Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/28/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | Next to a path in a mixed forest, half-shade |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae Aedes Aedes cinereus |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | CO – 708 bp –> a visible line 16S rRNA – 438 bp –> a visible line |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
ATAGTAGGAACATCTTTAAGTATATTAATTCGTGCAGAATTAAGTCATCCAAGTATATTTATTGGAAATGACCAAATTTATAATGTAATTGTAACAGCTCATGCATTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTTGGTAATTGACTTGTTCCTTTAATATTAGGAGCCCCTGATATAGCCTTTCCTCGAATAAATAATATAAGTTTTTGAATATTACCTCCCTCATTAACCCTGTTATTATCAAGCTCAATAGTAGAAAATGGAGCTGGTACAGGGTGAACTGTTTACCCTCCTTTATCTTCTGGAACTGCTCATGCAGGAGGTTCTGTTGACTTAGCAATTTTCTCTTTACATTTAGCCGGGATTTCTTCGATTTTAGGGGCCGTAAATTTTATTACAACTGTTATTAATATGCGATCAGCAGGAATTACACTTGATCGACTTCCTTTATTTGTTTGATCAGTTGTTATTACTGCTATTTTATTACTTCTTTCTTTACCTGTTTTAGCTGGGGCTATTACTATATTATTAACTGATCGAAATTTAAATACATCA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Aedes cinereus was found to be postive for Wolbachia. |
ant
Ochlerotatus Trivittatus
Aedes, Ochlerotatus trivittatus
Ochlerotatus Trivittatus
Ochlerotatus trivittatus