Wolbachia(+) Camponotus pennsylvanicus

November 7, 2025

Sample information
Picture
Photos by: Elijah M.
Location
Collection date 06/21/2025
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Collected live from city park.
Putative identification Arthropoda Insecta Hymenoptera Formicidae Camponotus Camponotus pennsylvanicus

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results are twelfth row. Top: CO1. Bottom: Wol.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Tests worked fine. BLAST results matched.
Wolbachia 16S sequence
GCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTATAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTCACTCGAAGCTA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
GGGATAATTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTAGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTAATTCCACTTATACTAGGATCTCCTGATATAGCTTACCCTCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGAACTATCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTAGTATGATCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGCGCTATTACAATACTACTAACAGACCGAAATCTTAATACTTCATTTTTCGATCCCTCGGGAGGAGGAGATCCTATTTTATACCAACATTTATTTTGATT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Camponotus pennsylvanicus was found to be postive for Wolbachia.
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