Two black Ants

October 22, 2024

Sample information
Picture
Photos by: Noah P
Location
Collection date 09/19/2024
Captive / Cultivated? Wild-caught
Group Palm Beach Atlantic University
Observations
Putative identification Arthropoda

Methods
Extraction kit pcr
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

Gel electrophoresis only occurred for 15 minutes due to time constraints

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Ant 1 is thought to be positive due to a faint fragment 600BP in size while the positive Wolbachia control and Wolbachia DNA have a fragment 560BP in size. Due to the short time of electrophoresis, it is difficult to confirm if this fragment is a similar size as the  positive control and Wolbachia DNA. Ant 2 had no banding associated with Wolbachia and thus was negative.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be postive for Wolbachia.
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