PECKS SKIPPER FOR AYESHA MALIK

November 26, 2024

Sample information
Picture
Photos by: AYESHA MALIK
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

IT WAS A DRY WARM DAY. WE COLLECTED BY THE POND NEAR BENEDICTINE UNIVERSITY PARKING GARAGE
Putative identification Arthropoda Hexapoda Insecta Lepidoptera

Methods
Extraction kit Qiagen Blood and Tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer Other
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

WOLBACHIA BAND WAS NOT FOUND ON MY GEL ELECTROPHORESIS. HOWEVER, ACCORDING TO DATA OVER THE INTERNET IT DIDNT STATE THAT MY INSECT DOES HAVE WOLBACHIA.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
>47-A_AG1_Co1R (1).scf (380 bp) AAAACAGGTAAAGATAAGAGTAACAATAAAGCTGTAATTCCTACAGATCAAACAAATAAAGGTATTTGGTCAAATGATAA ATTTTTAATTCGTATATTAATGATTGTTGTAATAAAATTAATAGCTCCTAAAATTGAAGAGATTCCAGCTAAATGAAGAG AAAAAATTGCTAAATCAACAGAAGATCCTTGATGAGCAATATTAGAAGATAAAGGGGGGTAAACTGTTCAACCTGTTCCT GCACCATTTTCTACAATTCTTCTTGAAATTAATAATATTAATGAAGGAGGTAATATTCAAAAACTTATATTATTTATTCG AGGGAAAGCTATATCAGGAGCTCCTAGTATTAAAGGTACTAATCAATTTCCAAATCCTCC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lepidoptera was found to be negative for Wolbachia.
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