Anisoptera GROUP 4

May 25, 2025

Sample information
Picture
Photos by: Kimi P.
Location
Collection date 04/30/2025
Captive / Cultivated? Captive / Cultivated
Group California Academy of Mathematics and Science
Observations

dragonfly was collected at California Academy of Mathematics and Science. Weather between 55-65 Fahrenheit.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer
DNA stain UView
Gel images
Protocol notes

lane 2: mm ruler

lane 3: uni. ruler

lane 5: long leg spider

lane 6: roly poly

lane 7: dragon fly

lane 8: black aphids

lane 9: (+) arthro control

lane 10: (-) arthro control

lane 11: (+) DNA control

lane 12: water

lane 14: mm ruler

lane 15: uni. ruler

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

did not send for sequencing
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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