Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 02/15/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Berkshire Community College |
| Observations | Flying through living room in winter. |
| Putative identification | Arthropoda Insecta Diptera Culicidae Anopheles Anopheles punctipennis |
Methods |
|
| Extraction kit | Monarch DNA extraction (NEB) |
| DNA extraction location | body without wings |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | TAQ polymerase: NE Bio Labs ONETAQ Hot Start Quick-Load 2x MM w/ Standard Buffer (product #M0488S). DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product #N0559S) Annealing temperature at 49C. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The controls worked as expected, and the lines are very defined at the fragment levels. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Anopheles punctipennis was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid