| Metadata Field | Description |
| Picture | Image of the arthropod. .jpg, .jpeg, .png, .gif file formats are accepted up to 24MB. |
| Photo Credit | First name and last initial. Multiple names should be separated by commas. |
| Location | City or general area of collection. Do not include home address. |
| Collection date | Date the arthropod was collected. |
| Captive/Cultivated? | Captive/cultivated arthropods refer to those purchased from a store, lab-reared, or donated by an insectary. Wild-caught arthropods are those collected from the natural environment. |
| Group | This is assigned based on your school or research group. |
| Observations | Environmental observations – describe the habitat, distinguishing features, weather, arthropod behavior, etc. |
| Putative Identification | Identify at least to order, if possible. Select the most resolved classification group possible. The identification can be modified after DNA sequencing. |
| Arthropod not listed? | If the taxonomic group is not listed above, enter here so that it may be added to the database. |
| Extraction kit | DNA extraction method |
| DNA extraction location | Arthropod DNA source (whole, abdomen, larval, other) |
| Gel image | Image of agarose gel (following gel electrophoresis) with properly labeled ladder, sample, and controls, and sample lane outlined. .jpg, .jpeg, .png, .gif file formats are accepted up to 24MB. |
| Gel electrophoresis system | Type of gel electrophoresis system |
| Buffer | Gel electrophoresis running buffer |
| DNA stain | Stain used to visualize DNA in gel electrophoresis |
| Protocol notes | Additional details about the experimental method. Were additional steps added? Did anything unexpected happen during the experiment? Were errors made? If unable to label the gel image, identify each lane here. |
| Wolbachia positive | Is the arthropod infected with Wolbachia? |
| Confidence level | Were you able to confidently detect Wolbachia based on quality and success of the controls? Rate your confidence level. |
| Explain your confidence level | Describe results in terms of experimental controls. Did all of the controls work? Did the experiment go as planned? |
| Wolbachia 16S .fasta | Modified Wolbachia sequence file, after quality control (see Lab 5) |
| Wolbachia 16S .ab1 | Original Wolbachia sequencing chromatogram file (raw data) |
| Wolbachia 16S sequence | Wolbachia sequence, text version of the .fasta sequence |
| Arthropod COI .fasta | Modified arthropod sequence file, after quality control (see Lab 5) |
| Arthropod COI .ab1 | Original arthropod sequencing chromatogram file (raw data) |
| Arthropod COI sequence | Arthropod sequence, text version of the .fasta sequence |
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus
Fly- Pollen pediculata
Cricket- Acheta domesticus