November 11, 2025

Sample information
Picture
Photos by: Abigail W.
Location No map location data available.
Collection date 08/26/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

 

Date Collected 8/26/25
Time Collected 1:35 PM
Location Of Collection  By Arts Drive by the mailroom
Habitat Garden, tall grasses, and weeds
Method of collection  Sweep Net
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

My confidence is high because of the uncultured bacterium that was prevalent in the DNA sequence.

 
Wolbachia 16S sequence
Arthropod COI sequence
AACCTCCCTTagaCTACTAATTcgagcagaACTcggaAatccccttttgg gtgATTGGAGAcgaCCAAATTTACAATGTTATTGttccttttcATGCTTT CATCATAATTTTTTTCATGGTAATACCAATTATAATTGGTGGATTCGGAA ACTGGCTTGTCCCTCTAATACTTGGGGCCCCTGACATagcTTTCCCCCGA ATAAATAACATAAGATTTTGGCTTCTTCCTCCTTCTTTGACTTTACTTTT AATGagaAGATTagtaGAAAGAGGAGCTGGAACTGGATGAACAGTATACC CCCCTTTATCTAGAAATATCGCTCACAGAGGGGCTTCTGTAGATCTAGCT ATTTTTAGTTTACACCTAGCTGGAATTTCATCTATTCTTGGAGCTGTAAA TTTCATTACTACAGTTATTAACATACGTTCTACAGGCATAACATTTGACC GAATACCATTATTTGTATGATCAGTAGCTTTAACaactCTTTTACTACTT CTGTCCCTGCCTGTCCTAGCTGGCGCAATCACGATACTTTTAACTGATCG AAATATTAATACCTCATTCTTTGACCCAGCGGGTGGTGGAGACCCAATTT TATACCAACACTTATTTTGATTTTTTGGTCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be postive for Wolbachia.
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