Pill Bug – Parasteatoda tepidariorum

October 24, 2024

Sample information
Picture
Photos by: Dr. Kerry O’Brien
Location
Collection date 09/04/2024
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations

Under a couple of leaves of a bush, on top of a wooden plank, summer, 75°, terrestrial habitat
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of our controls seemed to appear just fine on the gel and we did not have any unexpected difficulties with our electrophoresis process. There is great variety in our gel, and my DNA simply doesn’t have any indication of Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
GCTTGGGCTGCAATAGTGGGAACAGCAATAAGAGTANATTCGAATTGAGTTAGGTCAAACAGGTAGATTTATGGGTGATGATCAATTATATAATGTAATTGTAACGGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATACCAATTTTAATTGGAGGGTTTGGAAATTGATTAGTTCCATTAATATTAGGGGCACCTGACATAGCATTTCCTCGAATAAATAATTTGAGATTTTGATTATTACCCCCTTCTTTATTATTATTATTTATTTCGTCTATAGTAGAAATAGGAGTAGGAGCTGGGTGAACTGTATATCCACCTCTATCATCTTTAGATGGTCACGGTGGTAGATCAGTAGATTTTGCAATTTTTTCTTTGCATTTAGCTGGGGCTTCTTCTATTATAGGTGCGGTAAATTTTATTTCTACTGTACTCAATATGCGATCTTATGGGATGAGAATAGAAAAAGTTCCTTTATTTGTATGATCAGTTCTAATTACTGCTGTGCTATTATTATTATCATTACCAGTATTAGCAGGTGCTATTACTATATTATTAACTGATCGAAATTTTAATACTTCATTTTTTGATCCTGCAGGAGGAGGTGATCCTATTTTGTTTCAGCATTTATTCTGATTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Armadillidium was found to be negative for Wolbachia.
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