Two black Ants

Sample information
Picture	Photos by: Noah P
Location
Collection date	09/19/2024
Captive / Cultivated?	Wild-caught
Group	Palm Beach Atlantic University
Observations
Putative identification	Arthropoda
Methods
Extraction kit	pcr
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Other
Gel images
Protocol notes	Gel electrophoresis only occurred for 15 minutes due to time constraints
Results
Wolbachia presence	Yes
Confidence level	Medium
Explanation of confidence level	Ant 1 is thought to be positive due to a faint fragment 600BP in size while the positive Wolbachia control and Wolbachia DNA have a fragment 560BP in size. Due to the short time of electrophoresis, it is difficult to confirm if this fragment is a similar size as the positive control and Wolbachia DNA. Ant 2 had no banding associated with Wolbachia and thus was negative.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Arthropoda was found to be postive for Wolbachia.

The Wolbachia Project Database