BROaccMJB FALL 2023

November 6, 2023

Sample information
Picture
Photos by: Mikaela J. Bennett
Location
Collection date
Captive / Cultivated?
Group Georgia Southern University
Observations

I observed we gotten them from the garden bed in the GSU Armstrong campus
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

I felt that I could have done better to get the insect gene as well.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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