Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | This ant was found crawling on a tree. Under the tree there appeared to be a nest covered in leaves where other ants walked out of. |
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Lane 11 contains my sample for this arthropod. |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | On our wolbachia gel there was no bar at the 438 mark, indicating that there was no Wolbachia DNA in my sample. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
NNNNTNNNNNNNGNCNTCTGANCTGGATAATTGGATCTTCNATAAGAATACTTATTCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTAATTCTATATTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCCTTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATCAGTTTACTTTTATTAAGAAATTTCATTAATGACGGAGTGGGAACAGGATGAACTGTTTACCCTCCATTAGCTTCTAATATTTTTCATAATGGTCCTTCAGTTGACCTGACAATTTTTTCTCTACATATTGCAGGAATATCTTCTATTCTAGGAGCTATCAATTTTATTTCAACAATTCTAAATATACGACATAAAAATTTTTCTTCTGATAAAATCCCTTTACTTGTTTGATCTATTTTAATTACAGCAATTTTACTTCTTCTATCTTTACCAGTTCTAGCTGGAGCTATTACTATACTTTTAACTGACCGGAACCTTAATACTTCCTTTTTTGACCCCTCAGGAGGAGGAGACCCTGTTCTTTTATCAACATTTATTCTGATTTTTTGGTCACCCTGAANTTNAAAA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Formicidae was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid