Sample information |
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Picture |
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Location | |
Collection date | 11/06/2024 |
Captive / Cultivated? | Wild-caught |
Group | Georgia Southern University |
Observations | Used a sweeping net to collect the insec and collected around 2:00pm in a grassy area. |
Putative identification | Arthropoda Hexapoda Insecta Diptera |
Methods |
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Extraction kit | |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | Other |
Gel images |
|
Protocol notes | DNA Extraction kit of in-house reagents was used. |
Results |
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Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | The DNA extraction, PCR, and Gel Electrophoresis processes went smoothly. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary | The Diptera was found to be negative for Wolbachia. |