Uroleucon leonardi

April 25, 2025

Sample information
Picture
Photos by: Aaliya I.
Location
Collection date 09/03/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2024 students
Putative identification Arthropoda Insecta Hemiptera Aphididae Uroleucon Uroleucon leonardi

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Controls were successful. My insect did not have Wolbachia but had DNA.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Did not have a BLAST hit because my arthropod did not have a Wolbachia band present so there was no usable band present, but I did have a prominent usable band of DNA with a BLAST match of 99.63% hence the medium confidence. I know that I can trust my results because of the consistent BLAST match results all being from the same Taxonomic Order and family.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
AATGATAAAATTAATAAAATANCNGTAATTAAAATTGATCAAGGGAAAAGAGGAATTTGATTTAATTTTATATTATTTGG TATTATATTTATAATTGTACAAATAAAATTAATTGCTCCTAAAATTGATGAAATTCCTGCTAAATGTAAAGAGAAAATTG TTAAATCAACTGAAATATTATTATGTGCAATATTATTTGATAATGGTGGATAAATAGTTCATCCTGTTCCTGTTCCATTA TTAATTATAAAACTACAAATTATTATTATTAATGAAGGAGGTAATAATCAAAATCTAATATTATTTAAACGTGGAAATGA TATATCAGGACATCCTATTATTATAGGAATTAGTCAATTTCCAAAACCACCAATTACAATTGGTATAGTTATAAAAAAAA TTATAATAAAAGCATGAATTGTTACAATAACATTATATAATTGATTATTATTAATAATTGAATTAATTTGTCTTAATTCT AAACGAATTAAAATTCTAAGTGAAGATCCAATTATTCCTGATCAAATACCAAACAAAAAGTATAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Uroleucon leonardi was found to be negative for Wolbachia.
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