Eupeodes sp

April 17, 2025

Sample information
Picture
Photos by: Zaid S
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

The sample was collected by Fall 2024 students.
Putative identification Arthropoda Insecta Diptera Syrphidae Eupeodes

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The sample resulted in a thick Wolbachia gel band and a 100% ID match in BLAST.
Wolbachia 16S sequence Download FASTA    Download AB1
>A_WB2_Wo_R .scf (312 bp) TGTACTCGAGTTGCAGAGTACAATCCGAACTGAGATGTCTTTTAGGGATTAGCTTAGGCTTGCGCACCTTGCAGCCCATT GTAGACACCATTGTAGCACGTGTGTAGCCCACTTCATAAAGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGCT TATCACTGGCAGTTTCCTTAAAGTACTCAGCATTACCTGATAGCAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTA ACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACTTGTGTAAAATCCGGCCGAACCGACCCTA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
>A_AB2_Co1_R AATGATAAAAGTAATAATAAAGCAGTAATTACTACTGATCATACAAATAAAGGTATACGATCATAAGAAATTCCATTAGA TCGTATATTAATTACTGTAGTAATAAAATTTACAGCTCCTAAAATTGAAGATATACCAGCTAGATGAAGAGAAAAAATAG CTAAATCAACANAANCTCNNCCATGTGCAATACTAGCAGAAAGTGGAGGGTAAACTGTTCAACCAGTTCCAGCTCCATTT TCAACTATTCTTCTTACTAATAAAAGAGTTAAAGATGGGGGTAATAATCAAAAACTTATATTATTTATACGAGGAAAAGC TATATCAGGAGCTCCAAGTATTAAAGGAACTAATCAATTTCCAAATCCTCCAATTATAATAGGTATAACTATAAAAAAAA TTATAACAAAAGCATGAGCAGTTACAATTACATTATAAATTTGATCATCACCAATTAAAGCTCCTGGATGACCAAGTTCT GCACGAATTAATACACTTAAAGAAGTACCTACTATACCTGCTCAAGTACCAAATAAAAAATATAATG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Eupeodes was found to be postive for Wolbachia.
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