DEM-LGN Blattodea

November 11, 2025

Sample information
Picture
Photos by: Lauren N
Location
Collection date 08/26/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Location: south of Science Center on Georgia Southern University Armstrong campus
  • Habitat: pine straw bedding at the base of a tree
  • Collection method: sweep net
Putative identification Arthropoda Insecta Blattodea Blaberidae

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

Visible bar aligned with Wolbachia 16S positive control, but it was very light.
Wolbachia 16S sequence
Arthropod COI sequence
TGGATAGTAGGGACATCTTTAAGAATATTAATCCGAGCAGAACTAAATCA ACCTgttTCATTAATTGGAGATGATCAAATCTATAATGTTATTGTAACGG CTCACGCTTTTGTTATAATTTTCTTTATAGTTATGCCAATTTTAATTGGA GGATTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGC ATTTCCTCGTATAAATAATATAAGTTTTTGACTTTTACCTCCATCTCTAT CTCTTCTATTAGCCAGTAGCCTTGTCGAAAGAGGAGCTGGGACTGGTTGA ACCGTATACCCTCCCTTAGCTAGAGGAATTGCTCATGCTGGAGCTTCAGT AGATTTAGCTATTTTCTCATTACATCTTGCAGGTGTATCATCAATTTTAG GTGCCGTAAATTTTATTTCAACAATTATTAACATGAAACCAATTAATATA AGCCCTGAACGAATTCCTTTATTTGTTTGGTCAGTAGGTATTACTGCATT ATTATTATTATTATCCTTACCAGTTCTTGCAGGTGCAATTACAATATTAT TAACAGACCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGGGGAGGT GATCCTATTTTATATCAACATTTATTTTGATTTTTTGGTC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Blaberidae was found to be postive for Wolbachia.
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