Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/05/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Galileo Academy of Science and Technology |
| Observations | |
| Putative identification | Arthropoda Insecta Diptera Culicidae |
Methods |
|
| Extraction kit | BABEC |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | The positive control was not seen on the gel, but my DNA sample had visible results, leading to slight uncertainty in the results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Culicidae was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid