Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/07/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | College of Eastern Idaho |
| Observations | Sample code: LW16 |
| Putative identification | Arthropoda Insecta Coleoptera Coccinellidae Adalia Adalia frigida |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | 1X TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
TGAGCAGGAATAGTAGGAACTTCTCTAAGAATTATTATTCGATTAGAATTAGGTACAACTAACAGACTAATTGGAAATGACCAAATTTATAATGTTATTGTAACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCCATTATAATTGGAGGTTTTGGAAACTGACTAGTACCTTTAATAATTGGAGCGCCTGATATGGCATTCCCACGTCTTAATAATATAAGATTTTGATTATTACCTCCAGCTTTAACCCTTTTAATTTCTAGAAGAGTAATTGAAATAGGAGCAGGTACAGGATGAACAGTATATCCACCTCTTTCTTCAAATATAGCACATAATGGACCTTCTGTAGATTTAGTAATTTTTAGATTACACTTAGCTGGAATTTCATCAATTTTAGGAGCTGTAAATTTTATTTCTACTATTATAAATATACGACCTAATGGGATAAATCTAGATAAAACACCTTTATTTGTTTGATCAGTTTTAATTACAGCTATTCTACTACTTTTATCATTACCAGTTCTTGCAGGAGCAATTACTATATTATTAACAGATCGTAATATTAATACATCATTTTTTGACCCTACTGGAGGAGGGGATCCAGTTTTATACCAACACTTATTTTGATTTTT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Adalia frigida was found to be postive for Wolbachia. |
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