCACCACCATCCTTCTTTTATTATCTCTCCCTGTATTAGCAGGAGCAATTACNATATTACTTACAGATCGAAATTTTAATACTAGATTTTTTGATCCAGCAGGAGGAGGGGATCCTATTTTATACCAACATTTATTTTGATTTTTG,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/MD18-Premixed-2.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/image_67541761.jpg,",40.6192066055,-74.5686656966,05/28/2024,"
We caught this Scolopocryptops sexspinosus<\/em> covered in leaves about 2 inches underground. This centipede was orange in color and approximately an inch in length. Scolopocryptops sexspinosus<\/em>, or the Eastern Red Centipede, is native to North America and is often found hiding under rotting wood or leaf litter.<\/p>\n",Scolopocryptops sexspinosus,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Screenshot-2024-06-07-10.20.23-AM.png,","
The centipede was identified using the SEEK app. After identification, the centipede’s DNA was extracted, and we performed PCR for Wolbachia 16s ribosome and Arthropod COI gene. After amplifying the DNA, we then ran the DNA through a gel electrophoresis system, and scanned the gel to confirm or deny the existence of Wolbachia DNA and Arthopod DNA in our sample. The gel results don’t have a band at 708bp for the Arthopod COI gene, but the identity of the bug is confirmed from the sequencing results.<\/p>\n",No,GCTTGAGCATCAATAGCAGGAACTGCTCTTAGCCTAATTATCCGTTTAGAATTGAGTCAACCGGGAACCCTAATTGGTGA TGATCAAACTTACAATACTATTGTAACTGCTCACGCATTTGTAATAATTTTCTTTATAGTAATACCAATTATAATTGGAG GATTCGGAAATTGATTAACCCCTTTAATACTAGGAGCTCCTGATATAGCTTTCCCTCGACTTAATAATATAAGTTTTTGA TTACTTCCTCCTTCATTAATACTTTTAATAGGATCAGCTATAGTTGAAAGAGGAGCCGGAACAGGATGAACAGTATACCC TCCTCTAGCAGCTAATCTTGCACACTCAGGACCTTCAGTAGATATAACAATTTTTTCATTACATCTTGCTGGAGTTTCTT CTATTTTAGGAGCTATTAATTTTATTACAACTATTATTAATATACGAACAAGAGGAATAGTAATAGAACGAGTTCCCCTT TTTGTCTGGGCAGTATTAATCACCACCATCCTTCTTTTATTATCTCTCCCTGTATTAGCAGGAGCAATTACAATATTACT TACAGATCGAAATTTTAATACTAGATTTTTTGATCCAGCAGGAGGAGGGGATCCTATTTTATACCAACATTTATTTTGAT TTTTTG,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/PAMD14-Premixed.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Sample-4-scaled.jpg,",46.9589244302,-95.1121343200,07/01/2022,"
Female<\/p>\n",Dermacentor variabilis,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,GelRed,"","",No,NNNAANCTTATTTTCGGCAGTTCGAGCAGNGAGTCACNAGGAATAAGAATAAGAATTTTAATTCGAATAGAATTAAGCCAACCAGGAACTTTAATTGGAAATGATCAAATTTATAACGTAATTGTAACTGCTCATGCATTTATTATAATTTTTTTCATAGTTATGCCTATCATAATTGGTGGGTTTGGAAACTGACTTGTACCATTCATATAAGGAGCCCCAGATATAGCATTTCCACGAATAAATAATATAAGATTTTGACTATCACCTCCATCATTATTTTTACTTATAANTTCTTCATTAATTGAATCAGGAGCAGGAACAGGTAGAACTGTTTATCCCCCTTTATCAACAAATTTGTCTCACTATGGACCTGCAGTTGATTCAGCTATTTTTAGCCTTCATCTAGCAGGTGCTTCCACTTTTTAAGGAGCGATTAATTTTATCACCACATTAATAAATAAACGATCAATTGGTTTAACTCTGGAACGAATACTTTTTTTTGTTTGAGCAGTTTTAATAACAGCTATTTTACTTCTTCTTTCTCTCCTTTTTTAAGCAGGAGCCATAACAATTTTATAAACAGACCGTAACTTTAATACTTCCTTTTTCNACCCTGCAGGGGGGGAAGACCCCTTTTTGTATCACCTTTTTTTTTTGNTTTTTTGGCACCAAAAAAAAATAAAAAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Sample-2-scaled.jpg,",46.9589830115,-95.1115039041,07/05/2023,"
Female<\/p>\n",Dermacentor variabilis,DNeasy (Qiagen) blood and tissue kit,,Standard electrophoresis system,TAE,GelRed,"","",No,GCACNCNANCTCGGAGTTGAGCAGGAGTCCAGGAATAAGAATAAGAATTTTAATTCGAATAGAATTAAGCCAACCAGGAACTTTAATTCGGAAATGATCAAATTTATAACGTAATTGTAACTGCCCATGCATTTATTATAATTTTTTTCATAGTTATGCCTATCATAATTGGTGGGTTTGGAAACTGACTTGTACCATTTANATAAGGAGCCCCAGATATAGCATTTCCACGAATAAATANTANAAGATTTTGACTTTNACCTCCTTCATTATTTTTACTTATAANTCCTTCATTAATTGAATCAGGAGCAGGAACAGGTTGAACTGTTTATCCCCNTTAATCACCAAATTTNTCTCATTATGGACCTTCAGTTGATTAAGCTATTTTAAGCCTTNATCTAGCAGGTGCTTCCANTATTTAAGGAGCAATTANTTTAATAACCACATTNTTAATTAAACGNCCAATTGGTTAAACTCTGGAACGAATACTTTAATTTGTTTGACCAGTTTAAATAACAGCAATTTTACTTCTCCTTTCTCTCCNTGTTTAAGCAGGAGCCATTACNATTTTTTAAACAGATCGAAACTTTAAAACTTCCTTTTTCGACCCTCCAGGGGGGGAAGACCCCTTTTTGTATCATNCATCTTTTTGGATTATTAAGCNNNNNNNNNNNNTAAAAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/IMG_2743-rotated.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Screen-Shot-2024-06-06-at-1.47.31-PM.png,",40.6199426000,-74.5681530000,06/03/2024,"
The Agonum punctiforme was discovered next to the pavement by a field of grass. It resembled a beetle at first, being black with a hard shell and two antennas. After sequencing the DNA and running the BLAST search, we can confirm that the insect is an Agonum punctiforme, with 548 base pairs matching that of the Agonum punctiforme. There were only 548 base pairs sequenced, so the match was exact.<\/p>\n",Agonum punctiforme,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Screen-Shot-2024-06-06-at-2.06.37-PM.png,","
Extracted the DNA, Purified the DNA, elution of DNA, then did PCR on the DNA separately for the arthropod and Wolbachia, ran a gel on both the arthropod and Wolbachia data, then purified the DNA to send over for sequencing the Wolbachia and arthropod DNA<\/p>\n
When doing PCR, there may have been a mistake in the PCR cocktail for Wolbachia. More Taq polymerase, water, and primers were added than necessary, so the proportions of the solution may have been inaccurate. There was still cocktail left over after pipetting the solution into each tube for PCR. There may have been contamination in the Wolbachia DNA as well, so the concentration was very low (only 1.4).<\/p>\n",No,TNNNNNTCGGAGCATGATCNGGAATAGTAGGGACTTCATTAAGTATATTAATTCGAGCTGAATTAGGAAATCCTGGAGCATTAATTGGAGATGACCAAATTTATAATGTTATTGTAACTGCTCATGCATTTATCATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGTTTTGGTAATTGATTAGTACCTTTAATATTAGGAGCACCTGATATAGCCTTTCCTCGAATAAATAATATAAGTTTTTGACTTCTTCCTCCTTCTTTAACACTACTCTTAATGAGAAGTATAGTAGAAAGAGGGGCTGGTACCGGATGAACAGTTTATCCACCTCTATCATCAGGTATCGCCCATGCCGGAGCTTCAGTTGATTTAGCAATTTTTAGTCTTCATTTAGCAGGAGTTTCATCAATTTTAGGGGCTGTAAATTTTATTACAACGATTATTAATATACGATCAGTAGGTATAACATTTGATCGAATGCCATTATTTGTTTGATCAGTAGGAATTACTGCTCTTCTATTGTTACTTTCTTTACCAGTACTAGCAGGAGCCATTACAATATTACTTACAGATCGAAATTTAAATACATCATTTTTCGACCCGGCAGGAGGAGGAGACCCAATTCTTTATCAACATTTATTTTGATTTTTTGGTCACCTGGAAAGTTTAA,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Arthro2Clean.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/waspy-1-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/wasp-pic.jpeg,",40.6200240000,-74.5680872000,05/31/2024,"
This arthropod was found walking by the roots of a tree on dry leaves at the edge of the forest at the Pingry Campus in Basking Ridge, New Jersey. The arthropod has the appearance of a wasp, with a stinger, 6 long legs, antennae, a segmented body, and two wings.<\/p>\n",Ichneumonidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Screenshot-2024-06-06-at-1.49.51-PM.png,","
The Ophion costatus <\/em>was found on 5\/31\/2024 and was placed in ethanol. Prior to extracting the DNA, I ran the arthropod underwater for two minutes and performed DNA precipitation, DNA purification, and DNA elution. After running the DNA through PCR to amplify both its own DNA and the DNA of Wolbachia, I ran gel electrophoresis.<\/p>\n
In my gel, the Arthropod section is on the top, and the Wolbachia is on the bottom. Each section contains (in order) the DNA ladder, the DNA from the respective arthropods of the three members of my group (mine is lane four), a positive Wolbachia control, a negative Wolbachia control, a DNA+ control, and a water control.<\/p>\n
<\/p>\n",No,TCAGTTTCCGTGCTATGTCCTTCGCTC,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/bug_MD05-4.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/P2210451-scaled.jpg,",33.6198251000,-86.6088756000,,"",Photinus pyralis,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Edvotek Gel Electrophoresis,TAE,GelRed,"","",No,GGCAATTTTNTCTTTGGGGCATGAGAGGAATACTAGGAACTTCATTCAGATTATTAATTCGTGCAGAATTAGGAATTCCGGGATCCTTAATTGGAAATGATCATATTTTTAATGTAATTGTTACTAGACACGCCTTCATTATAATTTTTTTTATAGTAATGCCTATCATAATTGGGGGCTTCGGGAATTGGTTAGTTCCCTTAATACTCGGGGCTCCTGATATAGCTTTTCCTCGAATAAACAATATAAGATTTTGATTATTACCCCCATCCCTATCTCTCTTAATTATAAGAAGAATAATTGAAAGAGGGGCAGGAACTGGCTGAACTGTCTACCCTCCCCTGTCAGCTAATATTGCTCACAGAGGTCCTTCAGTAGATTTAGCAATTTTTAGACTTCATCTTGCTGGGGTATCCTCAATTCTAGGTGCAGTAAACTTTATTTCAACTATTATTAATATACGGCCAAATAGAATAATATTAGATCAAATACCCTTATTTGTTTGAGCAGTTCTAATTACCGCAATTCTTTTATTATTATCATTACCCGTATTAGCAGGTGCTATTACTATACTCTTAACAGATCGAAATTTAAATTCTTCTTTTTTTGATCCGGCAGAAGGAGGAGATCCAGTTTTATACCAACACTTATTTTGATTTTTGGCACGCCAANNNNNNNAAA,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/bug-larva.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/other-pic.png,",40.7066174000,-74.5493284000,05/28/2024,"
This Misogada unicolor is in its larva stage. It is a red color and very small. It sat in alcohol for a few days before use so it was shriveled.<\/p>\n",Misogada unicolor,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/moth-larva-gel.png,","
