European Paper Wasp – Polistes Dominula

September 11, 2025

Sample information
Picture
Photos by: 02NHDM01
Location
Collection date 09/11/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Found at a port-a potty. Seemed aggressive when caught. Summoned other yellow jackets
Putative identification Arthropoda Insecta Hymenoptera Vespidae Polistinae Polistes

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am very confident that the presence of Wolbachia does exist in this specimen as the PCR had a band of Wolbachia
Wolbachia 16S sequence
AGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCG CAACCCTCATCCTTAGTTGCCATCAGGTAATGCTGAGTACTTTAAGGAAACTGCCAGTGATAAGCTGGAGGAAGGTGGGG ATGATGTCAAGTCATCATGGCCTTTATGGAGTGGGCTACACACGTGCTACAATGGTGTCTACAATGGGTTGCAAGGTGCG CAAGCCTAAGCTAATCCCTAAAAGACATCTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAGTTGGAATCGCTAG TAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
ACTCTTTANANNNNNANTCGAAAGAATTAGGAGCGNNNCTTCTTTCTNN ATAANGATCATGTTATGATTGAATTAGTACNGCCCCTGCCTTAGGAATAATTTTTTTAATAGGTATNCCNTTTATAATTG GAGGTTTTTGAAAANGATNANTNCACTTAATACTCNAGNNCNCNGAAAAGGCTNTNCCTCTAGNANTNNTNNNANANTTT GAGTTGNACCACCCTCTTTATTTTTGCCTATTTTACAAAAGATAATANAAATAANATCAAGAACAGGAATAACTTGATAC CCCCCCTTGNNTACNNATCTTGGCNATAGTTCGCCTTCCNNNAANNNCGTTTTTGCTCTTCNTTTTGTTGNGNCTCATCC NTATANGACCTATTAAGNNATGGNNCACTATTTNATATACATATGATNACCGAATCATTAAATTTTTNANCTNTATATAC GTGAGCTTTTTTTTATACANCAAAGTTACTTTTATCATATTTACATTTNNTANCGCGAGCGATANCTATANNATTNNNTC TGANAGTANTANATACAANTTTTTTNATCCTAAGGAGAAGGNGAAACANATNNTTTNNAAAANATNTTGGTTTTTTGGCC ACATNNNNNTAAN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Polistes was found to be postive for Wolbachia.
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