Portland State University – Araneus diadematus

November 25, 2024

Sample information
Picture
Location
Collection date 10/15/2024
Captive / Cultivated? Wild-caught
Group Portland State University
Observations
Putative identification Arthropoda Chelicerata Arachnida Araneae Araneidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Gel A, Wells 17-19. Well 17 is positive for arthropod DNA, well 18 is positive for Wolbachia positive control, well 19 is negative for Wolbachia in the spider
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Araneidae was found to be negative for Wolbachia.
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