NV 58 Cobweb Spider

April 14, 2021

Sample information
Picture
Photos by: Julia L.
Location
Collection date 10/04/2021
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations
  • It was collected in the morning & the outside temperature was 60 degrees Fahrenheit
  • Spider was located in a cobweb in the top corner of the garage 
  • Spider is smaller than a grain of rice
  • Light brown in color
  • Legs are not long, look proportional to body 
  • Slightly bulbous abdomen 
Putative identification Arthropoda Chelicerata Arachnida Araneae Theridiidae

Methods
Extraction kit Puregene (Qiagen)
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer LB
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Theridiidae was found to be negative for Wolbachia.
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