False Honey Ant

June 4, 2025

Sample information
Picture
Photos by: Esme Shea
Location
Collection date 03/31/2025
Captive / Cultivated? Wild-caught
Group Scientists in Residence
Observations

I found the bug near a bush on a dirty brick pathway, climbing around on the ground, it was sunny, and the bug was by itself.
Putative identification Arthropoda Insecta Hymenoptera Formicidae Prenolepis Prenolepis imparis

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

When doing the Wolbachia Gel there was a hole poked into the gel lane 3.

My Arthropod was in lane 3 of both gels.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls worked as they were meant to.
Wolbachia 16S sequence
CTTCAATAAGAATACTTATCCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTAATTCTATA GTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCC TTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATCAGTT TACTTTTATTAAGAAATTTCATTAATGACGGAGTGGGGACAGGATGAACTGTTTACCCTCCATTAGCTTCTAATATTTTT CATAATGGTCCTTCAGTTGACCTAACAATTTTTTCTCTGCATATTGCAGGGATATCCTCTATTCTAGGAGCTATCAATTT TATTTCAACAATTTTAAATATACGACATAAAAATTTTTCTTCTGATAAAATCCCTTTACTTGTTTGATCTATTTTAATTA CAGCAATTTTACTTCTTCTTTCTTTACCAGTTCTAGCTGGAGCTATTACTATACTTTTAACTGACCGAAACCTTAATACT TCCTTTTTTGATCCCTCAGGAGGGGG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
CTTCAATAAGAATACTTATCCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTAATTCTATA GTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCC TTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATCAGTT TACTTTTATTAAGAAATTTCATTAATGACGGAGTGGGGACAGGATGAACTGTTTACCCTCCATTAGCTTCTAATATTTTT CATAATGGTCCTTCAGTTGACCTAACAATTTTTTCTCTGCATATTGCAGGGATATCCTCTATTCTAGGAGCTATCAATTT TATTTCAACAATTTTAAATATACGACATAAAAATTTTTCTTCTGATAAAATCCCTTTACTTGTTTGATCTATTTTAATTA CAGCAATTTTACTTCTTCTTTCTTTACCAGTTCTAGCTGGAGCTATTACTATACTTTTAACTGACCGAAACCTTAATACT TCCTTTTTTGATCCCTCAGGAGGGGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Prenolepis imparis was found to be negative for Wolbachia.
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