The Misogada unicolor<\/em> was placed in alcohol where it sat for 2 days. Then it was run under water for two minutes to clean it. The entire Misogada unicolor<\/em> was crushed and then went through the steps of cell lysis and DNA precipitation. It was incubated for around an hour at 65 degrees C. Then the DNA was purified and eluted. After that the DNA concentration was checked. Then I started the PCR protocol. PCR was run. After PCR, I mixed a gel out of agarose, SYBER safe and running buffer. The gel was loaded and ran in a electrophoresis chamber. We read the gel for results.\u00a0 Then I did PCR DNA purification to send to the lab for sequencing.<\/p>\n",Yes,AGCTGGAATAGTAGGAACTTCATTAAGATTATTAATTCGAGCAGAATTAGGAAATCCTGGATCACTAATCGGAGATGACCAAATTTACAATACTATTGTAACAGCCCATGCATTTATCATAATTTTTTTTATAGTAATACCAATTATAATTGGGGGATTTGGAAATTGATTAGTACCTTTAATACTCGGGGCCCCTGATATAGCATTCCCACGAATAAATAATATAAGATTTTGACTTTTACCTCCTTCTATTACTCTTTTAATTTCTAGAAGAATCGTATAAAATGGGGCTGGAACTGGATGAACAGTTTACCCACCTTTATCATCTAATATTGCTCATGGGGGTAGATCAGTAGATTTAGCTATTTTTTCGCTTCATTTAGCTGGTATTTCATCTATTTTAGGAGCTATTAATTTTATTACTACAATTATTAATATACGATTAAATAATTTATCATTTGATCAAATACCTTTATTTGTGTGAGCTGTANGTATTACTGCCTTTCTTTTATTATTATCTTTACCTGTTTTAGCANGAGCTATTACTATATTATTAACAGACCGAAATTTAAATACTTCCTTTTTTGATCCTGCCGGAGGAGGAGACCCAATTTTATACCAACATTTATTTTGATTTTTTGGTCACCCTGAAGTTTAN,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/arthro-1-FASTA.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/mealworm-png.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/mealworm.png,",40.7066174000,-74.5493284000,05/29/2024,"
This Tenebrio molitor<\/em> is a black mealworm beetle. It was grown in a lab where it was bred to eat styrofoam. It was approximately 3 mm. The Tenebrio molitor<\/em>\u00a0has antennas and 6 legs which is consistent with insecta.<\/p>\n",Tenebrio molitor,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/mealworm-1.png,","
After capture, the Tenebrio molitor<\/em> was placed in alcohol. Prior to DNA extraction, the Tenebrio molitor <\/em>was run under water for two minutes to clean it. Then it was dissected and the abdomen was kept. The abdomen was crushed up in 1.5 tube using a pestle. Next we followed the process of the DNeasy extraction kit. After the extraction, the DNA concentration was checked. With the extracted DNA, we ran PCR.\u00a0 After PCR, I mixed a gel out of agarose, SYBER safe and running buffer. The gel was loaded and ran in a electrophoresis chamber. Then I did PCR DNA purification to send\u00a0 out for sequencing.<\/p>\n",No,TAGTCGGAACCTCTCTTAAGACTATTAATCCGTGCAGAATTAGGAAACCCCGGCTCTTTAATTGGGGACGACCAAATCTTACAACGTAATTGTTACAGCACATGCTTTTATCATAATTTTTTTTATAGTAATACCAATCATAATTGGAGGATTCGGAAATTGATTTTT,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/MD02-Premixed-FASTA.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/IMG_5155-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/IMG_7508-scaled.jpg,",40.6200000000,-74.5690000000,06/03/2024,"
The Camponotus chromaiodes\u00a0<\/em>was found on a dry, dusty, sunlit part of the path into the forest. The ground it was found on was littered with cracks and holes where many similar ants of its species were ducking in and out. The Camponotus chromaiodes\u00a0<\/em>was extremely fast and seemed to be a bit smaller for its kind, indicating that it wasn’t fully developed. Upon further observation under a microscope, we could see that the Camponotus chromaiodes <\/em>had a smaller abdomen and very large antennae with a distinctly rusty color along its mesosoma and legs.<\/p>\n",Camponotus chromaiodes,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Image-6-6-24-at-11.44 AM.jpg,","
In order to begin our DNA extraction, we had to dissect the abdomen of the Camponotus chromaiodes<\/em> and wash it under running water for two minutes to remove the ethanol present. We went through the steps of cell lysis and precipitation, DNA purification, and DNA elution. After that, we checked our concentrations with a nanodrop and finished PCR while setting up our gel. After about 15-20 minutes, we checked on our gel and visualized our results (see gel above). My arthropod was in Lane 3. The top lane contained our Arthropod samples, and the bottom lane contained our Wolbachia samples, which helped us determine that our bug contained Wolbachia (see gel above).<\/p>\n",Yes,GTTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTGGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTAATTCCCCTTATACTAGGATCTCCTGATATAGCTTACCCCCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGGACTGTCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATCAATTTTATTT,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/MD05-Premixed-2-1.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Screen-Shot-2024-06-06-at-11.00.35-AM.png,",40.6204456142,-74.5698992217,06/03/2024,"
Slightly-red tint on abdomen, very long antennae, ant-like motion<\/p>\n
caught in a tree in a forest outside of the Pingry school.<\/p>\n
Camponotus nearcticus- neartic carpenter ant<\/p>\n
<\/p>\n",Formicidae,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Screen-Shot-2024-06-07-at-10.37.53-AM.png,","
missing arthropod DNA due to unsuccessful sequencing<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_0055-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Screen-Shot-2024-06-06-at-1.54.25-PM.png,",40.6208381739,-74.5701465032,05/28/2024,"
The Nomada sp. has two striped lines (yellow and black) on its abdomen, a thorax, and wings. There appears to be a stinger of sorts on its head as well, which was confirmed by the BLAST results of sequencing the DNA. The physical descriptions match the other insects in the genus Nomada. In the forest, it was found next to a small tree with a lot of sunlight.<\/p>\n",Nomada,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Screen-Shot-2024-06-06-at-1.56.15-PM.png,","
Extracted the DNA, Purified the DNA, elution of DNA, then did PCR on the DNA separately for the arthropod and Wolbachia, ran a gel on both the arthropod and Wolbachia data, then purified the DNA to send over for sequencing the Wolbachia and arthropod DNA<\/p>\n",Yes,TCGNTNNATGGTCNGGAATAATTGGAACTTCTATAAGATTTATTATTCGTATAGAATTAAGAAACCCCGGAGAATGAATTATAAACGATCAAATTTATAATTCTTTAGTTACAGCTCATGCCTTTTTAATAATTTTTTTTATAGTTATACCTTTCATAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGATGTCCTGATATAGCTTTTCCTCGAATAAATAATATTAGATTCTGATTATTACCTCCTTCTTTAATTTTATTATTATTAAGGAATCTATTTAATATTACCCCAGGAACAGGATGAACAGTCTATCCTCCATTATCTTCATCAATATTTCATAGATCACCATCAGTTGATTTAGCAATTTTTTCTCTTCATATATCAGGAATTTCTTCAATTATAGGAGCAATAAATTTTATAGTTACAATTATATTAATAAAAAATATTTCAATAAATTATGATCAAATTAATTTATTTTCATGATCAATTTTTATTACTGCTCTTTTATTATTACTATCTTTACCAGTATTAGCTGGAGCAATTACTATATTATTATTTGATCGAAATTTTAATACTTCATTTTTTGACCCTATAGGAGGAGGAGATCCAATTTTATATCAACATTTATTTTGATTTTTTGGTCACCCTGGAAGTTTAA,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/ArthroClean.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/original-1.jpeg,",40.6198161496,-74.5706966588,05/28/2024,"
Note about photograph: unable to capture a clearer photo, as the spider moved very fast. Spider is the silver outer shell within the jar.<\/p>\n
Spider was captured in the first outside a large building, on top of a bed of moss.<\/p>\n
<\/p>\n
Leucauge venusta<\/em>– orchard orbweaver<\/div>\n",Cyclopidae,DNeasy (Qiagen) blood and tissue kit,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Screen-Shot-2024-06-07-at-10.56.32-AM.png,","
Although there was a PCR fragment, we did not get good arthropod sequence results.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/IMG_5410-scaled.jpg,",40.6199362000,-74.5681621000,05/28/2024,"
I began by finding a common fly in the wild. From here I captured the bug and placed it in a tube of ethanol. I then crushed the bug to prepare to perform DNA Extraction. Followed by PCR, Gel Electrophoresis, and PCR Purification.<\/p>\n",Musca domestica,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Image-6-6-24-at-1.32 PM.jpg,","
The protocol ran smoothly. All controls produced sensible results and the arthropod samples produced results similar to what is expected.<\/p>\n",Yes,TGGAGCATGAGCAGGAATAATTGGTACTTCATTAAGAATTTTAATTCGAGCAGAATTAGGACATTCTGGAGCTTTAATTGGTGATGACCAAATTTATAATGTAATTGTAACAGCTCACGCTTTTATTATAATTTTTTTTATAGTTATACCAATTATAATTGGAGGATTTGGTAATTGATTAGTTCCTCTTATACTAGGAGCTCCTGATATAGCTTTCCCTCGAATAAACAATATAAGATTTTGACTTCTTCCTCCTGCACTAACTTTACTATTAATAAGTACATTAATAGAAAATGGAGCTGGAACAGGATGAACTGTTTATCCTCCCTTATCATCTAATATTGCTCATAGAGGACCTTCTGTAGATTTAACTATTTTTTCTCTTCATTTAGCTGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACAACAACTATTAATATGCGTTCAACAGGTATTACTTTTGATCGAATACCTTTATTTGTTTGATCAGTATTAATTACAGCTATTCTTTTATTACTTTCTTTACCAGTTCTTGCAGGAGCAATTACTATATTATTAACTGATCGAAATATAAATACTTCCTTTTTTGACCCAGCAGGAGGAGGAGATCCTATTCTATACCAACACTTATTTTGATTTTTTGG,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/MD03-Premixed.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Screenshot-2024-06-06-at-2.12.48 PM.png,",40.6200274500,-74.5701961000,05/31/2024,"
The Camponotus<\/em> seemed to be attracted to the fallen tree. I believe a colony is most likely nearby due to the high number of Camponotus<\/em> on the log.<\/p>\n",Camponotus chromaiodes,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Screenshot-2024-06-07-at-11.07.50 AM.png,","
Insect trapping, DNA extraction, DNA amplification, gel electrophoresis, DNA sequencing and analysis<\/p>\n",Yes,NNNNNNNNNNTCTTTGCTATTTGATCGGGANTAGTTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTGGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTAATTCCCCTTATACTAGGATCTCCTGATATAGCTTACCCCCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGGACTGTCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTAGTATGGTCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGAGCTATTACAATACTACTAACAGACCGAAATCTTAATACTTCATTTTTCGACCCTTCGGGAGGGGGAGATCCTATTTTATACCAACATTTATTTTGATTTTNNNNNCNNNAAAANTTAAA,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/MD02-Arhropod-Ant.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/IMG_6738-1-scaled.jpeg,",40.6202900000,-74.5704400000,05/28/2024,"
Has a stinger, seems to be a type of wasp, lives in a terrestrial habitat<\/p>\n",Conura,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/GEL-KG.png,","
DNA extraction (Cell Lysis, Purification, Elution), PCR, Gel Electrophoresis, PCR Purification, Sequencing<\/p>\n",Yes,TTTACGNTNCGAATTCGTTTCNTTAGNGCTNATNNAGNNGGNTTTNCGNTTGACNNCTTTAAANCTNNNANGTNACGTTGCANNGNNTAGCGGTAGCCTGTTAACCAANNNGGTNTCTCNNCGCTGATCAAAAGNTNTTCCNATNNAATCNATCNGNNTAGNNATGACTNTGCGNNCGNGTNCNANAATACACTNTGAGNNATGAGAGCCTNAAGGAACANGATTTTTATCNATACTTGGTATCTCNCNACCN,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/2024-BB-Arthropod-KG-.fa,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/04/Pardosa-amentata-oben.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/04/Pardosa-amentata-unten.jpg,",47.4591489978,9.0228409901,03/14/2024,"
This probe was found under dead wood. It was found alone, without further population.<\/p>\n",Pardosa amentata,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/04/Gel1-1.jpg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/04/oniscus-asellus-oben-2.jpg,",47.4595771420,9.0231698102,03/14/2024,"
The tested probe was found under dead wood. It was taken from a whole population.<\/p>\n",Oniscus asellus,Instagene Matrix,Abdomen,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/04/Gel1-1.jpg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/04/Forficula-auricularia-1-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/04/Forficula-auricularia-2-scaled.jpg,",47.4597869835,9.0190839886,03/07/2024,"
Found on a tree trunk. It was collected in the morning when it was 6 degrees celsius and in cloudy weather.<\/p>\n",Forficula auricularia,Instagene Matrix,Rear half,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/04/Gelelektrophorese-5.jpg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/04/Oniscus-asellus-1-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/04/Oniscus-asellus-2-1.jpg,",47.4603524355,9.0179671681,03/07/2024,"
I found this insect under a dead tree. It was collected in the morning when it was 6 degrees celsius and in cloudy weather.<\/p>\n",Oniscus asellus,Instagene Matrix,Rear half,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/04/Gelelektrophorese-4.jpg,","",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Centipede-DNA-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Stink-Bug-DNA-scaled.jpeg,",43.7454609000,-87.7945246000,01/18/2024,"
Lane 4 Centipede DNA-Wolbachia Positive (Very faint in image)<\/p>\n
Land 8 Stink Bug DNA-Wolbachia Positive (Very Faint in Image)<\/p>\n",Arthropoda,pcr,Abdomen,MiniOne,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Arthopod-DNA-3-scaled.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Wolbachia-DNA-scaled.jpeg,","
1-DNA Ladder, 2-DNA Ladder, 3-Water, 4-Centipede, 5-Arthropod Control (-), 6-Arthropod Control (+), 7-DNA Control (+), 8-Stink Bug<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/1000008135-scaled.jpg,",43.7398656325,-87.7765836390,11/30/2023,"
I noticed that it was big enough to cut its abdomen to get the DNA, so I caught it in hopes of getting Wolbachia.<\/p>\n",Rhopalidae,tweezers,Abdomen,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/1000008984.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/1000008983.jpg,","
Took about 3 class periods ,so 5 and a half hours First image- Wolbachia. ORDER OF SEQUENCE: Dna Ladder, Hoveryfly DNA, BOXELDER BUF DNA, positive arthropod control, negative arthropod control, postive DNA control, Water. Second Image- ORDER OF SEQUENCE:Hoverfly Dna, Boxelder Dna, positive arthropod control, negative arthropod control, postive DNA control, Water, Dna Ladder <\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/IMG_5179.jpeg,",43.75785497486573,-87.70651926988052,11/10/2023,"
It looked like a bee<\/p>\n
Looked weak when caught, most likely due to cold weather<\/p>\n
Found on doorbell<\/p>\n",Syrphidae,tweezers,Gonads dissected,MiniOne,TBE,GelGreen,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/arthropod.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/wolbachia.jpg,","
Took about three class periods (5 and a half hours)<\/p>\n
Arthropod Image:<\/p>\n
Well 1: Hoverfly<\/p>\n
Well 2: Boxelder Bug<\/p>\n
Well 3: Positive Arthropod control<\/p>\n
Well 4: Negative Arthropod control<\/p>\n
Well 5: Positive DNA control<\/p>\n
Well 6: Water<\/p>\n
Well: DNA Ladder<\/p>\n
<\/p>\n
Wolbachia Image:<\/p>\n
Well 1: DNA Ladder<\/p>\n
Well 2: Hoverfly<\/p>\n
Well 3: Boxelder bug<\/p>\n
Well 4: Positive Arthropod Control<\/p>\n
Well 5: Negative Arthropod Control<\/p>\n
Well 6: Positive DNA Control<\/p>\n
Well 7: Water<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_3102-1.jpg,",43.7460157556,-87.7881311333,12/13/2023,"
Long proboscis<\/p>\n",Arthropoda,Qiagen Blood and Tissue kit,Whole arthropod,MiniOne,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/IMG_3147-2.jpg,","
Two ladders used. One is a benched up 100 BP ladder from ProMega. The other one is HindIII Lambda digest DNA standard.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_3101-1.jpg,",43.7635764013,-87.7875901204,,"
Curled up and smaller in stature<\/p>\n",Arthropoda,Qiagen Blood and Tissue kit,Whole arthropod,MiniOne,TBE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/IMG_7876-4.jpg,","
Two ladders used. One is a benched up 100 BP ladder from ProMega. The other one is HindIII Lambda digest DNA standard.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Beetle.png,",43.7651532934,-87.7554702569,04/09/2024,"",Arthropoda,pcr,Gonads dissected,MiniOne,LB,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Wolbachia.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Arthropod.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/BeePhoto.png,",43.7622773596,-87.7697891755,12/28/2024,"",,pcr,Gonads dissected,MiniOne,LB,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Wolbachia.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/Arthropod.png,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/FullSizeRender.jpg,",40.6867556000,-74.5580454000,05/28/2024,"
Black body, red abdomen and legs<\/p>\n",Camponotus chromaiodes,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,SYBR Safe,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/PNG-image.png,","
Protocol ran smoothly. The second ladder (1st after ladder) is the C. chromaiodes.<\/p>\n",Yes,CTTTGCTATTTGATCGNNANNGTTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTGGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTAATTCCCCTTATACTAGGATCTCCTGATATAGCTTACCCCCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGGACTGTCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGG,https://wolbachiaprojectdb.org/wp-content/uploads/2024/06/MD05-Premixed-FASTA.fa,, "",39.9850891000,-75.2620692000,09/11/2023,"",Aedes albopictus,,,,,,"","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Montana-Halictidae-scaled.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Montana-Halictidae-up-close-scaled.jpg,",45.5885220000,-111.5782930000,08/04/2021,"",Halictidae,,,,,,"","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/KZU_051_Flohkaefer-scaled.jpeg,",47.5181829121,8.5469813953,08/25/2023,"",Coleoptera,Instagene Matrix,Abdomen,MiniOne,TBE,Midori 1:20'000,"","
non gel image available<\/p>\n",Yes,AGTGGGAACTTCAATAAGAATTTTAATTCGAGCAGAATTAGGAAGCCCAGGATCCTTAATTGGAAATGATCAAATTTATA ATGTAATTGTTACTGCTCATGCATTTGTAATAATTTTTTTTATAGTTATACCAATTATAATTGGTGGATTTGGAAATTGA TTAGTTCCATTAATAATTGGAGCTCCTGATATAGCATTCCCACGAATAAATAATATAAGATTCTGGCTTTTACCCCCCTC TTTATTTTTATTACTAATAAGAAGATTAGTAGAAAGAGGAGCTGGAACAGGATGAACAGTTTACCCCCCACTTTCATCAA ATATTGCTCATGGTGGATCTTCAGTTGATCTCGCAATTTTTAGACTCCATTTAGCAGGAGTTTCTTCTATTTTAGGAGCA ATTAATTTTATTACAACAATTATTAATATACGACCTCAAGGAATATCTTTTGATCAAATACCCTTATTTGTATGGGCAGT ACTAATTACTGCTATTCTTTTACTTTTATCACTACCAGTTTTAGCTGGAGCTATTACAATACTTTTAACTGACCGAAATT TAAATACATCATTTTTTGACCCAATTGGTGGGGGAGATCCTATCCTATACCAACATTTATTTTGATTTTTTG,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/KZU_051A-CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/KZU_045_Hausspinne.jpg,",47.5180090130,8.5491700778,08/25/2023,"",Agelenidae,Instagene Matrix,Partial abdomen,MiniOne,TBE,Midori 1:20'000,"","
no gel image available<\/p>\n",No,TATAGTAGGGACTGCTATAAGAGTTTTAATTCGAATTGAATTAGGTCAATCTGGAAGATTTTTAGGAGATGATCAGTTAT ACAATGTTATTGTTACTGCTCATGCATTTGTTATAATTTTTTTTATAGTGATACCAATTTTAATTGGTGGATTCGGTAAT TGGTTGGTTCCTTTAATGTTAGGAGCTCCAGATATGGCTTTTCCTCGGATAAATAATTTAAGTTTTTGACTTCTTCCCCC CTCTCTTTTTATATTATTTATTTCTTCTATAGTAGATATAGGGGTTGGGGCAGGATGAACTGTTTATCCTCCTTTAGCTT CTTTAGAAGGACACCCAGGTAGATCTGTTGATTTTGCTATTTTTTCTCTTCATTTAGCTGGTGCTTCTTCTATTATAGGG GCTATTAATTTTATTTCCACTATTATTAATATACGAATAAGAGGAATTTCTATAGAAAAGGTCCCACTTTTTGTGTGATC TGTCCTAATTACTGCTGTTTTATTACTATTATCTTTACCTGTTTTAGCAGGTGCTATCACAATATTGTTAACGGATCGAA ATTTTAATACTTCTTTTTTTGATCCTTCTGGAGGAGGGGATCCAGTGTTGTTTCAACATTTATTTTGATTTT,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/KZU_045A-CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/KZU_044_Kellerassel.jpg,",47.5186611318,8.5476036677,08/25/2023,"
under decaying wood blocks<\/p>\n",Porcellio,Instagene Matrix,Abdomen,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/HCO-LCO-PCR-2023-12-07_Gel-A.jpeg,","
sample 44 (last lane) stems from Porcellio scaber<\/p>\n",Yes,NNNNCNGGGGCTNNNNGGNNNNCATTNANTATACTTATNCNGGCNGAGTTNNNNNNNCCCGGAGGGTTGATTGGGAGAGA TCAAATTTATAATGTAATTGTTACTGNNCATGCTTTTGTTATAATTNNNNTTATNNTAATACNNATTATANNNNNGGGGT TTGGTAATTGATTANTTCCTTTAATATTAGGGGCCCCAGATATNNCTTTTCCTCGTATGAATAANATANNNTTTTGATTG CTTCCNCCTTCCCTAATACTGCTTCTTANAANAGGATTAGTAGAANNANGGGTAGGAACAGGGTGGACAGTATATCCNCC TTTGGCANCNNNAATTGCNCATANNGGAGCCTCTGTAGATTTAGGAATTTTTTCMTTACATCTTGCAGGGGCNTCTTCNA TTTTAGGGGCTGTAAATTTTATTACTACNATCATTAATATACNGGCNNNNGGGATAANAATANACCGGGTGCCTTTGTTT GTATGGTCTGTAATAATTACNGCANTNNTTTNNNTTNNNNNNTTNCCTGTTTTGNCAGGGGCTATTACTATACTTCNNAC NNANCNAAATTNANNNNNNTCNTTTTNTNNTCCNGGNGGGGGAGGNNNNCCNATTNNTNNNCNNCNNCTNNTTTGATTTT TTG,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/KZU_044A-CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/KZU_047_Wiesen-Erdzikade_AlinaVittoria.png,",47.5183423192,8.5489125858,08/25/2023,"
resident of our school\u2019s meadow
\ndt Wiesen Erdzikade https:\/\/de.wikipedia.org\/wiki\/Wiesen-Erdzikade<\/a><\/p>\n",Cicadellidae,Instagene Matrix,Whole arthropod,MiniOne,TBE,Midori 1:20'000,"","
no gel image available<\/p>\n",No,GATGCTTAGATTTATTATTCGTATTGAACTTTCACAACCAGGTTCATTTTTGGGGAATGACCAAATTTATAATGTAGTTG TTACTTCTCATGCATTTGTAATGATTTTTTTTATAGTTATGCCTATTATAATTGGCGGGTTTGGAAATTGACTTGTTCCA CTAATATTAGGTGCTCCTGATATAGCATTTCCTCGAATAAATAATATGAGATTTTGGTTATTGCCTCCGTCATTAATTTT ATTATTAATGAGATCAATTGTTGAAATAGGTTCTGGAACTGGTTGAACTGTTTATCCACCCCTATCTTCCAATATTTCCC ACTCTGGTCCTAGAGTAGATTTAACTATTTTTTCTTTACATTTGGCTGGTATTTCATCTATTCTTGGGGCTATTAATTTT ATTTCAACTATTATTAATATGCGAATTCAGGGCATAAATATAGATAAAATACCTTTATTTGTTTGATCAGTTTTTGTTAC GGCTATTCTTTTAATGCTTTCATTACCTGTTTTAGCAGGAGCTATTACTATACTATTAACAGATCGTAATTTAAATACAA CCTTTTTTGACCCTTCAGGTGGAGGGGATCCTATTTTGTATCAACATTTATTTTGATTTTTTG,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/KZU_007A-CO1F-1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/KZU_007_BernsteinWaldschabe.png,",47.5187335890,8.5477538715,08/25/2023,"
there were multiple specimens of Ectobius v.<\/p>\n",,Instagene Matrix,Partial abdomen,,TBE,Midori 1:20'000,"","
gel image not (no more) available<\/p>\n",No,GGAACTTCTCTAAGTATACTAATTCGGACTGAATTAAATCAACCAGGATCTTTAATTGGAGATGATCAAATTTATAATGT TATTGTTACAGCTCACGCATTTATTATAATTTTTTTTATAGTTATACCCATTTTAATTGGAGGATTTGGAAATTGATTAG TACCTTTAATATTAGGAGCACCAGATATAGCCTTCCCACGAATAAATAATATAAGATTTTGATTATTACCACCATCCCTA ACACTTCTTCTCATGAGAAGTTTAGTAGAAAGAGGGGCAGGAACAGGATGAACAGTTTACCCTCCCTTAGCTAGAGGTAT TGCACATGCAGGAGCATCTGTTGATTTAGCAATTTTCTCATTACATCTTGCTGGAGTATCTTCTATTCTAGGAGCAGTAA ATTTTATTTCCACAATTATTAATATAAAACCTATCAATATAACTTCAGAACAAATTCCTCTCTTCGTTTGATCTGTAGGT ATTACTGCCTTATTATTATTATTATCTTTACCGGTATTAGCTGGTGCTATTACAATATTATTAACAGATCGCAATCTCAA TACATCATTTTTTGATCCCGCAGGAGGAGGTGATCCCATCCTATATCAACATTTATTTTGATTTTTTGG,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/KZU_007A-CO1F.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Halictidae-from-Wolbachia-project.png,",39.9850891000,-75.2620692000,11/03/2022,"
We achieved Wolbachia positive PCR results using three primer pairs: a LepF primer pair, a Wsp (Wolbachia surface protein) primer pair, and a W-spec (Wolbachia specific) 16S primer pair. The 16S sequence we received from the W-spec primer pair PCR matched the sequence from a 16S Wolbachia PCR in Ethiopia.<\/p>\n",Halictidae,,,,,,"","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Andrena-with-Wolbachia-1.png,",39.9850891000,-75.2620692000,06/01/2023,"",Andrena,,,,,,"","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/pharaoh-ant.jpg,",33.8610309000,-118.2547536000,05/13/2024,"
Found in the CAMS parking lot, surrounded by several other ants, light brown color, appeared more transparent with a brighter light<\/p>\n",Insecta,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_0833.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_0834.png,","
Ran the gel for 60 minutes at 105 volts.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/black-garden-ant.jpg,",33.8610309000,-118.2547536000,05/14/2024,"
Found near the soil of the garden, dark brown\/black color, around a few other ants<\/p>\n",Insecta,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_0833.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_0834.png,","
Ran the gel for 60 minutes at 105 volts.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/carpenter-ant.jpg,",33.8610309000,-118.2547536000,05/13/2024,"
Found on a tree at CAMS, active, yellow stripes on abdomen, brown color, shaded area<\/p>\n",Insecta,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_0834.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_0833.png,","
Ran the gel for 60 minutes at 105 volts.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_2168-preview.jpg,",33.8592989254,-118.2556799170,05/21/2024,"
Spider with 8, clear-like legs. Dark brown bulb with cream-colored spots. Size of a grain of rice.<\/p>\n
Caught in the bush of the lowest parking lot, with forceps and petri dishes.<\/p>\n",Oecobius navus,tweezers,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_5713-scaled.jpeg,","
100 volt, 60+ minutes<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_2177.jpeg,",33.8594260868,-118.2557041184,05/20/2024,"
The ant was smaller than a grain of rice and was a black\/brown color all the way through. Found in dirt with other ants.<\/p>\n",Formicidae,tweezers,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_5713-scaled.jpeg,","
100 volt, 60+ minutes<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_2721-scaled.jpg,",33.8663957000,-118.2538329000,05/14/2024,"
Found in a shady area with bushes, crawling away. Evaded capture multiple times.<\/p>\n",Arachnida,tweezers,Abdomen,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/WolbachiaGel-1.png,","
Gel ran for up to an hour. Visualized immediately after using UV light.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_2719-scaled.jpg,",33.8541178000,-118.1832928000,05/14/2024,"
Arthropod was caught on a raspberry, left as a trap, and put into freezer to slow it down.<\/p>\n",Diptera,tweezers,Whole arthropod,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/wolbachia-2.png,","
Ran for about an hour at 125V and visualized immediately after (avoid UV dissipating) using UV light.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_2712-scaled.jpg,",33.8973190000,-118.2580231000,05/14/2024,"
Squirming behind laundry basket with lots of dust.<\/p>\n",Insecta,tweezers,Abdomen,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/WolbachiaGel.png,","
Gel was ran for close to an hour. Visualized immediately after using UV light.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Screenshot-2024-05-24-at-9.44.10 PM.png,",,,,"
Arthropod was captured in the school garden infront of the classroom on one of the plant leaves.<\/p>\n",Arthropoda,tweezers,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/248DA36A-2F7D-4965-88C7-D61432AF79C6-1.jpg,","
The gel ran for about 35 minutes at 135 volts and 70 amps. Results were viewed using a white light box.<\/p>\n
Lane 1: DNA Ladder<\/p>\n
Lane 2: Spotted ladybug DNA<\/p>\n
Lane 3: No spots ladybug DINA<\/p>\n
Lane 4: (+) Arthropod Control<\/p>\n
Lane 5: (-) Arthropod Control<\/p>\n
Lane 6: DNA Control<\/p>\n
Lane 7: Water<\/p>\n
<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/LadybugLarvae.png,",33.8620071372,-118.2552134924,05/14/2024,"
\n
Alone<\/li>\n
In dry land<\/li>\n
Shady areas filled with bushes<\/li>\n<\/ul>\n",Coccinellidae,tweezers,Whole arthropod,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/WolbachiaGel1.png,","",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_6438.jpg,",33.8606708925,-118.2527481366,05/15/2024,"
Rolypoly caught under a broken piece of wood logs just outside of CAMS parking lot.<\/p>\n",Armadillidium vulgare,tweezers,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"","
130 Volts, 60 Minutes<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_6422.jpg,",33.8606708920,-118.2527481000,05/15/2024,"
Rolypoly caught under a broken piece of wood logs just outside of CAMS parking lot.<\/p>\n",Armadillidium vulgare,tweezers,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"","
130 Volts, 60 Minutes<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_6412.jpg,",33.8606708920,-118.2527481000,05/15/2024,"
Rolypoly caught under a broken piece of wood logs just outside of CAMS parking lot.<\/p>\n",Armadillidium vulgare,tweezers,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"","
130 Volts, 60 Minutes<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_6403.jpg,",33.8606708920,-118.2527481000,05/15/2024,"
Rolypoly caught under a broken piece of wood logs just outside of CAMS parking lot.<\/p>\n",Armadillidium vulgare,tweezers,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"","
130 Volts, 60 Minutes<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Screenshot-2024-05-24-213051.png,",33.8604261297459,-118.25555395533287,05/15/2024,"
\u00a0Found on the dry ground of the vegetation next to the parking structure.<\/span><\/p>\n",Formicidae,tweezers,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Screenshot-2024-05-24-210706.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Screenshot-2024-05-24-210649.png,","
Both gels ran for 35 minutes at 135 volts and 70 amps. Results were viewed w\/ a white light box.\u00a0<\/span><\/p>\n
<\/p>\n
*Wells starting new ruler (Gel 1: Arthropod)<\/span><\/p>\n
Lane 1: DNA Ladder<\/span><\/p>\n
Lane 2: Aphids DNA<\/span><\/p>\n
Lane 3: Ants DINA<\/span><\/p>\n
Lane 4: (+) Arthropod Control<\/span><\/p>\n
Lane 5: (-) Arthropod Control<\/span><\/p>\n
Lane 6: DNA Control<\/span><\/p>\n
Lane 7: Water<\/span><\/p>\n
<\/p>\n
*Wells starting new ruler (Gel 2: Wolbachia)<\/span><\/p>\n
Lane 1: DNA Ladder<\/span><\/p>\n
Lane 2: Aphids DNA\u00a0<\/span><\/p>\n
Lane 3: Ants DINA<\/span><\/p>\n
Lane 4: (+) Wolbachia Control<\/span><\/p>\n
Lane 5: (-) Wolbachia Control<\/span><\/p>\n
Lane 6: DNA Control<\/span><\/p>\n
Lane 7: Water<\/span><\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Screenshot-2024-05-24-205529.png,",33.8607602412,-118.2551725058,05/15/2024,"
Arthropod was found in the school garden in clusters on the stems of milkweed in front of classroom.<\/span><\/p>\n",Aphididae,tweezers,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Screenshot-2024-05-24-210706.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Screenshot-2024-05-24-210649.png,","
Both gels ran for 35 minutes at 135 volts and 70 amps. Results were viewed w\/ a white light box.<\/span><\/p>\n
*Wells starting new ruler (Gel 1: Arthropod)<\/span><\/p>\n
Lane 1: DNA Ladder<\/span><\/p>\n
Lane 2: Aphids DNA<\/span><\/p>\n
Lane 3: Ants DINA<\/span><\/p>\n
Lane 4: (+) Arthropod Control<\/span><\/p>\n
Lane 5: (-) Arthropod Control<\/span><\/p>\n
Lane 6: DNA Control<\/span><\/p>\n
Lane 7: Water<\/span><\/p>\n
<\/p>\n
*Wells starting new ruler (Gel 2: Wolbachia)<\/span><\/p>\n
Lane 1: DNA Ladder<\/span><\/p>\n
Lane 2: Aphids DNA<\/span><\/p>\n
Lane 3: Ants DINA<\/span><\/p>\n
Lane 4: (+) Wolbachia Control<\/span><\/p>\n
Lane 5: (-) Wolbachia Control<\/span><\/p>\n
Lane 6: DNA Control<\/span><\/p>\n
Lane 7: Water <\/span><\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_0664-scaled.jpeg,",33.8649439077,-118.1803727918,05/22/2024,"
Small fly, attracted to fruit but was caught by trapping it in a bag with dog feces.<\/p>\n",Drosophilidae,none,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_0693-scaled.jpeg,","
100 Volts, 90 minutes<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_0665-scaled.jpeg,",33.9010683091,-118.3429826609,05/22/2024,"
Came toward the smell of banana peel and day-old eaten apple.\u00a0 Very large fly, buzzed very loud, and was seemingly easy to catch with petri dish.<\/p>\n",Drosophilidae,none,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_0693-scaled.jpeg,","
100 volt, 90 minutes.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_4466-scaled.jpg,",33.8624581000,-118.2544108000,05/14/2024,"
Arthropod caught on tree by lunch benches at CAMS<\/p>\n",Dilophus,tweezers,Abdomen,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_1314.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Gel.jpeg,","
Both gels ran for just over an hour, before being examined for bands<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_4464.jpg,",33.8624581000,-118.2544108000,05/14/2024,"
Caught just outside entrance, by sewer of CAMS<\/p>\n",Drosophila melanogaster,tweezers,Abdomen,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Gel.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_1314.jpg,","
Both gels ran for just over an hour, before being examined for bands<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_7188-1.jpg,",33.8624581000,-118.2544108000,05/14/2024,"
Ant caught on cement just outside entrance of CAMS<\/p>\n",Linepithema humile,tweezers,Abdomen,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Gel.jpeg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_1314.jpg,","
Both gels ran for just over an hour, before being examined for bands<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Screenshot-2024-05-24-at-1.28.08-PM.png,",33.8609901000,-118.2542238000,05/14/2024,"
Wildcaught. Caught near Pogonomyrmex barbatus, black colored, crawling around the soil near school campus.<\/p>\n",Formicidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_2944-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/arthopodgel2.png,","
For Wolbachia Gel:<\/p>\n
*Wells starting near ruler<\/p>\n
Lane 1: HindIII ladder w\/ UView<\/p>\n
Lane 2: H20 w\/ UView<\/p>\n
Lane 3: Positive DNA control w\/ UView<\/p>\n
Lane 4: Negative Arthropod control w\/ UView<\/p>\n
Lane 5: Positive Arthropod control w\/ UView<\/p>\n
Lane 6: Arthropod #1 (Pogonomyrmex barbatus)<\/p>\n
Lane 7: Arthropod #2 (Monomorium minimum)<\/p>\n
Lane 8: Blank<\/p>\n
For Arthropod Gel:<\/p>\n
*Wells starting near ruler<\/p>\n
Lane 1: Blank<\/p>\n
Lane 2: HindIII ladder w\/ UView<\/p>\n
Lane 3: H20 w\/ UView<\/p>\n
Lane 4: Positive DNA control w\/ UView<\/p>\n
Lane 5: Negative Arthropod control w\/ UView<\/p>\n
Lane 6: Positive Arthropod control w\/ UView<\/p>\n
Lane 7: Arthropod #1 (Pogonomyrmex barbatus)<\/p>\n
Lane 8: Arthropod #2 (Monomorium minimum)<\/p>\n
Setting: 100V constant, 45 minutes<\/p>\n
*Using Separate Power Boxes<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Screenshot-2024-05-23-at-12.24.49-PM.png,",33.8609884000,-118.2542285000,05/14/2024,"
Wild-caught. Caught when surrounded by the same type of arthropods. Is red and orange colored, found near dirt mound around school campus.<\/p>\n",Formicidae,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_2944-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/arthopodgel2.png,","
For Wolbachia Gel:<\/p>\n
*Wells starting near ruler<\/p>\n
Lane 1: HindIII ladder w\/ UView<\/p>\n
Lane 2: H20 w\/ UView<\/p>\n
Lane 3: Positive DNA control w\/ UView<\/p>\n
Lane 4: Negative Arthropod control w\/ UView<\/p>\n
Lane 5: Positive Arthropod control w\/ UView<\/p>\n
Lane 6: Arthropod #1 (Pogonomyrmex barbatus)<\/p>\n
Lane 7: Arthropod #2 (Monomorium minimum)<\/p>\n
Lane 8: Blank<\/p>\n
For Arthropod Gel:<\/p>\n
*Wells starting near ruler<\/p>\n
Lane 1: Blank<\/p>\n
Lane 2: HindIII ladder w\/ UView<\/p>\n
Lane 3: H20 w\/ UView<\/p>\n
Lane 4: Positive DNA control w\/ UView<\/p>\n
Lane 5: Negative Arthropod control w\/ UView<\/p>\n
Lane 6: Positive Arthropod control w\/ UView<\/p>\n
Lane 7: Arthropod #1 (Pogonomyrmex barbatus)<\/p>\n
Lane 8: Arthropod #2 (Monomorium minimum)<\/p>\n
Setting: 100V constant, 45 minutes<\/p>\n
*Using Separate Power Boxes<\/p>\n
<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Cockroach2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Cockroach1.jpg,",33.8609977840,-118.2548346996,05/14/2024,"
Cockroach caught from a tiny hole on CAMS campus in cement. Lured out using puffcorn pieces.<\/p>\n",Ectobiidae,tweezers,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Arthropod-Gel-Group-3.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Wolbachia-Gel-Group-3.jpg,","
Both gels were run for around 30 minutes using orange g loading dye, then Fast Blast with 100x for 3 minutes. Then the gels were rinsed under warm water for 10 seconds and destained with warm water for 5 minutes off the rocker and 5 minutes on a slow rocker.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Silverfish.jpg,",33.8610315606,-118.2548318855,05/14/2024,"
Silverfish was caught coming out of a hole in cement located on the CAMS campus.<\/p>\n",Lepismatidae,tweezers,Abdomen,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Arthropod-Gel-Group-3.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Wolbachia-Gel-Group-3.jpg,","
Both gels were run for around 30 minutes using orange g loading dye, then Fast Blast with 100x for 3 minutes. Then the gels were rinsed under warm water for 10 seconds and destained with warm water for 5 minutes off the rocker and 5 minutes on a slow rocker.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Ant2.jpg,",33.8607815367,-118.2527526830,05/08/2024,"
Ant caught in wild environment with dry terrain and lots of flora.\u00a0 Ant was caught intact with no wings.<\/p>\n",Formicidae,none,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Arthropod-Gel-Group-3.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Wolbachia-Gel-Group-3.jpg,","
