Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/25/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Kantonsschule Zuercher Unterland |
| Observations | Captured: In the garden at home in Oberweningen Condition: Alive when caught and then frozen to death and then preserved in alcohol |
| Putative identification | Arthropoda Hexapoda Insecta Diptera |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | Midori 1:20'000 |
| Gel images |
|
| Protocol notes | In both PCR processes meaningful/good results could be observed. We refer to the results in lane 6 (picture 1) and lane 2 (picture 2). |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | There is no mark on the gel. The first try of the PCR process, done by us students, failed but the second one, done by our teacher, showed clear results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Diptera was found to be negative for Wolbachia. |
Spider TBD