BROACCF23-1 Hemiptera

November 6, 2023

Sample information
Picture
Photos by: Halie B
Location
Collection date 08/21/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • 8/21/2023​
  • ~1:40 pm​
  • Student gardens near Science Center, GSU Armstrong Campus​
  • Garden beds and surrounding vegetation were beaten with a stick, insects fell onto a sheet and were collected with forceps​​
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Positive, Negative, and No Template Controls were as expected. Sample ID shortened to HRB: Insect CO1 gene was detected, but not Wolbachia 16s gene. Results are conclusive.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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