Arthropod 2 (grain moth)

March 26, 2025

Sample information
Picture
Photos by: Alyssa S., Ava S., Yamil N.
Location
Collection date 01/02/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations
Putative identification Arthropoda Insecta Lepidoptera Gelechiidae

Methods
Extraction kit Blood and Tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Lane

1- DNA ladder

2- Arthropod one (Ghost spider)

3- Arthropod two (grain moth)

4- Drosophila Positive

5- Drosophila Negative

6- DNA control

7- water

8 Blank

9- DNA ladder

10- Arthropod one (Ghost Spider)

11- Arthropod two (Grain moth)

12- Wolbachia positive control

13- Wolbachia Negative control

14- DNA control

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Not an expert. First extraction.
Wolbachia 16S sequence
Arthropod COI sequence
NNNNNNNNNNNNNNNGCAGGTATAGTAGGAACATCTTTAAGATTATTAATTCGTGCTGAATTAGGAAATCCTGGATCATT AATTGGTGATGATCAAATTTATAATACTATTGTAACAGCTCATGCTTTCATTATAATTTTTTTTATAGTTATACCTATTA TAATTGGAGGATTTGGAAATTGATTAGTTCCTCTTATATTAGGAGCTCCAGATATAGCTTTCCCTCGAATAAACAATATA AGATTTTGACTCCTTCCCCCTTCTATTATACTTTTAATTTCAAGTAGAATTGTAGAAAATGGAGCAGGTACAGGATGAAC AGTTTACCCCCCACTTTCATCTAATATTGCTCATAGAGGAAGATCAGTAGATTTAGCAATCTTTTCTCTTCATTTAGCCG GAATTTCATCAATTTTAGGAGCTGTAAATTTTATTACAACTATTATTAATATACGCCCTAATAATATATCCTTAGATCAA ATACCCCTATTTGTTTGATCTGTAGGAATTACAGCTTTATTATTACTTTTATCTCTACCTGTATTAGCAGGTGCAATTAC CATATTATTAACAGATCGAAATTTAAATACATCATTTTTTGATCCTGCAGGAGGAGGTGACCCCATTCTTTATCAACATT TATTTTGATTTTTTNGGTCACCCTGAANTTTAANN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Gelechiidae was found to be negative for Wolbachia.
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