BROACH23TSV Orthoptera

November 8, 2023

Sample information
Picture
Photos by: Thai V.
Location
Collection date 08/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Sweep net collection method.​
  • 1:30-2:00 P.M. | 8/23/23.​
  • Fields on Arts drive at GSU Armstrong campus.​
  • Sweep net across tall grass.​
  • Pinch bottom of net — >put in jar –> let bugs go in jar.2
Putative identification Arthropoda Hexapoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Positive control, negative control, and NTC results are as expected.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be postive for Wolbachia.
Report Inappropriate Post