Wolbachia(+) Anthrenus sp.

October 24, 2025

Sample information
Picture
Photos by: Elijah M.
Location
Collection date 06/09/2025
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Caught live near yard.
Putative identification Arthropoda Insecta Coleoptera Dermestidae Anthrenus

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results are fourth row. Top: CO1. Bottom: Wol.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Tests worked fine.
Wolbachia 16S sequence
GTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTC
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
GGAACTTCNTTANGAGTATTAGTCCGGACAGAATTAGGTAATCCAGGATCCCTTATTGGAGATGACCAAACATTCAGTGTAATCGTAACAGCCCATGCATTCATTATAATTTTTTTTATAGTTATACCAATTATAATTGGTGGATTCGGTAACTGATTAGTACCCTTAATATTAGGTGCTCCAGATATAGCATTTCCACGAATAAATAATATAAGATTCTGACTTCTACCCCCATCACTATCATTGTTATTAATAAGAAGAATAGTAGAAAAAGGGGCAGGAACAGGATGGACAGTTTACCCCCCACTATCAAGAAATATCGCCCACGGAGGAGCATCAGTAGACCTAGCAATCTTTAGATTACATCTAGCAGGAATCTCATCAATTCTAGGAGCTATTAACTTCATCACCACAGCTATCAATATACGAACTATAGGAATAACTCCAGAACGAATACCCCTATTTGTCTGATCAGTAGCAATTACTGCCCTTCTCTTACTACTCTCATTACCAGTACTAGCAGGAGCTATTACAATACTATTAACAGACCGAAATCTCAATACATCATTCTTTGATCCATCAGGTGGGGGAGACCCAATCCTATACCAACATCTATTCTGATTTTTTGGTCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Anthrenus was found to be postive for Wolbachia.
Report Inappropriate Post