April 15, 2025

Sample information
Picture
Photos by: Yusra B
Location
Collection date 11/02/2030
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

collected by Fall 2024 students
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

It was above 99%
Wolbachia 16S sequence
>w8R.ab1 (353 bp) CACCGTGGCGTGTGCCACGATTACTAGCGATTCCACTTCATGCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGGCT TTTAAGGGATTAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTCCATAAG GGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCTGA TGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAA CACCTGTGTGAAACCCGGCCGAACCGACCCTAT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
66-A_AP2_Co1_R (1).ab1 (598 bp) TGTATTTAAATTTCGATCAGTTAAAAGTATATGTAATAGCTCCTGCTAACACAGGAAGAGAAAGAAGAAGTAATACTGCA GTAATTAAAACAGCTCAAACAAATAAAGGTATTTGGTCTATTTTTATTTCTGAAGATCGTATATTTATAATTGTTGAAAT GAAATTTACAGCACCTAGAATTGATGAAATCCCTGCTATATGAAGTCTAAAAATAGATAAATCTACTGAAGATCCTCTAT GAGCAATATTAGAAGATAAAGGAGGGTATACTGTTCACCCAGTTCCAGTTCCATTTTCTACAATTCTTCTTATTAGTAAT AGAGATAATGATGGGGGTAAAAATCAGAATCTTATATTATTTATTCGTGGGAATGCTATATCAGGTGCTCCTAATATTAG TGGAACTAATCAATTTCCAAATCCACCAATTATGATTGGCATAACTATAAAGAAAATTATAATAAAAGCGTGTGCAGTTA CAACAACATTGTAAATTTGATCATTCCCAATTAGAGCATTTGTATTTCCTAATTCTGCTCGAATTAAAAGTCTTAGGGAT GTTCCTAGTATTCCTGACCATGCTCCAAATAAAAAATA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be negative for Wolbachia.
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