Sample information |
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| Picture |
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|---|---|
| Location | No map location data available. |
| Collection date | |
| Captive / Cultivated? | |
| Group | Georgia Southern University |
| Observations | |
| Putative identification | Arthropoda Insecta Coleoptera |
Methods |
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| Extraction kit | |
| DNA extraction location | savannah ga |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
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| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
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| Wolbachia presence | Unknown |
| Confidence level | Medium |
| Explanation of confidence level | Possible Wolbachia infection was shown in gel but PCR results came incocnlusive. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
AGCTGGTATAGTTGGAACTTCACTTAGACTTCTAATTCGAGCAGAATTAG GAAACCccttgtCTTTAATTGGTGATGATCAAATCTATAATGTAATTGTA AcatccCATGCTTTTATCATAATTTTTTTCATAGTAATACCAATTATAAT TGGTGGATTTGGAAATTGATTAGTACCTTTAATACTAGGAGCTCCTGATA TAGCATTTCCTCGAATAAACAATATGAGATTCTGGCTACTCCCCCCATCT CTTACTCTTCTTTTAATGAGAAGATTAGTAGAGAATGGAGCCGGAACTGG TTGAACAGTGTATCCCCCACTTTCAGCTAATATTGCCCATAGAGGAGCAT CAGTTGATTTAGCTATTTTTAGCCTTCATTTAGCTGGAATTAGGTCTATT TTAGGTGCAGTAAATTTTATTACTACAGTAATTAATATACGATCTACAGG AATAACATTTGATCGAATACCTTTATTTGTATGATCAGTAGTATTAACAG CATTACTCCTTTTACTTTCTTTGCCAGTTTTAGCTGGAGCTATTACAATA CTTCTAACAGATCGTAATATTAACACAACATTCTTTGACCCAGCAGGAGG TGGAGATCCTATTTTATATCAACACTTATTTTGATTTTTTGGTCA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid