ZET-HPR Diptera

November 11, 2025

Sample information
Picture
Photos by: Heer R.
Location
Collection date 08/26/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Location: lawn near the Science Center 
  • Habitat: Tall and dead grass lawn 
  • Method: Sweep Net
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Rear half
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

After examining the results from Functional Bioscience, no Wolbachia strain was detected.
Wolbachia 16S sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
TGATCTGGTATAGTAGGATCATCAATAAGATGAATTATTCGAATTGAACT AGGACAACCcgttTcagttATTGGAGATGATCAAATTTATAATGTTATTG TAACtgttCATGCCTTCATTATAATTTTTTTTATAGTAATACCTATTATA ATTGGAGGATTTGGTAATTGATTAGTACCTTTAATAATTGGAGCCCCTGA CATAGCATTTCCCCGAATAAATAATATAAGATTCTGATTATTACCCCCCT CTTTAATATTATTAATTATTAGAAGAACAGTGGAAATAGGGGTAGGCACT GGATGAACAGTTTACCCCCCATTATCAAGCAATTTATCACATAGAGGAGC CTCAGTTGATTTAGCAATCTTCTCCTTACATTTAGCAGGCATTTCATCAA TCTTAGGGGCAGTAAATTTTATTTCAACAATTATTAATATACGACCAAAG GGGATAATTTTAGAACGAACCCCATTATTCGTTTGATCTGTTGGAATTAC AGCCTTATTGCTACTATTATCTTTACCTGTATTAGCAGGGGCTATCACTA TATTATTAACTGATCGAAATTTTAATACTTCTTTCTTTGACCCTACTGGT GGTGGGGATCCTATTTTATATCAACACTTATTCTGATTTTTTGGTCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
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