Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | Highly active and mobile; surrounded by other species of millipede; flat segments of nearly uniform brown plates (with some flecks of darker brown and white) are overlain to create level appearance; legs are translucent in appearance |
| Putative identification | Arthropoda Diplopoda Polydesmida Polydesmidae Polydesmus Polydesmus angustus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Body without legs |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | I did not have quality levels over 30 in order to trust my data. Our controls did not yield valid results. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
|
| Arthropod COI sequence |
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid