Armadillidium vulgare Group 4

May 25, 2025

Sample information
Picture
Photos by: Lucy P.
Location
Collection date 04/28/2025
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TAE
DNA stain UView
Gel images
Protocol notes
lane 2: mm ruler
lane 3: uni. ruler
lane 5: long leg spider
lane 6: roly poly
lane 7: dragon fly
lane 8: black aphids
lane 9: (+) arthro control
lane 10: (-) arthro control
lane 11: (+) DNA control
lane 12: water
lane 14: mm ruler

lane 15: uni ruler

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

There were no bands shown in lane 6 and the DNA controls had bands confirming that there was no wolbachia in the roly poly sample however because our other wolbachia positive samples had a low CRL, we cannot be completely confident in the data results
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be negative for Wolbachia.
Report Inappropriate Post