Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/19/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Palm Beach Atlantic University |
| Observations | |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | pcr |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | Gel electrophoresis only occurred for 15 minutes due to time constraints |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | Ant 1 is thought to be positive due to a faint fragment 600BP in size while the positive Wolbachia control and Wolbachia DNA have a fragment 560BP in size. Due to the short time of electrophoresis, it is difficult to confirm if this fragment is a similar size as the positive control and Wolbachia DNA. Ant 2 had no banding associated with Wolbachia and thus was negative. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid