MAC.ACI.F23-22_coleoptera

November 8, 2023

Sample information
Picture
Photos by: Isabella T.
Location
Collection date 08/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collection Method: Glove and Pan

Collection Habitat: Tall Grass
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The DNA was very clean
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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