Beetle

March 14, 2025

Sample information
Picture
Location
Collection date 02/28/2025
Captive / Cultivated? Wild-caught
Group Singapore American School
Observations

Small, black, dead beetle with visible head.
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Other
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls worked and gave expected results, so the procedure was likely correct.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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