Both gels were run for around 30 minutes using orange g loading dye, then Fast Blast with 100x for 3 minutes. Then the gels were rinsed under warm water for 10 seconds and destained with warm water for 5 minutes off the rocker and 5 minutes on a slow rocker.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Ant1.jpg,",33.8608314882,-118.2527674476,05/08/2024,"
Ant found in wild environment with dry terrain and lots of flora.\u00a0 Ant has no wings and remained intact.<\/p>\n",Formicidae,none,body without wings,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Arthropod-Gel-Group-3.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Wolbachia-Gel-Group-3.jpg,","
Both gels were run for around 30 minutes using orange g loading dye, then Fast Blast with 100x for 3 minutes. Then the gels were rinsed under warm water for 10 seconds and destained with warm water for 5 minutes off the rocker and 5 minutes on a slow rocker.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/20240513_093722-scaled.jpg,",33.8810120000,-118.1822336000,05/12/2024,"
$\"\"$ <\/p>\n
The arthropod was found in a grassy farm area with large trees near a small pool of water. It was collected in the morning, around 10 am, springtime, and it was around 71 degrees F outside. The arthropod has large red eyes, a tan abdomen, a black rear, wings, and is small in size. A picture of where the arthropod was collected and the trap around its natural habitat is attached.<\/p>\n",Drosophila melanogaster,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/arthropod-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/wolbachia-1.png,","
DNA extraction:\u00a0<\/strong>The arthropod was crushed really well. There was no need to extract the abdomen out as the arthropod was really small.<\/p>\n
Gel Electrophoresis lanes<\/strong> for CO1 Arthropod PCR:<\/p>\n
$\"\"$ <\/p>\n
1- Well 2:\u00a0DNA Ladder<\/strong><\/p>\n
2- Well 4:\u00a0Common Fruit Fly Sample 4<\/strong><\/p>\n
3- Well 5: Common Fruit Fly Sample 3<\/strong><\/p>\n
4- Well 6: H2O (Water Control)<\/strong><\/p>\n
5- Well 7: Soft-Winged Flower Beetle Sample 2<\/strong><\/p>\n
6- Well 8: Soft-Winged Flower Beetle Sample 1<\/strong><\/p>\n
7- Well 10: Negative (-) Arthropod Control<\/strong><\/p>\n
8- Well 11: Positive (+) Arthropod Control<\/strong><\/p>\n
9- Well 12: Positive (+) DNA Control<\/strong><\/p>\n
Gel Electrophoresis lanes<\/strong> for 16S rRNA Wolbachia PCR:<\/p>\n
$\"\"$ <\/p>\n
1- Well 2:\u00a0DNA Ladder<\/strong><\/p>\n
2- Well 4:\u00a0Common Fruit Fly Sample 4<\/strong><\/p>\n
3- Well 5: Common Fruit Fly Sample 3<\/strong><\/p>\n
4- Well 6: H2O (Water Control)<\/strong><\/p>\n
5- Well 7: Soft-Winged Flower Beetle Sample 1<\/strong><\/p>\n
6- Well 8: Soft-Winged Flower Beetle Sample 2<\/strong><\/p>\n
7- Well 10: Negative (-) Arthropod Control<\/strong><\/p>\n
8- Well 11: Positive (+) Arthropod Control<\/strong><\/p>\n
9- Well 12: Positive (+) DNA Control<\/strong><\/p>\n
Analysis:\u00a0<\/strong>There were bands for Positive (+) Arthropod Control, Negative (-) Arthropod Control, and Positive (+) DNA Control for the CO1 Arthropod PCR Gel. The Negative (-) Arthropod Control did not show any bands in the 16S rRNA Wolbachia PCR Gel and the Water Control did not have bands at all, showing that all the controls worked. This indicates that the results of this experiment are valid and could be analyzed.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/20240513_093819-scaled.jpg,",33.8810120000,-118.1822336000,05/12/2024,"
$\"\"$ <\/p>\n
The arthropod was found in a grassy farm area with large trees near a small pool of water. It was collected in the morning, around 10 am, springtime, and it was around 71 degrees F outside. The arthropod has large red eyes, a tan abdomen, a black rear, wings, and is small in size. A picture of where the arthropod was collected and the trap around its natural habitat is attached.<\/p>\n",Drosophila melanogaster,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/arthropod-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/wolbachia-1.png,","
DNA extraction:\u00a0<\/strong>The arthropod was crushed really well. There was no need to extract the abdomen out as the arthropod was really small.<\/p>\n
Gel Electrophoresis lanes<\/strong> for CO1 Arthropod PCR:<\/p>\n
$\"\"$ <\/p>\n
1- Well 2:\u00a0DNA Ladder<\/strong><\/p>\n
2- Well 4:\u00a0Common Fruit Fly Sample 4<\/strong><\/p>\n
3- Well 5: Common Fruit Fly Sample 3<\/strong><\/p>\n
4- Well 6: H2O (Water Control)<\/strong><\/p>\n
5- Well 7: Soft-Winged Flower Beetle Sample 2<\/strong><\/p>\n
6- Well 8: Soft-Winged Flower Beetle Sample 1<\/strong><\/p>\n
7- Well 10: Negative (-) Arthropod Control<\/strong><\/p>\n
8- Well 11: Positive (+) Arthropod Control<\/strong><\/p>\n
9- Well 12: Positive (+) DNA Control<\/strong><\/p>\n
Gel Electrophoresis lanes<\/strong> for 16S rRNA Wolbachia PCR:<\/p>\n
$\"\"$ <\/p>\n
1- Well 2:\u00a0DNA Ladder<\/strong><\/p>\n
2- Well 4:\u00a0Common Fruit Fly Sample 4<\/strong><\/p>\n
3- Well 5: Common Fruit Fly Sample 3<\/strong><\/p>\n
4- Well 6: H2O (Water Control)<\/strong><\/p>\n
5- Well 7: Soft-Winged Flower Beetle Sample 1<\/strong><\/p>\n
6- Well 8: Soft-Winged Flower Beetle Sample 2<\/strong><\/p>\n
7- Well 10: Negative (-) Arthropod Control<\/strong><\/p>\n
8- Well 11: Positive (+) Arthropod Control<\/strong><\/p>\n
9- Well 12: Positive (+) DNA Control<\/strong><\/p>\n
Analysis:\u00a0<\/strong>There were bands for Positive (+) Arthropod Control, Negative (-) Arthropod Control, and Positive (+) DNA Control for the CO1 Arthropod PCR Gel. The Negative (-) Arthropod Control did not show any bands in the 16S rRNA Wolbachia PCR Gel and the Water Control did not have bands at all, showing that all the controls worked. This indicates that the results of this experiment are valid and could be analyzed.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_1089-2-3-scaled.jpg,",33.8595578000,-118.2539130000,05/12/2024,"
$\"\"$ <\/p>\n
The arthropod was found in a field with large shrubs and native flowers. It was collected in the afternoon, around 2 pm, springtime, and it was around 72 degrees F outside. The arthropod is black, 6-legged, has antennas, small in size, and has a segmented body. A picture is attached of the arthropod in its natural habitat, there were many on the flower, but only 1 was collected.<\/p>\n",Dasytes coerulescens,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/arthropod-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/wolbachia-1.png,","
DNA extraction:\u00a0<\/strong>The arthropod was crushed really well. There was no need to extract the abdomen out as the arthropod was really small.<\/p>\n
Gel Electrophoresis lanes<\/strong> for CO1 Arthropod PCR:<\/p>\n
$\"\"$ <\/p>\n
1- Well 2:\u00a0DNA Ladder<\/strong><\/p>\n
2- Well 4:\u00a0Common Fruit Fly Sample 4<\/strong><\/p>\n
3- Well 5: Common Fruit Fly Sample 3<\/strong><\/p>\n
4- Well 6: H2O (Water Control)<\/strong><\/p>\n
5- Well 7: Soft-Winged Flower Beetle Sample 2<\/strong><\/p>\n
6- Well 8: Soft-Winged Flower Beetle Sample 1<\/strong><\/p>\n
7- Well 10: Negative (-) Arthropod Control<\/strong><\/p>\n
8- Well 11: Positive (+) Arthropod Control<\/strong><\/p>\n
9- Well 12: Positive (+) DNA Control<\/strong><\/p>\n
Gel Electrophoresis lanes<\/strong> for 16S rRNA Wolbachia PCR:<\/p>\n
$\"\"$ <\/p>\n
1- Well 2:\u00a0DNA Ladder<\/strong><\/p>\n
2- Well 4:\u00a0Common Fruit Fly Sample 4<\/strong><\/p>\n
3- Well 5: Common Fruit Fly Sample 3<\/strong><\/p>\n
4- Well 6: H2O (Water Control)<\/strong><\/p>\n
5- Well 7: Soft-Winged Flower Beetle Sample 1<\/strong><\/p>\n
6- Well 8: Soft-Winged Flower Beetle Sample 2<\/strong><\/p>\n
7- Well 10: Negative (-) Arthropod Control<\/strong><\/p>\n
8- Well 11: Positive (+) Arthropod Control<\/strong><\/p>\n
9- Well 12: Positive (+) DNA Control<\/strong><\/p>\n
Analysis:\u00a0<\/strong>There were bands for Positive (+) Arthropod Control, Negative (-) Arthropod Control, and Positive (+) DNA Control for the CO1 Arthropod PCR Gel. The Negative (-) Arthropod Control did not show any bands in the 16S rRNA Wolbachia PCR Gel and the Water Control did not have bands at all, showing that all the controls worked. This indicates that the results of this experiment are valid and could be analyzed.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_1093-2-copy-1-scaled.jpg,",33.8592900000,-118.2551200000,05/12/2024,"
$\"\"$ <\/p>\n
The arthropod was found in a field with large shrubs and native flowers. It was collected in the afternoon, around 1 pm, springtime, and it was around 72 degrees F outside. The arthropod is black, 6-legged, has antennas, small in size, and has a segmented body. A picture is attached of the arthropod in its natural habitat, there were many on the flower, but only 1 was collected.<\/p>\n",Dasytes coerulescens,DNeasy (Qiagen) blood and tissue kit,Whole arthropod,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/arthropod-1.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/wolbachia-1.png,","
DNA extraction:\u00a0<\/strong>The arthropod was crushed really well. There was no need to extract the abdomen out as the arthropod was really small.<\/p>\n
Gel Electrophoresis lanes<\/strong> for CO1 Arthropod PCR:<\/p>\n
