ZET-BDB Lepidoptera

April 15, 2025

Sample information
Picture
Photos by: Benjamin B.
Location
Collection date 01/28/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Found on the base of a tree. Collected into a jar using hands.
Putative identification Arthropoda Insecta Lepidoptera

Methods
Extraction kit
DNA extraction location head and lower body
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was no 16S rRNA band in the gel and it did not show under sequencing.
Wolbachia 16S sequence
Arthropod COI sequence
GTTGGAACATCTTTAAGAATTTTAATTCGAGCTGAATTAGGACATCCTGG AGCATTAATTGGAGATGATCAAATTTATAATGTAATTGTAACTGCACATG CTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGTGGATTT GGAAATTGATTAGTGCCTTTAATATTAGGTGCTCCTGATATAGCATTCCC ACGAATAAATAATATAAGATTTTGACTTCTACCTCCTGCTCTTTCTTTAC TATTAGTAAGTAGAATAGTTGAAAATGGAGCTGGGACAGGATGAACTGTT TATCCACCTCTATCCGCTGGAATTGCTCATGGTGGAGCTTCAGTTGATTT AGCTATTTTTTCTCTACATTTAGCAGGAATTTCTTCAATTTTAGGAGCTG TAAATTTTATTACAACTGTAATTAATATACGATCAACAGGAATTTCATTA GATCGTATACCTTTATTTGTTTGATCAGTAGTTATTACTGCTTTATTATT ATTATTATCACTTCCAGTACTAGCAGGAGCTATTACTATATTATTAACAG ATCGAAATTTAAATACATCATTTTTTGACCCAGCGGGAGGAGGAGATCCT ATTTTATACCAACATTTATTTTGATTTTTTGGTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lepidoptera was found to be negative for Wolbachia.
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