Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 02/28/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Singapore American School |
| Observations | Small, black, dead beetle with visible head. |
| Putative identification | Arthropoda Insecta Coleoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The controls worked and gave expected results, so the procedure was likely correct. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Coleoptera was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid