December 4, 2024

Sample information
Picture
Photos by: Kacper P.
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

collected by Fall 2024 students, I collected this specimen with a jar-and-lid method on a milkweed plant by a pond.
Putative identification Arthropoda Hexapoda Insecta Hemiptera Lygaeidae Oncopeltus Oncopeltus fasciatus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was absolutely no indication of Wolbachia in my insect
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Oncopeltus fasciatus was found to be negative for Wolbachia.
Report Inappropriate Post