GRE-ABS, Hymenoptera

November 11, 2025

Sample information
Picture
Photos by: Aniston S
Location
Collection date 08/26/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

In the soil under pine straw.
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident because of my results, and the methods I used.
Wolbachia 16S sequence
Arthropod COI sequence
TAATTGGATCTTCTTTAAGAATAATTATTCGACTTGAACTTGGGACCTGt tatTCTATTATCAACAATGATCAAATTTTTAATTCTTTAGTTACAGCTCA TGCATTTATTATAATTTTTTTTATAGTTATACCATTTATAATTGGAGGAT TTGGTAATTTTCTTGTCCCTTTAATATTAGGTTCCCCAGATATAGCATAC CCCCGTATAAATAATATAAGATTTTGACTCCTGCCTCCATCTATTTCTCT CCTTCTCTTAAGAAATTTCATTAATAATGGATCTGGGACAGGATGAACTG TTTATCCCCCCTTAGCTTCTAATATTTTTCATAATGGTCCATCAATTGAT TTAACAATTTTTTCTTTACATATTGCAGGAATATCTTCAATTTTAGGAGC AATTAATTTTATTTCTACAATTATTAATATACATCATAAAAATTCATCAA TAGATAAAATTCCCCTTTTAGTTTGATCAATTCTAATTACTGCTATTCTT CTTCTCCTTTCTCTCCCAGTTTTAGCTGGAGCAATCACTATACTTTTAAC TGATCGAAACCTAAATACTTCATTTTTTGATCCTTCTGGAGGAGGAGATC CAATTCTTTATCAACATTTATTTTGATTTTTTGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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