Otiorynchus sulcatus

October 22, 2024

Sample information
Picture
Photos by: Luna.S, Tharanya.S
Location No map location data available.
Collection date 08/20/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

Caught in my garden
Putative identification Arthropoda

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer
DNA stain Midori 1:20'000
Gel images
Protocol notes

Something went wrong during the DNA extraction because there was no sign of a line.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

We are struggling to see our mistake during the whole process of preparing the PCR because every step was made by the same person and in the same way and in other cases there were clear results. Furthermore ovaries with eggs were found here which should have given us clear results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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