JOEAJD Hemiptera

November 11, 2025

Sample information
Picture
Photos by: Austin D.
Location
Collection date 08/28/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Caught using drag net.
Putative identification Arthropoda Insecta Hemiptera Pentatomidae

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

When extracting, the quality of DNA was extremely high as well as having lots of it. (2.1 quality whereas 1.7 is considered good quality)
Wolbachia 16S sequence
Arthropod COI sequence
CGGATATGAGCAGGTATAGTAGGATCCGCTATAAGACTAATTATCCGAAT CGAACTAGGACAACccggtagatTCATTGGGGATGATCAAATTTATAACG TAATCGTAACagctCACGCCTTCGTAATAATTTTCTTCATAGTAATACCT ATTATAATTGGAGGATTTGGAAACTGACTAGTTCCCCTAATAATCGGAGC TCCCGACATAGCATTCCCTCGTATAAATAACATAAGATTTTGATTATTAC CTCCTTCATTAACATTATTAATAATAAGTAGACTAGCAGAATCAGGAGCT GGAACCGGATGAACAGTTTACCCTCCTTTATCAAGTAATTTATCTCACAG AGGAGCATCCGTAGACTTAGCAATTTTTTCACTCCACTTAGCCGGGGTAT CTTCAATCTTGGGAGCAGTAAATTTTATTTCCACAATCATTAATATACGA CCCGCAGGAATAATCCCTGAACGAATTCCTTTATTTGTATGATCGGTAGG GATTACTGCCTTACTTCTACTACTATCATTACCTGTGCTAGCAGGTGCTA TTACAATATTATTAACTGATCGAAATTTTAACACATCATTTTTTGATCCC TCAGGTGGAGGAGACCCTATTTTATACCAACATTTATTTTGATTTTTTGG TCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Pentatomidae was found to be postive for Wolbachia.
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