Sample information |
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| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 08/26/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Collection was August 26, 2025 and the insect was wild caught with the Ariel net weep method in a group of green prickly plants outside of the Learning Commons building. |
| Putative identification | Arthropoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
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| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | I was someone confident thinking wasps is a common insect to carry Wolbachia but at the same time I was not sure what to expect. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
ATTGGAACATCCTTAAGATTAATTATTCGATTAGAACTAGGAACTCCaat ttCAATAATCAATAATGATCAAATTTACAACTCAATCATTACAGCACATG CATTAATTATAATTTTTTTTATAGTTATACCATTTATAATTGGGGGGTTC GGAAATTGATTAATTCCATTAATATTAGGAGCCCCAGATATAGCTTTCCC ACGAATAAATAATATAAGATTCTGACTTCTACCACCATCTCTTATATTAT TATTAATAAGAAATATTAATGATATAGGAGTAGGAACAGGATGAACTTTA TATCCACCCCTATCATCAACTATAGGACACAATTCATCATCAGTAGATTT TGCTATTTTTTCACTCCACATTGCAGGAATTTCATCAATCATAGGAGCAA TTAATTTTATTGTAACCATCTTAAATATACATATTAAAACCCATTCATTA AATTTTTTACCTATATTTACATGATCAGTTTTAATCACAGCAATCTTATT ACTATTATCTTTACCAGTTCTAGCAGGAGCTATTACTATACTATTAACTG ACCGAAACTTAAATACATCATTTTTTGACCCTTTAGGAGGAGGAGACCCA ATTTTATTTCAACATTTATTTTGATTTTTTGGT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid