Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/18/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | We found our sample in a forest (marked on map) beneath the bark of a dead tree. |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae Amaurobiidae Amaurobius Amaurobius fenestralis |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Due to the results of the electrophoresis. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Amaurobius fenestralis was found to be negative for Wolbachia. |
Water Boatman