Chauliognathus pensylvanicus

April 15, 2025

Sample information
Picture
Photos by: Alexis S.
Location
Collection date 09/03/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2024 students
Putative identification Arthropoda Insecta Coleoptera Cantharidae Chauliognathus Chauliognathus pensylvanicus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

11-Electrophoresis

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

There was no band from my gel electrophoresis.
Wolbachia 16S sequence Download FASTA    Download AB1
>W1R.ab1 (357 bp) CCGTGGCGTGCTGATCCACGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGG CTTTTAAGGGATTAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTCCATA AGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCT GATGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGC AACACCTGTGTGAAACCCGGCCGAACCGACCCTATCC
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA   
>A1.ab1 (688 bp) ATATTGTTGGGGTATAAATTGGGTCTCCCCCACCGGTTGGGTCAAAAAAAGAAGAATTTAAGTTTCGGTCAGTTAATAGT ATAGTAATTGCTCCTGCAAGAACTGGTAGTGATAGTAATAAAAGAATTGCTGTAATAAGAACAGATCATACAAATAAAGG AGTTTTATCAAGTATTATACCAAATGGACGTATATTTATAATAGTTGAAATGAAATTTACTGCACCTAAAATTGAGGAAA TTCCTGCTAAATGTAAACTAAAAATCACTAAATCTACTGAAGGCCCATTATGTGTTAAATTAGAAGAAAGAGGAGGGTAA ACAGTTCATCCTGTTCCTGCCCCTATTTCTACGATTGTTCTTAAAATTAAAAGAGTTAAAGCAGGGGGTAAAAGTCAAAA TCTTATGTTATTTAATCGTGGAAATGCTATATCAGGAGCTCCAATTATTAAAGGAACTAATCAATTTCCAAAACCCCCAA TTATAATAGGTATTACTATAAAGAAAATTATAATGAAAGCATGAGCTGTAACAATTATATTATAAATTTGGTCGTTTCCA ATTAATCTTCCTCTAGTTCCTAATTCTAACCGAATTAAAATACTTAACGATGTTCCTACTATTCCTGCCCATATTCCAAA TAAAAAGTATAATGTTCCAATATCATTATGTTTGTTTGAAAACCAAAG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Chauliognathus pensylvanicus was found to be negative for Wolbachia.
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