GRE-ASB – Hemiptera

April 17, 2025

Sample information
Picture
Photos by: Azaria B.
Location No map location data available.
Collection date 01/30/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

I collected my insect at Georgia Southern University, near the pond behind the Science Building. My collection site was an area of dead grass near the pond, and I used a sweep net to capture my insect.
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I was very confident in my gel electrophoresis results, which indicated that my insect was “not positive” for Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
ATAATTGgatAATATTAAGAATAATTATTCGAATTGAACTAGcactttca gCATCATTATTCTTTTTAGATAACAGCCACATTTATAACGTAATCGTTAC CTCTCACGCATTTATTATAATCTTCTTTATAGTCATACCAATTATAATTG GGGGATTTGGAAACTGACTTATCCCAATTATAATCGGGGCACCTGACATA GCATTTCCACGTCTAAATAATATAAGATTTTGACTTTTGCCACCATCTCT TACTTTACTACTAATAAGATCTATAATTGAAATAGGGGTAGGAACAGGCT GAACCGTATACCCACCATTATCAGGAAATATTACACATGCAGGGCCTAGT GTAGATATATCAATTTTCTCTCTACATTTAGCAGGAATCTCGTCAATTTT AGGTTCGATTAATTTTATCACTACAATCTTTAATATACGAACAGAAGGCA TAACAATACACCTTACACCGCTATTTGTATGATCTGTATTAATTACAGCC ATTCTTCTATTATTATCACTTCCAGTTCTTGCAGGAGCAATTACTATACT ATTAACAGACCGAAACCTGAATACAACATTCTTTGACCCATCGGGAGGGG GAGATCCAATTTTATACCAGCATTTATTTTGATTTTTTGGTC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hemiptera was found to be negative for Wolbachia.
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