Sample information
Picture	Photos by: Jeremy H
Location
Collection date	05/09/2025
Captive / Cultivated?	Wild-caught
Group	Unionville High School
Observations
Putative identification	Arthropoda Insecta Coleoptera Coccinellidae
Methods
Extraction kit	tweezers
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniOne
Buffer	TBE
DNA stain
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level	Test results showed bars at desired locations for Wolbachia presence.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Coccinellidae was found to be postive for Wolbachia.