Pill Bug

October 2, 2023

Sample information
Picture
Photos by: Violet P.
Location
Collection date 08/29/2023
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations
  • Curled up when touched as a response to environmental stimuli
  • Hard outer shell
  • Many legs
  • Size of pill
Putative identification Arthropoda Malacostraca Isopoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Results did not show arthropod-positive DNA, therefore, it is not likely that this test was accurate enough to be sure that the arthropod did not have Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be negative for Wolbachia.
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