Sample information |
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| Picture |
|
|---|---|
| Location | |
| Collection date | 08/28/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | Approx. 1.50 metres above the forest floor in an area where the sun’s rays reach well. |
| Putative identification | Arthropoda Insecta Hemiptera |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | The gel image shows that the sample is Wolbachia positive. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
CTTGKAACCCATTAATTAATAATGATCAAATTTATAATTCTCTTGTAACTAGCCATGCCWWTATTATAATTTTTTTTATAGTTATACCATTTATAATTGGGGGCTTTGGTAATTTTTTAATCCCTTTAATATTAGGGTCACCTGATATAGCATACCCMCGAATAAATAACATAAGATTTTGATTACTTCCCCCTTCAATTATACTATTAATATTAAGAAATTTTTTAAGGAATGGAGKAGGKRCRGGATGAACCATTTACCCCCCTCYAGCTTCTAATATWTTCCWTAGAGGACCTTCAATTGATATATSARKCKTTTCTCTTCATATCGCTGGAATATCAKCT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence | Download FASTA
Download AB1
TTAGGATCTTGTAACCCATTAATTAATAATGATCAAATTTATAATTCTCTTGTAACTAGCCATGCCTTTATTATAATTTTTTTTATAGTTATACCATTTATAATTGGGGGCTTTGGTAATTTTTTAATCCCTTTAATATTAGGGTCACCTGATATAGCATACCCACGAATAAATAACATAAGATTTTGATTACTTCCCCCTTCAATTATACTATTAATATTAAGAAATTTTTTAAGGAATGGAGTAGGTACAGGATGAACCATTTACCCCCCTCTAGCTTCTAATATTTTCCATAGAGG
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Hemiptera was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid