Firebug

Sample information

Picture
Photos by: Elin H.
Location
Collection date 08/20/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

The bug was found near a flowerbed on a hot stone with in it‘s colony.

Putative identification Arthropoda Hexapoda Insecta Hemiptera Pyrrhocoridae Pyrrhocoris

Methods

Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer
DNA stain Midori 1:20'000
Gel images
Protocol notes

This bug didn‘t show any results on both PCRs. This might be due to an error in the DNA extraction or the PCR process. There is a possibility that some chemicals or bad conditions lead to the DNA degrading.

Results

Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

There were no results in both PCRs. The second PCR was even done by the teacher.

Wolbachia 16S sequence
Arthropod COI sequence
Summary
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