Brown Marmorated Stink Bug

April 18, 2025

Sample information
Picture
Photos by: Gaby Meredith
Location
Collection date 03/31/2025
Captive / Cultivated? Wild-caught
Group Scientists in Residence
Observations

This bug was found on a curtain for a glass door near the kitchen. It had been sunny outside and the bug was taken in the evening. The bug was alone and had been flying around the chandelier that was turned on.
Putative identification Arthropoda Insecta Hemiptera Pentatomidae Halyomorpha Halyomorpha halys

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

My arthropod was in well two in Gel 1. All of Gel 2 contained positive controls besides well seven. In Gel 1, wells one, four, and six were also positive while the others were negative.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of the controls worked as they were supposed to.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TNCAGCTATAAGATTAATTATCCGTATCGAATTAGGACAGCCTGGAAGATTTATTGGTAATGATCAAATTTATAATGTAA TTGTAACAGCACATGCATTTGTAATAATTTTCTTTATAGTAATACCAATCATAATTGGAGGATTCGGTAATTGATTAGTC CCTTTAATAATTGGAGCACCTGATATAGCCTTCCCACGATTAAATAATATAAGATTCTGATTATTACCCCCTTCATTAAC TTTATTATTAATAAGAAGAATAGCAGAATCAGGAGCTGGGACAGGATGAACAGTCTATCCCCCCTTATCAAGTAATATCT CACATAGAGGAGCATCAGTTGATTTAGCTATTTTTTCCTTACATTTGGCTGGGGTTTCATCAATTTTAGGGGCAGTAAAT TTTATTTCAACTATTATAAATATACGACCAACAGGTATAACCCCTGAACGAATCCCATTGTTTGTGTGATCAGTAGGAAT TACTGCACTTCTTCTGTTATTATCCTTACCTGTGTTAGCAGGTGCTATTACTATACTATTAACTGACCGAAATTTTAATA CCTCTTTTTTTGACCCATCAGGAGGA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Halyomorpha halys was found to be negative for Wolbachia.
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