$\"\"$ <\/p>\n
1- Well 2:\u00a0DNA Ladder<\/strong><\/p>\n
2- Well 4:\u00a0Common Fruit Fly Sample 4<\/strong><\/p>\n
3- Well 5: Common Fruit Fly Sample 3<\/strong><\/p>\n
4- Well 6: H2O (Water Control)<\/strong><\/p>\n
5- Well 7: Soft-Winged Flower Beetle Sample 2<\/strong><\/p>\n
6- Well 8: Soft-Winged Flower Beetle Sample 1<\/strong><\/p>\n
7- Well 10: Negative (-) Arthropod Control<\/strong><\/p>\n
8- Well 11: Positive (+) Arthropod Control<\/strong><\/p>\n
9- Well 12: Positive (+) DNA Control<\/strong><\/p>\n
Gel Electrophoresis lanes<\/strong> for 16S rRNA Wolbachia PCR:<\/p>\n
$\"\"$ <\/p>\n
1- Well 2:\u00a0DNA Ladder<\/strong><\/p>\n
2- Well 4:\u00a0Common Fruit Fly Sample 4<\/strong><\/p>\n
3- Well 5: Common Fruit Fly Sample 3<\/strong><\/p>\n
4- Well 6: H2O (Water Control)<\/strong><\/p>\n
5- Well 7: Soft-Winged Flower Beetle Sample 1<\/strong><\/p>\n
6- Well 8: Soft-Winged Flower Beetle Sample 2<\/strong><\/p>\n
7- Well 10: Negative (-) Arthropod Control<\/strong><\/p>\n
8- Well 11: Positive (+) Arthropod Control<\/strong><\/p>\n
9- Well 12: Positive (+) DNA Control<\/strong><\/p>\n
Analysis:\u00a0<\/strong>There were bands for Positive (+) Arthropod Control, Negative (-) Arthropod Control, and Positive (+) DNA Control for the CO1 Arthropod PCR Gel. The Negative (-) Arthropod Control did not show any bands in the 16S rRNA Wolbachia PCR Gel and the Water Control did not have bands at all, showing that all the controls worked. This indicates that the results of this experiment are valid and could be analyzed.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_7392-1-scaled.jpg,",33.8610274938,-118.2557101919,05/13/2024,"
Caught in sparse, dry, and flat environments. The bug was crawling on a flower prior to capture. Temperature was roughly 64\u00ba-68\u00baF. The surrounding plants were dry bushes or short and stubby trees. Weeds also present.<\/p>\n",Coccinellidae,tweezers,body without wings,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/91CCDF32-E148-4C60-AC73-83C676C47830.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_1770.jpg,",33.8172755446,-118.2819989324,05/12/2024,"
About 4mm x\u00a0 3.25mm x 2.75mm, black\/grey, 14 legs, has minimal spots on lower half of exoskeleton<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Gonads dissected,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Untitled_Artwork-1.jpg,","
Run gel at 125V for 30 minutes using DNA control ladder with UView.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_1763.jpg,",33.8172755440,-118.2819989000,05/12/2024,"
About 3.5mm x\u00a0 2.75mm x 2.5mm, black\/grey with green spots across the entire exoskeleton, 14 legs<\/p>\n",Arthropoda,DNeasy (Qiagen) blood and tissue kit,Gonads dissected,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Untitled_Artwork.jpg,","
Run gel at 125V for 30 minutes using DNA control ladder with UView.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_2322-1.jpeg,",33.8594990995,-118.2547079436,05/15/2024,"
Found in a mass field mixed with a lot of plants.<\/p>\n",Anthomyiidae,pcr,Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Wolbachia-Postive.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/CO1.jpg,","",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/KZU_048_Protapion-Fulvipes.jpg,",47.5183182999,8.5493758466,08/25/2023,"",Coleoptera,Instagene Matrix,Abdomen,MiniOne,TBE,Midori 1:20'000,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/KZU-048_gel-Alena-Jorina.jpg,","
despite the faint band we received decent sequences<\/p>\n",No,TATTGAATTAGGTAACCCTGGATCATTAATTGGTAATGATCAAATTTATAATGTTATTGTCACAGCTCATGCATTTATTATAATTTTTTTTATAGTTATACCTGTTATAATTGGAGGATTTGGTAATTGATTAATTCCTTTAATATTAGGAGCACCTGATATGGCATTTCCTCGAATAAATAATATAAGATTTTGACTTTTACCTCCTTCTCTAACTCTTTTATTAATAAGAAGAATTATTGAAAAAGGCGCAGGAACCGGATGAACTGTTTACCCTCCTTTAGCTGCTAATATTGCTCACAGAGGAGCTTCTGTTGATTTAGCAATTTTTAGTCTCCATTTAGCTGGAATCTCATCAATTTTAGGTGCTGTAAATTTTATTTCTACTATTATAAATATACNACCTACTGGAATATCTCTTGATCAACTATCACTTTTCACTTGAGCTGTAAAAATTACAGCTATTCTACTTTTATTATCTTNACCTGTTTTANCAGGGGC,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/048_Blatt_Sequenz_F-1.fasta,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/nnhzqx4z.png,",33.78500862172772,-118.26984503333826,05/10/2024,"
The moth was caught outside at night on top of a leaf in a garden.<\/p>\n",Geometridae,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Wolbachia.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Arthropod.png,","
Wolbachia Gel Lanes (Left)<\/strong>
\nWells 1-2: Empty
\nWell 3: DNA Ladder
\nWell 4: Arthropod (Winged) #1: Fruit Fly
\nWell 5: Arthropod (Winged) #2: Housefly
\nWell 6: Arthropod (Winged) #3: Pale Beauty Moth
\nWell 7: Positive Arthropod Control
\nWell 8: Negative Arthropod Control
\nWell 9: Positive DNA Control
\nWell 10: Water Control
\nWells 11-15: Empty<\/p>\n
Arthropod Gel Lanes (Right)<\/strong>
\nWells 1-2: Empty
\nWell 3: DNA Ladder
\nWell 4: Arthropod (Winged) #1: Fruit Fly
\nWell 5: Arthropod (Winged) #2: Housefly
\nWell 6: Arthropod (Winged) #3: Pale Beauty Moth
\nWell 7: Positive Arthropod Control
\nWell 8: Negative Arthropod Control
\nWell 9: Positive DNA Control
\nWell 10: Water Control
\nWells 11-15: Empty<\/p>\n
We diluted 100X Fast Blast to 1X and left the gels to stain overnight on a rocker.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/phncvcg9.png,",33.786063387005356,-118.26912491351837,05/10/2024,"
The housefly was caught outside on the rims of a trashcan.<\/p>\n",Diptera,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Wolbachia.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Arthropod.png,","
Wolbachia Gel Lanes (Left)<\/strong>
\nWells 1-2: Empty
\nWell 3: DNA Ladder
\nWell 4: Arthropod (Winged) #1 – Fruit Fly
\nWell 5: Arthropod (Winged) #2 – Housefly
\nWell 6: Arthropod (Winged) #3 – Pale Beauty Moth
\nWell 7: Positive Arthropod Control
\nWell 8: Negative Arthropod Control
\nWell 9: Positive DNA Control
\nWell 10: Water Control
\nWells 11-15: Empty<\/p>\n
Arthropod Gel Lanes (Right)<\/strong>
\nWells 1-2: Empty
\nWell 3: DNA Ladder
\nWell 4: Arthropod (Winged) #1 – Fruit Fly
\nWell 5: Arthropod (Winged) #2 – Housefly
\nWell 6: Arthropod (Winged) #3 – Pale Beauty Moth
\nWell 7: Positive Arthropod Control
\nWell 8: Negative Arthropod Control
\nWell 9: Positive DNA Control
\nWell 10: Water Control
\nWells 11-15: Empty<\/p>\n
We diluted 100x Fast Blast to 1X and left the gels to stain overnight on a rocker.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/cwj81u4s.png,",33.7844080916,-118.2597197214,05/10/2024,"
The fruit fly was caught on top of a few fruits inside a kitchen.<\/p>\n",Diptera,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,Fast Blast,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Arthropod.png,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Wolbachia.png,","
Wolbachia Gel Lanes (Right)<\/strong>
\nWells 1-2: Empty
\nWell 3: DNA Ladder
\nWell 4: Arthropod (Winged) #1 – Fruit Fly
\nWell 5: Arthropod (Winged) #2 – Housefly
\nWell 6: Arthropod (Winged) #3 – Pale Beauty Moth
\nWell 7: Positive Arthropod Control
\nWell 8: Negative Arthropod Control
\nWell 9: Positive DNA Control
\nWell 10: Water Control
\nWells 11-15: Empty<\/p>\n
Arthropod Gel Lanes (Left)<\/strong>
\nWells 1-2: Empty
\nWell 3: DNA Ladder
\nWell 4: Arthropod (Winged) #1 – Fruit Fly
\nWell 5: Arthropod (Winged) #2 – Housefly
\nWell 6: Arthropod (Winged) #3 – Pale Beauty Moth
\nWell 7: Positive Arthropod Control
\nWell 8: Negative Arthropod Control
\nWell 9: Positive DNA Control
\nWell 10: Water Control
\nWells 11-15: Empty<\/p>\n
We diluted 100x Fast Blast to 1X and left the gels to stain overnight on a rocker.<\/p>\n",Unknown,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/IMG_8316-scaled-e1716483768357.jpeg,",33.9127111,-118.2308706,04/27/2024,"
This jumping spider was caught as it crawled along a wall inside a household.<\/p>\n",Arachnida,tweezers,Abdomen,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/image000000-e1716789620259.jpeg,","
Ran gel for 60 minutes at 115 V and 60amps and visualized under UV light.<\/p>\n",Yes,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/D_Wingless2.png,",33.8316745000,-118.2816930000,05/10/2024,"
The arthropod was observed crawling on the ground when cause. It is roughly 2-4 mm in length, black and brown in color, and has two antennae and six legs. Collection took place around 9:00 am, in 64 degree Fahrenheit weather with overcast skies. The arthropod was collected on a school campus by a pile of leaves. Other arthropods were present in the pile of leaves, but were not collected.<\/p>\n",Linepithema humile,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Annotated-Gels-1-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Annotated-Gels-6.jpg,","
Wolbachia\u00a0<\/strong><\/em>Gel Electrophoresis Lanes:<\/strong><\/p>\n
Lanes 1-3 – Empty<\/p>\n
Lane 4 – DNA ladder<\/p>\n
Lane 5 – Winged arthropod 1 (Typical mosquito)<\/p>\n
Lane 6 – Winged arthropod 2 (F-winged barklouse)<\/p>\n
Lane 7 – Wingless arthropod 1 (Praying mantis)<\/p>\n
Lane 8 – Wingless arthropod 2 (Argentine ant)<\/p>\n
Lane 9 – (+) Arthropod control<\/p>\n
Lane 10 – (-) Arthropod control<\/p>\n
Lane 11 – (+) DNA control<\/p>\n
Lane 12 – Water control<\/p>\n
Wolbachia\u00a0<\/strong><\/em>Gel Electrophoresis Analysis:<\/strong><\/p>\n
Both the arthropod and DNA controls worked, meaning DNA extraction, Wolbachia<\/em> PCR, and the Wolbachia<\/em> gel electrophoresis were successful. Lane 8, containing the Argentine ant DNA sample did not show any bands, meaning Wolbachia\u00a0<\/em>was not present in the arthropod.<\/p>\n
Arthropod Gel Electrophoresis Lanes:<\/strong><\/p>\n
Lanes 1-3 – Empty<\/p>\n
Lane 4 – DNA ladder<\/p>\n
Lane 5 – Winged arthropod 1 (Typical mosquito)<\/p>\n
Lane 6 – Winged arthropod 2 (F-winged barklouse)<\/p>\n
Lane 7 – Wingless arthropod 1 (Praying mantis)<\/p>\n
Lane 8 – Wingless arthropod 2 (Argentine ant)<\/p>\n
Lane 9 – (+) Arthropod control<\/p>\n
Lane 10 – (-) Arthropod control<\/p>\n
Lane 11 – (+) DNA control<\/p>\n
Lane 12 – Water control<\/p>\n
Arthropod Gel Electrophoresis Analysis:<\/strong><\/p>\n
Only the DNA ladder showed banding. None of the samples or the positive controls showed bands, meaning an error was made in the procedure. The\u00a0Wolbachia\u00a0<\/em>yielded expected control results, so DNA extraction and\u00a0Wolbachia\u00a0<\/em>PCR were likely successful. An error was mostly likely made during arthropod PCR, since the arthropod gel electrophoresis was successful (since the DNA ladder was correctly visualized).<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/C_Wingless1.png,",33.8316745000,-118.2816930000,05/10/2024,"
The arthropod has six legs and two antennae. The arthropod is also multicolored and was observed crawling. The end of its abdomen is curled. It was collected around 9:00 am in 64 degree Fahrenheit weather, with overcast skies. The arthropod was found near a pile of leaves by a gutter. Other arthropods were present in the leaves but not collected.<\/p>\n",Stagmomantis,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Annotated-Gels-2.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Annotated-Gels-5.jpg,","
Wolbachia\u00a0<\/strong><\/em>Gel Electrophoresis Lanes:<\/strong><\/p>\n
Lanes 1-3 – Empty<\/p>\n
Lane 4 – DNA ladder<\/p>\n
Lane 5 – Winged arthropod 1 (Typical mosquito)<\/p>\n
Lane 6 – Winged arthropod 2 (F-winged barklouse)<\/p>\n
Lane 7 – Wingless arthropod 1 (Praying mantis)<\/p>\n
Lane 8 – Wingless arthropod 2 (Argentine ant)<\/p>\n
Lane 9 – (+) Arthropod control<\/p>\n
Lane 10 – (-) Arthropod control<\/p>\n
Lane 11 – (+) DNA control<\/p>\n
Lane 12 – Water control<\/p>\n
Wolbachia\u00a0<\/strong><\/em>Gel Electrophoresis Analysis:<\/strong><\/p>\n
Both the arthropod and DNA controls worked, meaning DNA extraction, Wolbachia<\/em> PCR, and the Wolbachia<\/em> gel electrophoresis were successful. Lane 7, containing the Praying mantis DNA sample did not show any bands, meaning Wolbachia\u00a0<\/em>was not present in the arthropod.<\/p>\n
Arthropod Gel Electrophoresis Lanes:<\/strong><\/p>\n
Lanes 1-3 – Empty<\/p>\n
Lane 4 – DNA ladder<\/p>\n
Lane 5 – Winged arthropod 1 (Typical mosquito)<\/p>\n
Lane 6 – Winged arthropod 2 (F-winged barklouse)<\/p>\n
Lane 7 – Wingless arthropod 1 (Praying mantis)<\/p>\n
Lane 8 – Wingless arthropod 2 (Argentine ant)<\/p>\n
Lane 9 – (+) Arthropod control<\/p>\n
Lane 10 – (-) Arthropod control<\/p>\n
Lane 11 – (+) DNA control<\/p>\n
Lane 12 – Water control<\/p>\n
Arthropod Gel Electrophoresis Analysis:<\/strong><\/p>\n
The only banding visible was from the DNA ladder. None of the samples or controls showed any banding, meaning an error was made in the procedure. Because the\u00a0Wolbachia\u00a0<\/em>gel electrophoresis was successful and the controls yielded expected results, DNA extraction and Wolbachia\u00a0<\/em>PCR were successful, meaning an error was likely made during arthropod PCR.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/B_Winged2.png,",33.8316745000,-118.2816930000,05/10/2024,"
The arthropod was found on the bark of a tree trunk and jumped into collection petri dish. It was collected around 9:00 am, with overcast skies and a temperature of about 64 degrees Fahrenheit. The tree was located on a school campus by a few other trees and a grassy area.<\/p>\n",Graphopsocus cruciatus,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Annotated-Gels-1.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Annotated-Gels-4.jpg,","
Wolbachia\u00a0<\/strong><\/em>Gel Electrophoresis Lanes:<\/strong><\/p>\n
Lanes 1-3 – Empty<\/p>\n
Lane 4 – DNA ladder<\/p>\n
Lane 5 – Winged arthropod 1 (Typical mosquito)<\/p>\n
Lane 6 – Winged arthropod 2 (F-winged barklouse)<\/p>\n
Lane 7 – Wingless arthropod 1 (Praying mantis)<\/p>\n
Lane 8 – Wingless arthropod 2 (Argentine ant)<\/p>\n
Lane 9 – (+) Arthropod control<\/p>\n
Lane 10 – (-) Arthropod control<\/p>\n
Lane 11 – (+) DNA control<\/p>\n
Lane 12 – Water control<\/p>\n
Wolbachia\u00a0<\/strong><\/em>Gel Electrophoresis Analysis:<\/strong><\/p>\n
Both the arthropod and DNA controls worked, meaning DNA extraction, Wolbachia<\/em> PCR, and the Wolbachia<\/em> gel electrophoresis were successful. Lane 6, containing the F-winged barklouse DNA sample did not show any bands, meaning Wolbachia\u00a0<\/em>was not present in the arthropod.<\/p>\n
Arthropod Gel Electrophoresis Lanes:<\/strong><\/p>\n
Lanes 1-3 – Empty<\/p>\n
Lane 4 – DNA ladder<\/p>\n
Lane 5 – Winged arthropod 1 (Typical mosquito)<\/p>\n
Lane 6 – Winged arthropod 2 (F-winged barklouse)<\/p>\n
Lane 7 – Wingless arthropod 1 (Praying mantis)<\/p>\n
Lane 8 – Wingless arthropod 2 (Argentine ant)<\/p>\n
Lane 9 – (+) Arthropod control<\/p>\n
Lane 10 – (-) Arthropod control<\/p>\n
Lane 11 – (+) DNA control<\/p>\n
Lane 12 – Water control<\/p>\n
Arthropod Gel Electrophoresis Analysis:<\/strong><\/p>\n
Other than the DNA ladder, no banding was present in the arthropod gel. Since the positive arthropod and DNA controls did not yield expected results, an error was likely made somewhere in the procedure. The\u00a0Wolbachia\u00a0<\/em>controls worked as expected, so DNA extraction and\u00a0Wolbachia\u00a0<\/em>were likely successful. An error likely occurred during arthropod PCR, since gel electrophoresis was successful (DNA ladder bands appeared).<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/A_Winged1.png,",33.8316745000,-118.2816930000,05/10/2024,"
This arthropod was collected by a school building, near a tree. The surrounding area was grassy and shady, with some leaves on the ground. The arthropod was seen crawling on the tree trunk around 9:00 am. It was overcast and 64 degrees Fahrenheit outside. Some ants were also seen on the tree trunk.<\/p>\n",Culex,DNeasy (Qiagen),Whole arthropod,Standard electrophoresis system,TAE,UView,"https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Annotated-Gels.jpg,https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/Annotated-Gels-3.jpg,","
Wolbachia<\/em> Gel Electrophoresis Lanes:<\/strong><\/p>\n
Lanes 1-3 – Empty<\/p>\n
Lane 4 – DNA ladder<\/p>\n
Lane 5 – Winged arthropod 1 (Typical mosquito)<\/p>\n
Lane 6 – Winged arthropod 2 (F-winged barklouse)<\/p>\n
Lane 7 – Wingless arthropod 1 (Praying mantis)<\/p>\n
Lane 8 – Wingless arthropod 2 (Argentine ant)<\/p>\n
Lane 9 – Positive arthropod control<\/p>\n
Lane 10 – Negative arthropod control<\/p>\n
Lane 11 – Positive DNA control<\/p>\n
Lane 12 – Negative DNA control (sterile water)<\/p>\n
Lanes 13-15 – Empty<\/p>\n
Wolbachia<\/em> Gel Electrophoresis Analysis:<\/strong><\/p>\n
Both the arthropod and DNA controls worked, meaning DNA extraction, Wolbachia<\/em> PCR, and the Wolbachia<\/em> gel electrophoresis were successful. Lane 5, containing the typical mosquito DNA, did not show any bands, meaning Wolbachia<\/em> was not present in the arthropod.<\/p>\n
Arthropod\u00a0Gel Electrophoresis Lanes:<\/strong><\/p>\n
Lanes 1-3 – Empty<\/p>\n
Lane 4 – DNA ladder<\/p>\n
Lane 5 – Winged arthropod 1 (Typical mosquito)<\/p>\n
Lane 6 – Winged arthropod 2 (F-winged barklouse)<\/p>\n
Lane 7 – Wingless arthropod 1 (Praying mantis)<\/p>\n
Lane 8 – Wingless arthropod 2 (Argentine ant)<\/p>\n
Lane 9 – Positive arthropod control<\/p>\n
Lane 10 – Negative arthropod control<\/p>\n
Lane 11 – Positive DNA control<\/p>\n
Lane 12 – Negative DNA control (sterile water)<\/p>\n
Lanes 13-15 – Empty<\/p>\n
Arthropod Gel Electrophoresis Analysis:<\/strong><\/p>\n
Besides the DNA ladder, no banding was present, including in the controls. Since the positive arthropod and positive DNA control worked in the\u00a0Wolbachia\u00a0<\/em>gel electrophoresis, DNA extraction and\u00a0Wolbachia\u00a0<\/em>PCR were likely successful. Errors were likely made during arthropod PCR since the DNA ladder was successfully visualized, indicating that gel electrophoresis was successful.<\/p>\n",No,,,, "https://wolbachiaprojectdb.org/wp-content/uploads/2024/05/S2.jpg,",33.862178612,-118.2547159,05/13/2024,"
Approx 4.5mm x 2mm x 2.5mm, exoskeleton consisting of overlapping blue-gray shells with white-green longitudinal spot pattern<\/p>\n