Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/30/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | |
| Putative identification | Arthropoda Insecta Diptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | The result of Wolbachia was very clear using Gel Electrophoresis. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
GTAGGAACATCTCTAAGAATTTTAATTCGAGCTGAATTAGGGCACCCAGG AGCTTTAATTGGAGATGATCAAATTTATAACGTAATTGTTACTGCACATG CTTTTGTAATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGATTT GGAAATTGACTAGTCCCATTAATATTAGGAGCACCTGATATAGCATTTCC TCGAATAAATAATATAAGTTTTTGATTACTTCCACCATCATTAACACTTT TAATGGCTAGAAGTATAGTGGAAAATGGAGCCGGAACAGGATGAACTGTA TATCCACCTTTATCTTCAATTATTGCACATGGGGGAGCTTCAGTAGATTT AGCTATTTTTTCTCTTCATTTAGCTGGGGTATCATCAATTTTAGGAGCAG TAAATTTTATTACTACAGTAATTAATATACGATCTACAGGAATTACATTC GATCGAATACCATTATTTGTATGATCAGTAGTAATTACTGCTTTATTATT ACTATTATCACTACCAGTATTAGCAGGAGCCATTACTATATTATTAACTG ATCGAAATTTAAATACTTCATTCTTTGATCCTGCAGGAGGAGGGGATCCA ATTTTATATCAACATTTATTTTGATTTTTTG
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Diptera was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid