Armadillidum nasatum Pillbug

September 11, 2025

Sample information
Picture
Photos by: Heidi M
Location
Collection date 09/11/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Found in a wooded, shaded area, after uncovering a rock, it started to scurry away from the area, and dig deeper into the soil underneath. Once picked, I observed the hard multiple piece shell it had, and many short legs underneath. It was a dark grey in color.
Putative identification Arthropoda Malacostraca Isopoda Armadillidae Armadillidium Armadillidium nasatum

Methods
Extraction kit qiagen blood and tissue
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain Cyber Stain
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My controls showed as they should, with Lane 1 being the DNA Ladder, Lane 2 being my arthropod, Lane 4 being the positive arthropod control, lane 5 as the negative arthropod control, lane 6 as the positive DNA control and 7 being water. This has given me the confidence in knowing if my arthropod is negative for wolbachia.
Wolbachia 16S sequence
TANAGTTATTCGCTAGAACCCTCCCNA NNTNNANCNTCATCCNAAANNGTCATCATGGNNACGGACGGNTCCTGCANTGCNANNGGANTAGTCGTNNNNTNNNNTTC CTGATTAGANNNGGTNNNTTNGTTAANNGNCTTGTNCCTCTTGGNNNGNCNANNTNNTGTNGCTTTCNACNANTANNCAA TCTGGATTTNGACTNTNGNNNGATTTNTTACATTATNAATNGCAATATCANNANNNTCNTCAANANTCGGGGCNGGNTGA NCTGTGCCANTCCNCTCTCGNNNCTGAACNNCTGNNNGNNCANCATNNNNANTGNCCTTNTTCTNNTNCCACTNNTANGT ATTAGATCNNTTATANGAGCTATTANNTTTATTTCTATTATTATNNATTNGNAACCAAGGTNNNNNNNTATATNNAATCC CCTTATTTTGTTTATTANNACCAGTTACACTANTCCATTTTTATTTTCTTTACTANNCTANCCGNANNATTNNNNNNTCT GTNANTANCTAAATCTNNNTNNTTANTTTTTGATCCATCAAGAGNNNCNTNCTACACNGNTCAATTTTATTNNCATATNN TANNAANNAAAAANTTNTANNTATTATTTNTTTATAANNTATNAGNNNNNAATNNNTCATNANTTNNACTTTTTNNTTTN NGTNGTN
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
TTTGGGCAGGGGCTGTAGGTACCGCTTTAAGAATAATTATTCGGACTGAATTAGGGCAGCCTGGGAG TCTCATTGGGGATGATCAAATCTATAATGTAATTGTCACGGCTCATGCTTTTGTAATGATTTTCTTCATAGTAATACCTA TTATAATTGGAGGTTTTGGAAATTGATTAATTCCTTTGATATTAGGGGCTCCTGATATAGCTTTCCCTCGTATAAACAAT ATAAGATTTTGGCTGCTTCCCCCTTCTTTAACTTTACTCCTTAGCAGAGGATTAATTGAAAGAGGAGTAGGAACGGGATG AACTGTTTACCCTCCTTTGGCATCAAGAATTGCCCATAGTGGAGCTTCTGTAGATTTAGGTATCTTTTCTCTTCATCTTG CTGGGGCTTCATCAATTTTAGGGGCAGTAAACTTTATTACGACTATAATTAATATACGGGCGTCCGGAATTAGTATGGAT CGTGTGCCTTTATTTGTTTGGTCTGTTTTAGTAACCGCGGTTCTTCTTCTCTTATCTTTACCTGTTCTTGCTGGAGCTAT TACTATACTATTGACTGACCGTAATTTAAATACATCTTTTTTTGATCCGAGGGGAGGGGGGGATCCTATTCTTTATCAGC ATTTATTCTGATTTTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Armadillidium nasatum was found to be negative for Wolbachia.
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